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Conserved domains on  [gi|74226178|dbj|BAE25287|]
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unnamed protein product [Mus musculus]

Protein Classification

KRAB domain-containing zinc finger protein( domain architecture ID 12204268)

KRAB (Kruppel-associated box) domain-containing zinc finger protein (KRAB-ZFP) plays important roles in cell differentiation and organ development, and in regulating viral replication and transcription

CATH:  3.30.160.60
Gene Ontology:  GO:0003700|GO:0046872
PubMed:  22803940
SCOP:  4003583

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
KRAB smart00349
krueppel associated box;
5-63 2.28e-23

krueppel associated box;


:

Pssm-ID: 214630 [Multi-domain]  Cd Length: 61  Bit Score: 90.73  E-value: 2.28e-23
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 74226178      5 LSFWDVVIYFSAEEWEYLGPAQWKLYRDVTLENYNNFVFLDLVSSTPYLVRFLEQIQEP 63
Cdd:smart00349   1 VTFEDVAVYFTQEEWEQLDPAQKNLYRDVMLENYSNLVSLGFQVPKPDLISQLEQGEEP 59
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
26-268 2.58e-07

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 51.62  E-value: 2.58e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178  26 QWKLYRDVTLENYNNFVFLDLVSSTPYLVRFLEQIQEPSDVKSQADISmysggkcyTCKECGNIFVGKEVFQNHLIIHLG 105
Cdd:COG5048 208 SYSIPSSSSDQNLENSSSSLPLTTNSQLSPKSLLSQSPSSLSSSDSSS--------SASESPRSSLPTASSQSSSPNESD 279

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 106 -------GKPYKCEECGKSFRFPSLLSQHK--LIHTGE--KPYKC--ETCNRAFHVPSKLFQHKIIHTKAKLYKC--EVC 170
Cdd:COG5048 280 sssekgfSLPIKSKQCNISFSRSSPLTRHLrsVNHSGEslKPFSCpySLCGKLFSRNDALKRHILLHTSISPAKEklLNS 359

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 171 GKAFN-----------YSSRLLHHRTIHTGEKPYKCEVCGKAFHSSPSLYQHMGihTGEKPCKCDKCGKAFRFPSLLLKH 239
Cdd:COG5048 360 SSKFSpllnneppqslQQYKDLKNDKKSETLSNSCIRNFKRDSNLSLHIITHLS--FRPYNCKNPPCSKSFNRHYNLIPH 437

                       250       260
                ....*....|....*....|....*....
gi 74226178 240 KISHTNQKPYKCKICGDFLRSQSSLHEHK 268
Cdd:COG5048 438 KKIHTNHAPLLCSILKSFRRDLDLSNHGK 466
 
Name Accession Description Interval E-value
KRAB smart00349
krueppel associated box;
5-63 2.28e-23

krueppel associated box;


Pssm-ID: 214630 [Multi-domain]  Cd Length: 61  Bit Score: 90.73  E-value: 2.28e-23
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 74226178      5 LSFWDVVIYFSAEEWEYLGPAQWKLYRDVTLENYNNFVFLDLVSSTPYLVRFLEQIQEP 63
Cdd:smart00349   1 VTFEDVAVYFTQEEWEQLDPAQKNLYRDVMLENYSNLVSLGFQVPKPDLISQLEQGEEP 59
KRAB pfam01352
KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc ...
 4-45 2.81e-17

KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc finger proteins containing C2H2 fingers. The KRAB domain is found to be involved in protein-protein interactions. The KRAB domain is generally encoded by two exons. The regions coded by the two exons are known as KRAB-A and KRAB-B. The A box plays an important role in repression by binding to corepressors, while the B box is thought to enhance this repression brought about by the A box. KRAB-containing proteins are thought to have critical functions in cell proliferation and differentiation, apoptosis and neoplastic transformation.


