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Conserved domains on  [gi|302142768|emb|CBI19971|]
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unnamed protein product, partial [Vitis vinifera]

Protein Classification

glycosyltransferase family protein( domain architecture ID 27718)

glycosyltransferase family protein may synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Glyco_tranf_GTA_type super family cl11394
Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a ...
 337-471 7.94e-30

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.


The actual alignment was detected with superfamily member cd02537:

Pssm-ID: 472172 [Multi-domain]  Cd Length: 240  Bit Score: 117.36  E-value: 7.94e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302142768 337 EISAIGNN--ATLFNSGVMVIEPSNCTFQLLMDHINEIESYNGGDQGYLNEIFTW---WHRIPKHMNFLKHFWEGDEEEK 411
Cdd:cd02537  115 EFAAAPDCgwPDLFNSGVFVLKPSEETFNDLLDALQDTPSFDGGDQGLLNSYFSDrgiWKRLPFTYNALKPLRYLHPEAL 194

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 302142768 412 kemktrlfgADPPVLYVLHYLG-LKPWLCFRDYDCNWNVDIlqefasNVAHKRWWKIHDAM 471
Cdd:cd02537  195 ---------WFGDEIKVVHFIGgDKPWSWWRDPETKEKDDY------NELHQWWWDIYDEL 240
 
Name Accession Description Interval E-value
GT8_Glycogenin cd02537
Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen; Glycogenin ...
 337-471 7.94e-30

Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen; Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases.


Pssm-ID: 133018 [Multi-domain]  Cd Length: 240  Bit Score: 117.36  E-value: 7.94e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302142768 337 EISAIGNN--ATLFNSGVMVIEPSNCTFQLLMDHINEIESYNGGDQGYLNEIFTW---WHRIPKHMNFLKHFWEGDEEEK 411
Cdd:cd02537  115 EFAAAPDCgwPDLFNSGVFVLKPSEETFNDLLDALQDTPSFDGGDQGLLNSYFSDrgiWKRLPFTYNALKPLRYLHPEAL 194

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 302142768 412 kemktrlfgADPPVLYVLHYLG-LKPWLCFRDYDCNWNVDIlqefasNVAHKRWWKIHDAM 471
Cdd:cd02537  195 ---------WFGDEIKVVHFIGgDKPWSWWRDPETKEKDDY------NELHQWWWDIYDEL 240
Gnt1 COG5597
N-acetylglucosaminyl transferase [Cell wall/membrane/envelope biogenesis];
349-437 2.02e-05

N-acetylglucosaminyl transferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 444333 [Multi-domain]  Cd Length: 279  Bit Score: 46.65  E-value: 2.02e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302142768 349 NSGVMVIEPSNCTFQLLMDHINEIESY-NGGDQGYLNEIFTWWHRIPKHMNFLKHFWEGDEEekkemktrLFgaDPPVLY 427
Cdd:COG5597  163 NSGVFTARPSQATFEAMLARLDAPGAFwRRTDQTFLQTFFPDWHGLPVFMNMLQYVWFNLPE--------LW--DWPSIR 232

                         90
                 ....*....|
gi 302142768 428 VLHYLGLKPW 437
Cdd:COG5597  233 VLHYQYEKPW 242
 
Name Accession Description Interval E-value
GT8_Glycogenin cd02537
Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen; Glycogenin ...
 337-471 7.94e-30

Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen; Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases.


Pssm-ID: 133018 [Multi-domain]  Cd Length: 240  Bit Score: 117.36  E-value: 7.94e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302142768 337 EISAIGNN--ATLFNSGVMVIEPSNCTFQLLMDHINEIESYNGGDQGYLNEIFTW---WHRIPKHMNFLKHFWEGDEEEK 411
Cdd:cd02537  115 EFAAAPDCgwPDLFNSGVFVLKPSEETFNDLLDALQDTPSFDGGDQGLLNSYFSDrgiWKRLPFTYNALKPLRYLHPEAL 194

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 302142768 412 kemktrlfgADPPVLYVLHYLG-LKPWLCFRDYDCNWNVDIlqefasNVAHKRWWKIHDAM 471
Cdd:cd02537  195 ---------WFGDEIKVVHFIGgDKPWSWWRDPETKEKDDY------NELHQWWWDIYDEL 240
Gnt1 COG5597
N-acetylglucosaminyl transferase [Cell wall/membrane/envelope biogenesis];
349-437 2.02e-05

N-acetylglucosaminyl transferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 444333 [Multi-domain]  Cd Length: 279  Bit Score: 46.65  E-value: 2.02e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302142768 349 NSGVMVIEPSNCTFQLLMDHINEIESY-NGGDQGYLNEIFTWWHRIPKHMNFLKHFWEGDEEekkemktrLFgaDPPVLY 427
Cdd:COG5597  163 NSGVFTARPSQATFEAMLARLDAPGAFwRRTDQTFLQTFFPDWHGLPVFMNMLQYVWFNLPE--------LW--DWPSIR 232

                         90
                 ....*....|
gi 302142768 428 VLHYLGLKPW 437
Cdd:COG5597  233 VLHYQYEKPW 242
RfaJ COG1442
Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope ...
 348-445 2.70e-05

Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441051 [Multi-domain]  Cd Length: 301  Bit Score: 46.12  E-value: 2.70e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302142768 348 FNSGVMVIEP----SNCTFQLLMDHINE-IESYNGGDQGYLNEIFT-WWHRIPKHMNFLkHFWEGDEEEKKEMKTRLFGA 421
Cdd:COG1442  158 FNSGVLLINLkkwrEENITEKALEFLKEnPDKLKYPDQDILNIVLGgKVKFLPPRYNYQ-YSLYYELKDKSNKKELLEAR 236

                         90       100
                 ....*....|....*....|....*
gi 302142768 422 DPPVlyVLHYLGL-KPWLCFRDYDC 445
Cdd:COG1442  237 KNPV--IIHYTGPtKPWHKWCTHPY 259
GT8_A4GalT_like cd04194
A4GalT_like proteins catalyze the addition of galactose or glucose residues to the ...
 348-437 8.87e-04

A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface; The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.


Pssm-ID: 133037 [Multi-domain]  Cd Length: 248  Bit Score: 41.05  E-value: 8.87e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302142768 348 FNSGVMVI------EpsNCTFQLLMDHINEiesYNG----GDQGYLNEIF-TWWHRIPKHMNFLK-HFWEGDEEEKKEMK 415
Cdd:cd04194  152 FNSGVLLInlkkwrE--ENITEKLLELIKE---YGGrliyPDQDILNAVLkDKILYLPPRYNFQTgFYYLLKKKSKEEQE 226

                         90       100
                 ....*....|....*....|...
gi 302142768 416 TRLFGADPpvlYVLHYLGL-KPW 437
Cdd:cd04194  227 LEEARKNP---VIIHYTGSdKPW 246
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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