|
Name |
Accession |
Description |
Interval |
E-value |
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
79-743 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 1443.55 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 79 VLGESLASEENDLVFPSKEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKW-GDQVFSENLDVRKGPISIE 157
Cdd:PLN02654 1 VLGESLASEENDLVFPSKDFSAQALVSSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWeGDEVCSENLDVRKGPISIE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 158 WFKGGITNICYNCLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPI 237
Cdd:PLN02654 81 WFKGGKTNICYNCLDRNVEAGNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPI 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 238 AMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLA 317
Cdd:PLN02654 161 AMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVSVGICLTYENQLA 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 318 TTRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVY 397
Cdd:PLN02654 241 MKREDTKWQEGRDVWWQDVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVY 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 398 WCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLR 477
Cdd:PLN02654 321 WCTADCGWITGHSYVTYGPMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLR 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 478 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECS 557
Cdd:PLN02654 401 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECS 480
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 558 GYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 637
Cdd:PLN02654 481 GYLCVKKSWPGAFRTLYGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 560
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 638 CAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASR 717
Cdd:PLN02654 561 CAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASR 640
|
650 660
....*....|....*....|....*.
gi 378548265 718 QLEELGDTSTLADPSVVDQLIALADV 743
Cdd:PLN02654 641 QLDELGDTSTLADPGVVDQLIALADS 666
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
108-730 |
0e+00 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 1171.57 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 108 QQYMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVfsenLDVRKGPISIEWFKGGITNICYNCLDKNVEAgLGDKTAIHW 187
Cdd:cd05966 1 EQYKELYKQSIEDPEEFWGEIAKELDWFKPW-DKV----LDWSKGPPFIKWFEGGKLNISYNCLDRHLKE-RGDKVAIIW 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 188 EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 267
Cdd:cd05966 75 EGDEPDQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRIND 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 268 CKPNVILTCNAVKRGPKTINLKAIVDAALDQsskdGVSVGICLTYDNslatTRENTKWQNGRDVWWQDVISQYPTSCEVE 347
Cdd:cd05966 155 AQCKLVITADGGYRGGKVIPLKEIVDEALEK----CPSVEKVLVVKR----TGGEVPMTEGRDLWWHDLMAKQSPECEPE 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 WVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFE 427
Cdd:cd05966 227 WMDSEDPLFILYTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVMFE 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 GAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISD 507
Cdd:cd05966 307 GTPTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPEAWMWYYEVIGKERCPIVD 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 508 TWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKP 587
Cdd:cd05966 387 TWWQTETGGIMITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGMARTIYGDHERYEDTYFSK 466
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPY 667
Cdd:cd05966 467 FPGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEP 546
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 668 SEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQlEELGDTSTLAD 730
Cdd:cd05966 547 SDELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAGE-EELGDTSTLAD 608
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
94-739 |
0e+00 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 1137.56 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 94 PSKEFSGQALVSsPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVFSENldvrkgPISIEWFKGGITNICYNCLDK 173
Cdd:PRK00174 4 PPAEFAANALID-MEQYKALYQESVEDPEGFWAEQAKRLDWFKPF-DTVLDWN------APFIKWFEDGELNVSYNCLDR 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 174 NVEAGlGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVF 253
Cdd:PRK00174 76 HLKTR-GDKVAIIWEGDDPGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVVF 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 254 AGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDqsskdgvsvgICLTYDNSLATTR--ENTKWQNGRDV 331
Cdd:PRK00174 155 GGFSAEALADRIIDAGAKLVITADEGVRGGKPIPLKANVDEALA----------NCPSVEKVIVVRRtgGDVDWVEGRDL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 332 WWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSY 411
Cdd:PRK00174 225 WWHELVAGASDECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWVTGHSY 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 412 VTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAW 491
Cdd:PRK00174 305 IVYGPLANGATTLMFEGVPNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKKYDLSSLRLLGSVGEPINPEAW 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 492 RWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFR 571
Cdd:PRK00174 385 EWYYKVVGGERCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDPWPGMMR 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 572 TLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVK 651
Cdd:PRK00174 465 TIYGDHERFVKTYFSTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIK 544
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 652 GQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQlEELGDTSTLADP 731
Cdd:PRK00174 545 GQGIYAFVTLKGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRILRKIAEGE-EILGDTSTLADP 623
|
....*...
gi 378548265 732 SVVDQLIA 739
Cdd:PRK00174 624 SVVEKLIE 631
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
104-739 |
0e+00 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 1061.09 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 104 VSSPQQYMEMHKRSMDDPAAFWSDIASE-FYWKQKWgDQVFSENLdvrkgPISIEWFKGGITNICYNCLDKNVEAgLGDK 182
Cdd:TIGR02188 1 IANLEQYKELYEESIEDPDKFWAKLARElLDWFKPF-TKVLDWSF-----PPFYKWFVGGELNVSYNCVDRHLEA-RPDK 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 183 TAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLA 262
Cdd:TIGR02188 74 VAIIWEGDEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEALA 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 263 QRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSskdGVSVGICLTYDNslaTTRENTKWQNGRDVWWQDVISQYPT 342
Cdd:TIGR02188 154 DRINDAGAKLVITADEGLRGGKVIPLKAIVDEALEKC---PVSVEHVLVVRR---TGNPVVPWVEGRDVWWHDLMAKASA 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 343 SCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGAT 422
Cdd:TIGR02188 228 YCEPEPMDSEDPLFILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCTADVGWITGHSYIVYGPLANGAT 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 423 VVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSR 502
Cdd:TIGR02188 308 TVMFEGVPTYPDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLGSVGEPINPEAWMWYYKVVGKER 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 503 CPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEG-ECSGYLCVKGSWPGAFRTLFGDHERYE 581
Cdd:TIGR02188 388 CPIVDTWWQTETGGIMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGpGEGGYLVIKQPWPGMLRTIYGDHERFV 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 582 TTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTL 661
Cdd:TIGR02188 468 DTYFSPFPGYYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTL 547
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 662 LEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEELGDTSTLADPSVVDQLIA 739
Cdd:TIGR02188 548 KDGYEPDDELRKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEAEILGDTSTLEDPSVVEELIE 625
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
157-739 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 890.62 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 157 EWFKGGITNICYNCLDKNVEaGLGDKTAIHWEGnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELP 236
Cdd:COG0365 1 RWFVGGRLNIAYNCLDRHAE-GRGDKVALIWEG-EDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAV 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 237 IAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSkdgvSVGICLTYDnsl 316
Cdd:COG0365 79 IAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEALEELP----SLEHVIVVG--- 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 317 attRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDV 396
Cdd:COG0365 152 ---RTGADVPMEGDLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDV 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 397 YWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSL 476
Cdd:COG0365 229 FWCTADIGWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSL 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 477 RVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPGaWPQKPGSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:COG0365 309 RLLGSAGEPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPVPGYDVAVVDEDGNPVPPGE 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:COG0365 385 EGELVIKGPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHP 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIAS 716
Cdd:COG0365 465 AVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAE 544
|
570 580
....*....|....*....|...
gi 378548265 717 RqlEELGDTSTLADPSVVDQLIA 739
Cdd:COG0365 545 G--RPLGDTSTLEDPEALDEIKE 565
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
110-707 |
0e+00 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 722.44 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 110 YMEMHKRSMDDPAAFWSDIASEFYWKqkWGDQVfSENLDVRKGPISIEWFKGGITNICYNCLDKNVEAGlGDKTAIHWEG 189
Cdd:cd17634 1 YETKYRQSINDPDTFWGEAGKILDWI--TPYQK-VKNTSFAPGAPSIKWFEDATLNLAANALDRHLREN-GDRTAIIYEG 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 190 NELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:cd17634 77 DDTSQSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSS 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAVKRGPKTINLKAIVDAALDQSskdGVSVGICLTYDNslatTRENTKWQNGRDVWWQDVISQYPTSCEVEWV 349
Cdd:cd17634 157 SRLLITADGGVRAGRSVPLKKNVDDALNPN---VTSVEHVIVLKR----TGSDIDWQEGRDLWWRDLIAKASPEHQPEAM 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGA 429
Cdd:cd17634 230 NAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACGATTLLYEGV 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 PNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTW 509
Cdd:cd17634 310 PNWPTPARMWQVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSLRILGSVGEPINPEAYEWYWKKIGKEKCPVVDTW 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 510 WQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFA 589
Cdd:cd17634 390 WQTETGGFMITPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQTRTLFGDHERFEQTYFSTFK 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 590 GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSE 669
Cdd:cd17634 470 GMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSP 549
|
570 580 590
....*....|....*....|....*....|....*...
gi 378548265 670 ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIM 707
Cdd:cd17634 550 ELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
110-737 |
0e+00 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 645.53 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 110 YMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVFSenldvRKGPISIEWFKGGITNICYNCLDKNVEAGLGDKTAIHWEG 189
Cdd:cd05967 1 YEEVYARSIAEPEAFWAEQARLIDWFKPP-EKILD-----NSNPPFTRWFVGGRLNTCYNALDRHVEAGRGDQIALIYDS 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 190 NELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:cd05967 75 PVTGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDAK 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVgicLTYDNSLATTRENTKwqnGRDVWWQDVISQYpTSCEVEWV 349
Cdd:cd05967 155 PKLIVTASCGIEPGKVVPYKPLLDKALELSGHKPHHV---LVLNRPQVPADLTKP---GRDLDWSELLAKA-EPVDCVPV 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGA 429
Cdd:cd05967 228 AATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWAASDVGWVVGHSYIVYGPLLHGATTVLYEGK 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 P-NYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDD--KFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPIS 506
Cdd:cd05967 308 PvGTPDPGAFWRVIEKYQVNALFTAPTAIRAIRKEDPdgKYIKKYDLSSLRTLFLAGERLDPPTLEWAENTLG---VPVI 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 507 DTWWQTETGGFMITPLPG--AWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSW-PGAFRTLFGDHERYETT 583
Cdd:cd05967 385 DHWWQTETGWPITANPVGlePLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLPLpPGCLLTLWKNDERFKKL 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 584 YFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLE 663
Cdd:cd05967 465 YLSKFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKE 544
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 664 GVPYS-EELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIAsrQLEELGDTSTLADPSVVDQL 737
Cdd:cd05967 545 GVKITaEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIA--DGEDYTIPSTIEDPSVLDEI 617
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
108-737 |
0e+00 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 581.52 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 108 QQYMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVfsenLDVRKGPISiEWFKGGITNICYNCLDKNVEAGlGDKTAIHW 187
Cdd:PRK10524 2 MSYSEFYQRSIDDPEAFWAEQARRIDWQTPF-TQV----LDYSNPPFA-RWFVGGRTNLCHNAVDRHLAKR-PEQLALIA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 188 EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 267
Cdd:PRK10524 75 VSTETDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDD 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 268 CKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVgicLTYDNSLATTRentkWQNGRDVWWQDVISQY-PTSCEV 346
Cdd:PRK10524 155 AKPVLIVSADAGSRGGKVVPYKPLLDEAIALAQHKPRHV---LLVDRGLAPMA----RVAGRDVDYATLRAQHlGARVPV 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 347 EWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVF 426
Cdd:PRK10524 228 EWLESNEPSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETFFCASDIGWVVGHSYIVYAPLLAGMATIMY 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 EGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPIS 506
Cdd:PRK10524 308 EGLPTRPDAGIWWRIVEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSSLRALFLAGEPLDEPTASWISEALG---VPVI 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 507 DTWWQTETGGFMITPLPG--AWPQKPGSATFPFFGVQPVIVDEK-GNEIEGECSGYLCVKGSW-PGAFRTLFGDHERYET 582
Cdd:PRK10524 385 DNYWQTETGWPILAIARGveDRPTRLGSPGVPMYGYNVKLLNEVtGEPCGPNEKGVLVIEGPLpPGCMQTVWGDDDRFVK 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 583 TYFKPFAG-YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTL 661
Cdd:PRK10524 465 TYWSLFGRqVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVP 544
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 662 LEGVPY-SEELRKSL--VLM--VRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQleELGDTSTLADPSVVDQ 736
Cdd:PRK10524 545 KDSDSLaDREARLALekEIMalVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQAIAEGR--DPGDLTTIEDPAALQQ 622
|
.
gi 378548265 737 L 737
Cdd:PRK10524 623 I 623
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
108-731 |
2.06e-174 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 513.19 E-value: 2.06e-174
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 108 QQYMEMHKRSMDDPAAFWSDIASEF-YWKQKWGDQVfsenLDVRKGPISIEWFKGGITNICYNCLDKNVeAGLGDKTAIH 186
Cdd:cd05968 7 PDLEAFLERSAEDNAWFWGEFVKDVgIEWYEPPYQT----LDLSGGKPWAAWFVGGRMNIVEQLLDKWL-ADTRTRPALR 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 187 WEGnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIV 266
Cdd:cd05968 82 WEG-EDGTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQ 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 267 DCKPNVILTCNAVKRGPKTINLKAIVDAALDQsskdgvsvgiCLTYDNSLATTR--ENTKWQNGRDVWWQDVISQYPTsc 344
Cdd:cd05968 161 DAEAKALITADGFTRRGREVNLKEEADKACAQ----------CPTVEKVVVVRHlgNDFTPAKGRDLSYDEEKETAGD-- 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 345 EVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTD-VYWCTaDCGWITGhSYVTYGPMLNGATV 423
Cdd:cd05968 229 GAERTESEDPLMIIYTSGTTGKPKGTVHVHAGFPLKAAQDMYFQFDLKPGDlLTWFT-DLGWMMG-PWLIFGGLILGATM 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRC 503
Cdd:cd05968 307 VLYDGAPDHPKADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLRVLGSTGEPWNPEPWNWLFETVGKGRN 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTET-----GGFMITPLpgawpqKPGSATFPFFGVQPVIVDEKGNEIEGEcSGYLCVKGSWPGAFRTLFGDHE 578
Cdd:cd05968 387 PIINYSGGTEIsggilGNVLIKPI------KPSSFNGPVPGMKADVLDESGKPARPE-VGELVLLAPWPGMTRGFWRDED 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 579 RYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAF 658
Cdd:cd05968 460 RYLETYWSRFDNVWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCF 539
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 659 VTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRkiASRQLEELGDTSTLADP 731
Cdd:cd05968 540 VVLKPGVTPTEALAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIR--AAYLGKELGDLSSLENP 610
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
148-730 |
9.51e-174 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 510.21 E-value: 9.51e-174
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 148 DVRKgpiSIEWFKGGITNICYNCLDKNVEAGLGDKTAIHWEGNELGVDasLTYSELLQRVCQLANYLKDNGVKKGDAVVI 227
Cdd:PRK04319 29 EVEK---EFSWLETGKVNIAYEAIDRHADGGRKDKVALRYLDASRKEK--YTYKELKELSNKFANVLKELGVEKGDRVFI 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 228 YLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAV---KRGPKTINLKAIVDAALDQSSKDGv 304
Cdd:PRK04319 104 FMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALlerKPADDLPSLKHVLLVGEDVEEGPG- 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 305 svgiCLTYDNSLAttrentkwqngrdvwwqdvisQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATT 384
Cdd:PRK04319 183 ----TLDFNALME---------------------QASDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHNA-MLQHYQT 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 385 FKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDD 464
Cdd:PRK04319 237 GKYVLDLHEDDVYWCTADPGWVTGTSYGIFAPWLNGATNVIDGGRF---SPERWYRILEDYKVTVWYTAPTAIRMLMGAG 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 465 DKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPgAWPQKPGSATFPFFGVQPVI 544
Cdd:PRK04319 314 DDLVKKYDLSSLRHILSVGEPLNPEVVRWGMKVFG---LPIHDNWWMTETGGIMIANYP-AMDIKPGSMGKPLPGIEAAI 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 545 VDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETtYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIG 624
Cdd:PRK04319 390 VDDQGNELPPNRMGNLAIKKGWPSMMRGIWNNPEKYES-YFAG--DWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVG 466
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 625 TAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSG 704
Cdd:PRK04319 467 PFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSG 546
|
570 580
....*....|....*....|....*..
gi 378548265 705 KIMRRILRkiaSRQLE-ELGDTSTLAD 730
Cdd:PRK04319 547 KIMRRVLK---AWELGlPEGDLSTMED 570
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
198-716 |
2.09e-142 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 424.99 E-value: 2.09e-142
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCN 277
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AVKRgpKTinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDPLFL 357
Cdd:cd05969 81 ELYE--RT----------------------------------------------------------------DPEDPTLL 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPnypDPGR 437
Cdd:cd05969 95 HYTSGTTGTPKGVLHVHDA-MIFYYFTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEGRF---DAES 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 438 CWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGF 517
Cdd:cd05969 171 WYGIIERVKVTVWYTAPTAIRMLMKEGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFG---VPIHDTWWQTETGSI 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 518 MITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETtYFKpfAGYYFSGDG 597
Cdd:cd05969 248 MIANYPCM-PIKPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGWPSMFRGIWNDEERYKN-SFI--DGWYLTGDL 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 598 CSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVL 677
Cdd:cd05969 324 AYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIIN 403
|
490 500 510
....*....|....*....|....*....|....*....
gi 378548265 678 MVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIAS 716
Cdd:cd05969 404 FVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLKAKEL 442
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
198-713 |
2.75e-117 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 359.73 E-value: 2.75e-117
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIvdckpnviltcn 277
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRL------------ 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpKTINLKAIVdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewVDAEDPLFL 357
Cdd:cd05972 69 ------EAAGAKAIV--------------------------------------------------------TDAEDPALI 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGgYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNypDPGR 437
Cdd:cd05972 87 YFTSGTTGLPKGVLHTHS-YPLGHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRF--DAER 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 438 CWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGgF 517
Cdd:cd05972 164 ILELLERYGVTSFCGPPTAYRMLIKQD---LSSYKFSHLRLVVSAGEPLNPEVIEWWRAATG---LPIRDGYGQTETG-L 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 518 MITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFkpfAGYYFSGDG 597
Cdd:cd05972 237 TVGNFPDM-PVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPPGLFLGYVGDPEKTEASIR---GDYYLTGDR 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 598 CSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVL 677
Cdd:cd05972 313 AYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQG 392
|
490 500 510
....*....|....*....|....*....|....*.
gi 378548265 678 MVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05972 393 HVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
177-619 |
9.30e-113 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 347.76 E-value: 9.30e-113
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 177 AGLGDKTAIhwegnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGF 256
Cdd:pfam00501 6 ARTPDKTAL-----EVGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SADSLAQRIVDCKPNVILTCNAVKrgpktinlkaIVDAALDQSSKDGVSVGICLTYDNSLATtrentkwqngrDVWWQDV 336
Cdd:pfam00501 81 PAEELAYILEDSGAKVLITDDALK----------LEELLEALGKLEVVKLVLVLDRDPVLKE-----------EPLPEEA 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTgGYMIYTATTFK----YAFDYKSTDVYWCTADCGWITGHSYV 412
Cdd:pfam00501 140 KPADVPPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIKrvrpRGFGLGPDDRVLSTLPLFHDFGLSLG 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 413 TYGPMLNGATVVVFEGAPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPINPSAWR 492
Cdd:pfam00501 219 LLGPLLAGATVVLPPGFPA-LDPAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLS--SLRLVLSGGAPLPPELAR 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 493 WFFNVVGdsrCPISDTWWQTETGGFMITPLPGAWPQ-KPGSATFPFFGVQPVIVDEK-GNEIEGECSGYLCVKGswPGAF 570
Cdd:pfam00501 296 RFRELFG---GALVNGYGLTETTGVVTTPLPLDEDLrSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRG--PGVM 370
|
410 420 430 440
....*....|....*....|....*....|....*....|....*....
gi 378548265 571 RTLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVS 619
Cdd:pfam00501 371 KGYLNDPELTAEAFDED--GWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
180-721 |
5.00e-97 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 307.89 E-value: 5.00e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:COG0318 13 PDRPALVFGG------RRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPRLTAE 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTCnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisq 339
Cdd:COG0318 87 ELAYILEDSGARALVTA--------------------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 yptscevewvdaedplFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLN 419
Cdd:COG0318 104 ----------------LILYTSGTTGRPKGVMLTHRN-LLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLA 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVFEGapnyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRWFFNVVG 499
Cdd:COG0318 167 GATLVLLPR----FDPERVLELIERERVTVLFGVPTMLARLLRHPEF--ARYDLSSLRLVVSGGAPLPPELLERFEERFG 240
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dsrCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEI----EGEcsgyLCVKGswPGAFRTLFG 575
Cdd:COG0318 241 ---VRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPGVEVRIVDEDGRELppgeVGE----IVVRG--PNVMKGYWN 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHEryETTyfKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQG 654
Cdd:COG0318 312 DPE--ATA--EAFRdGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGER 387
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 655 IYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEE 721
Cdd:COG0318 388 VVAFVVLRPGAELDaEELRAFL----RERLARYKVPRRVEFVDELPRTASGKIDRRALRERYAAGALE 451
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
353-707 |
9.85e-89 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 282.25 E-value: 9.85e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYaFDYKSTDVYWCTADCGWItGHSYVTYGPMLNGATVVVFEGapny 432
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAAS-GGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPK---- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 433 PDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQT 512
Cdd:cd04433 75 FDPEAALELIEREKVTILLGVPTLLARLLKAPES--AGYDLSSLRALVSGGAPLPPELLERFEEAPG---IKLVNGYGLT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 513 ETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLfgdheRYETTYFKPFAGYY 592
Cdd:cd04433 150 ETGGTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWN-----NPEATAAVDEDGWY 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEEL 671
Cdd:cd04433 225 RTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGAdLDAEEL 304
|
330 340 350
....*....|....*....|....*....|....*.
gi 378548265 672 RKSlvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIM 707
Cdd:cd04433 305 RAH----VRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
110-735 |
6.04e-87 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 286.86 E-value: 6.04e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 110 YMEMHKRSMDDPAAFWSDIasefyWKqkWGDQVFS---ENLDVRKGPI-SIEWFKGGITNICYNCLDKnveAGLGDKTAI 185
Cdd:cd05943 19 YAALHRWSVDDPGAFWAAV-----WD--FSGVRGSkpyDVVVVSGRIMpGARWFPGARLNYAENLLRH---ADADDPAAI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 186 HweGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRI 265
Cdd:cd05943 89 Y--AAEDGERTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRF 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 266 VDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWqngrdVWWQDVISQYPT-SC 344
Cdd:cd05943 167 GQIEPKVLFAVDAYTYNGKRHDVREKVAELVKGLPSLLAVVVVPYTVAAGQPDLSKIAKA-----LTLEDFLATGAAgEL 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 345 EVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYgpMLNGATVV 424
Cdd:cd05943 242 EFEPLPFDHPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHILHCDLRPGDRLFYYTTCGWMMWNWLVSG--LAVGATIV 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 425 VFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrcp 504
Cdd:cd05943 320 LYDGSPFYPDTNALWDLADEEGITVFGTSAKYLDALEKAGLKPAETHDLSSLRTILSTGSPLKPESFDYVYDHIK----- 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 iSDTWWQTETGG------FMIT-PLpgaWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKG--SWPGAFrtlFG 575
Cdd:cd05943 395 -PDVLLASISGGtdiiscFVGGnPL---LPVYRGEIQCRGLGMAVEAFDEEGKPVWGEKGELVCTKPfpSMPVGF---WN 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHE--RYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:cd05943 468 DPDgsRYRAAYFAKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDE 547
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 654 GIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRqlEELGDTSTLADPSV 733
Cdd:cd05943 548 RVILFVKLREGVELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGKKVEVAVKKIIAG--RPVKNAGALANPES 625
|
..
gi 378548265 734 VD 735
Cdd:cd05943 626 LD 627
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
105-713 |
4.24e-80 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 268.92 E-value: 4.24e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 105 SSPQQYMEMHKRSMDDPAAFWSDIASEF-YWkQKWGDQVFSENldvrkgPISIEWFKGGITNICYNCLDKNVEAGLG-DK 182
Cdd:PTZ00237 5 SDPFDYENDSNYANSNPESFWDEVAKKYvHW-DKMYDKVYSGD------EIYPDWFKGGELNTCYNVLDIHVKNPLKrDQ 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 183 TAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLA 262
Cdd:PTZ00237 78 DALIYECPYLKKTIKLTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSLI 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 263 QRIVDCKPNVILTCNAVKRGPKTI----NLKA-------------------IVDAALDQSSKDGVSVGICLTYDNSLATT 319
Cdd:PTZ00237 158 DRIETITPKLIITTNYGILNDEIItftpNLKEaielstfkpsnvitlfrndITSESDLKKIETIPTIPNTLSWYDEIKKI 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 320 RENTkwqngrdvwwQDVISQYPTscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWC 399
Cdd:PTZ00237 238 KENN----------QSPFYEYVP------VESSHPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFS 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 400 TADCGWITGHSYVtYGPMLNGATVVVFEGA----PNYPDpgRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSR-- 473
Cdd:PTZ00237 302 HSSIGWVSFHGFL-YGSLSLGNTFVMFEGGiiknKHIED--DLWNTIEKHKVTHTLTLPKTIRYLIKTDPEATIIRSKyd 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 474 -KSLRVLGSVGEPINPSAWRWFFNVVgdsRCPISDTWWQTETGgfmITPLPG-AWPQKPGSAT-FPFFGVQPVIVDEKGN 550
Cdd:PTZ00237 379 lSNLKEIWCGGEVIEESIPEYIENKL---KIKSSRGYGQTEIG---ITYLYCyGHINIPYNATgVPSIFIKPSILSEDGK 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 551 EIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVES 630
Cdd:PTZ00237 453 ELNVNEIGEVAFKLPMPPSFATTFYKNDEKFKQLFSKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIET 532
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 631 ALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSE----ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKI 706
Cdd:PTZ00237 533 SILKHPLVLECCSIGIYDPDCYNVPIGLLVLKQDQSNQSidlnKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKI 612
|
....*..
gi 378548265 707 MRRILRK 713
Cdd:PTZ00237 613 PRQIISK 619
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
110-741 |
3.75e-79 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 266.66 E-value: 3.75e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 110 YMEMHKRSMDDPAAFWSDIASEFYWKQKWGDQVFsenLDVRKGPISiEWFKGGITNICYNCLdknvEAGLGDKTAIHWEG 189
Cdd:PRK03584 36 YAALWRWSVEDLEAFWQSVWDFFGVIGSTPYTVV---LAGRRMPGA-RWFPGARLNYAENLL----RHRRDDRPAIIFRG 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 190 nELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:PRK03584 108 -EDGPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQGVLDRFGQIE 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAVKRGPKTINLKAIVDAALDQ--SSKDGVSVgiclTYDNSLATTRENTKWqngrdVWWQDVISQYPTS-CEV 346
Cdd:PRK03584 187 PKVLIAVDGYRYGGKAFDRRAKVAELRAAlpSLEHVVVV----PYLGPAAAAAALPGA-----LLWEDFLAPAEAAeLEF 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 347 EWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVY-WCTAdCGWITGHSYVtyGPMLNGATVVV 425
Cdd:PRK03584 258 EPVPFDHPLWILYSSGTTGLPKCIVHGHGGILLEHLKELGLHCDLGPGDRFfWYTT-CGWMMWNWLV--SGLLVGATLVL 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 426 FEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrcpi 505
Cdd:PRK03584 335 YDGSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDACEKAGLVPGETHDLSALRTIGSTGSPLPPEGFDWVYEHVK------ 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 506 SDTWWQTETGGfmiTPLPGAW-------PQKPGSATFPFFGVQPVIVDEKGNEIEGEcSGYLCVKGSWPgAFRTLF---G 575
Cdd:PRK03584 409 ADVWLASISGG---TDICSCFvggnpllPVYRGEIQCRGLGMAVEAWDEDGRPVVGE-VGELVCTKPFP-SMPLGFwndP 483
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK03584 484 DGSRYRDAYFDTFPGVWRHGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPEVLDSLVIGQEWPDGDVRM 563
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 656 YAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIM----RRILRKiasRQLEELGDTSTLADP 731
Cdd:PRK03584 564 PLFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKKVelpvKKLLHG---RPVKKAVNRDALANP 640
|
650
....*....|
gi 378548265 732 SVVDQLIALA 741
Cdd:PRK03584 641 EALDWFADLA 650
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
198-713 |
8.36e-79 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 259.37 E-value: 8.36e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILtCN 277
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVV-TD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AVKRgpktinlkaivdAALDqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaEDPLFL 357
Cdd:cd05973 80 AANR------------HKLD------------------------------------------------------SDPFVM 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTT------GGYMiytattfKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPN 431
Cdd:cd05973 94 MFTSGTTGLPKGVPVPLralaafGAYL-------RDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEGGFS 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 432 YPDpgrCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRhSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQ 511
Cdd:cd05973 167 VES---TWRVIERLGVTNLAGSPTAYRLLMAAGAEVPAR-PKGRLRRVSSAGEPLTPEVIRWFDAALG---VPIHDHYGQ 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 512 TETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGS-----WpgafrtlFGDHERYETTyfK 586
Cdd:cd05973 240 TELGMVLANHHALEHPVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIDIAnsplmW-------FRGYQLPDTP--A 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 587 PFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVP 666
Cdd:cd05973 311 IDGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHE 390
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 378548265 667 YSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05973 391 GTPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
199-712 |
1.32e-74 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 248.50 E-value: 1.32e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcna 278
Cdd:cd05971 8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT--- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 279 vkrgpktinlkaivdaaldqsskDGvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdAEDPLFLL 358
Cdd:cd05971 85 -----------------------DG-----------------------------------------------SDDPALII 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 359 YTSGSTGKPKGVLHTTGgYMIYTATTFKYAFDY--KSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNypDPG 436
Cdd:cd05971 95 YTSGTTGPPKGALHAHR-VLLGHLPGVQFPFNLfpRDGDLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRMTKF--DPK 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 437 RCWDIVDKYKVSIFYTAPTLVRsLMRDDDKfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDsrcPISDTWWQTEtGG 516
Cdd:cd05971 172 AALDLMSRYGVTTAFLPPTALK-MMRQQGE-QLKHAQVKLRAIATGGESLGEELLGWAREQFGV---EVNEFYGQTE-CN 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 517 FMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYEttyfKPFAGYYF-SG 595
Cdd:cd05971 246 LVIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDPVAFLGYWNNPSATE----KKMAGDWLlTG 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 596 DGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSL 675
Cdd:cd05971 322 DLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDALAREI 401
|
490 500 510
....*....|....*....|....*....|....*..
