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glycine radical domain-containing pyruvate formate-lyase [Escherichia coli]
Protein Classification
glycyl radical protein ( domain architecture ID 11484545 )
glycyl radical protein similar to formate acetyltransferase, benzylsuccinate synthase, 4-hydroxyphenylacetate decarboxylase, and CutC choline trimethylamine-lyase
List of domain hits
Name
Accession
Description
Interval
E-value
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
1-765
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 1714.65
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE 80
Cdd:PRK09983 1 MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 81 MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID 160
Cdd:PRK09983 81 MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK 240
Cdd:PRK09983 161 YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 241 PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQ T SLTQGED A AFLKELLESLWVKCNDIVLLRSTSSARYFA 320
Cdd:PRK09983 241 PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQ A SLTQGED P AFLKELLESLWVKCNDIVLLRSTSSARYFA 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 321 GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA 400
Cdd:PRK09983 321 GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA 400
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 401 FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS 480
Cdd:PRK09983 401 FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS 480
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 481 NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANF 560
Cdd:PRK09983 481 NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANF 560
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 561 ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE 640
Cdd:PRK09983 561 ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE 640
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 641 QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD 720
Cdd:PRK09983 641 QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD 720
730 740 750 760
....*....|....*....|....*....|....*....|....*
gi 1617439169 721 TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL 765
Cdd:PRK09983 721 TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL 765
Name
Accession
Description
Interval
E-value
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
1-765
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 1714.65
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE 80
Cdd:PRK09983 1 MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 81 MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID 160
Cdd:PRK09983 81 MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK 240
Cdd:PRK09983 161 YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 241 PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQ T SLTQGED A AFLKELLESLWVKCNDIVLLRSTSSARYFA 320
Cdd:PRK09983 241 PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQ A SLTQGED P AFLKELLESLWVKCNDIVLLRSTSSARYFA 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 321 GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA 400
Cdd:PRK09983 321 GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA 400
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 401 FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS 480
Cdd:PRK09983 401 FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS 480
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 481 NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANF 560
Cdd:PRK09983 481 NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANF 560
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 561 ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE 640
Cdd:PRK09983 561 ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE 640
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 641 QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD 720
Cdd:PRK09983 641 QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD 720
730 740 750 760
....*....|....*....|....*....|....*....|....*
gi 1617439169 721 TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL 765
Cdd:PRK09983 721 TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL 765
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
1-765
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1352.65
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 MTN RI SR LK T AL FANTREI S L ERA L LYT A S HR QTEGEP V I L RRAKA T A YI LE HVE I S I R D E ELI A GN RTVK PRA G I MS PE 80
Cdd:TIGR01774 2 CMD RI EK LK K AL SKPVARL S V ERA R LYT E S MK QTEGEP M I I RRAKA L A HV LE NIP I Q I L D S ELI V GN MLPN PRA A I IF PE 81
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 81 MDPY W LL KELD QF P T RP QD RFA I SEEDKR IY REE LF PYW EKRSMK DF IN G Q MTDE V K AATS T Q IF SINQTDK G QG H IIID 160
Cdd:TIGR01774 82 GVGL W II KELD SL P N RP TN RFA V SEEDKR VL REE IA PYW QRKTIE DF AF G L MTDE M K ILYT T S IF VLTGEIA G IS H VAVN 161
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 YP R LL NH GL GE L V -------- A QMQQHCQQQPENH FYQAA LLL LEA SQK HILRYA E LAE T MAA NC T DAQ RREELL T IAEI 232
Cdd:TIGR01774 162 YP Y LL RR GL RW L L eeserrir A LEESGVYEGEKYS FYQAA KIV LEA VIN HILRYA K LAE E MAA SE T GES RREELL K IAEI 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 233 S R HN A QH KPQTFWQA C QL F W YMNI ILQ Y ESN AS S L S L GR F DQY ML PFY QTSLTQ G E - D AAFLK E L L E SLW V K C N D IV LL R 311
Cdd:TIGR01774 242 C R KV A AE KPQTFWQA V QL V W LVQS ILQ Q ESN EQ S I S M GR I DQY LY PFY KKDIGE G R i D RELAF E I L A SLW I K T N E IV PA R 321