Pssm-ID: 460171  Cd Length: 42  Bit Score: 73.66  E-value: 2.81e-17
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 74226178     4 MLSFWDVVIYFSAEEWEYLGPAQWKLYRDVTLENYNNFVFLD 45
Cdd:pfam01352   1 SVTFEDVAVDFTQEEWALLDPAQRNLYRDVMLENYRNLVSLG 42
KRAB_A-box cd07765
KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression ...
 5-44 4.08e-13

KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression module, found in a subgroup of the zinc finger proteins (ZFPs) of the C2H2 family, KRAB-ZFPs. KRAB-ZFPs comprise the largest group of transcriptional regulators in mammals, and are only found in tetrapods. These proteins have been shown to play important roles in cell differentiation and organ development, and in regulating viral replication and transcription. A KRAB domain may consist of an A-box, or of an A-box plus either a B-box, a divergent B-box (b), or a C-box. Only the A-box is included in this model. The A-box is needed for repression, the B- and C- boxes are not. KRAB-ZFPs have one or two KRAB domains at their amino-terminal end, and multiple C2H2 zinc finger motifs at their C-termini. Some KRAB-ZFPs also contain a SCAN domain which mediates homo- and hetero-oligomerization. The KRAB domain is a protein-protein interaction module which represses transcription through recruiting corepressors. A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta , KRAB-A interacting protein , and tripartite motif protein 28). The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation.


Pssm-ID: 143639  Cd Length: 40  Bit Score: 62.57  E-value: 4.08e-13
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 74226178   5 LSFWDVVIYFSAEEWEYLGPAQWKLYRDVTLENYNNFVFL 44
Cdd:cd07765   1 VTFEDVAVYFSQEEWELLDPAQRDLYRDVMLENYENLVSL 40
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
26-268 2.58e-07

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 51.62  E-value: 2.58e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178  26 QWKLYRDVTLENYNNFVFLDLVSSTPYLVRFLEQIQEPSDVKSQADISmysggkcyTCKECGNIFVGKEVFQNHLIIHLG 105
Cdd:COG5048 208 SYSIPSSSSDQNLENSSSSLPLTTNSQLSPKSLLSQSPSSLSSSDSSS--------SASESPRSSLPTASSQSSSPNESD 279

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 106 -------GKPYKCEECGKSFRFPSLLSQHK--LIHTGE--KPYKC--ETCNRAFHVPSKLFQHKIIHTKAKLYKC--EVC 170
Cdd:COG5048 280 sssekgfSLPIKSKQCNISFSRSSPLTRHLrsVNHSGEslKPFSCpySLCGKLFSRNDALKRHILLHTSISPAKEklLNS 359

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 171 GKAFN-----------YSSRLLHHRTIHTGEKPYKCEVCGKAFHSSPSLYQHMGihTGEKPCKCDKCGKAFRFPSLLLKH 239
Cdd:COG5048 360 SSKFSpllnneppqslQQYKDLKNDKKSETLSNSCIRNFKRDSNLSLHIITHLS--FRPYNCKNPPCSKSFNRHYNLIPH 437

                       250       260
                ....*....|....*....|....*....
gi 74226178 240 KISHTNQKPYKCKICGDFLRSQSSLHEHK 268
Cdd:COG5048 438 KKIHTNHAPLLCSILKSFRRDLDLSNHGK 466
PHA00733 PHA00733
hypothetical protein
 192-239 4.38e-03

hypothetical protein


Pssm-ID: 177301  Cd Length: 128  Bit Score: 36.78  E-value: 4.38e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 74226178  192 PYKCEVCGKAFHSSPSLYQHmgIHTGEKPCKCDKCGKAFRFPSLLLKH 239
Cdd:PHA00733  73 PYVCPLCLMPFSSSVSLKQH--IRYTEHSKVCPVCGKEFRNTDSTLDH 118
zf-H2C2_2 pfam13465
Zinc-finger double domain;
183-202 8.22e-03

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 33.50  E-value: 8.22e-03
                          10        20
                  ....*....|....*....|
gi 74226178   183 HRTIHTGEKPYKCEVCGKAF 202
Cdd:pfam13465   5 HMRTHTGEKPYKCPECGKSF 24
 
Name Accession Description Interval E-value
KRAB smart00349
krueppel associated box;
5-63 2.28e-23

krueppel associated box;


