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Conserved domains on  [gi|612673656|gb|EZS89716|]
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hypothetical protein W470_00876 [Staphylococcus aureus VET0159R]

Protein Classification

acyl-CoA synthetase( domain architecture ID 11417514)

acyl-CoA synthetase catalyzes the ATP-dependent activation of fatty acids in a two-step reaction to form acyl-CoA esters

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
14-527 0e+00

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


:

Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 601.72  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  14 FAPEKYNIISEIEKySHI---PDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELY 90
Cdd:COG0365    3 FVGGRLNIAYNCLD-RHAegrGDKVALIWEGEDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAM 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  91 IAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIA---------LEPFTVEFEKI-KEYDALTKFVIAG------QKDG 154
Cdd:COG0365   82 LACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITadgglrggkVIDLKEKVDEAlEELPSLEHVIVVGrtgadvPMEG 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 155 WISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWV 234
Cdd:COG0365  162 DLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADIGWATGH 241
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 235 WSPFLSVLGSGATAFVFNGRF---SPETYLELLQKYQINVLCCTPTEYRMMAK--LQNLNDYDLTHLHSAVSAGEPLNRE 309
Cdd:COG0365  242 SYIVYGPLLNGATVVLYEGRPdfpDPGRLWELIEKYGVTVFFTAPTAIRALMKagDEPLKKYDLSSLRLLGSAGEPLNPE 321
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 310 VVEQFKKYFNLTVRDGYGQTEST-LLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGY 388
Cdd:COG0365  322 VWEWWYEAVGVPIVDGWGQTETGgIFISNLPGLPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGPWPGMFRGY 401
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 389 YKEPERTAKAQTGK---YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGN 465
Cdd:COG0365  402 WNDPERYRETYFGRfpgWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQ 481
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 612673656 466 IVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAE 527
Cdd:COG0365  482 VVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIA 543
 
Name Accession Description Interval E-value
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
14-527 0e+00

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 601.72  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  14 FAPEKYNIISEIEKySHI---PDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELY 90
Cdd:COG0365    3 FVGGRLNIAYNCLD-RHAegrGDKVALIWEGEDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAM 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  91 IAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIA---------LEPFTVEFEKI-KEYDALTKFVIAG------QKDG 154
Cdd:COG0365   82 LACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITadgglrggkVIDLKEKVDEAlEELPSLEHVIVVGrtgadvPMEG 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 155 WISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWV 234
Cdd:COG0365  162 DLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADIGWATGH 241
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 235 WSPFLSVLGSGATAFVFNGRF---SPETYLELLQKYQINVLCCTPTEYRMMAK--LQNLNDYDLTHLHSAVSAGEPLNRE 309
Cdd:COG0365  242 SYIVYGPLLNGATVVLYEGRPdfpDPGRLWELIEKYGVTVFFTAPTAIRALMKagDEPLKKYDLSSLRLLGSAGEPLNPE 321
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 310 VVEQFKKYFNLTVRDGYGQTEST-LLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGY 388
Cdd:COG0365  322 VWEWWYEAVGVPIVDGWGQTETGgIFISNLPGLPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGPWPGMFRGY 401
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 389 YKEPERTAKAQTGK---YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGN 465
Cdd:COG0365  402 WNDPERYRETYFGRfpgWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQ 481
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 612673656 466 IVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAE 527
Cdd:COG0365  482 VVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIA 543
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
49-525 0e+00

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 566.58  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  49 VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIale 128
Cdd:cd05972    1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIV--- 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 129 pftvefekikeydaltkfviagqkdgwisleaekenasnklemadTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQM 208
Cdd:cd05972   78 ---------------------------------------------TDAEDPALIYFTSGTTGLPKGVLHTHSYPLGHIPT 112
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 209 ApKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNG-RFSPETYLELLQKYQINVLCCTPTEYRMMAKlQN 287
Cdd:cd05972  113 A-AYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGpRFDAERILELLERYGVTSFCGPPTAYRMLIK-QD 190
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 288 LNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSV 367
Cdd:cd05972  191 LSSYKFSHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMPVKPGSMGRPTPGYDVAIIDDDGREL 270
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 368 PANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNH 447
Cdd:cd05972  271 PPGEEGDIAIKLPPPGLFLGYVGDPEKTEASIRGDYYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEH 350
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 612673656 448 PAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd05972  351 PAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
18-521 2.61e-142

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 422.38  E-value: 2.61e-142
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  18 KYNIISE-IEKYSHIPDK-KAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIatyELYIA--- 92
Cdd:PRK04319  41 KVNIAYEaIDRHADGGRKdKVALRYLDASRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIP---ELYFAllg 117
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  93 ALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIAlepfTVEFEKIKEYDALT--KFVI-----AGQKDGWISLEAEKENA 165
Cdd:PRK04319 118 ALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLIT----TPALLERKPADDLPslKHVLlvgedVEEGPGTLDFNALMEQA 193
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 166 SNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMApKHWLCINEDDLVWATAAPGW----QKWVWSPFLSv 241
Cdd:PRK04319 194 SDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHNAMLQHYQTG-KYVLDLHEDDVYWCTADPGWvtgtSYGIFAPWLN- 271
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 242 lgsGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKL--QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFN 319
Cdd:PRK04319 272 ---GATNVIDGGRFSPERWYRILEDYKVTVWYTAPTAIRMLMGAgdDLVKKYDLSSLRHILSVGEPLNPEVVRWGMKVFG 348
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 320 LTVRDGYGQTES-TLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKA 398
Cdd:PRK04319 349 LPIHDNWWMTETgGIMIANYPAMDIKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIKKGWPSMMRGIWNNPEKYESY 428
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 399 QTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTA 478
Cdd:PRK04319 429 FAGDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYE 508
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 612673656 479 GDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI-RRV 521
Cdd:PRK04319 509 PSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKImRRV 552
AMP-binding pfam00501
AMP-binding enzyme;
32-431 7.07e-88

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 276.89  E-value: 7.07e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   32 PDKKAILYhnteGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:pfam00501   9 PDKTALEV----GEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRLPAEE 84
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  112 LQYRITHGEINAVIALEPFTVE-FEKIKEYDALTKFVIAGQKDGWISLEAEKENASNK----LEMADTTRDDMAILSYTS 186
Cdd:pfam00501  85 LAYILEDSGAKVLITDDALKLEeLLEALGKLEVVKLVLVLDRDPVLKEEPLPEEAKPAdvppPPPPPPDPDDLAYIIYTS 164
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  187 GTTGNPKAVTHCHG---WGFAHLQMAPKHWLCINEDDLVWATA----APGWQKWVWSPFLsvlgSGATAFVFNG--RFSP 257
Cdd:pfam00501 165 GTTGKPKGVMLTHRnlvANVLSIKRVRPRGFGLGPDDRVLSTLplfhDFGLSLGLLGPLL----AGATVVLPPGfpALDP 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  258 ETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEST--LLI 335
Cdd:pfam00501 241 AALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSSLRLVLSGGAPLPPELARRFRELFGGALVNGYGLTETTgvVTT 320
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  336 GFLKDTPQR-IGSMGKGIPGSSVTVVDDE-GNSVPANTKGNIAVplDLPALFKGYYKEPERTAKA-QTGKYYITGDLAHV 412
Cdd:pfam00501 321 PLPLDEDLRsLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCV--RGPGVMKGYLNDPELTAEAfDEDGWYRTGDLGRR 398
                         410
                  ....*....|....*....
gi 612673656  413 DDDGYFWFEGRRDDIIISS 431
Cdd:pfam00501 399 DEDGYLEIVGRKKDQIKLG 417
benz_CoA_lig TIGR02262
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ...
48-526 9.45e-85

benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.


Pssm-ID: 274059 [Multi-domain]  Cd Length: 505  Bit Score: 271.71  E-value: 9.45e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIAL 127
Cdd:TIGR02262  30 SLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADDYAYMLEDSRARVVFVS 109
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  128 EPFTVEFEK-IKEYDALTKFVIAGQKD-GWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAH 205
Cdd:TIGR02262 110 GALLPVIKAaLGKSPHLEHRVVVGRPEaGEVQLAELLATESEQFKPAATQADDPAFWLYSSGSTGMPKGVVHTHSNPYWT 189
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  206 LQMAPKHWLCINEDDLVWATA----APGWQKWVWSPflsvLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRM 281
Cdd:TIGR02262 190 AELYARNTLGIREDDVCFSAAklffAYGLGNALTFP----MSVGATTVLMGERPTPDAVFDRLRRHQPTIFYGVPTLYAA 265
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  282 MAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEstLLIGFLKDTPQRI--GSMGKGIPGSSVTV 359
Cdd:TIGR02262 266 MLADPNLPSEDQVRLRLCTSAGEALPAEVGQRWQARFGVDIVDGIGSTE--MLHIFLSNLPGDVryGTSGKPVPGYRLRL 343
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  360 VDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFE 439
Cdd:TIGR02262 344 VGDGGQDVADGEPGELLI--SGPSSATMYWNNRAKSRDTFQGEWTRSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFE 421
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  440 VEDALTNHPAVKECAVVAKpHDIRGNIV-KAFVILQdhtAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI 518
Cdd:TIGR02262 422 IESALIQHPAVLEAAVVGV-ADEDGLIKpKAFVVLR---PGQTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKI 497

                  ....*...
gi 612673656  519 RRVELRDA 526
Cdd:TIGR02262 498 QRFKLREG 505
 
Name Accession Description Interval E-value
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
14-527 0e+00

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 601.72  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  14 FAPEKYNIISEIEKySHI---PDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELY 90
Cdd:COG0365    3 FVGGRLNIAYNCLD-RHAegrGDKVALIWEGEDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAM 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  91 IAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIA---------LEPFTVEFEKI-KEYDALTKFVIAG------QKDG 154
Cdd:COG0365   82 LACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITadgglrggkVIDLKEKVDEAlEELPSLEHVIVVGrtgadvPMEG 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 155 WISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWV 234
Cdd:COG0365  162 DLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADIGWATGH 241
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 235 WSPFLSVLGSGATAFVFNGRF---SPETYLELLQKYQINVLCCTPTEYRMMAK--LQNLNDYDLTHLHSAVSAGEPLNRE 309
Cdd:COG0365  242 SYIVYGPLLNGATVVLYEGRPdfpDPGRLWELIEKYGVTVFFTAPTAIRALMKagDEPLKKYDLSSLRLLGSAGEPLNPE 321
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 310 VVEQFKKYFNLTVRDGYGQTEST-LLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGY 388
Cdd:COG0365  322 VWEWWYEAVGVPIVDGWGQTETGgIFISNLPGLPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGPWPGMFRGY 401
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 389 YKEPERTAKAQTGK---YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGN 465
Cdd:COG0365  402 WNDPERYRETYFGRfpgWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQ 481
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 612673656 466 IVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAE 527
Cdd:COG0365  482 VVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIA 543
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
49-525 0e+00

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 566.58  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  49 VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIale 128
Cdd:cd05972    1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIV--- 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 129 pftvefekikeydaltkfviagqkdgwisleaekenasnklemadTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQM 208
Cdd:cd05972   78 ---------------------------------------------TDAEDPALIYFTSGTTGLPKGVLHTHSYPLGHIPT 112
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 209 ApKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNG-RFSPETYLELLQKYQINVLCCTPTEYRMMAKlQN 287
Cdd:cd05972  113 A-AYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGpRFDAERILELLERYGVTSFCGPPTAYRMLIK-QD 190
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 288 LNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSV 367
Cdd:cd05972  191 LSSYKFSHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMPVKPGSMGRPTPGYDVAIIDDDGREL 270
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 368 PANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNH 447
Cdd:cd05972  271 PPGEEGDIAIKLPPPGLFLGYVGDPEKTEASIRGDYYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEH 350
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 612673656 448 PAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd05972  351 PAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
15-525 3.13e-156

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 456.57  E-value: 3.13e-156
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  15 APEKYNIISEI-EKYSHI-PDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIA 92
Cdd:cd05970   12 VPENFNFAYDVvDAMAKEyPDKLALVWCDDAGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLA 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  93 ALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTV--EFEK-IKEYDALTKFVIAG--QKDGWISLEAEKENASN 167
Cdd:cd05970   92 LHKLGAIAIPATHQLTAKDIVYRIESADIKMIVAIAEDNIpeEIEKaAPECPSKPKLVWVGdpVPEGWIDFRKLIKNASP 171
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 168 KLEM----ADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMApKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLG 243
Cdd:cd05970  172 DFERptanSYPCGEDILLVYFSSGTTGMPKMVEHDFTYPLGHIVTA-KYWQNVREGGLHLTVADTGWGKAVWGKIYGQWI 250
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 244 SGATAFVFN-GRFSPETYLELLQKYQINVLCCTPTEYRMMAKlQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTV 322
Cdd:cd05970  251 AGAAVFVYDyDKFDPKALLEKLSKYGVTTFCAPPTIYRFLIR-EDLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIKL 329
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 323 RDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLD--LP-ALFKGYYKEPERTAKAQ 399
Cdd:cd05970  330 MEGFGQTETTLTIATFPWMEPKPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIRTSkgKPvGLFGGYYKDAEKTAEVW 409
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 400 TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVIL-QDHTA 478
Cdd:cd05970  410 HDGYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLaKGYEP 489
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 612673656 479 GdDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd05970  490 S-EELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIRE 535
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
16-527 3.98e-148

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 435.74  E-value: 3.98e-148
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  16 PEKYNIISEI-------EKYSHIPDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASH-GLSKGDKVLIMMPRSIATY 87
Cdd:cd05928    2 PEYFNFASDVldqwadkEKAGKRPPNPALWWVNGKGDEVKWSFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWW 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  88 ELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVEFEKI-KEYDAL-TKFVIAGQ-KDGWISLEAEKEN 164
Cdd:cd05928   82 LVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSDELAPEVDSVaSECPSLkTKLLVSEKsRDGWLNFKELLNE 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 165 ASNKLEMADT-TRDDMAILsYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLG 243
Cdd:cd05928  162 ASTEHHCVETgSQEPMAIY-FTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWI 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 244 SGATAFVFN-GRFSPETYLELLQKYQINVLCCTPTEYRMMAKlQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTV 322
Cdd:cd05928  241 QGACVFVHHlPRFDPLVILKTLSSYPITTFCGAPTVYRMLVQ-QDLSSYKFPSLQHCVTGGEPLNPEVLEKWKAQTGLDI 319
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 323 RDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAV---PLDLPALFKGYYKEPERTAKAQ 399
Cdd:cd05928  320 YEGYGQTETGLICANFKGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIrvkPIRPFGLFSGYVDNPEKTAATI 399
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 400 TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAG 479
Cdd:cd05928  400 RGDFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAFVVLAPQFLS 479
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|
gi 612673656 480 DD--TLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAE 527
Cdd:cd05928  480 HDpeQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRDKE 529
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
18-521 2.61e-142

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 422.38  E-value: 2.61e-142
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  18 KYNIISE-IEKYSHIPDK-KAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIatyELYIA--- 92
Cdd:PRK04319  41 KVNIAYEaIDRHADGGRKdKVALRYLDASRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIP---ELYFAllg 117
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  93 ALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIAlepfTVEFEKIKEYDALT--KFVI-----AGQKDGWISLEAEKENA 165
Cdd:PRK04319 118 ALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLIT----TPALLERKPADDLPslKHVLlvgedVEEGPGTLDFNALMEQA 193
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 166 SNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMApKHWLCINEDDLVWATAAPGW----QKWVWSPFLSv 241
Cdd:PRK04319 194 SDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHNAMLQHYQTG-KYVLDLHEDDVYWCTADPGWvtgtSYGIFAPWLN- 271
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 242 lgsGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKL--QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFN 319
Cdd:PRK04319 272 ---GATNVIDGGRFSPERWYRILEDYKVTVWYTAPTAIRMLMGAgdDLVKKYDLSSLRHILSVGEPLNPEVVRWGMKVFG 348
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 320 LTVRDGYGQTES-TLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKA 398
Cdd:PRK04319 349 LPIHDNWWMTETgGIMIANYPAMDIKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIKKGWPSMMRGIWNNPEKYESY 428
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 399 QTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTA 478
Cdd:PRK04319 429 FAGDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYE 508
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 612673656 479 GDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI-RRV 521
Cdd:PRK04319 509 PSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKImRRV 552
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
32-530 1.16e-136

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 403.42  E-value: 1.16e-136
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:COG0318   13 PDRPALVF-----GGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPRLTAEE 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIAlepftvefekikeydaltkfviagqkdgwisleaekenasnklemadttrddmAILSYTSGTTGN 191
Cdd:COG0318   88 LAYILEDSGARALVT-----------------------------------------------------ALILYTSGTTGR 114
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGwGFAHLQMAPKHWLCINEDDlVWATAAP-----GWqkwvWSPFLSVLGSGATAfVFNGRFSPETYLELLQK 266
Cdd:COG0318  115 PKGVMLTHR-NLLANAAAIAAALGLTPGD-VVLVALPlfhvfGL----TVGLLAPLLAGATL-VLLPRFDPERVLELIER 187
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 267 YQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLI--GFLKDTPQR 344
Cdd:COG0318  188 ERVTVLFGVPTMLARLLRHPEFARYDLSSLRLVVSGGAPLPPELLERFEERFGVRIVEGYGLTETSPVVtvNPEDPGERR 267
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 345 IGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRR 424
Cdd:COG0318  268 PGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVV--RGPNVMKGYWNDPEATAEAFRDGWLRTGDLGRLDEDGYLYIVGRK 345
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 425 DDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDdtlVKELQQFVKNEVAPYKYPREI 504
Cdd:COG0318  346 KDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAELD---AEELRAFLRERLARYKVPRRV 422
                        490       500
                 ....*....|....*....|....*.
gi 612673656 505 EFVDDLPKTNSGKIRRVELRDAEIEK 530
Cdd:COG0318  423 EFVDELPRTASGKIDRRALRERYAAG 448
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
50-528 2.79e-131

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 389.55  E-value: 2.79e-131
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEp 129
Cdd:cd05969    2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTE- 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 130 ftvefekikeydaltkfviagqkdgwisleaekenasnklEMAD-TTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQM 208
Cdd:cd05969   81 ----------------------------------------ELYErTDPEDPTLLHYTSGTTGTPKGVLHVHDAMIFYYFT 120
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 209 APKHwLCINEDDLVWATAAPGWQK----WVWSPFLSvlgsGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAK 284
Cdd:cd05969  121 GKYV-LDLHPDDIYWCTADPGWVTgtvyGIWAPWLN----GVTNVVYEGRFDAESWYGIIERVKVTVWYTAPTAIRMLMK 195
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 285 L--QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTES-TLLIGFLKDTPQRIGSMGKGIPGSSVTVVD 361
Cdd:cd05969  196 EgdELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFGVPIHDTWWQTETgSIMIANYPCMPIKPGSMGKPLPGVKAAVVD 275
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 362 DEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVE 441
Cdd:cd05969  276 ENGNELPPGTKGILALKPGWPSMFRGIWNDEERYKNSFIDGWYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVE 355
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 442 DALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRV 521
Cdd:cd05969  356 SALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRR 435

                 ....*..
gi 612673656 522 ELRDAEI 528
Cdd:cd05969  436 VLKAKEL 442
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
49-525 1.51e-115

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 349.04  E-value: 1.51e-115
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  49 VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALE 128
Cdd:cd05971    7 VTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVTDG 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 129 PftvefekikeydaltkfviagqkdgwisleaekenasnklemadttrDDMAILSYTSGTTGNPKAVTHCHGWGFAHL-- 206
Cdd:cd05971   87 S-----------------------------------------------DDPALIIYTSGTTGPPKGALHAHRVLLGHLpg 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 207 -QMApkHWLCINEDDLVWATAapgwqKWVWspfLSVLGSGATAFVFNG---------RFSPETYLELLQKYQINVLCCTP 276
Cdd:cd05971  120 vQFP--FNLFPRDGDLYWTPA-----DWAW---IGGLLDVLLPSLYFGvpvlahrmtKFDPKAALDLMSRYGVTTAFLPP 189
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 277 TEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT-PQRIGSMGKGIPGS 355
Cdd:cd05971  190 TALKMMRQQGEQLKHAQVKLRAIATGGESLGEELLGWAREQFGVEVNEFYGQTECNLVIGNCSALfPIKPGSMGKPIPGH 269
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 356 SVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTI 435
Cdd:cd05971  270 RVAIVDDNGTPLPPGEVGEIAVELPDPVAFLGYWNNPSATEKKMAGDWLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRI 349
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 436 GPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS 515
Cdd:cd05971  350 GPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDALAREIQELVKTRLAAHEYPREIEFVNELPRTAT 429
                        490
                 ....*....|
gi 612673656 516 GKIRRVELRD 525
Cdd:cd05971  430 GKIRRRELRA 439
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
49-527 5.11e-115

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 347.63  E-value: 5.11e-115
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  49 VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGeinaviale 128
Cdd:cd05974    1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRVDRG--------- 71
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 129 pftvefekikeydaltKFVIAGQKDgwisleaekenasnklemaDTTRDDMAILSYTSGTTGNPKAVTHCH-GWGFAHLq 207
Cdd:cd05974   72 ----------------GAVYAAVDE-------------------NTHADDPMLLYFTSGTTSKPKLVEHTHrSYPVGHL- 115
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 208 mAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFN-GRFSPETYLELLQKYQINVLCCTPTEYRMMAKlQ 286
Cdd:cd05974  116 -STMYWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLFNyARFDAKRVLAALVRYGVTTLCAPPTVWRMLIQ-Q 193
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 287 NLNDYDlTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNS 366
Cdd:cd05974  194 DLASFD-VKLREVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQTETTALVGNSPGQPVKAGSMGRPLPGYRVALLDPDGAP 272
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 367 VpanTKGNIAVPL--DLPA-LFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDA 443
Cdd:cd05974  273 A---TEGEVALDLgdTRPVgLMKGYAGDPDKTAHAMRGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESV 349
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 444 LTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVdDLPKTNSGKIRRVEL 523
Cdd:cd05974  350 LIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPETALEIFRFSRERLAPYKRIRRLEFA-ELPKTISGKIRRVEL 428

                 ....
gi 612673656 524 RDAE 527
Cdd:cd05974  429 RRRE 432
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
17-524 2.09e-105

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 325.48  E-value: 2.09e-105
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  17 EKYNIISEI--EKYSHIPDKKAILYHNTegediSVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAAL 94
Cdd:cd05959    1 EKYNAATLVdlNLNEGRGDKTAFIDDAG-----SLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAI 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  95 KLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVI-----AGQKDGWISLEAEKENASNKL 169
Cdd:cd05959   76 RAGIVPVPVNTLLTPDDYAYYLEDSRARVVVVSGELAPVLAAALTKSEHTLVVLivsggAGPEAGALLLAELVAAEAEQL 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 170 EMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATA----APGWQKWVWSPFlsvlGSG 245
Cdd:cd05959  156 KPAATHADDPAFWLYSSGSTGRPKGVVHLHADIYWTAELYARNVLGIREDDVCFSAAklffAYGLGNSLTFPL----SVG 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 246 ATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDG 325
Cdd:cd05959  232 ATTVLMPERPTPAAVFKRIRRYRPTVFFGVPTLYAAMLAAPNLPSRDLSSLRLCVSAGEALPAEVGERWKARFGLDILDG 311
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 326 YGQTEstLLIGFLKDTPQRI--GSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKY 403
Cdd:cd05959  312 IGSTE--MLHIFLSNRPGRVryGTTGKPVPGYEVELRDEDGGDVADGEPGELYV--RGPSSATMYWNNRDKTRDTFQGEW 387
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTL 483
Cdd:cd05959  388 TRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRPGYEDSEAL 467
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 612673656 484 VKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd05959  468 EEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
MACS_like_2 cd05973
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
50-524 1.46e-104

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341277 [Multi-domain]  Cd Length: 437  Bit Score: 320.62  E-value: 1.46e-104
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcsemLRTkdlqyrithgeinaviALEP 129
Cdd:cd05973    2 TFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQP----LFT----------------AFGP 61
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 130 FTVEfEKIKEYDAltKFVIAgqkdgwisleaekeNASNKLEMADttrdDMAILSYTSGTTGNPKAVTH----CHGWGfAH 205
Cdd:cd05973   62 KAIE-HRLRTSGA--RLVVT--------------DAANRHKLDS----DPFVMMFTSGTTGLPKGVPVplraLAAFG-AY 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 206 LQMApkhwLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRM-MAK 284
Cdd:cd05973  120 LRDA----VDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEGGFSVESTWRVIERLGVTNLAGSPTAYRLlMAA 195
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 285 LQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIG--FLKDTPQRIGSMGKGIPGSSVTVVDD 362
Cdd:cd05973  196 GAEVPARPKGRLRRVSSAGEPLTPEVIRWFDAALGVPIHDHYGQTELGMVLAnhHALEHPVHAGSAGRAMPGWRVAVLDD 275
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 363 EGNSVPANTKGNIAVPLDLPAL--FKGYYKEPERTAkaqTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEV 440
Cdd:cd05973  276 DGDELGPGEPGRLAIDIANSPLmwFRGYQLPDTPAI---DGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDV 352
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 441 EDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:cd05973  353 ESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQR 432

                 ....
gi 612673656 521 VELR 524
Cdd:cd05973  433 FLLR 436
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
32-524 3.39e-103

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 318.35  E-value: 3.39e-103
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHntegeDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd05936   13 PDKTALIFM-----GRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPLYTPRE 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFTvefekikeyDALtkfviagqkdgwisleaekENASNKLEMADTTRDDMAILSYTSGTTGN 191
Cdd:cd05936   88 LEHILNDSGAKALIVAVSFT---------DLL-------------------AAGAPLGERVALTPEDVAVLQYTSGTTGV 139
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGWGFAHLQMApKHWL--CINEDDLVWATAapgwqkwvwsPFLSVLGSGA--TAFVFNG-------RFSPETY 260
Cdd:cd05936  140 PKGAMLTHRNLVANALQI-KAWLedLLEGDDVVLAAL----------PLFHVFGLTValLLPLALGativlipRFRPIGV 208
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 261 LELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGF-LK 339
Cdd:cd05936  209 LKEIRKHRVTIFPGVPTMYIALLNAPEFKKRDFSSLRLCISGGAPLPVEVAERFEELTGVPIVEGYGLTETSPVVAVnPL 288
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 340 DTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFW 419
Cdd:cd05936  289 DGPRKPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWV--RGPQVMKGYWNRPEETAEAFVDGWLRTGDIGYMDEDGYFF 366
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 420 FEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVKELQQFVKNEVAPYK 499
Cdd:cd05936  367 IVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKE---GASLTEEEIIAFCREQLAGYK 443
                        490       500
                 ....*....|....*....|....*
gi 612673656 500 YPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd05936  444 VPRQVEFRDELPKSAVGKILRRELR 468
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
178-519 1.52e-102

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 311.91  E-value: 1.52e-102
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 178 DMAILSYTSGTTGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPGWQkWVWSPFLSVLGSGATAFVFnGRFSP 257
Cdd:cd04433    1 DPALILYTSGTTGKPKGVVLSHR-NLLAAAAALAASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLL-PKFDP 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 258 ETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTE--STLLI 335
Cdd:cd04433   78 EAALELIEREKVTILLGVPTLLARLLKAPESAGYDLSSLRALVSGGAPLPPELLERFEEAPGIKLVNGYGLTEtgGTVAT 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 336 GFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDD 415
Cdd:cd04433  158 GPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVV--RGPSVMKGYWNNPEATAAVDEDGWYRTGDLGRLDED 235
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 416 GYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVKELQQFVKNEV 495
Cdd:cd04433  236 GYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRP---GADLDAEELRAHVRERL 312
                        330       340
                 ....*....|....*....|....
gi 612673656 496 APYKYPREIEFVDDLPKTNSGKIR 519
Cdd:cd04433  313 APYKVPRRVVFVDALPRTASGKID 336
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
46-524 1.59e-100

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 310.56  E-value: 1.59e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  46 DISVTYQQLIEQSNKVGNVL-ASHGLSKGDKVLIMMPRSIATYELYIAALKLG---VAIIPcseMLRTKDLQYRITHGEI 121
Cdd:cd05958    8 EREWTYRDLLALANRIANVLvGELGIVPGNRVLLRGSNSPELVACWFGIQKAGaiaVATMP---LLRPKELAYILDKARI 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 122 navialepftvefekikeydalTKFVIAGQKdgwisleaekenasnklemadTTRDDMAILSYTSGTTGNPKAVTHCHGW 201
Cdd:cd05958   85 ----------------------TVALCAHAL---------------------TASDDICILAFTSGTTGAPKATMHFHRD 121
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 202 GFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNGRfSPETYLELLQKYQINVLCCTPTEYRM 281
Cdd:cd05958  122 PLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEA-TPDLLLSAIARYKPTVLFTAPTAYRA 200
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 282 MAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEstLLIGFLKDTPQ--RIGSMGKGIPGSSVTV 359
Cdd:cd05958  201 MLAHPDAAGPDLSSLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTE--MFHIFISARPGdaRPGATGKPVPGYEAKV 278
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 360 VDDEGNSVPANTKGNIAVpldlpalfKG----YYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTI 435
Cdd:cd05958  279 VDDEGNPVPDGTIGRLAV--------RGptgcRYLADKRQRTYVQGGWNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNI 350
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 436 GPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS 515
Cdd:cd05958  351 APPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLARELQDHAKAHIAPYKYPRAIEFVTELPRTAT 430

                 ....*....
gi 612673656 516 GKIRRVELR 524
Cdd:cd05958  431 GKLQRFALR 439
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
46-524 1.02e-98

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 305.54  E-value: 1.02e-98
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  46 DISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVI 125
Cdd:cd05919    8 DRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVV 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 126 alepftvefekikeydaltkfviagqkdgwisleaekenasnklemadTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAH 205
Cdd:cd05919   88 ------------------------------------------------TSADDIAYLLYSSGTTGPPKGVMHAHRDPLLF 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 206 LQMAPKHWLCINEDDLVWATA----APGWQKWVWSPflsvLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRM 281
Cdd:cd05919  120 ADAMAREALGLTPGDRVFSSAkmffGYGLGNSLWFP----LAVGASAVLNPGWPTAERVLATLARFRPTVLYGVPTFYAN 195
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 282 MAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLigFLKDTPQ--RIGSMGKGIPGSSVTV 359
Cdd:cd05919  196 LLDSCAGSPDALRSLRLCVSAGEALPRGLGERWMEHFGGPILDGIGATEVGHI--FLSNRPGawRLGSTGRPVPGYEIRL 273
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 360 VDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFE 439
Cdd:cd05919  274 VDEEGHTIPPGEEGDLLV--RGPSAAVGYWNNPEKSRATFNGGWYRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVE 351
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 440 VEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIR 519
Cdd:cd05919  352 VESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLARDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQ 431

                 ....*
gi 612673656 520 RVELR 524
Cdd:cd05919  432 RFKLR 436
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
32-525 3.64e-97

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 304.42  E-value: 3.64e-97
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK06187  20 PDKEAVYF-----DGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPINIRLKPEE 94
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVI-------AGQKDGWISLEAEKENASNKLEMADTTRDDMAILSY 184
Cdd:PRK06187  95 IAYILNDAEDRVVLVDSEFVPLLAAILPQLPTVRTVIvegdgpaAPLAPEVGEYEELLAAASDTFDFPDIDENDAAAMLY 174
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 185 TSGTTGNPKAVTHCHGWGFAHLqMAPKHWLCINEDD--LVWA----TAAPGWqkwvwsPFLSVLgSGATAfVFNGRFSPE 258
Cdd:PRK06187 175 TSGTTGHPKGVVLSHRNLFLHS-LAVCAWLKLSRDDvyLVIVpmfhVHAWGL------PYLALM-AGAKQ-VIPRRFDPE 245
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 259 TYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFL 338
Cdd:PRK06187 246 NLLDLIETERVTFFFAVPTIWQMLLKAPRAYFVDFSSLRLVIYGGAALPPALLREFKEKFGIDLVQGYGMTETSPVVSVL 325
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 339 KDTPQ------RIGSMGKGIPGSSVTVVDDEGNSVPANTK--GNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLA 410
Cdd:PRK06187 326 PPEDQlpgqwtKRRSAGRPLPGVEARIVDDDGDELPPDGGevGEIIV--RGPWLMQGYWNRPEATAETIDGGWLHTGDVG 403
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 411 HVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVKELQQF 490
Cdd:PRK06187 404 YIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKP---GATLDAKELRAF 480
                        490       500       510
                 ....*....|....*....|....*....|....*
gi 612673656 491 VKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK06187 481 LRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLRE 515
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
29-520 1.84e-94

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 294.52  E-value: 1.84e-94
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  29 SHIPDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcsemlr 108
Cdd:cd17631    6 RRHPDRTALVF-----GGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVP------ 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 tkdLQYRITHGEINAVIAlepftvefekikeyDALTKFVIagqkdgwisleaekenasnklemadttrDDMAILSYTSGT 188
Cdd:cd17631   75 ---LNFRLTPPEVAYILA--------------DSGAKVLF----------------------------DDLALLMYTSGT 109
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 189 TGNPKAVTHCHGWGFAHLQMAPKHWlCINEDDlVWATAAP-----GWQKWVwspfLSVLGSGATAfVFNGRFSPETYLEL 263
Cdd:cd17631  110 TGRPKGAMLTHRNLLWNAVNALAAL-DLGPDD-VLLVVAPlfhigGLGVFT----LPTLLRGGTV-VILRKFDPETVLDL 182
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 264 LQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKyFNLTVRDGYGQTESTLLIGFL--KDT 341
Cdd:cd17631  183 IERHRVTSFFLVPTMIQALLQHPRFATTDLSSLRAVIYGGAPMPERLLRALQA-RGVKFVQGYGMTETSPGVTFLspEDH 261
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 342 PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFE 421
Cdd:cd17631  262 RRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVV--RGPHVMAGYWNRPEATAAAFRDGWFHTGDLGRLDEDGYLYIV 339
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 422 GRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTlvkELQQFVKNEVAPYKYP 501
Cdd:cd17631  340 DRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAELDED---ELIAHCRERLARYKIP 416
                        490
                 ....*....|....*....
gi 612673656 502 REIEFVDDLPKTNSGKIRR 520
Cdd:cd17631  417 KSVEFVDALPRNATGKILK 435
AMP-binding pfam00501
AMP-binding enzyme;
32-431 7.07e-88

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 276.89  E-value: 7.07e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   32 PDKKAILYhnteGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:pfam00501   9 PDKTALEV----GEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRLPAEE 84
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  112 LQYRITHGEINAVIALEPFTVE-FEKIKEYDALTKFVIAGQKDGWISLEAEKENASNK----LEMADTTRDDMAILSYTS 186
Cdd:pfam00501  85 LAYILEDSGAKVLITDDALKLEeLLEALGKLEVVKLVLVLDRDPVLKEEPLPEEAKPAdvppPPPPPPDPDDLAYIIYTS 164
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  187 GTTGNPKAVTHCHG---WGFAHLQMAPKHWLCINEDDLVWATA----APGWQKWVWSPFLsvlgSGATAFVFNG--RFSP 257
Cdd:pfam00501 165 GTTGKPKGVMLTHRnlvANVLSIKRVRPRGFGLGPDDRVLSTLplfhDFGLSLGLLGPLL----AGATVVLPPGfpALDP 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  258 ETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEST--LLI 335
Cdd:pfam00501 241 AALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSSLRLVLSGGAPLPPELARRFRELFGGALVNGYGLTETTgvVTT 320
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  336 GFLKDTPQR-IGSMGKGIPGSSVTVVDDE-GNSVPANTKGNIAVplDLPALFKGYYKEPERTAKA-QTGKYYITGDLAHV 412
Cdd:pfam00501 321 PLPLDEDLRsLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCV--RGPGVMKGYLNDPELTAEAfDEDGWYRTGDLGRR 398
                         410
                  ....*....|....*....
gi 612673656  413 DDDGYFWFEGRRDDIIISS 431
Cdd:pfam00501 399 DEDGYLEIVGRKKDQIKLG 417
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
32-525 1.01e-86

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 279.83  E-value: 1.01e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntEGEDIS----VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIatyELYIAAL---KLGVA----- 99
Cdd:cd05966   67 GDKVAIIW---EGDEPDqsrtITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIP---ELVIAMLacaRIGAVhsvvf 140
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 100 -----------IIPC-SEMLRTKDLQYR----ITHGEInAVIALE-PFTVE----FEKIKeydalTKFVIAGQKDGWISL 158
Cdd:cd05966  141 agfsaesladrINDAqCKLVITADGGYRggkvIPLKEI-VDEALEkCPSVEkvlvVKRTG-----GEVPMTEGRDLWWHD 214
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 159 EAEKENASNKLEMADTtrDDMAILSYTSGTTGNPKAVTHCHGwGFA-HLQMAPKHWLCINEDDLVWATAAPGW----QKW 233
Cdd:cd05966  215 LMAKQSPECEPEWMDS--EDPLFILYTSGSTGKPKGVVHTTG-GYLlYAATTFKYVFDYHPDDIYWCTADIGWitghSYI 291
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 234 VWSPflsvLGSGATAFVFNGRFS---PETYLELLQKYQINVLCCTPTEYRMMAKLQN--LNDYDLTHLHSAVSAGEPLNR 308
Cdd:cd05966  292 VYGP----LANGATTVMFEGTPTypdPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDewVKKHDLSSLRVLGSVGEPINP 367
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 309 EVVEQFKKY---FNLTVRDGYGQTES-----TLLIGFlkdTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLD 380
Cdd:cd05966  368 EAWMWYYEVigkERCPIVDTWWQTETggimiTPLPGA---TPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRP 444
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 381 LPALFKGYYKEPERTAKAQTGK---YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA 457
Cdd:cd05966  445 WPGMARTIYGDHERYEDTYFSKfpgYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVG 524
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 612673656 458 KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI-RRVeLRD 525
Cdd:cd05966  525 RPHDIKGEAIYAFVTLKDGEEPSDELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKImRRI-LRK 592
benz_CoA_lig TIGR02262
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ...
48-526 9.45e-85

benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.


Pssm-ID: 274059 [Multi-domain]  Cd Length: 505  Bit Score: 271.71  E-value: 9.45e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIAL 127
Cdd:TIGR02262  30 SLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADDYAYMLEDSRARVVFVS 109
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  128 EPFTVEFEK-IKEYDALTKFVIAGQKD-GWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAH 205
Cdd:TIGR02262 110 GALLPVIKAaLGKSPHLEHRVVVGRPEaGEVQLAELLATESEQFKPAATQADDPAFWLYSSGSTGMPKGVVHTHSNPYWT 189
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  206 LQMAPKHWLCINEDDLVWATA----APGWQKWVWSPflsvLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRM 281
Cdd:TIGR02262 190 AELYARNTLGIREDDVCFSAAklffAYGLGNALTFP----MSVGATTVLMGERPTPDAVFDRLRRHQPTIFYGVPTLYAA 265
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  282 MAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEstLLIGFLKDTPQRI--GSMGKGIPGSSVTV 359
Cdd:TIGR02262 266 MLADPNLPSEDQVRLRLCTSAGEALPAEVGQRWQARFGVDIVDGIGSTE--MLHIFLSNLPGDVryGTSGKPVPGYRLRL 343
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  360 VDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFE 439
Cdd:TIGR02262 344 VGDGGQDVADGEPGELLI--SGPSSATMYWNNRAKSRDTFQGEWTRSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFE 421
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  440 VEDALTNHPAVKECAVVAKpHDIRGNIV-KAFVILQdhtAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI 518
Cdd:TIGR02262 422 IESALIQHPAVLEAAVVGV-ADEDGLIKpKAFVVLR---PGQTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKI 497

                  ....*...
gi 612673656  519 RRVELRDA 526
Cdd:TIGR02262 498 QRFKLREG 505
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
50-524 3.14e-84

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 267.62  E-value: 3.14e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIAlep 129
Cdd:cd05934    5 TYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV--- 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 130 ftvefekikeydaltkfviagqkdgwisleaekenasnklemadttrdDMAILSYTSGTTGNPKAV--THCHgWGFAHLQ 207
Cdd:cd05934   82 ------------------------------------------------DPASILYTSGTTGPPKGVviTHAN-LTFAGYY 112
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 208 MApkHWLCINEDDlVWATAAP----GWQKWVWSPFLSVlgsGATaFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA 283
Cdd:cd05934  113 SA--RRFGLGEDD-VYLTVLPlfhiNAQAVSVLAALSV---GAT-LVLLPRFSASRFWSDVRRYGATVTNYLGAMLSYLL 185
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 284 KlQNLNDYDLTH-LHSAVSAGEPlnREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDD 362
Cdd:cd05934  186 A-QPPSPDDRAHrLRAAYGAPNP--PELHEEFEERFGVRLLEGYGMTETIVGVIGPRDEPRRPGSIGRPAPGYEVRIVDD 262
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 363 EGNSVPANTKGNIAV-PLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVE 441
Cdd:cd05934  263 DGQELPAGEPGELVIrGLRGWGFFKGYYNMPEATAEAMRNGWFHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVE 342
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 442 DALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRV 521
Cdd:cd05934  343 RAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRP---GETLDPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKA 419

                 ...
gi 612673656 522 ELR 524
Cdd:cd05934  420 QLR 422
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
48-518 9.55e-81

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 260.61  E-value: 9.55e-81
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIAL 127
Cdd:cd05911   10 ELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFTD 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 128 EPFtveFEKIKEydALTK-------FVIAGQKDGWISLE-----AEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAV 195
Cdd:cd05911   90 PDG---LEKVKE--AAKElgpkdkiIVLDDKPDGVLSIEdllspTLGEEDEDLPPPLKDGKDDTAAILYSSGTTGLPKGV 164
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 196 --THcHGWGFAHLQMAPKHWLCINEDD-------LVWATAAPGWqkwVWSPFLsvlgsGATAFVFNgRFSPETYLELLQK 266
Cdd:cd05911  165 clSH-RNLIANLSQVQTFLYGNDGSNDvilgflpLYHIYGLFTT---LASLLN-----GATVIIMP-KFDSELFLDLIEK 234
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 267 YQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTESTLLIGFLKDTPQRI 345
Cdd:cd05911  235 YKITFLYLVPPIAAALAKSPLLDKYDLSSLRVILSGGAPLSKELQELLAKRFpNATIKQGYGMTETGGILTVNPDGDDKP 314
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 346 GSMGKGIPGSSVTVVDDEGNS-VPANTKGNIAVplDLPALFKGYYKEPERTAKAQT-GKYYITGDLAHVDDDGYFWFEGR 423
Cdd:cd05911  315 GSVGRLLPNVEAKIVDDDGKDsLGPNEPGEICV--RGPQVMKGYYNNPEATKETFDeDGWLHTGDIGYFDEDGYLYIVDR 392
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 424 RDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVKELQQFVKNEVAPYKYPR- 502
Cdd:cd05911  393 KKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKP---GEKLTEKEVKDYVAKKVASYKQLRg 469
                        490
                 ....*....|....*.
gi 612673656 503 EIEFVDDLPKTNSGKI 518
Cdd:cd05911  470 GVVFVDEIPKSASGKI 485
ACS-like cd17634
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ...
32-518 2.88e-78

acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341289 [Multi-domain]  Cd Length: 587  Bit Score: 257.12  E-value: 2.88e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEGEDI-SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTK 110
Cdd:cd17634   67 GDRTAIIYEGDDTSQSrTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPE 146
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 111 DLQYRITHGEINAVIALEPF-----TVEFEKIKEyDAL---------------TKFVIAGQKDGWISLEAEKENASNKLE 170
Cdd:cd17634  147 AVAGRIIDSSSRLLITADGGvragrSVPLKKNVD-DALnpnvtsvehvivlkrTGSDIDWQEGRDLWWRDLIAKASPEHQ 225
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 171 MADTTRDDMAILSYTSGTTGNPKAVTHCHGwGFA-HLQMAPKHWLCINEDDLVWATAAPGWQkwVWSPFL--SVLGSGAT 247
Cdd:cd17634  226 PEAMNAEDPLFILYTSGTTGKPKGVLHTTG-GYLvYAATTMKYVFDYGPGDIYWCTADVGWV--TGHSYLlyGPLACGAT 302
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 248 AFVFNGRF---SPETYLELLQKYQINVLCCTPTEYRMMAKLQN--LNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLT- 321
Cdd:cd17634  303 TLLYEGVPnwpTPARMWQVVDKHGVNILYTAPTAIRALMAAGDdaIEGTDRSSLRILGSVGEPINPEAYEWYWKKIGKEk 382
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 322 --VRDGYGQTES-----TLLIGFLkdtPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPER 394
Cdd:cd17634  383 cpVVDTWWQTETggfmiTPLPGAI---ELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQTRTLFGDHER 459
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 395 ---TAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFV 471
Cdd:cd17634  460 feqTYFSTFKGMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYV 539
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 612673656 472 ILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI 518
Cdd:cd17634  540 VLNHGVEPSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKI 586
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
32-530 7.27e-78

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 254.88  E-value: 7.27e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEgedisVTYQQLIEQSNKVGNVLASH-GLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTK 110
Cdd:PRK08314  24 PDKTAIVFYGRA-----ISYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREE 98
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 111 DLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDG-----------WISLEAEKENASNKLEMA------- 172
Cdd:PRK08314  99 ELAHYVTDSGARVAIVGSELAPKVAPAVGNLRLRHVIVAQYSDYlpaepeiavpaWLRAEPPLQALAPGGVVAwkealaa 178
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 173 -------DTTRDDMAILSYTSGTTGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAapgwqkwvwsPFLSVLG-- 243
Cdd:PRK08314 179 glappphTAGPDDLAVLPYTSGTTGVPKGCMHTHR-TVMANAVGSVLWSNSTPESVVLAVL----------PLFHVTGmv 247
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 244 --------SGATaFVFNGRFSPETYLELLQKYQINVLCCTPTeyrMMAKL---QNLNDYDLTHLHSAVSAGEPLNREVVE 312
Cdd:PRK08314 248 hsmnapiyAGAT-VVLMPRWDREAAARLIERYRVTHWTNIPT---MVVDFlasPGLAERDLSSLRYIGGGGAAMPEAVAE 323
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 313 QFKKYFNLTVRDGYGQTEStllIGFLKDTP-QRIGSMGKGIPGSSVT--VVD-DEGNSVPANTKGNIAVplDLPALFKGY 388
Cdd:PRK08314 324 RLKELTGLDYVEGYGLTET---MAQTHSNPpDRPKLQCLGIPTFGVDarVIDpETLEELPPGEVGEIVV--HGPQVFKGY 398
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 389 YKEPERTAKAQT---GK-YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRG 464
Cdd:PRK08314 399 WNRPEATAEAFIeidGKrFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEACVIATPDPRRG 478
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 612673656 465 NIVKAFVILQDHTAGdDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEK 530
Cdd:PRK08314 479 ETVKAVVVLRPEARG-KTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQLQEQEKAR 543
PRK00174 PRK00174
acetyl-CoA synthetase; Provisional
33-525 7.17e-77

acetyl-CoA synthetase; Provisional


Pssm-ID: 234677 [Multi-domain]  Cd Length: 637  Bit Score: 254.68  E-value: 7.17e-77
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  33 DKKAILYhntEGEDIS----VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIatyELYIAAL---KLGVA------ 99
Cdd:PRK00174  82 DKVAIIW---EGDDPGdsrkITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIP---EAAVAMLacaRIGAVhsvvfg 155
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 100 ----------IIPC-SEMLRTKDLQYRithGeiNAVIALEPfTVEfEKIKEYDALTKfVI----AGQK-------DGWIS 157
Cdd:PRK00174 156 gfsaealadrIIDAgAKLVITADEGVR---G--GKPIPLKA-NVD-EALANCPSVEK-VIvvrrTGGDvdwvegrDLWWH 227
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 158 LEAEKENASNKLEMADTtrDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGW----QKW 233
Cdd:PRK00174 228 ELVAGASDECEPEPMDA--EDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWvtghSYI 305
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 234 VWSPflsvLGSGATAFVF--------NGRFSpetylELLQKYQINVLCCTPTEYRMMAKL--QNLNDYDLTHLHSAVSAG 303
Cdd:PRK00174 306 VYGP----LANGATTLMFegvpnypdPGRFW-----EVIDKHKVTIFYTAPTAIRALMKEgdEHPKKYDLSSLRLLGSVG 376
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 304 EPLNREVVEQFKKYF---NLTVRDGYGQTES-----TLLIGFlkdTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNI 375
Cdd:PRK00174 377 EPINPEAWEWYYKVVggeRCPIVDTWWQTETggimiTPLPGA---TPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNL 453
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 376 AVPLDLPALFKGYYKEPERTAKAQTGKY---YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKE 452
Cdd:PRK00174 454 VIKDPWPGMMRTIYGDHERFVKTYFSTFkgmYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAE 533
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 612673656 453 CAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI-RRVeLRD 525
Cdd:PRK00174 534 AAVVGRPDDIKGQGIYAFVTLKGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKImRRI-LRK 606
PrpE cd05967
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ...
32-531 1.64e-75

Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.