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 312 S T S SAR YFAG F PT GYTALL GG LTEN G RS AVN V LS F L C L DAYQSVQ L P QPNL G VR T NA LIDTP FL MKT AE T IR L G T G I P QI 391
Cdd:TIGR01774 322 S S S LEQ YFAG Q PT NQAVTI GG CDIY G ND AVN E LS Y L M L EVTDRLR L R QPNL H VR I NA GSPES FL KRL AE A IR S G C G N P AL 401
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 392 FNDE V VVPAF L N R GV SLE DA R DY SVV GCVE LSIP G RT Y GLH D I A MF N LL KVME IC L H E NE ---- G N ------- AALTY E G 460
Cdd:TIGR01774 402 FNDE A VVPAF K N A GV DDR DA L DY TTD GCVE IAPF G NS Y TSS D A A LI N VA KVME YA L N E GV dlqf G Y efgakte KPKFL E D 481
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 461 LLE QI R AK ISHY IT L M VEGSN IC D IGHRDWA P V PLLS SFIS DC L EKG R DI TD GGARYNF S G V Q GI GIANL S DSL H A L KG M 540
Cdd:TIGR01774 482 LLE KL R EQ ISHY VK L V VEGSN VL D YANAEVK P T PLLS LCVE DC F EKG V DI SR GGARYNF T G I Q AV GIANL G DSL V A I KG A 561
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 541 VF D Q QRL S F D E L LSV L KA NF ATPEG E KV R A RLIN RFE KYGND I D E VD NIS A EL L RH YC K EV EK Y Q NPRGG Y F TP G S Y TV S 620
Cdd:TIGR01774 562 VF E Q GAY S M D D L VEA L RK NF VGLTH E EK R H RLIN QSP KYGND D D A VD KYT A MV L EW YC E EV NR Y R NPRGG K F AA G C Y PM S 641
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 621 A H VP L G SVVG ATPDGR F AGE Q LA D g G L SP ML G Q D AQ GPTAV LK SVSKL DNTL L S NG T LLN V K FT PATLE G E AGLR KL ADF 700
Cdd:TIGR01774 642 A N VP F G FFTS ATPDGR K AGE P LA P - G V SP ST G M D RE GPTAV IN SVSKL SYEN L P NG A LLN I K LS PATLE E E GDKQ KL IEA 720
730 740 750 760 770 780
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1617439169 701 LR - A F TQ LK LQ HIQFN V V NA DTLR E AQQ R P QD Y AG LVVRVAGYSAFFVELS KEI QDDIIRRT A H Q L 765
Cdd:TIGR01774 721 LR k S F ME LK VM HIQFN I V KE DTLR K AQQ E P EK Y RW LVVRVAGYSAFFVELS RPV QDDIIRRT S H R L 786
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
2-762
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1141.24
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 2 T N RI S RLK TALFANTRE IS L ERA L LYT A S HRQ TEG E PVI L RRAKA TAY ILE HVE I S I R D E ELI A GNR TV KPRA GIMS PE M 81
Cdd:cd01677 1 T E RI K RLK AKILTAKPS IS I ERA R LYT E S YKE TEG K PVI I RRAKA LKH ILE NAT I Y I Q D D ELI V GNR GG KPRA VPVF PE L 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 82 DPY W LLK ELD QF P T RP Q D R F A ISE e DK RI Y R EE L FPYW EKRSMK D FINGQMTD E VKA A TSTQI F SINQT - DK G Q GH III D 160
Cdd:cd01677 81 SVH W VED ELD DL P K RP G D P F V ISE - DK KE Y L EE I FPYW KGKTLR D RCFKYFPE E TLI A MAAGV F TEFMY f FS G P GH VAV D 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 YP RL L NH GL GE L V -------- A QMQQHCQQQPENH FYQA ALLLL EA SQKHIL RYAELA ETM AA NC TD AQ R RE ELL T IAEI 232
Cdd:cd01677 160 YP KV L EK GL DG L I eeakeaie A LDLTGPEDIDKIY FYQA MIIVC EA VITYAK RYAELA KEL AA KE TD PK R KA ELL E IAEI 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 233 S R HNAQ H K P Q TFW Q A C Q L FW YMNI ILQ Y ESN AS S L S L GRFDQY ML PFY QTSLTQ G E - DAAFLK ELLE S LW V K C N D I VLL R 311
Cdd:cd01677 240 C R RVPA H P P R TFW E A L Q S FW FIHL ILQ I ESN GH S I S P GRFDQY LY PFY KQDIEE G R l TREGAI ELLE C LW I K I N E I NKV R 319
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 312 S TS SA R YFAG FP T GYTALL GG L TE N G RS A V N V LS F L C L D A YQS V Q LPQP N L G VR TN A LIDTP FL M K T AE T IRLG T G I P QI 391
Cdd:cd01677 320 S GA SA K YFAG YN T FQNLTI GG Q TE D G SD A T N E LS Y L I L E A TRR V R LPQP S L T VR YH A KSPDK FL K K A AE V IRLG L G Y P AF 399
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 392 FNDEVV V PA F L NR GVSLEDARDY SVV GCVE LSI PGR T Y GLHDIAMF NL L KV M EI C L HENEGNAA -------------- L T 457
Cdd:cd01677 400 FNDEVV I PA L L RK GVSLEDARDY GLI GCVE TGA PGR K Y RWTGTGYI NL A KV L EI T L NNGKDPRS gkqvgpetgdatdf K T 479
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 458 Y E G L L E QIRAKIS H Y I T L M V EGS NI C DI G H RDW AP V P L LS SFIS DC L EKG R DI TD GGARYNF S G V QG I GIA N L S DSL H A L 537
Cdd:cd01677 480 F E E L W E AFKKQLR H F I K L S V RAN NI S DI A H AEV AP A P F LS ALVD DC I EKG K DI NA GGARYNF G G I QG V GIA T L G DSL A A I 559
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 538 K GM VF DQQR L SFD ELL SV LKANFA tp EG EKV R A RL I N RFE KYGND I D EV DNI SAELLRH YCKEVEKYQNPRGG Y F T PG S Y 617
Cdd:cd01677 560 K KL VF EEKK L TME ELL EA LKANFA -- EG YEE R R RL L N NAP KYGND D D YA DNI ARRVYEW YCKEVEKYQNPRGG K F Y PG T Y 637
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 618 T VSA H VP L GSV V GATPDGR F AG EQ L A DG GLSPM l G Q D AQ GPTAV L KSVSKLD NTLL S N GTLLN V KF T P A TLEGE A GL R KL 697
Cdd:cd01677 638 S VSA N VP F GSV T GATPDGR L AG TP L S DG VSPSQ - G T D KK GPTAV I KSVSKLD HFNI S G GTLLN Q KF S P S TLEGE E GL K KL 716
730 740 750 760 770 780
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1617439169 698 A DFL R AFTQ L KLQ HIQFNVV N A D TLR E AQ QR P QD Y AG L V VRVAGYSA F FVELSKE I QD D II R RT A 762
Cdd:cd01677 717 A ALI R TYFD L GGH HIQFNVV S A E TLR D AQ KH P EK Y RD L I VRVAGYSA Y FVELSKE V QD E II A RT E 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
1-765
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1014.31
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 M T N R IS RL KTA L FANTRE I SL ERA L L Y T A S HRQ TEG E PVI L RRAKA TAY ILEH VE I S I R D E ELI A G NR T V KPR AGIMS PE 80