gi 378548265 676 VLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05971 402 QELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
171-712 |
2.44e-67 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 230.72 E-value: 2.44e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVEAGLGDKTAIhwegneLGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHS 250
Cdd:cd05959 9 VDLNLNEGRGDKTAF------IDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 251 VVFAGFSADSLAQRIVDCKPNVILTCNAvkrgpktinLKAIVDAALDQSSKDGVSVGICltydnslattreNTKWQNGRD 330
Cdd:cd05959 83 PVNTLLTPDDYAYYLEDSRARVVVVSGE---------LAPVLAAALTKSEHTLVVLIVS------------GGAGPEAGA 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 331 VWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTF-KYAFDYKSTDVYWCTADCGWITGH 409
Cdd:cd05959 142 LLLAELVAAEAEQLKPAATHADDPAFWLYSSGSTGRPKGVVHLHAD-IYWTAELYaRNVLGIREDDVCFSAAKLFFAYGL 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 410 SYVTYGPMLNGATVVVFegaPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPINPS 489
Cdd:cd05959 221 GNSLTFPLSVGATTVLM---PERPTPAAVFKRIRRYRPTVFFGVPTLYAAMLAAPN--LPSRDLSSLRLCVSAGEALPAE 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 490 AWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpga 569
Cdd:cd05959 296 VGERWKARFG---LDILDGIGSTEMLHIFLSNRPGR--VRYGTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGP---- 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 570 fRTLFGDHERYETTYfKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEH 648
Cdd:cd05959 367 -SSATMYWNNRDKTR-DTFQGEWTrTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVED 444
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 649 EVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05959 445 EDGLTKPKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
181-712 |
1.24e-65 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 226.99 E-value: 1.24e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWeGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd05970 32 DKLALVW-CDDAGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPATHQLTAKD 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILtCNAVKRGPKTINlKAIVDAALDQ---SSKDGVSVGIClTYDNSLATTREntkwqngrdvwwqdvI 337
Cdd:cd05970 111 IVYRIESADIKMIV-AIAEDNIPEEIE-KAAPECPSKPklvWVGDPVPEGWI-DFRKLIKNASP---------------D 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 338 SQYPTSCEveWVDAEDPLFLLYTSGSTGKPKGVLHTTGgYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPM 417
Cdd:cd05970 173 FERPTANS--YPCGEDILLVYFSSGTTGMPKMVEHDFT-YPLGHIVTAKYWQNVREGGLHLTVADTGWGKAVWGKIYGQW 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVVFEGapNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRHSRKSLRVLGSVGEPINPSAWRWFFNV 497
Cdd:cd05970 250 IAGAAVFVYDY--DKFDPKALLEKLSKYGVTTFCAPPTIYRFLIRED---LSRYDLSSLRYCTTAGEALNPEVFNTFKEK 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 VGDSrcpISDTWWQTETGgFMITPLPGAWPqKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCV---KGSWPGAFRTLF 574
Cdd:cd05970 325 TGIK---LMEGFGQTETT-LTIATFPWMEP-KPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIrtsKGKPVGLFGGYY 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 GDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQG 654
Cdd:cd05970 400 KDAEKTAEVWHD---GYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQV 476
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 655 IYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05970 477 VKATIVLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIR 534
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
171-712 |
6.84e-63 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 217.82 E-value: 6.84e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVeAGLGDKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhs 250
Cdd:cd05936 5 LEEAA-RRFPDKTALIFMG------RKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAV-- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 251 VVfagfsadslaqrivdckP-NVILTcnavkrgpkTINLKAIVDaaldqssKDGVSVGICltydnslattrentkwqngr 329
Cdd:cd05936 76 VV-----------------PlNPLYT---------PRELEHILN-------DSGAKALIV-------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 330 DVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGG-YMIYTATTFKYAFDYKSTDVYWCTADCgwitg 408
Cdd:cd05936 103 AVSFTDLLAAGAPLGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRNlVANALQIKAWLEDLLEGDDVVLAALPL----- 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 409 hsYVTYG-------PMLNGATVVVFegaPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGS 481
Cdd:cd05936 178 --FHVFGltvalllPLALGATIVLI---PR-FRPIGVLKEIRKHRVTIFPGVPTMYIALLNAPE--FKKRDFSSLRLCIS 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 482 VGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGgfmitPL----PGAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GEc 556
Cdd:cd05936 250 GGAPLPVEVAERFEELTG---VPIVEGYGLTETS-----PVvavnPLDGPRKPGSIGIPLPGTEVKIVDDDGEELPpGE- 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGswPGAFRTLFGDHERYETTyFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:cd05936 321 VGELWVRG--PQVMKGYWNRPEETAEA-FV--DGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHP 395
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05936 396 AVAEAAVVGVPDPYSGEAVKAFVVLKEGASLTEE---EIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
168-712 |
9.76e-63 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 218.52 E-value: 9.76e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 168 YNCLDKNVEAgLGDKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKDNGVKKGDAVVIYL---PMLMElpiAMLACAR 244
Cdd:PRK06187 9 GRILRHGARK-HPDKEAVYFDGRRT------TYAELDERVNRLANALRALGVKKGDRVAVFDwnsHEYLE---AYFAVPK 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 245 IGAV-HSV-VFagFSADSLAQRIVDCKPNVILTcnavkrGPKTINLKAIVDAALDqsskdgvSVGICLTYDnslATTREN 322
Cdd:PRK06187 79 IGAVlHPInIR--LKPEEIAYILNDAEDRVVLV------DSEFVPLLAAILPQLP-------TVRTVIVEG---DGPAAP 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 323 TKWQNGRdvwWQDVIS-QYPTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHT----TGGYMIYTAttfkyAFDYKSTDVY 397
Cdd:PRK06187 141 LAPEVGE---YEELLAaASDTFDFPD-IDENDAAAMLYTSGTTGHPKGVVLShrnlFLHSLAVCA-----WLKLSRDDVY 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 398 -----------WctadcGWitghsyvTYGPMLNGATVVvfegapnYP---DPGRCWDIVDKYKVSIFYTAPTLVRSLMRD 463
Cdd:PRK06187 212 lvivpmfhvhaW-----GL-------PYLALMAGAKQV-------IPrrfDPENLLDLIETERVTFFFAVPTIWQMLLKA 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 464 ddKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGFM-ITPL----PGAWPqKPGSATFPFF 538
Cdd:PRK06187 273 --PRAYFVDFSSLRLVIYGGAALPPALLREFKEKFG---IDLVQGYGMTETSPVVsVLPPedqlPGQWT-KRRSAGRPLP 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 539 GVQPVIVDEKGNEIE--GECSGYLCVKGSWpgafRTL--FGDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDD 614
Cdd:PRK06187 347 GVEARIVDDDGDELPpdGGEVGEIIVRGPW----LMQgyWNRPEATAETIDG---GWLHTGDVGYIDEDGYLYITDRIKD 419
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 615 VINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSE-ELRKSLvlmvRNQIGAFAAPDRIH 693
Cdd:PRK06187 420 VIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDAkELRAFL----RGRLAKFKLPKRIA 495
|
570
....*....|....*....
gi 378548265 694 WAPGLPKTRSGKIMRRILR 712
Cdd:PRK06187 496 FVDELPRTSVGKILKRVLR 514
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
180-708 |
2.06e-62 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 215.55 E-value: 2.06e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:cd17631 9 PDRTALVFGGR------SLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILtcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisq 339
Cdd:cd17631 83 EVAYILADSGAKVLF----------------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 yptscevewvdaEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFkYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLN 419
Cdd:cd17631 98 ------------DDLALLMYTSGTTGRPKGAMLTHRNLLWNAVNAL-AALDLGPDDVLLVVAPLFHIGGLGVFTLPTLLR 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVFEGapnyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPINPSAWR--WFFNV 497
Cdd:cd17631 165 GGTVVILRK----FDPETVLDLIERHRVTSFFLVPTMIQALLQHPRFATTDLS--SLRAVIYGGAPMPERLLRalQARGV 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 VgdsrcpISDTWWQTETGGfMITPLPGAWPQ-KPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGD 576
Cdd:cd17631 239 K------FVQGYGMTETSP-GVTFLSPEDHRrKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRG--PHVMAGYWNR 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 577 HERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIY 656
Cdd:cd17631 310 PEATAAAFRD---GWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVV 386
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 378548265 657 AFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17631 387 AVVVPRPGAELDED---ELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
195-712 |
2.20e-61 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 212.71 E-value: 2.20e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd05919 8 DRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVV 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDP 354
Cdd:cd05919 88 T--------------------------------------------------------------------------SADDI 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADC--GWITGHSyvTYGPMLNGATVVVFEGAPny 432
Cdd:cd05919 94 AYLLYSSGTTGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMffGYGLGNS--LWFPLAVGASAVLNPGWP-- 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 433 pDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQT 512
Cdd:cd05919 170 -TAERVLATLARFRPTVLYGVPTFYANLLDSCA--GSPDALRSLRLCVSAGEALPRGLGERWMEHFG---GPILDGIGAT 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 513 ETGGFMITPLPGAWpqKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETTYFkpfAGYY 592
Cdd:cd05919 244 EVGHIFLSNRPGAW--RLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRG--PSAAVGYWNNPEKSRATFN---GGWY 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELR 672
Cdd:cd05919 317 RTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLA 396
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 378548265 673 KSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05919 397 RDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
181-713 |
1.12e-60 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 213.26 E-value: 1.12e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK08316 26 DKTALVFG------DRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAvkrgpktinLKAIVDAALDQSSKDGVsvgiclTYDNSLATTRENTKWQNGRDvwWQDviSQY 340
Cdd:PRK08316 100 LAYILDHSGARAFLVDPA---------LAPTAEAALALLPVDTL------ILSLVLGGREAPGGWLDFAD--WAE--AGS 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMI-YTATTFkyAFDYKSTDV-------YWCTAdcgwitghSYV 412
Cdd:PRK08316 161 VAEPDVE-LADDDLAQILYTSGTESLPKGAMLTHRALIAeYVSCIV--AGDMSADDIplhalplYHCAQ--------LDV 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 413 TYGPMLN-GATVVVFEGapnyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkFVTRHSRkSLR------------VL 479
Cdd:PRK08316 230 FLGPYLYvGATNVILDA----PDPELILRTIEAERITSFFAPPTVWISLLRHPD-FDTRDLS-SLRkgyygasimpveVL 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 GSVGEPInPSAWrwFFNVVGdsrcpisdtwwQTEtggfmITPL-----PGAWPQKPGSATFPFFGVQPVIVDEKGNEI-E 553
Cdd:PRK08316 304 KELRERL-PGLR--FYNCYG-----------QTE-----IAPLatvlgPEEHLRRPGSAGRPVLNVETRVVDDDGNDVaP 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 554 GE-----------CSGYlcvkgswpgafrtlFGDHERYETTyfkpFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGH 621
Cdd:PRK08316 365 GEvgeivhrspqlMLGY--------------WDDPEKTAEA----FRGGWFhSGDLGVMDEEGYITVVDRKKDMIKTGGE 426
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 622 RIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKT 701
Cdd:PRK08316 427 NVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTED---ELIAHCRARLAGFKVPKRVIFVDELPRN 503
|
570
....*....|..
gi 378548265 702 RSGKIMRRILRK 713
Cdd:PRK08316 504 PSGKILKRELRE 515
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
187-713 |
3.07e-58 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 206.55 E-value: 3.07e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 187 WEGNELGVDASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRI 265
Cdd:cd05928 31 WWVNGKGDEVKWSFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRL 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 266 VDCKPNVILTCNAvkrgpktinLKAIVDAALDQsskdgvsvgiCLTYDNSLATTrentkwQNGRDVW--WQDVISQYPTS 343
Cdd:cd05928 111 QASKAKCIVTSDE---------LAPEVDSVASE----------CPSLKTKLLVS------EKSRDGWlnFKELLNEASTE 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 344 CEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATV 423
Cdd:cd05928 166 HHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQGACV 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEgAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRHSRKSLRVLGSVGEPINPSA---WRwffNVVGd 500
Cdd:cd05928 246 FVHH-LPRF-DPLVILKTLSSYPITTFCGAPTVYRMLVQQD---LSSYKFPSLQHCVTGGEPLNPEVlekWK---AQTG- 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 501 srCPISDTWWQTETGgfMITPLPGAWPQKPGS--ATFPFFGVQpvIVDEKGNEI----EGECSgyLCVKGSWP-GAFRTL 573
Cdd:cd05928 317 --LDIYEGYGQTETG--LICANFKGMKIKPGSmgKASPPYDVQ--IIDDNGNVLppgtEGDIG--IRVKPIRPfGLFSGY 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FGDHERYETTYFKPFagyYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:cd05928 389 VDNPEKTAATIRGDF---YLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGE 465
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 654 GIYAFVTLleGVPYS----EELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05928 466 VVKAFVVL--APQFLshdpEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
194-707 |
1.11e-53 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 192.81 E-value: 1.11e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 194 VDA----SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:cd05911 3 IDAdtgkELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISK 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAVkrgpktinlkaiVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQ--NGRDVWWQDVISQyptsceve 347
Cdd:cd05911 83 PKVIFTDPDG------------LEKVKEAAKELGPKDKIIVLDDKPDGVLSIEDLLSptLGEEDEDLPPPLK-------- 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 wVDAEDPLFLLYTSGSTGKPKGVL--HTTGGYMIYTATTFKYAfDYKSTDVYWCTADCGWITG-HSYVTYgpMLNGATVV 424
Cdd:cd05911 143 -DGKDDTAAILYSSGTTGLPKGVClsHRNLIANLSQVQTFLYG-NDGSNDVILGFLPLYHIYGlFTTLAS--LLNGATVI 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 425 VFegapNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPInpsaWRWFFNVVG--DSR 502
Cdd:cd05911 219 IM----PKFDSELFLDLIEKYKITFLYLVPPIAAALAKSPL--LDKYDLSSLRVILSGGAPL----SKELQELLAkrFPN 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 503 CPISDTWWQTETGGfMITPLPGaWPQKPGSATFPFFGVQPVIVDEKGNEIEGECS-GYLCVKGswPGAFRTLFGDHERYE 581
Cdd:cd05911 289 ATIKQGYGMTETGG-ILTVNPD-GDDKPGSVGRLLPNVEAKIVDDDGKDSLGPNEpGEICVRG--PQVMKGYYNNPEATK 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 582 TTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTL 661
Cdd:cd05911 365 ETFDE--DGWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVR 442
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 378548265 662 LEGvpySEELRKSLVLMVRNQIGAFAA-PDRIHWAPGLPKTRSGKIM 707
Cdd:cd05911 443 KPG---EKLTEKEVKDYVAKKVASYKQlRGGVVFVDEIPKSASGKIL 486
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
171-713 |
4.96e-52 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 188.89 E-value: 4.96e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVEAGLGDKTAIhwegnelgVD--ASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV 248
Cdd:TIGR02262 10 LDRNVVEGRGGKTAF--------IDdiSSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 249 HSVVFAGFSADSLAQRIVDCKPNVILTCN--------AVKRGPktiNLKAIVDAALDQSSKDGVSvgicltydNSLATTr 320
Cdd:TIGR02262 82 PVALNTLLTADDYAYMLEDSRARVVFVSGallpvikaALGKSP---HLEHRVVVGRPEAGEVQLA--------ELLATE- 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 321 entkwqngrdvwwQDVISQYPTScevewvdAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTF-KYAFDYKSTDVYWC 399
Cdd:TIGR02262 150 -------------SEQFKPAATQ-------ADDPAFWLYSSGSTGMPKGVVHTHSN-PYWTAELYaRNTLGIREDDVCFS 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 400 TADCGWITGHSYVTYGPMLNGATVVVFegaPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVL 479
Cdd:TIGR02262 209 AAKLFFAYGLGNALTFPMSVGATTVLM---GERPTPDAVFDRLRRHQPTIFYGVPTLYAAMLADPN--LPSEDQVRLRLC 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 GSVGEPINPSA---WRWFFNVvgdsrcPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:TIGR02262 284 TSAGEALPAEVgqrWQARFGV------DIVDGIGSTEMLHIFLSNLPGD--VRYGTSGKPVPGYRLRLVGDGGQDVADGE 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGswPGAFRTLFGDHERYETTYFKPFAGyyfSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:TIGR02262 356 PGELLISG--PSSATMYWNNRAKSRDTFQGEWTR---SGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHP 430
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGvpySEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:TIGR02262 431 AVLEAAVVGVADEDGLIKPKAFVVLRPG---QTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
195-713 |
2.03e-51 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 186.75 E-value: 2.03e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd05926 12 TPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVL 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCN-----AVKRGPKTinLKAIVDAALDqsskdgvsVGICLTYD--NSLATTRENTKWQNGRDVwwqdvisqyptsceve 347
Cdd:cd05926 92 TPKgelgpASRAASKL--GLAILELALD--------VGVLIRAPsaESLSNLLADKKNAKSEGV---------------- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 wVDAEDPLFLLYTSGSTGKPKGVLHTTGgyMIYTATTfKYAFDYKSTDvywctADCGWIT-----GHSYVT--YGPMLNG 420
Cdd:cd05926 146 -PLPDDLALILHTSGTTGRPKGVPLTHR--NLAASAT-NITNTYKLTP-----DDRTLVVmplfhVHGLVAslLSTLAAG 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVfegaPNYPDPGRCWDIVDKYKVSiFYTA-PTLVRSLMRDDDKfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVG 499
Cdd:cd05926 217 GSVVL----PPRFSASTFWPDVRDYNAT-WYTAvPTIHQILLNRPEP-NPESPPPKLRFIRSCSASLPPAVLEALEATFG 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dsrCPISDTWWQTETGGFMIT-PLPGAwPQKPGSATFPFfGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHE 578
Cdd:cd05926 291 ---APVLEAYGMTEAAHQMTSnPLPPG-PRKPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRG--PNVTRGYLNNPE 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 579 RYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAF 658
Cdd:cd05926 364 ANAEAAFK--DGWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAA 441
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 659 VTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05926 442 VVLREGASVTeEELRAFC----RKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
199-712 |
4.51e-51 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 184.03 E-value: 4.51e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcna 278
Cdd:cd05934 5 TYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 279 vkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaeDPLFLL 358
Cdd:cd05934 82 --------------------------------------------------------------------------DPASIL 87
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 359 YTSGSTGKPKGVLhTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVfegAPNYpDPGRC 438
Cdd:cd05934 88 YTSGTTGPPKGVV-ITHANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVL---LPRF-SASRF 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 439 WDIVDKYKVSIFYTAPTLVRSLM----RDDDkfvtRHSRksLRVLGsvGEPINPSAWRWFFNVVGdsrCPISDTWWQTET 514
Cdd:cd05934 163 WSDVRRYGATVTNYLGAMLSYLLaqppSPDD----RAHR--LRAAY--GAPNPPELHEEFEERFG---VRLLEGYGMTET 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGFMITPLPGawPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWP-GAFRTLFGDHEryETTyfKPFA-GYY 592
Cdd:cd05934 232 IVGVIGPRDE--PRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRGLRGwGFFKGYYNMPE--ATA--EAMRnGWF 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElr 672
Cdd:cd05934 306 HTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPE-- 383
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 378548265 673 kSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05934 384 -ELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
198-715 |
5.59e-51 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 183.92 E-value: 5.59e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRivdckpnviltcn 277
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDR------------- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 aVKRGPKTInlkAIVDaaldqsskdgvsvgicltydnslattrENTKwqngrdvwwqdvisqyptscevewvdAEDPLFL 357
Cdd:cd05974 68 -VDRGGAVY---AAVD---------------------------ENTH--------------------------ADDPMLL 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGYMIYTATTFkYAFDYKSTDVYWCTADCGWiTGHSYVT-YGPMLNGATVVVFegapNYP--D 434
Cdd:cd05974 91 YFTSGTTSKPKLVEHTHRSYPVGHLSTM-YWIGLKPGDVHWNISSPGW-AKHAWSCfFAPWNAGATVFLF----NYArfD 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 435 PGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRhSRKSLRVLGSVGEPINPSAWRWFFNVVGDSrcpISDTWWQTET 514
Cdd:cd05974 165 AKRVLAALVRYGVTTLCAPPTVWRMLIQQD---LAS-FDVKLREVVGAGEPLNPEVIEQVRRAWGLT---IRDGYGQTET 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGfMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNEI-EGECSgyLCVKGSWP-GAFRTLFGDHERyetTYFKPFAGYY 592
Cdd:cd05974 238 TA-LVGNSPGQ-PVKAGSMGRPLPGYRVALLDPDGAPAtEGEVA--LDLGDTRPvGLMKGYAGDPDK---TAHAMRGGYY 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELR 672
Cdd:cd05974 311 RTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPETA 390
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 378548265 673 KSLVLMVRNQIGAFAAPDRIHWAPgLPKTRSGKIMRRILRKIA 715
Cdd:cd05974 391 LEIFRFSRERLAPYKRIRRLEFAE-LPKTISGKIRRVELRRRE 432
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
195-712 |
5.53e-49 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 178.44 E-value: 5.53e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGV-KKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVI 273
Cdd:cd05958 8 EREWTYRDLLALANRIANVLVGELGiVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKARITVA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 274 LTCNAVkrgpktinlkaivdaaldqsskdgvsvgicltydnslaTTREntkwqngrdvwwqdvisqyptscevewvdaeD 353
Cdd:cd05958 88 LCAHAL--------------------------------------TASD-------------------------------D 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGApnyp 433
Cdd:cd05958 99 ICILAFTSGTTGAPKATMHFHRDPLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEA---- 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 434 DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTE 513
Cdd:cd05958 175 TPDLLLSAIARYKPTVLFTAPTAYRAMLAHPDA--AGPDLSSLRKCVSAGEALPAALHRAWKEATG---IPIIDGIGSTE 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 514 TGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERyetTYFKpfAGYYF 593
Cdd:cd05958 250 MFHIFISARPGD--ARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRG--PTGCRYLADKRQR---TYVQ--GGWNI 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 594 SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRK 673
Cdd:cd05958 321 TGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLAR 400
|
490 500 510
....*....|....*....|....*....|....*....
gi 378548265 674 SLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05958 401 ELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
181-709 |
2.17e-48 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 176.95 E-value: 2.17e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd05930 2 DAVAVVDGDQ------SLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd05930 76 LAYILEDSGAKLVLT----------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATtFKYAFDYKSTDVYWCTADCGWItGHSYVTYGPMLNG 420
Cdd:cd05930 91 ---------DPDDLAYVIYTSGSTGKPKGVMVEHRGLVNLLLW-MQEAYPLTPGDRVLQFTSFSFD-VSVWEIFGALLAG 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVfegAPN--YPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVtrhsRKSLRVLGSVGEPINPSAWR-WF--- 494
Cdd:cd05930 160 ATLVV---LPEevRKDPEALADLLAEEGITVLHLTPSLLRLLLQELELAA----LPSLRLVLVGGEALPPDLVRrWRell 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 -----FNVVGDSRCPISDTWWQTETGGFMITPLP-GAwpqkpgsatfPFFGVQPVIVDEKGNE----IEGEcsgyLCVKG 564
Cdd:cd05930 233 pgarlVNLYGPTEATVDATYYRVPPDDEEDGRVPiGR----------PIPNTRVYVLDENLRPvppgVPGE----LYIGG 298
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 565 swPGAFRtlfGDHERYETTYFK----PFAG---YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 637
Cdd:cd05930 299 --AGLAR---GYLNRPELTAERfvpnPFGPgerMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLAHPG 373
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 638 CAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRR 709
Cdd:cd05930 374 VREAAVVAREDGDGEKRLVAYVVPDEGGELDeEELRAHL----AERLPDYMVPSAFVVLDALPLTPNGKVDRK 442
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
197-713 |
6.80e-47 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 172.57 E-value: 6.80e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTc 276
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVV- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngRDVWWQDVISQYPtscevewvdaEDPLF 356
Cdd:cd05903 80 ----------------------------------------------------PERFRQFDPAAMP----------DAVAL 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYMiytATTFKYAFDYKST--DVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGApnypD 434
Cdd:cd05903 98 LLFTSGTTGEPKGVMHSHNTLS---ASIRQYAERLGLGpgDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIW----D 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 435 PGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRksLRVLGSVGEPINPSAWRWFFNVVGDSRCPIsdtWWQTET 514
Cdd:cd05903 171 PDKALALMREHGVTFMMGATPFLTDLLNAVEEAGEPLSR--LRTFVCGGATVPRSLARRAAELLGAKVCSA---YGSTEC 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFrtlFGDHERYETTYFKPFAGYYFS 594
Cdd:cd05903 246 PGAVTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRG--PSVF---LGYLDRPDLTADAAPEGWFRT 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 595 GDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEG-VPYSEELRK 673
Cdd:cd05903 321 GDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGaLLTFDELVA 400
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 378548265 674 SLvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05903 401 YL---DRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRLRE 437
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
179-713 |
4.14e-43 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 163.54 E-value: 4.14e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 179 LGDKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSA 258
Cdd:PRK07656 18 FGDKEAYVFGDQ------RLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYTA 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 DSLAQRIVDCKPNVILTCNAVkrgpktinlkaivdAALDQSSKDGV----SVGICLTYDNSLATTRENTkwqngrdvwWQ 334
Cdd:PRK07656 92 DEAAYILARGDAKALFVLGLF--------------LGVDYSATTRLpaleHVVICETEEDDPHTEKMKT---------FT 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 335 DVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLhTTGGYMIYTATTFKYAFDYKSTDVYWC----------TAdcG 404
Cdd:PRK07656 149 DFLAAGDPAERAPEVDPDDVADILFTSGTTGRPKGAM-LTHRQLLSNAADWAEYLGLTEGDRYLAanpffhvfgyKA--G 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 405 WITghsyvtygPMLNGATVV---VFegapnypDPGRCWDIVDKYKVSIFYTAPTLVRSL----MRDDDKFvtrhsrKSLR 477
Cdd:PRK07656 226 VNA--------PLMRGATILplpVF-------DPDEVFRLIETERITVLPGPPTMYNSLlqhpDRSAEDL------SSLR 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 478 VLGSVGEPINPSAWRWF---FNVVgdsrcPISDTWWQTETGGFM-ITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIE 553
Cdd:PRK07656 285 LAVTGAASMPVALLERFeseLGVD-----IVLTGYGLSEASGVTtFNRLDDDRKTVAGTIGTAIAGVENKIVNELGEEVP 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 554 GECSGYLCVKGswPGAFRTLFGDHEryETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV 633
Cdd:PRK07656 360 VGEVGELLVRG--PNVMKGYYDDPE--ATAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLY 435
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 634 LHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK07656 436 EHPAVAEAAVIGVPDERLGEVGKAYVVLKPGAELTEE---ELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
180-715 |
1.13e-42 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 162.62 E-value: 1.13e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIhwegneLGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV-------H--- 249
Cdd:COG1021 39 PDRIAV------VDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIpvfalpaHrra 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 250 ----------------SVVFAGFSADSLAQRIVDCKPnviltcnavkrGPKTInlkaIVDAaldqSSKDGVSvgicltyd 313
Cdd:COG1021 113 eishfaeqseavayiiPDRHRGFDYRALARELQAEVP-----------SLRHV----LVVG----DAGEFTS-------- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 314 nslattrentkwqngrdvwWQDVISQyPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYmIYTATTFKYAFDYKS 393
Cdd:COG1021 166 -------------------LDALLAA-PADLSEPRPDPDDVAFFQLSGGTTGLPKLIPRTHDDY-LYSVRASAEICGLDA 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 394 TDVYWCTADcgwiTGHSY-----VTYGPMLNGATVVVfegAPNyPDPGRCWDIVDKYKVSIfyTA--PTLVRSLMRDDDK 466
Cdd:COG1021 225 DTVYLAALP----AAHNFplsspGVLGVLYAGGTVVL---APD-PSPDTAFPLIERERVTV--TAlvPPLALLWLDAAER 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 467 fvTRHSRKSLRVLGSVGEPINPSAWR--------WFFNVVG------------DSR--------CPIS--DTWWqtetgg 516
Cdd:COG1021 295 --SRYDLSSLRVLQVGGAKLSPELARrvrpalgcTLQQVFGmaeglvnytrldDPEevilttqgRPISpdDEVR------ 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 517 fmitplpgawpqkpgsatfpffgvqpvIVDEKGNEI-EGECsGYLCVKGswPGAFRTLFGDHErYETTYFKPfAGYYFSG 595
Cdd:COG1021 367 ---------------------------IVDEDGNPVpPGEV-GELLTRG--PYTIRGYYRAPE-HNARAFTP-DGFYRTG 414
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 596 DGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSL 675
Cdd:COG1021 415 DLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPRGEPLTLAELRRFL 494
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 378548265 676 vlmvRNQ-IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:COG1021 495 ----RERgLAAFKLPDRLEFVDALPLTAVGKIDKKALRAAL 531
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
199-643 |
6.25e-42 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 157.81 E-value: 6.25e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcn 277
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivDAALDQSSKDGVSVGICLTYDNSLATTRENTKwqngrdvwwqdvisqyPTSCEVewVDAEDPLFL 357
Cdd:TIGR01733 79 ---------------DSALASRLAGLVLPVILLDPLELAALDDAPAP----------------PPPDAP--SGPDDLAYV 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVywctadcgWITGHSYV-------TYGPMLNGATVVVFEGAP 430
Cdd:TIGR01733 126 IYTSGSTGRPKGVVVTHRS-LVNLLAWLARRYGLDPDDR--------VLQFASLSfdasveeIFGALLAGATLVVPPEDE 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 431 NYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhSRKSLRVLGSVGEPINPSA---WRWffnVVGDSRcpisd 507
Cdd:TIGR01733 197 ERDDAALLAALIAEHPVTVLNLTPSLLALLAAALPP-----ALASLRLVILGGEALTPALvdrWRA---RGPGAR----- 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 508 tWWQ----TE-TGGFMITPLPGAWPQKPGSATF--PFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERY 580
Cdd:TIGR01733 264 -LINlygpTEtTVWSTATLVDPDDAPRESPVPIgrPLANTRLYVLDDDLRPVPVGVVGELYIGG--PGVARGYLNRPELT 340
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 378548265 581 ETTYFK-PFAG-----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAV 643
Cdd:TIGR01733 341 AERFVPdPFAGgdgarLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
180-711 |
1.22e-41 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 158.61 E-value: 1.22e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:cd12116 1 PDATAVRDD------DRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPAD 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTcnavkrgpktinlkaivdaalDQSSKDGVSVGICLTYDNSLATTRentkwqngrdvwwqDVISQ 339
Cdd:cd12116 75 RLRYILEDAEPALVLT---------------------DDALPDRLPAGLPVLLLALAAAAA--------------APAAP 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 YPTscevewVDAEDPLFLLYTSGSTGKPKGV-----------------LHTTGGYMIYTATTfkYAFDYKSTDVYWctad 402
Cdd:cd12116 120 RTP------VSPDDLAYVIYTSGSTGRPKGVvvshrnlvnflhsmrerLGLGPGDRLLAVTT--YAFDISLLELLL---- 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 403 cgwitghsyvtygPMLNGATVVVFEGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhSRKSLRVL-Gs 481
Cdd:cd12116 188 -------------PLLAGARVVIAPRETQR-DPEALARLIEAHSITVMQATPATWRMLLDAGWQ-----GRAGLTALcG- 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 482 vGEPINP-----------SAWrwffNVVGdsrcPISDTWWQTetggfmITPLPGAWPQKPgsATFPFFGVQPVIVDEKGN 550
Cdd:cd12116 248 -GEALPPdlaarllsrvgSLW----NLYG----PTETTIWST------AARVTAAAGPIP--IGRPLANTQVYVLDAALR 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 551 EI-EGECsGYLCVKGswPGAFRtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGH 621
Cdd:cd12116 311 PVpPGVP-GELYIGG--DGVAQ---GYLGRPALTAERfvpdPFAGpgsrLYRTGDLVRRRADGRLEYLGRADGQVKIRGH 384
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 622 RIGTAEVESALVLHPQCAEAAVVgIEHEVKGQGIYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPK 700
Cdd:cd12116 385 RIELGEIEAALAAHPGVAQAAVV-VREDGGDRRLVAYVVLKAGAAPDaAALRAHL----RATLPAYMVPSAFVRLDALPL 459
|
570
....*....|.