Pssm-ID: 341271 [Multi-domain]  Cd Length: 617  Bit Score: 250.31  E-value: 1.64e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTE-GEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGvAIipCSEML--- 107
Cdd:cd05967   65 GDQIALIYDSPVtGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIG-AI--HSVVFggf 141
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 108 RTKDLQYRITHGEINAVIALEpFTVEFEKIKEY-----DALT------KFVIAGQKDgwiSLEAEKENASNKL----EMA 172
Cdd:cd05967  142 AAKELASRIDDAKPKLIVTAS-CGIEPGKVVPYkplldKALElsghkpHHVLVLNRP---QVPADLTKPGRDLdwseLLA 217
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 173 DTTRDDMA---------ILsYTSGTTGNPKAVTHCHGwGFA-HLQMAPKHWLCINEDDLVWATAAPGW----QKWVWSPF 238
Cdd:cd05967  218 KAEPVDCVpvaatdplyIL-YTSGTTGKPKGVVRDNG-GHAvALNWSMRNIYGIKPGDVWWAASDVGWvvghSYIVYGPL 295
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 239 LSvlgsGATAFVFNGRFS----PETYLELLQKYQINVLCCTPTEYRMMAKL----QNLNDYDLTHLHSAVSAGEPLNREV 310
Cdd:cd05967  296 LH----GATTVLYEGKPVgtpdPGAFWRVIEKYQVNALFTAPTAIRAIRKEdpdgKYIKKYDLSSLRTLFLAGERLDPPT 371
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 311 VEQFKKYFNLTVRDGYGQTES-----TLLIGfLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDL-PAL 384
Cdd:cd05967  372 LEWAENTLGVPVIDHWWQTETgwpitANPVG-LEPLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLPLpPGC 450
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 385 FKGYYKEPERTAKAQTGK---YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHD 461
Cdd:cd05967  451 LLTLWKNDERFKKLYLSKfpgYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDE 530
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 612673656 462 IRGNIVKAFVILQDH-TAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD-AEIEKW 531
Cdd:cd05967  531 LKGQVPLGLVVLKEGvKITAEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKiADGEDY 602
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
32-525 2.13e-75

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 247.51  E-value: 2.13e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRS----IATYelyiAALKLGVAIIPCSEML 107
Cdd:PRK07656  19 GDKEAYVF-----GDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSphwvIAAL----GALKAGAVVVPLNTRY 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 108 RTKDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASNKLEMADTTR-------DDMA 180
Cdd:PRK07656  90 TADEAAYILARGDAKALFVLGLFLGVDYSATTRLPALEHVVICETEEDDPHTEKMKTFTDFLAAGDPAErapevdpDDVA 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 181 ILSYTSGTTGNPKAVThchgwgFAHLQM--APKHW---LCINEDDLVWATaapgwqkwvwSPFLSVLG----------SG 245
Cdd:PRK07656 170 DILFTSGTTGRPKGAM------LTHRQLlsNAADWaeyLGLTEGDRYLAA----------NPFFHVFGykagvnaplmRG 233
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 246 ATAFVFNgRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNL-TVRD 324
Cdd:PRK07656 234 ATILPLP-VFDPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAEDLSSLRLAVTGAASMPVALLERFESELGVdIVLT 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 325 GYGQTESTLLIGF--LKDTPQRI-GSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTG 401
Cdd:PRK07656 313 GYGLSEASGVTTFnrLDDDRKTVaGTIGTAIAGVENKIVNELGEEVPVGEVGELLV--RGPNVMKGYYDDPEATAAAIDA 390
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 402 KYYI-TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGD 480
Cdd:PRK07656 391 DGWLhTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDERLGEVGKAYVVLKPGAELT 470
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*
gi 612673656 481 DtlvKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK07656 471 E---EELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
48-520 8.73e-74

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 240.84  E-value: 8.73e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIAL 127
Cdd:cd05935    1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVG 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 128 epftvefekikeydaltkfviagqkdgwisleaekenasnklemadTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLq 207
Cdd:cd05935   81 ----------------------------------------------SELDDLALIPYTSGTTGLPKGCMHTHFSAAANA- 113
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 208 MAPKHWLCINEDDLVWATAapgwqkwvwsPFLSVLG----------SGATaFVFNGRFSPETYLELLQKYQINVLCCTPT 277
Cdd:cd05935  114 LQSAVWTGLTPSDVILACL----------PLFHVTGfvgslntavyVGGT-YVLMARWDRETALELIEKYKVTFWTNIPT 182
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 278 EYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFlkDTPQRIGSMGKGIPGSSV 357
Cdd:cd05935  183 MLVDLLATPEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGLTETMSQTHT--NPPLRPKLQCLGIP*FGV 260
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 358 T--VVDDE-GNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQT----GKYYITGDLAHVDDDGYFWFEGRRDDIIIS 430
Cdd:cd05935  261 DarVIDIEtGRELPPNEVGEIVV--RGPQIFKGYWNRPEETEESFIeikgRRFFRTGDLGYMDEEGYFFFVDRVKRMINV 338
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 431 SGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTlVKELQQFVKNEVAPYKYPREIEFVDDL 510
Cdd:cd05935  339 SGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYRGKVT-EEDIIEWAREQMAAYKYPREVEFVDEL 417
                        490
                 ....*....|
gi 612673656 511 PKTNSGKIRR 520
Cdd:cd05935  418 PRSASGKILW 427
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
44-533 1.13e-72

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 239.81  E-value: 1.13e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  44 GEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINA 123
Cdd:PRK08276   7 PSGEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKV 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 124 VI---ALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASNKLEMADTTRDDMAilsYTSGTTGNPKAV----T 196
Cdd:PRK08276  87 LIvsaALADTAAELAAELPAGVPLLLVVAGPVPGFRSYEEALAAQPDTPIADETAGADML---YSSGTTGRPKGIkrplP 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 197 HCHGWGFAHLQMA-PKHWLCINEDDLVWATA-----APGwqkwVWSpfLSVLGSGATAfVFNGRFSPETYLELLQKYQIN 270
Cdd:PRK08276 164 GLDPDEAPGMMLAlLGFGMYGGPDSVYLSPAplyhtAPL----RFG--MSALALGGTV-VVMEKFDAEEALALIERYRVT 236
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 271 VLCCTPTEYRMMAKL--QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEStllIGFLKDTPQ----R 344
Cdd:PRK08276 237 HSQLVPTMFVRMLKLpeEVRARYDVSSLRVAIHAAAPCPVEVKRAMIDWWGPIIHEYYASSEG---GGVTVITSEdwlaH 313
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 345 IGSMGKGIPGSsVTVVDDEGNSVPANTKGNIAVPLDLPAlFKgYYKEPERTAKAQTGKYYIT-GDLAHVDDDGYFWFEGR 423
Cdd:PRK08276 314 PGSVGKAVLGE-VRILDEDGNELPPGEIGTVYFEMDGYP-FE-YHNDPEKTAAARNPHGWVTvGDVGYLDEDGYLYLTDR 390
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 424 RDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPRE 503
Cdd:PRK08276 391 KSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRS 470
                        490       500       510
                 ....*....|....*....|....*....|
gi 612673656 504 IEFVDDLPKTNSGKIRRVELRDAEIEKWQQ 533
Cdd:PRK08276 471 IDFEDELPRTPTGKLYKRRLRDRYWEGRQR 500
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
32-523 4.50e-72

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 236.66  E-value: 4.50e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcsemLrtkD 111
Cdd:cd05930    1 PDAVAVVD-----GDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVP----L---D 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQY---RITHgeinaVIAlepftvefekikeyDALTKFVIagqkdgwisleaekenasnklemadTTRDDMAILSYTSGT 188
Cdd:cd05930   69 PSYpaeRLAY-----ILE--------------DSGAKLVL-------------------------TDPDDLAYVIYTSGS 104
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 189 TGNPKAV--THChgwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSvLGSGATAFVF--NGRFSPETYLELL 264
Cdd:cd05930  105 TGKPKGVmvEHR---GLVNLLLWMQEAYPLTPGDRVLQFTSFSFDVSVWEIFGA-LLAGATLVVLpeEVRKDPEALADLL 180
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 265 QKYQINVLCCTPTEYRMMakLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTESTLLIGFLKDTPQ 343
Cdd:cd05930  181 AEEGITVLHLTPSLLRLL--LQELELAALPSLRLVLVGGEALPPDLVRRWRELLpGARLVNLYGPTEATVDATYYRVPPD 258
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 344 RIGSM----GKGIPGSSVTVVDDEGNSVPANTKGNIAV--PldlpALFKGYYKEPERTAK-------AQTGKYYITGDLA 410
Cdd:cd05930  259 DEEDGrvpiGRPIPNTRVYVLDENLRPVPPGVPGELYIggA----GLARGYLNRPELTAErfvpnpfGPGERMYRTGDLV 334
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 411 HVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVKELQQF 490
Cdd:cd05930  335 RWLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGDGEKRLVAYVVPDE---GGELDEEELRAH 411
                        490       500       510
                 ....*....|....*....|....*....|...
gi 612673656 491 VKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd05930  412 LAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
32-525 9.01e-69

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 229.51  E-value: 9.01e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILyhnTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd05926    1 PDAPALV---VPGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAE 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIAlePFTVEFEKIKeydALTKFVIAGQKDGWIS------LEAEKENASNKLEMADTTR-----DDMA 180
Cdd:cd05926   78 FEFYLADLGSKLVLT--PKGELGPASR---AASKLGLAILELALDVgvliraPSAESLSNLLADKKNAKSEgvplpDDLA 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 181 ILSYTSGTTGNPKAV--THchgwgfAHLQMAPKHW---LCINEDDLVWATAapgwqkwvwsPF----------LSVLGSG 245
Cdd:cd05926  153 LILHTSGTTGRPKGVplTH------RNLAASATNItntYKLTPDDRTLVVM----------PLfhvhglvaslLSTLAAG 216
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 246 ATAfVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYD-LTHLHSAVSAGEPLNREVVEQFKKYFNLTVRD 324
Cdd:cd05926  217 GSV-VLPPRFSASTFWPDVRDYNATWYTAVPTIHQILLNRPEPNPESpPPKLRFIRSCSASLPPAVLEALEATFGAPVLE 295
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 325 GYGQTESTLLIGF--LKDTPQRIGSMGKGIpGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAK-AQTG 401
Cdd:cd05926  296 AYGMTEAAHQMTSnpLPPGPRKPGSVGKPV-GVEVRILDEDGEILPPGVVGEIC--LRGPNVTRGYLNNPEANAEaAFKD 372
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 402 KYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDD 481
Cdd:cd05926  373 GWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASVTE 452
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 612673656 482 tlvKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd05926  453 ---EELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
48-523 2.19e-66

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 223.27  E-value: 2.19e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRIThgEINAVIAl 127
Cdd:cd05904   32 ALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVK--DSGAKLA- 108
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 128 epFTV--EFEKIKEYDALTkfVIAGQ--KDGWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGF 203
Cdd:cd05904  109 --FTTaeLAEKLASLALPV--VLLDSaeFDSLSFSDLLFEADEAEPPVVVIKQDDVAALLYSSGTTGRSKGVMLTHRNLI 184
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 204 AHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPF-LSVLGSGATAFVFnGRFSPETYLELLQKYQINVLCCTPTEYRMM 282
Cdd:cd05904  185 AMVAQFVAGEGSNSDSEDVFLCVLPMFHIYGLSSFaLGLLRLGATVVVM-PRFDLEELLAAIERYKVTHLPVVPPIVLAL 263
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 283 AKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTEST--LLIGFLKD-TPQRIGSMGKGIPGSSVT 358
Cdd:cd05904  264 VKSPIVDKYDLSSLRQIMSGAAPLGKELIEAFRAKFpNVDLGQGYGMTESTgvVAMCFAPEkDRAKYGSVGRLVPNVEAK 343
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 359 VVD-DEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYI-TGDLAHVDDDGYFWFEGRRDDIIISSGYTIG 436
Cdd:cd05904  344 IVDpETGESLPPNQTGELWI--RGPSIMKGYLNNPEATAATIDKEGWLhTGDLCYIDEDGYLFIVDRLKELIKYKGFQVA 421
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 437 PFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSG 516
Cdd:cd05904  422 PAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRK---PGSSLTEDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPSG 498

                 ....*..
gi 612673656 517 KIRRVEL 523
Cdd:cd05904  499 KILRKEL 505
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
9-526 3.02e-66

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 225.83  E-value: 3.02e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   9 QKSALFAPEKYNIISEIEKYS--HIPDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIAT 86
Cdd:cd05968   50 PWAAWFVGGRMNIVEQLLDKWlaDTRTRPALRWEGEDGTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEI 129
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  87 YELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFT---------VEFEKIKEYDALTKFVI----AGQKD 153
Cdd:cd05968  130 VPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAEAKALITADGFTrrgrevnlkEEADKACAQCPTVEKVVvvrhLGNDF 209
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 154 GW-----ISLEAEKENASNKLEmaDTTRDDMAILSYTSGTTGNPKAVTHCHGwGFAhLQMAPKHWLCIN---EDDLVWAT 225
Cdd:cd05968  210 TPakgrdLSYDEEKETAGDGAE--RTESEDPLMIIYTSGTTGKPKGTVHVHA-GFP-LKAAQDMYFQFDlkpGDLLTWFT 285
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 226 AApgwqKWVWSPFLsVLGS---GATAFVFNG---RFSPETYLELLQKYQINVLCCTPTEYR--MMAKLQNLNDYDLTHLH 297
Cdd:cd05968  286 DL----GWMMGPWL-IFGGlilGATMVLYDGapdHPKADRLWRMVEDHEITHLGLSPTLIRalKPRGDAPVNAHDLSSLR 360
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 298 SAVSAGEPLNREVVeqfkKYFNLTVRDGY-------GQTE-STLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPa 369
Cdd:cd05968  361 VLGSTGEPWNPEPW----NWLFETVGKGRnpiinysGGTEiSGGILGNVLIKPIKPSSFNGPVPGMKADVLDESGKPAR- 435
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 370 NTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKY---YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTN 446
Cdd:cd05968  436 PEVGELVLLAPWPGMTRGFWRDEDRYLETYWSRFdnvWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNA 515
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 447 HPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDA 526
Cdd:cd05968  516 HPAVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTEALAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIRAA 595
PRK08316 PRK08316
acyl-CoA synthetase; Validated
30-525 3.04e-66

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 223.66  E-value: 3.04e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  30 HIPDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT 109
Cdd:PRK08316  23 RYPDKTALVF-----GDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTG 97
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 110 KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQ------KDGWISLEAEKENASNKLEMADTTRDDMAILS 183
Cdd:PRK08316  98 EELAYILDHSGARAFLVDPALAPTAEAALALLPVDTLILSLVlggreaPGGWLDFADWAEAGSVAEPDVELADDDLAQIL 177
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 184 YTSGTTGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWAtAAPGW---QKWVW-SPFLSVlgsGATAFVFNGRfSPET 259
Cdd:PRK08316 178 YTSGTESLPKGAMLTHR-ALIAEYVSCIVAGDMSADDIPLH-ALPLYhcaQLDVFlGPYLYV---GATNVILDAP-DPEL 251
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 260 YLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTEstllIGFL 338
Cdd:PRK08316 252 ILRTIEAERITSFFAPPTVWISLLRHPDFDTRDLSSLRKGYYGASIMPVEVLKELRERLpGLRFYNCYGQTE----IAPL 327
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 339 ------KDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHV 412
Cdd:PRK08316 328 atvlgpEEHLRRPGSAGRPVLNVETRVVDDDGNDVAPGEVGEIV--HRSPQLMLGYWDDPEKTAEAFRGGWFHSGDLGVM 405
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 413 DDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTLVKELQQFVK 492
Cdd:PRK08316 406 DEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPK---AGATVTEDELIAHCR 482
                        490       500       510
                 ....*....|....*....|....*....|...
gi 612673656 493 NEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK08316 483 ARLAGFKVPKRVIFVDELPRNPSGKILKRELRE 515
PRK06188 PRK06188
acyl-CoA synthetase; Validated
21-524 3.40e-65

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 220.63  E-value: 3.40e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  21 IISEIEKYshiPDKKAILyhnteGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAI 100
Cdd:PRK06188  18 LVSALKRY---PDRPALV-----LGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAAQLAGLRR 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 101 IPCSEMLRTKDLQYRITHGEINA-VIALEPFTVEFEKIKE-YDALTKFVIAGQKDGWISLEAEKENASNKLEMADTTRDD 178
Cdd:PRK06188  90 TALHPLGSLDDHAYVLEDAGISTlIVDPAPFVERALALLArVPSLKHVLTLGPVPDGVDLLAAAAKFGPAPLVAAALPPD 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 179 MAILSYTSGTTGNPKAVTHCH--GWGFAHLQMAPKHWlcinEDDLVWATAAP-----GwqkwvwSPFLSVLGSGATAFVF 251
Cdd:PRK06188 170 IAGLAYTGGTTGKPKGVMGTHrsIATMAQIQLAEWEW----PADPRFLMCTPlshagG------AFFLPTLLRGGTVIVL 239
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 252 NGrFSPETYLELLQKYQINVLCCTPTE-YRMMaKLQNLNDYDLTHLHSAVSAGEPLN----REVVEQFKKYFNLTvrdgY 326
Cdd:PRK06188 240 AK-FDPAEVLRAIEEQRITATFLVPTMiYALL-DHPDLRTRDLSSLETVYYGASPMSpvrlAEAIERFGPIFAQY----Y 313
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 327 GQTESTLLIGFLK------DTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAV--PLdlpaLFKGYYKEPERTAKA 398
Cdd:PRK06188 314 GQTEAPMVITYLRkrdhdpDDPKRLTSCGRPTPGLRVALLDEDGREVAQGEVGEICVrgPL----VMDGYWNRPEETAEA 389
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 399 QTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtA 478
Cdd:PRK06188 390 FRDGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLR---P 466
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*.
gi 612673656 479 GDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK06188 467 GAAVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKALR 512
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
32-525 7.37e-65

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 218.71  E-value: 7.37e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHntegeDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd12118   18 PDRTSIVYG-----DRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEE 92
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFtvefekikEYDALtkfvIAGQKDGWISLEAEKENasnklemadttrdDMAILSYTSGTTGN 191
Cdd:cd12118   93 IAFILRHSEAKVLFVDREF--------EYEDL----LAEGDPDFEWIPPADEW-------------DPIALNYTSGTTGR 147
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGWGFAHLQMAPKHWLCinEDDLVWATAAP-----GWQkWVWSPFlsvlGSGATAfVFNGRFSPETYLELLQK 266
Cdd:cd12118  148 PKGVVYHHRGAYLNALANILEWEM--KQHPVYLWTLPmfhcnGWC-FPWTVA----AVGGTN-VCLRKVDAKAIYDLIEK 219
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 267 YQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKyFNLTVRDGYGQTEST--LLIGFLKD---- 340
Cdd:cd12118  220 HKVTHFCGAPTVLNMLANAPPSDARPLPHRVHVMTAGAPPPAAVLAKMEE-LGFDVTHVYGLTETYgpATVCAWKPewde 298
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 341 --TPQRIGSM---GKGIPGSS-VTVVDDEGN-SVPANTK--------GNIavpldlpaLFKGYYKEPERTAKAQTGKYYI 405
Cdd:cd12118  299 lpTEERARLKarqGVRYVGLEeVDVLDPETMkPVPRDGKtigeivfrGNI--------VMKGYLKNPEATAEAFRGGWFH 370
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 406 TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVK 485
Cdd:cd12118  371 SGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKE---GAKVTEE 447
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 612673656 486 ELQQFVKNEVAPYKYPREIEFvDDLPKTNSGKIRRVELRD 525
Cdd:cd12118  448 EIIAFCREHLAGFMVPKTVVF-GELPKTSTGKIQKFVLRD 486
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
48-525 1.48e-63

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 214.08  E-value: 1.48e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  48 SVTYQQLIEQSNKVGNVL-ASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIa 126
Cdd:cd05941   11 SITYADLVARAARLANRLlALGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVITDSEPSLVL- 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 127 lepftvefekikeydaltkfviagqkdgwisleaekenasnklemadttrdDMAILSYTSGTTGNPKAVTHCHGWGFAHL 206
Cdd:cd05941   90 ---------------------------------------------------DPALILYTSGTTGRPKGVVLTHANLAANV 118
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 207 QMAPKHWLCINED------------DLVWATAAPGWqkwvwspflsvlgSGATaFVFNGRFSPETYLELLQKYQINVLCC 274
Cdd:cd05941  119 RALVDAWRWTEDDvllhvlplhhvhGLVNALLCPLF-------------AGAS-VEFLPKFDPKEVAISRLMPSITVFMG 184
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 275 TPTEYRMMAKLQNLNDYDLTHLHSA--------VSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIG 346
Cdd:cd05941  185 VPTIYTRLLQYYEAHFTDPQFARAAaaerlrlmVSGSAALPVPTLEEWEAITGHTLLERYGMTEIGMALSNPLDGERRPG 264
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 347 SMGKGIPGSSVTVVDDEGNS-VPANTKGNIAVplDLPALFKGYYKEPERTAKAQTG-KYYITGDLAHVDDDGYFWFEGR- 423
Cdd:cd05941  265 TVGMPLPGVQARIVDEETGEpLPRGEVGEIQV--RGPSVFKEYWNKPEATKEEFTDdGWFKTGDLGVVDEDGYYWILGRs 342
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 424 RDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLvkELQQFVKNEVAPYKYPRE 503
Cdd:cd05941  343 SVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGAAALSLE--ELKEWAKQRLAPYKRPRR 420
                        490       500
                 ....*....|....*....|..
gi 612673656 504 IEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd05941  421 LILVDELPRNAMGKVNKKELRK 442
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
48-524 1.53e-63

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 216.95  E-value: 1.53e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVI-- 125
Cdd:PRK07786  42 TTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLVSDCGAHVVVte 121
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 126 -ALEPFTVEFEKIKEydALTKFVIAG--QKDGWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKavthchGWG 202
Cdd:PRK07786 122 aALAPVATAVRDIVP--LLSTVVVAGgsSDDSVLGYEDLLAEAGPAHAPVDIPNDSPALIMYTSGTTGRPK------GAV 193
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 203 FAHLQMAPKHWLCI------NEDDLvwataapgwqKWVWSPFLSV--LGSGATAFVFN--------GRFSPETYLELLQK 266
Cdd:PRK07786 194 LTHANLTGQAMTCLrtngadINSDV----------GFVGVPLFHIagIGSMLPGLLLGaptviyplGAFDPGQLLDVLEA 263
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 267 YQINVLCCTPTEYRMMAKLQNLNDYDLThLH----SAVSAGEPLNREVVEQFKKYFNLTVrdgYGQTE----STLLIGfl 338
Cdd:PRK07786 264 EKVTGIFLVPAQWQAVCAEQQARPRDLA-LRvlswGAAPASDTLLRQMAATFPEAQILAA---FGQTEmspvTCMLLG-- 337
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 339 KDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYF 418
Cdd:PRK07786 338 EDAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIV--YRAPTLMSGYWNNPEATAEAFAGGWFHSGDLVRQDEEGYV 415
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 419 WFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtAGDDTLVKELQQFVKNEVAPY 498
Cdd:PRK07786 416 WVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRN--DDAALTLEDLAEFLTDRLARY 493
                        490       500
                 ....*....|....*....|....*.
gi 612673656 499 KYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK07786 494 KHPKALEIVDALPRNPAGKVLKTELR 519
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
32-525 5.27e-63

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 214.55  E-value: 5.27e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTeGEdiSVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK13391  11 PDKPAVIMAST-GE--VVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAE 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVI----ALEpftVEFEKIKEYDALTKFVIA---GQKDGWISLEAEKENASnklemaDTTRDDMAI--- 181
Cdd:PRK13391  88 AAYIVDDSGARALItsaaKLD---VARALLKQCPGVRHRLVLdgdGELEGFVGYAEAVAGLP------ATPIADESLgtd 158
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 182 LSYTSGTTGNPKAVthchgwgFAHL-QMAPKHWLCINE---------DDLVWATAAP----GWQKWVwspfLSVLGSGAT 247
Cdd:PRK13391 159 MLYSSGTTGRPKGI-------KRPLpEQPPDTPLPLTAflqrlwgfrSDMVYLSPAPlyhsAPQRAV----MLVIRLGGT 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 248 AFVFNgRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQN--LNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDG 325
Cdd:PRK13391 228 VIVME-HFDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEevRDKYDLSSLEVAIHAAAPCPPQVKEQMIDWWGPIIHEY 306
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 326 YGQTEStllIGFLK-DTPQ---RIGSMGKGIPGSsVTVVDDEGNSVPANTKGNIavpldlpaLFKG-----YYKEPERTA 396
Cdd:PRK13391 307 YAATEG---LGFTAcDSEEwlaHPGTVGRAMFGD-LHILDDDGAELPPGEPGTI--------WFEGgrpfeYLNDPAKTA 374
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 397 KAQT--GKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQ 474
Cdd:PRK13391 375 EARHpdGTWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPV 454
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|.
gi 612673656 475 DHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK13391 455 DGVDPGPALAAELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLRD 505
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
48-525 1.09e-62

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 211.05  E-value: 1.09e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcsemlrtkdLQYRITHGEINavial 127
Cdd:cd05912    1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVL---------LNTRLTPNELA----- 66
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 128 epftvefekikeydaltkfviagqkdgwisleaekenasNKLEMADTTRDDMAILSYTSGTTGNPKAVthchgwgfahLQ 207
Cdd:cd05912   67 ---------------------------------------FQLKDSDVKLDDIATIMYTSGTTGKPKGV----------QQ 97
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 208 MAPKHW---------LCINEDDlVWATAAPGWQKWVWSPFLSVLGSGATAFVFNgRFSPETYLELLQKYQINVLCCTPTe 278
Cdd:cd05912   98 TFGNHWwsaigsalnLGLTEDD-NWLCALPLFHISGLSILMRSVIYGMTVYLVD-KFDAEQVLHLINSGKVTIISVVPT- 174
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 279 yrMMAKL-QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYfNLTVRDGYGQTESTLLIGFLK--DTPQRIGSMGKGIPGS 355
Cdd:cd05912  175 --MLQRLlEILGEGYPNNLRCILLGGGPAPKPLLEQCKEK-GIPVYQSYGMTETCSQIVTLSpeDALNKIGSAGKPLFPV 251
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 356 SVTVVDDEGnsvPANTKGNIAvpLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTI 435
Cdd:cd05912  252 ELKIEDDGQ---PPYEVGEIL--LKGPNVTKGYLNRPDATEESFENGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENI 326
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 436 GPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtagDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS 515
Cdd:cd05912  327 YPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSE-----RPISEEELIAYCSEKLAKYKVPKKIYFVDELPRTAS 401
                        490
                 ....*....|
gi 612673656 516 GKIRRVELRD 525
Cdd:cd05912  402 GKLLRHELKQ 411
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
48-524 5.50e-62

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 209.93  E-value: 5.50e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIAL 127
Cdd:cd05903    1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVP 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 128 EPFtvefekikeydaltkfviaGQKDgwisleaekenasnKLEMADttrdDMAILSYTSGTTGNPKAVTHCHGWGFAHLQ 207
Cdd:cd05903   81 ERF-------------------RQFD--------------PAAMPD----AVALLLFTSGTTGEPKGVMHSHNTLSASIR 123
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 208 MAPKHWLcINEDDLVWaTAAP-----GWQKWVWSPFLSvlgsGATAfVFNGRFSPETYLELLQKYQINVLCCTPTEYRMM 282
Cdd:cd05903  124 QYAERLG-LGPGDVFL-VASPmahqtGFVYGFTLPLLL----GAPV-VLQDIWDPDKALALMREHGVTFMMGATPFLTDL 196
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 283 AKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTP--QRIGSMGKGIPGSSVTVV 360
Cdd:cd05903  197 LNAVEEAGEPLSRLRTFVCGGATVPRSLARRAAELLGAKVCSAYGSTECPGAVTSITPAPedRRLYTDGRPLPGVEIKVV 276
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 361 DDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEV 440
Cdd:cd05903  277 DDTGATLAPGVEGELLS--RGPSVFLGYLDRPDLTADAAPEGWFRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEV 354
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 441 EDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQD-HTAGDDTLVKELQqfvKNEVAPYKYPREIEFVDDLPKTNSGKIR 519
Cdd:cd05903  355 EDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSgALLTFDELVAYLD---RQGVAKQYWPERLVHVDDLPRTPSGKVQ 431

                 ....*
gi 612673656 520 RVELR 524
Cdd:cd05903  432 KFRLR 436
PRK06178 PRK06178
acyl-CoA synthetase; Validated
32-523 2.07e-61

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 211.82  E-value: 2.07e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEgedisVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK06178  47 PQRPAIIFYGHV-----ITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHE 121
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKD----------------------GWISLEAEKENASNKL 169
Cdd:PRK06178 122 LSYELNDAGAEVLLALDQLAPVVEQVRAETSLRHVIVTSLADvlpaeptlplpdslraprlaaaGAIDLLPALRACTAPV 201
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 170 EMADTTRDDMAILSYTSGTTGNPKAVTHCHGwgfahlqmapkhwlcinedDLVWATAA------PGWQKWVWSPFLSV-- 241
Cdd:PRK06178 202 PLPPPALDALAALNYTGGTTGMPKGCEHTQR-------------------DMVYTAAAayavavVGGEDSVFLSFLPEfw 262
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 242 -----------LGSGATaFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHS--AVSAGEPLNR 308
Cdd:PRK06178 263 iagenfgllfpLFSGAT-LVLLARWDAVAFMAAVERYRVTRTVMLVDNAVELMDHPRFAEYDLSSLRQvrVVSFVKKLNP 341
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 309 EVVEQFKKYFNLTVRDG-YGQTES----TLLIGFLKDTPQRIGS---MGKGIPGSSVTVVD-DEGNSVPANTKGNIAVpl 379
Cdd:PRK06178 342 DYRQRWRALTGSVLAEAaWGMTEThtcdTFTAGFQDDDFDLLSQpvfVGLPVPGTEFKICDfETGELLPLGAEGEIVV-- 419
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 380 DLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKP 459
Cdd:PRK06178 420 RTPSLLKGYWNKPEATAEALRDGWLHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRP 499
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 612673656 460 HDIRGNIVKAFVILQDhtaGDDTLVKELQQFVKNEVAPYKYPrEIEFVDDLPKTNSGKIRRVEL 523
Cdd:PRK06178 500 DPDKGQVPVAFVQLKP---GADLTAAALQAWCRENMAVYKVP-EIRIVDALPMTATGKVRKQDL 559
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
46-525 3.44e-61

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 211.03  E-value: 3.44e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  46 DISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPrSIATYELYIAA-LKLGVAIIPCSEMLRTKDLQYRITHGEINAV 124
Cdd:PRK07059  46 GKAITYGELDELSRALAAWLQSRGLAKGARVAIMMP-NVLQYPVAIAAvLRAGYVVVNVNPLYTPRELEHQLKDSGAEAI 124
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 125 IALEPFTVEFEKIKEYDALTKFVIAGQKD-----GWI---------------SL-------EAEKENASNKLEMADTTRD 177
Cdd:PRK07059 125 VVLENFATTVQQVLAKTAVKHVVVASMGDllgfkGHIvnfvvrrvkkmvpawSLpghvrfnDALAEGARQTFKPVKLGPD 204
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 178 DMAILSYTSGTTGNPKAVTHCHGWGFA-----HLQMAPKHWLCINEDDLVWATAAPGWQKWVWS--PFLSVLGSGATAFV 250
Cdd:PRK07059 205 DVAFLQYTGGTTGVSKGATLLHRNIVAnvlqmEAWLQPAFEKKPRPDQLNFVCALPLYHIFALTvcGLLGMRTGGRNILI 284
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 251 FNGRFSPeTYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTE 330
Cdd:PRK07059 285 PNPRDIP-GFIKELKKYQVHIFPAVNTLYNALLNNPDFDKLDFSKLIVANGGGMAVQRPVAERWLEMTGCPITEGYGLSE 363
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 -STLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGK-YYITGD 408
Cdd:PRK07059 364 tSPVATCNPVDATEFSGTIGLPLPSTEVSIRDDDGNDLPLGEPGEICI--RGPQVMAGYWNRPDETAKVMTADgFFRTGD 441
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 409 LAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDtlvkELQ 488
Cdd:PRK07059 442 VGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFVVKKDPALTEE----DVK 517
                        490       500       510
                 ....*....|....*....|....*....|....*..
gi 612673656 489 QFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK07059 518 AFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELRD 554
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
32-530 1.21e-60

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 207.51  E-value: 1.21e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK03640  16 PDRTAIEF-----EEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREE 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFTVEFEkikeydaltkfviAGQKDGWISLEAEKENASNKLEMADTtrDDMAILSYTSGTTGN 191
Cdd:PRK03640  91 LLWQLDDAEVKCLITDDDFEAKLI-------------PGISVKFAELMNGPKEEAEIQEEFDL--DEVATIMYTSGTTGK 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVthchgwgfahLQMAPKHW---------LCINEDDlVWATAAP-----GwqkwvwspfLSVLGSGatafVFNG---- 253
Cdd:PRK03640 156 PKGV----------IQTYGNHWwsavgsalnLGLTEDD-CWLAAVPifhisG---------LSILMRS----VIYGmrvv 211
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 254 ---RFSPETYLELLQKYQINVLCCTPTeyrMMAKLqnLNDYDLTHLHSAVSA----GEPLNREVVEQFKKYfNLTVRDGY 326
Cdd:PRK03640 212 lveKFDAEKINKLLQTGGVTIISVVST---MLQRL--LERLGEGTYPSSFRCmllgGGPAPKPLLEQCKEK-GIPVYQSY 285
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 327 GQTESTLLIGFL--KDTPQRIGSMGKGIPGSSVTVVDDeGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYY 404
Cdd:PRK03640 286 GMTETASQIVTLspEDALTKLGSAGKPLFPCELKIEKD-GVVVPPFEEGEIVV--KGPNVTKGYLNREDATRETFQDGWF 362
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 405 ITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILqDHTAGDDtlv 484
Cdd:PRK03640 363 KTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFVVK-SGEVTEE--- 438
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*.
gi 612673656 485 kELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEK 530
Cdd:PRK03640 439 -ELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQLVEEM 483
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
32-524 3.85e-60

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 206.78  E-value: 3.85e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTeGEDISvtYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK13390  11 PDRPAVIVAET-GEQVS--YRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPE 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFTVEFEKIKEyDALTKFVIAGQKDGWISLEAEKENASNKLemadTTRDDMAILSYTSGTTGN 191
Cdd:PRK13390  88 ADYIVGDSGARVLVASAALDGLAAKVGA-DLPLRLSFGGEIDGFGSFEAALAGAGPRL----TEQPCGAVMLYSSGTTGF 162
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVT------HCHGWGFAHLQMApKHWLCINEDDlVWATAAPGWQK--WVWSPFLSVLGSgatAFVFNGRFSPETYLEL 263
Cdd:PRK13390 163 PKGIQpdlpgrDVDAPGDPIVAIA-RAFYDISESD-IYYSSAPIYHAapLRWCSMVHALGG---TVVLAKRFDAQATLGH 237
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 264 LQKYQINVLCCTPTeyrMMAKLQNLND-----YDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIgfL 338
Cdd:PRK13390 238 VERYRITVTQMVPT---MFVRLLKLDAdvrtrYDVSSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEYYSSTEAHGMT--F 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 339 KDTPQRI---GSMGKGIPGSsVTVVDDEGNSVPANTKGNIAVPLDlpALFKGYYKEPERTAKAQ--TGKYYIT-GDLAHV 412
Cdd:PRK13390 313 IDSPDWLahpGSVGRSVLGD-LHICDDDGNELPAGRIGTVYFERD--RLPFRYLNDPEKTAAAQhpAHPFWTTvGDLGSV 389
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 413 DDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVK 492
Cdd:PRK13390 390 DEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTR 469
                        490       500       510
                 ....*....|....*....|....*....|..
gi 612673656 493 NEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK13390 470 SRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
26-524 6.21e-60

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 206.46  E-value: 6.21e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  26 EKYShipDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSE 105
Cdd:PRK08008  18 DVYG---HKTALIFESSGGVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINA 94
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 106 MLRTKDLQYRITHGEINAVIALEPFTVEFEKIKEYDA--LTKFVIAG----QKDGWISLEAEKENASNKLEMADT-TRDD 178
Cdd:PRK08008  95 RLLREESAWILQNSQASLLVTSAQFYPMYRQIQQEDAtpLRHICLTRvalpADDGVSSFTQLKAQQPATLCYAPPlSTDD 174
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 179 MAILSYTSGTTGNPKAV--THCHgWGFAHLQMApkhWLCINEDDLVWATAAPGW----QKWVWSPFLSVlgsGATaFVFN 252
Cdd:PRK08008 175 TAEILFTSGTTSRPKGVviTHYN-LRFAGYYSA---WQCALRDDDVYLTVMPAFhidcQCTAAMAAFSA---GAT-FVLL 246
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 253 GRFSPETYLELLQKYQINVLCCTPteyrMMAK---LQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQT 329
Cdd:PRK08008 247 EKYSARAFWGQVCKYRATITECIP----MMIRtlmVQPPSANDRQHCLREVMFYLNLSDQEKDAFEERFGVRLLTSYGMT 322
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 330 EStlLIGFLKDTP---QRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPlDLPA--LFKGYYKEPERTAKAQTGK-Y 403
Cdd:PRK08008 323 ET--IVGIIGDRPgdkRRWPSIGRPGFCYEAEIRDDHNRPLPAGEIGEICIK-GVPGktIFKEYYLDPKATAKVLEADgW 399
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILqdhTAGDDTL 483
Cdd:PRK08008 400 LHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVL---NEGETLS 476
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 612673656 484 VKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK08008 477 EEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
29-539 6.98e-60

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 207.58  E-value: 6.98e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  29 SHIPDKKAILYHnteGEDIsvTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLR 108
Cdd:PRK06710  35 SRYPEKKALHFL---GKDI--TFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYT 109
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 TKDLQYRITHGEINAVIALE---------------------------PF----TVEFEKIKEYDALTKFVIAGQKDGWIS 157
Cdd:PRK06710 110 ERELEYQLHDSGAKVILCLDlvfprvtnvqsatkiehvivtriadflPFpknlLYPFVQKKQSNLVVKVSESETIHLWNS 189
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 158 LEAEKENAsnkLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMApKHWL--CINEDDLVWATAapgwqkwvw 235
Cdd:PRK06710 190 VEKEVNTG---VEVPCDPENDLALLQYTGGTTGFPKGVMLTHKNLVSNTLMG-VQWLynCKEGEEVVLGVL--------- 256
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 236 sPFLSVLGSGAT---------AFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPL 306
Cdd:PRK06710 257 -PFFHVYGMTAVmnlsimqgyKMVLIPKFDMKMVFEAIKKHKVTLFPGAPTIYIALLNSPLLKEYDISSIRACISGSAPL 335
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 307 NREVVEQFKKYFNLTVRDGYGQTESTLLI--GFL--KDTPqriGSMGKGIPGSSVTVVDDE-GNSVPANTKGNIAVplDL 381
Cdd:PRK06710 336 PVEVQEKFETVTGGKLVEGYGLTESSPVThsNFLweKRVP---GSIGVPWPDTEAMIMSLEtGEALPPGEIGEIVV--KG 410
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 382 PALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHD 461
Cdd:PRK06710 411 PQIMKGYWNKPEETAAVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDP 490
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 612673656 462 IRGNIVKAFVILQDHTAGDDtlvKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIrrveLRDAEIEKWQQQKDSNQ 539
Cdd:PRK06710 491 YRGETVKAFVVLKEGTECSE---EELNQFARKYLAAYKVPKVYEFRDELPKTTVGKI----LRRVLIEEEKRKNEDEQ 561
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
25-525 1.88e-59