Cdd:COG1882 7 P T E R TK RL REK L LEAKPL I DI ERA R L F T E S YKE TEG L PVI I RRAKA FSH ILEH KP I Y I K D D ELI V G LQ T D KPR KRPIF PE 86
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 81 MDPY W LLK ELD QF PTRPQD R F A IS E EDK R I Y RE e LF PYW EKRSMK D FINGQMTD E VKA A TSTQ I FSINQTDK G Q GHII I D 160
Cdd:COG1882 87 GGIR W VED ELD AL PTRPQD G F E IS P EDK E I F RE - IA PYW KGKTHN D GVFDAYPE E IRK A RKAG I ITGLPDAY G R GHII G D 165
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 Y P R L L NH GL GE L VAQ -------- MQQHCQQQPENH FY Q A ALLLL EA SQKHIL RYAELA ETM A ANC TD AQ R RE ELL T IAEI 232
Cdd:COG1882 166 Y R R V L LY GL DG L IEE akeklael DLTDPEDIEKID FY K A MIIVC EA VIRLAE RYAELA REL A EKE TD PK R KA ELL E IAEI 245
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 233 SRHNAQHKPQ TFW Q A C Q LF W YMNIILQY E S N ASSL SLGRFDQY ML P F Y QTS L TQ G --- E DA A fl K ELL ESL W V K CNDIVL 309
Cdd:COG1882 246 CGFVPANPAR TFW E A V Q WV W FVYLAAIK E Q N GAAM SLGRFDQY LY P Y Y ERD L EE G rlt E EE A -- Q ELL DCF W I K LREVRF 323
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 310 LR STSS A RY FAG F PT GY T ALL GG L T EN GR S AVN V LS F L C L DAYQSVQ LP Q PNL G VR TNALIDTP FL M K T AE T I RL GTG I P 389
Cdd:COG1882 324 LR TPEY A EL FAG Y PT WV T LTI GG M T PD GR D AVN E LS Y L I L ETLRNLP LP E PNL T VR WSEKLPEG FL K K A AE V I SI GTG S P 403
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 390 Q IF ND EVVV P AF LN R GV S LEDARDY SVV GCVE LSI PG RTYGLHDIAMF NL L K VM E IC L HENE ------------ G NAA -- 455
Cdd:COG1882 404 Q YE ND DLMI P ML LN K GV T LEDARDY GIA GCVE PMV PG KQMQFFGAGRI NL A K AL E YA L NNGV dektgkqvgpet G DPT df 483
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 456 LTY EGLL E QIRAKISHYIT L M V EGS NI CDIG H RDW AP V P L LS SFIS DC L E K G R D ITD GGARYNF SGVQGI G IANLS DSL H 535
Cdd:COG1882 484 LTY DEVM E AFKKQLDYLAD L Y V NAL NI IHYM H DKY AP E P F LS ALHD DC I E R G K D LNE GGARYNF GAIGIA G LSVVA DSL S 563
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 536 A L K GM VFD QQRLSF DELL SV L K ANF atp EG - E KV R AR L I N r FE KYGND I D E VD N I SA EL LRHYCK E VE KY QNP RGG YF T P 614
Cdd:COG1882 564 A I K KL VFD KKKVTM DELL EA L A ANF --- EG y E EL R QL L L N - AP KYGND D D Y VD E I AV EL VETFMD E IR KY KTY RGG TY T L 639
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 615 GSY T VSAH VP L G SVV GATPDGR F AGE Q LAD g G L SPM L G Q D AQ GPTAVLKSV S KL DNTLLSN G T LLN V KF T P AT L E GE A G L 694
Cdd:COG1882 640 SIL T ITSN VP Y G KKT GATPDGR K AGE P LAD - G A SPM H G R D KN GPTAVLKSV A KL PYEKATD G I LLN Q KF S P SA L G GE E G I 718
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1617439169 695 RK L ADF LR AFTQ L KLQ HIQFNVV NAD TL RE AQ QR P QD Y AG L V VRVAGYSA F FVELSKE I QDDII R RT A H QL 765
Cdd:COG1882 719 EN L VSL LR TYFD L GGH HIQFNVV DRE TL LD AQ KH P EK Y PD L T VRVAGYSA Y FVELSKE Q QDDII A RT E H EF 789
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
1-626
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 747.53
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 M TN RI SR LK -- TA L FANTREI S L ERA L L Y T A S HRQ TEG E - PV IL RRAKA TAY IL E H VEIS IRD E ELI A G NR T V KPR AGIM 77
Cdd:pfam02901 1 M WE RI DV LK en YT L YTGDPSL S W ERA R L L T E S YKE TEG V l PV DI RRAKA LKK IL S H LPGY IRD D ELI V G LQ T D KPR KRAI 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 78 S PE MDPY W LLK ELD QFP TRPQD R F A ISEEDK R I Y RE e L FPYW EKRSMKDFINGQM T D E V KAA TSTQ IF SINQTDK G Q GHI 157
Cdd:pfam02901 81 Y PE GGIR W VED ELD YLN TRPQD G F E ISEEDK K I F RE - I FPYW KGKTHNEGVFDAY T P E M KAA RESG IF TGLPDAY G R GHI 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 158 I I DY P R L L NH GL GE L VAQMQQHCQQQPEN ------- H FY Q A ALLLLE A SQKHIL RYA E LAE TM A ANC TD AQ R RE ELL T IA 230
Cdd:pfam02901 160 I G DY R R V L LY GL DG L IEEKEEKLAKLDTD pedieki E FY K A MIISCD A VIEYAE RYA R LAE EL A EQE TD PK R KA ELL E IA 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 231 EI SRHNAQHKPQ TF WQ A C Q L FW YMNIILQY E S N ASSL SLGR F DQY ML P F Y QTS L TQ G --- E DA A F lk EL LESL W V K CNDI 307
Cdd:pfam02901 240 EI CGRVPARPAE TF QE A I Q W FW FVYLAAVK E Q N GAAM SLGR L DQY LY P Y Y ERD L EE G rlt E EE A Q -- EL IDCF W I K LREV 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 308 VL LR STSSARY FAG FPTGYTALL GG LTEN GR S AVN V LS F L C L D A YQSVQ LP Q PNL G VR TNALIDTP FL M K T AE TI R L GTG 387
Cdd:pfam02901 318 RF LR TPEYNKL FAG YDPFQNLTI GG QGRD GR D AVN K LS Y L I L E A LDNLP LP E PNL T VR WSKKLPEE FL K K A AE VS R K GTG 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 388 I PQ IF ND E V VV PA F LNRGVSLEDARDY SVV GCVE LSI PG RTYGLH d I A MF NL L K VM E IC L HE -------------- NEGN 453
Cdd:pfam02901 398 S PQ YE ND D V MI PA L LNRGVSLEDARDY GIA GCVE PMK PG KEMQFF - G A RI NL A K AL E YA L NG grdeltgkqvgpkt GPVT 476
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 454 AA L TY E GLL E QIRAKISHYIT L M V EGS NI CDIG H RDW AP V P L LS SFIS DC L E K G R D ITD GGARYNFSG V QG I G I AN LS DS 533
Cdd:pfam02901 477 EF L SF E EVM E AFKKQLDYLAD L Y V NAL NI IHYM H DKY AP E P F LS ALHD DC I E R G K D VGI GGARYNFSG P QG A G L AN VA DS 556
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 534 L H A L K GM VFD QQRLSFD EL LSV L K A N F ATP eg E KV R AR L I N RFE KYGND I D E VD N I SA E LLRHYCK EV E KY Q N P RGG Y FT 613
Cdd:pfam02901 557 L S A I K KL VFD DKVYTLR EL EDA L A A D F EGE -- E EL R QD L L N DAP KYGND D D R VD D I AV E VVETFMD EV R KY K N Y RGG K FT 634
650
....*....|...