gi 378548265 701 TRSGKIMRRIL 711
Cdd:cd12116 460 TANGKLDRKAL 470
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
96-728 |
5.04e-41 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 160.63 E-value: 5.04e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 96 KEFSGQALVSSPQQYMEMHKRSMDDPaafwsdiasEFYWKQKWGDQ--VFSENldvrkgPISI-----------EWFKGG 162
Cdd:PLN03052 108 KELLGSKYKDPISSFSEFQRFSVENP---------EVYWSIVLDELslVFSVP------PRCIldtsdesnpggQWLPGA 172
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 163 ITNICYNCLDKNVEAGLgDKTAIHW--EGNE-LGVDaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAM 239
Cdd:PLN03052 173 VLNVAECCLTPKPSKTD-DSIAIIWrdEGSDdLPVN-RMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIY 250
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 240 LACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKA-IVDAaldQSSK------DGVSVGICLty 312
Cdd:PLN03052 251 LAIILAGCVVVSIADSFAPSEIATRLKISKAKAIFTQDVIVRGGKSIPLYSrVVEA---KAPKaivlpaDGKSVRVKL-- 325
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 313 dnslattRENtkwqngrDVWWQDVISQY-PTSCEVEWVDAEDPL----FLLYTSGSTGKPKGVLHTtggymiyTATTFKY 387
Cdd:PLN03052 326 -------REG-------DMSWDDFLARAnGLRRPDEYKAVEQPVeaftNILFSSGTTGEPKAIPWT-------QLTPLRA 384
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 388 A------FDYKSTDVY-WCTaDCGWITGHsYVTYGPMLNGATVVVFEGAPNYPDPGRcwdIVDKYKVSIFYTAPTLVRS- 459
Cdd:PLN03052 385 AadawahLDIRKGDIVcWPT-NLGWMMGP-WLVYASLLNGATLALYNGSPLGRGFAK---FVQDAKVTMLGTVPSIVKTw 459
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 460 ----LMRDDDKFvtrhsrkSLRVLGSVGEPINPSAWRWFFnvvgdSRC---PISDTWWQTETGGFMITplpGAWPQKPGS 532
Cdd:PLN03052 460 kntnCMAGLDWS-------SIRCFGSTGEASSVDDYLWLM-----SRAgykPIIEYCGGTELGGGFVT---GSLLQPQAF 524
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 533 ATF--PFFGVQPVIVDEKGNEI--EGECSGYLCVKGSWPGAFRTLF-GDHERyetTYFK--P-FAGYYFS--GDGCSRDK 602
Cdd:PLN03052 525 AAFstPAMGCKLFILDDSGNPYpdDAPCTGELALFPLMFGASSTLLnADHYK---VYFKgmPvFNGKILRrhGDIFERTS 601
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 603 DGYYWLTGRVDDVINVSGHRIGTAEVESAL-VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS----EELRKSLVL 677
Cdd:PLN03052 602 GGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLETAAIGVPPPGGGPEQLVIAAVLKDPPGSnpdlNELKKIFNS 681
|
650 660 670 680 690
....*....|....*....|....*....|....*....|....*....|.
gi 378548265 678 MVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKiasrQLEELGDTSTL 728
Cdd:PLN03052 682 AIQKKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQ----QLAQELSRSKL 728
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
194-711 |
7.90e-41 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 157.01 E-value: 7.90e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 194 VDAS----LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:cd05904 25 IDAAtgraLTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSG 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAvkrgpktiNLKAIVDAALdqsskdgvSVGICLTYDNSLATTRENTKWQNgrdvwwqdvisqyPTSCEVEWV 349
Cdd:cd05904 105 AKLAFTTAE--------LAEKLASLAL--------PVVLLDSAEFDSLSFSDLLFEAD-------------EAEPPVVVI 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTA-TTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEG 428
Cdd:cd05904 156 KQDDVAALLYSSGTTGRSKGVMLTHRNLIAMVAqFVAGEGSNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVVMPR 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 429 ApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDsrCPISDT 508
Cdd:cd05904 236 F----DLEELLAAIERYKVTHLPVVPPIVLALVKSPI--VDKYDLSSLRQIMSGAAPLGKELIEAFRAKFPN--VDLGQG 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 509 WWQTE-TGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGEC-SGYLCVKGswPGAFRTLFGDHERYETTYFK 586
Cdd:cd05904 308 YGMTEsTGVVAMCFAPEKDRAKYGSVGRLVPNVEAKIVDPETGESLPPNqTGELWIRG--PSIMKGYLNNPEATAATIDK 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 587 pfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVP 666
Cdd:cd05904 386 --EGWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKPGSS 463
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 378548265 667 YSEELRKSLvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05904 464 LTEDEIMDF---VAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
197-735 |
1.51e-39 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 155.11 E-value: 1.51e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLAcariGAVHSVVFA---GFSADSLAQRIVDCKPNVI 273
Cdd:PRK07529 58 TWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWG----GEAAGIANPinpLLEPEQIAELLRAAGAKVL 133
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 274 LT-------------CNAVKRGPktiNLKAIVDAALdqsskdgvsvGICLTYDNSLATTRENTKWQNgRDVWWQDVISQY 340
Cdd:PRK07529 134 VTlgpfpgtdiwqkvAEVLAALP---ELRTVVEVDL----------ARYLPGPKRLAVPLIRRKAHA-RILDFDAELARQ 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PTSCEV--EWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGhSYVT-YGPM 417
Cdd:PRK07529 200 PGDRLFsgRPIGPDDVAAYFHTGGTTGMPKLAQHTHGN-EVANAWLGALLLGLGPGDTVFCGLPLFHVNA-LLVTgLAPL 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVvFEGAPNYPDPG---RCWDIVDKYKVSIFYTAPTLVRSLMrddDKFVTRHSRKSLRVLGSVGEPINPSAWRWF 494
Cdd:PRK07529 278 ARGAHVV-LATPQGYRGPGviaNFWKIVERYRINFLSGVPTVYAALL---QVPVDGHDISSLRYALCGAAPLPVEVFRRF 353
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 FNVVGdsrCPISDTWWQTE-TGGFMITPLPGawPQKPGSA--TFPFFGVQPVIVDEKGNEIEgECS----GYLCVKGswP 567
Cdd:PRK07529 354 EAATG---VRIVEGYGLTEaTCVSSVNPPDG--ERRIGSVglRLPYQRVRVVILDDAGRYLR-DCAvdevGVLCIAG--P 425
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 GAFRT-LFGDHER---YETTYFKpfagyyfSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAV 643
Cdd:PRK07529 426 NVFSGyLEAAHNKglwLEDGWLN-------TGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLRHPAVALAAA 498
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 644 VGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAA-PDRIHWAPGLPKTRSGKIMRRILRKIASRQ---- 718
Cdd:PRK07529 499 VGRPDAHAGELPVAYVQLKPGASATEA---ELLAFARDHIAERAAvPKHVRILDALPKTAVGKIFKPALRRDAIRRvlra 575
|
570
....*....|....*...
gi 378548265 719 -LEELGdtstlADPSVVD 735
Cdd:PRK07529 576 aLRDAG-----VEAEVVD 588
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
181-712 |
2.37e-39 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 152.11 E-value: 2.37e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd17651 10 DAPALVAEG------RRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAER 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdAALDQSSKDGVSVGICLTYDNSLATtrentkwqngrdvwwqdvisQY 340
Cdd:cd17651 84 LAFMLADAGPVLVLT------------------HPALAGELAVELVAVTLLDQPGAAA--------------------GA 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PTSCEVEwVDAEDPLFLLYTSGSTGKPKGVL------------HTTGGYMIYTATTFKYA---FDYKSTDVYwctadcgw 405
Cdd:cd17651 126 DAEPDPA-LDADDLAYVIYTSGSTGRPKGVVmphrslanlvawQARASSLGPGARTLQFAglgFDVSVQEIF-------- 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsyvtyGPMLNGATVVVfegAPNY--PDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVG 483
Cdd:cd17651 197 ---------STLCAGATLVL---PPEEvrTDPPALAAWLDEQRISRVFLPTVALRALAEHGRPLGVRLA--ALRYLLTGG 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EP-INPSAWRWFFNVVGDSRcpISDTWWQTET---GGFMITPLPGAWPQKPGSATfPFFGVQPVIVDEKGNEIEGECSGY 559
Cdd:cd17651 263 EQlVLTEDLREFCAGLPGLR--LHNHYGPTEThvvTALSLPGDPAAWPAPPPIGR-PIDNTRVYVLDAALRPVPPGVPGE 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 560 LCVKGswPGAFRTLFGD----HERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLH 635
Cdd:cd17651 340 LYIGG--AGLARGYLNRpeltAERFVPDPFVPGARMYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARH 417
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 636 PQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd17651 418 PGVREAVVLAREDRPGEKRLVAYVVGDPEAPVDaAELRAAL----ATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
195-711 |
2.54e-39 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 152.10 E-value: 2.54e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAGFSadslaQRIVDckpnviL 274
Cdd:cd05920 38 DRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAV--PVLALPS-----HRRSE------L 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCNAVKRGPKTInlkaIVDAALDQsskdgvsvgicltyDNSLATTREntkwqngrdvwwqdVISQYPtscevewvdaeDP 354
Cdd:cd05920 105 SAFCAHAEAVAY----IVPDRHAG--------------FDHRALARE--------------LAESIP-----------EV 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYmIYTATTfkyafdykSTDVYWCTAD----CGWITGHSYVTYGP-----MLNGATVVV 425
Cdd:cd05920 142 ALFLLSGGTTGTPKLIPRTHNDY-AYNVRA--------SAEVCGLDQDtvylAVLPAAHNFPLACPgvlgtLLAGGRVVL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 426 fegAPNyPDPGRCWDIVDKYKVsifyTAPTLVRSL-MRDDDKFVTRHSR-KSLRVLGSVGEPINPSAWRWFFNVVGdsrC 503
Cdd:cd05920 213 ---APD-PSPDAAFPLIEREGV----TVTALVPALvSLWLDAAASRRADlSSLRLLQVGGARLSPALARRVPPVLG---C 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTE-----------------TGGFMITPLPGAWpqkpgsatfpffgvqpvIVDEKGNEI-EGEcSGYLCVKGs 565
Cdd:cd05920 282 TLQQVFGMAEgllnytrlddpdeviihTQGRPMSPDDEIR-----------------VVDEEGNPVpPGE-EGELLTRG- 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 566 wPGAFRTLFgDHERYETTYFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:cd05920 343 -PYTIRGYY-RAPEHNARAFTP-DGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVA 419
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 646 IEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMvrnQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05920 420 MPDELLGERSCAFVVLRDPPPSAAQLRRFLRER---GLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
180-711 |
7.29e-39 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 150.81 E-value: 7.29e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:cd12117 11 PDAVAVVYG------DRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELPAE 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGicltydnslattrentkwqngrdvwwqdvisq 339
Cdd:cd12117 85 RLAFMLADAGAKVLLTDRSLAGRAGGLEVAVVIDEALDAGPAGNPAVP-------------------------------- 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 yptscevewVDAEDPLFLLYTSGSTGKPKGV---------LHTTGGYMIYTA-TTF----KYAFDYKSTDVYwctadcgw 405
Cdd:cd12117 133 ---------VSPDDLAYVMYTSGSTGRPKGVavthrgvvrLVKNTNYVTLGPdDRVlqtsPLAFDASTFEIW-------- 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsyvtyGPMLNGATVVVFEGAPnYPDPGRCWDIVDKYKVS-IFYTAPtLVRSLMRDD-DKFvtrhsrKSLRVLGSVG 483
Cdd:cd12117 196 ---------GALLNGARLVLAPKGT-LLDPDALGALIAEEGVTvLWLTAA-LFNQLADEDpECF------AGLRELLTGG 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EPINPSAWRWFFNVVGDSRcpISDTWWQTETGGF----MITPLPGAWPQKP-GSatfPFFGVQPVIVDEKGNEIEGECSG 558
Cdd:cd12117 259 EVVSPPHVRRVLAACPGLR--LVNGYGPTENTTFttshVVTELDEVAGSIPiGR---PIANTRVYVLDEDGRPVPPGVPG 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 559 YLCVKGSwpGAFRTLFGD----HERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVL 634
Cdd:cd12117 334 ELYVGGD--GLALGYLNRpaltAERFVADPFGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRA 411
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 635 HPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPySEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd12117 412 HPGVREAVVVVREDAGGDKRLVAYVVAEGALD-AAELRAFL----RERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
181-711 |
2.92e-37 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 147.03 E-value: 2.92e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKD-NGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:PRK08314 25 DKTAIVFYGRAI------SYRELLEEAERLAGYLQQeCGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREE 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILT----CNAVKRGPKTINLK-AIVDA---ALDQSSKDGVSVGicltydnsLATTRENTKWQNGRDV 331
Cdd:PRK08314 99 ELAHYVTDSGARVAIVgselAPKVAPAVGNLRLRhVIVAQysdYLPAEPEIAVPAW--------LRAEPPLQALAPGGVV 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 332 WWQDVISQYPTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSY 411
Cdd:PRK08314 171 AWKEALAAGLAPPPHT-AGPDDLAVLPYTSGTTGVPKGCMHTHRTVM-ANAVGSVLWSNSTPESVVLAVLPLFHVTGMVH 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 412 VTYGPMLNGATVVVF-----EGAPnypdpgrcwDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPI 486
Cdd:PRK08314 249 SMNAPIYAGATVVLMprwdrEAAA---------RLIERYRVTHWTNIPTMVVDFLASPG--LAERDLSSLRYIGGGGAAM 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 487 NPSAWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVD-EKGNEI-EGEcSGYLCVKG 564
Cdd:PRK08314 318 PEAVAERLKELTG---LDYVEGYGLTETMAQTHSNPPDR--PKLQCLGIPTFGVDARVIDpETLEELpPGE-VGEIVVHG 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 565 swPGAFRTLFGDHERYETTyFKPFAGYYF--SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:PRK08314 392 --PQVFKGYWNRPEATAEA-FIEIDGKRFfrTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEAC 468
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 378548265 643 VVGIEHEVKGQGIYAFVTL---LEGVPYSEELRKslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK08314 469 VIATPDPRRGETVKAVVVLrpeARGKTTEEEIIA----WAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQL 536
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
199-713 |
2.95e-37 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 146.62 E-value: 2.95e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIylpML------MELpiaMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNV 272
Cdd:cd12119 27 TYAEVAERARRLANALRRLGVKPGDRVAT---LAwnthrhLEL---YYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRV 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 ILTcnavkrgpkTINLKAIVDAALDQssKDGVSVGICLTYDNSLATTRENTKWQngrdvwWQDVISQYPTSCEVEWVDAE 352
Cdd:cd12119 101 VFV---------DRDFLPLLEAIAPR--LPTVEHVVVMTDDAAMPEPAGVGVLA------YEELLAAESPEYDWPDFDEN 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGV--------LHTTGGYMiytattfKYAFDYKSTDVY-----------WCTADCGWITGHSYVT 413
Cdd:cd12119 164 TAAAICYTSGTTGNPKGVvyshrslvLHAMAALL-------TDGLGLSESDVVlpvvpmfhvnaWGLPYAAAMVGAKLVL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLNGATVVvfegapnypdpgrcwDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRW 493
Cdd:cd12119 237 PGPYLDPASLA---------------ELIEREGVTFAAGVPTVWQGLLDHLEA--NGRDLSSLRRVVIGGSAVPRSLIEA 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FfnvvGDSRCPISDTWWQTETG--GFMITPLPG----------AWPQKPGsatFPFFGVQPVIVDEKGNEIE--GECSGY 559
Cdd:cd12119 300 F----EERGVRVIHAWGMTETSplGTVARPPSEhsnlsedeqlALRAKQG---RPVPGVELRIVDDDGRELPwdGKAVGE 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 560 LCVKGSW-PGAFrtlFGDHERYEttyfKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 637
Cdd:cd12119 373 LQVRGPWvTKSY---YKNDEESE----ALTEDGWLrTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPA 445
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 638 CAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd12119 446 VAEAAVIGVPHPKWGERPLAVVVLKEGAtVTAEELLEFL----ADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALRE 518
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
353-713 |
1.06e-36 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 143.58 E-value: 1.06e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYW-CTAdcgWITGHSYVT--YGPMLNGATVVVFega 429
Cdd:cd05941 90 DPALILYTSGTTGRPKGVVLTHAN-LAANVRALVDAWRWTEDDVLLhVLP---LHHVHGLVNalLCPLFAGASVEFL--- 162
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 PNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRD------DDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDsrc 503
Cdd:cd05941 163 PKF-DPKEVAISRLMPSITVFMGVPTIYTRLLQYyeahftDPQFARAAAAERLRLMVSGSAALPVPTLEEWEAITGH--- 238
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTETGGFMITPLPGawPQKPGSATFPFFGVQPVIVDEKGNE-IEGECSGYLCVKGswPGAFRTLFGDHERYEt 582
Cdd:cd05941 239 TLLERYGMTEIGMALSNPLDG--ERRPGTVGMPLPGVQARIVDEETGEpLPRGEVGEIQVRG--PSVFKEYWNKPEATK- 313
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 583 tyfKPFA--GYYFSGDGCSRDKDGYYWLTGRV-DDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFV 659
Cdd:cd05941 314 ---EEFTddGWFKTGDLGVVDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVV 390
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 660 TLLEGVP--YSEELRKslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05941 391 VLRAGAAalSLEELKE----WAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
193-712 |
1.09e-36 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 144.83 E-value: 1.09e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 193 GVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNV 272
Cdd:PRK13391 20 STGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARA 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 ILTCNAvKRGpktinlkaIVDAALDQSSKdgvsVGICLTYDNSLATTRentkWQNgrdvwWQDVISQYPtscevEWVDAE 352
Cdd:PRK13391 100 LITSAA-KLD--------VARALLKQCPG----VRHRLVLDGDGELEG----FVG-----YAEAVAGLP-----ATPIAD 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPL--FLLYTSGSTGKPKGVLHTTGGYMIYTATT----FKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLnGATVVVF 426
Cdd:PRK13391 153 ESLgtDMLYSSGTTGRPKGIKRPLPEQPPDTPLPltafLQRLWGFRSDMVYLSPAPLYHSAPQRAVMLVIRL-GGTVIVM 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 EgapnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrcPIS 506
Cdd:PRK13391 232 E----HFDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKYDLSSLEVAIHAAAPCPPQVKEQMIDWWG----PII 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 507 DTWW-QTETGGFMITPlPGAWPQKPGSATFPFFGVqPVIVDEKGNEIEGECSGYLCVKGSWPgaFRTLFGDHERYETTYf 585
Cdd:PRK13391 304 HEYYaATEGLGFTACD-SEEWLAHPGTVGRAMFGD-LHILDDDGAELPPGEPGTIWFEGGRP--FEYLNDPAKTAEARH- 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 586 kPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV 665
Cdd:PRK13391 379 -PDGTWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGV 457
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 378548265 666 PYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK13391 458 DPGPALAAELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLR 504
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
171-705 |
1.68e-36 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 144.64 E-value: 1.68e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVEAgLGDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVH- 249
Cdd:PRK07798 9 FEAVADA-VPDRVALVCG------DRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPv 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 250 -----------------SVVFAGFSADSLAQRIVDCKPnviltcnavkRGPKtinLKAIVDAAlDQSSKDGVSVGIclTY 312
Cdd:PRK07798 82 nvnyryvedelryllddSDAVALVYEREFAPRVAEVLP----------RLPK---LRTLVVVE-DGSGNDLLPGAV--DY 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 313 DNSLATTRENtkwqngrdvwwQDVIsqyPTScevewvdaEDPLFLLYTSGSTGKPKGVLHTT--------GGYMIYTATT 384
Cdd:PRK07798 146 EDALAAGSPE-----------RDFG---ERS--------PDDLYLLYTGGTTGMPKGVMWRQedifrvllGGRDFATGEP 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 385 fkyafdykSTDVYWCTADCGWITG----------H---SYVTYGPMLNGATVVVfegapnYP----DPGRCWDIVDKYKV 447
Cdd:PRK07798 204 --------IEDEEELAKRAAAGPGmrrfpapplmHgagQWAAFAALFSGQTVVL------LPdvrfDADEVWRTIEREKV 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 448 -SIFYT----APTLVRSLMRDDDKFVTrhsrkSLRVLGSVGEPINPS---AW-RWFFNVVgdsrcpISDTWWQTETGgFM 518
Cdd:PRK07798 270 nVITIVgdamARPLLDALEARGPYDLS-----SLFAIASGGALFSPSvkeALlELLPNVV------LTDSIGSSETG-FG 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 519 ITPLPGAWPQKPGSATFPFfGVQPVIVDEKGNEIE--GECSGYLCVKGSWPGAFrtlFGDHERYETTyFKPFAG--YYFS 594
Cdd:PRK07798 338 GSGTVAKGAVHTGGPRFTI-GPRTVVLDEDGNPVEpgSGEIGWIARRGHIPLGY---YKDPEKTAET-FPTIDGvrYAIP 412
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 595 GDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkS 674
Cdd:PRK07798 413 GDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLA---E 489
|
570 580 590
....*....|....*....|....*....|.
gi 378548265 675 LVLMVRNQIGAFAAPDRIHWAPGLPKTRSGK 705
Cdd:PRK07798 490 LRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
181-719 |
2.22e-36 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 144.51 E-value: 2.22e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHwegNELGvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK06087 38 DKIAVV---DNHG--ASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAE 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAVKrgpKTINLKAIVDAALDQSSKDGVsVGIcltyDNSLATTRENTKWQngrdvwwqdVISQY 340
Cdd:PRK06087 113 LVWVLNKCQAKMFFAPTLFK---QTRPVDLILPLQNQLPQLQQI-VGV----DKLAPATSSLSLSQ---------IIADY 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNG 420
Cdd:PRK06087 176 EPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNN-ILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIG 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEgapnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPInPS-----AWRWff 495
Cdd:PRK06087 255 ARSVLLD----IFTPDACLALLEQQRCTCMLGATPFIYDLLNLLEKQPADLS--ALRFFLCGGTTI-PKkvareCQQR-- 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 496 nvvGDSRCPIsdtWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFG 575
Cdd:PRK06087 326 ---GIKLLSV---YGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRG--PNVFMGYLD 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHERyeTTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK06087 398 EPEL--TARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERS 475
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 656 YAFVTLLEGVpYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQL 719
Cdd:PRK06087 476 CAYVVLKAPH-HSLTLEEVVAFFSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRKDIMRRL 538
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
181-711 |
5.13e-36 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 141.68 E-value: 5.13e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd17643 2 EAVAVVDE------DRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd17643 76 IAFILADSGPSLLLT----------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvDAEDPLFLLYTSGSTGKPKGVLHTTGGYM-IYTATTfkYAFDYKSTDVywctadcgWITGHSYV------- 412
Cdd:cd17643 91 ---------DPDDLAYVIYTSGSTGRPKGVVVSHANVLaLFAATQ--RWFGFNEDDV--------WTLFHSYAfdfsvwe 151
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 413 TYGPMLNGATVVVfegAPNYP--DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGsvGEPINPSA 490
Cdd:cd17643 152 IWGALLHGGRLVV---VPYEVarSPEDFARLLRDEGVTVLNQTPSAFYQLVEAADRDGRDPLALRYVIFG--GEALEAAM 226
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 491 WRWFFNVVGDSRCPISDTWWQTETGGFM----ITP--LPGAWPQKPGsATFPFFGVQpvIVDEKGNEIEGECSGYLCVKG 564
Cdd:cd17643 227 LRPWAGRFGLDRPQLVNMYGITETTVHVtfrpLDAadLPAAAASPIG-RPLPGLRVY--VLDADGRPVPPGVVGELYVSG 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 565 swPGAFRtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:cd17643 304 --AGVAR---GYLGRPELTAERfvanPFGGpgsrMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHP 378
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17643 379 SVRDAAVIVREDEPGDTRLVAYVVADDGAaADIAELRALL----KELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
181-711 |
2.10e-35 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 139.76 E-value: 2.10e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd12115 14 DAIALVCG------DESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPER 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd12115 88 LRFILEDAQARLVLT----------------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvDAEDPLFLLYTSGSTGKPKGVLHTTGGymiytATTFkyafdykstdVYWCTADCG--WITGHSYVT----- 413
Cdd:cd12115 103 ---------DPDDLAYVIYTSGSTGRPKGVAIEHRN-----AAAF----------LQWAAAAFSaeELAGVLASTsicfd 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 ------YGPMLNGATVVVFEGAPNYPD-PGRCwdivdkyKVSIFYTAPTLVRSLMRDDdKFVTrhsrkSLRVLGSVGEPI 486
Cdd:cd12115 159 lsvfelFGPLATGGKVVLADNVLALPDlPAAA-------EVTLINTVPSAAAELLRHD-ALPA-----SVRVVNLAGEPL 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 487 N----------PSAWRwFFNVVGdsrcPISDTWWQTetgGFMITPLPGAWPqkpgSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:cd12115 226 PrdlvqrlyarLQVER-VVNLYG----PSEDTTYST---VAPVPPGASGEV----SIGRPLANTQAYVLDRALQPVPLGV 293
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGswPGAFRTLFGD----HERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 632
Cdd:cd12115 294 PGELYIGG--AGVARGYLGRpgltAERFLPDPFGPGARLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAAL 371
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 633 VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd12115 372 RSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAaGLVEDLRRHL----GTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
229-712 |
1.21e-34 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 138.41 E-value: 1.21e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 229 LPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVdaaldqsskdgVSVGI 308
Cdd:PLN03051 1 MPMTVDAVIIYLAIVLAGCVVVSVADSFSAKEIATRLDISGAKGVFTQDVVLRGGRALPLYSKV-----------VEAAP 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 309 CLTYDNSLATTRENTKWQNGrDVWWQDVI-SQYPTSC------EVEWVDAEDPLFLLYTSGSTGKPKGV--LHTTGgymI 379
Cdd:PLN03051 70 AKAIVLPAAGEPVAVPLREQ-DLSWCDFLgVAAAQGSvggneySPVYAPVESVTNILFSSGTTGEPKAIpwTHLSP---L 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 380 YTATTFKYAFDYKSTDVYWCTADCGWITGhSYVTYGPMLNGATVVVFEGAPNYPDPGrcwDIVDKYKVSIFYTAPTLVRS 459
Cdd:PLN03051 146 RCASDGWAHMDIQPGDVVCWPTNLGWMMG-PWLLYSAFLNGATLALYGGAPLGRGFG---KFVQDAGVTVLGLVPSIVKA 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 460 LMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRcPISDTWWQTETGGFMI--TPLPgawPQKPGSATFPF 537
Cdd:PLN03051 222 WRHTGAFAMEGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYK-PVIEYCGGTELASGYIssTLLQ---PQAPGAFSTAS 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 538 FGVQPVIVDEKGNEI--EGECSGYLCVKGSWPGAF-RTLFGDHERyetTYFKPFAGYYFS-------GDGCSRDKDGYYW 607
Cdd:PLN03051 298 LGTRFVLLNDNGVPYpdDQPCVGEVALAPPMLGASdRLLNADHDK---VYYKGMPMYGSKgmplrrhGDIMKRTPGGYFC 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 608 LTGRVDDVINVSGHRIGTAEVESALVLHPQC-AEAAVVGIEHEVKGQG---IYAFVTLLEGVPYS---EELRKSLVLMVR 680
Cdd:PLN03051 375 VQGRADDTMNLGGIKTSSVEIERACDRAVAGiAETAAVGVAPPDGGPEllvIFLVLGEEKKGFDQarpEALQKKFQEAIQ 454
|
490 500 510
....*....|....*....|....*....|..
gi 378548265 681 NQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PLN03051 455 TNLNPLFKVSRVKIVPELPRNASNKLLRRVLR 486
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
197-711 |
3.09e-34 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 136.07 E-value: 3.09e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCkpnviltc 276
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDS-------- 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navkrGPKTinlkAIVDAALDqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaeDPLF 356
Cdd:cd05935 73 -----GAKV----AVVGSELD-------------------------------------------------------DLAL 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVF-----EGAPn 431
Cdd:cd05935 89 IPYTSGTTGLPKGCMHTHFS-AAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMarwdrETAL- 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 432 ypdpgrcwDIVDKYKVSIFYTAPTLVRSLMrDDDKFVTRhSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQ 511
Cdd:cd05935 167 --------ELIEKYKVTFWTNIPTMLVDLL-ATPEFKTR-DLSSLKVLTGGGAPMPPAVAEKLLKLTG---LRFVEGYGL 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 512 TETggfmITPLPGAWPQKPGSATF--PFFGVQPVIVD-EKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETTYFKPF 588
Cdd:cd05935 234 TET----MSQTHTNPPLRPKLQCLgiP*FGVDARVIDiETGRELPPNEVGEIVVRG--PQIFKGYWNRPEETEESFIEIK 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 589 AGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV-- 665
Cdd:cd05935 308 GRRFFrTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYrg 387
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 378548265 666 PYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05935 388 KVTEE---DIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
181-715 |
3.14e-34 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 138.26 E-value: 3.14e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV----------HS 250
Cdd:PRK13295 39 DKTAVTAVRLGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVlnplmpifreRE 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 251 V----------------VFAGFSADSLAQRIVDCKPNviltcnavkrgpktinLKAIVdaALDQSSKDGvsvgicltYDN 314
Cdd:PRK13295 119 LsfmlkhaeskvlvvpkTFRGFDHAAMARRLRPELPA----------------LRHVV--VVGGDGADS--------FEA 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 315 SLATTRentkWQNGRDVwwqDVISQYPTScevewvDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiytATTFKYA--FDYK 392
Cdd:PRK13295 173 LLITPA----WEQEPDA---PAILARLRP------GPDDVTQLIYTSGTTGEPKGVMHTANTLM---ANIVPYAerLGLG 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 393 STDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEgapnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRdddkfVTRHS 472
Cdd:PRK13295 237 ADDVILMASPMAHQTGFMYGLMMPVMLGATAVLQD----IWDPARAAELIRTEGVTFTMASTPFLTDLTR-----AVKES 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 473 RK---SLRVLGSVGEPINPS----AWRwffnVVGDSRCPisdTWWQTETGGFMITpLPGAWPQKpGSAT--FPFFGVQPV 543
Cdd:PRK13295 308 GRpvsSLRTFLCAGAPIPGAlverARA----ALGAKIVS---AWGMTENGAVTLT-KLDDPDER-ASTTdgCPLPGVEVR 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 IVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETTyfkpFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRI 623
Cdd:PRK13295 379 VVDADGAPLPAGQIGRLQVRG--CSNFGGYLKRPQLNGTD----ADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENI 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 624 GTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFA-APDRIHWAPGLPKTR 702
Cdd:PRK13295 453 PVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFE---EMVEFLKAQKVAKQyIPERLVVRDALPRTP 529
|
570
....*....|...
gi 378548265 703 SGKIMRRILRKIA 715
Cdd:PRK13295 530 SGKIQKFRLREML 542
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
181-711 |
1.17e-33 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 134.68 E-value: 1.17e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd05945 6 DRPAVVEGG------RTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAER 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILtcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd05945 80 IREILDAAKPALLI------------------------------------------------------------------ 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYT---------------ATTFKYAFDYKSTDVYwctadCGW 405
Cdd:cd05945 94 --------ADGDDNAYIIFTSGSTGRPKGVQISHDNLVSFTnwmlsdfplgpgdvfLNQAPFSFDLSVMDLY-----PAL 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 ITGhsyvtygpmlnGATVVVFEGApnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEP 485
Cdd:cd05945 161 ASG-----------ATLVPVPRDA--TADPKQLFRFLAEHGITVWVSTPSFAAMCLLSPT--FTPESLPSLRHFLFCGEV 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 I-NPSAWRWFfnvvgdSR---CPISDTWWQTETggfMITPLPGAWPQKPGSAT------FPFFGVQPVIVDEKGNEIEGE 555
Cdd:cd05945 226 LpHKTARALQ------QRfpdARIYNTYGPTEA---TVAVTYIEVTPEVLDGYdrlpigYAKPGAKLVILDEDGRPVPPG 296
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 556 CSGYLCVKGswPGAFRTLFGDHERYETTYFkPFAGY--YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV 633
Cdd:cd05945 297 EKGELVISG--PSVSKGYLNNPEKTAAAFF-PDEGQraYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALR 373
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 634 LHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPY--SEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05945 374 QVPGVKEAVVVPKYKGEKVTELIAFVVPKPGAEAglTKAIKAEL----AERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
180-712 |
3.14e-33 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 134.81 E-value: 3.14e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGNElGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:PRK08008 21 GHKTALIFESSG-GVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLRE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTcnavkrGPKTINLKAIVDAALDQSSKdgvsvGICLTydnslattRENTKWQNGRDVWWQDVISQ 339
Cdd:PRK08008 100 ESAWILQNSQASLLVT------SAQFYPMYRQIQQEDATPLR-----HICLT--------RVALPADDGVSSFTQLKAQQ 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 YPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTT-----GGYmiYTAttFKYAFDYKstDVYW-----------CTADC 403
Cdd:PRK08008 161 PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHynlrfAGY--YSA--WQCALRDD--DVYLtvmpafhidcqCTAAM 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 404 gwitghsyvtygPMLN-GATVVVFEgapNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLM----RDDDKfvTRHSRKSLRV 478
Cdd:PRK08008 235 ------------AAFSaGATFVLLE---KY-SARAFWGQVCKYRATITECIPMMIRTLMvqppSANDR--QHCLREVMFY 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 479 LG-SVGEPinpSAWRWFFNVvgdsrcPISDTWWQTETGGFMITPLPGA---WPqkpgSATFPFFGVQPVIVDEKGNEIEG 554
Cdd:PRK08008 297 LNlSDQEK---DAFEERFGV------RLLTSYGMTETIVGIIGDRPGDkrrWP----SIGRPGFCYEAEIRDDHNRPLPA 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 555 ECSGYLCVKGSwPGafRTLF-GDHERYETT--YFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESA 631
Cdd:PRK08008 364 GEIGEICIKGV-PG--KTIFkEYYLDPKATakVLEA-DGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENI 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 632 LVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK08008 440 IATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEE---EFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
|
.