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 205.84  E-value: 1.88e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  25 IEKYSHIPDKKAILYHNTEgedISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCS 104
Cdd:cd17642   24 MKRYASVPGTIAFTDAHTG---VNYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTN 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 105 EMLRTKDLQYRI----------THGEINAVIALEPFTVEFEKI---------KEYDALTKFvIAGQKDGWISLEAEKENA 165
Cdd:cd17642  101 DIYNERELDHSLniskptivfcSKKGLQKVLNVQKKLKIIKTIiildskedyKGYQCLYTF-ITQNLPPGFNEYDFKPPS 179
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 166 SNKlemadttRDDMAILSYTSGTTGNPKAVTHCHG---WGFAHlQMAPKHWLCINEDDLVwATAAPGWQKWVWSPFLSVL 242
Cdd:cd17642  180 FDR-------DEQVALIMNSSGSTGLPKGVQLTHKnivARFSH-ARDPIFGNQIIPDTAI-LTVIPFHHGFGMFTTLGYL 250
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 243 GSGATaFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLT- 321
Cdd:cd17642  251 ICGFR-VVLMYKFEEELFLRSLQDYKVQSALLVPTLFAFFAKSTLVDKYDLSNLHEIASGGAPLSKEVGEAVAKRFKLPg 329
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 322 VRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVD-DEGNSVPANTKGNIAVPLDLpaLFKGYYKEPERTAKAQT 400
Cdd:cd17642  330 IRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFYAKVVDlDTGKTLGPNERGELCVKGPM--IMKGYVNNPEATKALID 407
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 401 GKYYI-TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAG 479
Cdd:cd17642  408 KDGWLhSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLE---AG 484
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 612673656 480 DDTLVKELQQFVKNEVAPYKYPR-EIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd17642  485 KTMTEKEVMDYVASQVSTAKRLRgGVKFVDEVPKGLTGKIDRRKIRE 531
PRK07788 PRK07788
acyl-CoA synthetase; Validated
32-527 1.92e-59

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 205.93  E-value: 1.92e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILyhnteGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIpcseMLRT-- 109
Cdd:PRK07788  63 PDRAALI-----DERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARII----LLNTgf 133
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 110 -----KDLQYRitHGeINAVIALEPFTVEFEKIKE----YDALTKFVIAG--QKDGWISLEAEKENASNKLEMADTTRDD 178
Cdd:PRK07788 134 sgpqlAEVAAR--EG-VKALVYDDEFTDLLSALPPdlgrLRAWGGNPDDDepSGSTDETLDDLIAGSSTAPLPKPPKPGG 210
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 179 MAILsyTSGTTGNPKAVTHCHGWGFAHL-QMAPKHWLCINEDDLVwatAAPGWQKWVWSPFLSVLGSGATaFVFNGRFSP 257
Cdd:PRK07788 211 IVIL--TSGTTGTPKGAPRPEPSPLAPLaGLLSRVPFRAGETTLL---PAPMFHATGWAHLTLAMALGST-VVLRRRFDP 284
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 258 ETYLELLQKYQINVLCCTPTEYRMMAKL--QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTE-STLL 334
Cdd:PRK07788 285 EATLEDIAKHKATALVVVPVMLSRILDLgpEVLAKYDTSSLKIIFVSGSALSPELATRALEAFGPVLYNLYGSTEvAFAT 364
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 335 IGflkdTPQRI----GSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPalFKGYykeperTA---KAQTGKYYITG 407
Cdd:PRK07788 365 IA----TPEDLaeapGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFP--FEGY------TDgrdKQIIDGLLSSG 432
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 408 DLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDtlvKEL 487
Cdd:PRK07788 433 DVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGAALDE---DAI 509
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 612673656 488 QQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAE 527
Cdd:PRK07788 510 KDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREMD 549
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
177-524 2.12e-59

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 200.58  E-value: 2.12e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAVTHCH------GWGFAH-LQMAPKHWLCIneddlvwataaPgwqkwvwSPF----------L 239
Cdd:cd05917    2 DDVINIQFTSGTTGSPKGATLTHhnivnnGYFIGErLGLTEQDRLCI-----------P-------VPLfhcfgsvlgvL 63
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 240 SVLGSGATAfVFNGR-FSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF 318
Cdd:cd05917   64 ACLTHGATM-VFPSPsFDPLAVLEAIEKEKCTALHGVPTMFIAELEHPDFDKFDLSSLRTGIMAGAPCPPELMKRVIEVM 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 319 NLT-VRDGYGQTESTLLI--GFLKDTP-QRIGSMGKGIPGSSVTVVDDEGNSVPA-NTKGNIAVPLDLpaLFKGYYKEPE 393
Cdd:cd05917  143 NMKdVTIAYGMTETSPVStqTRTDDSIeKRVNTVGRIMPHTEAKIVDPEGGIVPPvGVPGELCIRGYS--VMKGYWNDPE 220
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 394 RTAKAQTG-KYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVI 472
Cdd:cd05917  221 KTAEAIDGdGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIR 300
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|..
gi 612673656 473 LQDhtaGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd05917  301 LKE---GAELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
50-526 6.68e-59

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 204.22  E-value: 6.68e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEP 129
Cdd:PRK06155  48 TYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHILRNSGARLLVVEAA 127
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 130 FTVEFEKIKEYD-ALTKFVIAGQKDGWiSLEAEKENAS-----NKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHG--- 200
Cdd:PRK06155 128 LLAALEAADPGDlPLPAVWLLDAPASV-SVPAGWSTAPlppldAPAPAAAVQPGDTAAILYTSGTTGPSKGVCCPHAqfy 206
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 201 -WGfahLQMApkHWLCINEDDlVWATAAPGWQKWVWSPFLSVLGSGATaFVFNGRFSPETYLELLQKYQinvlcCTPTEY 279
Cdd:PRK06155 207 wWG---RNSA--EDLEIGADD-VLYTTLPLFHTNALNAFFQALLAGAT-YVLEPRFSASGFWPAVRRHG-----ATVTYL 274
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 280 --RMMAKL--QNLNDYDLTH-LHSAVSAGEPLnrEVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTpQRIGSMGKGIPG 354
Cdd:PRK06155 275 lgAMVSILlsQPARESDRAHrVRVALGPGVPA--ALHAAFRERFGVDLLDGYGSTETNFVIAVTHGS-QRPGSMGRLAPG 351
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 355 SSVTVVDDEGNSVPANTKGNIAVPLDLP-ALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGY 433
Cdd:PRK06155 352 FEARVVDEHDQELPDGEPGELLLRADEPfAFATGYFGMPEKTVEAWRNLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGE 431
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 434 TIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTlvkELQQFVKNEVAPYKYPREIEFVDDLPKT 513
Cdd:PRK06155 432 NISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLRDGTALEPV---ALVRHCEPRLAYFAVPRYVEFVAALPKT 508
                        490
                 ....*....|...
gi 612673656 514 NSGKIRRVELRDA 526
Cdd:PRK06155 509 ENGKVQKFVLREQ 521
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
30-529 9.55e-59

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 204.24  E-value: 9.55e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  30 HIPDKKAILYHNtegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT 109
Cdd:PRK12583  30 RFPDREALVVRH---QALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRA 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 110 KDLQYRITHGEINAVIALEPFtvefeKIKEYDALTKFVIAGQKDG-----------------------------WISLEA 160
Cdd:PRK12583 107 SELEYALGQSGVRWVICADAF-----KTSDYHAMLQELLPGLAEGqpgalacerlpelrgvvslapapppgflaWHELQA 181
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 161 EKENASNK-LE--MADTTRDDMAILSYTSGTTGNPKAVTHCH------GWgFAHLQMApkhwlCINEDDLVwataapgwq 231
Cdd:PRK12583 182 RGETVSREaLAerQASLDRDDPINIQYTSGTTGFPKGATLSHhnilnnGY-FVAESLG-----LTEHDRLC--------- 246
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 232 kwVWSPF-------LSVLG---SGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYrmMAKLQN--LNDYDLTHLHSA 299
Cdd:PRK12583 247 --VPVPLyhcfgmvLANLGcmtVGACLVYPNEAFDPLATLQAVEEERCTALYGVPTMF--IAELDHpqRGNFDLSSLRTG 322
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 300 VSAGEPLNREVVEQFKKYFNLT-VRDGYGQTES---TLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNI 375
Cdd:PRK12583 323 IMAGAPCPIEVMRRVMDEMHMAeVQIAYGMTETspvSLQTTAADDLERRVETVGRTQPHLEVKVVDPDGATVPRGEIGEL 402
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 376 AVPLDLpaLFKGYYKEPERTAKAQTGKYYI-TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECA 454
Cdd:PRK12583 403 CTRGYS--VMKGYWNNPEATAESIDEDGWMhTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQ 480
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 612673656 455 VVAKPHDIRGNIVKAFVILQ-DHTAGDDtlvkELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIE 529
Cdd:PRK12583 481 VFGVPDEKYGEEIVAWVRLHpGHAASEE----ELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMREISIE 552
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
19-523 1.02e-58

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 202.78  E-value: 1.02e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  19 YNIISEIEKYSHI-PDKKAILyhnteGEDISVTYQQLIEQSNKVGNVLASH-GLSKGDKVLIMMPRSIATYELYIAALKL 96
Cdd:PRK06839   2 QGIAYWIEKRAYLhPDRIAII-----TEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKV 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  97 GVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFViagqkdgWISLEAEKENASnKLEMADTTR 176
Cdd:PRK06839  77 ECIAVPLNIRLTENELIFQLKDSGTTVLFVEKTFQNMALSMQKVSYVQRVI-------SITSLKEIEDRK-IDNFVEKNE 148
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAvthchgwgfAHLQMAPKHWLCINEDDLVWATAAPgwQKWVWSPFLSVLGSGATAF------- 249
Cdd:PRK06839 149 SASFIICYTSGTTGKPKG---------AVLTQENMFWNALNNTFAIDLTMHD--RSIVLLPLFHIGGIGLFAFptlfagg 217
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 250 --VFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYfNLTVRDGYG 327
Cdd:PRK06839 218 viIVPRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETTNLQSVRWFYNGGAPCPEELMREFIDR-GFLFGQGFG 296
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 328 QTES--TLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYI 405
Cdd:PRK06839 297 MTETspTVFMLSEEDARRKVGSIGKPVLFCDYELIDENKNKVEVGEVGELLI--RGPNVMKEYWNRPDATEETIQDGWLC 374
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 406 TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTLVK 485
Cdd:PRK06839 375 TGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKK---SSSVLIEK 451
                        490       500       510
                 ....*....|....*....|....*....|....*...
gi 612673656 486 ELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:PRK06839 452 DVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQL 489
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
30-525 1.16e-58

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 203.75  E-value: 1.16e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  30 HIPDKKAIL-YHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLR 108
Cdd:PRK13295  36 SCPDKTAVTaVRLGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIFR 115
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 TKDLQYRITHGEINAVIALEPFT-VEFEKI-----KEYDALTKFVIAGqKDGWISLEA-----EKENASNKLEMADTTR- 176
Cdd:PRK13295 116 ERELSFMLKHAESKVLVVPKTFRgFDHAAMarrlrPELPALRHVVVVG-GDGADSFEAllitpAWEQEPDAPAILARLRp 194
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 --DDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHwLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAfVFNGR 254
Cdd:PRK13295 195 gpDDVTQLIYTSGTTGEPKGVMHTANTLMANIVPYAER-LGLGADDVILMASPMAHQTGFMYGLMMPVMLGATA-VLQDI 272
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 255 FSPETYLELLQKYQIN-VLCCTP--TEyrmMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTES 331
Cdd:PRK13295 273 WDPARAAELIRTEGVTfTMASTPflTD---LTRAVKESGRPVSSLRTFLCAGAPIPGALVERARAALGAKIVSAWGMTEN 349
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 332 TLL-IGFLKDTPQR-IGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGkYYITGDL 409
Cdd:PRK13295 350 GAVtLTKLDDPDERaSTTDGCPLPGVEVRVVDADGAPLPAGQIGRLQV--RGCSNFGGYLKRPQLNGTDADG-WFDTGDL 426
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 410 AHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTLVKELQQ 489
Cdd:PRK13295 427 ARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPR---PGQSLDFEEMVE 503
                        490       500       510
                 ....*....|....*....|....*....|....*..
gi 612673656 490 FVKNE-VAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK13295 504 FLKAQkVAKQYIPERLVVRDALPRTPSGKIQKFRLRE 540
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
34-525 3.05e-57

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 199.39  E-value: 3.05e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  34 KKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQ 113
Cdd:cd12119   11 DREIVSRTHEGEVHRYTYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIA 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 114 YRITHGEINAVIA-------LEPFTVEFEKIKEYDALTKFVIAGQKD--GWISLEAEKENASNKLEMADTTRDDMAILSY 184
Cdd:cd12119   91 YIINHAEDRVVFVdrdflplLEAIAPRLPTVEHVVVMTDDAAMPEPAgvGVLAYEELLAAESPEYDWPDFDENTAAAICY 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 185 TSGTTGNPKAVTHCHGWGFAH-LQMAPKHWLCINEDDLVWAtAAPGWQKWVW-SPFLSVLgSGAtAFVFNGRF-SPETYL 261
Cdd:cd12119  171 TSGTTGNPKGVVYSHRSLVLHaMAALLTDGLGLSESDVVLP-VVPMFHVNAWgLPYAAAM-VGA-KLVLPGPYlDPASLA 247
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 262 ELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFnLTVRDGYGQTE-------STLL 334
Cdd:cd12119  248 ELIEREGVTFAAGVPTVWQGLLDHLEANGRDLSSLRRVVIGGSAVPRSLIEAFEERG-VRVIHAWGMTEtsplgtvARPP 326
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 335 IGFLKDTPQ-----RIgSMGKGIPGSSVTVVDDEGNSVPA--NTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITG 407
Cdd:cd12119  327 SEHSNLSEDeqlalRA-KQGRPVPGVELRIVDDDGRELPWdgKAVGELQV--RGPWVTKSYYKNDEESEALTEDGWLRTG 403
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 408 DLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVKEL 487
Cdd:cd12119  404 DVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKE---GATVTAEEL 480
                        490       500       510
                 ....*....|....*....|....*....|....*...
gi 612673656 488 QQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd12119  481 LEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALRE 518
PRK07470 PRK07470
acyl-CoA synthetase; Validated
65-526 9.33e-57

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 198.34  E-value: 9.33e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  65 LASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSemlrtkdlqYRITHGEI---------NAVIALEPFTVEFE 135
Cdd:PRK07470  49 LAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTN---------FRQTPDEVaylaeasgaRAMICHADFPEHAA 119
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 136 KIKEYDALTKFVIA-GQKDGWISLEAE-KENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGwgfahlQMA---P 210
Cdd:PRK07470 120 AVRAASPDLTHVVAiGGARAGLDYEALvARHLGARVANAAVDHDDPCWFFFTSGTTGRPKAAVLTHG------QMAfviT 193
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 211 KHwLCinedDLVWATA--------APgwqkwvwspfLSvLGSG-----------ATAFVFNGRFSPETYLELLQKYQINV 271
Cdd:PRK07470 194 NH-LA----DLMPGTTeqdaslvvAP----------LS-HGAGihqlcqvargaATVLLPSERFDPAEVWALVERHRVTN 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 272 LCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNRE-----------VVEQFkkyfnltvrdgYGQTESTLLIGFL-- 338
Cdd:PRK07470 258 LFTVPTILKMLVEHPAVDRYDHSSLRYVIYAGAPMYRAdqkralaklgkVLVQY-----------FGLGEVTGNITVLpp 326
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 339 -----KDTPQ-RIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLdlPALFKGYYKEPERTAKAQTGKYYITGDLAHV 412
Cdd:PRK07470 327 alhdaEDGPDaRIGTCGFERTGMEVQIQDDEGRELPPGETGEICVIG--PAVFAGYYNNPEANAKAFRDGWFRTGDLGHL 404
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 413 DDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDtlvKELQQFVK 492
Cdd:PRK07470 405 DARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDE---AELLAWLD 481
                        490       500       510
                 ....*....|....*....|....*....|....
gi 612673656 493 NEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDA 526
Cdd:PRK07470 482 GKVARYKLPKRFFFWDALPKSGYGKITKKMVREE 515
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
32-526 1.89e-56

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 197.68  E-value: 1.89e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILyhnteGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:COG1021   39 PDRIAVV-----DGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIPVFALPAHRRAE 113
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIA--------LEPFTVEFekIKEYDALTKFVIAGQKDGWISLeAEKENASNKLEMADTTRDDMAILS 183
Cdd:COG1021  114 ISHFAEQSEAVAYIIpdrhrgfdYRALAREL--QAEVPSLRHVLVVGDAGEFTSL-DALLAAPADLSEPRPDPDDVAFFQ 190
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 184 YTSGTTGNPKAV--THcHGWGFAHLQMAPkhwLC-INEDD--LVWATAA-------PGwqkwvwspFLSVLGSGATAfVF 251
Cdd:COG1021  191 LSGGTTGLPKLIprTH-DDYLYSVRASAE---ICgLDADTvyLAALPAAhnfplssPG--------VLGVLYAGGTV-VL 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 252 NGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTES 331
Cdd:COG1021  258 APDPSPDTAFPLIERERVTVTALVPPLALLWLDAAERSRYDLSSLRVLQVGGAKLSPELARRVRPALGCTLQQVFGMAEG 337
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 332 TLLIGFLKDTPQRI-GSMGKGI-PGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQT--GkYYITG 407
Cdd:COG1021  338 LVNYTRLDDPEEVIlTTQGRPIsPDDEVRIVDEDGNPVPPGEVGELLT--RGPYTIRGYYRAPEHNARAFTpdG-FYRTG 414
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 408 DLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGddtlVKEL 487
Cdd:COG1021  415 DLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPRGEPLT----LAEL 490
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 612673656 488 QQFVKN-EVAPYKYPREIEFVDDLPKTNSGKIRRVELRDA 526
Cdd:COG1021  491 RRFLRErGLAAFKLPDRLEFVDALPLTAVGKIDKKALRAA 530
PRK06145 PRK06145
acyl-CoA synthetase; Validated
32-525 1.03e-55

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 194.72  E-value: 1.03e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEgedisVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK06145  16 PDRAALVYRDQE-----ISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADE 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEpftvEFEkikeydaltkfVIAGQKDGWISLEAEKENASNKL--------EMADTTRDDMAILS 183
Cdd:PRK06145  91 VAYILGDAGAKLLLVDE----EFD-----------AIVALETPKIVIDAAAQADSRRLaqggleipPQAAVAPTDLVRLM 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 184 YTSGTTGNPKAVTHCHG---WGFAHLQMApkhwLCINEDDL---------VWATAAPGwqkwvwspfLSVLGSGATAFVF 251
Cdd:PRK06145 156 YTSGTTDRPKGVMHSYGnlhWKSIDHVIA----LGLTASERllvvgplyhVGAFDLPG---------IAVLWVGGTLRIH 222
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 252 NgRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTE 330
Cdd:PRK06145 223 R-EFDPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRDRFDLDSLAWCIGGGEKTPESRIRDFTRVFtRARYIDAYGLTE 301
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 STLLIGFLKDTPQ--RIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAKAQTGKYYITGD 408
Cdd:PRK06145 302 TCSGDTLMEAGREieKIGSTGRALAHVEIRIADGAGRWLPPNMKGEIC--MRGPKVTKGYWKDPEKTAEAFYGDWFRSGD 379
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 409 LAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTLVKELQ 488
Cdd:PRK06145 380 VGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLN---PGATLTLEALD 456
                        490       500       510
                 ....*....|....*....|....*....|....*..
gi 612673656 489 QFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK06145 457 RHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLRD 493
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
50-530 1.61e-55

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 195.66  E-value: 1.61e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASH-GLSKGDKVLIMMPR----SIATYelyiAALKLGVAIIPCSEMLRTKDLQYRITHGEINAV 124
Cdd:PRK08974  50 TFRKLEERSRAFAAYLQNGlGLKKGDRVALMMPNllqyPIALF----GILRAGMIVVNVNPLYTPRELEHQLNDSGAKAI 125
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 125 IALEPFTVEFEKIKEYDALTKFVIAGQKDgwiSLEAEKENASN-------------KLEMA------------------D 173
Cdd:PRK08974 126 VIVSNFAHTLEKVVFKTPVKHVILTRMGD---QLSTAKGTLVNfvvkyikrlvpkyHLPDAisfrsalhkgrrmqyvkpE 202
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 174 TTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMApkhwlcineddlvwataapgwqKWVWSPFLSV------------ 241
Cdd:PRK08974 203 LVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQA----------------------KAAYGPLLHPgkelvvtalply 260
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 242 ------------LGSGATA-FVFNGRFSPETYLELlQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNR 308
Cdd:PRK08974 261 hifaltvncllfIELGGQNlLITNPRDIPGFVKEL-KKYPFTAITGVNTLFNALLNNEEFQELDFSSLKLSVGGGMAVQQ 339
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 309 EVVEQFKKYFNLTVRDGYGQTE-STLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKG 387
Cdd:PRK08974 340 AVAERWVKLTGQYLLEGYGLTEcSPLVSVNPYDLDYYSGSIGLPVPSTEIKLVDDDGNEVPPGEPGELWV--KGPQVMLG 417
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 388 YYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIV 467
Cdd:PRK08974 418 YWQRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAV 497
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 612673656 468 KAFVILQDHTAGDDtlvkELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEK 530
Cdd:PRK08974 498 KIFVVKKDPSLTEE----ELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARAK 556
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
32-520 4.93e-54

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 189.00  E-value: 4.93e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcsemlrtkd 111
Cdd:cd05945    5 PDRPAVVE-----GGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVP--------- 70
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 lqyrithgeINAVIALEpftvEFEKIKEYDALTKFVIAGqkdgwisleaekenasnklemadttrDDMAILSYTSGTTGN 191
Cdd:cd05945   71 ---------LDASSPAE----RIREILDAAKPALLIADG--------------------------DDNAYIIFTSGSTGR 111
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHG-------WGFAHLQMapkhwlciNEDDLVWATAapgwqkwvwsPF---LSV------LGSGATAFVfNGRF 255
Cdd:cd05945  112 PKGVQISHDnlvsftnWMLSDFPL--------GPGDVFLNQA----------PFsfdLSVmdlypaLASGATLVP-VPRD 172
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 256 SPETYLEL---LQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTES 331
Cdd:cd05945  173 ATADPKQLfrfLAEHGITVWVSTPSFAAMCLLSPTFTPESLPSLRHFLFCGEVLPHKTARALQQRFpDARIYNTYGPTEA 252
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 332 TLLIGFLKDTPQRIGSM-----GKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKA--QTGKY- 403
Cdd:cd05945  253 TVAVTYIEVTPEVLDGYdrlpiGYAKPGAKLVILDEDGRPVPPGEKGELVI--SGPSVSKGYLNNPEKTAAAffPDEGQr 330
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 -YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTagDDT 482
Cdd:cd05945  331 aYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPGA--EAG 408
                        490       500       510
                 ....*....|....*....|....*....|....*...
gi 612673656 483 LVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:cd05945  409 LTKAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDR 446
PRK07798 PRK07798
acyl-CoA synthetase; Validated
31-517 1.27e-53

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 189.71  E-value: 1.27e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  31 IPDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTK 110
Cdd:PRK07798  16 VPDRVALVC-----GDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVED 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 111 DLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVI-------AGQKDGWISLEAEKENASNKLEMADTTRDDMAILs 183
Cdd:PRK07798  91 ELRYLLDDSDAVALVYEREFAPRVAEVLPRLPKLRTLVvvedgsgNDLLPGAVDYEDALAAGSPERDFGERSPDDLYLL- 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 184 YTSGTTGNPKAVTHCHG--W-----GFAHLQMAPKHwlciNEDDLVWATAAPGWQKWV-----------WSPFLSVLGSG 245
Cdd:PRK07798 170 YTGGTTGMPKGVMWRQEdiFrvllgGRDFATGEPIE----DEEELAKRAAAGPGMRRFpapplmhgagqWAAFAALFSGQ 245
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 246 ATAFVFNGRFSPETYLELLQKYQINVLCCT------PteyrMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF- 318
Cdd:PRK07798 246 TVVLLPDVRFDADEVWRTIEREKVNVITIVgdamarP----LLDALEARGPYDLSSLFAIASGGALFSPSVKEALLELLp 321
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 319 NLTVRDGYGQTEStlliGFLKDTPQRIGSMGKGIP----GSSVTVVDDEGNSVPANT--KGNIA----VPLdlpalfkGY 388
Cdd:PRK07798 322 NVVLTDSIGSSET----GFGGSGTVAKGAVHTGGPrftiGPRTVVLDEDGNPVEPGSgeIGWIArrghIPL-------GY 390
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 389 YKEPERTAKaqTGK------YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDI 462
Cdd:PRK07798 391 YKDPEKTAE--TFPtidgvrYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDER 468
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 612673656 463 RGNIVKAFVILQDHTAGDDTlvkELQQFVKNEVAPYKYPREIEFVDDLPKTNSGK 517
Cdd:PRK07798 469 WGQEVVAVVQLREGARPDLA---ELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
68-524 3.50e-53

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 186.88  E-value: 3.50e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  68 HGLSKGDKVLIMMPRSIATYELYI----AALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVEFEKIkeydal 143
Cdd:cd05922   13 AGGVRGERVVLILPNRFTYIELSFavayAGGRLGLVFVPLNPTLKESVLRYLVADAGGRIVLADAGAADRLRDA------ 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 144 tkFVIAGQKDGWISLEAEKENASNkLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQmAPKHWLCINEDDLVw 223
Cdd:cd05922   87 --LPASPDPGTVLDADGIRAARAS-APAHEVSHEDLALLLYTSGSTGSPKLVRLSHQNLLANAR-SIAEYLGITADDRA- 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 224 ATAAPgwqkWVWSPFLSVLGS----GATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLqNLNDYDLTHLHSA 299
Cdd:cd05922  162 LTVLP----LSYDYGLSVLNThllrGATLVLTNDGVLDDAFWEDLREHGATGLAGVPSTYAMLTRL-GFDPAKLPSLRYL 236
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 300 VSAGEPLNREVVEQFKKYFNLT-VRDGYGQTESTLLIGFLKdtPQRI----GSMGKGIPGSSVTVVDDEGNSVPANTKGN 374
Cdd:cd05922  237 TQAGGRLPQETIARLRELLPGAqVYVMYGQTEATRRMTYLP--PERIlekpGSIGLAIPGGEFEILDDDGTPTPPGEPGE 314
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 375 IAVplDLPALFKGYYK-EPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKEC 453
Cdd:cd05922  315 IVH--RGPNVMKGYWNdPPYRRKEGRGGGVLHTGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEA 392
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 612673656 454 AVVAKPhDIRGNIVKAFVilqdhTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd05922  393 AAVGLP-DPLGEKLALFV-----TAPDKIDPKDVLRSLAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
32-534 1.97e-52

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 186.88  E-value: 1.97e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILyhntEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK06087  37 PDKIAVV----DNHGASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAE 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPF-TVEFEK-----IKEYDALTKFVI---AGQKDGWISLEAEKENASNKLEMADTTRDDMAIL 182
Cdd:PRK06087 113 LVWVLNKCQAKMFFAPTLFkQTRPVDlilplQNQLPQLQQIVGvdkLAPATSSLSLSQIIADYEPLTTAITTHGDELAAV 192
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 183 SYTSGTTGNPKAVTHCHGwgfaHLQMAPKHW---LCINEDDLVWATA----APGWQKWVWSPFLSvlgsGATAfVFNGRF 255
Cdd:PRK06087 193 LFTSGTEGLPKGVMLTHN----NILASERAYcarLNLTWQDVFMMPAplghATGFLHGVTAPFLI----GARS-VLLDIF 263
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 256 SPETYLELLQKYQIN-VLCCTPTEYRMMAKLQNlNDYDLTHLHSAVSAGEPLNREVVEQFKKYfNLTVRDGYGQTEST-- 332
Cdd:PRK06087 264 TPDACLALLEQQRCTcMLGATPFIYDLLNLLEK-QPADLSALRFFLCGGTTIPKKVARECQQR-GIKLLSVYGSTESSph 341
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 333 LLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKA--QTGKYYiTGDLA 410
Cdd:PRK06087 342 AVVNLDDPLSRFMHTDGYAAAGVEIKVVDEARKTLPPGCEGEEAS--RGPNVFMGYLDEPELTARAldEEGWYY-SGDLC 418
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 411 HVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAgDDTLVKELQQF 490
Cdd:PRK06087 419 RMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHH-SLTLEEVVAFF 497
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 612673656 491 VKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEKWQQQ 534
Cdd:PRK06087 498 SRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRKDIMRRLTQD 541
PLN02654 PLN02654
acetate-CoA ligase
33-524 1.07e-51

acetate-CoA ligase


Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 187.03  E-value: 1.07e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  33 DKKAILYH-NTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPrsiatyelyiAALKLGVAIIPCSEM----- 106
Cdd:PLN02654 104 DKIAIYWEgNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLP----------MLMELPIAMLACARIgavhs 173
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 107 -----LRTKDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGW---ISLEAEKENASNK---------- 168
Cdd:PLN02654 174 vvfagFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVsvgICLTYENQLAMKRedtkwqegrd 253
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 169 ---------------LEMADTtrDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGW--- 230
Cdd:PLN02654 254 vwwqdvvpnyptkceVEWVDA--EDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVYWCTADCGWitg 331
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 231 QKWV-WSPFLSvlgsGATAFVFNGRFS---PETYLELLQKYQINVLCCTPTEYR--MMAKLQNLNDYDLTHLHSAVSAGE 304
Cdd:PLN02654 332 HSYVtYGPMLN----GATVLVFEGAPNypdSGRCWDIVDKYKVTIFYTAPTLVRslMRDGDEYVTRHSRKSLRVLGSVGE 407
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 305 PLNREVveqFKKYFNLT------VRDGYGQTESTlliGFLKDT-----PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKG 373
Cdd:PLN02654 408 PINPSA---WRWFFNVVgdsrcpISDTWWQTETG---GFMITPlpgawPQKPGSATFPFFGVQPVIVDEKGKEIEGECSG 481
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 374 NIAVPLDLPALFKGYYKEPER---TAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAV 450
Cdd:PLN02654 482 YLCVKKSWPGAFRTLYGDHERyetTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQC 561
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 612673656 451 KECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PLN02654 562 AEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 635
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
23-526 2.11e-50

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 181.61  E-value: 2.11e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  23 SEIEKYSHIPdkkaiLYHNTeGEDIsvTYQ---QLIEQSnkVGNVLASHGLSKGDKVLIMMPR----SIATYelyiAALK 95
Cdd:PRK08751  33 TSVAKFADRP-----AYHSF-GKTI--TYReadQLVEQF--AAYLLGELQLKKGDRVALMMPNclqyPIATF----GVLR 98
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  96 LGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVEFEK------IKEY-------------DALTKFVIAGQK---- 152
Cdd:PRK08751  99 AGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQviadtpVKQVittglgdmlgfpkAALVNFVVKYVKklvp 178
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 153 ----DGWISL-EAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMApKHWLC----INEDDLVW 223
Cdd:PRK08751 179 eyriNGAIRFrEALALGRKHSMPTLQIEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQA-HQWLAgtgkLEEGCEVV 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 224 ATAAPGWQKWVWSP----FLSVlgSGATAFVFNGRFSPeTYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSA 299
Cdd:PRK08751 258 ITALPLYHIFALTAnglvFMKI--GGCNHLISNPRDMP-GFVKELKKTRFTAFTGVNTLFNGLLNTPGFDQIDFSSLKMT 334
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 300 VSAGEPLNREVVEQFKKYFNLTVRDGYGQTEST--LLIGFLkDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAV 377
Cdd:PRK08751 335 LGGGMAVQRSVAERWKQVTGLTLVEAYGLTETSpaACINPL-TLKEYNGSIGLPIPSTDACIKDDAGTVLAIGEIGELCI 413
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 378 plDLPALFKGYYKEPERTAKAQTGKYYI-TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVV 456
Cdd:PRK08751 414 --KGPQVMKGYWKRPEETAKVMDADGWLhTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAV 491
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 612673656 457 AKPHDIRGNIVKAFVILQDH--TAgddtlvKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDA 526
Cdd:PRK08751 492 GVPDEKSGEIVKVVIVKKDPalTA------EDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRRELRDA 557
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
182-525 4.64e-50

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 178.73  E-value: 4.64e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 182 LSYTSGTTGNPKAVTHCHGWGFAH--LQMAPKHWLCINEDDlVWATAAPGWQKWVWSPFLSVLGSGATAFVFNgRFSPET 259
Cdd:cd05929  130 MLYSGGTTGRPKGIKRGLPGGPPDndTLMAAALGFGPGADS-VYLSPAPLYHAAPFRWSMTALFMGGTLVLME-KFDPEE 207
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 260 YLELLQKYQINVLCCTPTEYRMMAKLQN--LNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTE---STLL 334
Cdd:cd05929  208 FLRLIERYRVTFAQFVPTMFVRLLKLPEavRNAYDLSSLKRVIHAAAPCPPWVKEQWIDWGGPIIWEYYGGTEgqgLTII 287
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 335 IG--FLKDTpqriGSMGKGIpGSSVTVVDDEGNSVPANTKGNIAVpldLPALFKGYYKEPERTAKAQTGKYYIT-GDLAH 411
Cdd:cd05929  288 NGeeWLTHP----GSVGRAV-LGKVHILDEDGNEVPPGEIGEVYF---ANGPGFEYTNDPEKTAAARNEGGWSTlGDVGY 359
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 412 VDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFV 491
Cdd:cd05929  360 LDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPGADAGTALAEELIAFL 439
                        330       340       350
                 ....*....|....*....|....*....|....
gi 612673656 492 KNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd05929  440 RDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLRD 473
PRK13382 PRK13382
bile acid CoA ligase;
32-525 4.77e-50

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 180.34  E-value: 4.77e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILyhnteGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK13382  57 PDRPGLI-----DELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPA 131
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFTVEFEK-IKEYDALTKFVIAGQKDGWISLEAEKENASNKLEMADTTRDDMAILsyTSGTTG 190
Cdd:PRK13382 132 LAEVVTREGVDTVIYDEEFSATVDRaLADCPQATRIVAWTDEDHDLTVEVLIAAHAGQRPEPTGRKGRVILL--TSGTTG 209
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 191 NPKAVTHCHGWGFAHLQ--MAPKHWLCinEDDLVwaTAAPGWQKWVWSPfLSVLGSGATAFVFNGRFSPETYLELLQKYQ 268
Cdd:PRK13382 210 TPKGARRSGPGGIGTLKaiLDRTPWRA--EEPTV--IVAPMFHAWGFSQ-LVLAASLACTIVTRRRFDPEATLDLIDRHR 284
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 269 INVLCCTPTEYRMMAKL--QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLL-IGFLKDTPQRI 345
Cdd:PRK13382 285 ATGLAVVPVMFDRIMDLpaEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEAGMIaTATPADLRAAP 364
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 346 GSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLpaLFKGYykEPERTAKAQTGkYYITGDLAHVDDDGYFWFEGRRD 425
Cdd:PRK13382 365 DTAGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDT--QFDGY--TSGSTKDFHDG-FMASGDVGYLDENGRLFVVGRDD 439
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 426 DIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILqdhTAGDDTLVKELQQFVKNEVAPYKYPREIE 505
Cdd:PRK13382 440 EMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVL---KPGASATPETLKQHVRDNLANYKVPRDIV 516
                        490       500
                 ....*....|....*....|
gi 612673656 506 FVDDLPKTNSGKIRRVELRD 525
Cdd:PRK13382 517 VLDELPRGATGKILRRELQA 536
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
32-523 5.87e-50

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 178.68  E-value: 5.87e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILyhnteGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd05920   29 PDRIAVV-----DGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLALPSHRRSE 103
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIalepftvefekikeydaltkfvIAGQKDGWISLEAEKENASNKlemadttrDDMAILSYTSGTTGN 191
Cdd:cd05920  104 LSAFCAHAEAVAYI----------------------VPDRHAGFDHRALARELAESI--------PEVALFLLSGGTTGT 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGwGFAHLQMAPKHWLCINEDDlVWATAAPGWQKWVWS-P-FLSVLGSGATAfVFNGRFSPETYLELLQKYQI 269
Cdd:cd05920  154 PKLIPRTHN-DYAYNVRASAEVCGLDQDT-VYLAVLPAAHNFPLAcPgVLGTLLAGGRV-VLAPDPSPDAAFPLIEREGV 230
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 270 NVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRI-GSM 348
Cdd:cd05920  231 TVTALVPALVSLWLDAAASRRADLSSLRLLQVGGARLSPALARRVPPVLGCTLQQVFGMAEGLLNYTRLDDPDEVIiHTQ 310
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 349 GKGI-PGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGK-YYITGDLAHVDDDGYFWFEGRRDD 426
Cdd:cd05920  311 GRPMsPDDEIRVVDEEGNPVPPGEEGELLT--RGPYTIRGYYRAPEHNARAFTPDgFYRTGDLVRRTPDGYLVVEGRIKD 388
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 427 IIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGddtlVKELQQFVKN-EVAPYKYPREIE 505
Cdd:cd05920  389 QINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVLRDPPPS----AAQLRRFLRErGLAAYKLPDRIE 464
                        490
                 ....*....|....*...
gi 612673656 506 FVDDLPKTNSGKIRRVEL 523
Cdd:cd05920  465 FVDSLPLTAVGKIDKKAL 482
PRK09088 PRK09088
acyl-CoA synthetase; Validated
50-526 1.54e-49

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 177.69  E-value: 1.54e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcsemlrtkdLQYRITHGEINAVIA-LE 128
Cdd:PRK09088  24 TYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVP---------LNWRLSASELDALLQdAE 94
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 129 PftvefeKIKEYDALtkfVIAGQKDGwISLEAEKENAsNKLEMADTT---RDDMAILSYTSGTTGNPKAVTHCHgwgfAH 205
Cdd:PRK09088  95 P------RLLLGDDA---VAAGRTDV-EDLAAFIASA-DALEPADTPsipPERVSLILFTSGTSGQPKGVMLSE----RN 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 206 LQMApkhwlCINEDDLVWATAApgwqkwvwSPFL----------------SVLGSGATAFVFNGrFSPETYLELLQKYQI 269
Cdd:PRK09088 160 LQQT-----AHNFGVLGRVDAH--------SSFLcdapmfhiiglitsvrPVLAVGGSILVSNG-FEPKRTLGRLGDPAL 225
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 270 NV--LCCTPteyRMMAKLQNLNDYD---LTHLHSAVSAGEPlnrEVVEQFKKYFNLTVR--DGYGQTESTLLIGFLKDTP 342
Cdd:PRK09088 226 GIthYFCVP---QMAQAFRAQPGFDaaaLRHLTALFTGGAP---HAAEDILGWLDDGIPmvDGFGMSEAGTVFGMSVDCD 299
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 343 ---QRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAKAQTGK-YYITGDLAHVDDDGYF 418
Cdd:PRK09088 300 virAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELL--LRGPNLSPGYWRRPQATARAFTGDgWFRTGDIARRDADGFF 377
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 419 WFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVIlqdHTAGDDTLVKELQQFVKNEVAPY 498
Cdd:PRK09088 378 WVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIV---PADGAPLDLERIRSHLSTRLAKY 454
                        490       500
                 ....*....|....*....|....*...
gi 612673656 499 KYPREIEFVDDLPKTNSGKIRRVELRDA 526
Cdd:PRK09088 455 KVPKHLRLVDALPRTASGKLQKARLRDA 482
PRK08162 PRK08162
acyl-CoA synthetase; Validated
32-526 2.15e-49

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 178.60  E-value: 2.15e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK08162  32 PDRPAVIH-----GDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAAS 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFT-VEFEKIKEYDALTKFVI--------AGQKDGwiSLEAEKENASNKLEMADTTRDDM--A 180
Cdd:PRK08162 107 IAFMLRHGEAKVLIVDTEFAeVAREALALLPGPKPLVIdvddpeypGGRFIG--ALDYEAFLASGDPDFAWTLPADEwdA 184
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 181 I-LSYTSGTTGNPKAVT--------------------------------HCHGWGFAhlqmapkhwlcineddlvWATAA 227
Cdd:PRK08162 185 IaLNYTSGTTGNPKGVVyhhrgaylnalsnilawgmpkhpvylwtlpmfHCNGWCFP------------------WTVAA 246
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 228 pgwqkwvwspflsvlgSGATAfVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLN 307
Cdd:PRK08162 247 ----------------RAGTN-VCLRKVDPKLIFDLIREHGVTHYCGAPIVLSALINAPAEWRAGIDHPVHAMVAGAAPP 309
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 308 REVVEQFKKY-FNLT-VrdgYGQTE--------------STLLIgflkdtPQRIGSMGK-GIP---GSSVTVVD-DEGNS 366
Cdd:PRK08162 310 AAVIAKMEEIgFDLThV---YGLTEtygpatvcawqpewDALPL------DERAQLKARqGVRyplQEGVTVLDpDTMQP 380
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 367 VPAN--TKGNIAVPLDLpaLFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDAL 444
Cdd:PRK08162 381 VPADgeTIGEIMFRGNI--VMKGYLKNPKATEEAFAGGWFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVL 458
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 445 TNHPAVKECAVVAKPHDIRGNIVKAFVILQD-HTAGDDtlvkELQQFVKNEVAPYKYPREIEFvDDLPKTNSGKIRRVEL 523
Cdd:PRK08162 459 YRHPAVLVAAVVAKPDPKWGEVPCAFVELKDgASATEE----EIIAHCREHLAGFKVPKAVVF-GELPKTSTGKIQKFVL 533

                 ...
gi 612673656 524 RDA 526
Cdd:PRK08162 534 REQ 536
PRK08315 PRK08315
AMP-binding domain protein; Validated
32-536 9.69e-49

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 176.93  E-value: 9.69e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTegeDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK08315  30 PDREALVYRDQ---GLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVTINPAYRLSE 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPF----------TVEFE-KIKEYDALT-------KFVI-AGQK-----DGWISLEAEKENASN 167
Cdd:PRK08315 107 LEYALNQSGCKALIAADGFkdsdyvamlyELAPElATCEPGQLQsarlpelRRVIfLGDEkhpgmLNFDELLALGRAVDD 186
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 168 -KLE--MADTTRDDMAILSYTSGTTGNPKAVTHCH------GWGFA-HLQMAPKHWLCIneddlvwataaPgwqkwVwsP 237
Cdd:PRK08315 187 aELAarQATLDPDDPINIQYTSGTTGFPKGATLTHrnilnnGYFIGeAMKLTEEDRLCI-----------P-----V--P 248
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 238 F-------LSVLG---SGATAFVFNGRFSPETYLELLQKYQinvlcCT-----PTeyrM-MAKLQ--NLNDYDLTHLHSA 299
Cdd:PRK08315 249 LyhcfgmvLGNLAcvtHGATMVYPGEGFDPLATLAAVEEER-----CTalygvPT---MfIAELDhpDFARFDLSSLRTG 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 300 VSAGEPLNREVVEQFKKYFNLT-VRDGYGQTES------TLLigflkDTP--QRIGSMGKGIPGSSVTVVDDE-GNSVPA 369
Cdd:PRK08315 321 IMAGSPCPIEVMKRVIDKMHMSeVTIAYGMTETspvstqTRT-----DDPleKRVTTVGRALPHLEVKIVDPEtGETVPR 395
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 370 NTKGNI-----AVpldlpalFKGYYKEPERTAKAqtgkyyI-------TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGP 437
Cdd:PRK08315 396 GEQGELctrgySV-------MKGYWNDPEKTAEA------IdadgwmhTGDLAVMDEEGYVNIVGRIKDMIIRGGENIYP 462
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 438 FEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQ-DHTAGDDtlvkELQQFVKNEVAPYKYPREIEFVDDLPKTNSG 516
Cdd:PRK08315 463 REIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRpGATLTEE----DVRDFCRGKIAHYKIPRYIRFVDEFPMTVTG 538
                        570       580
                 ....*....|....*....|
gi 612673656 517 KIRRVELRDAEIEKWQQQKD 536
Cdd:PRK08315 539 KIQKFKMREMMIEELGLQAA 558
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
32-523 1.27e-48