gi 1617439169 614 P GSY T VSAH VP L G 626
Cdd:pfam02901 635 P SLL T ITSN VP Y G 647
Name
Accession
Description
Interval
E-value
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
1-765
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 1714.65
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE 80
Cdd:PRK09983 1 MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 81 MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID 160
Cdd:PRK09983 81 MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK 240
Cdd:PRK09983 161 YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 241 PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQ T SLTQGED A AFLKELLESLWVKCNDIVLLRSTSSARYFA 320
Cdd:PRK09983 241 PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQ A SLTQGED P AFLKELLESLWVKCNDIVLLRSTSSARYFA 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 321 GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA 400
Cdd:PRK09983 321 GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA 400
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 401 FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS 480
Cdd:PRK09983 401 FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS 480
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 481 NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANF 560
Cdd:PRK09983 481 NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANF 560
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 561 ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE 640
Cdd:PRK09983 561 ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE 640
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 641 QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD 720
Cdd:PRK09983 641 QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD 720
730 740 750 760
....*....|....*....|....*....|....*....|....*
gi 1617439169 721 TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL 765
Cdd:PRK09983 721 TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL 765
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
1-765
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1352.65
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 MTN RI SR LK T AL FANTREI S L ERA L LYT A S HR QTEGEP V I L RRAKA T A YI LE HVE I S I R D E ELI A GN RTVK PRA G I MS PE 80
Cdd:TIGR01774 2 CMD RI EK LK K AL SKPVARL S V ERA R LYT E S MK QTEGEP M I I RRAKA L A HV LE NIP I Q I L D S ELI V GN MLPN PRA A I IF PE 81
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 81 MDPY W LL KELD QF P T RP QD RFA I SEEDKR IY REE LF PYW EKRSMK DF IN G Q MTDE V K AATS T Q IF SINQTDK G QG H IIID 160
Cdd:TIGR01774 82 GVGL W II KELD SL P N RP TN RFA V SEEDKR VL REE IA PYW QRKTIE DF AF G L MTDE M K ILYT T S IF VLTGEIA G IS H VAVN 161
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 YP R LL NH GL GE L V -------- A QMQQHCQQQPENH FYQAA LLL LEA SQK HILRYA E LAE T MAA NC T DAQ RREELL T IAEI 232
Cdd:TIGR01774 162 YP Y LL RR GL RW L L eeserrir A LEESGVYEGEKYS FYQAA KIV LEA VIN HILRYA K LAE E MAA SE T GES RREELL K IAEI 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 233 S R HN A QH KPQTFWQA C QL F W YMNI ILQ Y ESN AS S L S L GR F DQY ML PFY QTSLTQ G E - D AAFLK E L L E SLW V K C N D IV LL R 311
Cdd:TIGR01774 242 C R KV A AE KPQTFWQA V QL V W LVQS ILQ Q ESN EQ S I S M GR I DQY LY PFY KKDIGE G R i D RELAF E I L A SLW I K T N E IV PA R 321
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 312 S T S SAR YFAG F PT GYTALL GG LTEN G RS AVN V LS F L C L DAYQSVQ L P QPNL G VR T NA LIDTP FL MKT AE T IR L G T G I P QI 391
Cdd:TIGR01774 322 S S S LEQ YFAG Q PT NQAVTI GG CDIY G ND AVN E LS Y L M L EVTDRLR L R QPNL H VR I NA GSPES FL KRL AE A IR S G C G N P AL 401
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 392 FNDE V VVPAF L N R GV SLE DA R DY SVV GCVE LSIP G RT Y GLH D I A MF N LL KVME IC L H E NE ---- G N ------- AALTY E G 460
Cdd:TIGR01774 402 FNDE A VVPAF K N A GV DDR DA L DY TTD GCVE IAPF G NS Y TSS D A A LI N VA KVME YA L N E GV dlqf G Y efgakte KPKFL E D 481
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 461 LLE QI R AK ISHY IT L M VEGSN IC D IGHRDWA P V PLLS SFIS DC L EKG R DI TD GGARYNF S G V Q GI GIANL S DSL H A L KG M 540
Cdd:TIGR01774 482 LLE KL R EQ ISHY VK L V VEGSN VL D YANAEVK P T PLLS LCVE DC F EKG V DI SR GGARYNF T G I Q AV GIANL G DSL V A I KG A 561
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 541 VF D Q QRL S F D E L LSV L KA NF ATPEG E KV R A RLIN RFE KYGND I D E VD NIS A EL L RH YC K EV EK Y Q NPRGG Y F TP G S Y TV S 620
Cdd:TIGR01774 562 VF E Q GAY S M D D L VEA L RK NF VGLTH E EK R H RLIN QSP KYGND D D A VD KYT A MV L EW YC E EV NR Y R NPRGG K F AA G C Y PM S 641
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 621 A H VP L G SVVG ATPDGR F AGE Q LA D g G L SP ML G Q D AQ GPTAV LK SVSKL DNTL L S NG T LLN V K FT PATLE G E AGLR KL ADF 700