gi 378548265 712 R 712
Cdd:PRK08008 517 K 517
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
181-712 |
7.90e-33 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 133.57 E-value: 7.90e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLP----MLMELPIAMLACARIGAVHSVVfagf 256
Cdd:PRK06188 27 DRPALVLG------DTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLnrpeVLMAIGAAQLAGLRRTALHPLG---- 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SADSLAQRIVDCKPNViLTCNAVKRGPKTINLKAIVDaaldqSSKDGVSVGICltydnslattrentkwQNGRDVWwqDV 336
Cdd:PRK06188 97 SLDDHAYVLEDAGIST-LIVDPAPFVERALALLARVP-----SLKHVLTLGPV----------------PDGVDLL--AA 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTAttfkyafdykstdvyWCTADCGWITGHSYVTYGP 416
Cdd:PRK06188 153 AAKFGPAPLVAAALPPDIAGLAYTGGTTGKPKGVMGTHRSIATMAQ---------------IQLAEWEWPADPRFLMCTP 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 417 ------------MLNGATVVVFEGApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGE 484
Cdd:PRK06188 218 lshaggafflptLLRGGTVIVLAKF----DPAEVLRAIEEQRITATFLVPTMIYALLDHPD--LRTRDLSSLETVYYGAS 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 485 PINPSAWRWFFNVVGdsrcPI-SDTWWQTETGGFmITPLP-----GAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GEcS 557
Cdd:PRK06188 292 PMSPVRLAEAIERFG----PIfAQYYGQTEAPMV-ITYLRkrdhdPDDPKRLTSCGRPTPGLRVALLDEDGREVAqGE-V 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 558 GYLCVKGswPGAFRtlfGDHERYETTYfKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:PRK06188 366 GEICVRG--PLVMD---GYWNRPEETA-EAFRdGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHP 439
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK06188 440 AVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAA---ELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKALR 512
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
180-711 |
1.37e-32 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 132.40 E-value: 1.37e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:cd17646 12 PDAPAVVDEG------RTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYPAD 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTcnavkrgpktinlkaiVDAALDQSSKDGVSVGICLTYDNSLATTREntkwqngrdvwwqdvisq 339
Cdd:cd17646 86 RLAYMLADAGPAVVLT----------------TADLAARLPAGGDVALLGDEALAAPPATPP------------------ 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 yptscEVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATtFKYAFDYKSTDVYWCTADCG-----WitghsyVTY 414
Cdd:cd17646 132 -----LVP-PRPDNLAYVIYTSGSTGRPKGVMVTHAGIVNRLLW-MQDEYPLGPGDRVLQKTPLSfdvsvW------ELF 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 415 GPMLNGATVVVFEgAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfvtRHSRKSLRVLGSVGEPINPSAWRWF 494
Cdd:cd17646 199 WPLVAGARLVVAR-PGGHRDPAYLAALIREHGVTTCHFVPSMLRVFLAEPA----AGSCASLRRVFCSGEALPPELAARF 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 F--------NVVGDSRCPISDTWWQTEtggfmitplpGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGsw 566
Cdd:cd17646 274 LalpgaelhNLYGPTEAAIDVTHWPVR----------GPAETPSVPIGRPVPNTRLYVLDDALRPVPVGVPGELYLGG-- 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 567 PGAFRTLFG----DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:cd17646 342 VQLARGYLGrpalTAERFVPDPFGPGSRMYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAV 421
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 378548265 643 VVGIEHEVKGQGIYAFVTLLEG--VPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17646 422 VVARAAPAGAARLVGYVVPAAGaaGPDTAALRAHL----AERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
359-718 |
1.54e-32 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 129.52 E-value: 1.54e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 359 YTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGhSYVTYGPMLNGATVVVFEGAPNYPDPG-- 436
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSN-EVYNAWMLALNSLFDPDDVLLCGLPLFHVNG-SVVTLLTPLASGAHVVLAGPAGYRNPGlf 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 437 -RCWDIVDKYKVSIFYTAPTLVRSLM-RDDDKFVTrhsrkSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTET 514
Cdd:cd05944 87 dNFWKLVERYRITSLSTVPTVYAALLqVPVNADIS-----SLRFAMSGAAPLPVELRARFEDATG---LPVVEGYGLTEA 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGFMITPLPGAwPQKPGSA--TFPFFGVQPVIVDEKGN---EIEGECSGYLCVKGswPGAFR-TLFGDHERyettyfKPF 588
Cdd:cd05944 159 TCLVAVNPPDG-PKRPGSVglRLPYARVRIKVLDGVGRllrDCAPDEVGEICVAG--PGVFGgYLYTEGNK------NAF 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 589 A--GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVP 666
Cdd:cd05944 230 VadGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAV 309
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 378548265 667 YSEElrkSLVLMVRNQIGAFAA-PDRIHWAPGLPKTRSGKIMRRILRKIASRQ 718
Cdd:cd05944 310 VEEE---ELLAWARDHVPERAAvPKHIEVLEELPVTAVGKVFKPALRADAIHR 359
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
181-712 |
1.60e-32 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 131.34 E-value: 1.60e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsvvFAGFSADS 260
Cdd:cd17649 2 DAVALVFG------DQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGA----YVPLDPEY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIvdckpnviltcnavkrgpktinLKAIVDAaldqsskdGVSVgicltydnslattrentkwqngrdvwwqdVISQY 340
Cdd:cd17649 72 PAERL----------------------RYMLEDS--------GAGL-----------------------------LLTHH 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PtscevewvdaEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYaFDYKSTDVYWCTADCGWITGHSYVtYGPMLNG 420
Cdd:cd17649 93 P----------RQLAYVIYTSGSTGTPKGVAVSHGPLAAHCQATAER-YGLTPGDRELQFASFNFDGAHEQL-LPPLICG 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVfEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfVTRHSRKSLRVLGSVGEPINPSAWR-------W 493
Cdd:cd17649 161 ACVVL-RPDELWASADELAEMVRELGVTVLDLPPAYLQQLAEEADR-TGDGRPPSLRLYIFGGEALSPELLRrwlkapvR 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FFNVVGDSRCPISDTWWQTETGgfmitpLPGAWPQKP-GSatfPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRt 572
Cdd:cd17649 239 LFNAYGPTEATVTPLVWKCEAG------AARAGASMPiGR---PLGGRSAYILDADLNPVPVGVTGELYIGG--EGLAR- 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 573 lfGDHERYETT--------YFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVV 644
Cdd:cd17649 307 --GYLGRPELTaerfvpdpFGAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVV 384
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 645 GiEHEVKGQGIYAFVtLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd17649 385 A-LDGAGGKQLVAYV-VLRAAAAQPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
195-712 |
2.15e-32 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 131.95 E-value: 2.15e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK08276 9 GEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCNAvkrgpktinLKAIVDAALDQSSKDgvsVGICLTYDNSLATTRENTKWQNGrdvwwqdvisQYPTSCEVEWVDAEdp 354
Cdd:PRK08276 89 VSAA---------LADTAAELAAELPAG---VPLLLVVAGPVPGFRSYEEALAA----------QPDTPIADETAGAD-- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 lfLLYTSGSTGKPKGVL--------HTTGGYMiytATTFKYAFDYKSTDVYWCTADcgwiTGHSYVT-YGPMLN--GATV 423
Cdd:PRK08276 145 --MLYSSGTTGRPKGIKrplpgldpDEAPGMM---LALLGFGMYGGPDSVYLSPAP----LYHTAPLrFGMSALalGGTV 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEGApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPinpsawrwffnvvgdsrC 503
Cdd:PRK08276 216 VVMEKF----DAEEALALIERYRVTHSQLVPTMFVRMLKLPEEVRARYDVSSLRVAIHAAAP-----------------C 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PIS------DtWW---------QTETGGF-MITPlpGAWPQKPGSATFPFFGVqpV-IVDEKGNEI----EG----ECSG 558
Cdd:PRK08276 275 PVEvkramiD-WWgpiiheyyaSSEGGGVtVITS--EDWLAHPGSVGKAVLGE--VrILDEDGNELppgeIGtvyfEMDG 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 559 YlcvkgswpgAFrTLFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQC 638
Cdd:PRK08276 350 Y---------PF-EYHNDPEKTAAARNP--HGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKV 417
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 639 AEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK08276 418 ADVAVFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLR 491
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
181-743 |
3.13e-32 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 134.60 E-value: 3.13e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:COG1020 491 DAVAVVFG------DQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAER 564
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivDAALDQS-SKDGVSVgICLtyDnslattrentkwqngrdvwwQDVISQ 339
Cdd:COG1020 565 LAYMLEDAGARLVLT-----------------QSALAARlPELGVPV-LAL--D--------------------ALALAA 604
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 YPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVywctadCGWITGHS-----YVTY 414
Cdd:COG1020 605 EPATNPPVPVTPDDLAYVIYTSGSTGRPKGVMVEHRA-LVNLLAWMQRRYGLGPGDR------VLQFASLSfdasvWEIF 677
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 415 GPMLNGATVVVF--EGApnyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfvtRHSRKSLRVLGSVGEPINPSAWR 492
Cdd:COG1020 678 GALLSGATLVLAppEAR---RDPAALAELLARHRVTVLNLTPSLLRALLDAA-----PEALPSLRLVLVGGEALPPELVR 749
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 493 WFFNVVGDSRC-----PisdtwwqTETG----GFMITPLPGAWPQKP-GSatfPFFGVQPVIVDEKGNE----IEGEcsg 558
Cdd:COG1020 750 RWRARLPGARLvnlygP-------TETTvdstYYEVTPPDADGGSVPiGR---PIANTRVYVLDAHLQPvpvgVPGE--- 816
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 559 yLCVKGswPGafrtL----FGDH----ERYETTYFkPFAG--YYFSGDGCSRDKDG---YywlTGRVDDVINVSGHRIGT 625
Cdd:COG1020 817 -LYIGG--AG----LargyLNRPeltaERFVADPF-GFPGarLYRTGDLARWLPDGnleF---LGRADDQVKIRGFRIEL 885
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 626 AEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIgafAAPDRIHWAPGLPKTRSGK 705
Cdd:COG1020 886 GEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPY---MVPAAVVLLLPLPLTGNGK 962
|
570 580 590
....*....|....*....|....*....|....*...
gi 378548265 706 IMRRILRKIASRQLEELGDTSTLADPSVVDQLIALADV 743
Cdd:COG1020 963 LDRLALPAPAAAAAAAAAAPPAEEEEEEAALALLLLLV 1000
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
179-724 |
3.22e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 131.70 E-value: 3.22e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 179 LGDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHsvvfagfsa 258
Cdd:PRK07470 20 FPDRIALVWG------DRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVW--------- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 dslaqrivdCKPNVILTCNAVkrgpktinlkaivdAALDQSSkdGVSVGICLT----YDNSLATTRENTKWQ-------N 327
Cdd:PRK07470 85 ---------VPTNFRQTPDEV--------------AYLAEAS--GARAMICHAdfpeHAAAVRAASPDLTHVvaiggarA 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 328 GRDVwwQDVISQYP-TSCEVEWVDAEDPLFLLYTSGSTGKPK-GVLhtTGGYMIYTATTfkyafdyKSTDVYWCT--ADC 403
Cdd:PRK07470 140 GLDY--EALVARHLgARVANAAVDHDDPCWFFFTSGTTGRPKaAVL--THGQMAFVITN-------HLADLMPGTteQDA 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 404 gwitghSYVTyGPMLNGATV-----------VVFEGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHS 472
Cdd:PRK07470 209 ------SLVV-APLSHGAGIhqlcqvargaaTVLLPSERF-DPAEVWALVERHRVTNLFTVPTILKMLVEHPA--VDRYD 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 473 RKSLRVLGSVGEPI----NPSAWRWFFNVV----GDSRCpisdtwwqteTGGfmITPLPGAW------PQ-KPGSATFPF 537
Cdd:PRK07470 279 HSSLRYVIYAGAPMyradQKRALAKLGKVLvqyfGLGEV----------TGN--ITVLPPALhdaedgPDaRIGTCGFER 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 538 FGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYEttyfKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVI 616
Cdd:PRK07470 347 TGMEVQIQDDEGRELPPGETGEICVIG--PAVFAGYYNNPEANA----KAFRDGWFrTGDLGHLDARGFLYITGRASDMY 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 617 NVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAP 696
Cdd:PRK07470 421 ISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEA---ELLAWLDGKVARYKLPKRFFFWD 497
|
570 580
....*....|....*....|....*...
gi 378548265 697 GLPKTRSGKIMRRILRKiasrQLEELGD 724
Cdd:PRK07470 498 ALPKSGYGKITKKMVRE----ELEERGL 521
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
181-706 |
5.41e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 128.62 E-value: 5.41e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK06178 48 QRPAIIFYGHVI------TYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHE 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAvkrgpktinLKAIVDAALDQSSKDGV---SVGICLTYDNSL---ATTRENTKWQNGrdvwWQ 334
Cdd:PRK06178 122 LSYELNDAGAEVLLALDQ---------LAPVVEQVRAETSLRHVivtSLADVLPAEPTLplpDSLRAPRLAAAG----AI 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 335 DVISQY---PTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGgYMIYTATTF-KYAFDYKSTDVYWCTADCGWITGHS 410
Cdd:PRK06178 189 DLLPALracTAPVPLPPPALDALAALNYTGGTTGMPKGCEHTQR-DMVYTAAAAyAVAVVGGEDSVFLSFLPEFWIAGEN 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 YVTYGPMLNGATVVVFegapNYPDPGRCWDIVDKYKVSIfytAPTLVRS---LMrdDDKFVTRHSRKSLRVLGSVG--EP 485
Cdd:PRK06178 268 FGLLFPLFSGATLVLL----ARWDAVAFMAAVERYRVTR---TVMLVDNaveLM--DHPRFAEYDLSSLRQVRVVSfvKK 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPsawrwffnvvgDSRcpisdTWWQTETGGFMITplpGAW--PQKPGSATF--------------PFFGVQPV------ 543
Cdd:PRK06178 339 LNP-----------DYR-----QRWRALTGSVLAE---AAWgmTETHTCDTFtagfqdddfdllsqPVFVGLPVpgtefk 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 IVDEKGNEI-----EGEcsgyLCV------KGSW--PGAFRTLFGDheryettyfkpfaGYYFSGDGCSRDKDGYYWLTG 610
Cdd:PRK06178 400 ICDFETGELlplgaEGE----IVVrtpsllKGYWnkPEATAEALRD-------------GWLHTGDIGKIDEQGFLHYLG 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 611 RVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPD 690
Cdd:PRK06178 463 RRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAA---ALQAWCRENMAVYKVPE 539
|
570
....*....|....*.
gi 378548265 691 rIHWAPGLPKTRSGKI 706
Cdd:PRK06178 540 -IRIVDALPMTATGKV 554
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
198-711 |
9.40e-31 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 126.85 E-value: 9.40e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIvdckpnviltcn 277
Cdd:cd05923 29 LTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAELAELI------------ 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avKRGPKTINLKAiVDAALDQSSKDGvsvGICLTYDNSLATTRENTKWQngrdvwwqDVISqyPTSCEVEwvdaeDPLFL 357
Cdd:cd05923 97 --ERGEMTAAVIA-VDAQVMDAIFQS---GVRVLALSDLVGLGEPESAG--------PLIE--DPPREPE-----QPAFV 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVL---HTTGGYMIYTATTFKYAFDykSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVfegaPNYPD 434
Cdd:cd05923 156 FYTSGTTGLPKGAVipqRAAESRVLFMSTQAGLRHG--RHNVVLGLMPLYHVIGFFAVLVAALALDGTYVV----VEEFD 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 435 PGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPINPSAWRWFFNVVGDsrcPISDTWWQTET 514
Cdd:cd05923 230 PADALKLIEQERVTSLFATPTHLDALAAAAEFAGLKLS--SLRHVTFAGATMPDAVLERVNQHLPG---EKVNIYGTTEA 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGFMITPLPgawpqKPGSATFPFFG--VQPVIVDEKGNEI-----EGEcsgyLCVKGSWPGAFRtlfGDHERYETTYFKP 587
Cdd:cd05923 305 MNSLYMRDA-----RTGTEMRPGFFseVRIVRIGGSPDEAlangeEGE----LIVAAAADAAFT---GYLNQPEATAKKL 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPY 667
Cdd:cd05923 373 QDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREGTLS 452
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 378548265 668 SEELRKslvLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05923 453 ADELDQ---FCRASELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
198-714 |
2.08e-30 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 126.41 E-value: 2.08e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIltcn 277
Cdd:PRK06155 47 WTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHILRNSGARLL---- 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaIVDAALdQSSKDGVSVGicltyDNSLATTrentkWQNGRDVWWQ-----DVISQYPTSCEVEWVDAE 352
Cdd:PRK06155 123 -------------VVEAAL-LAALEAADPG-----DLPLPAV-----WLLDAPASVSvpagwSTAPLPPLDAPAPAAAVQ 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 --DPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTfkyafdykstdvywcTADCGWITGHSYVTYGP-------------M 417
Cdd:PRK06155 179 pgDTAAILYTSGTTGPSKGVCCPHAQFYWWGRNS---------------AEDLEIGADDVLYTTLPlfhtnalnaffqaL 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVV---FEGApnypdpgRCWDIVDKYKVSIFY----TAPTLVRSLMRDDDKfvtRHSrksLRV-LGSVGEPINPS 489
Cdd:PRK06155 244 LAGATYVLeprFSAS-------GFWPAVRRHGATVTYllgaMVSILLSQPARESDR---AHR---VRVaLGPGVPAALHA 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 490 AWRWFFNVvgdsrcPISDTWWQTETGGFMITPLPGawpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGA 569
Cdd:PRK06155 311 AFRERFGV------DLLDGYGSTETNFVIAVTHGS---QRPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADEPFA 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 570 FRT-LFGDHEryETTyfKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:PRK06155 382 FATgYFGMPE--KTV--EAWRNLWFhTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVP 457
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKI 714
Cdd:PRK06155 458 SELGEDEVMAAVVLRDGTALEPV---ALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQ 521
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
206-712 |
3.84e-30 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 124.47 E-value: 3.84e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 206 RVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQ--RIV--DCKPNViltcnavkr 281
Cdd:cd05922 2 GVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFVPLNPTLKESvlRYLvaDAGGRI--------- 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 282 gpktinlkAIVDAALDQSSKDGVSVgiclTYDNSLATtrentkwqnGRDVWWQDvisqyPTSCEVEWVDAEDPLFLLYTS 361
Cdd:cd05922 73 --------VLADAGAADRLRDALPA----SPDPGTVL---------DADGIRAA-----RASAPAHEVSHEDLALLLYTS 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 362 GSTGKPKGVL--HTTggyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGpMLNGATVVVfegAPNYPDPGRCW 439
Cdd:cd05922 127 GSTGSPKLVRlsHQN---LLANARSIAEYLGITADDRALTVLPLSYDYGLSVLNTH-LLRGATLVL---TNDGVLDDAFW 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 440 DIVDKYKVSIFYTAPT---LVRSLMRDDDKFvtrhsrKSLRVLGSVGEPINPSAWRWFFNVVGDSRcpISDTWWQTETGG 516
Cdd:cd05922 200 EDLREHGATGLAGVPStyaMLTRLGFDPAKL------PSLRYLTQAGGRLPQETIARLRELLPGAQ--VYVMYGQTEATR 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 517 FMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGD--HERYETTyfkpFAGYYFS 594
Cdd:cd05922 272 RMTYLPPERILEKPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVHRG--PNVMKGYWNDppYRRKEGR----GGGVLHT 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 595 GDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVkGQGIYAFVTLLEGVPYSEELRKs 674
Cdd:cd05922 346 GDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDPL-GEKLALFVTAPDKIDPKDVLRS- 423
|
490 500 510
....*....|....*....|....*....|....*...
gi 378548265 675 lvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05922 424 ----LAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
181-714 |
5.27e-30 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 124.59 E-value: 5.27e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIhwegneLGVDASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVhSVVFagfsad 259
Cdd:PRK06839 17 DRIAI------ITEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECI-AVPL------ 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 slaqrivdckpNVILTcnavkrgpktinlkaivDAALDQSSKD-GVSVGIC-LTYDNSLATTRENTKWQngRDVWWQDV- 336
Cdd:PRK06839 84 -----------NIRLT-----------------ENELIFQLKDsGTTVLFVeKTFQNMALSMQKVSYVQ--RVISITSLk 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 -ISQYPTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYG 415
Cdd:PRK06839 134 eIEDRKIDNFVE-KNESASFIICYTSGTTGKPKGAVLTQEN-MFWNALNNTFAIDLTMHDRSIVLLPLFHIGGIGLFAFP 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 416 PMLNGATVVVfegaPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTrhsrkslrvlgsvgepiNPSAWRWFF 495
Cdd:PRK06839 212 TLFAGGVIIV----PRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETT-----------------NLQSVRWFY 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 496 NvvGDSRCPI-------------SDTWWQTETGG--FMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYL 560
Cdd:PRK06839 271 N--GGAPCPEelmrefidrgflfGQGFGMTETSPtvFMLSEEDAR--RKVGSIGKPVLFCDYELIDENKNKVEVGEVGEL 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 561 CVKGswPGAFRTLFGDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAE 640
Cdd:PRK06839 347 LIRG--PNVMKEYWNRPDATEETIQD---GWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYE 421
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 641 AAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKI 714
Cdd:PRK06839 422 VAVVGRQHVKWGEIPIAFIVKKSSSVLIEK---DVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQ 492
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
199-712 |
7.56e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 124.15 E-value: 7.56e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcna 278
Cdd:PRK09088 24 TYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLLLG--- 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 279 vkrgpktinlkaivDAALDQSSKDGVSVgicltydnslattrentkwqngrDVWWQDVISQYPTscEVEWVDAEDPLFLL 358
Cdd:PRK09088 101 --------------DDAVAAGRTDVEDL-----------------------AAFIASADALEPA--DTPSIPPERVSLIL 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 359 YTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGApnypDPGRC 438
Cdd:PRK09088 142 FTSGTSGQPKGVMLSERN-LQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGF----EPKRT 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 439 --WDIVDKYKVSIFYTAPTLVRSLmRDDDKFVTRHSRkSLRVLGSVGEPINPSAWRWFFnvvgDSRCPISDTWWQTETGG 516
Cdd:PRK09088 217 lgRLGDPALGITHYFCVPQMAQAF-RAQPGFDAAALR-HLTALFTGGAPHAAEDILGWL----DDGIPMVDGFGMSEAGT 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 517 FMITPL-PGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHEryETTYFKPFAGYYFSG 595
Cdd:PRK09088 291 VFGMSVdCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRG--PNLSPGYWRRPQ--ATARAFTGDGWFRTG 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 596 DGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKS 674
Cdd:PRK09088 367 DIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDlERIRSH 446
|
490 500 510
....*....|....*....|....*....|....*...
gi 378548265 675 LVlmvrNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK09088 447 LS----TRLAKYKVPKHLRLVDALPRTASGKLQKARLR 480
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
190-712 |
2.20e-29 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 123.33 E-value: 2.20e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 190 NELGvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAvHSVVF-AGFSADSLAQRIVDC 268
Cdd:PRK13382 64 DELG---TLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGA-DILLLnTSFAGPALAEVVTRE 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 269 KPNVILTCNavkrgpktiNLKAIVDAALDQSSKdgvsvgicltydnslaTTReNTKWQNGRDVWWQDVISQYPTSCEVEW 348
Cdd:PRK13382 140 GVDTVIYDE---------EFSATVDRALADCPQ----------------ATR-IVAWTDEDHDLTVEVLIAAHAGQRPEP 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 349 VDAEDPLFLLyTSGSTGKPKGVLHT-TGGYMIYTATTFKYAfdykstdvywctadcgWITGHSYVTYGPMLN--GATVVV 425
Cdd:PRK13382 194 TGRKGRVILL-TSGTTGTPKGARRSgPGGIGTLKAILDRTP----------------WRAEEPTVIVAPMFHawGFSQLV 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 426 FEGAPNYP-------DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVV 498
Cdd:PRK13382 257 LAASLACTivtrrrfDPEATLDLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQF 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 GDSrcpISDTWWQTETGgfMI-TPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEI-EGECsGYLCVKGSwpgafrTLFgd 576
Cdd:PRK13382 337 GDV---IYNNYNATEAG--MIaTATPADLRAAPDTAGRPAEGTEIRILDQDFREVpTGEV-GTIFVRND------TQF-- 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 577 hERYETTYFKPF-AGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK13382 403 -DGYTSGSTKDFhDGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRL 481
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 656 YAFVTLLEGVPYSEELRKSlvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK13382 482 AAFVVLKPGASATPETLKQ---HVRDNLANYKVPRDIVVLDELPRGATGKILRRELQ 535
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
181-721 |
3.18e-29 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 123.22 E-value: 3.18e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK06710 39 EKKALHFLGKDI------TFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERE 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAVKrgPKTINLKAI--VDAALDQSSKDGVSVGICLTYDnSLATTREN--TKWQNGRDV-WWQD 335
Cdd:PRK06710 113 LEYQLHDSGAKVILCLDLVF--PRVTNVQSAtkIEHVIVTRIADFLPFPKNLLYP-FVQKKQSNlvVKVSESETIhLWNS 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 336 VISQYPTSCEVEwVDAEDPLFLL-YTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKS-TDVYWCTADCGWITGHSYVT 413
Cdd:PRK06710 190 VEKEVNTGVEVP-CDPENDLALLqYTGGTTGFPKGVMLTHKNLVSNTLMGVQWLYNCKEgEEVVLGVLPFFHVYGMTAVM 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLNGATVVVFegaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMrdDDKFVTRHSRKSLRVLGSVGEPINPSAWRW 493
Cdd:PRK06710 269 NLSIMQGYKMVLI---PKF-DMKMVFEAIKKHKVTLFPGAPTIYIALL--NSPLLKEYDISSIRACISGSAPLPVEVQEK 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FFNVVGDSrcpISDTWWQTETGGfmITPLPGAWPQK-PGSATFPFFGVQPVIVD-EKGNEIEGECSGYLCVKGSwpgafR 571
Cdd:PRK06710 343 FETVTGGK---LVEGYGLTESSP--VTHSNFLWEKRvPGSIGVPWPDTEAMIMSlETGEALPPGEIGEIVVKGP-----Q 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 572 TLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVK 651
Cdd:PRK06710 413 IMKGYWNKPEETAAVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYR 492
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 652 GQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEE 721
Cdd:PRK06710 493 GETVKAFVVLKEGTECSEE---ELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIEEEKRKNED 559
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
180-713 |
8.50e-29 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 120.87 E-value: 8.50e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWegnelgVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYL---PMLMELPIAMLACariGAVHSVVFAGF 256
Cdd:cd12118 18 PDRTSIVY------GDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLApntPAMYELHFGVPMA---GAVLNALNTRL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SADSLAQRIVDCKPNVILTcnavkrgpktinlkaivdaalDQSskdgvsvgicLTYDNSLATTRENTKWQngrdvWWQDv 336
Cdd:cd12118 89 DAEEIAFILRHSEAKVLFV---------------------DRE----------FEYEDLLAEGDPDFEWI-----PPAD- 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 isqyptscevEWvdaeDPLFLLYTSGSTGKPKGVLHT-TGGYMIYTATTfkYAFDYKSTDVYWCTAD----CGWItghsy 411
Cdd:cd12118 132 ----------EW----DPIALNYTSGTTGRPKGVVYHhRGAYLNALANI--LEWEMKQHPVYLWTLPmfhcNGWC----- 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 412 VTYGPMLNGATVVVFEGApnypDPGRCWDIVDKYKVSIFYTAPTlVRSLMRDDDKFVTRHSRKSLRVLgSVGEPINPS-- 489
Cdd:cd12118 191 FPWTVAAVGGTNVCLRKV----DAKAIYDLIEKHKVTHFCGAPT-VLNMLANAPPSDARPLPHRVHVM-TAGAPPPAAvl 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 490 --AWRWFFNVVgdsrcpisDTWWQTETGGFMITplpGAWpqKPGSATFP--------------FFGVQPVIVDEK----- 548
Cdd:cd12118 265 akMEELGFDVT--------HVYGLTETYGPATV---CAW--KPEWDELPteerarlkarqgvrYVGLEEVDVLDPetmkp 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 549 ----GNEIeGEC--SGYLCVKGSW--PGAFRTLFGDheryettyfkpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSG 620
Cdd:cd12118 332 vprdGKTI-GEIvfRGNIVMKGYLknPEATAEAFRG-------------GWFHSGDLAVIHPDGYIEIKDRSKDIIISGG 397
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 621 HRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPgLPK 700
Cdd:cd12118 398 ENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEE---EIIAFCREHLAGFMVPKTVVFGE-LPK 473
|
570
....*....|...