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 174.42  E-value: 1.27e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCsemlrtkD 111
Cdd:cd17643    1 PEAVAVVD-----EDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPI-------D 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQY---RITHgeinavialepfTVEfekikeyDALTKFVIagqkdgwisleaekenasnklemadTTRDDMAILSYTSGT 188
Cdd:cd17643   69 PAYpveRIAF------------ILA-------DSGPSLLL-------------------------TDPDDLAYVIYTSGS 104
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 189 TGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNG-RFSPETYLELLQKY 267
Cdd:cd17643  105 TGRPKGVVVSHA-NVLALFAATQRWFGFNEDDVWTLFHSYAFDFSVWEIWGALLHGGRLVVVPYEvARSPEDFARLLRDE 183
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 268 QINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNL---TVRDGYGQTESTLLIGFLK----D 340
Cdd:cd17643  184 GVTVLNQTPSAFYQLVEAADRDGRDPLALRYVIFGGEALEAAMLRPWAGRFGLdrpQLVNMYGITETTVHVTFRPldaaD 263
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 341 TPQRIGS-MGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAK--------AQTGKYYITGDLAH 411
Cdd:cd17643  264 LPAAAASpIGRPLPGLRVYVLDADGRPVPPGVVGELYV--SGAGVARGYLGRPELTAErfvanpfgGPGSRMYRTGDLAR 341
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 412 VDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDdtlVKELQQFV 491
Cdd:cd17643  342 RLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAAAD---IAELRALL 418
                        490       500       510
                 ....*....|....*....|....*....|..
gi 612673656 492 KNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd17643  419 KELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
PRK07529 PRK07529
AMP-binding domain protein; Validated
32-529 2.25e-48

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 177.45  E-value: 2.25e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEGED---ISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPrsiATYELYIAALKLGVAIIPC----- 103
Cdd:PRK07529  39 PDAPALSFLLDADPLdrpETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLP---NLPETHFALWGGEAAGIANpinpl 115
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 104 ------SEMLRTKDLQyrithgeinAVIALEPF--TVEFEKIKEY-DAL----TKFVIAGQKD-----GWISLEAEKE-- 163
Cdd:PRK07529 116 lepeqiAELLRAAGAK---------VLVTLGPFpgTDIWQKVAEVlAALpelrTVVEVDLARYlpgpkRLAVPLIRRKah 186
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 164 ------NASNKLEMADTTR-------DDMAILSYTSGTTGNPKAVTHCHGwgfahlQMAPKHW-----LCINEDDLVWAt 225
Cdd:PRK07529 187 arildfDAELARQPGDRLFsgrpigpDDVAAYFHTGGTTGMPKLAQHTHG------NEVANAWlgallLGLGPGDTVFC- 259
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 226 AAP-----GWQKWVWSPFLS----VLGSGA----TAFVFNgrfspetYLELLQKYQINVLCCTPTEYRMMAKLQnLNDYD 292
Cdd:PRK07529 260 GLPlfhvnALLVTGLAPLARgahvVLATPQgyrgPGVIAN-------FWKIVERYRINFLSGVPTVYAALLQVP-VDGHD 331
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 293 LTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLL--IGFLkDTPQRIGSMGKGIPGSSVTVV--DDEGNSV- 367
Cdd:PRK07529 332 ISSLRYALCGAAPLPVEVFRRFEAATGVRIVEGYGLTEATCVssVNPP-DGERRIGSVGLRLPYQRVRVVilDDAGRYLr 410
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 368 --PANTKGNIAVplDLPALFKGYYkEPERTAKAQT-GKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDAL 444
Cdd:PRK07529 411 dcAVDEVGVLCI--AGPNVFSGYL-EAAHNKGLWLeDGWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEAL 487
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 445 TNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTLVKELQQFVKNEVA-PYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:PRK07529 488 LRHPAVALAAAVGRPDAHAGELPVAYVQLK---PGASATEAELLAFARDHIAeRAAVPKHVRILDALPKTAVGKIFKPAL 564

                 ....*.
gi 612673656 524 RDAEIE 529
Cdd:PRK07529 565 RRDAIR 570
PRK07514 PRK07514
malonyl-CoA synthase; Validated
29-526 3.15e-48

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 174.68  E-value: 3.15e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  29 SHIPDKKAILYHNTEGEDIsvTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLR 108
Cdd:PRK07514  11 AAFADRDAPFIETPDGLRY--TYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYT 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 TKDLQYRITHGEiNAVIALEPftvefekiKEYDALTKfvIAGQK----------DGWISLEAEKENASNKLEMADTTRDD 178
Cdd:PRK07514  89 LAELDYFIGDAE-PALVVCDP--------ANFAWLSK--IAAAAgaphvetldaDGTGSLLEAAAAAPDDFETVPRGADD 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 179 MAILSYTSGTTGNPKAVTHCHG------------WGFahlqmapkhwlciNEDDlVWATAAPGWQkwVWSPFLS---VLG 243
Cdd:PRK07514 158 LAAILYTSGTTGRSKGAMLSHGnllsnaltlvdyWRF-------------TPDD-VLIHALPIFH--THGLFVAtnvALL 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 244 SGAtAFVFNGRFSPETYLELLQkyQINVLCCTPTEY-RMMAKlQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTV 322
Cdd:PRK07514 222 AGA-SMIFLPKFDPDAVLALMP--RATVMMGVPTFYtRLLQE-PRLTREAAAHMRLFISGSAPLLAETHREFQERTGHAI 297
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 323 RDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVD-DEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTG 401
Cdd:PRK07514 298 LERYGMTETNMNTSNPYDGERRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMIEV--KGPNVFKGYWRMPEKTAEEFRA 375
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 402 K-YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGD 480
Cdd:PRK07514 376 DgFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEGVTAVVVPKPGAALD 455
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 612673656 481 -DTLVKELqqfvKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDA 526
Cdd:PRK07514 456 eAAILAAL----KGRLARFKQPKRVFFVDELPRNTMGKVQKNLLREQ 498
PRK07787 PRK07787
acyl-CoA synthetase; Validated
177-524 5.46e-48

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 173.25  E-value: 5.46e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAVthchgwgfahlqMAPKHWLCINEDDLVWAtaapgwqkWVWSP----------------FLS 240
Cdd:PRK07787 128 DAPALIVYTSGTTGPPKGV------------VLSRRAIAADLDALAEA--------WQWTAddvlvhglplfhvhglVLG 187
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 241 VLGS---GATaFVFNGRFSPETYLELLQKyQINVLCCTPTEYRMMAklqnlNDYDLTH-LHSA---VSAGEPLNREVVEQ 313
Cdd:PRK07787 188 VLGPlriGNR-FVHTGRPTPEAYAQALSE-GGTLYFGVPTVWSRIA-----ADPEAARaLRGArllVSGSAALPVPVFDR 260
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 314 FKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPAN--TKGNIAVplDLPALFKGYYKE 391
Cdd:PRK07787 261 LAALTGHRPVERYGMTETLITLSTRADGERRPGWVGLPLAGVETRLVDEDGGPVPHDgeTVGELQV--RGPTLFDGYLNR 338
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 392 PERTAKAQTGK-YYITGDLAHVDDDGYFWFEGRRD-DIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKA 469
Cdd:PRK07787 339 PDATAAAFTADgWFRTGDVAVVDPDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVA 418
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 612673656 470 FVIlqdhtAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK07787 419 YVV-----GADDVAADELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
29-523 5.96e-48

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 173.46  E-value: 5.96e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  29 SHIPDKKAILyhnTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLR 108
Cdd:cd05923   12 SRAPDACAIA---DPARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLK 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 TKDLQYRITHGEINAVIalepFTVEFEKIKE-YDALTKFVIAGQKDGwislEAEKENASNKLEMADTTRDDMAILSYTSG 187
Cdd:cd05923   89 AAELAELIERGEMTAAV----IAVDAQVMDAiFQSGVRVLALSDLVG----LGEPESAGPLIEDPPREPEQPAFVFYTSG 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 188 TTGNPKAVthchgwGFAHLQMAPKHwlcineddLVWATAAP---GWQKWVWS--PFLSVLGSGAT---AFVFNGR----- 254
Cdd:cd05923  161 TTGLPKGA------VIPQRAAESRV--------LFMSTQAGlrhGRHNVVLGlmPLYHVIGFFAVlvaALALDGTyvvve 226
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 255 -FSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEStl 333
Cdd:cd05923  227 eFDPADALKLIEQERVTSLFATPTHLDALAAAAEFAGLKLSSLRHVTFAGATMPDAVLERVNQHLPGEKVNIYGTTEA-- 304
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 lIGFLKDTPQRIGSMGKgiPG--SSVTVVDDEGNSV---PANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGD 408
Cdd:cd05923  305 -MNSLYMRDARTGTEMR--PGffSEVRIVRIGGSPDealANGEEGELIVAAAADAAFTGYLNQPEATAKKLQDGWYRTGD 381
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 409 LAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDtlvkELQ 488
Cdd:cd05923  382 VGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREGTLSAD----ELD 457
                        490       500       510
                 ....*....|....*....|....*....|....*.
gi 612673656 489 QFVK-NEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd05923  458 QFCRaSELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
168-534 7.20e-48

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 174.95  E-value: 7.20e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 168 KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAH-LQMAPKHWLCINEDDLVWATAAPGWQKWVWS---PFLSVLG 243
Cdd:PRK05677 198 PVTEANPQADDVAVLQYTGGTTGVAKGAMLTHRNLVANmLQCRALMGSNLNEGCEILIAPLPLYHIYAFTfhcMAMMLIG 277
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 244 sGATAFVFNGRFSPeTYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVR 323
Cdd:PRK05677 278 -NHNILISNPRDLP-AMVKELGKWKFSGFVGLNTLFVALCNNEAFRKLDFSALKLTLSGGMALQLATAERWKEVTGCAIC 355
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 324 DGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKY 403
Cdd:PRK05677 356 EGYGMTETSPVVSVNPSQAIQVGTIGIPVPSTLCKVIDDDGNELPLGEVGELCV--KGPQVMKGYWQRPEATDEILDSDG 433
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 YI-TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtagDDT 482
Cdd:PRK05677 434 WLkTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKP----GET 509
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|...
gi 612673656 483 LVKE-LQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEKWQQQ 534
Cdd:PRK05677 510 LTKEqVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELRDEELKKAGLK 562
prpE PRK10524
propionyl-CoA synthetase; Provisional
32-520 1.19e-47

propionyl-CoA synthetase; Provisional


Pssm-ID: 182517 [Multi-domain]  Cd Length: 629  Bit Score: 175.14  E-value: 1.19e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTE-GEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRsIATyelyiAALklgvAIIPCSemlrtk 110
Cdd:PRK10524  67 PEQLALIAVSTEtDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPM-IAE-----AAF----AMLACA------ 130
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 111 dlqyRIthGEINAVI----ALEPFTVEFEkikeyDALTKFVI---AGQKDGWIS-----LEAEKENASNK---------- 168
Cdd:PRK10524 131 ----RI--GAIHSVVfggfASHSLAARID-----DAKPVLIVsadAGSRGGKVVpykplLDEAIALAQHKprhvllvdrg 199
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 169 -LEMADTT-RD-DMAILS----------------------YTSGTTGNPKAVTHCHGwGFA-HLQMAPKHWLCINEDDLV 222
Cdd:PRK10524 200 lAPMARVAgRDvDYATLRaqhlgarvpvewlesnepsyilYTSGTTGKPKGVQRDTG-GYAvALATSMDTIFGGKAGETF 278
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 223 WATAAPGW----QKWVWSPFLSvlgsGATAFVFNG---RFSPETYLELLQKYQINVLCCTPTEYRMMAKlQN---LNDYD 292
Cdd:PRK10524 279 FCASDIGWvvghSYIVYAPLLA----GMATIMYEGlptRPDAGIWWRIVEKYKVNRMFSAPTAIRVLKK-QDpalLRKHD 353
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 293 LTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTES-----TLLIGfLKDTPQRIGSMGKGIPGSSVTVVDDE-GNS 366
Cdd:PRK10524 354 LSSLRALFLAGEPLDEPTASWISEALGVPVIDNYWQTETgwpilAIARG-VEDRPTRLGSPGVPMYGYNVKLLNEVtGEP 432
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 367 VPANTKGNIAVPLDLP-----------ALF-KGYYKEPERTAkaqtgkyYITGDLAHVDDDGYFWFEGRRDDIIISSGYT 434
Cdd:PRK10524 433 CGPNEKGVLVIEGPLPpgcmqtvwgddDRFvKTYWSLFGRQV-------YSTFDWGIRDADGYYFILGRTDDVINVAGHR 505
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 435 IGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDT-----LVKELQQFVKNEVAPYKYPREIEFVDD 509
Cdd:PRK10524 506 LGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDSLADRearlaLEKEIMALVDSQLGAVARPARVWFVSA 585
                        570
                 ....*....|.
gi 612673656 510 LPKTNSGKIRR 520
Cdd:PRK10524 586 LPKTRSGKLLR 596
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
50-530 1.90e-47

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 173.65  E-value: 1.90e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRS----IATYelyiAALKLGVAIIPCSEMLRTKDLQYRIT-HG----- 119
Cdd:PRK05605  59 TYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCpqhiVAFY----AVLRLGAVVVEHNPLYTAHELEHPFEdHGarvai 134
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 120 ---EINAVIALEPFTVEFEKI-------------------------KEYDALTKFviAGQKDGWISLEAEKENASNKL-E 170
Cdd:PRK05605 135 vwdKVAPTVERLRRTTPLETIvsvnmiaampllqrlalrlpipalrKARAALTGP--APGTVPWETLVDAAIGGDGSDvS 212
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 171 MADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMApKHWL-CINEDDLVWATAAPGWQKW------VWSPFLsvlg 243
Cdd:PRK05605 213 HPRPTPDDVALILYTSGTTGKPKGAQLTHRNLFANAAQG-KAWVpGLGDGPERVLAALPMFHAYgltlclTLAVSI---- 287
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 244 sGATAFVFNgRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVR 323
Cdd:PRK05605 288 -GGELVLLP-APDIDLILDAMKKHPPTWLPGVPPLYEKIAEAAEERGVDLSGVRNAFSGAMALPVSTVELWEKLTGGLLV 365
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 324 DGYGQTE-STLLIGFLKDTPQRIGSMGKGIPGSSVTVVD--DEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQT 400
Cdd:PRK05605 366 EGYGLTEtSPIIVGNPMSDDRRPGYVGVPFPDTEVRIVDpeDPDETMPDGEEGELLV--RGPQVFKGYWNRPEETAKSFL 443
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 401 GKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGD 480
Cdd:PRK05605 444 DGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALD 523
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|
gi 612673656 481 DTLVKElqqFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEK 530
Cdd:PRK05605 524 PEGLRA---YCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVREELLEK 570
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
47-525 7.43e-47

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 171.93  E-value: 7.43e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  47 ISVTYQQLIEQSNKVGNVLASH-GLSKGDKVLIMMPrSIATYELYI-AALKLGVAIIPCSEMLRTKDLQYRITHGEINAV 124
Cdd:PRK12492  48 VTLSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMP-NVLQYPIAVfGALRAGLIVVNTNPLYTAREMRHQFKDSGARAL 126
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 125 IALEPFTVEFEKIKEYDALTKFVIAGQKD------GWI---------------SL-------EAEKENASNKLEMADTTR 176
Cdd:PRK12492 127 VYLNMFGKLVQEVLPDTGIEYLIEAKMGDllpaakGWLvntvvdkvkkmvpayHLpqavpfkQALRQGRGLSLKPVPVGL 206
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAVTHCHGWGFAHL--------QMAPKHWLCINEDDLVWATAAPGWQKWVWSP-FLSVLGSGA- 246
Cdd:PRK12492 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMlqvraclsQLGPDGQPLMKEGQEVMIAPLPLYHIYAFTAnCMCMMVSGNh 286
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 247 TAFVFNGRFSPeTYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGY 326
Cdd:PRK12492 287 NVLITNPRDIP-GFIKELGKWRFSALLGLNTLFVALMDHPGFKDLDFSALKLTNSGGTALVKATAERWEQLTGCTIVEGY 365
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 327 GQTE-STLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGK-YY 404
Cdd:PRK12492 366 GLTEtSPVASTNPYGELARLGTVGIPVPGTALKVIDDDGNELPLGERGELCI--KGPQVMKGYWQQPEATAEALDAEgWF 443
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 405 ITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGddtlV 484
Cdd:PRK12492 444 KTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDPGLS----V 519
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 612673656 485 KELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK12492 520 EELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELRD 560
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
47-525 2.14e-46

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 169.43  E-value: 2.14e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  47 ISVTYQQLIEQSNKVGNVLASHGLSkGDKVLIMMPRSIATYELYIAALKLG--VAIIPCSEMLRTkdLQYRITHGEINAV 124
Cdd:cd05909    6 TSLTYRKLLTGAIALARKLAKMTKE-GENVGVMLPPSAGGALANFALALSGkvPVMLNYTAGLRE--LRACIKLAGIKTV 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 125 IALEPFtveFEKIKeydaLTKFVIAGQKDGWISLEAEKENASNK------LEM-------------ADTTRDDMAILSYT 185
Cdd:cd05909   83 LTSKQF---IEKLK----LHHLFDVEYDARIVYLEDLRAKISKAdkckafLAGkfppkwllrifgvAPVQPDDPAVILFT 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 186 SGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDdlVWATAAP-----GWQKWVWSPFLSvlgsGATAFVFNGRFSPETY 260
Cdd:cd05909  156 SGSEGLPKGVVLSHKNLLANVEQITAIFDPNPED--VVFGALPffhsfGLTGCLWLPLLS----GIKVVFHPNPLDYKKI 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 261 LELLQKYQINVLCCTPTEYRMMAKlqNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGF-LK 339
Cdd:cd05909  230 PELIYDKKATILLGTPTFLRGYAR--AAHPEDFSSLRLVVAGAEKLKDTLRQEFQEKFGIRILEGYGTTECSPVISVnTP 307
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 340 DTPQRIGSMGKGIPGSSVTVVDDEGNS-VPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYF 418
Cdd:cd05909  308 QSPNKEGTVGRPLPGMEVKIVSVETHEeVPIGEGGLLLV--RGPNVMLGYLNEPELTSFAFGDGWYDTGDIGKIDGEGFL 385
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 419 WFEGRRDDIIISSGYTIGPFEVEDALTNH-PAVKECAVVAKPHDIRGNIVkafVILQDHTAGDdtlVKELQQFVKNEVAP 497
Cdd:cd05909  386 TITGRLSRFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGRKGEKI---VLLTTTTDTD---PSSLNDILKNAGIS 459
                        490       500
                 ....*....|....*....|....*....
gi 612673656 498 YKY-PREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd05909  460 NLAkPSYIHQVEEIPLLGTGKPDYVTLKA 488
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
51-533 5.24e-45

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 166.03  E-value: 5.24e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  51 YQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQY---------RITHGE- 120
Cdd:PRK12406  14 FDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYiledsgarvLIAHADl 93
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 121 INAVIALEPFTVEF-------EKIKEY--DALTKFVIAGQKD--GWisLEAEKENASNKLEMADTtrddmaiLSYTSGTT 189
Cdd:PRK12406  94 LHGLASALPAGVTVlsvptppEIAAAYriSPALLTPPAGAIDweGW--LAQQEPYDGPPVPQPQS-------MIYTSGTT 164
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 190 GNPKAVThchgwgfahlQMAPKHWLCINEDDLVwATA---APGWQKWVWSP--------FLSVLGSGATAFVFNGRFSPE 258
Cdd:PRK12406 165 GHPKGVR----------RAAPTPEQAAAAEQMR-ALIyglKPGIRALLTGPlyhsapnaYGLRAGRLGGVLVLQPRFDPE 233
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 259 TYLELLQKYQINVLCCTPTeyrMMAKLQNLND-----YDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTL 333
Cdd:PRK12406 234 ELLQLIERHRITHMHMVPT---MFIRLLKLPEevrakYDVSSLRHVIHAAAPCPADVKRAMIEWWGPVIYEYYGSTESGA 310
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 LIGFL-KDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKgYYKEPERTAKAQTGKYYITGDLAHV 412
Cdd:PRK12406 311 VTFATsEDALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGNPDFT-YHNKPEKRAEIDRGGFITSGDVGYL 389
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 413 DDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTLVKELQQFVK 492
Cdd:PRK12406 390 DADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQ---PGATLDEADIRAQLK 466
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 612673656 493 NEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAeieKWQQ 533
Cdd:PRK12406 467 ARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDP---YWAN 504
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
50-455 6.09e-45

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 163.59  E-value: 6.09e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   50 TYQQLIEQSNKVGNVL-ASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALE 128
Cdd:TIGR01733   1 TYRELDERANRLARHLrAAGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  129 PFTVEFekikeyDALTKFVIAgqkdgwISLEAEKENASNKLEMADTTR---DDMAILSYTSGTTGNPKAVTHCHGwGFAH 205
Cdd:TIGR01733  81 ALASRL------AGLVLPVIL------LDPLELAALDDAPAPPPPDAPsgpDDLAYVIYTSGSTGRPKGVVVTHR-SLVN 147
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  206 LQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNG--RFSPETYLELLQKYQINVLCCTPTEYRMMA 283
Cdd:TIGR01733 148 LLAWLARRYGLDPDDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDeeRDDAALLAALIAEHPVTVLNLTPSLLALLA 227
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  284 KLQnlnDYDLTHLHSAVSAGEPLNREVVEQFK-KYFNLTVRDGYGQTESTL-----LIGFLKDTPQRIGSMGKGIPGSSV 357
Cdd:TIGR01733 228 AAL---PPALASLRLVILGGEALTPALVDRWRaRGPGARLINLYGPTETTVwstatLVDPDDAPRESPVPIGRPLANTRL 304
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  358 TVVDDEGNSVPantkgnIAVPLDL----PALFKGYYKEPERTAKA---------QTGKYYITGDLAHVDDDGYFWFEGRR 424
Cdd:TIGR01733 305 YVLDDDLRPVP------VGVVGELyiggPGVARGYLNRPELTAERfvpdpfaggDGARLYRTGDLVRYLPDGNLEFLGRI 378
                         410       420       430
                  ....*....|....*....|....*....|.
gi 612673656  425 DDIIISSGYTIGPFEVEDALTNHPAVKECAV 455
Cdd:TIGR01733 379 DDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
32-527 8.75e-45

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 170.04  E-value: 8.75e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIatyELYIA---ALKLGVAIIPcsemLr 108
Cdd:COG1020   490 PDAVAVVF-----GDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSL---EMVVAllaVLKAGAAYVP----L- 556
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  109 tkDLQY---RITH----GEINAVIALEPFTVEFekikeydaltkfviAGQKDGWISLEAEK-ENASNKLEMADTTRDDMA 180
Cdd:COG1020   557 --DPAYpaeRLAYmledAGARLVLTQSALAARL--------------PELGVPVLALDALAlAAEPATNPPVPVTPDDLA 620
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  181 ILSYTSGTTGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPG-----WQkwVWSPflsvLGSGATAFVFN--G 253
Cdd:COG1020   621 YVIYTSGSTGRPKGVMVEHR-ALVNLLAWMQRRYGLGPGDRVLQFASLSfdasvWE--IFGA----LLSGATLVLAPpeA 693
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  254 RFSPETYLELLQKYQINVLCCTPTEYRMMakLQNLNDyDLTHLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTEST 332
Cdd:COG1020   694 RRDPAALAELLARHRVTVLNLTPSLLRAL--LDAAPE-ALPSLRLVLVGGEALPPELVRRWRARLpGARLVNLYGPTETT 770
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  333 L--LIGFLKDTPQRIGSM--GKGIPGSSVTVVDDEGNSVPantkgnIAVPLDL----PALFKGYYKEPERTA-------- 396
Cdd:COG1020   771 VdsTYYEVTPPDADGGSVpiGRPIANTRVYVLDAHLQPVP------VGVPGELyiggAGLARGYLNRPELTAerfvadpf 844
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  397 KAQTGKYYITGDLAHVDDDGYFWFEGRRDD---IiisSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVIL 473
Cdd:COG1020   845 GFPGARLYRTGDLARWLPDGNLEFLGRADDqvkI---RGFRIELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVP 921
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....
gi 612673656  474 QDhtaGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAE 527
Cdd:COG1020   922 EA---GAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDRLALPAPA 972
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
26-523 3.31e-44

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 163.27  E-value: 3.31e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  26 EKYSHIPDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIatyELYI---AALKLGVAIIP 102
Cdd:cd17655    5 EQAEKTPDHTAVVF-----EDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSL---EMIVgilGILKAGGAYLP 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 103 CSEMLRTKDLQYRITHGEINAVIALEPFtvefekiKEYDALTKFVIAgqkdgwISLEAEKENASNKLEmADTTRDDMAIL 182
Cdd:cd17655   77 IDPDYPEERIQYILEDSGADILLTQSHL-------QPPIAFIGLIDL------LDEDTIYHEESENLE-PVSKSDDLAYV 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 183 SYTSGTTGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLgSGATAFVFNG--RFSPETY 260
Cdd:cd17655  143 IYTSGSTGKPKGVMIEHR-GVVNLVEWANKVIYQGEHLRVALFASISFDASVTEIFASLL-SGNTLYIVRKetVLDGQAL 220
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 261 LELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLhsaVSAGEPLNREVVEQFKKYFNLTVR--DGYGQTESTL----- 333
Cdd:cd17655  221 TQYIRQNRITIIDLTPAHLKLLDAADDSEGLSLKHL---IVGGEALSTELAKKIIELFGTNPTitNAYGPTETTVdasiy 297
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 LIGFLKDTPQRIgSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDlpALFKGYYKEPERTAK-------AQTGKYYIT 406
Cdd:cd17655  298 QYEPETDQQVSV-PIGKPLGNTRIYILDQYGRPQPVGVAGELYIGGE--GVARGYLNRPELTAEkfvddpfVPGERMYRT 374
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 407 GDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAgddtlVKE 486
Cdd:cd17655  375 GDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKELP-----VAQ 449
                        490       500       510
                 ....*....|....*....|....*....|....*..
gi 612673656 487 LQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd17655  450 LREFLARELPDYMIPSYFIKLDEIPLTPNGKVDRKAL 486
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
32-523 3.43e-44

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 163.14  E-value: 3.43e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCsemlrtkD 111
Cdd:cd12117   11 PDAVAVVY-----GDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPL-------D 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQY---RITH----GEINAVIALEPFTVEfekikeydaltkfVIAGQKDGWISLEAEKENASNKLEMADTtrDDMAILSY 184
Cdd:cd12117   79 PELpaeRLAFmladAGAKVLLTDRSLAGR-------------AGGLEVAVVIDEALDAGPAGNPAVPVSP--DDLAYVMY 143
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 185 TSGTTGNPK--AVTH------CHGWGFAHLqmapkhwlciNEDDLVWATAAPGWQKW---VWSPFLsvlgSGATAFVFNG 253
Cdd:cd12117  144 TSGSTGRPKgvAVTHrgvvrlVKNTNYVTL----------GPDDRVLQTSPLAFDAStfeIWGALL----NGARLVLAPK 209
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 254 RF--SPETYLELLQKYQINVLCCTPTEYRMMAklqnlnDYD---LTHLHSAVSAGEPLNREVVEQF-KKYFNLTVRDGYG 327
Cdd:cd12117  210 GTllDPDALGALIAEEGVTVLWLTAALFNQLA------DEDpecFAGLRELLTGGEVVSPPHVRRVlAACPGLRLVNGYG 283
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 328 QTESTLligF-----LKDTPQRIGS--MGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDlpALFKGYYKEPERTAK--- 397
Cdd:cd12117  284 PTENTT---FttshvVTELDEVAGSipIGRPIANTRVYVLDEDGRPVPPGVPGELYVGGD--GLALGYLNRPALTAErfv 358
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 398 ----AQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVIL 473
Cdd:cd12117  359 adpfGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDKRLVAYVVA 438
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|
gi 612673656 474 qDHTAGDDtlvkELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd12117  439 -EGALDAA----ELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
184-520 2.98e-43

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 156.41  E-value: 2.98e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 184 YTSGTTGNPKAVTHCHgwgfahlqmapKHWLC---INEDDLVW----ATAAPGwqKWVWSPFL----SVLGSGATaFVFN 252
Cdd:cd17633    7 FTSGTTGLPKAYYRSE-----------RSWIEsfvCNEDLFNIsgedAILAPG--PLSHSLFLygaiSALYLGGT-FIGQ 72
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 253 GRFSPETYLELLQKYQINVLCCTPTeyrMMAKLQNLNDYDlTHLHSAVSAGEPLNREVVEQFKKYFNLTVR-DGYGQTES 331
Cdd:cd17633   73 RKFNPKSWIRKINQYNATVIYLVPT---MLQALARTLEPE-SKIKSIFSSGQKLFESTKKKLKNIFPKANLiEFYGTSEL 148
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 332 TLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVpantkGNIAVplDLPALFKGYYKEPErtakAQTGKYYITGDLAH 411
Cdd:cd17633  149 SFITYNFNQESRPPNSVGRPFPNVEIEIRNADGGEI-----GKIFV--KSEMVFSGYVRGGF----SNPDGWMSVGDIGY 217
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 412 VDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVilqdhtAGDDTLVKELQQFV 491
Cdd:cd17633  218 VDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALY------SGDKLTYKQLKRFL 291
                        330       340
                 ....*....|....*....|....*....
gi 612673656 492 KNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:cd17633  292 KQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
44-524 4.93e-43

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 160.20  E-value: 4.93e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  44 GEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPC---------SEMLRTKDLQY 114
Cdd:cd17651   16 AEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLdpaypaerlAFMLADAGPVL 95
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 115 RITHGEINAVIALEPftvefekikeydaltkfvIAGQKDGWISLEAEKENASNklemADTTRDDMAILSYTSGTTGNPKA 194
Cdd:cd17651   96 VLTHPALAGELAVEL------------------VAVTLLDQPGAAAGADAEPD----PALDADDLAYVIYTSGSTGRPKG 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 195 VTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFlSVLGSGATAFVFNG--RFSPETYLELLQKYQINVL 272
Cdd:cd17651  154 VVMPHR-SLANLVAWQARASSLGPGARTLQFAGLGFDVSVQEIF-STLCAGATLVLPPEevRTDPPALAAWLDEQRISRV 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 273 CCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLnrEVVEQFKKYFN----LTVRDGYGQTESTLLIGF----LKDTPQR 344
Cdd:cd17651  232 FLPTVALRALAEHGRPLGVRLAALRYLLTGGEQL--VLTEDLREFCAglpgLRLHNHYGPTETHVVTALslpgDPAAWPA 309
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 345 IGSMGKGIPGSSVTVVDDEGNSVPantkgnIAVPLDL----PALFKGYYKEPERTAK-------AQTGKYYITGDLAHVD 413
Cdd:cd17651  310 PPPIGRPIDNTRVYVLDAALRPVP------PGVPGELyiggAGLARGYLNRPELTAErfvpdpfVPGARMYRTGDLARWL 383
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 414 DDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTLVKELQQFVKN 493
Cdd:cd17651  384 PDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRLVAYVVGD---PEAPVDAAELRAALAT 460
                        490       500       510
                 ....*....|....*....|....*....|.
gi 612673656 494 EVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd17651  461 HLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
PLN02246 PLN02246
4-coumarate--CoA ligase
26-524 7.63e-43

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 160.53  E-value: 7.63e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  26 EKYSHIPDKKAILYHNTeGEdiSVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSE 105
Cdd:PLN02246  31 ERLSEFSDRPCLIDGAT-GR--VYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANP 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 106 MLRTKDLQYRITHGEINAVIALEPFTvefEKIKEY---DALTKFVIAGQKDGWISLEAEKENASNKLEMADTTRDDMAIL 182
Cdd:PLN02246 108 FYTPAEIAKQAKASGAKLIITQSCYV---DKLKGLaedDGVTVVTIDDPPEGCLHFSELTQADENELPEVEISPDDVVAL 184
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 183 SYTSGTTGNPKAVTHCHG---WGFAHLQMAPKHWLCINEDDLVWAtAAPGWQKW-VWSPFLSVLGSGAtAFVFNGRFSPE 258
Cdd:PLN02246 185 PYSSGTTGLPKGVMLTHKglvTSVAQQVDGENPNLYFHSDDVILC-VLPMFHIYsLNSVLLCGLRVGA-AILIMPKFEIG 262
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 259 TYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFK-KYFNLTVRDGYGQTES----TL 333
Cdd:PLN02246 263 ALLELIQRHKVTIAPFVPPIVLAIAKSPVVEKYDLSSIRMVLSGAAPLGKELEDAFRaKLPNAVLGQGYGMTEAgpvlAM 342
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 LIGFLKD-TPQRIGSMGKGIPGSSVTVVD-DEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKA-QTGKYYITGDLA 410
Cdd:PLN02246 343 CLAFAKEpFPVKSGSCGTVVRNAELKIVDpETGASLPRNQPGEICI--RGPQIMKGYLNDPEATANTiDKDGWLHTGDIG 420
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 411 HVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVIlqdHTAGDDTLVKELQQF 490
Cdd:PLN02246 421 YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVV---RSNGSEITEDEIKQF 497
                        490       500       510
                 ....*....|....*....|....*....|....
gi 612673656 491 VKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PLN02246 498 VAKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLR 531
PRK07638 PRK07638
acyl-CoA synthetase; Validated
21-533 1.26e-42

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 158.79  E-value: 1.26e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  21 IISEIEKY-SHIPDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGlSKGDKVLIMMPRSIATYELYIAALKLGVA 99
Cdd:PRK07638   3 ITKEYKKHaSLQPNKIAIKE-----NDRVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWT 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 100 IIPCSEMLRTKDLQYRITHGEINAVIALEPFTVEFEkikeyDALTKFVIAGQKDGWISLEAEKENASNKLEmadttrDDM 179
Cdd:PRK07638  77 CVPLDIKWKQDELKERLAISNADMIVTERYKLNDLP-----DEEGRVIEIDEWKRMIEKYLPTYAPIENVQ------NAP 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 180 AILSYTSGTTGNPKAVTHCH-GWgfAHLQMAPKHWLCINEDDLVwatAAPGwqKWVWSPFL----SVLGSGATAFVFNgR 254
Cdd:PRK07638 146 FYMGFTSGSTGKPKAFLRAQqSW--LHSFDCNVHDFHMKREDSV---LIAG--TLVHSLFLygaiSTLYVGQTVHLMR-K 217
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 255 FSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLThlhsAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTESTL 333
Cdd:PRK07638 218 FIPNQVLDKLETENISVMYTVPTMLESLYKENRVIENKMK----IISSGAKWEAEAKEKIKNIFpYAKLYEFYGASELSF 293
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 lIGFL--KDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAH 411
Cdd:PRK07638 294 -VTALvdEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTVYV--KSPQFFMGYIIGGVLARELNADGWMTVRDVGY 370
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 412 VDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHtagddtlVKELQQFV 491
Cdd:PRK07638 371 EDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAIIKGSAT-------KQQLKSFC 443
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 612673656 492 KNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD--AEIEKWQQ 533
Cdd:PRK07638 444 LQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSwiENQEKIYE 487
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
32-520 1.64e-42

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 158.22  E-value: 1.64e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd12116    1 PDATAVRD-----DDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADR 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIAlepftvefekikeyDALTKFVIAGQKDGWISLEAEKENASNKLEmADTTRDDMAILSYTSGTTGN 191
Cdd:cd12116   76 LRYILEDAEPALVLT--------------DDALPDRLPAGLPVLLLALAAAAAAPAAPR-TPVSPDDLAYVIYTSGSTGR 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLgSGATAFVFNG--RFSPETYLELLQKYQI 269
Cdd:cd12116  141 PKGVVVSHR-NLVNFLHSMRERLGLGPGDRLLAVTTYAFDISLLELLLPLL-AGARVVIAPRetQRDPEALARLIEAHSI 218
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 270 NVLCCTPTEYRMMAKLQNLNDYDLThlhsAVSAGEPLNREVVEQfkkyfnLTVRDG-----YGQTESTL--LIGFLKDTP 342
Cdd:cd12116  219 TVMQATPATWRMLLDAGWQGRAGLT----ALCGGEALPPDLAAR------LLSRVGslwnlYGPTETTIwsTAARVTAAA 288
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 343 QRIgSMGKGIPGSSVTVVDDEGNSVPAntkgniAVPLDL----PALFKGYYKEPERTAKA--------QTGKYYITGDLA 410
Cdd:cd12116  289 GPI-PIGRPLANTQVYVLDAALRPVPP------GVPGELyiggDGVAQGYLGRPALTAERfvpdpfagPGSRLYRTGDLV 361
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 411 HVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVkAFVILQDHTAGDDTlvkELQQF 490
Cdd:cd12116  362 RRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVREDGGDRRLV-AYVVLKAGAAPDAA---ALRAH 437
                        490       500       510
                 ....*....|....*....|....*....|
gi 612673656 491 VKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:cd12116  438 LRATLPAYMVPSAFVRLDALPLTANGKLDR 467
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
44-520 4.24e-42

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 156.60  E-value: 4.24e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  44 GEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINA 123
Cdd:cd05907    1 GVWQPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKA 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 124 VIALEPftvefekikeydaltkfviagqkdgwisleaekenasnklemadttrDDMAILSYTSGTTGNPKAVTHCHGwgf 203
Cdd:cd05907   81 LFVEDP-----------------------------------------------DDLATIIYTSGTTGRPKGVMLSHR--- 110
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 204 aHLqmapkHWLCINEDDLVwaTAAPGWQKWVWSPFLSVLGSGATAFVF-------NGRFSPETYLELLQKYQINVLCCTP 276
Cdd:cd05907  111 -NI-----LSNALALAERL--PATEGDRHLSFLPLAHVFERRAGLYVPllagariYFASSAETLLDDLSEVRPTVFLAVP 182
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 277 TEYRMMAKLQNLND--------YDLTHLHS---AVSAGEPLNREVVEQFKKyFNLTVRDGYGQTESTLLIGFLKDTPQRI 345
Cdd:cd05907  183 RVWEKVYAAIKVKAvpglkrklFDLAVGGRlrfAASGGAPLPAELLHFFRA-LGIPVYEGYGLTETSAVVTLNPPGDNRI 261
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 346 GSMGKGIPGSSVTVVDDegnsvpantkGNIAVplDLPALFKGYYKEPERTAKAQTGK-YYITGDLAHVDDDGYFWFEGRR 424
Cdd:cd05907  262 GTVGKPLPGVEVRIADD----------GEILV--RGPNVMLGYYKNPEATAEALDADgWLHTGDLGEIDEDGFLHITGRK 329
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 425 DDIII-SSGYTIGPFEVEDALTNHPAVKECAVVA--KPH-----DIRGNIVKAFVILQDHTAGDDT-------LVKELQQ 489
Cdd:cd05907  330 KDLIItSGGKNISPEPIENALKASPLISQAVVIGdgRPFlvaliVPDPEALEAWAEEHGIAYTDVAelaanpaVRAEIEA 409
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 612673656 490 FVKN---EVAPYKYPREIEFVDDLP------KTNSGKIRR 520
Cdd:cd05907  410 AVEAanaRLSRYEQIKKFLLLPEPFtiengeLTPTLKLKR 449
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
239-520 2.37e-41

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 151.65  E-value: 2.37e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 239 LSVLGSGATAFVFNgRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLhSAVSAGEplNREVVEQFKKYF 318
Cdd:cd17637   60 LATFHAGGANVVME-KFDPAEALELIEEEKVTLMGSFPPILSNLLDAAEKSGVDLSSL-RHVLGLD--APETIQRFEETT 135
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 319 NLTVRDGYGQTESTLLIGFlKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKA 398
Cdd:cd17637  136 GATFWSLYGQTETSGLVTL-SPYRERPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVV--RGPLVFQGYWNLPELTAYT 212
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 399 QTGKYYITGDLAHVDDDGYFWFEGRR--DDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdh 476
Cdd:cd17637  213 FRNGWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLK-- 290
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....
gi 612673656 477 tAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:cd17637  291 -PGATLTADELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
178-520 4.30e-41

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 151.26  E-value: 4.30e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 178 DMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAfVFNGRFSP 257
Cdd:cd17635    2 DPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGGLWWILTCLIHGGLCV-TGGENTTY 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 258 ETYLELLQKYQINVLCCTPTEY-RMMAKLQNLNDYDLTHLHSAVSAGEPLNREVveQFKKYFNLT-VRDGYGQTEST--L 333
Cdd:cd17635   81 KSLFKILTTNAVTTTCLVPTLLsKLVSELKSANATVPSLRLIGYGGSRAIAADV--RFIEATGLTnTAQVYGLSETGtaL 158
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 LIGFLKDTPQrIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVD 413
Cdd:cd17635  159 CLPTDDDSIE-INAVGRPYPGVDVYLAATDGIAGPSASFGTIWI--KSPANMLGYWNNPERTAEVLIDGWVNTGDLGERR 235
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 414 DDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTagDDTLVKELQQFVKN 493
Cdd:cd17635  236 EDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASAEL--DENAIRALKHTIRR 313
                        330       340
                 ....*....|....*....|....*..
gi 612673656 494 EVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:cd17635  314 ELEPYARPSTIVIVTDIPRTQSGKVKR 340
FACL_like_5 cd05924
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
177-517 4.71e-41

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341248 [Multi-domain]  Cd Length: 364  Bit Score: 151.77  E-value: 4.71e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILsYTSGTTGNPKAVTHCH---------GWGFAHLQMAPKHWL---CINEDDLVWATAAP---GWQKWVWspfLSV 241
Cdd:cd05924    4 DDLYIL-YTGGTTGMPKGVMWRQedifrmlmgGADFGTGEFTPSEDAhkaAAAAAGTVMFPAPPlmhGTGSWTA---FGG 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 242 LGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTeyrMMAK-----LQNLNDYDLTHLHSAVSAGEPLNREVVEQFKK 316
Cdd:cd05924   80 LLGGQTVVLPDDRFDPEEVWRTIEKHKVTSMTIVGD---AMARplidaLRDAGPYDLSSLFAISSGGALLSPEVKQGLLE 156
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 317 YF-NLTVRDGYGQTESTLLiGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVP--ANTKGNIA----VPLdlpalfkGYY 389
Cdd:cd05924  157 LVpNITLVDAFGSSETGFT-GSGHSAGSGPETGPFTRANPDTVVLDDDGRVVPpgSGGVGWIArrghIPL-------GYY 228
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 390 KEPERTAK----AQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGN 465
Cdd:cd05924  229 GDEAKTAEtfpeVDGVRYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQ 308
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|..
gi 612673656 466 IVKAfvILQDHTAGDDTLvKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGK 517
Cdd:cd05924  309 EVVA--VVQLREGAGVDL-EELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
177-530 5.35e-41

