NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|119575122|gb|EAW54735|]
View 

hCG1987937, isoform CRA_a [Homo sapiens]

Protein Classification

nuclear transport factor 2 family protein( domain architecture ID 233)

nuclear transport factor 2 (NTF2) family protein, similar to Aspergillus flavus scytalone dehydratases, Pseudomonas putida benzene 1,2-dioxygenase subunit beta, and many other members with similar structural details but divergent functions

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
NTF2_like super family cl09109
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 215-251 2.16e-05

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


The actual alignment was detected with superfamily member cd00780:

Pssm-ID: 471850  Cd Length: 119  Bit Score: 43.04  E-value: 2.16e-05
                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 119575122 215 TIKSLVLQFLLQYYLIYDsEDRTGLLSVYHDKACFSL 251
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFD-NNREGLHRLYGDTSMLSR 36
LRR super family cl34836
Leucine-rich repeat (LRR) protein [Transcription];
96-160 7.40e-04

Leucine-rich repeat (LRR) protein [Transcription];


The actual alignment was detected with superfamily member COG4886:

Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 41.07  E-value: 7.40e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 119575122  96 NFPELLSLNLCDNKLyhlDGLPDIIEKAPKVKTLNLSKNKLKS-AWELGKVKglKLEELWLEGNSL 160
Cdd:COG4886  157 NLTNLKSLDLSNNQL---TDLPEELGNLTNLKELDLSNNQITDlPEPLGNLT--NLEELDLSGNQL 217
 
Name Accession Description Interval E-value
NTF2 cd00780
Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of ...
 215-251 2.16e-05

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.


Pssm-ID: 238403  Cd Length: 119  Bit Score: 43.04  E-value: 2.16e-05
                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 119575122 215 TIKSLVLQFLLQYYLIYDsEDRTGLLSVYHDKACFSL 251
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFD-NNREGLHRLYGDTSMLSR 36
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
96-160 7.40e-04

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 41.07  E-value: 7.40e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 119575122  96 NFPELLSLNLCDNKLyhlDGLPDIIEKAPKVKTLNLSKNKLKS-AWELGKVKglKLEELWLEGNSL 160
Cdd:COG4886  157 NLTNLKSLDLSNNQL---TDLPEELGNLTNLKELDLSNNQITDlPEPLGNLT--NLEELDLSGNQL 217
 
Name Accession Description Interval E-value
NTF2 cd00780
Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of ...
 215-251 2.16e-05

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.


Pssm-ID: 238403  Cd Length: 119  Bit Score: 43.04  E-value: 2.16e-05
                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 119575122 215 TIKSLVLQFLLQYYLIYDsEDRTGLLSVYHDKACFSL 251
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFD-NNREGLHRLYGDTSMLSR 36
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
96-160 7.40e-04

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 41.07  E-value: 7.40e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 119575122  96 NFPELLSLNLCDNKLyhlDGLPDIIEKAPKVKTLNLSKNKLKS-AWELGKVKglKLEELWLEGNSL 160
Cdd:COG4886  157 NLTNLKSLDLSNNQL---TDLPEELGNLTNLKELDLSNNQITDlPEPLGNLT--NLEELDLSGNQL 217
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
96-160 7.60e-04

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 41.07  E-value: 7.60e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 119575122  96 NFPELLSLNLCDNKLYHLdglPDIIEKAPKVKTLNLSKNKLKSAWELGKVKglKLEELWLEGNSL 160
Cdd:COG4886  203 NLTNLEELDLSGNQLTDL---PEPLANLTNLETLDLSNNQLTDLPELGNLT--NLEELDLSNNQL 262
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
96-160 1.81e-03

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 39.92  E-value: 1.81e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 119575122  96 NFPELLSLNLCDNKLyhlDGLPDIIEKAPKVKTLNLSKNKLKSAWE-LGKVKglKLEELWLEGNSL 160
Cdd:COG4886  111 NLTNLESLDLSGNQL---TDLPEELANLTNLKELDLSNNQLTDLPEpLGNLT--NLKSLDLSNNQL 171
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
96-160 4.40e-03

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 38.76  E-value: 4.40e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 119575122  96 NFPELLSLNLCDNKLyhlDGLPDIIEKAPKVKTLNLSKNKLKSA-WELGKVKglKLEELWLEGNSL 160
Cdd:COG4886  180 NLTNLKELDLSNNQI---TDLPEPLGNLTNLEELDLSGNQLTDLpEPLANLT--NLETLDLSNNQL 240
NTF2_like cd00531
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 220-320 4.78e-03

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


Pssm-ID: 238296 [Multi-domain]  Cd Length: 124  Bit Score: 36.72  E-value: 4.78e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 119575122 220 VLQFLLQYYLIYDSEDRTGLLSVYHDKACFSLTITLNPEDPEP--SSLEKYFKdsrniknikdpclRIQLLKHTKREIVD 297
Cdd:cd00531    1 AEQFLYRYARLLDAGDREWLALLYADDAYFEPPGGDGLIYPDDgrEAIEDRVR-------------RLPFGPSRTRHLVS 67

                         90       100
                 ....*....|....*....|...
gi 119575122 298 SLSVLPRTQHDLNSYVVDLCIQT 320
Cdd:cd00531   68 NVDVQPGDDGEGVVVSVFGVLRT 90
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH