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Conserved domains on  [gi|40041160|emb|CAE99332|]
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unnamed protein product, partial [Homo sapiens]

Protein Classification

uS12 family ribosomal protein( domain architecture ID 551)

uS12 family ribosomal protein such as plant 40S ribosomal protein S23, yeast mitochondrial 37S ribosomal protein S12, and chloroplast 30S ribosomal protein s12

Gene Ontology:  GO:0003735
PubMed:  24524803

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Ribosomal_S12_like super family cl00312
Ribosomal protein S12-like family; composed of prokaryotic 30S ribosomal protein S12, ...
 1-50 3.22e-24

Ribosomal protein S12-like family; composed of prokaryotic 30S ribosomal protein S12, eukaryotic 40S ribosomal protein S23 and similar proteins. S12 and S23 are located at the interface of the large and small ribosomal subunits, adjacent to the decoding center. They play an important role in translocation during the peptide elongation step of protein synthesis. They are also involved in important RNA and protein interactions. Ribosomal protein S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as a control element for the rRNA- and tRNA-driven movements of translocation. S23 interacts with domain III of the eukaryotic elongation factor 2 (eEF2), which catalyzes translocation. Mutations in S12 and S23 have been found to affect translational accuracy. Antibiotics such as streptomycin may also bind S12/S23 and cause the ribosome to misread the genetic code.


The actual alignment was detected with superfamily member PTZ00067:

Pssm-ID: 444826  Cd Length: 143  Bit Score: 87.11  E-value: 3.22e-24
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 40041160    1 MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGL 50
Cdd:PTZ00067   1 MGKPRGLRAARKLRRHRRVNRWADKEYKKAHLGTRYKANPFGGASHAKGI 50
 
Name Accession Description Interval E-value
PTZ00067 PTZ00067
40S ribosomal S23; Provisional
 1-50 3.22e-24

40S ribosomal S23; Provisional


Pssm-ID: 185422  Cd Length: 143  Bit Score: 87.11  E-value: 3.22e-24
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 40041160    1 MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGL 50
Cdd:PTZ00067   1 MGKPRGLRAARKLRRHRRVNRWADKEYKKAHLGTRYKANPFGGASHAKGI 50
Ribosomal_S23 cd03367
S12-like family, 40S ribosomal protein S23 subfamily; S23 is located at the interface of the ...
 26-52 4.51e-09

S12-like family, 40S ribosomal protein S23 subfamily; S23 is located at the interface of the large and small ribosomal subunits of eukaryotes, adjacent to the decoding center. It interacts with domain III of the eukaryotic elongation factor 2 (eEF2), which catalyzes the translocation of the growing peptidyl-tRNA to the P site to make room for the next aminoacyl-tRNA at the A (acceptor) site. Through its interaction with eEF2, S23 may play an important role in translocation. Also members of this subfamily are the archaeal 30S ribosomal S12 proteins. Prokaryotic S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as control element for the rRNA- and tRNA-driven movements of translocation. S12 and S23 are also implicated in translation accuracy. Antibiotics such as streptomycin bind S12/S23 and cause the ribosome to misread the genetic code.


Pssm-ID: 239465  Cd Length: 115  Bit Score: 47.65  E-value: 4.51e-09
                        10        20
                ....*....|....*....|....*..
gi 40041160  26 QYKKAHLGTALKANPFGGASHAKGLAI 52
Cdd:cd03367   1 RYKKRHLGTKYKADPLGGAPHAKGIVL 27
 
Name Accession Description Interval E-value
PTZ00067 PTZ00067
40S ribosomal S23; Provisional
 1-50 3.22e-24

40S ribosomal S23; Provisional


Pssm-ID: 185422  Cd Length: 143  Bit Score: 87.11  E-value: 3.22e-24
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 40041160    1 MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGL 50
Cdd:PTZ00067   1 MGKPRGLRAARKLRRHRRVNRWADKEYKKAHLGTRYKANPFGGASHAKGI 50
Ribosomal_S23 cd03367
S12-like family, 40S ribosomal protein S23 subfamily; S23 is located at the interface of the ...
 26-52 4.51e-09

S12-like family, 40S ribosomal protein S23 subfamily; S23 is located at the interface of the large and small ribosomal subunits of eukaryotes, adjacent to the decoding center. It interacts with domain III of the eukaryotic elongation factor 2 (eEF2), which catalyzes the translocation of the growing peptidyl-tRNA to the P site to make room for the next aminoacyl-tRNA at the A (acceptor) site. Through its interaction with eEF2, S23 may play an important role in translocation. Also members of this subfamily are the archaeal 30S ribosomal S12 proteins. Prokaryotic S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as control element for the rRNA- and tRNA-driven movements of translocation. S12 and S23 are also implicated in translation accuracy. Antibiotics such as streptomycin bind S12/S23 and cause the ribosome to misread the genetic code.


Pssm-ID: 239465  Cd Length: 115  Bit Score: 47.65  E-value: 4.51e-09
                        10        20
                ....*....|....*....|....*..
gi 40041160  26 QYKKAHLGTALKANPFGGASHAKGLAI 52
Cdd:cd03367   1 RYKKRHLGTKYKADPLGGAPHAKGIVL 27
rps12P PRK04211
30S ribosomal protein S12P; Reviewed
 5-52 1.29e-08

30S ribosomal protein S12P; Reviewed


Pssm-ID: 235257  Cd Length: 145  Bit Score: 47.25  E-value: 1.29e-08
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 40041160    5 RGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGLAI 52
Cdd:PRK04211   6 NGLFAARKLKLKRKKFRWSDRRYKRRMLGLKEKADPLEGAPMARGIVL 53
Ribosomal_S12_like cd00319
Ribosomal protein S12-like family; composed of prokaryotic 30S ribosomal protein S12, ...
 33-52 5.57e-04

Ribosomal protein S12-like family; composed of prokaryotic 30S ribosomal protein S12, eukaryotic 40S ribosomal protein S23 and similar proteins. S12 and S23 are located at the interface of the large and small ribosomal subunits, adjacent to the decoding center. They play an important role in translocation during the peptide elongation step of protein synthesis. They are also involved in important RNA and protein interactions. Ribosomal protein S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as a control element for the rRNA- and tRNA-driven movements of translocation. S23 interacts with domain III of the eukaryotic elongation factor 2 (eEF2), which catalyzes translocation. Mutations in S12 and S23 have been found to affect translational accuracy. Antibiotics such as streptomycin may also bind S12/S23 and cause the ribosome to misread the genetic code.


Pssm-ID: 238196  Cd Length: 95  Bit Score: 34.30  E-value: 5.57e-04
                       10        20
               ....*....|....*....|
gi 40041160 33 GTALKANPFGGASHAKGLAI 52
Cdd:cd00319  1 RKKSKVPALKGAPFRRGVCT 20
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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