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 151.48  E-value: 5.35e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAVTHCHG------WGFAHLQMapkhwlcINEDDLVWAtAAPGWQKW-VWSPFLSVLGSGATAF 249
Cdd:cd05944    2 DDVAAYFHTGGTTGTPKLAQHTHSnevynaWMLALNSL-------FDPDDVLLC-GLPLFHVNgSVVTLLTPLASGAHVV 73
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 250 VFN--GRFSPETYLE---LLQKYQINVLCCTPTEYrmMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRD 324
Cdd:cd05944   74 LAGpaGYRNPGLFDNfwkLVERYRITSLSTVPTVY--AALLQVPVNADISSLRFAMSGAAPLPVELRARFEDATGLPVVE 151
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 325 GYGQTESTLLIGF-LKDTPQRIGSMGKGIPGSSVTVV--DDEGNSV---PANTKGNIAVPLdlPALFKGY-YKEPERTAK 397
Cdd:cd05944  152 GYGLTEATCLVAVnPPDGPKRPGSVGLRLPYARVRIKvlDGVGRLLrdcAPDEVGEICVAG--PGVFGGYlYTEGNKNAF 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 398 AQTGkYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHT 477
Cdd:cd05944  230 VADG-WLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGA 308
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....
gi 612673656 478 AGDDTlvkELQQFVKNEVAPY-KYPREIEFVDDLPKTNSGKIRRVELRDAEIEK 530
Cdd:cd05944  309 VVEEE---ELLAWARDHVPERaAVPKHIEVLEELPVTAVGKVFKPALRADAIHR 359
PRK06060 PRK06060
p-hydroxybenzoic acid--AMP ligase FadD22;
49-524 1.53e-40

p-hydroxybenzoic acid--AMP ligase FadD22;


Pssm-ID: 180374 [Multi-domain]  Cd Length: 705  Bit Score: 155.96  E-value: 1.53e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  49 VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALE 128
Cdd:PRK06060  31 VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALAARNTEPALVVTSD 110
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 129 PFTVEFEKIKEYDAltkfviagqkdgwisleAEKENASNKLEMAD---TTRDDMAILSYTSGTTGNPKAVTHCHGWGFAH 205
Cdd:PRK06060 111 ALRDRFQPSRVAEA-----------------AELMSEAARVAPGGyepMGGDALAYATYTSGTTGPPKAAIHRHADPLTF 173
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 206 LQMAPKHWLCINEDDLVWATA----APGWQKWVWSPflsvLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTeyrM 281
Cdd:PRK06060 174 VDAMCRKALRLTPEDTGLCSArmyfAYGLGNSVWFP----LATGGSAVINSAPVTPEAAAILSARFGPSVLYGVPN---F 246
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 282 MAKLQNLNDYD-LTHLHSAVSAGEPLNREVVEQFKKYFN-LTVRDGYGQTEstllIG--FLKDTPQ--RIGSMGKGIPGS 355
Cdd:PRK06060 247 FARVIDSCSPDsFRSLRCVVSAGEALELGLAERLMEFFGgIPILDGIGSTE----VGqtFVSNRVDewRLGTLGRVLPPY 322
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 356 SVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPErtAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTI 435
Cdd:PRK06060 323 EIRVVAPDGTTAGPGVEGDLWV--RGPAIAKGYWNRPD--SPVANEGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNV 398
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 436 GPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS 515
Cdd:PRK06060 399 DPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTPN 478

                 ....*....
gi 612673656 516 GKIRRVELR 524
Cdd:PRK06060 479 GKLVRGALR 487
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
32-456 3.62e-40

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 153.72  E-value: 3.62e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAiLYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSiatYELYI---AALKLGVAIIPCSEMLR 108
Cdd:COG1022   25 PDRVA-LREKEDGIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNR---PEWVIadlAILAAGAVTVPIYPTSS 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 TKDLQYRITHGEINAVIALEPFTVEfeKIKEY-DALT--KFVIA------GQKDGWISLEA-----EKENASNKLE--MA 172
Cdd:COG1022  101 AEEVAYILNDSGAKVLFVEDQEQLD--KLLEVrDELPslRHIVVldprglRDDPRLLSLDEllalgREVADPAELEarRA 178
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 173 DTTRDDMAILSYTSGTTGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDLV-----WAtaapgwqkWV----WSpfLSVLG 243
Cdd:COG1022  179 AVKPDDLATIIYTSGTTGRPKGVMLTHR-NLLSNARALLERLPLGPGDRTlsflpLA--------HVfertVS--YYALA 247
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 244 SGAT-AFVFngrfSPETYLELLQKYQINVLCCTPTEY-----RMMAKLQNL------------------NDYDLT----- 294
Cdd:COG1022  248 AGATvAFAE----SPDTLAEDLREVKPTFMLAVPRVWekvyaGIQAKAEEAgglkrklfrwalavgrryARARLAgksps 323
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 295 ------------------------HLHSAVSAGEPLNREVVEqfkkYFN---LTVRDGYGQTESTLLIGFLKDTPQRIGS 347
Cdd:COG1022  324 lllrlkhaladklvfsklrealggRLRFAVSGGAALGPELAR----FFRalgIPVLEGYGLTETSPVITVNRPGDNRIGT 399
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 348 MGKGIPGSSVTVVDDegnsvpaN---TKGniavpldlPALFKGYYKEPERTAKA-QTGKYYITGDLAHVDDDGYFWFEGR 423
Cdd:COG1022  400 VGPPLPGVEVKIAED-------GeilVRG--------PNVMKGYYKNPEATAEAfDADGWLHTGDIGELDEDGFLRITGR 464
                        490       500       510
                 ....*....|....*....|....*....|....
gi 612673656 424 RDDIII-SSGYTIGPFEVEDALTNHPAVKECAVV 456
Cdd:COG1022  465 KKDLIVtSGGKNVAPQPIENALKASPLIEQAVVV 498
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
32-523 8.24e-40

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 150.10  E-value: 8.24e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAIlyhntEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIatyELYIAAL---KLGVAIIPCsemlr 108
Cdd:cd17652    1 PDAPAV-----VFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSA---ELVVAILavlKAGAAYLPL----- 67
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 tkDLQY---RITH--GEINAVIALepftvefekikeydaltkfviagqkdgwisleaekenasnklemadTTRDDMAILS 183
Cdd:cd17652   68 --DPAYpaeRIAYmlADARPALLL----------------------------------------------TTPDNLAYVI 99
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 184 YTSGTTGNPK--AVTHChgwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSpFLSVLGSGATAFVF--NGRFSPET 259
Cdd:cd17652  100 YTSGSTGRPKgvVVTHR---GLANLAAAQIAAFDVGPGSRVLQFASPSFDASVWE-LLMALLAGATLVLApaEELLPGEP 175
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 260 YLELLQKYQINVLCCTPteyrmmAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQF---KKYFNltvrdGYGQTESTLLIG 336
Cdd:cd17652  176 LADLLREHRITHVTLPP------AALAALPPDDLPDLRTLVVAGEACPAELVDRWapgRRMIN-----AYGPTETTVCAT 244
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 337 FLK-DTPQRIGSMGKGIPGSSVTVVDDEGNSVPantkgnIAVPLDL----PALFKGYYKEPERTAK--------AQTGKY 403
Cdd:cd17652  245 MAGpLPGGGVPPIGRPVPGTRVYVLDARLRPVP------PGVPGELyiagAGLARGYLNRPGLTAErfvadpfgAPGSRM 318
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDdtl 483
Cdd:cd17652  319 YRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAPT--- 395
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 612673656 484 VKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd17652  396 AAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
32-525 2.06e-39

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 148.61  E-value: 2.06e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAIlyhntEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcsemLRTKD 111
Cdd:cd17653   11 PDAVAV-----ESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVP----LDAKL 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHgeinavialepftvefekikeydaltkfVIagqkdgwisleaekENASNKLEMADTTRDDMAILSYTSGTTGN 191
Cdd:cd17653   82 PSARIQA----------------------------IL--------------RTSGATLLLTTDSPDDLAYIIFTSGSTGI 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFlSVLGSGATaFVFngRFSPETYLELLQKyqINV 271
Cdd:cd17653  120 PKGVMVPHR-GVLNYVSQPPARLDVGPGSRVAQVLSIAFDACIGEIF-STLCNGGT-LVL--ADPSDPFAHVART--VDA 192
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 272 LCCTPTeyrmmaKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKkyFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKG 351
Cdd:cd17653  193 LMSTPS------ILSTLSPQDFPNLKTIFLGGEAVPPSLLDRWS--PGRRLYNAYGPTECTISSTMTELLPGQPVTIGKP 264
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 352 IPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKA------QTG-KYYITGDLAHVDDDGYFWFEGRR 424
Cdd:cd17653  265 IPNSTCYILDADLQPVPEGVVGEICI--SGVQVARGYLGNPALTASKfvpdpfWPGsRMYRTGDYGRWTEDGGLEFLGRE 342
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 425 DDIIISSGYTIGPFEVED-ALTNHPAVKECAVVAkphdIRGNIVkAFVilqdhtAGDDTLVKELQQFVKNEVAPYKYPRE 503
Cdd:cd17653  343 DNQVKVRGFRINLEEIEEvVLQSQPEVTQAAAIV----VNGRLV-AFV------TPETVDVDGLRSELAKHLPSYAVPDR 411
                        490       500
                 ....*....|....*....|..
gi 612673656 504 IEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd17653  412 IIALDSFPLTANGKVDRKALRE 433
PLN03102 PLN03102
acyl-activating enzyme; Provisional
32-525 6.65e-39

acyl-activating enzyme; Provisional


Pssm-ID: 215576 [Multi-domain]  Cd Length: 579  Bit Score: 149.78  E-value: 6.65e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEgedisVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PLN03102  28 PNRTSIIYGKTR-----FTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATS 102
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVI---ALEPFTVEFEK---------------IKEYDALTK-FVIAGQKDGWISLEAEKENASNKLEMA 172
Cdd:PLN03102 103 IAAILRHAKPKILFvdrSFEPLAREVLHllssedsnlnlpvifIHEIDFPKRpSSEELDYECLIQRGEPTPSLVARMFRI 182
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 173 DTTRDDMAiLSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATaaPGWQKWVWSPFLSVLGSGATAFVFN 252
Cdd:PLN03102 183 QDEHDPIS-LNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTL--PMFHCNGWTFTWGTAARGGTSVCMR 259
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 253 GRFSPETYlELLQKYQINVLCCTPTEYRMMAKLQNLndyDLTHLHSAV---SAGEPLNREVVEQFKKyFNLTVRDGYGQT 329
Cdd:PLN03102 260 HVTAPEIY-KNIEMHNVTHMCCVPTVFNILLKGNSL---DLSPRSGPVhvlTGGSPPPAALVKKVQR-LGFQVMHAYGLT 334
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 330 ESTLLIGF---------LKDTPQRIGSMGKGIPGSSVTVVD----DEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTA 396
Cdd:PLN03102 335 EATGPVLFcewqdewnrLPENQQMELKARQGVSILGLADVDvknkETQESVPRDGKTMGEIVIKGSSIMKGYLKNPKATS 414
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 397 KAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDh 476
Cdd:PLN03102 415 EAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAFVVLEK- 493
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 612673656 477 taGDDTLVKELQQFVKNEVAPYKY----------PREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PLN03102 494 --GETTKEDRVDKLVTRERDLIEYcrenlphfmcPRKVVFLQELPKNGNGKILKPKLRD 550
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
178-520 1.05e-38

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 144.18  E-value: 1.05e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 178 DMAILSYTSGTTGNPKAVTHCH--------GWGFAHLQMAPKHWLCINeddlvwataapgwqkwvwsPFLSVLG--SGAT 247
Cdd:cd17638    1 DVSDIMFTSGTTGRSKGVMCAHrqtlraaaAWADCADLTEDDRYLIIN-------------------PFFHTFGykAGIV 61
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 248 AFVFNGR-------FSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNL 320
Cdd:cd17638   62 ACLLTGAtvvpvavFDVDAILEAIERERITVLPGPPTLFQSLLDHPGRKKFDLSSLRAAVTGAATVPVELVRRMRSELGF 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 321 -TVRDGYGQTES-TLLIGFLKDTPQRIGSM-GKGIPGSSVTVVDDegnsvpantkGNIAVplDLPALFKGYYKEPERTAK 397
Cdd:cd17638  142 eTVLTAYGLTEAgVATMCRPGDDAETVATTcGRACPGFEVRIADD----------GEVLV--RGYNVMQGYLDDPEATAE 209
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 398 AQTGKYYI-TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDH 476
Cdd:cd17638  210 AIDADGWLhTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPG 289
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 612673656 477 TAGDDtlvKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:cd17638  290 VTLTE---EDVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
32-520 5.14e-38

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 145.88  E-value: 5.14e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPC-------- 103
Cdd:cd17646   12 PDAPAVVD-----EGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLdpgypadr 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 104 -SEMLRTKDLQYRITHGEINAVIA--LEPFTVEFEKIKEYDALtkfviagqkdgwisleaekenasnkLEMADTTRDDMA 180
Cdd:cd17646   87 lAYMLADAGPAVVLTTADLAARLPagGDVALLGDEALAAPPAT-------------------------PPLVPPRPDNLA 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 181 ILSYTSGTTGNPKAVTHCHGwGFAHlQMApkhWLC----INEDDLVWATAAPGWQKWVWSPFLSvLGSGATAFVF--NGR 254
Cdd:cd17646  142 YVIYTSGSTGRPKGVMVTHA-GIVN-RLL---WMQdeypLGPGDRVLQKTPLSFDVSVWELFWP-LVAGARLVVArpGGH 215
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 255 FSPETYLELLQKYQINVLCCTPTeyrMMAK-LQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTL 333
Cdd:cd17646  216 RDPAYLAALIREHGVTTCHFVPS---MLRVfLAEPAAGSCASLRRVFCSGEALPPELAARFLALPGAELHNLYGPTEAAI 292
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 LIGFLK---DTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAK-------AQTGKY 403
Cdd:cd17646  293 DVTHWPvrgPAETPSVPIGRPVPNTRLYVLDDALRPVPVGVPGELY--LGGVQLARGYLGRPALTAErfvpdpfGPGSRM 370
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTl 483
Cdd:cd17646  371 YRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGAAGPDT- 449
                        490       500       510
                 ....*....|....*....|....*....|....*..
gi 612673656 484 vKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:cd17646  450 -AALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDR 485
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
48-530 5.24e-38

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 147.05  E-value: 5.24e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPrSIATYELY-IAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIA 126
Cdd:PLN02330  55 AVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLP-NVAEYGIVaLGIMAAGGVFSGANPTALESEIKKQAEAAGAKLIVT 133
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 127 LEpftVEFEKIKEYD----ALTKFVIAGQKDgWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWG 202
Cdd:PLN02330 134 ND---TNYGKVKGLGlpviVLGEEKIEGAVN-WKELLEAADRAGDTSDNEEILQTDLCALPFSSGTTGISKGVMLTHRNL 209
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 203 FAHLQMApkhwLCINEDDLVWATAAPGWqkwvwSPFLSVLGSGATAF---------VFNGRFSPETYLELLQKYQINVLC 273
Cdd:PLN02330 210 VANLCSS----LFSVGPEMIGQVVTLGL-----IPFFHIYGITGICCatlrnkgkvVVMSRFELRTFLNALITQEVSFAP 280
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 274 CTPTEYRMMAKLQNLNDYDLTHL--HSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTESTLLIGFLKDTPQRIG---- 346
Cdd:PLN02330 281 IVPPIILNLVKNPIVEEFDLSKLklQAIMTAAAPLAPELLTAFEAKFpGVQVQEAYGLTEHSCITLTHGDPEKGHGiakk 360
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 347 -SMGKGIPGSSVTVVD-DEGNSVPANTKGNIAVPLDlpALFKGYYKEPERTAKAQTGKYYI-TGDLAHVDDDGYFWFEGR 423
Cdd:PLN02330 361 nSVGFILPNLEVKFIDpDTGRSLPKNTPGELCVRSQ--CVMQGYYNNKEETDRTIDEDGWLhTGDIGYIDDDGDIFIVDR 438
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 424 RDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDtlvKELQQFVKNEVAPYKYPRE 503
Cdd:PLN02330 439 IKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACVVINPKAKESE---EDILNFVAANVAHYKKVRV 515
                        490       500
                 ....*....|....*....|....*..
gi 612673656 504 IEFVDDLPKTNSGKIRRVELRDAEIEK 530
Cdd:PLN02330 516 VQFVDSIPKSLSGKIMRRLLKEKMLSI 542
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
25-525 5.11e-37

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 144.12  E-value: 5.11e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  25 IEKYSHIPDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCS 104
Cdd:PRK06018  16 IDHAARIHGNREVVTRSVEGPIVRTTYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVN 95
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 105 EMLRTKDLQYRITHGEINAVIALEPFTVEFEKIKEY-DALTKFVIAGQKD--------GWISLEAEKENASNKLEMADTT 175
Cdd:PRK06018  96 PRLFPEQIAWIINHAEDRVVITDLTFVPILEKIADKlPSVERYVVLTDAAhmpqttlkNAVAYEEWIAEADGDFAWKTFD 175
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 176 RDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMApkhwlciNEDDLVWATAA-------PGWQKWVWSPFLSVLGSGATA 248
Cdd:PRK06018 176 ENTAAGMCYTSGTTGDPKGVLYSHRSNVLHALMA-------NNGDALGTSAAdtmlpvvPLFHANSWGIAFSAPSMGTKL 248
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 249 FVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYfNLTVRDGYGQ 328
Cdd:PRK06018 249 VMPGAKLDGASVYELLDTEKVTFTAGVPTVWLMLLQYMEKEGLKLPHLKMVVCGGSAMPRSMIKAFEDM-GVEVRHAWGM 327
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 329 TE-------STLLIGFLKDTP-QRIGSMGK-GIP--GSSVTVVDDEGNSVP--ANTKGNIAVplDLPALFKGYYKEPERT 395
Cdd:PRK06018 328 TEmsplgtlAALKPPFSKLPGdARLDVLQKqGYPpfGVEMKITDDAGKELPwdGKTFGRLKV--RGPAVAAAYYRVDGEI 405
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 396 AKAQtgKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNivKAFVILQd 475
Cdd:PRK06018 406 LDDD--GFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDE--RPLLIVQ- 480
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|
gi 612673656 476 HTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK06018 481 LKPGETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALRE 530
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
32-523 6.17e-37

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 142.07  E-value: 6.17e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAIlyhntEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd12115   13 PDAIAL-----VCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPER 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIalepftvefekikeydaltkfviagqkdgwisleaekenasnklemadTTRDDMAILSYTSGTTGN 191
Cdd:cd12115   88 LRFILEDAQARLVL------------------------------------------------TDPDDLAYVIYTSGSTGR 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGWGFAHLQMAPKHWlciNEDDL--VWATAAPGWQKWVWSPFLSvLGSGATAFVFNGRFSPETYLELLQKYQI 269
Cdd:cd12115  120 PKGVAIEHRNAAAFLQWAAAAF---SAEELagVLASTSICFDLSVFELFGP-LATGGKVVLADNVLALPDLPAAAEVTLI 195
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 270 NVLcctPTeyrMMAKLQNLNDydlthLHSAVS----AGEPLNREVVE------QFKKYFNLtvrdgYGQTESTLLIGFLK 339
Cdd:cd12115  196 NTV---PS---AAAELLRHDA-----LPASVRvvnlAGEPLPRDLVQrlyarlQVERVVNL-----YGPSEDTTYSTVAP 259
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 340 DTPQRIG--SMGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAK-------AQTGKYYITGDLA 410
Cdd:cd12115  260 VPPGASGevSIGRPLANTQAYVLDRALQPVPLGVPGELY--IGGAGVARGYLGRPGLTAErflpdpfGPGARLYRTGDLV 337
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 411 HVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGddtLVKELQQF 490
Cdd:cd12115  338 RWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAG---LVEDLRRH 414
                        490       500       510
                 ....*....|....*....|....*....|...
gi 612673656 491 VKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd12115  415 LGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
32-518 8.52e-36

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 139.07  E-value: 8.52e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDK-VLIMMPRSIATYELYIAALKLGVAIIPCsemlrtk 110
Cdd:cd17648    1 PDRVAVVY-----GDKRLTYRELNERANRLAHYLLSVAEIRPDDlVGLVLDKSELMIIAILAVWKAGAAYVPI------- 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 111 DLQYrithgeinavialePftvefekikeyDALTKFVIagqkdgwisleaekENASNKLEMADTTrdDMAILSYTSGTTG 190
Cdd:cd17648   69 DPSY--------------P-----------DERIQFIL--------------EDTGARVVITNST--DLAYAIYTSGTTG 107
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 191 NPKAVTHCHGwGFAHL--QMAPKHWLCINEDDLVWATAApgwqkWVWSPF-----LSVLGsGATAFVFN--GRFSPETYL 261
Cdd:cd17648  108 KPKGVLVEHG-SVVNLrtSLSERYFGRDNGDEAVLFFSN-----YVFDFFveqmtLALLN-GQKLVVPPdeMRFDPDRFY 180
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 262 ELLQKYQINVLCCTPTEyrmmaklqnLNDYDL---THLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTL--LIG 336
Cdd:cd17648  181 AYINREKVTYLSGTPSV---------LQQYDLarlPHLKRVDAAGEEFTAPVFEKLRSRFAGLIINAYGPTETTVtnHKR 251
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 337 FLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPAlfKGYYKEPERTA------------KAQTGKY- 403
Cdd:cd17648  252 FFPGDQRFDKSLGRPVRNTKCYVLNDAMKRVPVGAVGELYLGGDGVA--RGYLNRPELTAerflpnpfqteqERARGRNa 329
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 --YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAK--PHDIRGNIVKAFV--ILQDHT 477
Cdd:cd17648  330 rlYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVAKedASQAQSRIQKYLVgyYLPEPG 409
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 612673656 478 AGDDTlvkELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI 518
Cdd:cd17648  410 HVPES---DLLSFLRAKLPRYMVPARLVRLEGIPVTINGKL 447
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
178-525 1.02e-35

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 135.92  E-value: 1.02e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 178 DMAILSYTSGTTGNPKAVTHCHGwgfAHL-----------QMAPKHWLCINEddLV-WATAAPGWqKWVWSPFLSVLGSG 245
Cdd:cd17630    1 RLATVILTSGSTGTPKAVVHTAA---NLLasaaglhsrlgFGGGDSWLLSLP--LYhVGGLAILV-RSLLAGAELVLLER 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 246 ATAFVFNGRFSPETYLELlqkyqinvlccTPTEYRMMakLQNLNDYD-LTHLHSAVSAGEPLNREVVEQFKKYfNLTVRD 324
Cdd:cd17630   75 NQALAEDLAPPGVTHVSL-----------VPTQLQRL--LDSGQGPAaLKSLRAVLLGGAPIPPELLERAADR-GIPLYT 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 325 GYGQTE--STLLIGFLKDTPQriGSMGKGIPGSSVTVVDDegnsvpantkGNIAVplDLPALFKGYYKEPERTAKAQTGK 402
Cdd:cd17630  141 TYGMTEtaSQVATKRPDGFGR--GGVGVLLPGRELRIVED----------GEIWV--GGASLAMGYLRGQLVPEFNEDGW 206
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 403 YYiTGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVilqdhTAGDDT 482
Cdd:cd17630  207 FT-TKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVI-----VGRGPA 280
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|...
gi 612673656 483 LVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:cd17630  281 DPAELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRA 323
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
20-520 1.48e-35

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 139.26  E-value: 1.48e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  20 NIISEIEKYSHI-PDKKAILYHNTEgedisVTYQQLIEQSNKVGNVLASHGLSKGDKVLI---MMPRSIATYelyIAALK 95
Cdd:PRK04813   3 DIIETIEEFAQTqPDFPAYDYLGEK-----LTYGQLKEDSDALAAFIDSLKLPDKSPIIVfghMSPEMLATF---LGAVK 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  96 LGVAIIPC---SEMLRTKDLqyrITHGEINAVIALEPFTVEFEKIKEYDAltkfviAGQKDGWISLEAEKENASNKLema 172
Cdd:PRK04813  75 AGHAYIPVdvsSPAERIEMI---IEVAKPSLIIATEELPLEILGIPVITL------DELKDIFATGNPYDFDHAVKG--- 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 173 dttrDDMAILSYTSGTTGNPKAVThchgwgfahlqmapkhwlcINEDDLV----WAT---AAPGWQKWV-WSPF---LSV 241
Cdd:PRK04813 143 ----DDNYYIIFTSGTTGKPKGVQ-------------------ISHDNLVsftnWMLedfALPEGPQFLnQAPYsfdLSV 199
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 242 ------LGSGATAFVF------NgrfsPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNRE 309
Cdd:PRK04813 200 mdlyptLASGGTLVALpkdmtaN----FKQLFETLPQLPINVWVSTPSFADMCLLDPSFNEEHLPNLTHFLFCGEELPHK 275
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 310 VVEQFKKYF-NLTVRDGYGQTESTLLIGFLKDTPQRIGSM-----GKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPA 383
Cdd:PRK04813 276 TAKKLLERFpSATIYNTYGPTEATVAVTSIEITDEMLDQYkrlpiGYAKPDSPLLIIDEEGTKLPDGEQGEIVI--SGPS 353
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 384 LFKGYYKEPERTAKA--QTGKY--YITGDLAHVdDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA-- 457
Cdd:PRK04813 354 VSKGYLNNPEKTAEAffTFDGQpaYHTGDAGYL-EDGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVESAVVVPyn 432
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 612673656 458 KPHDIRgNIVkAFVILQDHTAGDD-TLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:PRK04813 433 KDHKVQ-YLI-AYVVPKEEDFEREfELTKAIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDR 494
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
42-520 1.60e-35

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 138.34  E-value: 1.60e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  42 TEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEI 121
Cdd:cd05914    1 LYYGGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEA 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 122 NAVialepftvefekikeydaltkFViagqkdgwisleaekenasnklemadTTRDDMAILSYTSGTTGNPKAV--THCH 199
Cdd:cd05914   81 KAI---------------------FV--------------------------SDEDDVALINYTSGTTGNSKGVmlTYRN 113
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 200 GWGFAHlqmAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATaFVFNGRFSPETyLELLQKYQI--NVLCCTPT 277
Cdd:cd05914  114 IVSNVD---GVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAH-VVFLDKIPSAK-IIALAFAQVtpTLGVPVPL 188
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 278 E-------------------YRMMAKLQNLNDYDLT----------HLHSAVSAGEPLNREVVEQFKKyFNLTVRDGYGQ 328
Cdd:cd05914  189 ViekifkmdiipkltlkkfkFKLAKKINNRKIRKLAfkkvheafggNIKEFVIGGAKINPDVEEFLRT-IGFPYTIGYGM 267
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 329 TESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEgnsvPANTKGNIAVplDLPALFKGYYKEPERTAKAQT--GKYYiT 406
Cdd:cd05914  268 TETAPIISYSPPNRIRLGSAGKVIDGVEVRIDSPD----PATGEGEIIV--RGPNVMKGYYKNPEATAEAFDkdGWFH-T 340
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 407 GDLAHVDDDGYFWFEGRRDDIII-SSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIV----KAFVILQDHTAGDD 481
Cdd:cd05914  341 GDLGKIDAEGYLYIRGRKKEMIVlSSGKNIYPEEIEAKINNMPFVLESLVVVQEKKLVALAYidpdFLDVKALKQRNIID 420
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 612673656 482 TLVKELQQFVKNEVAPYKYPREIEFV-DDLPKTNSGKIRR 520
Cdd:cd05914  421 AIKWEVRDKVNQKVPNYKKISKVKIVkEEFEKTPKGKIKR 460
PRK07867 PRK07867
acyl-CoA synthetase; Validated
172-532 1.79e-35

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 139.43  E-value: 1.79e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 172 ADTTRDDMAILSYTSGTTGNPKAVTHCHG-WGFAHLQMAPKHWLCinEDDLVWAT----------AApgwqkwvWSPFLS 240
Cdd:PRK07867 147 RVADPDDLFMLIFTSGTSGDPKAVRCTHRkVASAGVMLAQRFGLG--PDDVCYVSmplfhsnavmAG-------WAVALA 217
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 241 vlgSGAtAFVFNGRFSPETYLELLQKYQINVL--CCTPTEYrMMAKLQNLNDYDLTHlhSAVSAGEPLNREVvEQFKKYF 318
Cdd:PRK07867 218 ---AGA-SIALRRKFSASGFLPDVRRYGATYAnyVGKPLSY-VLATPERPDDADNPL--RIVYGNEGAPGDI-ARFARRF 289
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 319 NLTVRDGYGQTESTLLIGFLKDTPQriGSMGKGIPGssVTVVD-DEGNSVP-----ANTKGNIA------VPLDLPALFK 386
Cdd:PRK07867 290 GCVVVDGFGSTEGGVAITRTPDTPP--GALGPLPPG--VAIVDpDTGTECPpaedaDGRLLNADeaigelVNTAGPGGFE 365
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 387 GYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNI 466
Cdd:PRK07867 366 GYYNDPEADAERMRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQ 445
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 612673656 467 VKAFVILQDHTAGDDTlvkELQQFV--KNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRdaeIEKWQ 532
Cdd:PRK07867 446 VMAALVLAPGAKFDPD---AFAEFLaaQPDLGPKQWPSYVRVCAELPRTATFKVLKRQLS---AEGVD 507
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
32-524 1.86e-35

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 137.88  E-value: 1.86e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd17649    1 PDAVALVF-----GDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAER 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPftvefekikeydaltkfviagqkdgwisleaekenasnklemadttrDDMAILSYTSGTTGN 191
Cdd:cd17649   76 LRYMLEDSGAGLLLTHHP-----------------------------------------------RQLAYVIYTSGSTGT 108
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGWGFAHLQ-------MAPK----HWLCINEDdlvwaTAAPGWqkwvwspfLSVLGSGATAFVFNGR--FSPE 258
Cdd:cd17649  109 PKGVAVSHGPLAAHCQataerygLTPGdrelQFASFNFD-----GAHEQL--------LPPLICGACVVLRPDElwASAD 175
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 259 TYLELLQKYQINVLCCTPTEYRMMAK-LQNLNDYDLTHLHSAVSAGEPLNREVVEQFKK----YFNltvrdGYGQTEST- 332
Cdd:cd17649  176 ELAEMVRELGVTVLDLPPAYLQQLAEeADRTGDGRPPSLRLYIFGGEALSPELLRRWLKapvrLFN-----AYGPTEATv 250
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 333 --LLIGFLKDTPQRIGSM--GKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAK--------AQT 400
Cdd:cd17649  251 tpLVWKCEAGAARAGASMpiGRPLGGRSAYILDADLNPVPVGVTGELY--IGGEGLARGYLGRPELTAErfvpdpfgAPG 328
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 401 GKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVkAFVILQDHTAGD 480
Cdd:cd17649  329 SRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDGAGGKQLV-AYVVLRAAAAQP 407
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 612673656 481 DtLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd17649  408 E-LRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
32-523 3.34e-35

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 137.79  E-value: 3.34e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHntegeDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd12114    1 PDATAVICG-----DGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAAR 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPftvefekikeYDALTKFVIAGQKDGWISLEAEKENASnklemADTTRDDMAILSYTSGTTGN 191
Cdd:cd12114   76 REAILADAGARLVLTDGP----------DAQLDVAVFDVLILDLDALAAPAPPPP-----VDVAPDDLAYVIFTSGSTGT 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAV--THchgwgfahlQMAPKHWLCINE------DDLVWATAAPGWQKWVWSPFlSVLGSGATaFVF---NGRFSPETY 260
Cdd:cd12114  141 PKGVmiSH---------RAALNTILDINRrfavgpDDRVLALSSLSFDLSVYDIF-GALSAGAT-LVLpdeARRRDPAHW 209
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 261 LELLQKYQINVLCCTPteyrmmAKLQNLndydLTHLHSAVSAGEPLnREVV-----------EQFKKYF-NLTVRDGYGQ 328
Cdd:cd12114  210 AELIERHGVTLWNSVP------ALLEML----LDVLEAAQALLPSL-RLVLlsgdwipldlpARLRALApDARLISLGGA 278
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 329 TE----STLL-IGflkDTPQRIGSM--GKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALfkGYYKEPERTAK---- 397
Cdd:cd12114  279 TEasiwSIYHpID---EVPPDWRSIpyGRPLANQRYRVLDPRGRDCPDWVPGELWIGGRGVAL--GYLGDPELTAArfvt 353
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 398 -AQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVkAFVILQDH 476
Cdd:cd12114  354 hPDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVVLGDPGGKRLA-AFVVPDND 432
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 612673656 477 TAGDDTLvkELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd12114  433 GTPIAPD--ALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
239-516 1.71e-34

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 132.81  E-value: 1.71e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 239 LSVLGSGATAFVFNG------RFSPETYLELLQKYQinvlcCT------PTeyrmMAKLQNLND---YDLTHLHSAVSAG 303
Cdd:cd17636   53 IGTLMFTLATFHAGGtnvfvrRVDAEEVLELIEAER-----CThafllpPT----IDQIVELNAdglYDLSSLRSSPAAP 123
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 304 E--PLNREVVEQFKKYFNltvrdGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDL 381
Cdd:cd17636  124 EwnDMATVDTSPWGRKPG-----GYGQTEVMGLATFAALGGGAIGGAGRPSPLVQVRILDEDGREVPDGEVGEIVA--RG 196
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 382 PALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHD 461
Cdd:cd17636  197 PTVMAGYWNRPEVNARRTRGGWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDP 276
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 612673656 462 IRGNIVKAFVILQ-DHTAGDDtlvkELQQFVKNEVAPYKYPREIEFVDDLPKTNSG 516
Cdd:cd17636  277 RWAQSVKAIVVLKpGASVTEA----ELIEHCRARIASYKKPKSVEFADALPRTAGG 328
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
32-523 2.32e-34

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 135.29  E-value: 2.32e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd17656    2 PDAVAVVF-----ENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEER 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKenasnklemadttrDDMAILSYTSGTTGN 191
Cdd:cd17656   77 RIYIMLDSGVRVVLTQRHLKSKLSFNKSTILLEDPSISQEDTSNIDYINNS--------------DDLLYIIYTSGTTGK 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKwVWSPFLSVLGSGATAFVFN--GRFSPETYLELLQKYQI 269
Cdd:cd17656  143 PKGVQLEHK-NMVNLLHFEREKTNINFSDKVLQFATCSFDV-CYQEIFSTLLSGGTLYIIReeTKRDVEQLFDLVKRHNI 220
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 270 NVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPL--NREVVEQFKKYfNLTVRDGYGQTESTLLIGFL---KDTPQR 344
Cdd:cd17656  221 EVVFLPVAFLKFIFSEREFINRFPTCVKHIITAGEQLviTNEFKEMLHEH-NVHLHNHYGPSETHVVTTYTinpEAEIPE 299
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 345 IGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAKA-------QTGKYYITGDLAHVDDDGY 417
Cdd:cd17656  300 LPPIGKPISNTWIYILDQEQQLQPQGIVGELYI--SGASVARGYLNRQELTAEKffpdpfdPNERMYRTGDLARYLPDGN 377
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 418 FWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKA-FVILQDHTagddtlVKELQQFVKNEVA 496
Cdd:cd17656  378 IEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAyFVMEQELN------ISQLREYLAKQLP 451
                        490       500
                 ....*....|....*....|....*..
gi 612673656 497 PYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd17656  452 EYMIPSFFVPLDQLPLTPNGKVDRKAL 478
PRK12467 PRK12467
peptide synthase; Provisional
32-523 3.17e-34

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 138.76  E-value: 3.17e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   32 PDKKAILYhnteGEDIsVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSE------ 105
Cdd:PRK12467  526 PERPALVF----GEQV-LSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPeypqdr 600
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  106 ---MLRTKDLQYRITHGEINAVIALEpftvefekikeyDALTKFVIAgQKDGWISLEAEkENASNKLEmadttRDDMAIL 182
Cdd:PRK12467  601 layMLDDSGVRLLLTQSHLLAQLPVP------------AGLRSLCLD-EPADLLCGYSG-HNPEVALD-----PDNLAYV 661
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  183 SYTSGTTGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSvLGSGATAFV--FNGRFSPETY 260
Cdd:PRK12467  662 IYTSGSTGQPKGVAISHG-ALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTELFGA-LASGATLHLlpPDCARDAEAF 739
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  261 LELLQKYQINVLCCTPTEYRMMakLQNLNDYDLTHLHSAVSAGEPLNREVVEQ-FKKYFNLTVRDGYGQTESTL--LIGF 337
Cdd:PRK12467  740 AALMADQGVTVLKIVPSHLQAL--LQASRVALPRPQRALVCGGEALQVDLLARvRALGPGARLINHYGPTETTVgvSTYE 817
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  338 LKDTPQRIGS--MGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDlpALFKGYYKEPERTAK--------AQTGKYYITG 407
Cdd:PRK12467  818 LSDEERDFGNvpIGQPLANLGLYILDHYLNPVPVGVVGELYIGGA--GLARGYHRRPALTAErfvpdpfgADGGRLYRTG 895
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  408 DLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDiRGNIVKAFVIL------QDHTAGDD 481
Cdd:PRK12467  896 DLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPGD-AGLQLVAYLVPaavadgAEHQATRD 974
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|...
gi 612673656  482 TLVKELQQfvkneVAP-YKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:PRK12467  975 ELKAQLRQ-----VLPdYMVPAHLLLLDSLPLTPNGKLDRKAL 1012
PLN02574 PLN02574
4-coumarate--CoA ligase-like
20-525 3.64e-34

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 136.12  E-value: 3.64e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  20 NIISEIEKYSHIPDKKAILYHNTeGEDISVTYQQLIEQSNKVG--NVLashGLSKGDKVLIMMPRSIATYELYIAALKLG 97
Cdd:PLN02574  41 DAVSFIFSHHNHNGDTALIDSST-GFSISYSELQPLVKSMAAGlyHVM---GVRQGDVVLLLLPNSVYFPVIFLAVLSLG 116
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  98 VAIIPCSEMLRTKDLQYRIThgEINAVIALepftVEFEKIKEYDALTKFVIaGQKDGWISLEAEKENASNKLEMADTT-- 175
Cdd:PLN02574 117 GIVTTMNPSSSLGEIKKRVV--DCSVGLAF----TSPENVEKLSPLGVPVI-GVPENYDFDSKRIEFPKFYELIKEDFdf 189
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 176 -------RDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINED---DLVWATAAPGWQKWVWSPFLSVLGSG 245
Cdd:PLN02574 190 vpkpvikQDDVAAIMYSSGTTGASKGVVLTHRNLIAMVELFVRFEASQYEYpgsDNVYLAALPMFHIYGLSLFVVGLLSL 269
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 246 ATAFVFNGRFSPETYLELLQKYQINVLCCTPTeyRMMAklqnlndydLTHLHSAVSAG------------EPLNREVVEQ 313
Cdd:PLN02574 270 GSTIVVMRRFDASDMVKVIDRFKVTHFPVVPP--ILMA---------LTKKAKGVCGEvlkslkqvscgaAPLSGKFIQD 338
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 314 FKKYF-NLTVRDGYGQTESTLL--IGFLKDTPQRIGSMGKGIPGSSVTVVD-DEGNSVPANTKGNIAvpLDLPALFKGYY 389
Cdd:PLN02574 339 FVQTLpHVDFIQGYGMTESTAVgtRGFNTEKLSKYSSVGLLAPNMQAKVVDwSTGCLLPPGNCGELW--IQGPGVMKGYL 416
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 390 KEPERTA-KAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVK 468
Cdd:PLN02574 417 NNPKATQsTIDKDGWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPV 496
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 612673656 469 AFVILQDHTAGDDTLVKElqqFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PLN02574 497 AFVVRRQGSTLSQEAVIN---YVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKR 550
PRK06164 PRK06164
acyl-CoA synthetase; Validated
42-536 2.09e-33

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 133.72  E-value: 2.09e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  42 TEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEI 121
Cdd:PRK06164  29 LIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRGRA 108
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 122 nAVIALEPftvEFEKIK--------EYDALTK----FVIAGQKD--------GWISLEAEKENASNKLEMADTTRDDMAI 181
Cdd:PRK06164 109 -RWLVVWP---GFKGIDfaailaavPPDALPPlraiAVVDDAADatpapapgARVQLFALPDPAPPAAAGERAADPDAGA 184
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 182 LSYT-SGTTGNPKAVTH------CHGWGFAHLqmapkhwLCINEDDLVWAtAAPGWQKWVWSPFLSVLGSGATAF---VF 251
Cdd:PRK06164 185 LLFTtSGTTSGPKLVLHrqatllRHARAIARA-------YGYDPGAVLLA-ALPFCGVFGFSTLLGALAGGAPLVcepVF 256
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 252 NGrfsPETyLELLQKYQINVLCCTPTEYRMMAKLQNlNDYDLTHLHS-AVSAGEPLNREVVEQFKKYfNLTVRDGYGQTE 330
Cdd:PRK06164 257 DA---ART-ARALRRHRVTHTFGNDEMLRRILDTAG-ERADFPSARLfGFASFAPALGELAALARAR-GVPLTGLYGSSE 330
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 -STLLIGFLKDTPQRIGSMGKGIPGSS---VTVVDDEGNSV-PANTKGNIAVplDLPALFKGYYKEPERTAKAQTGK-YY 404
Cdd:PRK06164 331 vQALVALQPATDPVSVRIEGGGRPASPearVRARDPQDGALlPDGESGEIEI--RAPSLMRGYLDNPDATARALTDDgYF 408
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 405 ITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDirGNIVK-AFVILQDHTAGDDtl 483
Cdd:PRK06164 409 RTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGATRD--GKTVPvAFVIPTDGASPDE-- 484
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 612673656 484 vKELQQFVKNEVAPYKYPREIEFVDDLPKT---NSGKIRRVELRDAEIEKWQQQKD 536
Cdd:PRK06164 485 -AGLMAACREALAGFKVPARVQVVEAFPVTesaNGAKIQKHRLREMAQARLAAERA 539
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
32-526 2.39e-33

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 133.56  E-value: 2.39e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEGEDIsVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMmprsIATYELYIAAL---KLGvAIIPCsemLR 108
Cdd:cd05906   24 PTKGITYIDADGSEEF-QSYQDLLEDARRLAAGLRQLGLRPGDSVILQ----FDDNEDFIPAFwacVLA-GFVPA---PL 94
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 TKDLQYRITHGEIN------------AVIALEPFTVEFEKIKEYDALTKFVIagqkdgwISLEAEKENASNKlEMADTTR 176
Cdd:cd05906   95 TVPPTYDEPNARLRklrhiwqllgspVVLTDAELVAEFAGLETLSGLPGIRV-------LSIEELLDTAADH-DLPQSRP 166
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAVTHCHGwgfahlQM----APKHWLC-INEDDLVWataapgwqkwVWSPFLSVLGSG------ 245
Cdd:cd05906  167 DDLALLMLTSGSTGFPKAVPLTHR------NIlarsAGKIQHNgLTPQDVFL----------NWVPLDHVGGLVelhlra 230
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 246 ----------ATAFVFNgrfSPETYLELLQKYQINVlccTPTEYRMMAKLQNL------NDYDLTHLHSAVSAGEPLNRE 309
Cdd:cd05906  231 vylgcqqvhvPTEEILA---DPLRWLDLIDRYRVTI---TWAPNFAFALLNDLleeiedGTWDLSSLRYLVNAGEAVVAK 304
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 310 VVEQF----KKY-FNLTV-RDGYGQTESTLLIGFLKDTPQ-------RIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIA 376
Cdd:cd05906  305 TIRRLlrllEPYgLPPDAiRPAFGMTETCSGVIYSRSFPTydhsqalEFVSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQ 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 377 VplDLPALFKGYYKEPERTAKAQT-GKYYITGDLAHVdDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAV 455
Cdd:cd05906  385 V--RGPVVTKGYYNNPEANAEAFTeDGWFRTGDLGFL-DNGNLTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPSFT 461
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 612673656 456 VAKPHDIRGNIVKAFVIL----QDHTAGDDTLVKELQQFVKNEV---APYKYPREIEfvdDLPKTNSGKIRRVELRDA 526
Cdd:cd05906  462 AAFAVRDPGAETEELAIFfvpeYDLQDALSETLRAIRSVVSREVgvsPAYLIPLPKE---EIPKTSLGKIQRSKLKAA 536
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
172-525 3.32e-33