gi 378548265 701 TRSGKIMRRILRK 713
Cdd:cd12118 474 TSTGKIQKFVLRD 486
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
195-714 |
1.73e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 120.80 E-value: 1.73e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAqrivdckpnvil 274
Cdd:PRK07788 72 RGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQLA------------ 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 tcnavkrgpktinlkaivdaalDQSSKDGVSVgicLTYDNSLATTRENTKWQNGR-DVWWQDVISQYPTSCEVEWVD--- 350
Cdd:PRK07788 140 ----------------------EVAAREGVKA---LVYDDEFTDLLSALPPDLGRlRAWGGNPDDDEPSGSTDETLDdli 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 ---AEDPL--------FLLYTSGSTGKPKGVLH------TTGGyMIYTATTFKyafdykSTDVYWCTADCGWITGHSYVT 413
Cdd:PRK07788 195 agsSTAPLpkppkpggIVILTSGTTGTPKGAPRpepsplAPLA-GLLSRVPFR------AGETTLLPAPMFHATGWAHLT 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLnGATVVV---FegapnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSA 490
Cdd:PRK07788 268 LAMAL-GSTVVLrrrF-------DPEATLEDIAKHKATALVVVPVMLSRILDLGPEVLAKYDTSSLKIIFVSGSALSPEL 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 491 WRWFFNVVGDSRCpisDTWWQTETGgFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPgaf 570
Cdd:PRK07788 340 ATRALEAFGPVLY---NLYGSTEVA-FATIATPEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFP--- 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 rtlFgdhERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHE 649
Cdd:PRK07788 413 ---F---EGYTDGRDKQIIdGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDE 486
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 650 VKGQGIYAFVTLLEGVPYSEELRKSlvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKI 714
Cdd:PRK07788 487 EFGQRLRAFVVKAPGAALDEDAIKD---YVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREM 548
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
181-711 |
5.48e-28 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 118.14 E-value: 5.48e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHwegnelGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd12114 2 DATAVI------CGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAAR 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd12114 76 REAILADAGARLVLTDGPDAQLDVAVFDVLILDLDALAAPAPPPPVD--------------------------------- 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYM-IYTATTFKYA--------------FDYKSTDVYwctadcgw 405
Cdd:cd12114 123 --------VAPDDLAYVIFTSGSTGTPKGVMISHRAALnTILDINRRFAvgpddrvlalsslsFDLSVYDIF-------- 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsyvtyGPMLNGATVVVFEGAPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMrDDDKFVTRHSRkSLR-VLGSvGE 484
Cdd:cd12114 187 ---------GALSAGATLVLPDEARR-RDPAHWAELIERHGVTLWNSVPALLEMLL-DVLEAAQALLP-SLRlVLLS-GD 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 485 PIN---PSAWRWFF---NVV---GDSRCPISDTWWQtetggfmITPLPGAWPQKP-GsatFPFFGVQPVIVDEKGNEIEG 554
Cdd:cd12114 254 WIPldlPARLRALApdaRLIslgGATEASIWSIYHP-------IDEVPPDWRSIPyG---RPLANQRYRVLDPRGRDCPD 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 555 ECSGYLCVKGswPGAFRTLFGDHERYETTYFKPFAG--YYFSGD-GCSRDkDGYYWLTGRVDDVINVSGHRIGTAEVESA 631
Cdd:cd12114 324 WVPGELWIGG--RGVALGYLGDPELTAARFVTHPDGerLYRTGDlGRYRP-DGTLEFLGRRDGQVKVRGYRIELGEIEAA 400
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 632 LVLHPQCAEAAVVGIEhEVKGQGIYAFVTLLEGVP--YSEELRKSLVLMVRnqigAFAAPDRIHWAPGLPKTRSGKIMRR 709
Cdd:cd12114 401 LQAHPGVARAVVVVLG-DPGGKRLAAFVVPDNDGTpiAPDALRAFLAQTLP----AYMIPSRVIALEALPLTANGKVDRA 475
|
..
gi 378548265 710 IL 711
Cdd:cd12114 476 AL 477
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
181-712 |
7.44e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 118.06 E-value: 7.44e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELGvdasltYSELLQRVCQLANYLKDNGVKKGDAVVIYL---PMLMELpiaMLACARIGAVHSVVFAGFS 257
Cdd:PRK06145 17 DRAALVYRDQEIS------YAEFHQRILQAAGMLHARGIGQGDVVALLMknsAAFLEL---AFAASYLGAVFLPINYRLA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 258 ADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKdgvsvgicltydnslattrentkwqngrdvwwqdVI 337
Cdd:PRK06145 88 ADEVAYILGDAGAKLLLVDEEFDAIVALETPKIVIDAAAQADSR----------------------------------RL 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 338 SQYPTSCEVEWVDAEDPLF-LLYTSGSTGKPKGVLHTTGGymiytattfkyaFDYKSTDVywcTADCGWITGHSYVTYGP 416
Cdd:PRK06145 134 AQGGLEIPPQAAVAPTDLVrLMYTSGTTDRPKGVMHSYGN------------LHWKSIDH---VIALGLTASERLLVVGP 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 417 M-------LNGATVVVFEGA----PNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFvtRHSRKSLRVLGSVGEP 485
Cdd:PRK06145 199 LyhvgafdLPGIAVLWVGGTlrihREF-DPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRD--RFDLDSLAWCIGGGEK 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFFNVVGDSRcpISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGs 565
Cdd:PRK06145 276 TPESRIRDFTRVFTRAR--YIDAYGLTETCSGDTLMEAGREIEKIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRG- 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 566 wPGAFRTLFGDHERYETTYFkpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:PRK06145 353 -PKVTKGYWKDPEKTAEAFY---GDWFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIG 428
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 646 IEHEVKGQGIYAFVTLLEGVPYS-EELRKSlvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK06145 429 VHDDRWGERITAVVVLNPGATLTlEALDRH----CRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLR 492
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
351-712 |
9.96e-28 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 117.80 E-value: 9.96e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 AEDPL--FLLYTSGSTGKPKGV--------LHTTGGYMIYTATTFkyaFDYKSTDVYWCTADcgwITGHSYVTYGPMLN- 419
Cdd:PRK13390 145 TEQPCgaVMLYSSGTTGFPKGIqpdlpgrdVDAPGDPIVAIARAF---YDISESDIYYSSAP---IYHAAPLRWCSMVHa 218
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 -GATVVVfegAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVV 498
Cdd:PRK13390 219 lGGTVVL---AKRF-DAQATLGHVERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRAVIHAAAPCPVDVKHAMIDWL 294
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 GdsrcPISDTWWQ-TETGGFMITPLPgAWPQKPGSATFPFFGVQPvIVDEKGNEI-EGECSGYLCVKGSWPgaFRTLFGD 576
Cdd:PRK13390 295 G----PIVYEYYSsTEAHGMTFIDSP-DWLAHPGSVGRSVLGDLH-ICDDDGNELpAGRIGTVYFERDRLP--FRYLNDP 366
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 577 HERYETTY-FKPFagYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK13390 367 EKTAAAQHpAHPF--WTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQV 444
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 656 YAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK13390 445 KAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
627-705 |
4.38e-27 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 104.55 E-value: 4.38e-27
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 378548265 627 EVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVpysEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGK 705
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGV---ELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
181-713 |
9.15e-27 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 115.26 E-value: 9.15e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK07786 32 DAPALRFLGN------TTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPE 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILT-------CNAVKRGPKTINLKAIVDAAldqsSKDGVsvgicLTYDNSLAttrentkwQNGRDVWW 333
Cdd:PRK07786 106 IAFLVSDCGAHVVVTeaalapvATAVRDIVPLLSTVVVAGGS----SDDSV-----LGYEDLLA--------EAGPAHAP 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 334 QDVisqyptscevewvdAED-PLFLLYTSGSTGKPKG-VL-HTTggyMIYTATTFKYAFDY-KSTDVYWCTADCGWITGH 409
Cdd:PRK07786 169 VDI--------------PNDsPALIMYTSGTTGRPKGaVLtHAN---LTGQAMTCLRTNGAdINSDVGFVGVPLFHIAGI 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 410 SYVTYGPMLNGATVVVFEGAPnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfvTRHSRKSLRVLGSVGEPINPS 489
Cdd:PRK07786 232 GSMLPGLLLGAPTVIYPLGAF---DPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQ---ARPRDLALRVLSWGAAPASDT 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 490 AWRWFFNVVGDSRcpISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpga 569
Cdd:PRK07786 306 LLRQMAATFPEAQ--ILAAFGQTEMSPVTCMLLGEDAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAP---- 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 570 frTLFGDHERYETTYFKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEH 648
Cdd:PRK07786 380 --TLMSGYWNNPEATAEAFAGGWFhSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRAD 457
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 649 EVKGQGIYAFVTLLEGvpySEELR-KSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK07786 458 EKWGEVPVAVAAVRND---DAALTlEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRE 520
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
357-712 |
1.34e-26 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 114.01 E-value: 1.34e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDykstdvywctadCGWITGHSYVTYGPM-------------LNGATV 423
Cdd:cd05929 130 MLYSGGTTGRPKGIKRGLPGGPPDNDTLMAAALG------------FGPGADSVYLSPAPLyhaapfrwsmtalFMGGTL 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEgapNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPinpsawrwffnvvgdsrC 503
Cdd:cd05929 198 VLME---KF-DPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEAVRNAYDLSSLKRVIHAAAP-----------------C 256
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PIS-----DTWW---------QTETGGFmiTPLPGA-WPQKPGSATFPFFGVQPvIVDEKGNEIEGECSGYLCVKGSWPg 568
Cdd:cd05929 257 PPWvkeqwIDWGgpiiweyygGTEGQGL--TIINGEeWLTHPGSVGRAVLGKVH-ILDEDGNEVPPGEIGEVYFANGPG- 332
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 afrtlFGDHERYETTYFKPFAGYYFS-GDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:cd05929 333 -----FEYTNDPEKTAAARNEGGWSTlGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVP 407
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05929 408 DEELGQRVHAVVQPAPGADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
195-712 |
1.61e-26 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 114.03 E-value: 1.61e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVI- 273
Cdd:PRK12406 9 DRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLi 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 274 ----LTCNAVKRGPKTINLKAIVDAALDQSSkdgvsVGIcltyDNSLATTRENTkwqngrdVWWQDVISQYPTScevEWV 349
Cdd:PRK12406 89 ahadLLHGLASALPAGVTVLSVPTPPEIAAA-----YRI----SPALLTPPAGA-------IDWEGWLAQQEPY---DGP 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGgymiyTATTFKYAFDYKSTdVYwctadcGWITGHSYVTYGPMLNGA-------- 421
Cdd:PRK12406 150 PVPQPQSMIYTSGTTGHPKGVRRAAP-----TPEQAAAAEQMRAL-IY------GLKPGIRALLTGPLYHSApnayglra 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 422 ----TVVVFEgaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWR----W 493
Cdd:PRK12406 218 grlgGVLVLQ--PRF-DPEELLQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHAAAPCPADVKRamieW 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FFNVvgdsrcpISDTWWQTETGGfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAfrTL 573
Cdd:PRK12406 295 WGPV-------IYEYYGSTESGA-VTFATSEDALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRI--AGN--PD 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:PRK12406 363 FTYHNKPEKRAEIDRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGE 442
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 654 GIYAFVTLLEGVPYSEE-LRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK12406 443 ALMAVVEPQPGATLDEAdIRAQL----KARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
197-714 |
2.55e-26 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 114.17 E-value: 2.55e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPnvilt 275
Cdd:PLN02574 66 SISYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSV----- 140
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 cnavkrgpktinlkAIVDAALDQSSKDGvSVGIcltydnSLATTRENTKWQNGRDVW--WQDVISQYPTSCEVEWVDAED 353
Cdd:PLN02574 141 --------------GLAFTSPENVEKLS-PLGV------PVIGVPENYDFDSKRIEFpkFYELIKEDFDFVPKPVIKQDD 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLHTTGGY--MIYTATTFKYA-FDYKSTD-VYWCTADCGWITGHSYVTYGPMLNGATVVVFEGA 429
Cdd:PLN02574 200 VAAIMYSSGTTGASKGVVLTHRNLiaMVELFVRFEASqYEYPGSDnVYLAALPMFHIYGLSLFVVGLLSLGSTIVVMRRF 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 pnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVgdSRCPISDTW 509
Cdd:PLN02574 280 ----DASDMVKVIDRFKVTHFPVVPPILMALTKKA-KGVCGEVLKSLKQVSCGAAPLSGKFIQDFVQTL--PHVDFIQGY 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 510 WQTE-----TGGFMITPLpgawpQKPGSATFPFFGVQPVIVD-EKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETT 583
Cdd:PLN02574 353 GMTEstavgTRGFNTEKL-----SKYSSVGLLAPNMQAKVVDwSTGCLLPPGNCGELWIQG--PGVMKGYLNNPKATQST 425
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 584 YFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLE 663
Cdd:PLN02574 426 IDKD--GWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQ 503
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 378548265 664 GVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKI 714
Cdd:PLN02574 504 GSTLSQE---AVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKRS 551
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
181-718 |
9.07e-26 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 111.59 E-value: 9.07e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAG--FSA 258
Cdd:PRK03640 17 DRTAIEFE------EKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAV--AVLLNtrLSR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 DSLAQRIVDCKPNVILTcnavkrgpktinlkaivdaalDQSSKDGVSVGICLTYDNSLATTRENTKWQngrdvwwqdvis 338
Cdd:PRK03640 89 EELLWQLDDAEVKCLIT---------------------DDDFEAKLIPGISVKFAELMNGPKEEAEIQ------------ 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 339 qyptscevEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPML 418
Cdd:PRK03640 136 --------EEFDLDEVATIMYTSGTTGKPKGVIQTYGNHW-WSAVGSALNLGLTEDDCWLAAVPIFHISGLSILMRSVIY 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 nGATVVVFEGApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtRHSRKSLR--VLGsvGEPINPSawrwFFN 496
Cdd:PRK03640 207 -GMRVVLVEKF----DAEKINKLLQTGGVTIISVVSTMLQRLLERLGE---GTYPSSFRcmLLG--GGPAPKP----LLE 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 VVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEkGNEIEGECSGYLCVKGS--WPGAFRtlf 574
Cdd:PRK03640 273 QCKEKGIPVYQSYGMTETASQIVTLSPEDALTKLGSAGKPLFPCELKIEKD-GVVVPPFEEGEIVVKGPnvTKGYLN--- 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 gdheRYETTYfKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:PRK03640 349 ----REDATR-ETFQdGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQ 423
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 654 GIYAFVTLLEGVPySEELRkslvLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQ 718
Cdd:PRK03640 424 VPVAFVVKSGEVT-EEELR----HFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQLVEEM 483
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
181-711 |
1.22e-25 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 111.27 E-value: 1.22e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd17655 12 DHTAVVFE------DQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDYPEER 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAVKrgPKTINLKAIVDAALDQSSKDGVSvgicltydnSLAttrentkwqngrdvwwqdVISQy 340
Cdd:cd17655 86 IQYILEDSGADILLTQSHLQ--PPIAFIGLIDLLDEDTIYHEESE---------NLE------------------PVSK- 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvdAEDPLFLLYTSGSTGKPKGVLHTTGG-----------YMIYTATTF----KYAFDYKSTDVYwctadcgw 405
Cdd:cd17655 136 ----------SDDLAYVIYTSGSTGKPKGVMIEHRGvvnlvewankvIYQGEHLRValfaSISFDASVTEIF-------- 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsyvtyGPMLNGATVVVFEGAPNYPDPGRCwDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhSRKSLRVLGSVGEP 485
Cdd:cd17655 198 ---------ASLLSGNTLYIVRKETVLDGQALT-QYIRQNRITIIDLTPAHLKLLDAADDS-----EGLSLKHLIVGGEA 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFFNVVGDSrCPISDTWWQTETG-GFMITPLPGAWPQKP----GSatfPFFGVQPVIVDEKGNEIEGECSGYL 560
Cdd:cd17655 263 LSTELAKKIIELFGTN-PTITNAYGPTETTvDASIYQYEPETDQQVsvpiGK---PLGNTRIYILDQYGRPQPVGVAGEL 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 561 CVKGSwpGAFRtlfGDHERYETTYFK----PFAG---YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV 633
Cdd:cd17655 339 YIGGE--GVAR---GYLNRPELTAEKfvddPFVPgerMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLL 413
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 634 LHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPySEELRKSLvlmvRNQIGAFAAP------DRIhwapglPKTRSGKIM 707
Cdd:cd17655 414 QHPDIKEAVVIARKDEQGQNYLCAYIVSEKELP-VAQLREFL----ARELPDYMIPsyfiklDEI------PLTPNGKVD 482
|
....
gi 378548265 708 RRIL 711
Cdd:cd17655 483 RKAL 486
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
181-720 |
2.89e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 110.25 E-value: 2.89e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGvKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK07638 16 NKIAIKEN------DRVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDE 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcNAVKRGPKTINLKAIVDaaLDQsskdgvsvgicltydnslattrentkwqngrdvwWQDVISQY 340
Cdd:PRK07638 89 LKERLAISNADMIVT-ERYKLNDLPDEEGRVIE--IDE----------------------------------WKRMIEKY 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 -PTSCEVEwvDAE-DPLFLLYTSGSTGKPKGVLHTTGGYMiytattfkYAFDYKSTDVYWCTADCGWITG---HSYVTYG 415
Cdd:PRK07638 132 lPTYAPIE--NVQnAPFYMGFTSGSTGKPKAFLRAQQSWL--------HSFDCNVHDFHMKREDSVLIAGtlvHSLFLYG 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 416 P---MLNGATVVVFegaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDD------DKFVT------RHSRKSLRVlg 480
Cdd:PRK07638 202 AistLYVGQTVHLM---RKF-IPNQVLDKLETENISVMYTVPTMLESLYKENrvienkMKIISsgakweAEAKEKIKN-- 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 481 svgepINPSAWRWFFnvvgdsrcpisdtwWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYL 560
Cdd:PRK07638 276 -----IFPYAKLYEF--------------YGASELSFVTALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTV 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 561 CVKGswPGAFRTLFGDHERYETTyfkPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAE 640
Cdd:PRK07638 337 YVKS--PQFFMGYIIGGVLAREL---NADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDE 411
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 641 AAVVGIEHEVKGQGIYAFVtllEGVPYSEELRKslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLE 720
Cdd:PRK07638 412 IVVIGVPDSYWGEKPVAII---KGSATKQQLKS----FCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWIENQEK 484
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
349-713 |
6.01e-25 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 108.20 E-value: 6.01e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 349 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGpMLNGATVVVFEG 428
Cdd:cd05912 74 VKLDDIATIMYTSGTTGKPKGVQQTFGNHW-WSAIGSALNLGLTEDDNWLCALPLFHISGLSILMRS-VIYGMTVYLVDK 151
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 429 ApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLrvLGsvGEPINPSawrwFFNVVGDSRCPISDT 508
Cdd:cd05912 152 F----DAEQVLHLINSGKVTIISVVPTMLQRLLEILGEGYPNNLRCIL--LG--GGPAPKP----LLEQCKEKGIPVYQS 219
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 509 WWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GEcsgyLCVKGS--WPGAFRTLFGDHERYETTYF 585
Cdd:cd05912 220 YGMTETCSQIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQPPYEvGE----ILLKGPnvTKGYLNRPDATEESFENGWF 295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 586 KpfagyyfSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEgv 665
Cdd:cd05912 296 K-------TGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSER-- 366
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 378548265 666 PYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05912 367 PISEE---ELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
198-711 |
6.27e-25 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 108.71 E-value: 6.27e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcn 277
Cdd:cd17650 13 LTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYMLEDSGAKLLLT-- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDPLFL 357
Cdd:cd17650 91 ------------------------------------------------------------------------QPEDLAYV 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGYM-IYTATTFKYAFDYK----------STDVYwctadcgwiTGHSYVTygpMLNGATVVVf 426
Cdd:cd17650 99 IYTSGTTGKPKGVMVEHRNVAhAAHAWRREYELDSFpvrllqmasfSFDVF---------AGDFARS---LLNGGTLVI- 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 egAPN--YPDPGRCWDIVDKYKVSIFYTAPTLVRSLMrdddKFVTRHSRK--SLRVL--GSVGEPINPSAWRW------- 493
Cdd:cd17650 166 --CPDevKLDPAALYDLILKSRITLMESTPALIRPVM----AYVYRNGLDlsAMRLLivGSDGCKAQDFKTLAarfgqgm 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 -FFNVVGDSRCPISDTWWQTETGgfmitPLPGAWPQKPGSatfPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRT 572
Cdd:cd17650 240 rIINSYGVTEATIDSTYYEEGRD-----PLGDSANVPIGR---PLPNTAMYVLDERLQPQPVGVAGELYIGGA--GVARG 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 573 LFGD----HERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVgIEH 648
Cdd:cd17650 310 YLNRpeltAERFVENPFAPGERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVA-VRE 388
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 649 EVKGQ-GIYAFVTLLEgVPYSEELRKSLVlmvrNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17650 389 DKGGEaRLCAYVVAAA-TLNTAELRAFLA----KELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
192-721 |
6.46e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 109.45 E-value: 6.46e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 192 LGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPN 271
Cdd:PRK06164 30 IDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRGRAR 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 272 VIltcnAVKRGPKTINLKAIVDAALDQSSKD--GVSVgICLTYDNSLATTRentkwqnGRDVWWQDVISQYPTSCEVEWV 349
Cdd:PRK06164 110 WL----VVWPGFKGIDFAAILAAVPPDALPPlrAIAV-VDDAADATPAPAP-------GARVQLFALPDPAPPAAAGERA 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLY-TSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTAD-CGwITGHSYVTyGPMLNGATVV--- 424
Cdd:PRK06164 178 ADPDAGALLFtTSGTTSGPKLVLHRQAT-LLRHARAIARAYGYDPGAVLLAALPfCG-VFGFSTLL-GALAGGAPLVcep 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 425 VFEGApnypdpgRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtRHSRKSLRVLGSVGepiNPSAWRWFFNVVGDSRCP 504
Cdd:PRK06164 255 VFDAA-------RTARALRRHRVTHTFGNDEMLRRILDTAGE---RADFPSARLFGFAS---FAPALGELAALARARGVP 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 ISDTWWQTE-----TGGFMITP-----LPGAWPQKPgsatfpffGVQPVIVDEKGNEI--EGEcSGYLCVKGswPGAFRT 572
Cdd:PRK06164 322 LTGLYGSSEvqalvALQPATDPvsvriEGGGRPASP--------EARVRARDPQDGALlpDGE-SGEIEIRA--PSLMRG 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 573 LFGDHERYETTyFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEvkG 652
Cdd:PRK06164 391 YLDNPDATARA-LTD-DGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGATRD--G 466
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 653 QGI-YAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSG---KIMRRILRKIASRQLEE 721
Cdd:PRK06164 467 KTVpVAFVIPTDGASPDEA---GLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLREMAQARLAA 536
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
353-715 |
6.74e-25 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 106.26 E-value: 6.74e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHsYVTYGPMLNGATVVVFEGAP-- 430
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAAN-LLASAAGLHSRLGFGGGDSWLLSLPLYHVGGL-AILVRSLLAGAELVLLERNQal 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 431 --NYPDPGRCWdivdkykVSIfytAPTLVRSLMRDDdkfVTRHSRKSLRVLGSVGEPINPSAWRWFfnvvGDSRCPISDT 508
Cdd:cd17630 79 aeDLAPPGVTH-------VSL---VPTQLQRLLDSG---QGPAALKSLRAVLLGGAPIPPELLERA----ADRGIPLYTT 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 509 WWQTETGGfMITPLPGAWPQKPGSATfPFFGVQPVIVDEkgneiegecsGYLCVKGswpgafRTLFGDHERYETTYFKPF 588
Cdd:cd17630 142 YGMTETAS-QVATKRPDGFGRGGVGV-LLPGRELRIVED----------GEIWVGG------ASLAMGYLRGQLVPEFNE 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 589 AGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPyS 668
Cdd:cd17630 204 DGWFTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPAD-P 282
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 378548265 669 EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:cd17630 283 AELRAWL----KDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
353-708 |
1.19e-24 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 105.57 E-value: 1.19e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGYMiytattfkYAFDYKSTDVYWCTADCGWITG---HSYVTYGPM---LNGATVVVF 426
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERSWI--------ESFVCNEDLFNISGEDAILAPGplsHSLFLYGAIsalYLGGTFIGQ 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 EGApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRHSRKSLRvlgSVGEPINPSAWRWFFNVVGDSRcpIS 506
Cdd:cd17633 73 RKF----NPKSWIRKINQYNATVIYLVPTMLQALARTL---EPESKIKSIF---SSGQKLFESTKKKLKNIFPKAN--LI 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 507 DTWWQTETGgfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIegecsGYLCVKGswPGAFrtlfgdhERYETTYFK 586
Cdd:cd17633 141 EFYGTSELS--FITYNFNQESRPPNSVGRPFPNVEIEIRNADGGEI-----GKIFVKS--EMVF-------SGYVRGGFS 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 587 PFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQgIYAFVTLLEGVP 666
Cdd:cd17633 205 NPDGWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGE-IAVALYSGDKLT 283
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 378548265 667 YSEELRKSLVLMVRNQIgafaaPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17633 284 YKQLKRFLKQKLSRYEI-----PKKIIFVDSLPYTSSGKIAR 320
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
195-711 |
1.34e-24 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 107.34 E-value: 1.34e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd17652 10 DETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLADARPALLL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDP 354
Cdd:cd17652 90 T--------------------------------------------------------------------------TPDNL 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYK-----------STDVY---WCTAdcgwitghsyvtygpMLNG 420
Cdd:cd17652 96 AYVIYTSGSTGRPKGVVVTHRG-LANLAAAQIAAFDVGpgsrvlqfaspSFDASvweLLMA---------------LLAG 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEGAPNYPDPGRCwDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhsrkSLRVLGSVGEPINPS-AWRW-----F 494
Cdd:cd17652 160 ATLVLAPAEELLPGEPLA-DLLREHRITHVTLPPAALAALPPDDLP--------DLRTLVVAGEACPAElVDRWapgrrM 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 FNVVGDSRCPISDTWWQTETGGFMItPLPGAWPqkpgsatfpffGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRtlf 574
Cdd:cd17652 231 INAYGPTETTVCATMAGPLPGGGVP-PIGRPVP-----------GTRVYVLDARLRPVPPGVPGELYIAG--AGLAR--- 293
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 GDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGI 646
Cdd:cd17652 294 GYLNRPGLTAERfvadPFGApgsrMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVR 373
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 647 EHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17652 374 DDRPGDKRLVAYVVPAPGAaPTAAELRAHL----AERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
195-723 |
2.92e-24 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 109.66 E-value: 2.92e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK12316 2026 DQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLL 2105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCNAV-KRGPKTINLKaivdaaldqsskdgvsvgiCLTYDnslattrentkwqngRDVWWQDvisqYPTSCEVEWVDAED 353
Cdd:PRK12316 2106 TQRHLlERLPLPAGVA-------------------RLPLD---------------RDAEWAD----YPDTAPAVQLAGEN 2147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYafdYKSTDvywctADC--------------GWITghsyvtygPMLN 419
Cdd:PRK12316 2148 LAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGER---YELSP-----ADCelqfmsfsfdgaheQWFH--------PLLN 2211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVFEGApnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHsRKSLRVLGSVGEPINPSAWRWFFNVVG 499
Cdd:PRK12316 2212 GARVLIRDDE--LWDPEQLYDEMERHGVTILDFPPVYLQQLAEHAE--RDGR-PPAVRVYCFGGEAVPAASLRLAWEALR 2286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 DSRcpISDTWWQTETggfMITPLpgAW---PQKPGSATFPFFGvQPV------IVDEKGNEIEGECSGYLCVKGSwpGAF 570
Cdd:PRK12316 2287 PVY--LFNGYGPTEA---VVTPL--LWkcrPQDPCGAAYVPIG-RALgnrrayILDADLNLLAPGMAGELYLGGE--GLA 2356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 RtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:PRK12316 2357 R---GYLNRPGLTAERfvpdPFSAsgerLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAV 2433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 643 VVGIEhEVKGQGIYAFVTlleGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEEL 722
Cdd:PRK12316 2434 VVAQD-GASGKQLVAYVV---PDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQLRQA 2509
|
.
gi 378548265 723 G 723
Cdd:PRK12316 2510 Y 2510
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
199-724 |
3.19e-24 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 107.34 E-value: 3.19e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILT--- 275
Cdd:PRK08162 45 TWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFMLRHGEAKVLIVdte 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 -CNAVKRGPKTI-NLKAIVDAALDQSSKDGVSVGiCLTYDNSLATTRENTKWQNGRDVWwqDVISqyptscevewvdaed 353
Cdd:PRK08162 125 fAEVAREALALLpGPKPLVIDVDDPEYPGGRFIG-ALDYEAFLASGDPDFAWTLPADEW--DAIA--------------- 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 plfLLYTSGSTGKPKGVL-HTTGGYMIYTATTFkyAFDYKSTDVY-----------WCTAdcgW-ITghsyvtygpmLNG 420
Cdd:PRK08162 187 ---LNYTSGTTGNPKGVVyHHRGAYLNALSNIL--AWGMPKHPVYlwtlpmfhcngWCFP---WtVA----------ARA 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEGApnypDPGRCWDIVDKYKVSIFYTAPtLVRSLM---RDDDKFVTRHsrkslRVLGSVGEPINPSAwrwffnV 497
Cdd:PRK08162 249 GTNVCLRKV----DPKLIFDLIREHGVTHYCGAP-IVLSALinaPAEWRAGIDH-----PVHAMVAGAAPPAA------V 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 VGdsrcpisdtwwQTETGGFMITPLPG-----------AWpqKPGSATFPFF---------GV----------------Q 541
Cdd:PRK08162 313 IA-----------KMEEIGFDLTHVYGltetygpatvcAW--QPEWDALPLDeraqlkarqGVryplqegvtvldpdtmQ 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 542 PVIVDekgNEIEGEC--SGYLCVKGswpgafrtlfgdheryettYFK-------PFAGYYF-SGDGCSRDKDGYYWLTGR 611
Cdd:PRK08162 380 PVPAD---GETIGEImfRGNIVMKG-------------------YLKnpkateeAFAGGWFhTGDLAVLHPDGYIKIKDR 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 612 VDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDR 691
Cdd:PRK08162 438 SKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEE---EIIAHCREHLAGFKVPKA 514
|
570 580 590
....*....|....*....|....*....|...