Cdd:TIGR01774 642 A N VP F G FFTS ATPDGR K AGE P LA P - G V SP ST G M D RE GPTAV IN SVSKL SYEN L P NG A LLN I K LS PATLE E E GDKQ KL IEA 720
730 740 750 760 770 780
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1617439169 701 LR - A F TQ LK LQ HIQFN V V NA DTLR E AQQ R P QD Y AG LVVRVAGYSAFFVELS KEI QDDIIRRT A H Q L 765
Cdd:TIGR01774 721 LR k S F ME LK VM HIQFN I V KE DTLR K AQQ E P EK Y RW LVVRVAGYSAFFVELS RPV QDDIIRRT S H R L 786
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
2-762
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1141.24
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 2 T N RI S RLK TALFANTRE IS L ERA L LYT A S HRQ TEG E PVI L RRAKA TAY ILE HVE I S I R D E ELI A GNR TV KPRA GIMS PE M 81
Cdd:cd01677 1 T E RI K RLK AKILTAKPS IS I ERA R LYT E S YKE TEG K PVI I RRAKA LKH ILE NAT I Y I Q D D ELI V GNR GG KPRA VPVF PE L 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 82 DPY W LLK ELD QF P T RP Q D R F A ISE e DK RI Y R EE L FPYW EKRSMK D FINGQMTD E VKA A TSTQI F SINQT - DK G Q GH III D 160
Cdd:cd01677 81 SVH W VED ELD DL P K RP G D P F V ISE - DK KE Y L EE I FPYW KGKTLR D RCFKYFPE E TLI A MAAGV F TEFMY f FS G P GH VAV D 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 YP RL L NH GL GE L V -------- A QMQQHCQQQPENH FYQA ALLLL EA SQKHIL RYAELA ETM AA NC TD AQ R RE ELL T IAEI 232
Cdd:cd01677 160 YP KV L EK GL DG L I eeakeaie A LDLTGPEDIDKIY FYQA MIIVC EA VITYAK RYAELA KEL AA KE TD PK R KA ELL E IAEI 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 233 S R HNAQ H K P Q TFW Q A C Q L FW YMNI ILQ Y ESN AS S L S L GRFDQY ML PFY QTSLTQ G E - DAAFLK ELLE S LW V K C N D I VLL R 311
Cdd:cd01677 240 C R RVPA H P P R TFW E A L Q S FW FIHL ILQ I ESN GH S I S P GRFDQY LY PFY KQDIEE G R l TREGAI ELLE C LW I K I N E I NKV R 319
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 312 S TS SA R YFAG FP T GYTALL GG L TE N G RS A V N V LS F L C L D A YQS V Q LPQP N L G VR TN A LIDTP FL M K T AE T IRLG T G I P QI 391
Cdd:cd01677 320 S GA SA K YFAG YN T FQNLTI GG Q TE D G SD A T N E LS Y L I L E A TRR V R LPQP S L T VR YH A KSPDK FL K K A AE V IRLG L G Y P AF 399
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 392 FNDEVV V PA F L NR GVSLEDARDY SVV GCVE LSI PGR T Y GLHDIAMF NL L KV M EI C L HENEGNAA -------------- L T 457
Cdd:cd01677 400 FNDEVV I PA L L RK GVSLEDARDY GLI GCVE TGA PGR K Y RWTGTGYI NL A KV L EI T L NNGKDPRS gkqvgpetgdatdf K T 479
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 458 Y E G L L E QIRAKIS H Y I T L M V EGS NI C DI G H RDW AP V P L LS SFIS DC L EKG R DI TD GGARYNF S G V QG I GIA N L S DSL H A L 537
Cdd:cd01677 480 F E E L W E AFKKQLR H F I K L S V RAN NI S DI A H AEV AP A P F LS ALVD DC I EKG K DI NA GGARYNF G G I QG V GIA T L G DSL A A I 559
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 538 K GM VF DQQR L SFD ELL SV LKANFA tp EG EKV R A RL I N RFE KYGND I D EV DNI SAELLRH YCKEVEKYQNPRGG Y F T PG S Y 617
Cdd:cd01677 560 K KL VF EEKK L TME ELL EA LKANFA -- EG YEE R R RL L N NAP KYGND D D YA DNI ARRVYEW YCKEVEKYQNPRGG K F Y PG T Y 637
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 618 T VSA H VP L GSV V GATPDGR F AG EQ L A DG GLSPM l G Q D AQ GPTAV L KSVSKLD NTLL S N GTLLN V KF T P A TLEGE A GL R KL 697
Cdd:cd01677 638 S VSA N VP F GSV T GATPDGR L AG TP L S DG VSPSQ - G T D KK GPTAV I KSVSKLD HFNI S G GTLLN Q KF S P S TLEGE E GL K KL 716
730 740 750 760 770 780
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1617439169 698 A DFL R AFTQ L KLQ HIQFNVV N A D TLR E AQ QR P QD Y AG L V VRVAGYSA F FVELSKE I QD D II R RT A 762
Cdd:cd01677 717 A ALI R TYFD L GGH HIQFNVV S A E TLR D AQ KH P EK Y RD L I VRVAGYSA Y FVELSKE V QD E II A RT E 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
1-765
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1014.31
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 M T N R IS RL KTA L FANTRE I SL ERA L L Y T A S HRQ TEG E PVI L RRAKA TAY ILEH VE I S I R D E ELI A G NR T V KPR AGIMS PE 80
Cdd:COG1882 7 P T E R TK RL REK L LEAKPL I DI ERA R L F T E S YKE TEG L PVI I RRAKA FSH ILEH KP I Y I K D D ELI V G LQ T D KPR KRPIF PE 86
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 81 MDPY W LLK ELD QF PTRPQD R F A IS E EDK R I Y RE e LF PYW EKRSMK D FINGQMTD E VKA A TSTQ I FSINQTDK G Q GHII I D 160
Cdd:COG1882 87 GGIR W VED ELD AL PTRPQD G F E IS P EDK E I F RE - IA PYW KGKTHN D GVFDAYPE E IRK A RKAG I ITGLPDAY G R GHII G D 165
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 161 Y P R L L NH GL GE L VAQ -------- MQQHCQQQPENH FY Q A ALLLL EA SQKHIL RYAELA ETM A ANC TD AQ R RE ELL T IAEI 232
Cdd:COG1882 166 Y R R V L LY GL DG L IEE akeklael DLTDPEDIEKID FY K A MIIVC EA VIRLAE RYAELA REL A EKE TD PK R KA ELL E IAEI 245
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 233 SRHNAQHKPQ TFW Q A C Q LF W YMNIILQY E S N ASSL SLGRFDQY ML P F Y QTS L TQ G --- E DA A fl K ELL ESL W V K CNDIVL 309