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 132.84  E-value: 3.32e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 172 ADTTRDDMAILSYTSGTTGNPKAVTHCHGW-GFAHLQMAPKHWLciNEDDLVW---------ATAApgwqkwVWSPflsV 241
Cdd:PRK13388 145 REVDAMDPFMLIFTSGTTGAPKAVRCSHGRlAFAGRALTERFGL--TRDDVCYvsmplfhsnAVMA------GWAP---A 213
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 242 LGSGATaFVFNGRFSPETYLELLQKYQINVL--CCTPTEYrMMAKLQNLNDYDLThlhSAVSAGEPLNREVVEQFKKYFN 319
Cdd:PRK13388 214 VASGAA-VALPAKFSASGFLDDVRRYGATYFnyVGKPLAY-ILATPERPDDADNP---LRVAFGNEASPRDIAEFSRRFG 288
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 320 LTVRDGYGQTESTLLIGFLKDTPQriGSMGKGIPGssVTVVDDEGNSVPA---------------------NTKGniavp 378
Cdd:PRK13388 289 CQVEDGYGSSEGAVIVVREPGTPP--GSIGRGAPG--VAIYNPETLTECAvarfdahgallnadeaigelvNTAG----- 359
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 379 ldlPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAK 458
Cdd:PRK13388 360 ---AGFFEGYYNNPEATAERMRHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAV 436
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 612673656 459 PHDIRGNIVKAFVILQDhtaGDDTLVKELQQFVKN--EVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK13388 437 PDERVGDQVMAALVLRD---GATFDPDAFAAFLAAqpDLGTKAWPRYVRIAADLPSTATNKVLKRELIA 502
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
32-526 3.67e-33

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 131.90  E-value: 3.67e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAIlyhntEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd05918   13 PDAPAV-----CAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLDPSHPLQR 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRIThgEINAVIALepftvefekikeydaltkfviagqkdgwisleaekenASNKlemadttrDDMAILSYTSGTTGN 191
Cdd:cd05918   88 LQEILQ--DTGAKVVL-------------------------------------TSSP--------SDAAYVIFTSGSTGK 120
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAV------------THCHGWGFAH----LQMAPKHW-LCINEddlvwataapgwqkwvwspFLSVLGSGATAFVfngr 254
Cdd:cd05918  121 PKGVviehralstsalAHGRALGLTSesrvLQFASYTFdVSILE-------------------IFTTLAAGGCLCI---- 177
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 255 fSPETYL-----ELLQKYQINVLCCTPTeyrmMAKLqnLNDYDLTHLHSAVSAGEPLNREVVEQFKKyfNLTVRDGYGQT 329
Cdd:cd05918  178 -PSEEDRlndlaGFINRLRVTWAFLTPS----VARL--LDPEDVPSLRTLVLGGEALTQSDVDTWAD--RVRLINAYGPA 248
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 330 ESTLL-IGFLKDTPQRIGSMGKGIPGSS-VTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAKA--------- 398
Cdd:cd05918  249 ECTIAaTVSPVVPSTDPRNIGRPLGATCwVVDPDNHDRLVPIGAVGELL--IEGPILARGYLNDPEKTAAAfiedpawlk 326
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 399 -----QTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNH-PAVKECAV-VAKPHD--IRGNIVkA 469
Cdd:cd05918  327 qegsgRGRRLYRTGDLVRYNPDGSLEYVGRKDTQVKIRGQRVELGEIEHHLRQSlPGAKEVVVeVVKPKDgsSSPQLV-A 405
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 612673656 470 FVILQDHTAGDD--------------TLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDA 526
Cdd:cd05918  406 FVVLDGSSSGSGdgdslflepsdefrALVAELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRALREL 476
PRK12316 PRK12316
peptide synthase; Provisional
32-520 2.13e-32

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 133.16  E-value: 2.13e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK12316 4565 PDAVAVVF-----DEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRER 4639
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  112 LQYRIthgEINAVIALEPFTVEFEKIKEYDALTKFVIaGQKDGWISLEAEkeNASNKLEmadttRDDMAILSYTSGTTGN 191
Cdd:PRK12316 4640 LAYMM---EDSGAALLLTQSHLLQRLPIPDGLASLAL-DRDEDWEGFPAH--DPAVRLH-----PDNLAYVIYTSGSTGR 4708
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  192 PKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATA----APGWQkWVWspflsVLGSGATAFVFN-GRFSPETYLELLQK 266
Cdd:PRK12316 4709 PKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSfsfdGSHEG-LYH-----PLINGASVVIRDdSLWDPERLYAEIHE 4782
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  267 YQINVLCCTPTEYRMMAKlQNLNDYDLTHLHSAVSAGEPLNREVVEQ-FKKYFNLTVRDGYGQTEST---LLIGFLKDTP 342
Cdd:PRK12316 4783 HRVTVLVFPPVYLQQLAE-HAERDGEPPSLRVYCFGGEAVAQASYDLaWRALKPVYLFNGYGPTETTvtvLLWKARDGDA 4861
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  343 QRIGSM--GKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPAlfKGYYKEPERTAK--------AQTGKYYITGDLAHV 412
Cdd:PRK12316 4862 CGAAYMpiGTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVA--RGYLERPALTAErfvpdpfgAPGGRLYRTGDLARY 4939
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  413 DDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVkAFVILQDHTAGDDT-----LVKEL 487
Cdd:PRK12316 4940 RADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAVGKQLV-GYVVPQDPALADADeaqaeLRDEL 5018
                         490       500       510
                  ....*....|....*....|....*....|...
gi 612673656  488 QQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:PRK12316 5019 KAALRERLPEYMVPAHLVFLARMPLTPNGKLDR 5051
PRK09274 PRK09274
peptide synthase; Provisional
29-456 8.74e-32

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 128.86  E-value: 8.74e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  29 SHIPDKKAILYHNTEGEDISVTYQ-----QLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPC 103
Cdd:PRK09274  17 QERPDQLAVAVPGGRGADGKLAYDelsfaELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKAGAVPVLV 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 104 SEMLRTKDLQYRITHGEINAVIALEPFTVE---FEKIKeyDALTKFVIAGQKDGW--ISLE-AEKENASNKLEMADTTRD 177
Cdd:PRK09274  97 DPGMGIKNLKQCLAEAQPDAFIGIPKAHLArrlFGWGK--PSVRRLVTVGGRLLWggTTLAtLLRDGAAAPFPMADLAPD 174
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 178 DMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHW-LCINEDDLvwATAapgwqkwvwsPFLSVLGS--GATAFVFNGR 254
Cdd:PRK09274 175 DMAAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYgIEPGEIDL--PTF----------PLFALFGPalGMTSVIPDMD 242
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 255 FS------PETYLELLQKYQINVLCCTPteyrmmAKLQNLNDYDLTHLHS------AVSAGEPLNREVVEQFKKYFNLTV 322
Cdd:PRK09274 243 PTrpatvdPAKLFAAIERYGVTNLFGSP------ALLERLGRYGEANGIKlpslrrVISAGAPVPIAVIERFRAMLPPDA 316
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 323 R--DGYGQTESTLL--IG---FLKDTPQRIGSM-----GKGIPGSSVTVVD---------DEGNSVPANTKGNIAVplDL 381
Cdd:PRK09274 317 EilTPYGATEALPIssIEsreILFATRAATDNGagicvGRPVDGVEVRIIAisdapipewDDALRLATGEIGEIVV--AG 394
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 382 PALFKGYYKEPERTAKA-----QTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVV 456
Cdd:PRK09274 395 PMVTRSYYNRPEATRLAkipdgQGDVWHRMGDLGYLDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALV 474
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
32-526 1.16e-31

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 128.51  E-value: 1.16e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILY-HNTEGEDISVTYQQLIEQSNKVGNVLASHGLsKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTK 110
Cdd:cd05931    7 PDRPAYTFlDDEGGREETLTYAELDRRARAIAARLQAVGK-PGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPPTPGR 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 111 DlQYRITH----GEINAVIALEPFTVEFEKIKEYDAltkfviAGQKDGWISLEAEKENASNKLEMADTTRDDMAILSYTS 186
Cdd:cd05931   86 H-AERLAAiladAGPRVVLTTAAALAAVRAFAASRP------AAGTPRLLVVDLLPDTSAADWPPPSPDPDDIAYLQYTS 158
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 187 GTTGNPKAVTHCHGWGFAHLQMAPKHWLCinEDDLVWATaapgwqkwvWSPF----------LSVLGSGATAfVFngrFS 256
Cdd:cd05931  159 GSTGTPKGVVVTHRNLLANVRQIRRAYGL--DPGDVVVS---------WLPLyhdmgligglLTPLYSGGPS-VL---MS 223
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 257 PETY-------LELLQKYQINV---------LCCTpteyrmMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF-- 318
Cdd:cd05931  224 PAAFlrrplrwLRLISRYRATIsaapnfaydLCVR------RVRDEDLEGLDLSSWRVALNGAEPVRPATLRRFAEAFap 297
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 319 -NL---TVRDGYGQTESTLLI----------------------GFLKDTP----QRIGSMGKGIPGSSVTVVDDEGNS-V 367
Cdd:cd05931  298 fGFrpeAFRPSYGLAEATLFVsggppgtgpvvlrvdrdalagrAVAVAADdpaaRELVSCGRPLPDQEVRIVDPETGReL 377
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 368 PANTKGNIAVplDLPALFKGYYKEPERTAKAQT-------GKYYITGDLAHVDDdGYFWFEGRRDDIIISSGYTIGPFEV 440
Cdd:cd05931  378 PDGEVGEIWV--RGPSVASGYWGRPEATAETFGalaatdeGGWLRTGDLGFLHD-GELYITGRLKDLIIVRGRNHYPQDI 454
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 441 EDALTN-HPAVKE--CAVVAKPHDIRGnivkAFVILQDHTAG-----DDTLVKELQQFVKNE--VAPykypREIEFV--D 508
Cdd:cd05931  455 EATAEEaHPALRPgcVAAFSVPDDGEE----RLVVVAEVERGadpadLAAIAAAIRAAVAREhgVAP----ADVVLVrpG 526
                        570
                 ....*....|....*...
gi 612673656 509 DLPKTNSGKIRRVELRDA 526
Cdd:cd05931  527 SIPRTSSGKIQRRACRAA 544
PRK08633 PRK08633
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
172-518 2.51e-31

2-acyl-glycerophospho-ethanolamine acyltransferase; Validated


Pssm-ID: 236315 [Multi-domain]  Cd Length: 1146  Bit Score: 129.27  E-value: 2.51e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  172 ADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQmAPKHWLCINEDDLVWATAapgwqkwvwsPFLSVLGSGATAFV- 250
Cdd:PRK08633  777 PTFKPDDTATIIFSSGSEGEPKGVMLSHHNILSNIE-QISDVFNLRNDDVILSSL----------PFFHSFGLTVTLWLp 845
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  251 ----FNGRFSP-----ETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLT 321
Cdd:PRK08633  846 llegIKVVYHPdptdaLGIAKLVAKHRATILLGTPTFLRLYLRNKKLHPLMFASLRLVVAGAEKLKPEVADAFEEKFGIR 925
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  322 VRDGYGQTESTLLI----------GFLKDTPQRIGSMGKGIPGSSVTVVD-DEGNSVPANTKGNIAVplDLPALFKGYYK 390
Cdd:PRK08633  926 ILEGYGATETSPVAsvnlpdvlaaDFKRQTGSKEGSVGMPLPGVAVRIVDpETFEELPPGEDGLILI--GGPQVMKGYLG 1003
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  391 EPERTAKA----QTGKYYITGDLAHVDDDGYFWFEGR--RDDII----ISSGytigpfEVEDALtnHPAVK----ECAVV 456
Cdd:PRK08633 1004 DPEKTAEVikdiDGIGWYVTGDKGHLDEDGFLTITDRysRFAKIggemVPLG------AVEEEL--AKALGgeevVFAVT 1075
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 612673656  457 AKPHDIRGnivKAFVILQDHTAGDdtlVKELQQFVKN-EVAPYKYPREIEFVDDLPKTNSGKI 518
Cdd:PRK08633 1076 AVPDEKKG---EKLVVLHTCGAED---VEELKRAIKEsGLPNLWKPSRYFKVEALPLLGSGKL 1132
PRK12316 PRK12316
peptide synthase; Provisional
44-533 2.83e-31

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 129.69  E-value: 2.83e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   44 GEdISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPC---------SEMLRTKDLQY 114
Cdd:PRK12316  533 GE-ETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLdpeypaerlAYMLEDSGVQL 611
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  115 RITHgeiNAVIALEPFTVEFEKIkEYDALTKFViAGQKDGWISLEAEKENasnklemadttrddMAILSYTSGTTGNPKA 194
Cdd:PRK12316  612 LLSQ---SHLGRKLPLAAGVQVL-DLDRPAAWL-EGYSEENPGTELNPEN--------------LAYVIYTSGSTGKPKG 672
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  195 VTHCHGWGFAHLQMApKHWLCINEDDLVWATAAPGWQKWVWSPFLSvLGSGATAFVF--NGRFSPETYLELLQKYQINVL 272
Cdd:PRK12316  673 AGNRHRALSNRLCWM-QQAYGLGVGDTVLQKTPFSFDVSVWEFFWP-LMSGARLVVAapGDHRDPAKLVELINREGVDTL 750
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  273 CCTPTeyRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQ-FKKYFNLTVRDGYGQTESTllIGFLKDTPQRIG----S 347
Cdd:PRK12316  751 HFVPS--MLQAFLQDEDVASCTSLRRIVCSGEALPADAQEQvFAKLPQAGLYNLYGPTEAA--IDVTHWTCVEEGgdsvP 826
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  348 MGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAK-------AQTGKYYITGDLAHVDDDGYFWF 420
Cdd:PRK12316  827 IGRPIANLACYILDANLEPVPVGVLGELY--LAGRGLARGYHGRPGLTAErfvpspfVAGERMYRTGDLARYRADGVIEY 904
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  421 EGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAkphdIRGNIVKAFVILQDhTAGDdtLVKELQQFVKNEVAPYKY 500
Cdd:PRK12316  905 AGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLA----VDGKQLVGYVVLES-EGGD--WREALKAHLAASLPEYMV 977
                         490       500       510
                  ....*....|....*....|....*....|...
gi 612673656  501 PREIEFVDDLPKTNSGKIRRVELRDAEIEKWQQ 533
Cdd:PRK12316  978 PAQWLALERLPLTPNGKLDRKALPAPEASVAQQ 1010
PRK12467 PRK12467
peptide synthase; Provisional
45-529 3.58e-31

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 129.13  E-value: 3.58e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   45 EDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPC---------SEMLRTKDLQYR 115
Cdd:PRK12467 3117 GDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLdpeyprerlAYMIEDSGVKLL 3196
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  116 ITHGEInavialepftveFEKIKEYDALTKFVIAGqkdgwISLEAEKENASNKLEMADttrdDMAILSYTSGTTGNPKAV 195
Cdd:PRK12467 3197 LTQAHL------------LEQLPAPAGDTALTLDR-----LDLNGYSENNPSTRVMGE----NLAYVIYTSGSTGKPKGV 3255
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  196 THCHGWGFAHLQ-MAPKHWLCINEDDLVWATAApgWQKWVWSpFLSVLGSGATAFVFNGR-FSPETYLELLQKYQINVLC 273
Cdd:PRK12467 3256 GVRHGALANHLCwIAEAYELDANDRVLLFMSFS--FDGAQER-FLWTLICGGCLVVRDNDlWDPEELWQAIHAHRISIAC 3332
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  274 CTPteyrmmAKLQNLNDY----DLTHLHSAVSAGEPLNREVVEQ-FKKYFNLTVRDGYGQTESTLLIGFLK-DTPQRIGS 347
Cdd:PRK12467 3333 FPP------AYLQQFAEDaggaDCASLDIYVFGGEAVPPAAFEQvKRKLKPRGLTNGYGPTEAVVTVTLWKcGGDAVCEA 3406
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  348 ----MGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLpaLFKGYYKEPERTAK--------AQTGKYYITGDLAHVDDD 415
Cdd:PRK12467 3407 pyapIGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGVG--LARGYHQRPSLTAErfvadpfsGSGGRLYRTGDLARYRAD 3484
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  416 GYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDiRGNIVKAFVILQDHTAGddtLVKELQQFVKNEV 495
Cdd:PRK12467 3485 GVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLARDGA-GGKQLVAYVVPADPQGD---WRETLRDHLAASL 3560
                         490       500       510
                  ....*....|....*....|....*....|....
gi 612673656  496 APYKYPREIEFVDDLPKTNSGKIRRVELRDAEIE 529
Cdd:PRK12467 3561 PDYMVPAQLLVLAAMPLGPNGKVDRKALPDPDAK 3594
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
32-523 6.46e-31

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 125.88  E-value: 6.46e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILyhnteGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK13383  49 PGRTAII-----DDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDA 123
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFTVEfekikeydaltkfvIAGQKDGWISLE---AEKENASNKLEMADTTRddmaILSYTSGT 188
Cdd:PRK13383 124 LAAALRAHHISTVVADNEFAER--------------IAGADDAVAVIDpatAGAEESGGRPAVAAPGR----IVLLTSGT 185
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 189 TGNPKAVTHchgwgfahlqmAPKHWLCINeddlVWATAAP------GWQKWVWSPFLSVLGSGATAF-------VFNGR- 254
Cdd:PRK13383 186 TGKPKGVPR-----------APQLRSAVG----VWVTILDrtrlrtGSRISVAMPMFHGLGLGMLMLtialggtVLTHRh 250
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 255 FSPETYLELLQKYQINVLCCTPTeyrMMAKLQNLNDY-----DLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQT 329
Cdd:PRK13383 251 FDAEAALAQASLHRADAFTAVPV---VLARILELPPRvrarnPLPQLRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGST 327
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 330 EstLLIGFL------KDTPQrigSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPAlfKGYykePERTAKAQTGKY 403
Cdd:PRK13383 328 E--VGIGALatpadlRDAPE---TVGKPVAGCPVRILDRNNRPVGPRVTGRIFVGGELAG--TRY---TDGGGKAVVDGM 397
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhtAGDDTL 483
Cdd:PRK13383 398 TSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLH---PGSGVD 474
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 612673656 484 VKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:PRK13383 475 AAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
PRK12316 PRK12316
peptide synthase; Provisional
46-534 5.35e-30

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 125.84  E-value: 5.35e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   46 DISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVI 125
Cdd:PRK12316 3080 EQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLL 3159
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  126 ALEPFTVEfekikeydaltkfviAGQKDGWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAH 205
Cdd:PRK12316 3160 SQSHLRLP---------------LAQGVQVLDLDRGDENYAEANPAIRTMPENLAYVIYTSGSTGKPKGVGIRHSALSNH 3224
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  206 LQmAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSvLGSGATAFVFNGRF--SPETYLELLQKYQINVLCCTPTEYRMMa 283
Cdd:PRK12316 3225 LC-WMQQAYGLGVGDRVLQFTTFSFDVFVEELFWP-LMSGARVVLAGPEDwrDPALLVELINSEGVDVLHAYPSMLQAF- 3301
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  284 kLQNLNDYDLTHLHSAVSAGEPLNREVVEQFkkYFNLTVRDGYGQTESTLLIGFLKDTPQRIGS--MGKGIPGSSVTVVD 361
Cdd:PRK12316 3302 -LEEEDAHRCTSLKRIVCGGEALPADLQQQV--FAGLPLYNLYGPTEATITVTHWQCVEEGKDAvpIGRPIANRACYILD 3378
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  362 DEGNSVPANTKGNIAVPLDLPAlfKGYYKEPERTAK-------AQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYT 434
Cdd:PRK12316 3379 GSLEPVPVGALGELYLGGEGLA--RGYHNRPGLTAErfvpdpfVPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFR 3456
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  435 IGPFEVEDALTNHPAVKECAVVAkphdIRGNIVKAFVILQDHTAgddTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTN 514
Cdd:PRK12316 3457 IELGEIEARLLEHPWVREAVVLA----VDGRQLVAYVVPEDEAG---DLREALKAHLKASLPEYMVPAHLLFLERMPLTP 3529
                         490       500
                  ....*....|....*....|
gi 612673656  515 SGKIRRVELRDAEIEKWQQQ 534
Cdd:PRK12316 3530 NGKLDRKALPRPDAALLQQD 3549
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
32-523 1.65e-29

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 121.39  E-value: 1.65e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd17644   14 PDAVAVVF-----EDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPLDPNYPQER 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIalepftvefekikeydaltkfviagqkdgwisleaekenasnklemadTTRDDMAILSYTSGTTGN 191
Cdd:cd17644   89 LTYILEDAQISVLL------------------------------------------------TQPENLAYVIYTSGSTGK 120
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGWGFAHLQMAPKHWLCINEDDlVWATAAPGWQKWVWSPFLSVLgSGATAFVFNG--RFSPETYLELLQKYQI 269
Cdd:cd17644  121 PKGVMIEHQSLVNLSHGLIKEYGITSSDR-VLQFASIAFDVAAEEIYVTLL-SGATLVLRPEemRSSLEDFVQYIQQWQL 198
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 270 NVLCCTPTEYRMMAKLQNLNDYDL-THLHSAVSAGEPLNREVVEQFKKYF--NLTVRDGYGQTESTL---LIGFLKDTPQ 343
Cdd:cd17644  199 TVLSLPPAYWHLLVLELLLSTIDLpSSLRLVIVGGEAVQPELVRQWQKNVgnFIQLINVYGPTEATIaatVCRLTQLTER 278
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 344 RIGS--MGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAK---------AQTGKYYITGDLAHV 412
Cdd:cd17644  279 NITSvpIGRPIANTQVYILDENLQPVPVGVPGELH--IGGVGLARGYLNRPELTAEkfishpfnsSESERLYKTGDLARY 356
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 413 DDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDdtlVKELQQFVK 492
Cdd:cd17644  357 LPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYIVPHYEESPS---TVELRQFLK 433
                        490       500       510
                 ....*....|....*....|....*....|.
gi 612673656 493 NEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd17644  434 AKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
ttLC_FACS_like cd05915
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
33-524 3.89e-29

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.


Pssm-ID: 213283 [Multi-domain]  Cd Length: 509  Bit Score: 120.61  E-value: 3.89e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  33 DKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDL 112
Cdd:cd05915    9 GRKEVVSRLHTGEVHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEI 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 113 qyrithgeinaVIALEPftvefEKIKEYDALTKFV-IAGQKDGWISLEAEKENASNKLEMAD---------------TTR 176
Cdd:cd05915   89 -----------AYILNH-----AEDKVLLFDPNLLpLVEAIRGELKTVQHFVVMDEKAPEGYlayeealgeeadpvrVPE 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNGRFS 256
Cdd:cd05915  153 RAACGMAYTTGTTGLPKGVVYSHRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGPRLD 232
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 257 PETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPlNREVVEQFKKYFNLTVRDGYGQTE------ 330
Cdd:cd05915  233 PASLVELFDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSA-APRSLIARFERMGVEVRQGYGLTEtspvvv 311
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 STLLIGFLKDTPQRIGSMGKGIPG-----SSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERT-AKAQTGKYY 404
Cdd:cd05915  312 QNFVKSHLESLSEEEKLTLKAKTGlpiplVRLRVADEEGRPVPKDGKALGEVQLKGPWITGGYYGNEEATrSALTPDGFF 391
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 405 ITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDtlv 484
Cdd:cd05915  392 RTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPRGEKPTPE--- 468
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 612673656 485 kELQQFVKNEVAPYKY-PREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd05915  469 -ELNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALR 508
AMP-binding_C pfam13193
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ...
439-517 8.71e-29

AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.


Pssm-ID: 463804 [Multi-domain]  Cd Length: 76  Bit Score: 108.79  E-value: 8.71e-29
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 612673656  439 EVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtaGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGK 517
Cdd:pfam13193   1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKP---GVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
PTZ00237 PTZ00237
acetyl-CoA synthetase; Provisional
184-520 1.10e-28

acetyl-CoA synthetase; Provisional


Pssm-ID: 240325 [Multi-domain]  Cd Length: 647  Bit Score: 120.62  E-value: 1.10e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 184 YTSGTTGNPKAVTHCHGwgfAHLQMAPKHWLCINEDDL---VWATAAPGWQKWvWSPFLSVLGSGATAFVFNGRFSP--- 257
Cdd:PTZ00237 261 YTSGTTGNSKAVVRSNG---PHLVGLKYYWRSIIEKDIptvVFSHSSIGWVSF-HGFLYGSLSLGNTFVMFEGGIIKnkh 336
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 258 -ETYL-ELLQKYQINVLCCTPTEYRMMAKL----QNLND-YDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTE 330
Cdd:PTZ00237 337 iEDDLwNTIEKHKVTHTLTLPKTIRYLIKTdpeaTIIRSkYDLSNLKEIWCGGEVIEESIPEYIENKLKIKSSRGYGQTE 416
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 S--TLLIGFLKDTPQRIGSmgkGIPGSSV--TVVDDEGNSVPANTKGNIAVPLDLPALFKG-YYKEPERTAKAQTG--KY 403
Cdd:PTZ00237 417 IgiTYLYCYGHINIPYNAT---GVPSIFIkpSILSEDGKELNVNEIGEVAFKLPMPPSFATtFYKNDEKFKQLFSKfpGY 493
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 404 YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVIL----QDHTAG 479
Cdd:PTZ00237 494 YNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDCYNVPIGLLVLkqdqSNQSID 573
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|.
gi 612673656 480 DDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:PTZ00237 574 LNKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPR 614
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
32-520 1.45e-28

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 119.22  E-value: 1.45e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK05852  30 PEAPALVV---TADRIAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPALPIAE 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRIthGEINAVIALEPFTVEFEKIKE---YDALTKFVIAGQKDGWISLEAEKENASNKLEMADTT---RDDMAILSYT 185
Cdd:PRK05852 107 QRVRS--QAAGARVVLIDADGPHDRAEPttrWWPLTVNVGGDSGPSGGTLSVHLDAATEPTPATSTPeglRPDDAMIMFT 184
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 186 SGTTGNPKAVThchgWGFAHLQmAPKHWLC----INEDDlvwATAA--PGWQ-KWVWSPFLSVLGSGATAFV-FNGRFSP 257
Cdd:PRK05852 185 GGTTGLPKMVP----WTHANIA-SSVRAIItgyrLSPRD---ATVAvmPLYHgHGLIAALLATLASGGAVLLpARGRFSA 256
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 258 ETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTH--LHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEST--- 332
Cdd:PRK05852 257 HTFWDDIKAVGATWYTAVPTIHQILLERAATEPSGRKPaaLRFIRSCSAPLTAETAQALQTEFAAPVVCAFGMTEAThqv 336
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 333 -----LLIGFLKDTPQRIGSMGKGIpGSSVTVVDDEGNSVPANTKGNiaVPLDLPALFKGYYKEPERTAKAQTGKYYITG 407
Cdd:PRK05852 337 tttqiEGIGQTENPVVSTGLVGRST-GAQIRIVGSDGLPLPAGAVGE--VWLRGTTVVRGYLGDPTITAANFTDGWLRTG 413
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 408 DLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAgddTLVKEL 487
Cdd:PRK05852 414 DLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPRESAP---PTAEEL 490
                        490       500       510
                 ....*....|....*....|....*....|...
gi 612673656 488 QQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:PRK05852 491 VQFCRERLAAFEIPASFQEASGLPHTAKGSLDR 523
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
26-523 5.23e-28

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 116.50  E-value: 5.23e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  26 EKYSHIPDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcse 105
Cdd:cd17645    6 EQVERTPDHVAVVD-----RGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVP--- 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 106 mlrtkdlqyrithgeinaviaLEPftvefekikEYdaltkfviAGQKDGWISLEAEKenasnKLEMADTtrDDMAILSYT 185
Cdd:cd17645   78 ---------------------IDP---------DY--------PGERIAYMLADSSA-----KILLTNP--DDLAYVIYT 112
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 186 SGTTGNPKAVTHCH-------GWGFAHLQMAPkhwlciNEDDLVWATAapGWQKWVWSPFLSVLgSGATAFVFNgrfsPE 258
Cdd:cd17645  113 SGSTGLPKGVMIEHhnlvnlcEWHRPYFGVTP------ADKSLVYASF--SFDASAWEIFPHLT-AGAALHVVP----SE 179
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 259 TYLEL--LQKY------QINVLCcTPTEYRMMAkLQNlndydlTHLHSAVSAGEPLNREVVEQFKKYfnltvrDGYGQTE 330
Cdd:cd17645  180 RRLDLdaLNDYfnqegiTISFLP-TGAAEQFMQ-LDN------QSLRVLLTGGDKLKKIERKGYKLV------NNYGPTE 245
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 STLLI-GFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDlpALFKGYYKEPERTAKAQTG-------K 402
Cdd:cd17645  246 NTVVAtSFEIDKPYANIPIGKPIDNTRVYILDEALQLQPIGVAGELCIAGE--GLARGYLNRPELTAEKFIVhpfvpgeR 323
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 403 YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVilqdhTAGDDT 482
Cdd:cd17645  324 MYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAYV-----TAPEEI 398
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 612673656 483 LVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd17645  399 PHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
49-522 4.41e-27

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 115.09  E-value: 4.41e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  49 VTYQQLIEQSNKVGNVLASHGLSKGDKVLIM--MPRSIATyeLYIAALKLGVAIIpcseML----RTKDLQY-------R 115
Cdd:PRK07768  30 HTWGEVHERARRIAGGLAAAGVGPGDAVAVLagAPVEIAP--TAQGLWMRGASLT----MLhqptPRTDLAVwaedtlrV 103
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 116 ITHGEINAVIALEPFTVEFEKIKEydaltkfviagqkDGWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAV 195
Cdd:PRK07768 104 IGMIGAKAVVVGEPFLAAAPVLEE-------------KGIRVLTVADLLAADPIDPVETGEDDLALMQLTSGSTGSPKAV 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 196 --THCHGWGFAHLQMAPKHwlcINEDDLVWATaapgwqkwvWSP---------FLSV-LGSGATA-FVFNGRF--SPETY 260
Cdd:PRK07768 171 qiTHGNLYANAEAMFVAAE---FDVETDVMVS---------WLPlfhdmgmvgFLTVpMYFGAELvKVTPMDFlrDPLLW 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 261 LELLQKYQINVLCCTPTEY----RMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQF---KKYFNL---TVRDGYGQTE 330
Cdd:PRK07768 239 AELISKYRGTMTAAPNFAYallaRRLRRQAKPGAFDLSSLRFALNGAEPIDPADVEDLldaGARFGLrpeAILPAYGMAE 318
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 STLLIGF--------------------------LKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDlpAL 384
Cdd:PRK07768 319 ATLAVSFspcgaglvvdevdadllaalrravpaTKGNTRRLATLGPPLPGLEVRVVDEDGQVLPPRGVGVIELRGE--SV 396
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 385 FKGyYKEPERTAKAQ-TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDiR 463
Cdd:PRK07768 397 TPG-YLTMDGFIPAQdADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAAARVEGVRPGNAVAVRLD-A 474
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 612673656 464 GNIVKAF-VILQDHTAGDDTLVKELQQFVKNEV--APYKYPREIEFVD--DLPKTNSGKIRRVE 522
Cdd:PRK07768 475 GHSREGFaVAVESNAFEDPAEVRRIRHQVAHEVvaEVGVRPRNVVVLGpgSIPKTPSGKLRRAN 538
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
32-524 6.52e-27

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 114.97  E-value: 6.52e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVaiipCSEMLRT-- 109
Cdd:PRK08279  51 PDRPALLF-----EDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGA----VVALLNTqq 121
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 110 --KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFV-IAGQK-----DGWISLEAEKENAS--NKLEMADTTRDDM 179
Cdd:PRK08279 122 rgAVLAHSLNLVDAKHLIVGEELVEAFEEARADLARPPRLwVAGGDtlddpEGYEDLAAAAAGAPttNPASRSGVTAKDT 201
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 180 AILSYTSGTTGNPKA--VTHcHGW-----GFAHLqmapkhwLCINEDDLVW----------ATAAPGwqkwvwspflSVL 242
Cdd:PRK08279 202 AFYIYTSGTTGLPKAavMSH-MRWlkamgGFGGL-------LRLTPDDVLYcclplyhntgGTVAWS----------SVL 263
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 243 GSGATaFVFNGRFSPETYLELLQKYQINV------LCctpteyRMMAKlQNLNDYDLTH-LHSAVSAGepLNREVVEQFK 315
Cdd:PRK08279 264 AAGAT-LALRRKFSASRFWDDVRRYRATAfqyigeLC------RYLLN-QPPKPTDRDHrLRLMIGNG--LRPDIWDEFQ 333
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 316 KYFNLT-VRDGYGQTESTL-LIGFLkdtpQRIGSMGKgIPGSSVT---VVD-DEGNSVPA-NTKGN-IAVPLDLPAL--- 384
Cdd:PRK08279 334 QRFGIPrILEFYAASEGNVgFINVF----NFDGTVGR-VPLWLAHpyaIVKyDVDTGEPVrDADGRcIKVKPGEVGLlig 408
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 385 -------FKGyYKEPERTAK-------AQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAV 450
Cdd:PRK08279 409 ritdrgpFDG-YTDPEASEKkilrdvfKKGDAWFNTGDLMRDDGFGHAQFVDRLGDTFRWKGENVATTEVENALSGFPGV 487
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 612673656 451 KECAV--VAKP-HDirGNIVKAFVILQDHTAGDdtlVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK08279 488 EEAVVygVEVPgTD--GRAGMAAIVLADGAEFD---LAALAAHLYERLPAYAVPLFVRLVPELETTGTFKYRKVDLR 559
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
32-523 9.20e-27

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 112.95  E-value: 9.20e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEgedisVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd17650    1 PDAIAVSDATRQ-----LTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAER 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIaLEPftvefekikeydaltkfviagqkdgwisleaekenasnklemadttrDDMAILSYTSGTTGN 191
Cdd:cd17650   76 LQYMLEDSGAKLLL-TQP-----------------------------------------------EDLAYVIYTSGTTGK 107
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAV--TH------CHGW---------GFAHLQMAPkhwlcINEDdlVWAtaapgwqkwvwSPFLSVLGSGATAFVF--N 252
Cdd:cd17650  108 PKGVmvEHrnvahaAHAWrreyeldsfPVRLLQMAS-----FSFD--VFA-----------GDFARSLLNGGTLVICpdE 169
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 253 GRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAV--SAGEPLN--REVVEQFKKYFNLTvrDGYGQ 328
Cdd:cd17650  170 VKLDPAALYDLILKSRITLMESTPALIRPVMAYVYRNGLDLSAMRLLIvgSDGCKAQdfKTLAARFGQGMRII--NSYGV 247
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 329 TESTLLIGFLKDTPQRIGS-----MGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDlpALFKGYYKEPERTAK------ 397
Cdd:cd17650  248 TEATIDSTYYEEGRDPLGDsanvpIGRPLPNTAMYVLDERLQPQPVGVAGELYIGGA--GVARGYLNRPELTAErfvenp 325
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 398 -AQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKEcAVVAKPHDIRGNIVKAFVILQDH 476
Cdd:cd17650  326 fAPGERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDE-AVVAVREDKGGEARLCAYVVAAA 404
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 612673656 477 TAgdDTlvKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd17650  405 TL--NT--AELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
PLN02479 PLN02479
acetate-CoA ligase
32-525 1.80e-26

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 113.40  E-value: 1.80e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGvAIIPCSEM-LRTK 110
Cdd:PLN02479  34 PTRKSVVH-----GSVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAG-AVVNCVNIrLNAP 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 111 DLQYRITHGEINAVIA-LEPFTVEFE--KIKEYDALTKF-----VIAGQKD------------GWISLEAEKENASNKLE 170
Cdd:PLN02479 108 TIAFLLEHSKSEVVMVdQEFFTLAEEalKILAEKKKSSFkppllIVIGDPTcdpkslqyalgkGAIEYEKFLETGDPEFA 187
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 171 MADTTRDDMAI-LSYTSGTTGNPKAVT--------------------------------HCHGWGFAHLQMApkhwLCIN 217
Cdd:PLN02479 188 WKPPADEWQSIaLGYTSGTTASPKGVVlhhrgaylmalsnaliwgmnegavylwtlpmfHCNGWCFTWTLAA----LCGT 263
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 218 EDDLVWATAapgwqKWVWSpflSVLGSGATAF-----VFNGRF-SP--ETYLELLQKYQINVLCCTPTEYRMMAklQNLN 289
Cdd:PLN02479 264 NICLRQVTA-----KAIYS---AIANYGVTHFcaapvVLNTIVnAPksETILPLPRVVHVMTAGAAPPPSVLFA--MSEK 333
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 290 DYDLTHLH--------SAVSAGEPLNRE--VVEQFKkyfnLTVRDGYgqtestlligflkdtpQRIGSMGkgipgssVTV 359
Cdd:PLN02479 334 GFRVTHTYglsetygpSTVCAWKPEWDSlpPEEQAR----LNARQGV----------------RYIGLEG-------LDV 386
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 360 VDDEGNS-VPANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPF 438
Cdd:PLN02479 387 VDTKTMKpVPADGKTMGEIVMRGNMVMKGYLKNPKANEEAFANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSL 466
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 439 EVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDD--TLVKELQQFVKNEVAPYKYPREIEFvDDLPKTNSG 516
Cdd:PLN02479 467 EVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSDeaALAEDIMKFCRERLPAYWVPKSVVF-GPLPKTATG 545

                 ....*....
gi 612673656 517 KIRRVELRD 525
Cdd:PLN02479 546 KIQKHVLRA 554
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
32-524 2.87e-26

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 112.39  E-value: 2.87e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTegediSVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPrSIAtyELYI---AALKLGVAIIPC----- 103
Cdd:PRK10946  37 SDAIAVICGER-----QFSYRELNQASDNLACSLRRQGIKPGDTALVQLG-NVA--EFYItffALLKLGVAPVNAlfshq 108
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 104 -SEM----------LRTKDLQYRITHGE--INAVIALEPftvefekikeydALTKFVIAGQkDGWISLEAEKENASNKLE 170
Cdd:PRK10946 109 rSELnayasqiepaLLIADRQHALFSDDdfLNTLVAEHS------------SLRVVLLLND-DGEHSLDDAINHPAEDFT 175
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 171 MADTTRDDMAILSYTSGTTGNPKAV--TH-------------ChgwGFAhlqmAPKHWLCINEDDLVWATAAPGwqkwvw 235
Cdd:PRK10946 176 ATPSPADEVAFFQLSGGSTGTPKLIprTHndyyysvrrsveiC---GFT----PQTRYLCALPAAHNYPMSSPG------ 242
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 236 spFLSVLGSGATAfVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMakLQNLND----YDLTHLHSAVSAGEPLNREVV 311
Cdd:PRK10946 243 --ALGVFLAGGTV-VLAPDPSATLCFPLIEKHQVNVTALVPPAVSLW--LQAIAEggsrAQLASLKLLQVGGARLSETLA 317
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 312 EQFKKYFNLTVRDGYGQTEStlLIGF--LKDTPQRI-GSMGKGI-PGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKG 387
Cdd:PRK10946 318 RRIPAELGCQLQQVFGMAEG--LVNYtrLDDSDERIfTTQGRPMsPDDEVWVADADGNPLPQGEVGRLMT--RGPYTFRG 393
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 388 YYKEPERTAKA-QTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNI 466
Cdd:PRK10946 394 YYKSPQHNASAfDANGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEK 473
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 612673656 467 VKAFVILQDHtagddtlVK--ELQQFVKNE-VAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK10946 474 SCAFLVVKEP-------LKavQLRRFLREQgIAEFKLPDRVECVDSLPLTAVGKVDKKQLR 527
PRK12467 PRK12467
peptide synthase; Provisional
50-533 3.04e-26

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 114.10  E-value: 3.04e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEP 129
Cdd:PRK12467 1601 TYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSH 1680
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  130 FTVEFEKIKEYDALtkfvIAGQKDGWISLEAEKENASNklemadTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQmA 209
Cdd:PRK12467 1681 LQARLPLPDGLRSL----VLDQEDDWLEGYSDSNPAVN------LAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLC-A 1749
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  210 PKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFV-FNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKL--Q 286
Cdd:PRK12467 1750 TQEAYQLSAADVVLQFTSFAFDVSVWELFWPLINGARLVIApPGAHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMdeQ 1829
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  287 NLNDYDLTHLhsaVSAGEPLNREVVEQ-FKKYFNLTVRDGYGQTESTLLIGF----LKDTPQRIGS-MGKGIPGSSVTVV 360
Cdd:PRK12467 1830 VEHPLSLRRV---VCGGEALEVEALRPwLERLPDTGLFNLYGPTETAVDVTHwtcrRKDLEGRDSVpIGQPIANLSTYIL 1906
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  361 DDEGNSVPANTKGNIAVPLDlpALFKGYYKEPERTAK-------AQTG-KYYITGDLAHVDDDGYFWFEGRRDDIIISSG 432
Cdd:PRK12467 1907 DASLNPVPIGVAGELYLGGV--GLARGYLNRPALTAErfvadpfGTVGsRLYRTGDLARYRADGVIEYLGRIDHQVKIRG 1984
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  433 YTIGPFEVEDALTNHPAVKECAVVAkpHDIR-GNIVKAFVILQDHTAGDD-----TLVKELQQFVKNEVAPYKYPREIEF 506
Cdd:PRK12467 1985 FRIELGEIEARLREQGGVREAVVIA--QDGAnGKQLVAYVVPTDPGLVDDdeaqvALRAILKNHLKASLPEYMVPAHLVF 2062
                         490       500
                  ....*....|....*....|....*..
gi 612673656  507 VDDLPKTNSGKIRRVELRDAEIEKWQQ 533
Cdd:PRK12467 2063 LARMPLTPNGKLDRKALPAPDASELQQ 2089
hsFATP2a_ACSVL_like cd05938
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ...
45-523 4.53e-24

Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341261 [Multi-domain]  Cd Length: 537  Bit Score: 105.84  E-value: 4.53e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  45 EDISVTYQQLIEQSNKVGNVLASH-GLSKGDKVLIMMPRSIATYELYIAALKLG--VAIIPCSemLRTKDLQYRITHGEI 121
Cdd:cd05938    2 EGETYTYRDVDRRSNQAARALLAHaGLRPGDTVALLLGNEPAFLWIWLGLAKLGcpVAFLNTN--IRSKSLLHCFRCCGA 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 122 NAVI-------ALEPFTVEFEKikeyDALTKFVIAGQK--DGWISLEAEKENASNKLEMAD----TTRDDMAILSYTSGT 188
Cdd:cd05938   80 KVLVvapelqeAVEEVLPALRA----DGVSVWYLSHTSntEGVISLLDKVDAASDEPVPASlrahVTIKSPALYIYTSGT 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 189 TGNPKA--VTHCHGWgfahlQMAPKHWLC-INEDDLVWATAapgwqkwvwsP----------FLSVLGSGATaFVFNGRF 255
Cdd:cd05938  156 TGLPKAarISHLRVL-----QCSGFLSLCgVTADDVIYITL----------PlyhssgfllgIGGCIELGAT-CVLKPKF 219
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 256 SPETYLELLQKYQINV----------LCCTPteyrmmaklQNLNDYDlthlHSA-VSAGEPLNREVVEQFKKYF-NLTVR 323
Cdd:cd05938  220 SASQFWDDCRKHNVTViqyigellryLCNQP---------QSPNDRD----HKVrLAIGNGLRADVWREFLRRFgPIRIR 286
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 324 DGYGQTESTllIGFLKDTpQRIGSMGKG-------IPGSSVTVVDDEGNSVPANTKGNIAVPLDLPAL----------FK 386
Cdd:cd05938  287 EFYGSTEGN--IGFFNYT-GKIGAVGRVsylykllFPFELIKFDVEKEEPVRDAQGFCIPVAKGEPGLlvakitqqspFL 363
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 387 GYYKEPERTAKA------QTGK-YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAV--VA 457
Cdd:cd05938  364 GYAGDKEQTEKKllrdvfKKGDvYFNTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLLDFLQEVNVygVT 443
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 612673656 458 KP-HDirGNIVKAFVILQDHTAGDdtlVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd05938  444 VPgHE--GRIGMAAVKLKPGHEFD---GKKLYQHVREYLPAYARPRFLRIQDSLEITGTFKQQKVRL 505
PRK07008 PRK07008
long-chain-fatty-acid--CoA ligase; Validated
182-525 9.46e-24

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235908 [Multi-domain]  Cd Length: 539  Bit Score: 104.79  E-value: 9.46e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 182 LSYTSGTTGNPKAVTHCHGWGFAHLQMA--PKHwLCINEDDLVWATAaPGWQKWVWSPFLSVLGSGATaFVFNG-RFSPE 258
Cdd:PRK07008 181 LCYTSGTTGNPKGALYSHRSTVLHAYGAalPDA-MGLSARDAVLPVV-PMFHVNAWGLPYSAPLTGAK-LVLPGpDLDGK 257
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 259 TYLELLQKYQINVLCCTPTEYRMMaklqnlndydLTHLHSAVSAGEPLNREVV----------EQFKKYFNLTVRDGYGQ 328
Cdd:PRK07008 258 SLYELIEAERVTFSAGVPTVWLGL----------LNHMREAGLRFSTLRRTVIggsacppamiRTFEDEYGVEVIHAWGM 327
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 329 TE-------STLLIGFLKDTP----QRIGSMGKGIPGSSVTVVDDEGNSVPANTK--GNIAVplDLPALFKGYYKepeRT 395
Cdd:PRK07008 328 TEmsplgtlCKLKWKHSQLPLdeqrKLLEKQGRVIYGVDMKIVGDDGRELPWDGKafGDLQV--RGPWVIDRYFR---GD 402
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 396 AKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPH---DIRGNIVkafVI 472
Cdd:PRK07008 403 ASPLVDGWFPTGDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHpkwDERPLLV---VV 479
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|...
gi 612673656 473 LQdhtAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK07008 480 KR---PGAEVTREELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKLRE 529
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
326-525 1.26e-23

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 103.54  E-value: 1.26e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 326 YGQTESTLLIGFLKdtPQRI----GSMGKGIPGSSVTVVddegnsvpANTKGNIAVplDLPALFKGYYkePERTAKAqtg 401
Cdd:PRK07445 261 YGMTETASQIATLK--PDDFlagnNSSGQVLPHAQITIP--------ANQTGNITI--QAQSLALGYY--PQILDSQ--- 323
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 402 KYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDhtagDD 481
Cdd:PRK07445 324 GIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKD----PS 399
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....
gi 612673656 482 TLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PRK07445 400 ISLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQ 443
FATP_FACS cd05940
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ...
50-524 1.27e-23

Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341263 [Multi-domain]  Cd Length: 449  Bit Score: 103.59  E-value: 1.27e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIipcsemlrtkdlqyrithgeinavialep 129
Cdd:cd05940    5 TYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVA----------------------------- 55
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 130 ftvefekikeydALTKFVIAGQkdgwiSLEAEKENASNKLEMADTtrddmAILSYTSGTTGNPKA--VTHCHGWGFAHLQ 207
Cdd:cd05940   56 ------------ALINYNLRGE-----SLAHCLNVSSAKHLVVDA-----ALYIYTSGTTGLPKAaiISHRRAWRGGAFF 113
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 208 MapkHWLCINEDDLVWAT-----AAPGWQKWVwspflSVLGSGATaFVFNGRFSPETYLELLQKYQinvlcCTPTEY--- 279
Cdd:cd05940  114 A---GSGGALPSDVLYTClplyhSTALIVGWS-----ACLASGAT-LVIRKKFSASNFWDDIRKYQ-----ATIFQYige 179
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 280 --RMMAKlQNLNDYDLTHLHSAVSaGEPLNREVVEQFKKYFNL-TVRDGYGQTESTllIGFLkDTPQRIGSMGKgIPGSS 356
Cdd:cd05940  180 lcRYLLN-QPPKPTERKHKVRMIF-GNGLRPDIWEEFKERFGVpRIAEFYAATEGN--SGFI-NFFGKPGAIGR-NPSLL 253
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 357 VTV-------VDDEGNSVPANTKGN-IAVPLDLPAL----------FKGYYKEPERTAK------AQTGKYYITGDLAHV 412
Cdd:cd05940  254 RKVaplalvkYDLESGEPIRDAEGRcIKVPRGEPGLlisrinplepFDGYTDPAATEKKilrdvfKKGDAWFNTGDLMRL 333
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 413 DDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAV--VAKP-HDirGNIVKAFVILQDHTAGDdtlVKELQQ 489
Cdd:cd05940  334 DGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVygVQVPgTD--GRAGMAAIVLQPNEEFD---LSALAA 408
                        490       500       510
                 ....*....|....*....|....*....|....*
gi 612673656 490 FVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd05940  409 HLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLR 443
AACS cd05943
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ...
32-523 1.53e-23

Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.


