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Conserved domains on  [gi|524044635|emb|CCY26553|]
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modification methylase EcoRI (Adenine-specificmethyltransferase EcoRI) [Firmicutes bacterium CAG:114]

Protein Classification

adenine-specific methyltransferase EcoRI family protein( domain architecture ID 10614878)

adenine-specific methyltransferase EcoRI family protein similar to Escherichia coli EcoRI that recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EcoRI_methylase pfam13651
Adenine-specific methyltransferase EcoRI; This methylase recognizes the double-stranded ...
 7-372 0e+00

Adenine-specific methyltransferase EcoRI; This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.


:

Pssm-ID: 404530  Cd Length: 343  Bit Score: 565.46  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635    7 LNSAKTARKDEFYTQLTDIEKEMRYYRKHFKGKTVLCNCDDPFESNFFKYFVLNFNRLGLKKLIATCYSTSPIMGSHFhy 86
Cdd:pfam13651   1 LNKAKKAKKDEFYTQLSDIERELRHYPNHFKDKVVLCNCDDPRESNFFKYFALNFNYLGLKKLIATCYNGSPIAGQEL-- 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635   87 hvdgsgqisfsFEDEAVIDNDSKRPYKATVTQVYDTTGDGGVDMLDVAKLfmtgdnelvELNGDGDFRSPECIELLDEAD 166
Cdd:pfam13651  79 -----------QDFSLFSDEDKKKAYKLEYTGVKDTNGDGAIDLEDIQIL---------PLKGDGDFRSAECIELLKQAD 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635  167 IVVTNPPFSLFREYLGTLVEHQKHFIIIGNVNAVKYQEVFPLFMSNELWLGASIHSGDRAFFVPDDYPLDAAGCGVDgET 246
Cdd:pfam13651 139 IVVTNPPFSLFREYVAQLVEYDKKFLIIGNQNAITYKEIFPLIKENKLWLGYGFGSGDMGFIVPDDYELYATRCFVD-ED 217

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635  247 GRKYIRVKGVRWFTNLDIKQRHEEMILVRRYTPDEYPKYVNYDAIEVAKTSDIPCDYAGAMGVPITFMDKYNPDQFEIVG 326
Cdd:pfam13651 218 GQKYIRVKGVCWFTNLDIGKRHEDLVLYKRYNEEEYPKYDNYDAIEVNKTDDIPCDYDGIMGVPITFLDKYNPDQFEIIG 297

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*...
gi 524044635  327 ASQHLIREISDDIRAKARYSQIGcfYLDNGDGT--YRKMYDRIVIRNK 372
Cdd:pfam13651 298 LRAGQYAGAAGTGTNKYGLSKHG--ADRKKDPRvnGKRPYFRILIRNK 343
 
Name Accession Description Interval E-value
EcoRI_methylase pfam13651
Adenine-specific methyltransferase EcoRI; This methylase recognizes the double-stranded ...
 7-372 0e+00

Adenine-specific methyltransferase EcoRI; This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.


Pssm-ID: 404530  Cd Length: 343  Bit Score: 565.46  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635    7 LNSAKTARKDEFYTQLTDIEKEMRYYRKHFKGKTVLCNCDDPFESNFFKYFVLNFNRLGLKKLIATCYSTSPIMGSHFhy 86
Cdd:pfam13651   1 LNKAKKAKKDEFYTQLSDIERELRHYPNHFKDKVVLCNCDDPRESNFFKYFALNFNYLGLKKLIATCYNGSPIAGQEL-- 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635   87 hvdgsgqisfsFEDEAVIDNDSKRPYKATVTQVYDTTGDGGVDMLDVAKLfmtgdnelvELNGDGDFRSPECIELLDEAD 166
Cdd:pfam13651  79 -----------QDFSLFSDEDKKKAYKLEYTGVKDTNGDGAIDLEDIQIL---------PLKGDGDFRSAECIELLKQAD 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635  167 IVVTNPPFSLFREYLGTLVEHQKHFIIIGNVNAVKYQEVFPLFMSNELWLGASIHSGDRAFFVPDDYPLDAAGCGVDgET 246
Cdd:pfam13651 139 IVVTNPPFSLFREYVAQLVEYDKKFLIIGNQNAITYKEIFPLIKENKLWLGYGFGSGDMGFIVPDDYELYATRCFVD-ED 217

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635  247 GRKYIRVKGVRWFTNLDIKQRHEEMILVRRYTPDEYPKYVNYDAIEVAKTSDIPCDYAGAMGVPITFMDKYNPDQFEIVG 326
Cdd:pfam13651 218 GQKYIRVKGVCWFTNLDIGKRHEDLVLYKRYNEEEYPKYDNYDAIEVNKTDDIPCDYDGIMGVPITFLDKYNPDQFEIIG 297

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*...
gi 524044635  327 ASQHLIREISDDIRAKARYSQIGcfYLDNGDGT--YRKMYDRIVIRNK 372
Cdd:pfam13651 298 LRAGQYAGAAGTGTNKYGLSKHG--ADRKKDPRvnGKRPYFRILIRNK 343
 
Name Accession Description Interval E-value
EcoRI_methylase pfam13651
Adenine-specific methyltransferase EcoRI; This methylase recognizes the double-stranded ...
 7-372 0e+00

Adenine-specific methyltransferase EcoRI; This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.


Pssm-ID: 404530  Cd Length: 343  Bit Score: 565.46  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635    7 LNSAKTARKDEFYTQLTDIEKEMRYYRKHFKGKTVLCNCDDPFESNFFKYFVLNFNRLGLKKLIATCYSTSPIMGSHFhy 86
Cdd:pfam13651   1 LNKAKKAKKDEFYTQLSDIERELRHYPNHFKDKVVLCNCDDPRESNFFKYFALNFNYLGLKKLIATCYNGSPIAGQEL-- 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635   87 hvdgsgqisfsFEDEAVIDNDSKRPYKATVTQVYDTTGDGGVDMLDVAKLfmtgdnelvELNGDGDFRSPECIELLDEAD 166
Cdd:pfam13651  79 -----------QDFSLFSDEDKKKAYKLEYTGVKDTNGDGAIDLEDIQIL---------PLKGDGDFRSAECIELLKQAD 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635  167 IVVTNPPFSLFREYLGTLVEHQKHFIIIGNVNAVKYQEVFPLFMSNELWLGASIHSGDRAFFVPDDYPLDAAGCGVDgET 246
Cdd:pfam13651 139 IVVTNPPFSLFREYVAQLVEYDKKFLIIGNQNAITYKEIFPLIKENKLWLGYGFGSGDMGFIVPDDYELYATRCFVD-ED 217

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524044635  247 GRKYIRVKGVRWFTNLDIKQRHEEMILVRRYTPDEYPKYVNYDAIEVAKTSDIPCDYAGAMGVPITFMDKYNPDQFEIVG 326
Cdd:pfam13651 218 GQKYIRVKGVCWFTNLDIGKRHEDLVLYKRYNEEEYPKYDNYDAIEVNKTDDIPCDYDGIMGVPITFLDKYNPDQFEIIG 297

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*...
gi 524044635  327 ASQHLIREISDDIRAKARYSQIGcfYLDNGDGT--YRKMYDRIVIRNK 372
Cdd:pfam13651 298 LRAGQYAGAAGTGTNKYGLSKHG--ADRKKDPRvnGKRPYFRILIRNK 343
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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