Cdd:COG1882 246 CGFVPANPAR TFW E A V Q WV W FVYLAAIK E Q N GAAM SLGRFDQY LY P Y Y ERD L EE G rlt E EE A -- Q ELL DCF W I K LREVRF 323
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 310 LR STSS A RY FAG F PT GY T ALL GG L T EN GR S AVN V LS F L C L DAYQSVQ LP Q PNL G VR TNALIDTP FL M K T AE T I RL GTG I P 389
Cdd:COG1882 324 LR TPEY A EL FAG Y PT WV T LTI GG M T PD GR D AVN E LS Y L I L ETLRNLP LP E PNL T VR WSEKLPEG FL K K A AE V I SI GTG S P 403
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 390 Q IF ND EVVV P AF LN R GV S LEDARDY SVV GCVE LSI PG RTYGLHDIAMF NL L K VM E IC L HENE ------------ G NAA -- 455
Cdd:COG1882 404 Q YE ND DLMI P ML LN K GV T LEDARDY GIA GCVE PMV PG KQMQFFGAGRI NL A K AL E YA L NNGV dektgkqvgpet G DPT df 483
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 456 LTY EGLL E QIRAKISHYIT L M V EGS NI CDIG H RDW AP V P L LS SFIS DC L E K G R D ITD GGARYNF SGVQGI G IANLS DSL H 535
Cdd:COG1882 484 LTY DEVM E AFKKQLDYLAD L Y V NAL NI IHYM H DKY AP E P F LS ALHD DC I E R G K D LNE GGARYNF GAIGIA G LSVVA DSL S 563
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 536 A L K GM VFD QQRLSF DELL SV L K ANF atp EG - E KV R AR L I N r FE KYGND I D E VD N I SA EL LRHYCK E VE KY QNP RGG YF T P 614
Cdd:COG1882 564 A I K KL VFD KKKVTM DELL EA L A ANF --- EG y E EL R QL L L N - AP KYGND D D Y VD E I AV EL VETFMD E IR KY KTY RGG TY T L 639
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 615 GSY T VSAH VP L G SVV GATPDGR F AGE Q LAD g G L SPM L G Q D AQ GPTAVLKSV S KL DNTLLSN G T LLN V KF T P AT L E GE A G L 694
Cdd:COG1882 640 SIL T ITSN VP Y G KKT GATPDGR K AGE P LAD - G A SPM H G R D KN GPTAVLKSV A KL PYEKATD G I LLN Q KF S P SA L G GE E G I 718
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1617439169 695 RK L ADF LR AFTQ L KLQ HIQFNVV NAD TL RE AQ QR P QD Y AG L V VRVAGYSA F FVELSKE I QDDII R RT A H QL 765
Cdd:COG1882 719 EN L VSL LR TYFD L GGH HIQFNVV DRE TL LD AQ KH P EK Y PD L T VRVAGYSA Y FVELSKE Q QDDII A RT E H EF 789
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
1-626
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 747.53
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 1 M TN RI SR LK -- TA L FANTREI S L ERA L L Y T A S HRQ TEG E - PV IL RRAKA TAY IL E H VEIS IRD E ELI A G NR T V KPR AGIM 77
Cdd:pfam02901 1 M WE RI DV LK en YT L YTGDPSL S W ERA R L L T E S YKE TEG V l PV DI RRAKA LKK IL S H LPGY IRD D ELI V G LQ T D KPR KRAI 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 78 S PE MDPY W LLK ELD QFP TRPQD R F A ISEEDK R I Y RE e L FPYW EKRSMKDFINGQM T D E V KAA TSTQ IF SINQTDK G Q GHI 157
Cdd:pfam02901 81 Y PE GGIR W VED ELD YLN TRPQD G F E ISEEDK K I F RE - I FPYW KGKTHNEGVFDAY T P E M KAA RESG IF TGLPDAY G R GHI 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 158 I I DY P R L L NH GL GE L VAQMQQHCQQQPEN ------- H FY Q A ALLLLE A SQKHIL RYA E LAE TM A ANC TD AQ R RE ELL T IA 230
Cdd:pfam02901 160 I G DY R R V L LY GL DG L IEEKEEKLAKLDTD pedieki E FY K A MIISCD A VIEYAE RYA R LAE EL A EQE TD PK R KA ELL E IA 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 231 EI SRHNAQHKPQ TF WQ A C Q L FW YMNIILQY E S N ASSL SLGR F DQY ML P F Y QTS L TQ G --- E DA A F lk EL LESL W V K CNDI 307
Cdd:pfam02901 240 EI CGRVPARPAE TF QE A I Q W FW FVYLAAVK E Q N GAAM SLGR L DQY LY P Y Y ERD L EE G rlt E EE A Q -- EL IDCF W I K LREV 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 308 VL LR STSSARY FAG FPTGYTALL GG LTEN GR S AVN V LS F L C L D A YQSVQ LP Q PNL G VR TNALIDTP FL M K T AE TI R L GTG 387
Cdd:pfam02901 318 RF LR TPEYNKL FAG YDPFQNLTI GG QGRD GR D AVN K LS Y L I L E A LDNLP LP E PNL T VR WSKKLPEE FL K K A AE VS R K GTG 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 388 I PQ IF ND E V VV PA F LNRGVSLEDARDY SVV GCVE LSI PG RTYGLH d I A MF NL L K VM E IC L HE -------------- NEGN 453
Cdd:pfam02901 398 S PQ YE ND D V MI PA L LNRGVSLEDARDY GIA GCVE PMK PG KEMQFF - G A RI NL A K AL E YA L NG grdeltgkqvgpkt GPVT 476
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 454 AA L TY E GLL E QIRAKISHYIT L M V EGS NI CDIG H RDW AP V P L LS SFIS DC L E K G R D ITD GGARYNFSG V QG I G I AN LS DS 533
Cdd:pfam02901 477 EF L SF E EVM E AFKKQLDYLAD L Y V NAL NI IHYM H DKY AP E P F LS ALHD DC I E R G K D VGI GGARYNFSG P QG A G L AN VA DS 556
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 534 L H A L K GM VFD QQRLSFD EL LSV L K A N F ATP eg E KV R AR L I N RFE KYGND I D E VD N I SA E LLRHYCK EV E KY Q N P RGG Y FT 613
Cdd:pfam02901 557 L S A I K KL VFD DKVYTLR EL EDA L A A D F EGE -- E EL R QD L L N DAP KYGND D D R VD D I AV E VVETFMD EV R KY K N Y RGG K FT 634
650
....*....|...
gi 1617439169 614 P GSY T VSAH VP L G 626
Cdd:pfam02901 635 P SLL T ITSN VP Y G 647
choline_CutC
TIGR04394 choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and ...