gi 378548265 692 IHWAPgLPKTRSGKIMRRILRKIAsRQLEELGD 724
Cdd:PRK08162 515 VVFGE-LPKTSTGKIQKFVLREQA-KSLKAIDL 545
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
180-645 |
4.39e-24 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 107.49 E-value: 4.39e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEgnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:COG1022 25 PDRVALREK--EDGIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVPIYPTSSAE 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTCNA------VKRGPKTINLKAIVdaALDQssKDGVSVGICLTYDNSLATtrentkwqnGRDVWW 333
Cdd:COG1022 103 EVAYILNDSGAKVLFVEDQeqldklLEVRDELPSLRHIV--VLDP--RGLRDDPRLLSLDELLAL---------GREVAD 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 334 QDVISQYPTScevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVY-----WCtadcgWITG 408
Cdd:COG1022 170 PAELEARRAA-----VKPDDLATIIYTSGTTGRPKGVMLTHRN-LLSNARALLERLPLGPGDRTlsflpLA-----HVFE 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 409 HSyVTYGPMLNGATVVVFEGAPNYPD-------------PgRCW-----DIVDK------YKVSIFYTA----------- 453
Cdd:COG1022 239 RT-VSYYALAAGATVAFAESPDTLAEdlrevkptfmlavP-RVWekvyaGIQAKaeeaggLKRKLFRWAlavgrryarar 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 454 ------PTLVRSLMRDDDKFVTRHSRKSL----RVLGSVGEPINPSAWRwFFNVVGdsrCPISDTWWQTETGGFMITPLP 523
Cdd:COG1022 317 lagkspSLLLRLKHALADKLVFSKLREALggrlRFAVSGGAALGPELAR-FFRALG---IPVLEGYGLTETSPVITVNRP 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 524 GAwpQKPGSATFPFFGVQpVIVDEKGnEIegecsgylCVKGswPGAFRTLFGDHEryETTyfKPFA--GYYFSGD-GcSR 600
Cdd:COG1022 393 GD--NRIGTVGPPLPGVE-VKIAEDG-EI--------LVRG--PNVMKGYYKNPE--ATA--EAFDadGWLHTGDiG-EL 453
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 378548265 601 DKDGYYWLTGRVDDVINVS-GHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:COG1022 454 DEDGFLRITGRKKDLIVTSgGKNVAPQPIENALKASPLIEQAVVVG 499
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
197-720 |
6.44e-24 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 106.61 E-value: 6.44e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAGFSADSL-----AQRIvdcKPN 271
Cdd:PRK10946 48 QFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--PVNALFSHQRSelnayASQI---EPA 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 272 VILtcnaVKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLATTREntkwQNGRDVWWQdvisqyPTScevewvdA 351
Cdd:PRK10946 123 LLI----ADRQHALFSDDDFLNTLVAEHSSLRVVLLLNDDGEHSLDDAIN----HPAEDFTAT------PSP-------A 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHTTGGYmiytattfkyafDY---KSTDVYWCTAD----CGWITGHSYVTYGP-----MLN 419
Cdd:PRK10946 182 DEVAFFQLSGGSTGTPKLIPRTHNDY------------YYsvrRSVEICGFTPQtrylCALPAAHNYPMSSPgalgvFLA 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVfegAPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVG 499
Cdd:PRK10946 250 GGTVVL---APD-PSATLCFPLIEKHQVNVTALVPPAVSLWLQAIAEGGSRAQLASLKLLQVGGARLSETLARRIPAELG 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dsrCPIS----------------DTWWQT-ETGGFMITPLPGAWpqkpgsatfpffgvqpvIVDEKGNEI-EGEcSGYLC 561
Cdd:PRK10946 326 ---CQLQqvfgmaeglvnytrldDSDERIfTTQGRPMSPDDEVW-----------------VADADGNPLpQGE-VGRLM 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 562 VKGswPGAFRTLFGDHErYETTYFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEA 641
Cdd:PRK10946 385 TRG--PYTFRGYYKSPQ-HNASAFDA-NGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHA 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 642 AVVGIEHEVKGQGIYAFVTLLEGVPySEELRKSLvlmvRNQ-IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLE 720
Cdd:PRK10946 461 ALVSMEDELMGEKSCAFLVVKEPLK-AVQLRRFL----REQgIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRAS 535
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
195-743 |
7.58e-24 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 108.32 E-value: 7.58e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK12467 3118 DQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLL 3197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCNAV-KRGPKTINLKAIVdaaLDqsskdgvsvgicltydnslattrentkwqngRDVWWQdvisqYPTSCEVEWVDAED 353
Cdd:PRK12467 3198 TQAHLlEQLPAPAGDTALT---LD-------------------------------RLDLNG-----YSENNPSTRVMGEN 3238
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGV--------LHTTGGYMIY--TATT-----FKYAFDYKSTDVYWctadcgwitghsyvtygPML 418
Cdd:PRK12467 3239 LAYVIYTSGSTGKPKGVgvrhgalaNHLCWIAEAYelDANDrvllfMSFSFDGAQERFLW-----------------TLI 3301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 NGATVVVfegAPNYP-DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfvtRHSRKSLRVLGSVGEPINPSAW------ 491
Cdd:PRK12467 3302 CGGCLVV---RDNDLwDPEELWQAIHAHRISIACFPPAYLQQFAEDAG----GADCASLDIYVFGGEAVPPAAFeqvkrk 3374
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 492 ---RWFFNVVGDSRCPISDTWWQTETGGfmiTPLPGAWPqkpgsATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpG 568
Cdd:PRK12467 3375 lkpRGLTNGYGPTEAVVTVTLWKCGGDA---VCEAPYAP-----IGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGV--G 3444
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 AFRtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAE 640
Cdd:PRK12467 3445 LAR---GYHQRPSLTAERfvadPFSGsggrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVRE 3521
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 641 AAVVGIEHEvKGQGIYAFVTL-LEGVPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQL 719
Cdd:PRK12467 3522 AVVLARDGA-GGKQLVAYVVPaDPQGDWRETLRDHL----AASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDPDAKGS 3596
|
570 580
....*....|....*....|....*.
gi 378548265 720 EELgdtstLADPSVVDQLIAL--ADV 743
Cdd:PRK12467 3597 REY-----VAPRSEVEQQLAAiwADV 3617
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
195-713 |
1.06e-23 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 105.59 E-value: 1.06e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd05915 22 VHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 tcnavkrgpktinlkaiVDAALDQSSKDGVSVgicltydnsLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDP 354
Cdd:cd05915 102 -----------------FDPNLLPLVEAIRGE---------LKTVQHFVVMDEKAPEGYLAYEEALGEEADPVRVPERAA 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHT-TGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEgapnYP 433
Cdd:cd05915 156 CGMAYTTGTTGLPKGVVYShRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGP----RL 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 434 DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfVTRHSRKSLRVLGSVGEPinPSAWRWF-----FNVVGDSRCP---- 504
Cdd:cd05915 232 DPASLVELFDGEGVTFTAGVPTVWLALADYLES-TGHRLKTLRRLVVGGSAA--PRSLIARfermgVEVRQGYGLTetsp 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 --ISDTW---WQT--ETGGFMITPLPG-------AWPQKPGSATFPFFGVQPVIVDEKGNEIegeCSGYlcvkgswpgaf 570
Cdd:cd05915 309 vvVQNFVkshLESlsEEEKLTLKAKTGlpiplvrLRVADEEGRPVPKDGKALGEVQLKGPWI---TGGY----------- 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 rtlFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:cd05915 375 ---YGNEEATRSALTP--DGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPK 449
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 651 KGQGIYAFVTLLEGVPYSEElrksLVLMVRNQIGAFAA-PDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05915 450 WQERPLAVVVPRGEKPTPEE----LNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
171-721 |
1.91e-23 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 105.08 E-value: 1.91e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVeAGLGDKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV-- 248
Cdd:PRK05605 38 YDNAV-ARFGDRPALDFFG------ATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVvv 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 249 -HS------------------VVFAGFSADSLAQRIV-DCKPNVILTCNAVKRGP--KTINLKAIVDAALDQSSKDGVSV 306
Cdd:PRK05605 111 eHNplytahelehpfedhgarVAIVWDKVAPTVERLRrTTPLETIVSVNMIAAMPllQRLALRLPIPALRKARAALTGPA 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 307 GICLTYDNSLATTRentkwqnGRDvwwqDVISQYPTscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiytattfk 386
Cdd:PRK05605 191 PGTVPWETLVDAAI-------GGD----GSDVSHPR------PTPDDVALILYTSGTTGKPKGAQLTHRNLF-------- 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 387 yafdykstdvywCTADCG--WITG---------------HSY-----VTYGPMLnGATVVVFegaPNyPDPGRCWDIVDK 444
Cdd:PRK05605 246 ------------ANAAQGkaWVPGlgdgpervlaalpmfHAYgltlcLTLAVSI-GGELVLL---PA-PDIDLILDAMKK 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 445 YKVSIFYTAPTLVRSLMRdddkfVTRHSRKSLrvlgsvgepinpSAWRWFFNvvGDSRCPISDT-WWQTETGGFMI---- 519
Cdd:PRK05605 309 HPPTWLPGVPPLYEKIAE-----AAEERGVDL------------SGVRNAFS--GAMALPVSTVeLWEKLTGGLLVegyg 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 520 ----TPLPGAWP----QKPGSATFPFFGVQPVIVD-EKGNEI--EGEcSGYLCVKGswPGAFRtlfGDHERYETTYFKPF 588
Cdd:PRK05605 370 ltetSPIIVGNPmsddRRPGYVGVPFPDTEVRIVDpEDPDETmpDGE-EGELLVRG--PQVFK---GYWNRPEETAKSFL 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 589 AGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS 668
Cdd:PRK05605 444 DGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALD 523
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....
gi 378548265 669 EE-LRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEE 721
Cdd:PRK05605 524 PEgLRAYC----REHLTRYKVPRRFYHVDELPRDQLGKVRRREVREELLEKLGA 573
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
198-721 |
3.01e-23 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 106.58 E-value: 3.01e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILT-C 276
Cdd:PRK12316 4577 LTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTqS 4656
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 NAVKRGPktinlkaivdaaldqsSKDGVSvgiCLTYDnslattrentkwqngRDVWWQDvisqYPTSCEVEWVDAEDPLF 356
Cdd:PRK12316 4657 HLLQRLP----------------IPDGLA---SLALD---------------RDEDWEG----FPAHDPAVRLHPDNLAY 4698
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYafdykstdvYWCTADCGWITGHSYV-------TYGPMLNGATVVVfeGA 429
Cdd:PRK12316 4699 VIYTSGSTGRPKGVAVSHGSLVNHLHATGER---------YELTPDDRVLQFMSFSfdgshegLYHPLINGASVVI--RD 4767
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 PNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtRHSRKSLRVLGSVGEPINPS----AWR-----WFFNVVGD 500
Cdd:PRK12316 4768 DSLWDPERLYAEIHEHRVTVLVFPPVYLQQLAEHAER---DGEPPSLRVYCFGGEAVAQAsydlAWRalkpvYLFNGYGP 4844
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 501 SRCPISDTWWQTETGgfmitplpgawpQKPGSATFPFFGVQP----VIVDEKGNEIEGECSG--YLCVKGSWPGAFRTLF 574
Cdd:PRK12316 4845 TETTVTVLLWKARDG------------DACGAAYMPIGTPLGnrsgYVLDGQLNPLPVGVAGelYLGGEGVARGYLERPA 4912
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 GDHERYETTYF-KPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVkGQ 653
Cdd:PRK12316 4913 LTAERFVPDPFgAPGGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAV-GK 4991
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 654 GIYAFVT-----LLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEE 721
Cdd:PRK12316 4992 QLVGYVVpqdpaLADADEAQAELRDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKALPQPDASLLQQ 5064
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
197-715 |
8.44e-23 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 102.41 E-value: 8.44e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNgVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAGFSADSLAQR--IVDCKPNVIL 274
Cdd:cd05909 7 SLTYRKLLTGAIALARKLAKM-TKEGENVGVMLPPSAGGALANFALALSGKV--PVMLNYTAGLRELRacIKLAGIKTVL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCnavKRGPKTINLKAIVDAALDQ------------SSKDGVSVGICLTYdnslattrentkwqngRDVWWqdvISQYPT 342
Cdd:cd05909 84 TS---KQFIEKLKLHHLFDVEYDArivyledlrakiSKADKCKAFLAGKF----------------PPKWL---LRIFGV 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 343 SCevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYM--IYTATTFkyaFDYKSTDVYwctadCGWITG-HSY----VTYG 415
Cdd:cd05909 142 AP----VQPDDPAVILFTSGSEGLPKGVVLSHKNLLanVEQITAI---FDPNPEDVV-----FGALPFfHSFgltgCLWL 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 416 PMLNGATVVVfegAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRdddkFVTRHSRKSLRVLGSVGEPINPSAWRWFF 495
Cdd:cd05909 210 PLLSGIKVVF---HPNPLDYKKIPELIYDKKATILLGTPTFLRGYAR----AAHPEDFSSLRLVVAGAEKLKDTLRQEFQ 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 496 NVVGdsrCPISDTWWQTETGGFMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNE--IEGEcSGYLCVKGswPGAFRTL 573
Cdd:cd05909 283 EKFG---IRILEGYGTTECSPVISVNTPQS-PNKEGTVGRPLPGMEVKIVSVETHEevPIGE-GGLLLVRG--PNVMLGY 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FGDHERyetTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL-VLHPQCAEAAVVGIEHEVKG 652
Cdd:cd05909 356 LNEPEL---TSFAFGDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILsEILPEDNEVAVVSVPDGRKG 432
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 653 QGIYAFVTLLEGVPyseelrKSLVLMVRN-QIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:cd05909 433 EKIVLLTTTTDTDP------SSLNDILKNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTLKALA 490
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
352-705 |
9.22e-23 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 100.53 E-value: 9.22e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHTTG--GYMIYTATTFKYAFDYKSTDVYWCTAD---------CGWITGHSYVTYGPMLNG 420
Cdd:cd05924 3 ADDLYILYTGGTTGMPKGVMWRQEdiFRMLMGGADFGTGEFTPSEDAHKAAAAaagtvmfpaPPLMHGTGSWTAFGGLLG 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEGaPNYpDPGRCWDIVDKYKV-SIFYTAPTLVRSLMrddDKFVTRHSR--KSLRVLGSVGEPINPSAWRWFFNV 497
Cdd:cd05924 83 GQTVVLPD-DRF-DPEEVWRTIEKHKVtSMTIVGDAMARPLI---DALRDAGPYdlSSLFAISSGGALLSPEVKQGLLEL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 VGDSRcpISDTWWQTETGgFMITPLPGawPQKPGSATFPFFGVQPVIVDEKGNEIE--GECSGYLCVKGSWPGAFrtlFG 575
Cdd:cd05924 158 VPNIT--LVDAFGSSETG-FTGSGHSA--GSGPETGPFTRANPDTVVLDDDGRVVPpgSGGVGWIARRGHIPLGY---YG 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHERYETTyFKPFAG--YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:cd05924 230 DEAKTAET-FPEVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQ 308
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|..
gi 378548265 654 GIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGK 705
Cdd:cd05924 309 EVVAVVQLREGAGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
353-708 |
9.78e-23 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 100.04 E-value: 9.78e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVV--Fegap 430
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGN-LIAANLQLIHAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMekF---- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 431 nypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRwffnvvgdSRCPisDTWW 510
Cdd:cd17637 76 ---DPAEALELIEEEKVTLMGSFPPILSNLLDAAEK--SGVDLSSLRHVLGLDAPETIQRFE--------ETTG--ATFW 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 511 ----QTETGGFmITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETTYFK 586
Cdd:cd17637 141 slygQTETSGL-VTLSPYR--ERPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRG--PLVFQGYWNLPELTAYTFRN 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 587 pfaGYYFSGDGCSRDKDGYYWLTGRV--DDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEG 664
Cdd:cd17637 216 ---GWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPG 292
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 378548265 665 VPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17637 293 ATLTAD---ELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
199-713 |
1.53e-22 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 102.14 E-value: 1.53e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVviylpmlmelpiAMLA--CAR----------IGAVHSVVFAGFSADSLAQRIV 266
Cdd:PRK06018 41 TYAQIHDRALKVSQALDRDGIKLGDRV------------ATIAwnTWRhleawygimgIGAICHTVNPRLFPEQIAWIIN 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 267 DCKPNVILTcnavkrgpkTINLKAIVDAALDQSSKdgVSVGICLTYDNSL-ATTRENTkwqngrdVWWQDVISQYPTSCE 345
Cdd:PRK06018 109 HAEDRVVIT---------DLTFVPILEKIADKLPS--VERYVVLTDAAHMpQTTLKNA-------VAYEEWIAEADGDFA 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 346 VEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYT-ATTFKYAFDYKSTDVY-----------WCTADCGWITGHSYVT 413
Cdd:PRK06018 171 WKTFDENTAAGMCYTSGTTGDPKGVLYSHRSNVLHAlMANNGDALGTSAADTMlpvvplfhansWGIAFSAPSMGTKLVM 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLNGATVvvfegapnypdpgrcWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhSRKSLRVLGSVgepinpsawrw 493
Cdd:PRK06018 251 PGAKLDGASV---------------YELLDTEKVTFTAGVPTVWLMLLQYMEK-----EGLKLPHLKMV----------- 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 ffnVVGDSRCPIS-------------DTWWQTETGGF-MITPLPGAWPQKPGSAT--------FPFFGVQPVIVDEKGNE 551
Cdd:PRK06018 300 ---VCGGSAMPRSmikafedmgvevrHAWGMTEMSPLgTLAALKPPFSKLPGDARldvlqkqgYPPFGVEMKITDDAGKE 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 552 I--EGECSGYLCVKGswPGAFRTLFgdheRYETTYFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVE 629
Cdd:PRK06018 377 LpwDGKTFGRLKVRG--PAVAAAYY----RVDGEILDD-DGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLE 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 630 SALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:PRK06018 450 NLAVGHPKVAEAAVIGVYHPKWDERPLLIVQLKPGEtATREEILKYM----DGKIAKWWMPDDVAFVDAIPHTATGKILK 525
|
....*
gi 378548265 709 RILRK 713
Cdd:PRK06018 526 TALRE 530
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
198-711 |
1.81e-22 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 103.88 E-value: 1.81e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCN 277
Cdd:PRK12316 537 LDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLSQS 616
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AVKRgpktinlKAIVDAALDQsskdgvsvgicLTYDNSlattrenTKWQNGrdvwwqdvisqYPTSCEVEWVDAEDPLFL 357
Cdd:PRK12316 617 HLGR-------KLPLAAGVQV-----------LDLDRP-------AAWLEG-----------YSEENPGTELNPENLAYV 660
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGG---YMIYTATTFK------------YAFDYKSTDVYWctadcgwitghsyvtygPMLNGAT 422
Cdd:PRK12316 661 IYTSGSTGKPKGAGNRHRAlsnRLCWMQQAYGlgvgdtvlqktpFSFDVSVWEFFW-----------------PLMSGAR 723
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 423 VVVfEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrHSRKSLRVLGSVGEPINPSAW------RW--- 493
Cdd:PRK12316 724 LVV-AAPGDHRDPAKLVELINREGVDTLHFVPSMLQAFLQDEDV----ASCTSLRRIVCSGEALPADAQeqvfakLPqag 798
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FFNVVGDSRCPISDTWWQTETGGFMITPLpGAwpqkpgsatfPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRTL 573
Cdd:PRK12316 799 LYNLYGPTEAAIDVTHWTCVEEGGDSVPI-GR----------PIANLACYILDANLEPVPVGVLGELYLAGR--GLARGY 865
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FG----DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEhe 649
Cdd:PRK12316 866 HGrpglTAERFVPSPFVAGERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVD-- 943
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 650 vkGQGIYAFVtllegVPYSE--ELRKSLVLMVRNQIGAFAAPDriHWAP--GLPKTRSGKIMRRIL 711
Cdd:PRK12316 944 --GKQLVGYV-----VLESEggDWREALKAHLAASLPEYMVPA--QWLAleRLPLTPNGKLDRKAL 1000
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
527-712 |
2.06e-22 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 101.22 E-value: 2.06e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 527 PQKPGSATFPFFGVQPVIVDEKGNEI--EGECSGYLCVKGSwpgafrTLF-GDHERYETT--YFKPfAGYYFSGDGCSRD 601
Cdd:PRK07787 289 ERRPGWVGLPLAGVETRLVDEDGGPVphDGETVGELQVRGP------TLFdGYLNRPDATaaAFTA-DGWFRTGDVAVVD 361
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 602 KDGYYWLTGRVD-DVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEelrksLVLMVR 680
Cdd:PRK07787 362 PDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVAADE-----LIDFVA 436
|
170 180 190
....*....|....*....|....*....|..
gi 378548265 681 NQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK07787 437 QQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
197-645 |
3.74e-22 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 100.36 E-value: 3.74e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIltc 276
Cdd:cd05907 5 PITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKAL--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navkrgpktinlkaIVDaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDPLF 356
Cdd:cd05907 82 --------------FVE--------------------------------------------------------DPDDLAT 91
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDpg 436
Cdd:cd05907 92 IIYTSGTTGRPKGVMLSHRNIL-SNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYFASSAETLLD-- 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 437 rcwDIVdKYKVSIFYTAPTL---VRSLMRDDD------KFVTRHSRKSLRVLGSVGEPINPSAWRwFFNVVGdsrCPISD 507
Cdd:cd05907 169 ---DLS-EVRPTVFLAVPRVwekVYAAIKVKAvpglkrKLFDLAVGGRLRFAASGGAPLPAELLH-FFRALG---IPVYE 240
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 508 TWWQTETGGFMITPLPGAWpqKPGSATFPFFGVQPVIVDekgneiEGEcsgyLCVKGswPGAFRTLFGDHEryETTYFKP 587
Cdd:cd05907 241 GYGLTETSAVVTLNPPGDN--RIGTVGKPLPGVEVRIAD------DGE----ILVRG--PNVMLGYYKNPE--ATAEALD 304
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*....
gi 378548265 588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVI-NVSGHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:cd05907 305 ADGWLHTGDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLISQAVVIG 363
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
177-713 |
5.82e-22 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 100.39 E-value: 5.82e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 177 AGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGvKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFA-- 254
Cdd:cd05931 4 AARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPpt 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 255 -GFSADSLAQRIVDCKPNVILTCNAVKRGpktinlkaiVDAALDQSSKDGVSVGICLTydnSLATTRENTkwqngrdvwW 333
Cdd:cd05931 83 pGRHAERLAAILADAGPRVVLTTAAALAA---------VRAFAASRPAAGTPRLLVVD---LLPDTSAAD---------W 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 334 QDVIsqyptscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFK-YAFDYKSTDVYW--CTADCGWITGhs 410
Cdd:cd05931 142 PPPS-----------PDPDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRRaYGLDPGDVVVSWlpLYHDMGLIGG-- 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 yvTYGPMLNGATVVVFegAPNY--PDPGRcW-DIVDKYKVSIFYtAPT----LVRSLMRDDDKFVTRHSrkSLRVLGSVG 483
Cdd:cd05931 209 --LLTPLYSGGPSVLM--SPAAflRRPLR-WlRLISRYRATISA-APNfaydLCVRRVRDEDLEGLDLS--SWRVALNGA 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EPINPSAWRWFFN--------------------------VVGDSRCPISDTWWQTETGGfmitPLPGAWPQKPGSATF-- 535
Cdd:cd05931 281 EPVRPATLRRFAEafapfgfrpeafrpsyglaeatlfvsGGPPGTGPVVLRVDRDALAG----RAVAVAADDPAARELvs 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 536 ---PFFGVQPVIVDEKGNE-----IEGE--------CSGYLCVKGSWPGAFRTLFGDHERyettyfkpfaGYYFSGD-GC 598
Cdd:cd05931 357 cgrPLPDQEVRIVDPETGRelpdgEVGEiwvrgpsvASGYWGRPEATAETFGALAATDEG----------GWLRTGDlGF 426
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 599 SRDkdGYYWLTGRVDDVINVSGHRIGTAEVESAL-----VLHPQCAEAAVVGIEHEVKGqGIYAFVTLLEGVPYSEELRK 673
Cdd:cd05931 427 LHD--GELYITGRLKDLIIVRGRNHYPQDIEATAeeahpALRPGCVAAFSVPDDGEERL-VVVAEVERGADPADLAAIAA 503
|
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 378548265 674 SLVLMVRNQIGafAAPDRIHWAP--GLPKTRSGKIMRRILRK 713
Cdd:cd05931 504 AIRAAVAREHG--VAPADVVLVRpgSIPRTSSGKIQRRACRA 543
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
195-743 |
8.09e-22 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 101.96 E-value: 8.09e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK12316 3080 EQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLL 3159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TcnavkrgpktinlkaivDAALDQSSKDGVSVgICLTYDNSlattrentkwqngrdvwwqdvisQYPTSCEVEWVDAEDP 354
Cdd:PRK12316 3160 S-----------------QSHLRLPLAQGVQV-LDLDRGDE-----------------------NYAEANPAIRTMPENL 3198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYMIYT-ATTFKYAFDYKSTDVYWCTADcgwITGHSYVTYGPMLNGATVVVfEGAPNYP 433
Cdd:PRK12316 3199 AYVIYTSGSTGKPKGVGIRHSALSNHLcWMQQAYGLGVGDRVLQFTTFS---FDVFVEELFWPLMSGARVVL-AGPEDWR 3274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 434 DPGRCWDIVDKYKVSIfytaPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPS-AWRWF-----FNVVGDSRCPISD 507
Cdd:PRK12316 3275 DPALLVELINSEGVDV----LHAYPSMLQAFLEEEDAHRCTSLKRIVCGGEALPADlQQQVFaglplYNLYGPTEATITV 3350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 508 TWWQTETGGFMITPLPGAWPQKpgSATFPFFGVQPVIVDEKGNEiegecsgYLCVKGSWPGAFRTLFGDHERYETTYFKP 587
Cdd:PRK12316 3351 THWQCVEEGKDAVPIGRPIANR--ACYILDGSLEPVPVGALGEL-------YLGGEGLARGYHNRPGLTAERFVPDPFVP 3421
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVgiehEVKGQGIYAFVTLLEGVPy 667
Cdd:PRK12316 3422 GERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVL----AVDGRQLVAYVVPEDEAG- 3496
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 668 seELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEelgdTSTLADPSVVDQLIA--LADV 743
Cdd:PRK12316 3497 --DLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPDAALLQ----QDYVAPVNELERRLAaiWADV 3568
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
177-723 |
8.46e-22 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 99.85 E-value: 8.46e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 177 AGLGDKTAIHWEGNELGV----DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV---- 248
Cdd:PRK12583 21 GDAFDATVARFPDREALVvrhqALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAIlvni 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 249 ------------------HSVVFA-GFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVdaALDQSSKDGVsvgic 309
Cdd:PRK12583 101 npayraseleyalgqsgvRWVICAdAFKTSDYHAMLQELLPGLAEGQPGALACERLPELRGVV--SLAPAPPPGF----- 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 310 LTYDNSLATtrentkwqnGRDVWWQDVISQYPTscevewVDAEDPLFLLYTSGSTGKPKGVLHT-----TGGYMIYTATT 384
Cdd:PRK12583 174 LAWHELQAR---------GETVSREALAERQAS------LDRDDPINIQYTSGTTGFPKGATLShhnilNNGYFVAESLG 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 385 FKyafdykSTDV-------YWCTadcgwitGHSYVTYGPMLNGATVVVfegaPN-YPDPGRCWDIVDKYKVSIFYTAPTL 456
Cdd:PRK12583 239 LT------EHDRlcvpvplYHCF-------GMVLANLGCMTVGACLVY----PNeAFDPLATLQAVEEERCTALYGVPTM 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 457 VRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRwffNVVGDSRCP-ISDTWWQTETGGfmITPLPGAWPQKPGSATf 535
Cdd:PRK12583 302 FIAELDHPQR--GNFDLSSLRTGIMAGAPCPIEVMR---RVMDEMHMAeVQIAYGMTETSP--VSLQTTAADDLERRVE- 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 536 PFFGVQP----VIVDEKGNEI----EGE-CS-GYLCVKGSWPGAFRTLFGDHERyettyfkpfaGYYFSGDGCSRDKDGY 605
Cdd:PRK12583 374 TVGRTQPhlevKVVDPDGATVprgeIGElCTrGYSVMKGYWNNPEATAESIDED----------GWMHTGDLATMDEQGY 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 606 YWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKslvlMVRNQIG 684
Cdd:PRK12583 444 VRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASeEELRE----FCKARIA 519
|
570 580 590
....*....|....*....|....*....|....*....
gi 378548265 685 AFAAPDRIHWAPGLPKTRSGKIMRRILRKIAsrqLEELG 723
Cdd:PRK12583 520 HFKVPRYFRFVDEFPMTVTGKVQKFRMREIS---IEELA 555
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
198-739 |
1.43e-21 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 101.01 E-value: 1.43e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCN 277
Cdd:PRK12467 1600 LTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQS 1679
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AV-KRGPKTINLKAIVdaaLDQSskdgvsvgicltydnslattrentkwqngrdvwwQDVISQYPTSCEVEWVDAEDPLF 356
Cdd:PRK12467 1680 HLqARLPLPDGLRSLV---LDQE----------------------------------DDWLEGYSDSNPAVNLAPQNLAY 1722
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYM-IYTATTFKYAFdykstdvywCTADCgWITGHSYV-------TYGPMLNGATVVVfeG 428
Cdd:PRK12467 1723 VIYTSGSTGRPKGAGNRHGALVnRLCATQEAYQL---------SAADV-VLQFTSFAfdvsvweLFWPLINGARLVI--A 1790
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 429 APN-YPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVtrHSRkSLRVLGSVGEPINPSAWR-WF--------FNVV 498
Cdd:PRK12467 1791 PPGaHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVE--HPL-SLRRVVCGGEALEVEALRpWLerlpdtglFNLY 1867
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 GDSRCPISDTWWQTETGgfmitPLPGAWPQKPGSatfPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRtlfGDHE 578
Cdd:PRK12467 1868 GPTETAVDVTHWTCRRK-----DLEGRDSVPIGQ---PIANLSTYILDASLNPVPIGVAGELYLGGV--GLAR---GYLN 1934
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 579 RYETTYFK----PFAGY----YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:PRK12467 1935 RPALTAERfvadPFGTVgsrlYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQDGAN 2014
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 651 KGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAfAAPDRI---HWA--PGLPKTRSGKIMRRILRKIASRQLEelgdT 725
Cdd:PRK12467 2015 GKQLVAYVVPTDPGLVDDDEAQVALRAILKNHLKA-SLPEYMvpaHLVflARMPLTPNGKLDRKALPAPDASELQ----Q 2089
|
570
....*....|....
