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Conserved domains on  [gi|119628717|gb|EAX08312|]
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EF-hand domain family, member A1, isoform CRA_a, partial [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EFh_MICU super family cl28896
EF-hand, calcium binding motif, found in mitochondrial calcium uptake proteins MICU1, MICU2, ...
 176-218 3.64e-16

EF-hand, calcium binding motif, found in mitochondrial calcium uptake proteins MICU1, MICU2, MICU3, and similar proteins; This family includes mitochondrial calcium uptake protein MICU1 and its two additional paralogs, MICU2 and MICU3. MICU1 localizes to the inner mitochondrial membrane (IMM). It functions as a gatekeeper of the mitochondrial calcium uniporter (MCU) and regulates MCU-mediated mitochondrial Ca2+ uptake, which is essential for maintaining mitochondrial homoeostasis. MICU1 and MICU2 are physically associated within the uniporter complex and are co-expressed across all tissues. They may play non-redundant roles in the regulation of the mitochondrial calcium uniporter. At present, the precise molecular function of MICU2 and MICU3 remain unclear. MICU2 may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU3 likely has a role in mitochondrial calcium handling. All members in this family contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


The actual alignment was detected with superfamily member cd16174:

Pssm-ID: 333716 [Multi-domain]  Cd Length: 154  Bit Score: 72.60  E-value: 3.64e-16
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 119628717 176 GFHVAFKMLDTDGNEMIEKREFFK---------------------EAIVKEPEINTTLQMRFFG 218
Cdd:cd16174    1 GFHIAFKMLDTDGNEQVEKREFFKlqkiigkkddlmtqggtetyqEASDNSDEVNTTLQVHFFG 64
FRQ1 super family cl34916
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
151-199 4.44e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


The actual alignment was detected with superfamily member COG5126:

Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 36.31  E-value: 4.44e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 119628717 151 DLGDKGLISYTEYLFLLTILTKPHSGFHVAFKMLDTDGNEMIEKREFFK 199
Cdd:COG5126   79 DTDGDGKISADEFRRLLTALGVSEEEADELFARLDTDGDGKISFEEFVA 127
 
Name Accession Description Interval E-value
EFh_MICU2 cd16174
EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and ...
 176-218 3.64e-16

EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and similar proteins; MICU2, also termed EF-hand domain-containing family member A1 (EFHA1), is a mitochondrial-localized paralog of MICU1. MICU2 and its paralog, MICU1, are physically associated within the mitochondrial calcium uniporter (MCU) complex and are co-expressed across all tissues. They may operate together with MCU to regulate the channel. At present, the precise molecular function of MICU2 remains unclear. It may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU2 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320082 [Multi-domain]  Cd Length: 154  Bit Score: 72.60  E-value: 3.64e-16
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 119628717 176 GFHVAFKMLDTDGNEMIEKREFFK---------------------EAIVKEPEINTTLQMRFFG 218
Cdd:cd16174    1 GFHIAFKMLDTDGNEQVEKREFFKlqkiigkkddlmtqggtetyqEASDNSDEVNTTLQVHFFG 64
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
151-199 4.44e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 36.31  E-value: 4.44e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 119628717 151 DLGDKGLISYTEYLFLLTILTKPHSGFHVAFKMLDTDGNEMIEKREFFK 199
Cdd:COG5126   79 DTDGDGKISADEFRRLLTALGVSEEEADELFARLDTDGDGKISFEEFVA 127
 
Name Accession Description Interval E-value
EFh_MICU2 cd16174
EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and ...
 176-218 3.64e-16

EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and similar proteins; MICU2, also termed EF-hand domain-containing family member A1 (EFHA1), is a mitochondrial-localized paralog of MICU1. MICU2 and its paralog, MICU1, are physically associated within the mitochondrial calcium uniporter (MCU) complex and are co-expressed across all tissues. They may operate together with MCU to regulate the channel. At present, the precise molecular function of MICU2 remains unclear. It may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU2 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320082 [Multi-domain]  Cd Length: 154  Bit Score: 72.60  E-value: 3.64e-16
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 119628717 176 GFHVAFKMLDTDGNEMIEKREFFK---------------------EAIVKEPEINTTLQMRFFG 218
Cdd:cd16174    1 GFHIAFKMLDTDGNEQVEKREFFKlqkiigkkddlmtqggtetyqEASDNSDEVNTTLQVHFFG 64
EFh_MICU cd15900
EF-hand, calcium binding motif, found in mitochondrial calcium uptake proteins MICU1, MICU2, ...
 176-218 1.07e-08

EF-hand, calcium binding motif, found in mitochondrial calcium uptake proteins MICU1, MICU2, MICU3, and similar proteins; This family includes mitochondrial calcium uptake protein MICU1 and its two additional paralogs, MICU2 and MICU3. MICU1 localizes to the inner mitochondrial membrane (IMM). It functions as a gatekeeper of the mitochondrial calcium uniporter (MCU) and regulates MCU-mediated mitochondrial Ca2+ uptake, which is essential for maintaining mitochondrial homoeostasis. MICU1 and MICU2 are physically associated within the uniporter complex and are co-expressed across all tissues. They may play non-redundant roles in the regulation of the mitochondrial calcium uniporter. At present, the precise molecular function of MICU2 and MICU3 remain unclear. MICU2 may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU3 likely has a role in mitochondrial calcium handling. All members in this family contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320080 [Multi-domain]  Cd Length: 152  Bit Score: 52.23  E-value: 1.07e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 119628717 176 GFHVAFKMLDTDGNEMIEKREFFK-------------------EAIVKEPEINTTLQMRFFG 218
Cdd:cd15900    1 HFEIAFKMFDLDGDGELDKEEFNKvqsiirsqtsvgqrhrdhtNGESTKLGMNSTLARYFFG 62
EFh_MICU3 cd16175
EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and ...
 176-218 3.57e-06

EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and similar proteins; MICU3, also termed EF-hand domain-containing family member A2 (EFHA2), is a paralog of MICU1 and notably found in the central nervous system (CNS) and skeletal muscle. At present, the precise molecular function of MICU3 remains unclear. It likely has a role in mitochondrial calcium handling. MICU3 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320083 [Multi-domain]  Cd Length: 128  Bit Score: 44.81  E-value: 3.57e-06
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 119628717 176 GFHVAFKMLDTDGNEMIEKREFfkeaIVKEpEINTTLQMRFFG 218
Cdd:cd16175    1 GFRIAFNMFDTDGNEMVDKKEF----LVLQ-EIFRTLLVHFFG 38
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
151-199 4.44e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 36.31  E-value: 4.44e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 119628717 151 DLGDKGLISYTEYLFLLTILTKPHSGFHVAFKMLDTDGNEMIEKREFFK 199
Cdd:COG5126   79 DTDGDGKISADEFRRLLTALGVSEEEADELFARLDTDGDGKISFEEFVA 127
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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