2-761
4.62e-174
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and benzylsuccinate synthases, are glycine radical enzymes that appear to act as choline TMA-lyase, that is, to perform a C-N bond cleavage turning choline into trimethylamine (TMA) plus acetaldehyde. The gene symbol is cutC, for choline utilization. The activase, CutD, is a radical SAM enzyme. [Energy metabolism, Amino acids and amines]
Pssm-ID: 275187 [Multi-domain]
Cd Length: 789
Bit Score: 519.36
E-value: 4.62e-174
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 2 T N R IS RLK TALFANTRE I SLE RA LLY T ASHRQTE G E P V IL R RAKA TAYIL E HVEIS I R D E ELI A G NRTVK PRAG IM SP EM 81
Cdd:TIGR04394 2 T E R LV RLK ENYLKQVPS I TIY RA RAI T EIAKENP G M P K IL L RAKA FRKCC E TAPLV I Q D D ELI V G APCGA PRAG AF SP DI 81
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 82 DPY WL LK ELD QFP TRPQD R F A ISEEDK R I Y REE L FP Y WE KR S MKDFING Q MTD ---- E VKAATSTQIF S INQTDK G ---- 153
Cdd:TIGR04394 82 AWR WL RD ELD TIG TRPQD P F Y ISEEDK K I M REE I FP F WE GK S LDEYCED Q YRE agvw E LSGESFVSDC S YHAVNG G gdsn 161
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 154 Q G HII I DYPRLLNHGLG E LVAQMQQHCQQQ PE N ---- H FY QAALLLL E ASQKHIL R YA E L A ETM AA NCT D AQ R RE EL LT I 229
Cdd:TIGR04394 162 P G YDV I LMKKGMLDIQA E AKEHLAELSYEN PE D idki Y FY KSVIDTT E GVMIYAK R LS E Y A AEL AA KEQ D PK R KA EL EK I 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 230 AE ISRHNAQ HKP Q TFW Q A C Q LF W YMNII L QY E S N ASSL S L GR F DQYM L PFY QTSLTQ G --- E DA AF lk EL LESLWV K CND 306
Cdd:TIGR04394 242 AE VNARVPA HKP R TFW E A I Q SV W TVESL L VV E E N QTGM S I GR V DQYM Y PFY KADIEA G rmt E YE AF -- EL AGCMLI K MSE 319
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 307 IVL L R S TSSARY FAG FPTGYTALL GG L T EN G RS A V N V L SF L CL DA YQS V QLP QP N L GV R TNALIDTPF L M K TAETI R L G T 386
Cdd:TIGR04394 320 MMW L T S EGGSKF FAG YQPFVNMCV GG V T RE G GD A T N D L TY L LM DA VRH V KVY QP S L AC R IHNKSPQKY L K K IVDVV R A G M 399
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 387 G I P QIFN D EVVVPAF L NR GVS L EDARDY SVV GCVE LSIP GR T Y GLHDI A MFNLLKVM E IC L he N E G NAAL ---------- 456
Cdd:TIGR04394 400 G F P ACHF D DAHIKMM L AK GVS I EDARDY CLM GCVE PQKS GR L Y QWTST A YTQWPICI E LV L -- N H G VPLW ygkqvcpdtg 477
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 457 ------ TYE GLLEQIRAK I SHYITLMVEGSN I CDIG HRD W AP V PL L S SFISD C L EKG R D ITD GGA R YNF S - GV QGI G I A N 529
Cdd:TIGR04394 478 dlsqfd TYE KFDAAVKEQ I KYITKWSAVATV I SQRV HRD L AP K PL M S LMYEG C M EKG K D VSA GGA M YNF G p GV VWS G L A T 557
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 530 LS DS LH A L K GM V F D QQRLSFDE L LSV LKANF atp EG - E KV RA RLINR f E KYGND I D EV D N I S A E L LRHYCK E VE KY QNPR 608
Cdd:TIGR04394 558 YA DS MA A I K KL V Y D DKKYTLEQ L NEA LKANF --- EG y E QI RA DCLDA - P KYGND D D YA D L I A A D L VNFTER E HR KY KTLY 633
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 609 g GYFTP G SYTV S AHV P L G SVV GA TPD GR F A GEQ L A D g G L SP ML G Q D AQ GPTA VL KSVSK LD N TLLSN G TLL N V K FTPAT L 688
Cdd:TIGR04394 634 - SHLSH G TLSI S NNT P F G QLT GA SAN GR L A WTP L S D - G I SP TQ G A D FK GPTA II KSVSK MA N DSMNI G MVH N F K LMSGL L 711
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1617439169 689 --- EGE A GL RK L adf LR AFTQ L KLQHI QFN VVNAD TL RE AQQ R P QD Y AG LVVRVAGYSAFFVEL S K EI QD D II R RT 761
Cdd:TIGR04394 712 dtp EGE N GL IT L --- LR TASI L GNGEM QFN YLDNE TL LD AQQ H P EK Y RD LVVRVAGYSAFFVEL C K DV QD E II S RT 784
PFL1
cd01678 Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ...
59-763
3.15e-40
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain]
Cd Length: 738
Bit Score: 158.30
E-value: 3.15e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 59 RDE E L I A G NR T V KP -- RA g IM spemd P YWLLKELD Q FPT rp QDRFAISE E D K R I YREE lfpywe KRSMK D FINGQM T D E V 136
Cdd:cd01678 82 KEL E V I V G LQ T D KP lk RA - IM ----- P FGGIRMAE Q ALK -- AYGYELDP E L K K I FTKY ------ RKTHN D GVFDAY T P E I 147
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 137 KA A TSTQ I FSINQTDK G Q G H II I DY P R LLNH G LGE L VAQMQQHCQQQPENHFYQAALL L L E ASQKH I LRYA EL AE t MAA N 216
Cdd:cd01678 148 RR A RHSG I ITGLPDAY G R G R II G DY R R VALY G VDR L IEEKKKDLDNLGGDEMTDDTIR L R E EVAEQ I KALK EL KQ - MAA S 226
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 217 CT daqrreellti AE ISR -- H NAQ HKP Q tf W Q acq L F W Y MNI I lq Y E S N ASSL SLGR FDQYMLPFYQTS L TQ G E - DA A FL 293
Cdd:cd01678 227 YG ----------- LD ISR pa T NAQ EAI Q -- W T --- Y F G Y LAA I -- K E Q N GAAM SLGR VSTFLDIYIERD L KA G T i TE A EA 288
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 294 K EL LESLWV K CNDIVL LR STSSARY F A G F PT GY T ALL GG LTEN GRS A V NVL SF LC L DA - Y QSVQL P Q PNL G V RTNALIDT 372
Cdd:cd01678 289 Q EL IDQFIM K LRMVRF LR TPEYNEL F S G D PT WV T ESI GG MGND GRS L V TKT SF RF L NT l Y NLGPA P E PNL T V LWSEKLPE 368
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 373 P F l MKTAETIRLG T GIP Q IF ND EVVV P AFLNR gvsledar DY SVVG CV ELSIP G R tyglh DIAM F ---- NL L K VMEICL h 448
Cdd:cd01678 369 N F - KRFCAKVSID T SSI Q YE ND DLMR P DWGGD -------- DY GIAC CV SAMRI G K ----- QMQF F gara NL A K ALLYAI - 433
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 449 e N E G NAAL T YEGLLEQ I RAKI S H Y ITLMVEGS N ICDI gh R DW apvpl L SSFISDC L EKGRDIT D gga R Y NFSGV Q ----- 523
Cdd:cd01678 434 - N G G RDEK T GDQVGPD I EPIT S D Y LDYDEVME N YDKS -- M DW ----- L ADTYVNA L NIIHYMH D --- K Y AYEAL Q malhd 502
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 524 ------- GI GIA N LS --- DSL H A L K ---- GMVF D QQR L SF D ellsvlkanf ATP EG E kvrarlinr F EK YGND I D EV D N I 589
Cdd:cd01678 503 tdvrrtm AF GIA G LS vaa DSL S A I K yakv KPIR D EDG L AV D ---------- FEI EG D --------- F PR YGND D D RA D D I 563
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 590 SAELLRHYCKEVE K YQNP R GGYF T PGSY T VSAH V PL G SVV G A TPDGR F AGE QL A D G G l S PM L G Q D AQ G PT A V L K SV S KL D 669
Cdd:cd01678 564 AVWVVKTFMNKLR K HKTY R NAEP T QSVL T ITSN V VY G KKT G N TPDGR R AGE PF A P G A - N PM H G R D KK G AL A S L A SV A KL P 642
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 670 NTLLSN G TLLNVKFT P AT L -- EG E AGLRK L ADF L RAFTQLKLQ H IQF NV V N AD TL RE A QQR P QD Y AG L VV RV A GY SAF FV 747
Cdd:cd01678 643 YRDAND G ISNTFSIV P NA L gk TD E ERIDN L VGI L DGYFTKGGH H LNV NV L N RE TL LD A MEH P EK Y PQ L TI RV S GY AVN FV 722
730
....*....|....*.