gi 378548265 726 STLADPSVVDQLIA 739
Cdd:PRK12467 2090 AYVAPQSELEQRLA 2103
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
196-713 |
2.04e-21 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 98.51 E-value: 2.04e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 196 ASLTYSE--LLQRvcQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVI 273
Cdd:PLN02246 49 RVYTYADveLLSR--RVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKASGAKLI 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 274 LTcnavkrgpktinLKAIVDAALDQSSKDGVSVgICL--TYDNSLATTrentkwqngrdvwwqDVISQYPTSCEVEWVDA 351
Cdd:PLN02246 127 IT------------QSCYVDKLKGLAEDDGVTV-VTIddPPEGCLHFS---------------ELTQADENELPEVEISP 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHTTGGYMiytaTTFKYAFDYKSTDVYWCTAD---CGWITGHSYVTYGPMLN----GATVV 424
Cdd:PLN02246 179 DDVVALPYSSGTTGLPKGVMLTHKGLV----TSVAQQVDGENPNLYFHSDDvilCVLPMFHIYSLNSVLLCglrvGAAIL 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 425 VFegaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPINPS---AWRwffnvvgdS 501
Cdd:PLN02246 255 IM---PKF-EIGALLELIQRHKVTIAPFVPPIVLAIAKSPV--VEKYDLSSIRMVLSGAAPLGKEledAFR--------A 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 502 RCP---ISDTWWQTETG-------GFMITPLPgawpQKPGSATFPFFGVQPVIVD-EKGNEIEGECSGYLCVKGswPGAF 570
Cdd:PLN02246 321 KLPnavLGQGYGMTEAGpvlamclAFAKEPFP----VKSGSCGTVVRNAELKIVDpETGASLPRNQPGEICIRG--PQIM 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 RTLFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:PLN02246 395 KGYLNDPEATANTIDK--DGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPSIADAAVVPMKDEV 472
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 651 KGQGIYAFVTLLEGVPYSEELRKSLvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PLN02246 473 AGEVPVAFVVRSNGSEITEDEIKQF---VAKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLRA 532
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
181-712 |
3.99e-21 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 97.64 E-value: 3.99e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIhwegnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK07514 17 DAPFI-----ETPDGLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAE 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILtCNAVKRGPktinLKAIVDAAldqsskdgvSVGICLTYD----NSLAttrentkwqngrdvwwqDV 336
Cdd:PRK07514 92 LDYFIGDAEPALVV-CDPANFAW----LSKIAAAA---------GAPHVETLDadgtGSLL-----------------EA 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWctadcgwitgHS------ 410
Cdd:PRK07514 141 AAAAPDDFETVPRGADDLAAILYTSGTTGRSKGAMLSHGN-LLSNALTLVDYWRFTPDDVLI----------HAlpifht 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 ---YV-TYGPMLNGATVVVFegaPNYpDPGRcwdIVDKY-KVSIFYTAPTLVRSLMRDD--DKFVTRHSRksLRVLGSVg 483
Cdd:PRK07514 210 hglFVaTNVALLAGASMIFL---PKF-DPDA---VLALMpRATVMMGVPTFYTRLLQEPrlTREAAAHMR--LFISGSA- 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 ePINPSAWRWFFNVVGDsrcPISDTWWQTETGgfMIT--PLPGAwpQKPGSATFPFFGVQPVIVD-EKGNEIEGECSGYL 560
Cdd:PRK07514 280 -PLLAETHREFQERTGH---AILERYGMTETN--MNTsnPYDGE--RRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMI 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 561 CVKGswPGAFRtlfGDHERYETTY--FKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQC 638
Cdd:PRK07514 352 EVKG--PNVFK---GYWRMPEKTAeeFRA-DGFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGV 425
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 639 AEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK07514 426 VESAVIGVPHPDFGEGVTAVVVPKPGAALDEA---AILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLR 496
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
193-713 |
5.25e-21 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 96.61 E-value: 5.25e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 193 GVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVfagfSADSLAQRIvdckpnv 272
Cdd:cd17653 18 SLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPL----DAKLPSARI------- 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 iltcnavkrgpktinlkaivDAALDQSSKDgvsvgICLTYDNslattrentkwqngrdvwwqdvisqyptscevewvdAE 352
Cdd:cd17653 87 --------------------QAILRTSGAT-----LLLTTDS------------------------------------PD 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGV-----------------LHTTGGYMIytATTFKYAFDYkstdvywCTAdcgwitghsyVTYG 415
Cdd:cd17653 106 DLAYIIFTSGSTGIPKGVmvphrgvlnyvsqpparLDVGPGSRV--AQVLSIAFDA-------CIG----------EIFS 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 416 PMLNGATVVVFEGAPNYPDPGRcwdivdkyKVSIFYTAPTLVRSLMRDDdkfvtrhsRKSLRVLGSVGEPINPS---AW- 491
Cdd:cd17653 167 TLCNGGTLVLADPSDPFAHVAR--------TVDALMSTPSILSTLSPQD--------FPNLKTIFLGGEAVPPSlldRWs 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 492 --RWFFNVVGDSRCPISDTWWQTETGGFMI--TPLPGAwpqkpgsatfpffGVqpVIVDEKGNEIEGECSGYLCVKGswP 567
Cdd:cd17653 231 pgRRLYNAYGPTECTISSTMTELLPGQPVTigKPIPNS-------------TC--YILDADLQPVPEGVVGEICISG--V 293
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 GAFRTLFGDHERyETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:cd17653 294 QVARGYLGNPAL-TASKFVPDPFWpgsrmYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTQA 372
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 643 VVgIEHEvkgQGIYAFVTllegvPYS---EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd17653 373 AA-IVVN---GRLVAFVT-----PETvdvDGLRSEL----AKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
352-712 |
1.11e-20 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 94.27 E-value: 1.11e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHT-----TGGYMIytattfKYAFDYKSTDV-------YWCTadcGWITGH-SYVTYGpml 418
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLThhnivNNGYFI------GERLGLTEQDRlcipvplFHCF---GSVLGVlACLTHG--- 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 ngATVVVfeGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLR--VLGsvGEPINPSAWRWFFN 496
Cdd:cd05917 70 --ATMVF--PSPSF-DPLAVLEAIEKEKCTALHGVPTMFIAELEHPDF--DKFDLSSLRtgIMA--GAPCPPELMKRVIE 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 VVGDSRCPISdtWWQTET--GGFMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNE-----IEGE--CSGYLCVKGSWp 567
Cdd:cd05917 141 VMNMKDVTIA--YGMTETspVSTQTRTDDSI-EKRVNTVGRIMPHTEAKIVDPEGGIvppvgVPGElcIRGYSVMKGYW- 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 gafrtlfGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:cd05917 217 -------NDPEKTAEAIDG--DGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVP 287
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEE-LRKslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05917 288 DERYGEEVCAWIRLKEGAELTEEdIKA----YCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
196-712 |
6.30e-20 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 94.35 E-value: 6.30e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 196 ASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAV--------------HSVVFAGFSA-- 258
Cdd:PRK08974 47 EVMTFRKLEERSRAFAAYLQNGlGLKKGDRVALMMPNLLQYPIALFGILRAGMIvvnvnplytpreleHQLNDSGAKAiv 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 -----DSLAQRIVDCKP--NVILT--CNAVKRGPKTInlkaiVDAALDQSSKdgvsvgICLTYDNSLATTRENTkWQNGR 329
Cdd:PRK08974 127 ivsnfAHTLEKVVFKTPvkHVILTrmGDQLSTAKGTL-----VNFVVKYIKR------LVPKYHLPDAISFRSA-LHKGR 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 330 DVwwqdvisQY--PTscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIytattfkyafdyksTDVYWCTAdcgwit 407
Cdd:PRK08974 195 RM-------QYvkPE------LVPEDLAFLQYTGGTTGVAKGAMLTHRN-ML--------------ANLEQAKA------ 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 408 ghsyvTYGPMLNGATVVVFEGAPNY---PDPGRCWDIVDKYKVSIFYTAPtlvrslmRDDDKFVTRHSRKSLRVLGSVGE 484
Cdd:PRK08974 241 -----AYGPLLHPGKELVVTALPLYhifALTVNCLLFIELGGQNLLITNP-------RDIPGFVKELKKYPFTAITGVNT 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 485 PINPSAWRWFFNVVGDSRCPIS-----------DTWWQTETG-----GFMIT---PLPGAWP----QKPGSATFPFFGVQ 541
Cdd:PRK08974 309 LFNALLNNEEFQELDFSSLKLSvgggmavqqavAERWVKLTGqylleGYGLTecsPLVSVNPydldYYSGSIGLPVPSTE 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 542 PVIVDEKGNEIEGECSGYLCVKGSwpgafRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGH 621
Cdd:PRK08974 389 IKLVDDDGNEVPPGEPGELWVKGP-----QVMLGYWQRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKKDMILVSGF 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 622 RIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSlvlmVRNQIGAFAAPDRIHWAPGLPKT 701
Cdd:PRK08974 464 NVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVVKKDPSLTEEELITH----CRRHLTGYKVPKLVEFRDELPKS 539
|
570
....*....|.
gi 378548265 702 RSGKIMRRILR 712
Cdd:PRK08974 540 NVGKILRRELR 550
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
198-728 |
6.31e-20 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 94.10 E-value: 6.31e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVI-------YLPMlmelpiaMLACARIGAVHSVVFAGFSADSLAQRIVDCKP 270
Cdd:PLN02860 33 RTGHEFVDGVLSLAAGLLRLGLRNGDVVAIaalnsdlYLEW-------LLAVACAGGIVAPLNYRWSFEEAKSAMLLVRP 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 271 NVILTCNAVKRGPKTINLKAI----VDAALDQSSKDGVSVgicltyDNSLATTRENTKWQNGrdvwwqdvisqyPTSCEV 346
Cdd:PLN02860 106 VMLVTDETCSSWYEELQNDRLpslmWQVFLESPSSSVFIF------LNSFLTTEMLKQRALG------------TTELDY 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 347 EWVdAEDPLFLLYTSGSTGKPKGVL--HTTggymIYTATTFKYAF-DYKSTDVYWCTADCGWITGHSYVTYGPMLNGATV 423
Cdd:PLN02860 168 AWA-PDDAVLICFTSGTTGRPKGVTisHSA----LIVQSLAKIAIvGYGEDDVYLHTAPLCHIGGLSSALAMLMVGACHV 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVfegaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVL----GSVGEPINPSAWRWFfnvvg 499
Cdd:PLN02860 243 LL----PKF-DAKAALQAIKQHNVTSMITVPAMMADLISLTRKSMTWKVFPSVRKIlnggGSLSSRLLPDAKKLF----- 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dSRCPISDTWWQTETGG---FMitPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRT--LF 574
Cdd:PLN02860 313 -PNAKLFSAYGMTEACSsltFM--TLHDPTLESPKQTLQTVNQTKSSSVHQPQGVCVGKPAPHVELKIGLDESSRVgrIL 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 --GDH--ERY-----ETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:PLN02860 390 trGPHvmLGYwgqnsETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVG 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 646 IEHEVKGQGIYAFVTLLEGVPYS----EELRKSLVL-------MVRNQ-IGAFAAPDRI-HWAPGLPKTRSGKIMRRILR 712
Cdd:PLN02860 470 VPDSRLTEMVVACVRLRDGWIWSdnekENAKKNLTLssetlrhHCREKnLSRFKIPKLFvQWRKPFPLTTTGKIRRDEVR 549
|
570
....*....|....*.
gi 378548265 713 KIASRQLEELgdTSTL 728
Cdd:PLN02860 550 REVLSHLQSL--PSNL 563
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
181-711 |
6.62e-20 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 95.62 E-value: 6.62e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK05691 1146 ERIALVWDG------GSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPDYPAER 1219
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAV-KRGPKTINLKAIvdaALDQsskdgvsvgicLTYDNslattrentkwqngrdvwwqdvisq 339
Cdd:PRK05691 1220 LAYMLADSGVELLLTQSHLlERLPQAEGVSAI---ALDS-----------LHLDS------------------------- 1260
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 YPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGG------YMIYTattfkYAFDykSTDVYWCTA---------DCG 404
Cdd:PRK05691 1261 WPSQAPGLHLHGDNLAYVIYTSGSTGQPKGVGNTHAAlaerlqWMQAT-----YALD--DSDVLMQKApisfdvsvwECF 1333
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 405 WitghsyvtygPMLNGATVVVfEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRhsrkSLRVLGSVGE 484
Cdd:PRK05691 1334 W----------PLITGCRLVL-AGPGEHRDPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAAACT----SLRRLFSGGE 1398
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 485 PInPSAWR----------WFFNVVGDSRCPISDTWWQTETGGFMITPLpgawpqkpGSatfPFFGVQPVIVDEKGNEIEG 554
Cdd:PRK05691 1399 AL-PAELRnrvlqrlpqvQLHNRYGPTETAINVTHWQCQAEDGERSPI--------GR---PLGNVLCRVLDAELNLLPP 1466
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 555 ECSGYLCVKGSwpGAFRTLFG----DHERY-ETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVE 629
Cdd:PRK05691 1467 GVAGELCIGGA--GLARGYLGrpalTAERFvPDPLGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQ 1544
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 630 SALVLHPQCAEAAVVgIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLVL-----MVRNQIGAFAApdrihwapgLPKTRS 703
Cdd:PRK05691 1545 ARLLAQPGVAQAAVL-VREGAAGAQLVGYYTGEAGQeAEAERLKAALAAelpeyMVPAQLIRLDQ---------MPLGPS 1614
|
....*...
gi 378548265 704 GKIMRRIL 711
Cdd:PRK05691 1615 GKLDRRAL 1622
|
|
| ACAS_N |
pfam16177 |
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many ... |
110-170 |
7.17e-20 |
|
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many acetyl-coenzyme A synthetase enzymes.
Pssm-ID: 465043 [Multi-domain] Cd Length: 55 Bit Score: 83.29 E-value: 7.17e-20
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 378548265 110 YMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVfsenLDVRKGPiSIEWFKGGITNICYNC 170
Cdd:pfam16177 1 YEALYRRSIEDPEGFWGEVAKELDWFKPF-DKV----LDGSNGP-FAKWFVGGKLNVCYNC 55
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
352-708 |
2.21e-19 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 90.01 E-value: 2.21e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGhsyvtygpMLNGATVVVFEGA-- 429
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGG--------LWWILTCLIHGGLcv 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 --PNYPDPGRCWDIVDKYKVSIFYTAPTL---VRSLMRDDDKFVtrhsrKSLRVLGSVGE-PINpsAWRWFFNVVGDSRc 503
Cdd:cd17635 73 tgGENTTYKSLFKILTTNAVTTTCLVPTLlskLVSELKSANATV-----PSLRLIGYGGSrAIA--ADVRFIEATGLTN- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 pISDTWWQTETGGFMITPLpGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWpgafrTLFGDHERYETT 583
Cdd:cd17635 145 -TAQVYGLSETGTALCLPT-DDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPA-----NMLGYWNNPERT 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 584 YFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLle 663
Cdd:cd17635 218 AEVLIDGWVNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVA-- 295
|
330 340 350 360
....*....|....*....|....*....|....*....|....*....
gi 378548265 664 gvpySEELRKS----LVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17635 296 ----SAELDENairaLKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
199-714 |
2.87e-19 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 91.82 E-value: 2.87e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILtcnA 278
Cdd:cd17642 46 SYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVF---C 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 279 VKRG-PKTINLkaivdaaldQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEwvdaEDPLFL 357
Cdd:cd17642 123 SKKGlQKVLNV---------QKKLKIIKTIIILDSKEDYKGYQCLYTFITQNLPPGFNEYDFKPPSFDRD----EQVALI 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHT--------------------TGGYMIYTATTFKYAFDYKSTDVYWCtadCGWitghsYVTYGPM 417
Cdd:cd17642 190 MNSSGSTGLPKGVQLThknivarfshardpifgnqiIPDTAILTVIPFHHGFGMFTTLGYLI---CGF-----RVVLMYK 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVvfegapnypdpgrcwDIVDKYKVSIFYTAPTLVRSLMRDddKFVTRHSRKSLRVLGSVGEPINPS---AWRWF 494
Cdd:cd17642 262 FEEELFL---------------RSLQDYKVQSALLVPTLFAFFAKS--TLVDKYDLSNLHEIASGGAPLSKEvgeAVAKR 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 FNVVGdsrcpISDTWWQTE-TGGFMITPlpgAWPQKPGSA--TFPFFGVQpVIVDEKGNEIEGECSGYLCVKGswPGAFR 571
Cdd:cd17642 325 FKLPG-----IRQGYGLTEtTSAILITP---EGDDKPGAVgkVVPFFYAK-VVDLDTGKTLGPNERGELCVKG--PMIMK 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 572 TLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVK 651
Cdd:cd17642 394 GYVNNPEATKALIDKD--GWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDA 471
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 652 GQGIYAFVTLLEGVPYSEelrKSLVLMVRNQIGAfAAPDR--IHWAPGLPKTRSGKIMRRILRKI 714
Cdd:cd17642 472 GELPAAVVVLEAGKTMTE---KEVMDYVASQVST-AKRLRggVKFVDEVPKGLTGKIDRRKIREI 532
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
183-711 |
5.78e-19 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 90.83 E-value: 5.78e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 183 TAIHWEGNELGVD--ASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK13383 44 TAARWPGRTAIIDddGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDA 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILtCNavkrgpktinlkaivDAALDQSSKDGVSVgicLTYDNSLATTREntkwqngrdvwwqdvisqy 340
Cdd:PRK13383 124 LAAALRAHHISTVV-AD---------------NEFAERIAGADDAV---AVIDPATAGAEE------------------- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptsCEVEWVDAEDPLFLLYTSGSTGKPKGVlhttggymiytattfKYAFDYKSTDVYWCT--ADCGWITGHSYVTYGPML 418
Cdd:PRK13383 166 ---SGGRPAVAAPGRIVLLTSGTTGKPKGV---------------PRAPQLRSAVGVWVTilDRTRLRTGSRISVAMPMF 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 NG-------------ATVVVFEgapnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEP 485
Cdd:PRK13383 228 HGlglgmlmltialgGTVLTHR----HFDAEAALAQASLHRADAFTAVPVVLARILELPPRVRARNPLPQLRVVMSSGDR 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFFNVVGDSrcpISDTWWQTETG-GFMITPLP-GAWPQKPGSatfPFFGVQPVIVDEKGNEIEGECSGYLCVK 563
Cdd:PRK13383 304 LDPTLGQRFMDTYGDI---LYNGYGSTEVGiGALATPADlRDAPETVGK---PVAGCPVRILDRNNRPVGPRVTGRIFVG 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 564 GSWPGafrtlfgdhERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:PRK13383 378 GELAG---------TRYTDGGGKAVVdGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNA 448
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 643 VVGIEHEVKGQGIYAFVTLLEGVPY-SEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK13383 449 VIGVPDERFGHRLAAFVVLHPGSGVdAAQLRDYL----KDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
195-711 |
8.93e-19 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 90.23 E-value: 8.93e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd17656 11 NQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVL 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCNAVKRGPKtinlkaivdaaldqsskdgvsvgicltyDNSLATTRENtkwqngrdvwwqDVISQYPTSCEVEWVDAEDP 354
Cdd:cd17656 91 TQRHLKSKLS----------------------------FNKSTILLED------------PSISQEDTSNIDYINNSDDL 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVL--HTTggymiyTATTFKYAFDYKSTDVYwctADCGWITGHSY-VTY----GPMLNGATVVVFE 427
Cdd:cd17656 131 LYIIYTSGTTGKPKGVQleHKN------MVNLLHFEREKTNINFS---DKVLQFATCSFdVCYqeifSTLLSGGTLYIIR 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 GAPNYpDPGRCWDIVDKYKVSIFYTaPTLVRSLMRDDDKFVTRHSrKSLRVLGSVGEPI---NPsawrwFFNVVGDSRCP 504
Cdd:cd17656 202 EETKR-DVEQLFDLVKRHNIEVVFL-PVAFLKFIFSEREFINRFP-TCVKHIITAGEQLvitNE-----FKEMLHEHNVH 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 ISDTWWQTET---GGFMITPLPgAWPQKPGSATfPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRTLFGD----H 577
Cdd:cd17656 274 LHNHYGPSEThvvTTYTINPEA-EIPELPPIGK-PISNTWIYILDQEQQLQPQGIVGELYISGA--SVARGYLNRqeltA 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 578 ERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYA 657
Cdd:cd17656 350 EKFFPDPFDPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCA 429
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 378548265 658 FVTLLEGVPySEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17656 430 YFVMEQELN-ISQLREYL----AKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
180-713 |
1.82e-18 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 89.55 E-value: 1.82e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:PRK08279 51 PDRPALLFE------DQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQRGA 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVIltcnavkrgpktinlkaIVDAALD---QSSKDGVSVGICLTYDNSLATTRENTkwqngrdvwWQDV 336
Cdd:PRK08279 125 VLAHSLNLVDAKHL-----------------IVGEELVeafEEARADLARPPRLWVAGGDTLDDPEG---------YEDL 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 ---ISQYPTSCEVEW--VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTAtTFKYAFDYKSTDVYWCT--------ADC 403
Cdd:PRK08279 179 aaaAAGAPTTNPASRsgVTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMG-GFGGLLRLTPDDVLYCClplyhntgGTV 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 404 GWitghsyvtyGPMLN-GATVVVFE--GAPNYpdpgrcWDIVDKYKVSIFYTAPTLVRSLM----RDDDKfvtRHsrkSL 476
Cdd:PRK08279 258 AW---------SSVLAaGATLALRRkfSASRF------WDDVRRYRATAFQYIGELCRYLLnqppKPTDR---DH---RL 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 477 RVLgsVGEPINPSAWRWFFNVVGDSRcpISDTWWQTEtGGFMITPLPGawpqKPGSATF-PFFGVQPV-IVD---EKGNE 551
Cdd:PRK08279 317 RLM--IGNGLRPDIWDEFQQRFGIPR--ILEFYAASE-GNVGFINVFN----FDGTVGRvPLWLAHPYaIVKydvDTGEP 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 552 IEGEcSGyLCVKGSwPGAFRTLFGdheryETTYFKPFAGY--------------------YF-SGDGCSRDKDGYYWLTG 610
Cdd:PRK08279 388 VRDA-DG-RCIKVK-PGEVGLLIG-----RITDRGPFDGYtdpeasekkilrdvfkkgdaWFnTGDLMRDDGFGHAQFVD 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 611 RVDDVI-----NVSghrigTAEVESALVLHPQCAEAAVVGIE-HEVKGQGIYAFVTLLEGVPYSeelRKSLVLMVRNQIG 684
Cdd:PRK08279 460 RLGDTFrwkgeNVA-----TTEVENALSGFPGVEEAVVYGVEvPGTDGRAGMAAIVLADGAEFD---LAALAAHLYERLP 531
|
570 580
....*....|....*....|....*....
gi 378548265 685 AFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK08279 532 AYAVPLFVRLVPELETTGTFKYRKVDLRK 560
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
181-717 |
1.25e-17 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 86.44 E-value: 1.25e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIH-WEGnelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAvhsvVFAGFSAD 259
Cdd:cd05918 14 DAPAVCaWDG-------SLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGG----AFVPLDPS 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIV----DCKPNVILTCnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqd 335
Cdd:cd05918 83 HPLQRLQeilqDTGAKVVLTS----------------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 336 visqyptscevewvDAEDPLFLLYTSGSTGKPKGVL--HTT--------GGYMIYTATT----F-KYAFDykstdvywct 400
Cdd:cd05918 104 --------------SPSDAAYVIFTSGSTGKPKGVVieHRAlstsalahGRALGLTSESrvlqFaSYTFD---------- 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 401 adcgwitGHSYVTYGPMLNGATVVVfegaPnyPDPGRCWDIVD---KYKVS-IFYTaPTLVRSLMRDDDkfvtrhsrKSL 476
Cdd:cd05918 160 -------VSILEIFTTLAAGGCLCI----P--SEEDRLNDLAGfinRLRVTwAFLT-PSVARLLDPEDV--------PSL 217
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 477 RVLGSVGEPINPSawrwffnVVgdsrcpisDTWWQ----------TETGGFMITPLPGAwPQKPGSATFPfFGVQPVIVD 546
Cdd:cd05918 218 RTLVLGGEALTQS-------DV--------DTWADrvrlinaygpAECTIAATVSPVVP-STDPRNIGRP-LGATCWVVD 280
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 547 EKGNE------------IEGE--CSGYL----CVKGSwpgafrtlFGDHERYETTYFKPFAGY-YFSGDGCSRDKDG--Y 605
Cdd:cd05918 281 PDNHDrlvpigavgellIEGPilARGYLndpeKTAAA--------FIEDPAWLKQEGSGRGRRlYRTGDLVRYNPDGslE 352
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 606 YwlTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGA 685
Cdd:cd05918 353 Y--VGRKDTQVKIRGQRVELGEIEHHLRQSLPGAKEVVVEVVKPKDGSSSPQLVAFVVLDGSSSGSGDGDSLFLEPSDEF 430
|
570 580 590 600
....*....|....*....|....*....|....*....|....*....
gi 378548265 686 FAAPDRI---------------HWAP--GLPKTRSGKIMRRILRKIASR 717
Cdd:cd05918 431 RALVAELrsklrqrlpsymvpsVFLPlsHLPLTASGKIDRRALRELAES 479
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
333-713 |
1.27e-17 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 86.66 E-value: 1.27e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 333 WQDVISQYP-TSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTG-----GYMIytATTFKYAFDykstDVYWCTAD---- 402
Cdd:PRK07867 132 WADELAAHRdAEPPFRVADPDDLFMLIFTSGTSGDPKAVRCTHRkvasaGVML--AQRFGLGPD----DVCYVSMPlfhs 205
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 403 ----CGWITGhsyvtygpMLNGATVVV---FEGAPNYPDpgrcwdiVDKYKVSIF-YTAPTLVRSLM---RDDDkfvtRH 471
Cdd:PRK07867 206 navmAGWAVA--------LAAGASIALrrkFSASGFLPD-------VRRYGATYAnYVGKPLSYVLAtpeRPDD----AD 266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 472 SrkSLRVL-GSVGEPINPSAWRWFFNvvgdsrCPISDTWWQTEtGGFMITPLPGAwpqKPGSATFPFFGVQ--------- 541
Cdd:PRK07867 267 N--PLRIVyGNEGAPGDIARFARRFG------CVVVDGFGSTE-GGVAITRTPDT---PPGALGPLPPGVAivdpdtgte 334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 542 --PVIVDEKGNEIEGECSGYLcVKGSWPGAFRTLFGDHEryeTTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVS 619
Cdd:PRK07867 335 cpPAEDADGRLLNADEAIGEL-VNTAGPGGFEGYYNDPE---ADAERMRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVD 410
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 620 GHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElRKSLVLMVRNQIGAFAAPDRIHWAPGLP 699
Cdd:PRK07867 411 GENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKFDPD-AFAEFLAAQPDLGPKQWPSYVRVCAELP 489
|
410
....*....|....
gi 378548265 700 KTRSGKIMRRILRK 713
Cdd:PRK07867 490 RTATFKVLKRQLSA 503
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
197-713 |
1.33e-17 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 86.96 E-value: 1.33e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsvvFAGFSADSLAQRIvdckpnviltc 276
Cdd:PLN02330 55 AVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGV----FSGANPTALESEI----------- 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLATTrentkwqngrdVWWQDVIS---QYPTSCEVEWVDAED 353
Cdd:PLN02330 120 ---KKQAEAAGAKLIVTNDTNYGKVKGLGLPVIVLGEEKIEGA-----------VNWKELLEaadRAGDTSDNEEILQTD 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLHT--------------TGGYMIYTATTFKYAFDYKstdVYWCTADCgwitghsyvtYGPMLN 419
Cdd:PLN02330 186 LCALPFSSGTTGISKGVMLThrnlvanlcsslfsVGPEMIGQVVTLGLIPFFH---IYGITGIC----------CATLRN 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVfegapnypdpgrcwdiVDKYKVSIFYTA------------PTLVRSLMRDD--DKFVTrhSRKSLRVLGSVGEP 485
Cdd:PLN02330 253 KGKVVV----------------MSRFELRTFLNAlitqevsfapivPPIILNLVKNPivEEFDL--SKLKLQAIMTAAAP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFfnvvgDSRCP---ISDTWWQTETGGFMIT---PLPGAWPQKPGSATFPFFGVQPVIVD-EKGNEIEGECSG 558
Cdd:PLN02330 315 LAPELLTAF-----EAKFPgvqVQEAYGLTEHSCITLThgdPEKGHGIAKKNSVGFILPNLEVKFIDpDTGRSLPKNTPG 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 559 YLCVKGSWpgAFRTLFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQC 638
Cdd:PLN02330 390 ELCVRSQC--VMQGYYNNKEETDRTIDE--DGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSV 465
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 639 AEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PLN02330 466 EDAAVVPLPDEEAGEIPAACVVINPKAKESEE---DILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKE 537
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
176-730 |
1.45e-17 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 87.01 E-value: 1.45e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 176 EAGLGDKTAIHwegnelgVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAG 255
Cdd:PRK06060 16 EAGWYDRPAFY-------AADVVTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPE 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 256 FSADSLAQRIVDCKPNVILTCNAVKRGPKTINlkaIVDAA--LDQSSKDGVSvgicltydnslattrentkwqngrdvww 333
Cdd:PRK06060 89 LHRDDHALAARNTEPALVVTSDALRDRFQPSR---VAEAAelMSEAARVAPG---------------------------- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 334 qdvisqyptscEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVT 413
Cdd:PRK06060 138 -----------GYEPMGGDALAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGNSV 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLNGATVVVfEGAPNYPDPGRCwdIVDKYKVSIFYTAPTLVRSLMrdddKFVTRHSRKSLRVLGSVGEPINPS-AWR 492
Cdd:PRK06060 207 WFPLATGGSAVI-NSAPVTPEAAAI--LSARFGPSVLYGVPNFFARVI----DSCSPDSFRSLRCVVSAGEALELGlAER 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 493 W--FFNVVgdsrcPISDTWWQTETGGFMITPLPGAWpqKPGS--ATFPFFGVQPVIVD--EKGNEIEGEcsgyLCVKG-- 564
Cdd:PRK06060 280 LmeFFGGI-----PILDGIGSTEVGQTFVSNRVDEW--RLGTlgRVLPPYEIRVVAPDgtTAGPGVEGD----LWVRGpa 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 565 ------SWPgafRTLFGDheryettyfkpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQC 638
Cdd:PRK06060 349 iakgywNRP---DSPVAN------------EGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAV 413
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 639 AEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR-KIASR 717
Cdd:PRK06060 414 AEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALRkQSPTK 493
|
570
....*....|...
gi 378548265 718 QLEELGDTSTLAD 730
Cdd:PRK06060 494 PIWELSLTEPGSG 506
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
357-708 |
3.28e-17 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 83.32 E-value: 3.28e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLhttggyMIYTATTFKYAfdykstdvYWCtaDCGWIT-GHSYVTYGP--------------MLNGA 421
Cdd:cd17638 5 IMFTSGTTGRSKGVM------CAHRQTLRAAA--------AWA--DCADLTeDDRYLIINPffhtfgykagivacLLTGA 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 422 TVV---VFegapnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMR--DDDKFvtrhSRKSLRvlgsvgepinpsawrwfFN 496
Cdd:cd17638 69 TVVpvaVF-------DVDAILEAIERERITVLPGPPTLFQSLLDhpGRKKF----DLSSLR-----------------AA 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 VVGDSRCPISDTWWQTETGGFMITpLPGAWPQKPGSATFPFFGVQPVIVDEK------GNEIEGECSGYLCVKGswPGAF 570
Cdd:cd17638 121 VTGAATVPVELVRRMRSELGFETV-LTAYGLTEAGVATMCRPGDDAETVATTcgracpGFEVRIADDGEVLVRG--YNVM 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 RTLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:cd17638 198 QGYLDDPEATAEAIDAD--GWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDER 275
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 651 KGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17638 276 MGEVGKAFVVARPGVTLTEE---DVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
198-713 |
1.15e-16 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 85.21 E-value: 1.15e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCN 277
Cdd:PRK12467 538 LSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQS 617
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AV-KRGPKTINLKAIVDAALDqsskdgvsvgicltydnslattrentkwqngrdvwwqDVISQYPTSCEVEWVDAEDPLF 356
Cdd:PRK12467 618 HLlAQLPVPAGLRSLCLDEPA-------------------------------------DLLCGYSGHNPEVALDPDNLAY 660
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYafdykstdvYWCTADCGWITGHSY-------VTYGPMLNGATVVVFeGA 429
Cdd:PRK12467 661 VIYTSGSTGQPKGVAISHGALANYVCVIAER---------LQLAADDSMLMVSTFafdlgvtELFGALASGATLHLL-PP 730
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 PNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRhSRKSLRVLGSVGEPINPSAWRWF------FNVVGDSRC 503
Cdd:PRK12467 731 DCARDAEAFAALMADQGVTVLKIVPSHLQALLQASRVALPR-PQRALVCGGEALQVDLLARVRALgpgarlINHYGPTET 809
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTETGGFMITPLPGAWPQkPGSATFpffgvqpvIVDEKGNEIEGECSGYLCVKGSwpGAFRtlfGDHERYETT 583
Cdd:PRK12467 810 TVGVSTYELSDEERDFGNVPIGQPL-ANLGLY--------ILDHYLNPVPVGVVGELYIGGA--GLAR---GYHRRPALT 875
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 584 --YF--KPFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK12467 876 aeRFvpDPFGAdggrLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPGDAGLQLV 955
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 656 YAFVTLL-----EGVPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK12467 956 AYLVPAAvadgaEHQATRDELKAQL----RQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPK 1014
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
590-723 |
1.39e-16 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 83.74 E-value: 1.39e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 590 GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSE 669
Cdd:PLN02479 430 GWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSD 509
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 670 E--LRKSLVLMVRNQIGAFAAPDRIHWAPgLPKTRSGKIMRRILRKIAsrqlEELG 723
Cdd:PLN02479 510 EaaLAEDIMKFCRERLPAYWVPKSVVFGP-LPKTATGKIQKHVLRAKA----KEMG 560
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
198-709 |
2.58e-16 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 82.63 E-value: 2.58e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL--T 275
Cdd:PRK05852 44 ISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPALPIAEQRVRSQAAGARVVLidA 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 CNAVKRGPKTINLKAIvdaALDQSSKDGVSVGICL-TYDNSLATTRENTKWQNGRDvwwqdvisqyptscevewvdaeDP 354
Cdd:PRK05852 124 DGPHDRAEPTTRWWPL---TVNVGGDSGPSGGTLSvHLDAATEPTPATSTPEGLRP----------------------DD 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYmiytattfkyafdykSTDVYWCTADCGWITGHSYVTYGPMLNGATVV-------VFE 427
Cdd:PRK05852 179 AMIMFTGGTTGLPKMVPWTHANI---------------ASSVRAIITGYRLSPRDATVAVMPLYHGHGLIaallatlASG 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 GAPNYPDPGRC-----WDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsr 502
Cdd:PRK05852 244 GAVLLPARGRFsahtfWDDIKAVGATWYTAVPTIHQILLERAATEPSGRKPAALRFIRSCSAPLTAETAQALQTEFA--- 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 503 CPISDTWWQTET---------GGFMITPLPGAWPQKPGSATfpffGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTL 573
Cdd:PRK05852 321 APVVCAFGMTEAthqvtttqiEGIGQTENPVVSTGLVGRST----GAQIRIVGSDGLPLPAGAVGEVWLRG--TTVVRGY 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FGDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:PRK05852 395 LGDPTITAANFTD---GWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGE 471
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 654 GIYAFVTLLEGVPYSeelRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRR 709
Cdd:PRK05852 472 AVAAVIVPRESAPPT---AEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
333-712 |
2.95e-16 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 82.38 E-value: 2.95e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 333 WQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGY-MIYTATTFKYafDYKSTDVYWCTADcgwiTGHS- 410
Cdd:PRK13388 131 YAELVAAAGALTPHREVDAMDPFMLIFTSGTTGAPKAVRCSHGRLaFAGRALTERF--GLTRDDVCYVSMP----LFHSn 204
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 --YVTYGPML-NGATVVV---FEGAPNYPDpgrcwdiVDKYKVSIF-YTAPTLVRSLM---RDDDkfvtrhSRKSLRV-L 479
Cdd:PRK13388 205 avMAGWAPAVaSGAAVALpakFSASGFLDD-------VRRYGATYFnYVGKPLAYILAtpeRPDD------ADNPLRVaF 271
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 GSVGEPINPSAWRWFFnvvgdsRCPISDTWWQTETGGfMITPLPGAwPqkPGSATFPFFGVqpVIVDEKGNEiegECSgy 559
Cdd:PRK13388 272 GNEASPRDIAEFSRRF------GCQVEDGYGSSEGAV-IVVREPGT-P--PGSIGRGAPGV--AIYNPETLT---ECA-- 334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 560 lcvkgswpgafRTLFGDH-----------ERYETTYFKPFAGYY---------------FSGDGCSRDKDGYYWLTGRVD 613
Cdd:PRK13388 335 -----------VARFDAHgallnadeaigELVNTAGAGFFEGYYnnpeataermrhgmyWSGDLAYRDADGWIYFAGRTA 403
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 614 DVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQ--IGAFAAPDR 691
Cdd:PRK13388 404 DWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDPD---AFAAFLAAQpdLGTKAWPRY 480
|
410 420
....*....|....*....|.