Pssm-ID: 214630 [Multi-domain]  Cd Length: 61  Bit Score: 90.73  E-value: 2.28e-23
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 74226178      5 LSFWDVVIYFSAEEWEYLGPAQWKLYRDVTLENYNNFVFLDLVSSTPYLVRFLEQIQEP 63
Cdd:smart00349   1 VTFEDVAVYFTQEEWEQLDPAQKNLYRDVMLENYSNLVSLGFQVPKPDLISQLEQGEEP 59
KRAB pfam01352
KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc ...
 4-45 2.81e-17

KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc finger proteins containing C2H2 fingers. The KRAB domain is found to be involved in protein-protein interactions. The KRAB domain is generally encoded by two exons. The regions coded by the two exons are known as KRAB-A and KRAB-B. The A box plays an important role in repression by binding to corepressors, while the B box is thought to enhance this repression brought about by the A box. KRAB-containing proteins are thought to have critical functions in cell proliferation and differentiation, apoptosis and neoplastic transformation.


Pssm-ID: 460171  Cd Length: 42  Bit Score: 73.66  E-value: 2.81e-17
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 74226178     4 MLSFWDVVIYFSAEEWEYLGPAQWKLYRDVTLENYNNFVFLD 45
Cdd:pfam01352   1 SVTFEDVAVDFTQEEWALLDPAQRNLYRDVMLENYRNLVSLG 42
KRAB_A-box cd07765
KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression ...
 5-44 4.08e-13

KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression module, found in a subgroup of the zinc finger proteins (ZFPs) of the C2H2 family, KRAB-ZFPs. KRAB-ZFPs comprise the largest group of transcriptional regulators in mammals, and are only found in tetrapods. These proteins have been shown to play important roles in cell differentiation and organ development, and in regulating viral replication and transcription. A KRAB domain may consist of an A-box, or of an A-box plus either a B-box, a divergent B-box (b), or a C-box. Only the A-box is included in this model. The A-box is needed for repression, the B- and C- boxes are not. KRAB-ZFPs have one or two KRAB domains at their amino-terminal end, and multiple C2H2 zinc finger motifs at their C-termini. Some KRAB-ZFPs also contain a SCAN domain which mediates homo- and hetero-oligomerization. The KRAB domain is a protein-protein interaction module which represses transcription through recruiting corepressors. A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta , KRAB-A interacting protein , and tripartite motif protein 28). The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation.


Pssm-ID: 143639  Cd Length: 40  Bit Score: 62.57  E-value: 4.08e-13
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 74226178   5 LSFWDVVIYFSAEEWEYLGPAQWKLYRDVTLENYNNFVFL 44
Cdd:cd07765   1 VTFEDVAVYFSQEEWELLDPAQRDLYRDVMLENYENLVSL 40
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
26-268 2.58e-07

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 51.62  E-value: 2.58e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178  26 QWKLYRDVTLENYNNFVFLDLVSSTPYLVRFLEQIQEPSDVKSQADISmysggkcyTCKECGNIFVGKEVFQNHLIIHLG 105
Cdd:COG5048 208 SYSIPSSSSDQNLENSSSSLPLTTNSQLSPKSLLSQSPSSLSSSDSSS--------SASESPRSSLPTASSQSSSPNESD 279

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 106 -------GKPYKCEECGKSFRFPSLLSQHK--LIHTGE--KPYKC--ETCNRAFHVPSKLFQHKIIHTKAKLYKC--EVC 170
Cdd:COG5048 280 sssekgfSLPIKSKQCNISFSRSSPLTRHLrsVNHSGEslKPFSCpySLCGKLFSRNDALKRHILLHTSISPAKEklLNS 359

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 171 GKAFN-----------YSSRLLHHRTIHTGEKPYKCEVCGKAFHSSPSLYQHMGihTGEKPCKCDKCGKAFRFPSLLLKH 239
Cdd:COG5048 360 SSKFSpllnneppqslQQYKDLKNDKKSETLSNSCIRNFKRDSNLSLHIITHLS--FRPYNCKNPPCSKSFNRHYNLIPH 437

                       250       260
                ....*....|....*....|....*....
gi 74226178 240 KISHTNQKPYKCKICGDFLRSQSSLHEHK 268
Cdd:COG5048 438 KKIHTNHAPLLCSILKSFRRDLDLSNHGK 466
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 104-185 2.75e-05