Pssm-ID: 341265 [Multi-domain]  Cd Length: 629  Bit Score: 104.66  E-value: 1.53e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCS-EMlrtk 110
Cdd:cd05943   82 ADDPAAIYAAEDGERTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSpDF---- 157
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 111 dlqyrithGeINAVIA----LEP---FTVE--FEKIKEYDALTK-------------------FVIAGQKDG-----WIS 157
Cdd:cd05943  158 --------G-VPGVLDrfgqIEPkvlFAVDayTYNGKRHDVREKvaelvkglpsllavvvvpyTVAAGQPDLskiakALT 228
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 158 LEAEK-ENASNKLEMADTTRDD-MAILsYTSGTTGNPKAVTHCHGWGFahLQMAPKHWLCIN---EDDLVWATaAPGWQK 232
Cdd:cd05943  229 LEDFLaTGAAGELEFEPLPFDHpLYIL-YSSGTTGLPKCIVHGAGGTL--LQHLKEHILHCDlrpGDRLFYYT-TCGWMM 304
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 233 WVWspFLSVLGSGATAFVFNG---RFSPETYLELLQKYQINVLCCTPTeYRMM---AKLQNLNDYDLTHLHSAVSAGEPL 306
Cdd:cd05943  305 WNW--LVSGLAVGATIVLYDGspfYPDTNALWDLADEEGITVFGTSAK-YLDAlekAGLKPAETHDLSSLRTILSTGSPL 381
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 307 NRE----VVEQFKKyfNLTVRDGYGQTE-STLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANtKGNIAVPLDL 381
Cdd:cd05943  382 KPEsfdyVYDHIKP--DVLLASISGGTDiISCFVGGNPLLPVYRGEIQCRGLGMAVEAFDEEGKPVWGE-KGELVCTKPF 458
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 382 PALFKGYYKEPE--RTAKAQTGKY---YITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVV 456
Cdd:cd05943  459 PSMPVGFWNDPDgsRYRAAYFAKYpgvWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVV 538
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 612673656 457 AKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKirRVEL 523
Cdd:cd05943  539 GQEWKDGDERVILFVKLREGVELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGK--KVEV 603
PRK05857 PRK05857
fatty acid--CoA ligase;
35-526 1.65e-23

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 103.93  E-value: 1.65e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  35 KAILYHNTEGEDiSVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEML--RTKDL 112
Cdd:PRK05857  29 EAIALRRCDGTS-ALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLpiAAIER 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 113 QYRIThgEINAVIALEPFTVEFEKIKE----YDALTKFVIAGQKDGWISLEAEKENASnklemADTTRDDMAILSYTSGT 188
Cdd:PRK05857 108 FCQIT--DPAAALVAPGSKMASSAVPEalhsIPVIAVDIAAVTRESEHSLDAASLAGN-----ADQGSEDPLAMIFTSGT 180
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 189 TGNPKAVTHCHGWGFAhlqmAPKhwlCINEDDLVWATAAPGwqKWVWSPfLSVLGSGATAFVFNGRF----------SPE 258
Cdd:PRK05857 181 TGEPKAVLLANRTFFA----VPD---ILQKEGLNWVTWVVG--ETTYSP-LPATHIGGLWWILTCLMhgglcvtggeNTT 250
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 259 TYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGeplNREVVEQFKKYFNLTVRDG--YGQTES---TL 333
Cdd:PRK05857 251 SLLEILTTNAVATTCLVPTLLSKLVSELKSANATVPSLRLVGYGG---SRAIAADVRFIEATGVRTAqvYGLSETgctAL 327
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 LIGFLKDTPQRI--GSMGKGIPGSSVTVVDDEGNSVPANTKGNIA----VPLDLPALFKGYYKEPERTAKAQTGKYYITG 407
Cdd:PRK05857 328 CLPTDDGSIVKIeaGAVGRPYPGVDVYLAATDGIGPTAPGAGPSAsfgtLWIKSPANMLGYWNNPERTAEVLIDGWVNTG 407
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 408 DLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQdhTAGDDTLVKEL 487
Cdd:PRK05857 408 DLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGALVGLAVVAS--AELDESAARAL 485
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|...
gi 612673656 488 QQFV----KNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDA 526
Cdd:PRK05857 486 KHTIaarfRRESEPMARPSTIVIVTDIPRTQSGKVMRASLAAA 528
PRK05691 PRK05691
peptide synthase; Validated
48-534 6.26e-21

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 97.55  E-value: 6.26e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   48 SVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRIthgEINAVIAL 127
Cdd:PRK05691 2213 TLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEYPLERLHYMI---EDSGIGLL 2289
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  128 EPFTVEFEKIKEYDA-LTKFVIagqKDGWISLEAEKENASNKLEMADttrdDMAILSYTSGTTGNPKAVTHCHGWGFAHL 206
Cdd:PRK05691 2290 LSDRALFEALGELPAgVARWCL---EDDAAALAAYSDAPLPFLSLPQ----HQAYLIYTSGSTGKPKGVVVSHGEIAMHC 2362
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  207 Q-------MAPK----HWLCINEDdlvwatAApgwQKWVWSPFLSvlgsGATAFV-FNGRFSPETYLELLQKYQINVLCC 274
Cdd:PRK05691 2363 QavierfgMRADdcelHFYSINFD------AA---SERLLVPLLC----GARVVLrAQGQWGAEEICQLIREQQVSILGF 2429
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  275 TPTEYRMMAklQNL-NDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVR-DGYGQTESTL--LIGFLKDT-PQRIGS-- 347
Cdd:PRK05691 2430 TPSYGSQLA--QWLaGQGEQLPVRMCITGGEALTGEHLQRIRQAFAPQLFfNAYGPTETVVmpLACLAPEQlEEGAASvp 2507
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  348 MGKGIPGSSVTVVDDEGNSVPANTKGNIAVplDLPALFKGYYKEPERTAK--------AQTGKYYITGDLAHVDDDGYFW 419
Cdd:PRK05691 2508 IGRVVGARVAYILDADLALVPQGATGELYV--GGAGLAQGYHDRPGLTAErfvadpfaADGGRLYRTGDLVRLRADGLVE 2585
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  420 FEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAkpHDI-RGNIVKAFVILQDHTAGDD---TLVKELQQFVKNEV 495
Cdd:PRK05691 2586 YVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVLA--LDTpSGKQLAGYLVSAVAGQDDEaqaALREALKAHLKQQL 2663
                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 612673656  496 APYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEKWQQQ 534
Cdd:PRK05691 2664 PDYMVPAHLILLDSLPLTANGKLDRRALPAPDPELNRQA 2702
PRK12316 PRK12316
peptide synthase; Provisional
32-533 1.29e-20

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 96.56  E-value: 1.29e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK12316 2017 PEAIAVVF-----GDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAER 2091
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  112 LQYRITHGEINAVIAlepftvefekikEYDALTKFVIAGqkdGWISL----EAEKENASNKLEMADTTRDDMAILSYTSG 187
Cdd:PRK12316 2092 LAYMLEDSGAALLLT------------QRHLLERLPLPA---GVARLpldrDAEWADYPDTAPAVQLAGENLAYVIYTSG 2156
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  188 TTGNPKAVTHCHGWGFAHLQMAPKHWLCINED-DLVWATAAPGWQKWVWSPFLSVlgsGATAFVFNGR-FSPETYLELLQ 265
Cdd:PRK12316 2157 STGLPKGVAVSHGALVAHCQAAGERYELSPADcELQFMSFSFDGAHEQWFHPLLN---GARVLIRDDElWDPEQLYDEME 2233
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  266 KYQINVLCCTPTEYRMMAKLQNLNDYDLThLHSAVSAGEPLNREVVEQFKK------YFNltvrdGYGQTESTLLIGFLK 339
Cdd:PRK12316 2234 RHGVTILDFPPVYLQQLAEHAERDGRPPA-VRVYCFGGEAVPAASLRLAWEalrpvyLFN-----GYGPTEAVVTPLLWK 2307
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  340 DTPQRIGS-----MGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAK--------AQTGKYYIT 406
Cdd:PRK12316 2308 CRPQDPCGaayvpIGRALGNRRAYILDADLNLLAPGMAGELY--LGGEGLARGYLNRPGLTAErfvpdpfsASGERLYRT 2385
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  407 GDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKpHDIRGNIVKAFVILQDhtaGDDTLVKE 486
Cdd:PRK12316 2386 GDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVVAQ-DGASGKQLVAYVVPDD---AAEDLLAE 2461
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*..
gi 612673656  487 LQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEIEKWQQ 533
Cdd:PRK12316 2462 LRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQLRQ 2508
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
49-456 1.58e-20

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 94.35  E-value: 1.58e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  49 VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSiatYELYIA---ALKLGVAIIPCSEMLRTKDLQYRITHGEINAVI 125
Cdd:cd17640    6 ITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNS---PRWLIAdqgIMALGAVDVVRGSDSSVEELLYILNHSESVALV 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 126 AlepftvefekikeydaltkfviagqkdgwisleaekENASnklemadttrDDMAILSYTSGTTGNPKAVTHCHGwGFAH 205
Cdd:cd17640   83 V------------------------------------ENDS----------DDLATIIYTSGTTGNPKGVMLTHA-NLLH 115
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 206 lQMapKHWLCIneddlvwATAAPGwqkwvwSPFLSVLGS------GATAFVFNGRF-----SPETYLELLQKYQINVLCC 274
Cdd:cd17640  116 -QI--RSLSDI-------VPPQPG------DRFLSILPIwhsyerSAEYFIFACGCsqaytSIRTLKDDLKRVKPHYIVS 179
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 275 TPteyRMMAKLQNlndydltHLHSAVSAGEPLNREVVEQFK--------------------KYFNLT---VRDGYGQTES 331
Cdd:cd17640  180 VP---RLWESLYS-------GIQKQVSKSSPIKQFLFLFFLsggifkfgisgggalpphvdTFFEAIgieVLNGYGLTET 249
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 332 TLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSV-PANTKGNIAVplDLPALFKGYYKEPERTAKAQTGK-YYITGDL 409
Cdd:cd17640  250 SPVVSARRLKCNVRGSVGRPLPGTEIKIVDPEGNVVlPPGEKGIVWV--RGPQVMKGYYKNPEATSKVLDSDgWFNTGDL 327
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....*...
gi 612673656 410 AHVDDDGYFWFEGR-RDDIIISSGYTIGPFEVEDALTNHPAVKECAVV 456
Cdd:cd17640  328 GWLTCGGELVLTGRaKDTIVLSNGENVEPQPIEEALMRSPFIEQIMVV 375
PRK03584 PRK03584
acetoacetate--CoA ligase;
32-523 2.41e-20

acetoacetate--CoA ligase;


Pssm-ID: 235134 [Multi-domain]  Cd Length: 655  Bit Score: 94.86  E-value: 2.41e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGvAI-IPCS-EMlrt 109
Cdd:PRK03584  98 DDRPAIIFRGEDGPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLG-AIwSSCSpDF--- 173
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 110 kdlqyrithGEiNAVIA----LEP---FTVEF-----------EKIKE----YDALTKFVI---------AGQKDGWISL 158
Cdd:PRK03584 174 ---------GV-QGVLDrfgqIEPkvlIAVDGyryggkafdrrAKVAElraaLPSLEHVVVvpylgpaaaAAALPGALLW 243
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 159 -EAEKENASNKLEMADTTRDD-MAILsYTSGTTGNPKAVTHCHGwG--FAHLQMAPKHWLCINEDDLVWATaAPGWQKWV 234
Cdd:PRK03584 244 eDFLAPAEAAELEFEPVPFDHpLWIL-YSSGTTGLPKCIVHGHG-GilLEHLKELGLHCDLGPGDRFFWYT-TCGWMMWN 320
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 235 WspFLSVLGSGATAFVFNGrfSP-----ETYLELLQKYQINVLCCTPTEYRMMAK--LQNLNDYDLTHLHSAVSAGEPLN 307
Cdd:PRK03584 321 W--LVSGLLVGATLVLYDG--SPfypdpNVLWDLAAEEGVTVFGTSAKYLDACEKagLVPGETHDLSALRTIGSTGSPLP 396
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 308 RE----VVEQFKKyfNLTVRDGYGQTE-STLLIGFLKDTPQRIGSM-GKGIpGSSVTVVDDEGNSVpANTKGNIAVPLDL 381
Cdd:PRK03584 397 PEgfdwVYEHVKA--DVWLASISGGTDiCSCFVGGNPLLPVYRGEIqCRGL-GMAVEAWDEDGRPV-VGEVGELVCTKPF 472
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 382 PALFKGYYKEPErtakaqtGKYYIT------------GDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPA 449
Cdd:PRK03584 473 PSMPLGFWNDPD-------GSRYRDayfdtfpgvwrhGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPE 545
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 612673656 450 VKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIrrVEL 523
Cdd:PRK03584 546 VLDSLVIGQEWPDGDVRMPLFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKK--VEL 617
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
147-524 2.54e-20

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 92.42  E-value: 2.54e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 147 VIAGQKDGWISLEAEKENASNKLemADTTR------DDMAILSYTSGTTGNPKAV-------------THCH--GWGFAH 205
Cdd:PRK07824   1 ALAGRAPALLPVPAQDERRAALL--RDALRvgepidDDVALVVATSGTTGTPKGAmltaaaltasadaTHDRlgGPGQWL 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 206 LQMAPKHwlcineddlvwataAPGWQKWVWSpflSVLGSGATAFVFNGRFSPETYLELLQKYQinvlccTPTEYRMMAKL 285
Cdd:PRK07824  79 LALPAHH--------------IAGLQVLVRS---VIAGSEPVELDVSAGFDPTALPRAVAELG------GGRRYTSLVPM 135
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 286 QNLNDYD-------LTHLHSAVSAGEPLNREVVEQFKKyFNLTVRDGYGQTESTLliGFLKDtpqrigsmGKGIPGSSVT 358
Cdd:PRK07824 136 QLAKALDdpaataaLAELDAVLVGGGPAPAPVLDAAAA-AGINVVRTYGMSETSG--GCVYD--------GVPLDGVRVR 204
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 359 VVDdegnsvpantkGNIAvpLDLPALFKGYYKEPERTAKAQTGkYYITGDLAHVDDdGYFWFEGRRDDIIISSGYTIGPF 438
Cdd:PRK07824 205 VED-----------GRIA--LGGPTLAKGYRNPVDPDPFAEPG-WFRTDDLGALDD-GVLTVLGRADDAISTGGLTVLPQ 269
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 439 EVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAgdDTLvKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKI 518
Cdd:PRK07824 270 VVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDGGPA--PTL-EALRAHVARTLDRTAAPRELHVVDELPRRGIGKV 346

                 ....*.
gi 612673656 519 RRVELR 524
Cdd:PRK07824 347 DRRALV 352
PRK08308 PRK08308
acyl-CoA synthetase; Validated
181-528 9.71e-20

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 91.64  E-value: 9.71e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 181 ILSYTSGTTGNPKAVTHchGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKW-VWSPFLSVLGSGATAFVFNGRfSPET 259
Cdd:PRK08308 105 LLQYSSGTTGEPKLIRR--SWTEIDREIEAYNEALNCEQDETPIVACPVTHSYgLICGVLAALTRGSKPVIITNK-NPKF 181
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 260 YLELLQKYQINVLCCTPTEYRMMAKLQNLNDydltHLHSAVSAGEPLNREVVEQFKKYFNLTVRDgYGQTEStlliGFLK 339
Cdd:PRK08308 182 ALNILRNTPQHILYAVPLMLHILGRLLPGTF----QFHAVMTSGTPLPEAWFYKLRERTTYMMQQ-YGCSEA----GCVS 252
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 340 DTPQ--RIGSMGKGIPGSSVTVVDDEGNsvpantkgniavpldlpalfkgyykePERTaKAQTGKYYI-TGDLAHVDDDG 416
Cdd:PRK08308 253 ICPDmkSHLDLGNPLPHVSVSAGSDENA--------------------------PEEI-VVKMGDKEIfTKDLGYKSERG 305
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 417 YFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVIlQDHTAGDDtlvkELQQFVKNEVA 496
Cdd:PRK08308 306 TLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVI-SHEEIDPV----QLREWCIQHLA 380
                        330       340       350
                 ....*....|....*....|....*....|..
gi 612673656 497 PYKYPREIEFVDDLPKTNSGKIRRVELRDAEI 528
Cdd:PRK08308 381 PYQVPHEIESVTEIPKNANGKVSRKLLELGEV 412
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
50-456 1.28e-19

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 91.75  E-value: 1.28e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQyrithgeiNAVIALEP 129
Cdd:cd05910    4 SFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKNLK--------QCLQEAEP 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 130 ftvefekikeydaltkfviagqkDGWISLEaekenasnkleMADttrDDMAILsYTSGTTGNPKAVTHCHGWGFAHLQmA 209
Cdd:cd05910   76 -----------------------DAFIGIP-----------KAD---EPAAIL-FTSGSTGTPKGVVYRHGTFAAQID-A 116
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 210 PKHWLCINEDDLVWATAAPgwqkwvWSPFLSVLGSGATAFVFN----GRFSPETYLELLQKYQINVLCCTPTEYRMMAKL 285
Cdd:cd05910  117 LRQLYGIRPGEVDLATFPL------FALFGPALGLTSVIPDMDptrpARADPQKLVGAIRQYGVSIVFGSPALLERVARY 190
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 286 QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFN-----LTVrdgYGQTEStLLIGFLKD-----TPQRIGSMGKG---- 351
Cdd:cd05910  191 CAQHGITLPSLRRVLSAGAPVPIALAARLRKMLSdeaeiLTP---YGATEA-LPVSSIGSrellaTTTAATSGGAGtcvg 266
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 352 --IPGSSVTVV--DDEG-------NSVPANTKGNIAVplDLPALFKGYYKEPERTAKAQT-----GKYYITGDLAHVDDD 415
Cdd:cd05910  267 rpIPGVRVRIIeiDDEPiaewddtLELPRGEIGEITV--TGPTVTPTYVNRPVATALAKIddnseGFWHRMGDLGYLDDE 344
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|.
gi 612673656 416 GYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVV 456
Cdd:cd05910  345 GRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSALV 385
hsFATP4_like cd05939
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ...
50-524 1.61e-19

Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341262 [Multi-domain]  Cd Length: 474  Bit Score: 91.33  E-value: 1.61e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIalep 129
Cdd:cd05939    5 TFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALI---- 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 130 ftvefekikeYDALTKfviagqkdgwisLEAEKENASNKLEMADTTrdDMAILSYTSGTTGNPKA--VTHchgwgFAHLQ 207
Cdd:cd05939   81 ----------FNLLDP------------LLTQSSTEPPSQDDVNFR--DKLFYIYTSGTTGLPKAavIVH-----SRYYR 131
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 208 MAP--KHWLCINEDDLVWA------TAApgwqkwvwspflSVLGSG-----ATAFVFNGRFSPETYLELLQKYQinvlcC 274
Cdd:cd05939  132 IAAgaYYAFGMRPEDVVYDclplyhSAG------------GIMGVGqallhGSTVVIRKKFSASNFWDDCVKYN-----C 194
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 275 TPTEY-----RMMAKlQNLNDYDLTH-LHSAVSAGepLNREVVEQFKKYFNLT-VRDGYGQTESTLLIGFLKDTPQRIGS 347
Cdd:cd05939  195 TIVQYigeicRYLLA-QPPSEEEQKHnVRLAVGNG--LRPQIWEQFVRRFGIPqIGEFYGATEGNSSLVNIDNHVGACGF 271
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 348 MGKgIPGS----SVTVVDDE---------GNSVPANTK------GNIaVPLDLPALFKGYYKEPERTAK------AQTGK 402
Cdd:cd05939  272 NSR-ILPSvypiRLIKVDEDtgelirdsdGLCIPCQPGepgllvGKI-IQNDPLRRFDGYVNEGATNKKiardvfKKGDS 349
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 403 YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAV--VAKPHdIRGNIVKAFVILQDHTAGD 480
Cdd:cd05939  350 AFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVygVEVPG-VEGRAGMAAIVDPERKVDL 428
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 612673656 481 DTLVKELQQfvknEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd05939  429 DRFSAVLAK----SLPPYARPQFIRLLPEVDKTGTFKLQKTDLQ 468
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
50-456 6.33e-19

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 89.84  E-value: 6.33e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGLSKGDKVLIMmPRSIATYELYIAALKLGVAI-IPCSEMLRTKDLQYRITHGEINAVIAle 128
Cdd:cd05932    8 TWGEVADKARRLAAALRALGLEPGSKIALI-SKNCAEWFITDLAIWMAGHIsVPLYPTLNPDTIRYVLEHSESKALFV-- 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 129 pftvefEKIKEYDAlTKFVIAGQ--------------KDGWISLEAEkenaSNKLEMADTTRDD-MAILSYTSGTTGNPK 193
Cdd:cd05932   85 ------GKLDDWKA-MAPGVPEGlisislpppsaancQYQWDDLIAQ----HPPLEERPTRFPEqLATLIYTSGTTGQPK 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 194 AVTHCHG-WGFAhLQMAPKHwLCINEDD--LVWATAAPGWQKwVWSPFLSVLGSGATAFVFngrfSPETYLELLQKYQIN 270
Cdd:cd05932  154 GVMLTFGsFAWA-AQAGIEH-IGTEENDrmLSYLPLAHVTER-VFVEGGSLYGGVLVAFAE----SLDTFVEDVQRARPT 226
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 271 VLCCTP---TEYRM-------MAKLQNL---------------NDYDLTHLHSAVSAGEPLNREVVEQFKKyFNLTVRDG 325
Cdd:cd05932  227 LFFSVPrlwTKFQQgvqdkipQQKLNLLlkipvvnslvkrkvlKGLGLDQCRLAGCGSAPVPPALLEWYRS-LGLNILEA 305
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 326 YGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEgnsvpantkgniAVPLDLPALFKGYYKEPERTAKAQTGKYYI 405
Cdd:cd05932  306 YGMTENFAYSHLNYPGRDKIGTVGNAGPGVEVRISEDG------------EILVRSPALMMGYYKDPEATAEAFTADGFL 373
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|...
gi 612673656 406 -TGDLAHVDDDGYFWFEGRRDDII-ISSGYTIGPFEVEDALTNHPAVKECAVV 456
Cdd:cd05932  374 rTGDKGELDADGNLTITGRVKDIFkTSKGKYVAPAPIENKLAEHDRVEMVCVI 426
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
177-524 7.83e-19

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 89.86  E-value: 7.83e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAVTHCHGwGFAHLQMAPKHWLCINEDDlVWATAAPGWQKWVWSPFLSVLGSGAtAFVFNGRFS 256
Cdd:PLN02860 172 DDAVLICFTSGTTGRPKGVTISHS-ALIVQSLAKIAIVGYGEDD-VYLHTAPLCHIGGLSSALAMLMVGA-CHVLLPKFD 248
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 257 PETYLELLQKYQINVLCCTPTeyrMMAKL-----QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTE 330
Cdd:PLN02860 249 AKAALQAIKQHNVTSMITVPA---MMADLisltrKSMTWKVFPSVRKILNGGGSLSSRLLPDAKKLFpNAKLFSAYGMTE 325
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 --STLLIGFLKD--------------------TPQRIGS-MGKGIPGSSVTVVDDEGNSVpantkGNIAVplDLPALFKG 387
Cdd:PLN02860 326 acSSLTFMTLHDptlespkqtlqtvnqtksssVHQPQGVcVGKPAPHVELKIGLDESSRV-----GRILT--RGPHVMLG 398
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 388 YYKEPERTAKAQTGKYYI-TGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNI 466
Cdd:PLN02860 399 YWGQNSETASVLSNDGWLdTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEM 478
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 612673656 467 VKAFVILQDH-TAGDDTLVKELQQFV-----------KNEVAPYKYPREI-EFVDDLPKTNSGKIRRVELR 524
Cdd:PLN02860 479 VVACVRLRDGwIWSDNEKENAKKNLTlssetlrhhcrEKNLSRFKIPKLFvQWRKPFPLTTTGKIRRDEVR 549
PRK05691 PRK05691
peptide synthase; Validated
46-534 8.41e-19

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 91.00  E-value: 8.41e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   46 DISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEInavi 125
Cdd:PRK05691 3743 DQQWSYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLLGMIVGSFKAGAGYLPLDPGLPAQRLQRIIELSRT---- 3818
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  126 alePFTVEFEKIKE--YDALTKFVIAGQKDGWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVThCHGWGF 203
Cdd:PRK05691 3819 ---PVLVCSAACREqaRALLDELGCANRPRLLVWEEVQAGEVASHNPGIYSGPDNLAYVIYTSGSTGLPKGVM-VEQRGM 3894
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  204 AHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSpFLSvlgsgatAFVFNGRFS---------PETYLELLQKYQINVLCC 274
Cdd:PRK05691 3895 LNNQLSKVPYLALSEADVIAQTASQSFDISVWQ-FLA-------APLFGARVEivpnaiahdPQGLLAHVQAQGITVLES 3966
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  275 TPTEY-RMMAklqnlNDYD-LTHLHSAVSAGEPLNREVVEQF-KKYFNLTVRDGYGQTESTLLIGFLK-DTPQRIGS--- 347
Cdd:PRK05691 3967 VPSLIqGMLA-----EDRQaLDGLRWMLPTGEAMPPELARQWlQRYPQIGLVNAYGPAECSDDVAFFRvDLASTRGSylp 4041
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  348 MGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLdlPALFKGYYKEPERTAKA-------QTG-KYYITGDLAHVDDDGYFW 419
Cdd:PRK05691 4042 IGSPTDNNRLYLLDEALELVPLGAVGELCVAG--TGVGRGYVGDPLRTALAfvphpfgAPGeRLYRTGDLARRRSDGVLE 4119
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  420 FEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKpHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYK 499
Cdd:PRK05691 4120 YVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVAVQ-EGVNGKHLVGYLVPHQTVLAQGALLERIKQRLRAELPDYM 4198
                         490       500       510
                  ....*....|....*....|....*....|....*
gi 612673656  500 YPREIEFVDDLPKTNSGKIRRVELRDAEIEKWQQQ 534
Cdd:PRK05691 4199 VPLHWLWLDRLPLNANGKLDRKALPALDIGQLQSQ 4233
PRK05691 PRK05691
peptide synthase; Validated
32-535 9.51e-18

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 87.53  E-value: 9.51e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   32 PDKKAILYhntEGEdiSVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPC-------- 103
Cdd:PRK05691 1145 PERIALVW---DGG--SLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLdpdypaer 1219
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  104 -SEMLRTKDLQYRITHGEInavialepftveFEKIKEYDALTKFVIAGQK-DGWISleaekenASNKLEMADttrDDMAI 181
Cdd:PRK05691 1220 lAYMLADSGVELLLTQSHL------------LERLPQAEGVSAIALDSLHlDSWPS-------QAPGLHLHG---DNLAY 1277
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  182 LSYTSGTTGNPKAVthchgwGFAHLQMAPK-HWL----CINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNGRF- 255
Cdd:PRK05691 1278 VIYTSGSTGQPKGV------GNTHAALAERlQWMqatyALDDSDVLMQKAPISFDVSVWECFWPLITGCRLVLAGPGEHr 1351
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  256 SPETYLELLQKYQINVLCCTPTEYRMMakLQNLNDYDLTHLHSAVSAGEPLNRE----VVEQFKkyfNLTVRDGYGQTES 331
Cdd:PRK05691 1352 DPQRIAELVQQYGVTTLHFVPPLLQLF--IDEPLAAACTSLRRLFSGGEALPAElrnrVLQRLP---QVQLHNRYGPTET 1426
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  332 TLLIGFL---KDTPQRiGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPERTAK-------AQTG 401
Cdd:PRK05691 1427 AINVTHWqcqAEDGER-SPIGRPLGNVLCRVLDAELNLLPPGVAGELC--IGGAGLARGYLGRPALTAErfvpdplGEDG 1503
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  402 -KYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVIlqdhTAGD 480
Cdd:PRK05691 1504 aRLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQARLLAQPGVAQAAVLVREGAAGAQLVGYYTG----EAGQ 1579
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 612673656  481 DTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDAEiekWQQQK 535
Cdd:PRK05691 1580 EAEAERLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRRALPEPV---WQQRE 1631
PLN03052 PLN03052
acetate--CoA ligase; Provisional
33-525 6.21e-17

acetate--CoA ligase; Provisional


Pssm-ID: 215553 [Multi-domain]  Cd Length: 728  Bit Score: 83.98  E-value: 6.21e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  33 DKKAILYHNtEGEDIS----VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLR 108
Cdd:PLN03052 190 DSIAIIWRD-EGSDDLpvnrMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVVSIADSFA 268
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 109 TKDLQYRITHGEINAVialepFTVEF-----------EKIKEYDALTKFVIAGqKDGWISLEAEKENAS--NKLEMADTT 175
Cdd:PLN03052 269 PSEIATRLKISKAKAI-----FTQDVivrggksiplySRVVEAKAPKAIVLPA-DGKSVRVKLREGDMSwdDFLARANGL 342
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 176 RDD-------MAILSYT-----SGTTGNPKAV--TH-------CHGWgfAHLQMAPKHWLCineddlvWATAApGWQKWV 234
Cdd:PLN03052 343 RRPdeykaveQPVEAFTnilfsSGTTGEPKAIpwTQltplraaADAW--AHLDIRKGDIVC-------WPTNL-GWMMGP 412
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 235 WSPFLSVLgSGATAFVFNGrfSP--ETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREvve 312
Cdd:PLN03052 413 WLVYASLL-NGATLALYNG--SPlgRGFAKFVQDAKVTMLGTVPSIVKTWKNTNCMAGLDWSSIRCFGSTGEASSVD--- 486
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 313 qfkKYFNLTVRDGY-------GQTE--STLLIGFLKDtPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDlPA 383
Cdd:PLN03052 487 ---DYLWLMSRAGYkpiieycGGTElgGGFVTGSLLQ-PQAFAAFSTPAMGCKLFILDDSGNPYPDDAPCTGELALF-PL 561
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 384 LF------------KGYYK-EPERTAKaQTGKYyitGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDAL-TNHPA 449
Cdd:PLN03052 562 MFgasstllnadhyKVYFKgMPVFNGK-ILRRH---GDIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADES 637
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 450 VKECAVVAKPHDIRG--NIVkAFVILQDHTAGDDTLvKELQQFVKNEVA----PYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:PLN03052 638 VLETAAIGVPPPGGGpeQLV-IAAVLKDPPGSNPDL-NELKKIFNSAIQkklnPLFKVSAVVIVPSFPRTASNKVMRRVL 715

                 ..
gi 612673656 524 RD 525
Cdd:PLN03052 716 RQ 717
FCS cd05921
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ...
49-459 9.89e-17

Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.


Pssm-ID: 341245 [Multi-domain]  Cd Length: 561  Bit Score: 83.25  E-value: 9.89e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  49 VTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCS--------EMLRTKDLQYRITHGE 120
Cdd:cd05921   26 VTYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVSpayslmsqDLAKLKHLFELLKPGL 105
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 121 INAVIAlEPFTVEFEKIKEyDALTKFVIAGQKDGWISLEAEKENASNKLEMAD-----TTRDDMAILSYTSGTTGNPKAV 195
Cdd:cd05921  106 VFAQDA-APFARALAAIFP-LGTPLVVSRNAVAGRGAISFAELAATPPTAAVDaafaaVGPDTVAKFLFTSGSTGLPKAV 183
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 196 THCHGWGFAHLQMAPKHWLCINEDDLVWATAAPgwqkwvWS-------PFLSVLGSGATAFVFNGRFSP---ETYLELLQ 265
Cdd:cd05921  184 INTQRMLCANQAMLEQTYPFFGEEPPVLVDWLP------WNhtfggnhNFNLVLYNGGTLYIDDGKPMPggfEETLRNLR 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 266 KYQINVLCCTPTEYRM----MAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKyfnLTVRD---------GYGQTEST 332
Cdd:cd05921  258 EISPTVYFNVPAGWEMlvaaLEKDEALRRRFFKRLKLMFYAGAGLSQDVWDRLQA---LAVATvgeripmmaGLGATETA 334
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 333 LLIGFLKDTPQRIGSMGKGIPGSSVTVvddegnsVPANTKgnIAVPLDLPALFKGYYKEPERTAKA--QTGkYYITGDLA 410
Cdd:cd05921  335 PTATFTHWPTERSGLIGLPAPGTELKL-------VPSGGK--YEVRVKGPNVTPGYWRQPELTAQAfdEEG-FYCLGDAA 404
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 612673656 411 H-VDDD----GyFWFEGR-RDDIIISSG--YTIGPFEVEDALTNHPAVKEcAVVAKP 459
Cdd:cd05921  405 KlADPDdpakG-LVFDGRvAEDFKLASGtwVSVGPLRARAVAACAPLVHD-AVVAGE 459
A_NRPS_MycA_like cd05908
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ...
35-524 3.63e-16

The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341234 [Multi-domain]  Cd Length: 499  Bit Score: 81.00  E-value: 3.63e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  35 KAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPcsemlrtkdlqy 114
Cdd:cd05908    2 EGIIFILGDKKEKFVSYRHLREEALGYLGALQELGIKPGQEVVFQITHNNKFLYLFWACLLGGMIAVP------------ 69
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 115 rITHGEINAVIalepftveFEKIKEYDALTK-FVIAgqkdgwiSLEAEKENAsnklemadttrDDMAILSYTSGTTGNPK 193
Cdd:cd05908   70 -VSIGSNEEHK--------LKLNKVWNTLKNpYLIT-------EEEVLCELA-----------DELAFIQFSSGSTGDPK 122
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 194 AVTHCHGwGFAHLQMAPKHWLCINEDD--LVWATAAPGWQkwVWSPFLSVLGSGATAFVFNGRF---SPETYLELLQKYQ 268
Cdd:cd05908  123 GVMLTHE-NLVHNMFAILNSTEWKTKDriLSWMPLTHDMG--LIAFHLAPLIAGMNQYLMPTRLfirRPILWLKKASEHK 199
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 269 INVLCCTPTEYRMMAKLQN---LNDYDLTHLHSAVSAGEPLNREVVEQFKKY---FNL---TVRDGYGQTEST------- 332
Cdd:cd05908  200 ATIVSSPNFGYKYFLKTLKpekANDWDLSSIRMILNGAEPIDYELCHEFLDHmskYGLkrnAILPVYGLAEASvgaslpk 279
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 333 --------------LLIG-----FLKDTPQ--RIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAvpLDLPALFKGYYKE 391
Cdd:cd05908  280 aqspfktitlgrrhVTHGepepeVDKKDSEclTFVEVGKPIDETDIRICDEDNKILPDGYIGHIQ--IRGKNVTPGYYNN 357
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 392 PERTAKAQTGK-YYITGDLAHVDDdGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA---KPHDIRGNIV 467
Cdd:cd05908  358 PEATAKVFTDDgWLKTGDLGFIRN-GRLVITGREKDIIFVNGQNVYPHDIERIAEELEGVELGRVVAcgvNNSNTRNEEI 436
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 612673656 468 KAFVIlqdHTAGDDTLVKeLQQFVKNEVAPYKYPR--EIEFVDDLPKTNSGKIRRVELR 524
Cdd:cd05908  437 FCFIE---HRKSEDDFYP-LGKKIKKHLNKRGGWQinEVLPIRRIPKTTSGKVKRYELA 491
PRK06334 PRK06334
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
73-423 4.28e-16

long chain fatty acid--[acyl-carrier-protein] ligase; Validated


Pssm-ID: 180533 [Multi-domain]  Cd Length: 539  Bit Score: 81.02  E-value: 4.28e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  73 GDKVLIMMPRSIATYELYIAALKLGV--AIIPCSEMLRtkDLQYRITHGEINAVIALEPFTVEFEKIK----EYDALTKF 146
Cdd:PRK06334  67 DQHIGIMMPASAGAYIAYFATLLSGKipVMINWSQGLR--EVTACANLVGVTHVLTSKQLMQHLAQTHgedaEYPFSLIY 144
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 147 V--IAGQKDGW------ISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHlQMAPKHWLCINE 218
Cdd:PRK06334 145 MeeVRKELSFWekcrigIYMSIPFEWLMRWFGVSDKDPEDVAVILFTSGTEKLPKGVPLTHANLLAN-QRACLKFFSPKE 223
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 219 DDLVWATAAP----GWQKWVWSPFLSVLgsgATAFVFNgRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLT 294
Cdd:PRK06334 224 DDVMMSFLPPfhayGFNSCTLFPLLSGV---PVVFAYN-PLYPKKIVEMIDEAKVTFLGSTPVFFDYILKTAKKQESCLP 299
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 295 HLHSAVSAGEPLNREVVEQFKKYF-NLTVRDGYGQTESTLLIGF-LKDTPQRIGSMGKGIPGSSVTVVDDEgNSVPANTK 372
Cdd:PRK06334 300 SLRFVVIGGDAFKDSLYQEALKTFpHIQLRQGYGTTECSPVITInTVNSPKHESCVGMPIRGMDVLIVSEE-TKVPVSSG 378
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|...
gi 612673656 373 GNIAVPLDLPALFKGYYKEPERTAKAQTG--KYYITGDLAHVDDDGYFWFEGR 423
Cdd:PRK06334 379 ETGLVLTRGTSLFSGYLGEDFGQGFVELGgeTWYVTGDLGYVDRHGELFLKGR 431
FATP_chFAT1_like cd05937
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ...
177-530 5.90e-15

Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.