gi 1617439169 748 E L SK E I Q D D I I R RT A H 763
Cdd:cd01678 723 K L TR E Q Q L D V I S RT F H 738
Gly_radical
pfam01228 Glycine radical;
646-747
1.42e-36
Glycine radical;
Pssm-ID: 426140 [Multi-domain]
Cd Length: 106
Bit Score: 132.68
E-value: 1.42e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 646 G L SP ML G Q D AQ GPTAVL K SV S K L D NTLLSN G TL LN V KF T PA T L E -- G E A G LRK L ADFLRAFTQ l KLQ H I QFNVV NAD TL R 723
Cdd:pfam01228 4 G I SP SH G A D FE GPTAVL N SV G K I D YEVELD G IS LN Q KF L PA V L G yy D E E G YAN L NTLIDTYFE - GGH H L QFNVV DRE TL P 82
90 100
....*....|....*....|....
gi 1617439169 724 E AQ QR P QD Y AG L V VRV A GYSA F FV 747
Cdd:pfam01228 83 D AQ KH P EK Y PD L T VRV S GYSA N FV 106
RNR_PFL
cd00576 Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and ...
241-716
8.15e-14
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and pyruvate formate lyase (PFL) are believed to have diverged from a common ancestor. They have a structurally similar ten-stranded alpha-beta barrel domain that hosts the active site, and are radical enzymes. RNRs are found in all organisms and provide the only mechanism by which nucleotides are converted to deoxynucleotides. RNRs are separated into three classes based on their metallocofactor usage. Class I RNRs use a diiron-tyrosyl radical while Class II RNRs use coenzyme B12 (adenosylcobalamin, AdoCbl). Class III RNRs use an FeS cluster and S-adenosylmethionine to generate a glycyl radical. PFL, an essential enzyme in anaerobic bacteria, catalyzes the conversion of pyruvate and CoA to acteylCoA and formate in a mechanism that uses a glycyl radical.
Pssm-ID: 153083 [Multi-domain]
Cd Length: 401
Bit Score: 74.11
E-value: 8.15e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 241 PQTFWQ A C Q LFWYMNIILQYES N ASSL S LG R FDQYML P FYQTSLTQ G E - DAAFLK E LLESLWVKCNDIV llrstssary F 319
Cdd:cd00576 45 AKSINE A I Q KTYQIIALAASNQ N GGGV S FA R ASSILS P YGSRDYAK G S g TETDAV E AADAFNLALKEVG ---------- Q 114
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 320 AGFP TG YTALLG G LTEN G RSAVNVLS FL C L DAYQSVQ --- L PQ PNL G VR TNALIDTP ------ FLMKT AE TI R l G TG I P Q 390
Cdd:cd00576 115 GNGR TG AATGFI G GVHK G KGDKISQE FL N L ALANGGE gip L NF PNL S VR VSSDKPGI lvkave LKQLI AE EA R - K TG S P G 193
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 391 IFNDE vvvpaf L NRG VS ledardysvvgcvelsipgrtyglhdiam F NL LKV ME iclhenegnaa LTYE G LLEQIRAKIS 470
Cdd:cd00576 194 IFNDE ------ L CNL VS ----------------------------- L NL ARI ME ----------- KAIN G SMDVVLEELE 227
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 471 HYIT L M V EGSNICDIG H RDWA P vpllssfisdclekgr D I TD GG ARYNFS G VQGI G I A N L SDS L halkgmvfdqqrlsfd 550
Cdd:cd00576 228 ELAF L A V RALDCVIDS H DERI P ---------------- T I EL GG DERRTV G LGIA G V A D L LIK L ---------------- 275
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 551 ellsvlkanfatp EG EKV R arlinrfekygnd ID E V D NIS AEL LRHYC K EVE K YQ N P RG GY F TP G SYTVSA hvp LG S VVG 630
Cdd:cd00576 276 ------------- GL EKV G ------------- DP E A D DLA AEL VDQLK K HLV K AT N E RG FN F LL G LSPSES --- NS S GAP 326
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1617439169 631 AT P dgrfageqladg G L SP ML G Q ------ DAQ GP TAV L K SV SK L D n TLLS NG TLLNVKFTPATLEGE aglr K L ADFLRAF 704
Cdd:cd00576 327 AT N ------------ G V SP SR G * iaivln GDI GP EES L A SV AI L Q - FYAD NG ISDTITIPDSATNLD ---- Q L LAVIDGA 389
490
....*....|..
gi 1617439169 705 TQL K LQ H IQF N V 716
Cdd:cd00576 390 AAI K TT H VRV N P 401
PRK11127
PRK11127 autonomous glycyl radical cofactor GrcA; Provisional
709-764
7.62e-13
autonomous glycyl radical cofactor GrcA; Provisional
Pssm-ID: 182983 [Multi-domain]
Cd Length: 127
Bit Score: 66.02
E-value: 7.62e-13
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*.
gi 1617439169 709 L QH IQF NV VNAD TL RE A QQR P QD Y AG L VV RV A GY SAF F VE L SK E I Q D D I I R RT AHQ 764
Cdd:PRK11127 70 G QH LNV NV LRRE TL ED A VKH P EK Y PQ L TI RV S GY AVR F NS L TP E Q Q R D V I A RT FTE 125
Blast search parameters
Data Source:
Precalculated data, version = cdd.v.3.21
Preset Options: Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