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 45.48  E-value: 2.75e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 104 LGGKPYKCE--ECGKSFRFPSLLSQHKLihTGEkpykcetCNRAFHVPSKLFQHKIIHTKAKLYKCEVCGKAFNYSSRLL 181
Cdd:COG5189 345 KDGKPYKCPveGCNKKYKNQNGLKYHML--HGH-------QNQKLHENPSPEKMNIFSAKDKPYRCEVCDKRYKNLNGLK 415


                ....
gi 74226178 182 HHRT 185
Cdd:COG5189 416 YHRK 419
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
163-304 4.01e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 44.69  E-value: 4.01e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 163 KLYKCEVCGKAFNYSSRLLHHR--TIHTGE--KPYKC--EVCGKAFHSSPSLYQHMGIHTG--EKPCKCDKCGKAFRFPS 234
Cdd:COG5048 288 LPIKSKQCNISFSRSSPLTRHLrsVNHSGEslKPFSCpySLCGKLFSRNDALKRHILLHTSisPAKEKLLNSSSKFSPLL 367

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 74226178 235 ------LLLKHKISHTNQK---PYKCKICGDFLRSQSSLHEHKLMHKGEKLYKCDVCGKAFEYPSRLSKHKKIHTREKP 304
Cdd:COG5048 368 nneppqSLQQYKDLKNDKKsetLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAP 446
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 175-268 1.92e-04

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 42.78  E-value: 1.92e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 175 NYSSRLLHHRtihtGEKPYKCEV--CGKAFHSSPSLYQHMgIHTGEKPckcdkcgKAFRFPSLLlKHKISHTNQKPYKCK 252
Cdd:COG5189 336 DTPSRMLKVK----DGKPYKCPVegCNKKYKNQNGLKYHM-LHGHQNQ-------KLHENPSPE-KMNIFSAKDKPYRCE 402

                        90
                ....*....|....*.
gi 74226178 253 ICGDFLRSQSSLHEHK 268
Cdd:COG5189 403 VCDKRYKNLNGLKYHR 418
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 133-213 1.17e-03

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 40.09  E-value: 1.17e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 133 GEKPYKCET--CNRAFHVPSKLFQHKII-HTKAKLYKCEVCGKafnyssrllhHRTIHTGEKPYKCEVCGKAFHSSPSLY 209
Cdd:COG5189 346 DGKPYKCPVegCNKKYKNQNGLKYHMLHgHQNQKLHENPSPEK----------MNIFSAKDKPYRCEVCDKRYKNLNGLK 415


                ....
gi 74226178 210 QHMG 213
Cdd:COG5189 416 YHRK 419
PHA00733 PHA00733
hypothetical protein
 192-239 4.38e-03

hypothetical protein


Pssm-ID: 177301  Cd Length: 128  Bit Score: 36.78  E-value: 4.38e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 74226178  192 PYKCEVCGKAFHSSPSLYQHmgIHTGEKPCKCDKCGKAFRFPSLLLKH 239
Cdd:PHA00733  73 PYVCPLCLMPFSSSVSLKQH--IRYTEHSKVCPVCGKEFRNTDSTLDH 118
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
135-248 4.75e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 38.52  E-value: 4.75e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74226178 135 KPYKCETCNRAFHVPSKLFQHKIIHTKAKLYKCEVCGKAFNYSSR--LLHHRTIHTGEKPYKCEVCGKAFHSSPSLYQHm 212
Cdd:COG5048  32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPleLSRHLRTHHNNPSDLNSKSLPLSNSKASSSSL- 110

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 74226178 213 gihtgekpckcDKCGKAFRFPSLLLKHKISHTNQKP 248
Cdd:COG5048 111 -----------SSSSSNSNDNNLLSSHSLPPSSRDP 135
zf-H2C2_2 pfam13465
Zinc-finger double domain;
183-202 8.22e-03

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 33.50  E-value: 8.22e-03
                          10        20
                  ....*....|....*....|
gi 74226178   183 HRTIHTGEKPYKCEVCGKAF 202
Cdd:pfam13465   5 HMRTHTGEKPYKCPECGKSF 24
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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