Pssm-ID: 341260 [Multi-domain]  Cd Length: 468  Bit Score: 77.09  E-value: 5.90e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 177 DDMAILSYTSGTTGNPKAV------THCHGWGFAH-LQMAP--KHWLCInedDLVWATAApgwqkwvWSPFLSVLGSGAT 247
Cdd:cd05937   87 DDPAILIYTSGTTGLPKAAaiswrrTLVTSNLLSHdLNLKNgdRTYTCM---PLYHGTAA-------FLGACNCLMSGGT 156
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 248 aFVFNGRFSPET--------------YLELLQKYQINVlccTPTEYRMMAKLQnlndydlthlhsaVSAGEPLNREVVEQ 313
Cdd:cd05937  157 -LALSRKFSASQfwkdvrdsgatiiqYVGELCRYLLST---PPSPYDRDHKVR-------------VAWGNGLRPDIWER 219
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 314 FKKYFNL-TVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIP------GSSVTVV--DDEGNSV------------PANTK 372
Cdd:cd05937  220 FRERFNVpEIGEFYAATEGVFALTNHNVGDFGAGAIGHHGLirrwkfENQVVLVkmDPETDDPirdpktgfcvraPVGEP 299
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 373 GNI--AVPLDLPALFKGYYKEPERTAKA------QTGK-YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDA 443
Cdd:cd05937  300 GEMlgRVPFKNREAFQGYLHNEDATESKlvrdvfRKGDiYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADV 379
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 444 LTNHPAVKECAV--VAKP-HDirGNIVKAFVILQDHTAGDDTLVK-ELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIR 519
Cdd:cd05937  380 LGAHPDIAEANVygVKVPgHD--GRAGCAAITLEESSAVPTEFTKsLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQ 457
                        410
                 ....*....|.
gi 612673656 520 RVELRDAEIEK 530
Cdd:cd05937  458 KGVLRDEGVDP 468
A_NRPS_acs4 cd17654
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ...
32-523 6.19e-15

acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341309 [Multi-domain]  Cd Length: 449  Bit Score: 77.13  E-value: 6.19e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYhNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:cd17654    1 PDRPALII-DQTTSDTTVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAAYAPIDPASPEQR 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRIThgeinavialepftvefekikeyDALTKFVIAGQKDGwislEAEKENASNKLEMADTTRDDMAILSYTSGTTGN 191
Cdd:cd17654   80 SLTVMK-----------------------KCHVSYLLQNKELD----NAPLSFTPEHRHFNIRTDECLAYVIHTSGTTGT 132
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PK--AVTH-CHGWGFAHLQMAPKhwlcINEDDLVWATAAPGWQKWVWSPFLSVLgSGATAFVfngrfSPETYLELLQKY- 267
Cdd:cd17654  133 PKivAVPHkCILPNIQHFRSLFN----ITSEDILFLTSPLTFDPSVVEIFLSLS-SGATLLI-----VPTSVKVLPSKLa 202
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 268 -------QINVLCCTPTEYRMMAK--LQNLNDYDLTHLHSAVSAGEPLNREVV-EQFKKYFNLT-VRDGYGQTE----ST 332
Cdd:cd17654  203 dilfkrhRITVLQATPTLFRRFGSqsIKSTVLSATSSLRVLALGGEPFPSLVIlSSWRGKGNRTrIFNIYGITEvscwAL 282
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 333 LLIGFLKDTPQRIGSmgkGIPGSSVTVVDDEGNSVPANtkgniavpLDLPALFKGYY-KEPERTAKAQtgkYYITGDLAH 411
Cdd:cd17654  283 AYKVPEEDSPVQLGS---PLLGTVIEVRDQNGSEGTGQ--------VFLGGLNRVCIlDDEVTVPKGT---MRATGDFVT 348
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 412 VDDDGYFwFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDirgNIVkAFVILQDHtagDDTLVKELQqfv 491
Cdd:cd17654  349 VKDGELF-FLGRKDSQIKRRGKRINLDLIQQVIESCLGVESCAVTLSDQQ---RLI-AFIVGESS---SSRIHKELQ--- 417
                        490       500       510
                 ....*....|....*....|....*....|..
gi 612673656 492 KNEVAPYKYPREIEFVDDLPKTNSGKIRRVEL 523
Cdd:cd17654  418 LTLLSSHAIPDTFVQIDKLPLTSHGKVDKSEL 449
PRK05620 PRK05620
long-chain fatty-acid--CoA ligase;
33-524 7.30e-15

long-chain fatty-acid--CoA ligase;


Pssm-ID: 180167 [Multi-domain]  Cd Length: 576  Bit Score: 77.52  E-value: 7.30e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  33 DKKAILYHNTEGEDIsvTYQQLIEQSNKVGNVLASH-GLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK05620  25 DTTVTTWGGAEQEQT--TFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQ 102
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEiNAVIALEPFTVE--FEKIKEYDALTKFVIAGQKDGWI------------SLEAEKENASNKLEMADTTRD 177
Cdd:PRK05620 103 IVHIINHAE-DEVIVADPRLAEqlGEILKECPCVRAVVFIGPSDADSaaahmpegikvySYEALLDGRSTVYDWPELDET 181
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 178 DMAILSYTSGTTGNPKAVTHCHGWGFAH-LQMAPKHWLCInEDDLVWATAAPGWQKWVWSPFLSVLGSGaTAFVFNGR-F 255
Cdd:PRK05620 182 TAAAICYSTGTTGAPKGVVYSHRSLYLQsLSLRTTDSLAV-THGESFLCCVPIYHVLSWGVPLAAFMSG-TPLVFPGPdL 259
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 256 SPETYLELLQKYQINVLCCTPTEYrmmakLQNLNDYDLTH-----LHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTE 330
Cdd:PRK05620 260 SAPTLAKIIATAMPRVAHGVPTLW-----IQLMVHYLKNPpermsLQEIYVGGSAVPPILIKAWEERYGVDVVHVWGMTE 334
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 sTLLIGFLKDTPQ----------RIgSMGKGIPGSSVTVVDDeGNSVPANTKGNIAVPLDLPALFKGYYKEP--ERTAKA 398
Cdd:PRK05620 335 -TSPVGTVARPPSgvsgearwayRV-SQGRFPASLEYRIVND-GQVMESTDRNEGEIQVRGNWVTASYYHSPteEGGGAA 411
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 399 QTGK---------------YYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIR 463
Cdd:PRK05620 412 STFRgedvedandrftadgWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKW 491
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 612673656 464 GNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK05620 492 GERPLAVTVLAPGIEPTRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLR 552
ACSBG_like cd05933
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ...
180-453 7.75e-14

Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341256 [Multi-domain]  Cd Length: 596  Bit Score: 74.32  E-value: 7.75e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 180 AILSYTSGTTGNPKAVTHCHG---W----GFAHLQMAPK--------------H--------WLCINEDDLVWaTAAPGW 230
Cdd:cd05933  153 CTLIYTSGTTGMPKGVMLSHDnitWtakaASQHMDLRPAtvgqesvvsylplsHiaaqildiWLPIKVGGQVY-FAQPDA 231
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 231 QKW-------------------VWSPFLSVLGSGAT--------AFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA 283
Cdd:cd05933  232 LKGtlvktlrevrptafmgvprVWEKIQEKMKAVGAksgtlkrkIASWAKGVGLETNLKLMGGESPSPLFYRLAKKLVFK 311
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 284 KLQNLNDYDltHLHSAVSAGEPLNREVveqfKKYF---NLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVV 360
Cdd:cd05933  312 KVRKALGLD--RCQKFFTGAAPISRET----LEFFlslNIPIMELYGMSETSGPHTISNPQAYRLLSCGKALPGCKTKIH 385
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 361 --DDEGNsvpantkGNIAVPLDLpaLFKGYYKEPERTAKA-QTGKYYITGDLAHVDDDGYFWFEGRRDDIII-SSGYTIG 436
Cdd:cd05933  386 npDADGI-------GEICFWGRH--VFMGYLNMEDKTEEAiDEDGWLHSGDLGKLDEDGFLYITGRIKELIItAGGENVP 456
                        330
                 ....*....|....*..
gi 612673656 437 PFEVEDaltnhpAVKEC 453
Cdd:cd05933  457 PVPIED------AVKKE 467
PRK09192 PRK09192
fatty acyl-AMP ligase;
39-536 2.82e-13

fatty acyl-AMP ligase;


Pssm-ID: 236403 [Multi-domain]  Cd Length: 579  Bit Score: 72.35  E-value: 2.82e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  39 YHNTEGEDISV-TYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGV--AIIPCSEMLRTKD---- 111
Cdd:PRK09192  39 FYDRRGQLEEAlPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGLvpVPLPLPMGFGGREsyia 118
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 -LQYRITHGEINAVIA---LEPFTVEfekikeydaltkfvIAGQKDGWISLEAEKENAsnkLEMADT-----TRDDMAIL 182
Cdd:PRK09192 119 qLRGMLASAQPAAIITpdeLLPWVNE--------------ATHGNPLLHVLSHAWFKA---LPEADValprpTPDDIAYL 181
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 183 SYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAtaapgwqkwvWSPF----------LSVLGSGATA-FVF 251
Cdd:PRK09192 182 QYSSGSTRFPRGVIITHRALMANLRAISHDGLKVRPGDRCVS----------WLPFyhdmglvgflLTPVATQLSVdYLP 251
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 252 NGRFS--PETYLELLQKYQINVLCCTPTEYRMMAK---LQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDG- 325
Cdd:PRK09192 252 TRDFArrPLQWLDLISRNRGTISYSPPFGYELCARrvnSKDLAELDLSCWRVAGIGADMIRPDVLHQFAEAFAPAGFDDk 331
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 326 -----YGQTESTLLIGF-------------------------LKDTPQRIGSM---GKGIPGSSVTVVDDEGNSVPANTK 372
Cdd:PRK09192 332 afmpsYGLAEATLAVSFsplgsgivveevdrdrleyqgkavaPGAETRRVRTFvncGKALPGHEIEIRNEAGMPLPERVV 411
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 373 GNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDdGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVK- 451
Cdd:PRK09192 412 GHICV--RGPSLMSGYFRDEESQDVLAADGWLDTGDLGYLLD-GYLYITGRAKDLIIINGRNIWPQDIEWIAEQEPELRs 488
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 452 -ECAVVAKPHDIRGNIVkafVILQDHTAGDDT---LVKELQQFVKN--------EVAPykyPREiefvddLPKTNSGKIR 519
Cdd:PRK09192 489 gDAAAFSIAQENGEKIV---LLVQCRISDEERrgqLIHALAALVRSefgveaavELVP---PHS------LPRTSSGKLS 556
                        570
                 ....*....|....*..
gi 612673656 520 RVELRDAEIEKWQQQKD 536
Cdd:PRK09192 557 RAKAKKRYLSGAFASLD 573
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
32-520 6.96e-13

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 71.61  E-value: 6.96e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   32 PDKKAILYhntegEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCsemlrtkD 111
Cdd:PRK10252  472 PDAPALAD-----ARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPL-------D 539
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  112 LQY---RIthgeinAVIaLEpftvefekikeyDALTKFVIAGQK------DGWISLEAEKENASNKLEMADTTR---DDM 179
Cdd:PRK10252  540 TGYpddRL------KMM-LE------------DARPSLLITTADqlprfaDVPDLTSLCYNAPLAPQGAAPLQLsqpHHT 600
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  180 AILSYTSGTTGNPKAVTHCHG-------WGFAHLQMAPkhwlcineDDLVWATAAPGWQKWVWSPFLSVLgSGATAFVF- 251
Cdd:PRK10252  601 AYIIFTSGSTGRPKGVMVGQTaivnrllWMQNHYPLTA--------DDVVLQKTPCSFDVSVWEFFWPFI-AGAKLVMAe 671
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  252 -NGRFSPETYLELLQKYQINVLCCTPTeyrMMAKL------QNLNDYDLThLHSAVSAGEPLNREVVEQFKKYFNLTVRD 324
Cdd:PRK10252  672 pEAHRDPLAMQQFFAEYGVTTTHFVPS---MLAAFvasltpEGARQSCAS-LRQVFCSGEALPADLCREWQQLTGAPLHN 747
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  325 GYGQTESTLLIGFLKDTPQRIGSmgkgIPGSSVT-----------VVDDEGNSVPANTKGNIAvpLDLPALFKGYYKEPE 393
Cdd:PRK10252  748 LYGPTEAAVDVSWYPAFGEELAA----VRGSSVPigypvwntglrILDARMRPVPPGVAGDLY--LTGIQLAQGYLGRPD 821
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  394 RTAK-------AQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKphdirgni 466
Cdd:PRK10252  822 LTASrfiadpfAPGERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHAC-------- 893
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 612673656  467 vkafVILQDHTAGDDT------LVKE---------LQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRR 520
Cdd:PRK10252  894 ----VINQAAATGGDArqlvgyLVSQsglpldtsaLQAQLRERLPPHMVPVVLLQLDQLPLSANGKLDR 958
PRK05851 PRK05851
long-chain-fatty acid--ACP ligase MbtM;
180-524 7.47e-13

long-chain-fatty acid--ACP ligase MbtM;


Pssm-ID: 180289 [Multi-domain]  Cd Length: 525  Bit Score: 70.95  E-value: 7.47e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 180 AILSYTSGTTGNPK-------AVTHCHGWGFAHLQMAPKH-----WLCINED----DLVWA--TAAPGWQkwvwSPflsv 241
Cdd:PRK05851 155 AVLQGTAGSTGTPRtailspgAVLSNLRGLNARVGLDAATdvgcsWLPLYHDmglaFLLTAalAGAPLWL----AP---- 226
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 242 lgsgATAFVFngrfSPETYLELLQKYQINVLCCTPTEYRMMAKL-QNLNDYDLTHLHSAVSAGEPLNrevVEQFKKYFNL 320
Cdd:PRK05851 227 ----TTAFSA----SPFRWLSWLSDSRATLTAAPNFAYNLIGKYaRRVSDVDLGALRVALNGGEPVD---CDGFERFATA 295
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 321 TVRDG---------YGQTESTLLI-------GFLKD--------TPQRIGSMGKGIPGSSVTVVDDEGnSVPANTKGNIA 376
Cdd:PRK05851 296 MAPFGfdagaaapsYGLAESTCAVtvpvpgiGLRVDevttddgsGARRHAVLGNPIPGMEVRISPGDG-AAGVAGREIGE 374
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 377 VPLDLPALFKGYYKEpertAKAQTGKYYITGDLAHVDDDGYFwFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVV 456
Cdd:PRK05851 375 IEIRGASMMSGYLGQ----APIDPDDWFPTGDLGYLVDGGLV-VCGRAKELITVAGRNIFPTEIERVAAQVRGVREGAVV 449
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 612673656 457 AKPHDiRGNIVKAFVILQDHTAGD-DTLVKELQQFVKNEVApyKYPREIEFVD--DLPKTNSGKIRRVELR 524
Cdd:PRK05851 450 AVGTG-EGSARPGLVIAAEFRGPDeAGARSEVVQRVASECG--VVPSDVVFVApgSLPRTSSGKLRRLAVK 517
PRK08043 PRK08043
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
45-524 3.34e-12

bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;


Pssm-ID: 181207 [Multi-domain]  Cd Length: 718  Bit Score: 68.97  E-value: 3.34e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  45 EDISV---TYQQLIEQSNKVGNVLASHGlSKGDKVLIMMPRSIATyelyiAALKLGVAI---IPCseMLR----TKDLQY 114
Cdd:PRK08043 225 EDVNFtpdSYRKLLKKTLFVGRILEKYS-VEGERIGLMLPNATIS-----AAVIFGASLrrrIPA--MMNytagVKGLTS 296
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 115 RITHGEINAVIALEPFtveFEKIKeydaLTKFVIAGQKDGWISLEAEKEN--ASNKL-----------EMADTTRDDMAI 181
Cdd:PRK08043 297 AITAAEIKTIFTSRQF---LDKGK----LWHLPEQLTQVRWVYLEDLKDDvtTADKLwifahllmprlAQVKQQPEDAAL 369
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 182 LSYTSGTTGNPKAVTHCHGWGFAHLQ-------MAPKhwlcineDDLVWATA---APGWQKWVWSPFLSvlgsGATAFVF 251
Cdd:PRK08043 370 ILFTSGSEGHPKGVVHSHKSLLANVEqiktiadFTPN-------DRFMSALPlfhSFGLTVGLFTPLLT----GAEVFLY 438
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 252 NgrfSPETYL---ELLQKYQINVLCCTPTEYRMMAKLQNlnDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQ 328
Cdd:PRK08043 439 P---SPLHYRivpELVYDRNCTVLFGTSTFLGNYARFAN--PYDFARLRYVVAGAEKLQESTKQLWQDKFGLRILEGYGV 513
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 329 TESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVddegnSVPANTKGNiAVPLDLPALFKGYYK-------EPERTAKAQ-- 399
Cdd:PRK08043 514 TECAPVVSINVPMAAKPGTVGRILPGMDARLL-----SVPGIEQGG-RLQLKGPNIMNGYLRvekpgvlEVPTAENARge 587
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 400 --TGkYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVED-ALTNHPAvKECAVVAKPHDIRGnivKAFVIL-QD 475
Cdd:PRK08043 588 meRG-WYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQlALGVSPD-KQHATAIKSDASKG---EALVLFtTD 662
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*....
gi 612673656 476 HTAGDDTLVKELQQFVKNEVApykYPREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK08043 663 SELTREKLQQYAREHGVPELA---VPRDIRYLKQLPLLGSGKPDFVTLK 708
PLN03051 PLN03051
acyl-activating enzyme; Provisional
180-525 4.75e-12

acyl-activating enzyme; Provisional


Pssm-ID: 215552 [Multi-domain]  Cd Length: 499  Bit Score: 68.30  E-value: 4.75e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 180 AILsYTSGTTGNPKAV--TH-------CHGWgfAHLQMAPKHWLCineddlvWATAApgwqKWVWSPFL--SVLGSGATA 248
Cdd:PLN03051 123 NIL-FSSGTTGEPKAIpwTHlsplrcaSDGW--AHMDIQPGDVVC-------WPTNL----GWMMGPWLlySAFLNGATL 188
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 249 FVFNGrfSPET--YLELLQKYQINVLCCTPTEYRMMAK--LQNLNDYDLTHLHSAVSAGEPLNREvveqfkKYFNLTVRD 324
Cdd:PLN03051 189 ALYGG--APLGrgFGKFVQDAGVTVLGLVPSIVKAWRHtgAFAMEGLDWSKLRVFASTGEASAVD------DVLWLSSVR 260
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 325 GY--------GQTEstLLIGFLKDT---PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPAL--------- 384
Cdd:PLN03051 261 GYykpvieycGGTE--LASGYISSTllqPQAPGAFSTASLGTRFVLLNDNGVPYPDDQPCVGEVALAPPMLgasdrllna 338
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 385 --FKGYYK-EPERTAKAQTGKYYitGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTN-HPAVKECAV--VAK 458
Cdd:PLN03051 339 dhDKVYYKgMPMYGSKGMPLRRH--GDIMKRTPGGYFCVQGRADDTMNLGGIKTSSVEIERACDRaVAGIAETAAvgVAP 416
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 612673656 459 PH---DIRGNIVKAFVILQDHTAGD-DTLVKELQQFVKNEVAP-YKYPReIEFVDDLPKTNSGKIRRVELRD 525
Cdd:PLN03051 417 PDggpELLVIFLVLGEEKKGFDQARpEALQKKFQEAIQTNLNPlFKVSR-VKIVPELPRNASNKLLRRVLRD 487
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
50-416 7.40e-12

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 67.63  E-value: 7.40e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  50 TYQQLIEQSNKVGNVLASHGL--SKGDKVLIMMPRS----IATYELYIAALklgvAIIPCSEMLRTKDLQYRITHGEINA 123
Cdd:cd05927    7 SYKEVAERADNIGSALRSLGGkpAPASFVGIYSINRpewiISELACYAYSL----VTVPLYDTLGPEAIEYILNHAEISI 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 124 VIALEPFTVefekikeydaltkfviagqkdgwISL-EAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGwG 202
Cdd:cd05927   83 VFCDAGVKV-----------------------YSLeEFEKLGKKNKVPPPPPKPEDLATICYTSGTTGNPKGVMLTHG-N 138
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 203 FAHLQMA----PKHWLCINEDDlvwataapgwqkwVWSPFL------------SVLGSGATAFVFNGrfSPETYLELLQK 266
Cdd:cd05927  139 IVSNVAGvfkiLEILNKINPTD-------------VYISYLplahifervveaLFLYHGAKIGFYSG--DIRLLLDDIKA 203
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 267 YQINVLCCTPTEY-RMMAKLQN------------LN---DYDLTHLHSA---------------------------VSAG 303
Cdd:cd05927  204 LKPTVFPGVPRVLnRIYDKIFNkvqakgplkrklFNfalNYKLAELRSGvvraspfwdklvfnkikqalggnvrlmLTGS 283
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 304 EPLNREVVEQFKKYFNLTVRDGYGQTESTlLIGFLKDtPQ--RIGSMGKGIPGSSVTVVDdegnsVP--------ANTKG 373
Cdd:cd05927  284 APLSPEVLEFLRVALGCPVLEGYGQTECT-AGATLTL-PGdtSVGHVGGPLPCAEVKLVD-----VPemnydakdPNPRG 356
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....*
gi 612673656 374 NIAVplDLPALFKGYYKEPERTAKA--QTGkYYITGDLAHVDDDG 416
Cdd:cd05927  357 EVCI--RGPNVFSGYYKDPEKTAEAldEDG-WLHTGDIGEWLPNG 398
PRK05691 PRK05691
peptide synthase; Validated
30-524 2.59e-11

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 66.73  E-value: 2.59e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656   30 HIPDKKAILYHNTEGEDISV-TYQQLIEQSNKVGNVLASHGlSKGDKVLIMMPRSIATYELYIAALKLGVAIIPC--SEM 106
Cdd:PRK05691   21 QTPDRLALRFLADDPGEGVVlSYRDLDLRARTIAAALQARA-SFGDRAVLLFPSGPDYVAAFFGCLYAGVIAVPAypPES 99
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  107 LRTKDlQYRIThgeinAVIA-LEP---FTVE--FEKIKEYDALTkfviAGQKDGWISLEAEKENASNKLEMADTTRDDMA 180
Cdd:PRK05691  100 ARRHH-QERLL-----SIIAdAEPrllLTVAdlRDSLLQMEELA----AANAPELLCVDTLDPALAEAWQEPALQPDDIA 169
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  181 ILSYTSGTTGNPKAVTHCHGWGFAHLQMApKHWLCI--NEDDLVWAtaapgwqkwvWSPFLSVLG----------SGATA 248
Cdd:PRK05691  170 FLQYTSGSTALPKGVQVSHGNLVANEQLI-RHGFGIdlNPDDVIVS----------WLPLYHDMGliggllqpifSGVPC 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  249 FVFNGRF---SPETYLELLQKYQINVLCCTPTEYRMMAKL---QNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTV 322
Cdd:PRK05691  239 VLMSPAYfleRPLRWLEAISEYGGTISGGPDFAYRLCSERvseSALERLDLSRWRVAYSGSEPIRQDSLERFAEKFAACG 318
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  323 RD------GYGQTESTLL---------IGFLK-------------DTPQRIGSMGKGIPGSSVTVVD-DEGNSVPANTKG 373
Cdd:PRK05691  319 FDpdsffaSYGLAEATLFvsggrrgqgIPALEldaealarnraepGTGSVLMSCGRSQPGHAVLIVDpQSLEVLGDNRVG 398
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  374 NIAVplDLPALFKGYYKEPERTAKA---QTGKYYI-TGDLAHVDDdGYFWFEGRRDDIIISSGYTIGPFEVEDALTNhpa 449
Cdd:PRK05691  399 EIWA--SGPSIAHGYWRNPEASAKTfveHDGRTWLrTGDLGFLRD-GELFVTGRLKDMLIVRGHNLYPQDIEKTVER--- 472
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  450 vkECAVVAKPHdirgniVKAFVILQDHTAG----------------DDTLVKELQQFVKNevAPYKYPREIEFVDD--LP 511
Cdd:PRK05691  473 --EVEVVRKGR------VAAFAVNHQGEEGigiaaeisrsvqkilpPQALIKSIRQAVAE--ACQEAPSVVLLLNPgaLP 542
                         570
                  ....*....|...
gi 612673656  512 KTNSGKIRRVELR 524
Cdd:PRK05691  543 KTSSGKLQRSACR 555
LC_FACS_bac1 cd17641
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ...
170-456 2.99e-11

bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341296 [Multi-domain]  Cd Length: 569  Bit Score: 65.91  E-value: 2.99e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 170 EMADTTRDDMAILSYTSGTTGNPKAVTHCHG----WGFAHLQMAPKHwlciNEDDLVWATAAPgwqkWVWSPFLSVLGSG 245
Cdd:cd17641  151 EVAAGKGEDVAVLCTTSGTTGKPKLAMLSHGnflgHCAAYLAADPLG----PGDEYVSVLPLP----WIGEQMYSVGQAL 222
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 246 ATAFVFNGRFSPETYLELLQKYQINVLCCTPTEY---------RMM---------------AKLQNLND----------- 290
Cdd:cd17641  223 VCGFIVNFPEEPETMMEDLREIGPTFVLLPPRVWegiaadvraRMMdatpfkrfmfelgmkLGLRALDRgkrgrpvslwl 302
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 291 ------------------YDLTHLHSAVSAGEPLNREVveqFKKYFNLTV--RDGYGQTESTLLIGFLKDTPQRIGSMGK 350
Cdd:cd17641  303 rlaswladallfrplrdrLGFSRLRSAATGGAALGPDT---FRFFHAIGVplKQLYGQTELAGAYTVHRDGDVDPDTVGV 379
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 351 GIPGSSVTVvddegnsvpANTkGNIAVplDLPALFKGYYKEPERTAKAQTGKYYI-TGDLAHVDDDGYFWFEGRRDDI-I 428
Cdd:cd17641  380 PFPGTEVRI---------DEV-GEILV--RSPGVFVGYYKNPEATAEDFDEDGWLhTGDAGYFKENGHLVVIDRAKDVgT 447
                        330       340
                 ....*....|....*....|....*...
gi 612673656 429 ISSGYTIGPFEVEDALTNHPAVKEcAVV 456
Cdd:cd17641  448 TSDGTRFSPQFIENKLKFSPYIAE-AVV 474
PaaK COG1541
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ...
185-522 4.60e-09

Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];


Pssm-ID: 441150 [Multi-domain]  Cd Length: 423  Bit Score: 58.62  E-value: 4.60e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 185 TSGTTGNPKAVTHCH------GWGFAH-LQMAPkhwlcINEDDLVWATAAPGWqkwvWSPFLSV-LG---SGATAFVFNG 253
Cdd:COG1541   91 SSGTTGKPTVVGYTRkdldrwAELFARsLRAAG-----VRPGDRVQNAFGYGL----FTGGLGLhYGaerLGATVIPAGG 161
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 254 rFSPETYLELLQKYQINVLCCTPTeY--RMMAKLQNLN-DYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTE 330
Cdd:COG1541  162 -GNTERQLRLMQDFGPTVLVGTPS-YllYLAEVAEEEGiDPRDLSLKKGIFGGEPWSEEMRKEIEERWGIKAYDIYGLTE 239
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 331 STLLIGFlkDTPQRigsmgKG--IPGSS--VTVVDDE-GNSVPANTKGNI--------AVPLdLpalfkgyykepeRtak 397
Cdd:COG1541  240 VGPGVAY--ECEAQ-----DGlhIWEDHflVEIIDPEtGEPVPEGEEGELvvttltkeAMPL-I------------R--- 296
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 398 aqtgkyYITGDLAHVDDD------GYFWFE---GRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVK 468
Cdd:COG1541  297 ------YRTGDLTRLLPEpcpcgrTHPRIGrilGRADDMLIIRGVNVFPSQIEEVLLRIPEVGPEYQIVVDREGGLDELT 370
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 612673656 469 AFVILqDHTAGDDTLVKELQQFVKNEVapyKYPREIEFV--DDLPKTNsGKIRRVE 522
Cdd:COG1541  371 VRVEL-APGASLEALAEAIAAALKAVL---GLRAEVELVepGSLPRSE-GKAKRVI 421
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
173-460 1.11e-08

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 57.61  E-value: 1.11e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 173 DTTRDDMAILSYTSGTTGNPKAVTHCHG------WGFAHlqMAPKHwlcINEDDLVWAtaapgwqkwvWSPFLSVLGSGA 246
Cdd:cd17639   84 DGKPDDLACIMYTSGSTGNPKGVMLTHGnlvagiAGLGD--RVPEL---LGPDDRYLA----------YLPLAHIFELAA 148
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 247 TAFVF--NGRF---SPETYLEL--------LQKYQINVLCCTP----TEYR-MMAKLQNL-------------------- 288
Cdd:cd17639  149 ENVCLyrGGTIgygSPRTLTDKskrgckgdLTEFKPTLMVGVPaiwdTIRKgVLAKLNPMgglkrtlfwtayqsklkalk 228
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 289 NDYD--------------LT--HLHSAVSAGEPLNREVVEqFKKYFNLTVRDGYGQTEsTLLIGFLKDTPQ-RIGSMGKG 351
Cdd:cd17639  229 EGPGtplldelvfkkvraALggRLRYMLSGGAPLSADTQE-FLNIVLCPVIQGYGLTE-TCAGGTVQDPGDlETGRVGPP 306
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 352 IPGSSVTVVD-DEGNSVP--ANTKGNIAvpLDLPALFKGYYKEPERTAKAQTGK-YYITGDLAHVDDDGYFWFEGRRDDI 427
Cdd:cd17639  307 LPCCEIKLVDwEEGGYSTdkPPPRGEIL--IRGPNVFKGYYKNPEKTKEAFDGDgWFHTGDIGEFHPDGTLKIIDRKKDL 384
                        330       340       350
                 ....*....|....*....|....*....|....
gi 612673656 428 I-ISSGYTIGPFEVEDALTNHPAVKECAVVAKPH 460
Cdd:cd17639  385 VkLQNGEYIALEKLESIYRSNPLVNNICVYADPD 418
PLN02736 PLN02736
long-chain acyl-CoA synthetase
300-455 7.31e-08

long-chain acyl-CoA synthetase


Pssm-ID: 178337 [Multi-domain]  Cd Length: 651  Bit Score: 55.11  E-value: 7.31e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 300 VSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVD-------DEGNSVPantK 372
Cdd:PLN02736 382 SSGASPLSPDVMEFLRICFGGRVLEGYGMTETSCVISGMDEGDNLSGHVGSPNPACEVKLVDvpemnytSEDQPYP---R 458
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 373 GNIAVplDLPALFKGYYKEPERTAKAqtgkyyitgdlahVDDDGYF-------WFEGRRDDII--------ISSGYTIGP 437
Cdd:PLN02736 459 GEICV--RGPIIFKGYYKDEVQTREV-------------IDEDGWLhtgdiglWLPGGRLKIIdrkknifkLAQGEYIAP 523
                        170
                 ....*....|....*...
gi 612673656 438 FEVEDALTNHPAVKECAV 455
Cdd:PLN02736 524 EKIENVYAKCKFVAQCFV 541
PRK09029 PRK09029
O-succinylbenzoic acid--CoA ligase; Provisional
32-539 1.92e-07

O-succinylbenzoic acid--CoA ligase; Provisional


Pssm-ID: 236363 [Multi-domain]  Cd Length: 458  Bit Score: 53.72  E-value: 1.92e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  32 PDKKAILYHntegeDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKD 111
Cdd:PRK09029  17 PQAIALRLN-----DEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQPL 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 112 LQYRITHGEINAVIALEPFtvefekiKEYDALTkfviagqkdgwiSLEAEKENASNKLEMADttrDDMAILSYTSGTTGN 191
Cdd:PRK09029  92 LEELLPSLTLDFALVLEGE-------NTFSALT------------SLHLQLVEGAHAVAWQP---QRLATMTLTSGSTGL 149
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 192 PKAVTHCHGwgfAHLQMAPKhwLCinedDLVWATAAPGW-----------QK--WVWspflsvLGSGATaFVFNGrfsPE 258
Cdd:PRK09029 150 PKAAVHTAQ---AHLASAEG--VL----SLMPFTAQDSWllslplfhvsgQGivWRW------LYAGAT-LVVRD---KQ 210
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 259 TYLELLQKyqinvlcCT-----PTEyrmmakLQNLNDYDLTHL---H-----SAVSAgeplnrEVVEQFKKYfnlTVRD- 324
Cdd:PRK09029 211 PLEQALAG-------CThaslvPTQ------LWRLLDNRSEPLslkAvllggAAIPV------ELTEQAEQQ---GIRCw 268
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 325 -GYGQTE--STLligflkdTPQRI-GSMGKG--IPGSSVTVVDDE----GNSVpantkgniavpldlpALfkGYYKEPER 394
Cdd:PRK09029 269 cGYGLTEmaSTV-------CAKRAdGLAGVGspLPGREVKLVDGEiwlrGASL---------------AL--GYWRQGQL 324
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 395 TakaqtgkyyitgDLahVDDDGyfWFE---------------GRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKP 459
Cdd:PRK09029 325 V------------PL--VNDEG--WFAtrdrgewqngeltilGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVFVVPVA 388
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 460 HDIRGNIVKAFVILQDHTAgddtlVKELQQFVKNEVAPYKYPreIEFVdDLPKT--NSG-KIRRVELRDaeiekWQQQKD 536
Cdd:PRK09029 389 DAEFGQRPVAVVESDSEAA-----VVNLAEWLQDKLARFQQP--VAYY-LLPPElkNGGiKISRQALKE-----WVAQQL 455

                 ...
gi 612673656 537 SNQ 539
Cdd:PRK09029 456 GNN 458
PRK06814 PRK06814
acyl-[ACP]--phospholipid O-acyltransferase;
177-524 2.08e-07

acyl-[ACP]--phospholipid O-acyltransferase;


Pssm-ID: 235865 [Multi-domain]  Cd Length: 1140  Bit Score: 53.82  E-value: 2.08e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  177 DDMAILSYTSGTTGNPKAVTHCHGWGFAHL-QMAPKhwLCINEDDLVWaTAAPgwqkwVWSPFlsVLGSGATAFVFNG-- 253
Cdd:PRK06814  793 DDPAVILFTSGSEGTPKGVVLSHRNLLANRaQVAAR--IDFSPEDKVF-NALP-----VFHSF--GLTGGLVLPLLSGvk 862
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  254 RF---SPETYL---ELLqkYQIN--VLCCTPT---EYRMMAklqnlNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTV 322
Cdd:PRK06814  863 VFlypSPLHYRiipELI--YDTNatILFGTDTflnGYARYA-----HPYDFRSLRYVFAGAEKVKEETRQTWMEKFGIRI 935
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  323 RDGYGQTESTLLIGFlkDTPQ--RIGSMGKGIPG-----SSVTVVDDEGN-SVpantKGniavpldlPALFKGYYK-EPE 393
Cdd:PRK06814  936 LEGYGVTETAPVIAL--NTPMhnKAGTVGRLLPGieyrlEPVPGIDEGGRlFV----RG--------PNVMLGYLRaENP 1001
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  394 RTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFvil 473
Cdd:PRK06814 1002 GVLEPPADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAELWPDALHAAVSIPDARKGERIILL--- 1078
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|..
gi 612673656  474 qdhTAGDDTLVKELQQFVKNEVAPYKY-PREIEFVDDLPKTNSGKIRRVELR 524
Cdd:PRK06814 1079 ---TTASDATRAAFLAHAKAAGASELMvPAEIITIDEIPLLGTGKIDYVAVT 1127
PRK05850 PRK05850
acyl-CoA synthetase; Validated
26-523 2.91e-07

acyl-CoA synthetase; Validated


Pssm-ID: 235624 [Multi-domain]  Cd Length: 578  Bit Score: 53.02  E-value: 2.91e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  26 EKYSHIPDKKAILY----HNTEGEDISVTYQQLIEQSNKVGNVLASHGlSKGDKVLIMMPRSIATYELYIAALKLGVAII 101
Cdd:PRK05850   9 ERASLQPDDAAFTFidyeQDPAGVAETLTWSQLYRRTLNVAEELRRHG-STGDRAVILAPQGLEYIVAFLGALQAGLIAV 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 102 PCSEMlrtkdlQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDG---WISLEAEKENASNKLEMADTTRDD 178
Cdd:PRK05850  88 PLSVP------QGGAHDERVSAVLRDTSPSVVLTTSAVVDDVTEYVAPQPGQSappVIEVDLLDLDSPRGSDARPRDLPS 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 179 MAILSYTSGTTGNPKAVTHCHGWGFAHLQ------MAPKHwlcineddlvwATAAPGWQKWVWSPFLSVLG--SGATAFV 250
Cdd:PRK05850 162 TAYLQYTSGSTRTPAGVMVSHRNVIANFEqlmsdyFGDTG-----------GVPPPDTTVVSWLPFYHDMGlvLGVCAPI 230
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 251 FNGR----FSPETYlelLQKyqinvlcctPTEY-RMMAKLQ---------------------NLNDYDLTHLHSAVSAGE 304
Cdd:PRK05850 231 LGGCpavlTSPVAF---LQR---------PARWmQLLASNPhafsaapnfafelavrktsddDMAGLDLGGVLGIISGSE 298
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 305 PLN----REVVEQFKKyFNL---TVRDGYGQTESTLLI---------------------GFLKDTPQRIGS--MGKGIPG 354
Cdd:PRK05850 299 RVHpatlKRFADRFAP-FNLretAIRPSYGLAEATVYVatrepgqppesvrfdyeklsaGHAKRCETGGGTplVSYGSPR 377
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 355 SS-VTVVDDEGN-SVPANTKGNIAVPLDLPALfkGYYKEP---ERTAKAQ---------TGKYYITGDLAHVDDDGYFwF 420
Cdd:PRK05850 378 SPtVRIVDPDTCiECPAGTVGEIWVHGDNVAA--GYWQKPeetERTFGATlvdpspgtpEGPWLRTGDLGFISEGELF-I 454
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 421 EGRRDDIIISSGYTIGPFEVEdaltnhPAVKE-----CAVVAKPHDIRGNIVkafVILQDHTAGD-DTLVKELQQFVKNE 494
Cdd:PRK05850 455 VGRIKDLLIVDGRNHYPDDIE------ATIQEitggrVAAISVPDDGTEKLV---AIIELKKRGDsDEEAMDRLRTVKRE 525
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|..
gi 612673656 495 V--APYKYPREIefVDDL--------PKTNSGKIRR---VEL 523
Cdd:PRK05850 526 VtsAISKSHGLS--VADLvlvapgsiPITTSGKIRRaacVEQ 565
PTZ00216 PTZ00216
acyl-CoA synthetase; Provisional
174-416 3.93e-06

acyl-CoA synthetase; Provisional


Pssm-ID: 240316 [Multi-domain]  Cd Length: 700  Bit Score: 49.59  E-value: 3.93e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 174 TTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLqMAPKHWL-----CINEDDLVWAtaapgwqkwvWSPFLSVLGSGATA 248
Cdd:PTZ00216 261 ENNDDLALIMYTSGTTGDPKGVMHTHGSLTAGI-LALEDRLndligPPEEDETYCS----------YLPLAHIMEFGVTN 329
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 249 -FVFNGRF----SPETYLEL-------LQKYQINVLCCTPTEYRMMAK------------------------LQNLNDYD 292
Cdd:PTZ00216 330 iFLARGALigfgSPRTLTDTfarphgdLTEFRPVFLIGVPRIFDTIKKaveaklppvgslkrrvfdhayqsrLRALKEGK 409
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 293 LT-----------------HLHSAVSAGEPLNREVVEQFKKYFNLTVRdGYGQTEsTLLIGflkdTPQRIGSM-----GK 350
Cdd:PTZ00216 410 DTpywnekvfsapravlggRVRAMLSGGGPLSAATQEFVNVVFGMVIQ-GWGLTE-TVCCG----GIQRTGDLepnavGQ 483
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 351 GIPGSSVTVVDDEG---NSVPaNTKGNIAvpLDLPALFKGYYKEPERTAKAQT-GKYYITGDLAHVDDDG 416
Cdd:PTZ00216 484 LLKGVEMKLLDTEEykhTDTP-EPRGEIL--LRGPFLFKGYYKQEELTREVLDeDGWFHTGDVGSIAANG 550
PLN02614 PLN02614
long-chain acyl-CoA synthetase
25-472 6.02e-05

long-chain acyl-CoA synthetase


Pssm-ID: 166255 [Multi-domain]  Cd Length: 666  Bit Score: 45.78  E-value: 6.02e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656  25 IEKYSHIP--DKKAILyHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIM---MPRSIATYElyiAALKLGVA 99
Cdd:PLN02614  55 VEKYPNNPmlGRREIV-DGKPGKYVWQTYQEVYDIVIKLGNSLRSVGVKDEAKCGIYganSPEWIISME---ACNAHGLY 130
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 100 IIPCSEMLRTKDLQYRITHGEINAVIalepftVEFEKIKEYDAL----TKFVIAGQKDGWISLEaEKENASN-------- 167
Cdd:PLN02614 131 CVPLYDTLGAGAVEFIISHSEVSIVF------VEEKKISELFKTcpnsTEYMKTVVSFGGVSRE-QKEEAETfglviyaw 203
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 168 ----------KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHG------WGFAHLQMAPKHWLCINEDDLVWATAAPGWQ 231
Cdd:PLN02614 204 deflklgegkQYDLPIKKKSDICTIMYTSGTTGDPKGVMISNEsivtliAGVIRLLKSANAALTVKDVYLSYLPLAHIFD 283
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 232 KWVWSPFLSVlgSGATAFvfnGRFSPETYLELLQKYQINVLCCTPTEY-RMMAKLQN-LND--------------YDLTH 295
Cdd:PLN02614 284 RVIEECFIQH--GAAIGF---WRGDVKLLIEDLGELKPTIFCAVPRVLdRVYSGLQKkLSDggflkkfvfdsafsYKFGN 358
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 296 L---HSAVSAGEPLNREVVEQFKKYFNLTVR--------------------------DGYGQTESTllIGFLKDTPQRIG 346
Cdd:PLN02614 359 MkkgQSHVEASPLCDKLVFNKVKQGLGGNVRiilsgaaplashvesflrvvacchvlQGYGLTESC--AGTFVSLPDELD 436
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 347 SMGKgiPGSSVTVVDDEGNSVP--------ANTKGNIAVplDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYF 418
Cdd:PLN02614 437 MLGT--VGPPVPNVDIRLESVPemeydalaSTPRGEICI--RGKTLFSGYYKREDLTKEVLIDGWLHTGDVGEWQPNGSM 512
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 612673656 419 WFEGRRDDII-ISSGYTIGPFEVEDALTNHPAVKECAVVakphdirGNIVKAFVI 472
Cdd:PLN02614 513 KIIDRKKNIFkLSQGEYVAVENIENIYGEVQAVDSVWVY-------GNSFESFLV 560
PTZ00342 PTZ00342
acyl-CoA synthetase; Provisional
300-423 7.05e-05

acyl-CoA synthetase; Provisional


Pssm-ID: 240370 [Multi-domain]  Cd Length: 746  Bit Score: 45.48  E-value: 7.05e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 300 VSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIgFLKD-TPQRIGSMGKGI-PGSSVTVVDDE----GNSVPantKG 373
Cdd:PTZ00342 467 LNGGGKLSPKIAEELSVLLNVNYYQGYGLTETTGPI-FVQHaDDNNTESIGGPIsPNTKYKVRTWEtykaTDTLP---KG 542
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 612673656 374 NIAVPLDlpALFKGYYKEPERTAKAQT-GKYYITGDLAHVDDDGYFWFEGR 423
Cdd:PTZ00342 543 ELLIKSD--SIFSGYFLEKEQTKNAFTeDGYFKTGDIVQINKNGSLTFLDR 591
PaaK cd05913
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ...
256-451 8.33e-05

Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.


Pssm-ID: 341239 [Multi-domain]  Cd Length: 425  Bit Score: 44.92  E-value: 8.33e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 256 SPETYLELLQKYQINVLCCTPTEYRMMA-KLQNLN-DYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTEstl 333
Cdd:cd05913  158 NTERQLQLIKDFGPTVLCCTPSYALYLAeEAEEEGiDPRELSLKVGIFGAEPWTEEMRKRIERRLGIKAYDIYGLTE--- 234
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 334 ligflkdtpqrIGSMGKGIPGSS------------VTVVDDE-GNSVPANTKGNI--------AVPL------DLPALfk 386
Cdd:cd05913  235 -----------IIGPGVAFECEEkdglhiwedhfiPEIIDPEtGEPVPPGEVGELvfttltkeAMPLiryrtrDITRL-- 301
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 612673656 387 gyykEPERTAKAQTgkyyitgdlaHVDDDGyfwFEGRRDDIIISSGYTIGPFEVEDALTNHPAVK 451
Cdd:cd05913  302 ----LPGPCPCGRT----------HRRIDR---ITGRSDDMLIIRGVNVFPSQIEDVLLKIPGLG 349
PLN02430 PLN02430
long-chain-fatty-acid-CoA ligase
300-452 7.65e-04

long-chain-fatty-acid-CoA ligase


Pssm-ID: 178049 [Multi-domain]  Cd Length: 660  Bit Score: 42.11  E-value: 7.65e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 300 VSAGEPLNREVVEQFKKYFNLTVRDGYGQTEST--LLIGFlkdtPQRIGSMGK-GIPGSSVTV----VDDEG-NSVPANT 371
Cdd:PLN02430 389 ISGGAPLSTEIEEFLRVTSCAFVVQGYGLTETLgpTTLGF----PDEMCMLGTvGAPAVYNELrleeVPEMGyDPLGEPP 464
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 612673656 372 KGNIAVPLDlpALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDII-ISSGYTIGPFEVEDALTNHPAV 450
Cdd:PLN02430 465 RGEICVRGK--CLFSGYYKNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIkLSQGEYVALEYLENVYGQNPIV 542

                 ..
gi 612673656 451 KE 452
Cdd:PLN02430 543 ED 544
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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