gi 378548265 692 IHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK13388 481 VRIAADLPSTATNKVLKRELI 501
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
193-645 |
3.55e-16 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 82.13 E-value: 3.55e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 193 GVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQrivdckpnv 272
Cdd:cd05932 2 GQVVEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRY--------- 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 ILTCNAVKrgpktinlkAIVDAALD--QSSKDGVSVGI--CLTYDNSLATTRENtkwqngrdvwWQDVISQYPTSCEVEW 348
Cdd:cd05932 73 VLEHSESK---------ALFVGKLDdwKAMAPGVPEGLisISLPPPSAANCQYQ----------WDDLIAQHPPLEERPT 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 349 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEG 428
Cdd:cd05932 134 RFPEQLATLIYTSGTTGQPKGVMLTFGSFA-WAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFAES 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 429 APNYPDPgrcwdiVDKYKVSIFYTAPTL-VRSLMRDDDK-----------------FVTRHSRKSL-----RVLGSVGEP 485
Cdd:cd05932 213 LDTFVED------VQRARPTLFFSVPRLwTKFQQGVQDKipqqklnlllkipvvnsLVKRKVLKGLgldqcRLAGCGSAP 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFFNVvgdsRCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEkgneiegecsGYLCVKGs 565
Cdd:cd05932 287 VPPALLEWYRSL----GLNILEAYGMTENFAYSHLNYPGR--DKIGTVGNAGPGVEVRISED----------GEILVRS- 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 566 wPGAFRTLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVS-GHRIGTAEVESALVLHPQCAEAAVV 644
Cdd:cd05932 350 -PALMMGYYKDPEATAEAFTAD--GFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVI 426
|
.
gi 378548265 645 G 645
Cdd:cd05932 427 G 427
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
195-712 |
3.68e-16 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 82.38 E-value: 3.68e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDC--KPNV 272
Cdd:PRK07059 46 GKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSgaEAIV 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 IL-----TCNAVKrgPKTiNLKAIVDAAL-DQSSKDGVSVGICLtydnslattRENTK----WQNGRDVWWQDVISQ--- 339
Cdd:PRK07059 126 VLenfatTVQQVL--AKT-AVKHVVVASMgDLLGFKGHIVNFVV---------RRVKKmvpaWSLPGHVRFNDALAEgar 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 ---YPTScevewVDAEDPLFLLYTSGSTGKPKG--VLHTT-GGYMIYTATTFKYAFDYKSTD-------------VYWCT 400
Cdd:PRK07059 194 qtfKPVK-----LGPDDVAFLQYTGGTTGVSKGatLLHRNiVANVLQMEAWLQPAFEKKPRPdqlnfvcalplyhIFALT 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 401 AdCGWITghsyvtygpMLNGATVVVFegaPNYPD-PGRCWDIvDKYKVSIFYTAPTLVRSLMRDDDkFVTRHSRKSLRVL 479
Cdd:PRK07059 269 V-CGLLG---------MRTGGRNILI---PNPRDiPGFIKEL-KKYQVHIFPAVNTLYNALLNNPD-FDKLDFSKLIVAN 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 G---SVGEPInpsAWRWFfNVVGdsrCPISDTWWQTETGGfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GE 555
Cdd:PRK07059 334 GggmAVQRPV---AERWL-EMTG---CPITEGYGLSETSP-VATCNPVDATEFSGTIGLPLPSTEVSIRDDDGNDLPlGE 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 556 cSGYLCVKGSwpgafRTLFGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVL 634
Cdd:PRK07059 406 -PGEICIRGP-----QVMAGYWNRPDETAKVMTAdGFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVAS 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 635 HPQCAEAAVVGIEHEVKGQGIYAFVtllegvpyseeLRKSLVLmVRNQIGAFAA--------PDRIHWAPGLPKTRSGKI 706
Cdd:PRK07059 480 HPGVLEVAAVGVPDEHSGEAVKLFV-----------VKKDPAL-TEEDVKAFCKerltnykrPKFVEFRTELPKTNVGKI 547
|
....*.
gi 378548265 707 MRRILR 712
Cdd:PRK07059 548 LRRELR 553
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
181-711 |
4.14e-16 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 81.71 E-value: 4.14e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHsvvfagfsads 260
Cdd:cd17644 15 DAVAVVFEDQQL------TYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAY----------- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 laqriVDCKPNViltcnavkrgPKTiNLKAIVDAAldqsskdGVSVgicltydnsLATTRENTkwqngrdvwwqdvisqy 340
Cdd:cd17644 78 -----VPLDPNY----------PQE-RLTYILEDA-------QISV---------LLTQPENL----------------- 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvdaedpLFLLYTSGSTGKPKGVL--------HTTGGYMIYTATT-------FKYAFDYKSTDVywctadcgw 405
Cdd:cd17644 109 --------------AYVIYTSGSTGKPKGVMiehqslvnLSHGLIKEYGITSsdrvlqfASIAFDVAAEEI--------- 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsYVTygpMLNGATVVVfegAPN--YPDPGRCWDIVDKYKVSIFYTAPTLVRSLMrDDDKFVTRHSRKSLRVLGSVG 483
Cdd:cd17644 166 -----YVT---LLSGATLVL---RPEemRSSLEDFVQYIQQWQLTVLSLPPAYWHLLV-LELLLSTIDLPSSLRLVIVGG 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EPINPSAWR-W---------FFNVVGDSRCPISDTWwqtetggFMITPLPGAWPQKPGSATfPFFGVQPVIVDEKGNEIE 553
Cdd:cd17644 234 EAVQPELVRqWqknvgnfiqLINVYGPTEATIAATV-------CRLTQLTERNITSVPIGR-PIANTQVYILDENLQPVP 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 554 GECSG--YLCVKGSWPGAFRTLFGDHERYETTYF--KPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVE 629
Cdd:cd17644 306 VGVPGelHIGGVGLARGYLNRPELTAEKFISHPFnsSESERLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIE 385
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 630 SALVLHPQCAEAAVVGIEHEVKGQGIYAF-VTLLEGVPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17644 386 AVLSQHNDVKTAVVIVREDQPGNKRLVAYiVPHYEESPSTVELRQFL----KAKLPDYMIPSAFVVLEELPLTPNGKIDR 461
|
...
gi 378548265 709 RIL 711
Cdd:cd17644 462 RAL 464
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
192-715 |
6.26e-16 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 81.41 E-value: 6.26e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 192 LGVdaSLTYSELLQRVCQLANYL-KDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV--------------HSVVFAGF 256
Cdd:PRK12492 46 LGV--TLSYAELERHSAAFAAYLqQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIvvntnplytaremrHQFKDSGA 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SA----DSLAQRIVDCKPN-VILTCNAVKRGPKTINLKA-IVDAALDQSSK--DGVSVGICLTYDNSLattrentkwQNG 328
Cdd:PRK12492 124 RAlvylNMFGKLVQEVLPDtGIEYLIEAKMGDLLPAAKGwLVNTVVDKVKKmvPAYHLPQAVPFKQAL---------RQG 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 329 RDvwwqdvISQYPTScevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTD------------- 395
Cdd:PRK12492 195 RG------LSLKPVP-----VGLDDIAVLQYTGGTTGLAKGAMLTHGN-LVANMLQVRACLSQLGPDgqplmkegqevmi 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 396 -------VYWCTADCGWItghsyvtygpMLNGATVVVFEgapNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFV 468
Cdd:PRK12492 263 aplplyhIYAFTANCMCM----------MVSGNHNVLIT---NPRDIPGFIKELGKWRFSALLGLNTLFVALMDHPGFKD 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 469 TRHSRksLRVLGSVGEP-INPSAWRWFfNVVGdsrCPISDTWWQTETggfmiTPLPGAWP----QKPGSATFPFFGVQPV 543
Cdd:PRK12492 330 LDFSA--LKLTNSGGTAlVKATAERWE-QLTG---CTIVEGYGLTET-----SPVASTNPygelARLGTVGIPVPGTALK 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 IVDEKGNEIE-GEcSGYLCVKGSwpgafRTLFGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGH 621
Cdd:PRK12492 399 VIDDDGNELPlGE-RGELCIKGP-----QVMKGYWQQPEATAEALDAeGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGF 472
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 622 RIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKslvlMVRNQIGAFAAPDRIHWAPGLPKT 701
Cdd:PRK12492 473 NVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDPGLSVEELKA----YCKENFTGYKVPKHIVLRDSLPMT 548
|
570
....*....|....
gi 378548265 702 RSGKIMRRILRKIA 715
Cdd:PRK12492 549 PVGKILRRELRDIA 562
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
198-715 |
8.36e-16 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 81.22 E-value: 8.36e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILtcn 277
Cdd:PLN03102 40 FTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILF--- 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 aVKRgpktiNLKAIVDAALDQSSKDGVSVGICLTYDNSLattrENTKWQNGRDVWWQDVISQ-YPTSCEVEWV----DAE 352
Cdd:PLN03102 117 -VDR-----SFEPLAREVLHLLSSEDSNLNLPVIFIHEI----DFPKRPSSEELDYECLIQRgEPTPSLVARMfriqDEH 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHT-TGGYMiyTATTFKYAFDYKSTDVYWCTA---DC-GWItghsyVTYGPMLNGATVVVFE 427
Cdd:PLN03102 187 DPISLNYTSGTTADPKGVVIShRGAYL--STLSAIIGWEMGTCPVYLWTLpmfHCnGWT-----FTWGTAARGGTSVCMR 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 --GAPnypdpgRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfvTRHSRKSLRVLGSVGEPINPSAW-----RWFFNVVgd 500
Cdd:PLN03102 260 hvTAP------EIYKNIEMHNVTHMCCVPTVFNILLKGNS---LDLSPRSGPVHVLTGGSPPPAALvkkvqRLGFQVM-- 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 501 srcpisDTWWQTE-TGGFMITPLPGAWPQKPGSATFPFFGVQPVI------VDEKGNEI------EGECSGYLCVKGSwp 567
Cdd:PLN03102 329 ------HAYGLTEaTGPVLFCEWQDEWNRLPENQQMELKARQGVSilgladVDVKNKETqesvprDGKTMGEIVIKGS-- 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 gafrTLFGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGI 646
Cdd:PLN03102 401 ----SIMKGYLKNPKATSEAFKhGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAM 476
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 647 EHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIG-------AFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:PLN03102 477 PHPTWGETPCAFVVLEKGETTKEDRVDKLVTRERDLIEycrenlpHFMCPRKVVFLQELPKNGNGKILKPKLRDIA 552
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
195-720 |
1.98e-15 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 80.86 E-value: 1.98e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK10252 481 RYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLI 560
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TcnavkrgpktinlkaivdAALDQSSKDGVSVGICLTYDNSLATtrentkwqngrdvwwQDVISQYPTSCevewvdaEDP 354
Cdd:PRK10252 561 T------------------TADQLPRFADVPDLTSLCYNAPLAP---------------QGAAPLQLSQP-------HHT 600
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVL--HT---------------TGGYMIYTATtfKYAFDYKSTDVYWctadcgwitghsyvtygPM 417
Cdd:PRK10252 601 AYIIFTSGSTGRPKGVMvgQTaivnrllwmqnhyplTADDVVLQKT--PCSFDVSVWEFFW-----------------PF 661
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVVfegAP--NYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWF- 494
Cdd:PRK10252 662 IAGAKLVM---AEpeAHRDPLAMQQFFAEYGVTTTHFVPSMLAAFVASLTPEGARQSCASLRQVFCSGEALPADLCREWq 738
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 -------FNVVGDSRCPISDTWWqtetggfmitPLPGAWPQKPGSAT----FPFFGVQPVIVDEKGNEIEGECSGYLCVK 563
Cdd:PRK10252 739 qltgaplHNLYGPTEAAVDVSWY----------PAFGEELAAVRGSSvpigYPVWNTGLRILDARMRPVPPGVAGDLYLT 808
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 564 GswpgafRTLF-GDHERYETTY-------FKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLH 635
Cdd:PRK10252 809 G------IQLAqGYLGRPDLTAsrfiadpFAPGERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQAL 882
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 636 PQCAEA---AVVGIEHEVKGQG---IYAFVTLLEGVPY-SEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:PRK10252 883 PDVEQAvthACVINQAAATGGDarqLVGYLVSQSGLPLdTSALQAQL----RERLPPHMVPVVLLQLDQLPLSANGKLDR 958
|
570
....*....|....*...
gi 378548265 709 RIL------RKIASRQLE 720
Cdd:PRK10252 959 KALplpelkAQVPGRAPK 976
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
191-706 |
2.16e-15 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 79.44 E-value: 2.16e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 191 ELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsvvFAGFSADSLAQRIVDCKP 270
Cdd:cd17654 10 QTTSDTTVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAA----YAPIDPASPEQRSLTVMK 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 271 NVILTCNAVKRgpKTINLKAIVDAALDQSSKdgvSVGICLTYdnslattrentkwqngrdvwwqdvisqyptscevewvd 350
Cdd:cd17654 86 KCHVSYLLQNK--ELDNAPLSFTPEHRHFNI---RTDECLAY-------------------------------------- 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 aedplfLLYTSGSTGKPKGVlHTTGGYMIYTATTFKYAFDYKSTDVYWCTadcgwitghSYVTYGP--------MLNGAT 422
Cdd:cd17654 123 ------VIHTSGTTGTPKIV-AVPHKCILPNIQHFRSLFNITSEDILFLT---------SPLTFDPsvveiflsLSSGAT 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 423 VVVFEGAPNyPDPGRCWDIVDK-YKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPInPS-----AWRWFFN 496
Cdd:cd17654 187 LLIVPTSVK-VLPSKLADILFKrHRITVLQATPTLFRRFGSQSIKSTVLSATSSLRVLALGGEPF-PSlvilsSWRGKGN 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 vvgdsRCPISDTWWQTETGGFMIT--------PLPGAwpqkpgsatFPFFGVQPVIVDEKGNEIEGEcsgyLCVKGSWPG 568
Cdd:cd17654 265 -----RTRIFNIYGITEVSCWALAykvpeedsPVQLG---------SPLLGTVIEVRDQNGSEGTGQ----VFLGGLNRV 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 AFRtlfgdhERYETTyfkPFAGYYFSGDGCSRdKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQcAEAAVVGIEh 648
Cdd:cd17654 327 CIL------DDEVTV---PKGTMRATGDFVTV-KDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLG-VESCAVTLS- 394
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 649 evKGQGIYAFVTlleGVPYSEELRKSLVLmvrNQIGAFAAPDRIHWAPGLPKTRSGKI 706
Cdd:cd17654 395 --DQQRLIAFIV---GESSSSRIHKELQL---TLLSSHAIPDTFVQIDKLPLTSHGKV 444
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
338-712 |
2.28e-15 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 79.75 E-value: 2.28e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 338 SQYPTSCEVEWVDAED-----PLF-------LLYTSGSTGKPKGVLHTTGGYMIYT-ATTFKYAFDYKSTDVY------- 397
Cdd:PRK07008 150 GSTPLLCYETLVGAQDgdydwPRFdenqassLCYTSGTTGNPKGALYSHRSTVLHAyGAALPDAMGLSARDAVlpvvpmf 229
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 398 ----WCTADCGWITGHSYVTYGPMLNGATVvvfegapnypdpgrcWDIVDKYKVSIFYTAPT----LVRSLMRDDDKFVT 469
Cdd:PRK07008 230 hvnaWGLPYSAPLTGAKLVLPGPDLDGKSL---------------YELIEAERVTFSAGVPTvwlgLLNHMREAGLRFST 294
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 470 rhsrksLR--VLGSVGEPinPSAWRWFFNVVGdsrCPISDTWWQTETGGF-MITPLPGAWPQKPGSATF--------PFF 538
Cdd:PRK07008 295 ------LRrtVIGGSACP--PAMIRTFEDEYG---VEVIHAWGMTEMSPLgTLCKLKWKHSQLPLDEQRkllekqgrVIY 363
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 539 GVQPVIVDEKGNEI--EGECSGYLCVKGSWpgafrtlfgdherYETTYFK----PF-AGYYFSGDGCSRDKDGYYWLTGR 611
Cdd:PRK07008 364 GVDMKIVGDDGRELpwDGKAFGDLQVRGPW-------------VIDRYFRgdasPLvDGWFPTGDVATIDADGFMQITDR 430
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 612 VDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVpysEELRKSLVLMVRNQIGAFAAPDR 691
Cdd:PRK07008 431 SKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGA---EVTREELLAFYEGKVAKWWIPDD 507
|
410 420
....*....|....*....|.
gi 378548265 692 IHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK07008 508 VVFVDAIPHTATGKLQKLKLR 528
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
181-711 |
2.30e-15 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 79.14 E-value: 2.30e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd17645 13 DHVAVVDRGQ------SLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGER 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd17645 87 IAYMLADSSAKILLT----------------------------------------------------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvDAEDPLFLLYTSGSTGKPKGVL--HTTggyMIYTATTFKYAFDYKSTDVYWCTADCGWiTGHSYVTYGPML 418
Cdd:cd17645 102 ---------NPDDLAYVIYTSGSTGLPKGVMieHHN---LVNLCEWHRPYFGVTPADKSLVYASFSF-DASAWEIFPHLT 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 NGATVVVFEGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhsrkSLRVLGSVGEPINPSAWRWF--FN 496
Cdd:cd17645 169 AGAALHVVPSERRL-DLDALNDYFNQEGITISFLPTGAAEQFMQLDNQ--------SLRVLLTGGDKLKKIERKGYklVN 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 VVGDSRCPIsdtwwqtetggfMITPLPGAWPQKPGSATFPFFGVQPVIVDEkGNEIEGE-CSGYLCVKGSwpGAFRTLFG 575
Cdd:cd17645 240 NYGPTENTV------------VATSFEIDKPYANIPIGKPIDNTRVYILDE-ALQLQPIgVAGELCIAGE--GLARGYLN 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 ----DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVK 651
Cdd:cd17645 305 rpelTAEKFIVHPFVPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADG 384
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 652 GQGIYAFVTLLEGVPYsEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17645 385 RKYLVAYVTAPEEIPH-EELREWL----KNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
195-713 |
3.70e-15 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 78.62 E-value: 3.70e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIvdckpnvil 274
Cdd:cd05939 1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCI--------- 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 tcnavkrgpKTINLKAIVDAALDqsskdgvsvgicltydnSLATTrentkwqngrdvwwqdvISQYPTSCEVewVDAEDP 354
Cdd:cd05939 72 ---------TVSKAKALIFNLLD-----------------PLLTQ-----------------SSTEPPSQDD--VNFRDK 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYMIYTATTFkYAFDYKSTDV-YWC-----TAdcGWITGHSYVtygpMLNGATVVVFE- 427
Cdd:cd05939 107 LFYIYTSGTTGLPKAAVIVHSRYYRIAAGAY-YAFGMRPEDVvYDClplyhSA--GGIMGVGQA----LLHGSTVVIRKk 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 -GAPNYpdpgrcWDIVDKYKVSIFYTAPTLVRSLM----RDDDKfvtRHsrkslRVLGSVGEPINPSAWRWF---FNV-- 497
Cdd:cd05939 180 fSASNF------WDDCVKYNCTIVQYIGEICRYLLaqppSEEEQ---KH-----NVRLAVGNGLRPQIWEQFvrrFGIpq 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 ----VGDSRCPISDTWWQTETG--GFMITPLPGAWPqkpgsatfpffgVQPVIVDEKGNEIEGECSGY--LCVKGSwPGA 569
Cdd:cd05939 246 igefYGATEGNSSLVNIDNHVGacGFNSRILPSVYP------------IRLIKVDEDTGELIRDSDGLciPCQPGE-PGL 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 570 F----------RTLFG------DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV 633
Cdd:cd05939 313 LvgkiiqndplRRFDGyvnegaTNKKIARDVFKKGDSAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILS 392
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 634 LHPQCAEAAVVGIE-HEVKGQ-GIYAFVTLLEGVP---YSEELRKSLVlmvrnqigAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd05939 393 NVLGLEDVVVYGVEvPGVEGRaGMAAIVDPERKVDldrFSAVLAKSLP--------PYARPQFIRLLPEVDKTGTFKLQK 464
|
....*
gi 378548265 709 RILRK 713
Cdd:cd05939 465 TDLQK 469
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
197-715 |
4.46e-15 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 78.77 E-value: 4.46e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYL-KDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILT 275
Cdd:PRK08751 50 TITYREADQLVEQFAAYLlGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVV 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 ----CNAVKRGPKTINLKAIVDAALDQ--SSKDGVSVGICLTYDNSLATtrentKWQNGRDVWWQDVISQ-YPTSCEVEW 348
Cdd:PRK08751 130 idnfGTTVQQVIADTPVKQVITTGLGDmlGFPKAALVNFVVKYVKKLVP-----EYRINGAIRFREALALgRKHSMPTLQ 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 349 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKyafdykstdvyWCTADCGWITGHSYV-TYGPML-------NG 420
Cdd:PRK08751 205 IEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQ-----------WLAGTGKLEEGCEVViTALPLYhifaltaNG 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEGA----PNYPD-PGRCWDIvDKYKVSIFYTAPTLVRSLMR----DDDKFvtrhsrKSLRVLGSVGEPINPS-A 490
Cdd:PRK08751 274 LVFMKIGGCnhliSNPRDmPGFVKEL-KKTRFTAFTGVNTLFNGLLNtpgfDQIDF------SSLKMTLGGGMAVQRSvA 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 491 WRWFfNVVGdsrCPISDTWWQTETG-GFMITPLpgAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GECsGYLCVKGSwpg 568
Cdd:PRK08751 347 ERWK-QVTG---LTLVEAYGLTETSpAACINPL--TLKEYNGSIGLPIPSTDACIKDDAGTVLAiGEI-GELCIKGP--- 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 afRTLFGDHER-YETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:PRK08751 417 --QVMKGYWKRpEETAKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVP 494
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEELRKSlvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:PRK08751 495 DEKSGEIVKVVIVKKDPALTAEDVKAH----ARANLTGYKQPRIIEFRKELPKTNVGKILRRELRDAA 558
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
194-719 |
5.87e-15 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 78.27 E-value: 5.87e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 194 VDASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAG--FSA--------DSLA 262
Cdd:PRK05677 46 LGKTLTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLI--VVNTNplYTAremehqfnDSGA 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 263 QRIVdCKPNviLTCNAVKRGPKTINLKAIVDAALDQSSK-DGVSVGICLTYDNSLATTrentkWQNGRDVWWQDVISQ-- 339
Cdd:PRK05677 124 KALV-CLAN--MAHLAEKVLPKTGVKHVIVTEVADMLPPlKRLLINAVVKHVKKMVPA-----YHLPQAVKFNDALAKga 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 YPTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHT-------------------TGGYMIYTATTFKYafdykstDVYWCT 400
Cdd:PRK05677 196 GQPVTEAN-PQADDVAVLQYTGGTTGVAKGAMLThrnlvanmlqcralmgsnlNEGCEILIAPLPLY-------HIYAFT 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 401 ADCGWItghsyvtygpMLNGATVVVFegaPNYPD-PGRCWDIvDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRksLRVL 479
Cdd:PRK05677 268 FHCMAM----------MLIGNHNILI---SNPRDlPAMVKEL-GKWKFSGFVGLNTLFVALCNNEAFRKLDFSA--LKLT 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 GSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETggfmiTPLPGAWPQK---PGSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:PRK05677 332 LSGGMALQLATAERWKEVTG---CAICEGYGMTET-----SPVVSVNPSQaiqVGTIGIPVPSTLCKVIDDDGNELPLGE 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGswPGAFRtlfGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLH 635
Cdd:PRK05677 404 VGELCVKG--PQVMK---GYWQRPEATDEILDSdGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAAL 478
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 636 PQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:PRK05677 479 PGVLQCAAIGVPDEKSGEAIKVFVVVKPGETLTKE---QVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELRDEE 555
|
....
gi 378548265 716 SRQL 719
Cdd:PRK05677 556 LKKA 559
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
180-713 |
6.25e-15 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 78.29 E-value: 6.25e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGNELgvdASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSA 258
Cdd:PRK05620 24 GDTTVTTWGGAEQ---EQTTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMN 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 DSL-------AQRIVDCKP-------NVILTCNAVKR----GPKTINLKAIVDAaldqsskDGVSvgiCLTYDNSLattr 320
Cdd:PRK05620 101 DQIvhiinhaEDEVIVADPrlaeqlgEILKECPCVRAvvfiGPSDADSAAAHMP-------EGIK---VYSYEALL---- 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 321 entkwqNGRdvwwqdvisqyptSCEVEW--VDAEDPLFLLYTSGSTGKPKGV-------------LHTTGGYMIYTATTF 385
Cdd:PRK05620 167 ------DGR-------------STVYDWpeLDETTAAAICYSTGTTGAPKGVvyshrslylqslsLRTTDSLAVTHGESF 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 386 kyafdYKSTDVY----WCTADCGWITGHSYVTYGPMLNGATVVvfegapnypdpgrcwDIVDKYKVSIFYTAPTLVRSLM 461
Cdd:PRK05620 228 -----LCCVPIYhvlsWGVPLAAFMSGTPLVFPGPDLSAPTLA---------------KIIATAMPRVAHGVPTLWIQLM 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 462 RDDDKfvTRHSRKSLRVLGSVGEPINP---SAWRWFFNVvgdsrcPISDTWWQTETG--GFMITPLPGA-----WPQKPG 531
Cdd:PRK05620 288 VHYLK--NPPERMSLQEIYVGGSAVPPiliKAWEERYGV------DVVHVWGMTETSpvGTVARPPSGVsgearWAYRVS 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 532 SATFPFfGVQPVIVDEkGNEIEG--ECSGYLCVKGSW-------------PGAFRTLFGDHERYETTYFKPfAGYYFSGD 596
Cdd:PRK05620 360 QGRFPA-SLEYRIVND-GQVMEStdRNEGEIQVRGNWvtasyyhspteegGGAASTFRGEDVEDANDRFTA-DGWLRTGD 436
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 597 GCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLV 676
Cdd:PRK05620 437 VGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETAERLR 516
|
570 580 590
....*....|....*....|....*....|....*..
gi 378548265 677 LMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK05620 517 DQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLRQ 553
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
353-704 |
3.41e-14 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 74.26 E-value: 3.41e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGYMIYTattfkyafdykstdvyWCTADCGWITGHS-YVTYGPM-----LNGATVVVF 426
Cdd:cd17636 1 DPVLAIYTAAFSGRPNGALLSHQALLAQA----------------LVLAVLQAIDEGTvFLNSGPLfhigtLMFTLATFH 64
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 EGAPNY----PDPGRCWDIVDKYKVS-IFYTAPTL--VRSLMRDDdkfvtRHSRKSLRVLgsvgepinPSAWRWFFNVvg 499
Cdd:cd17636 65 AGGTNVfvrrVDAEEVLELIEAERCThAFLLPPTIdqIVELNADG-----LYDLSSLRSS--------PAAPEWNDMA-- 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dsrcPISDTWW--------QTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEI-EGEcSGYLCVKGswPGAF 570
Cdd:cd17636 130 ----TVDTSPWgrkpggygQTEVMGLATFAALGG--GAIGGAGRPSPLVQVRILDEDGREVpDGE-VGEIVARG--PTVM 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 RTLFGdheRYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:cd17636 201 AGYWN---RPEVNARRTRGGWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPR 277
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 378548265 651 KGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSG 704
Cdd:cd17636 278 WAQSVKAIVVLKPGASVTEA---ELIEHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
199-713 |
6.89e-14 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 74.78 E-value: 6.89e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLaqriVDCkpnvILTCN 277
Cdd:cd05937 7 TYSETYDLVLRYAHWLHDDlGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPL----IHC----LKLSG 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AvkrgpktinlKAIVdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewVDAEDPLFL 357
Cdd:cd05937 79 S----------RFVI--------------------------------------------------------VDPDDPAIL 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGgyMIY-TATTFKYAFDYKSTD-VYWC----------TADCGWITGHSYVTYGP--------- 416
Cdd:cd05937 93 IYTSGTTGLPKAAAISWR--RTLvTSNLLSHDLNLKNGDrTYTCmplyhgtaafLGACNCLMSGGTLALSRkfsasqfwk 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 417 --MLNGATVVVFEG--------APNYPDP-----------GRCWDIVDKYK----VSI---FYTAPTLVRSLmrdddkfv 468
Cdd:cd05937 171 dvRDSGATIIQYVGelcryllsTPPSPYDrdhkvrvawgnGLRPDIWERFRerfnVPEigeFYAATEGVFAL-------- 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 469 TRHSRKSLrVLGSVGEpiNPSAWRWFFN-----VVGDsrcPISDTWWQTETGGFMI-TPLpgawpQKPGSATFPFFGVQp 542
Cdd:cd05937 243 TNHNVGDF-GAGAIGH--HGLIRRWKFEnqvvlVKMD---PETDDPIRDPKTGFCVrAPV-----GEPGEMLGRVPFKN- 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 543 vivdekgneiegecsgylcvKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHR 622
Cdd:cd05937 311 --------------------REAFQGYLHNEDATESKLVRDVFRKGDIYFRTGDLLRQDADGRWYFLDRLGDTFRWKSEN 370
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 623 IGTAEVESALVLHPQCAEAAVVGIE---HEvkGQGIYAFVTLLEGVPYSEELRKS-LVLMVRNQIGAFAAPDRIHWAPGL 698
Cdd:cd05937 371 VSTTEVADVLGAHPDIAEANVYGVKvpgHD--GRAGCAAITLEESSAVPTEFTKSlLASLARKNLPSYAVPLFLRLTEEV 448
|
570
....*....|....*
gi 378548265 699 PKTRSGKIMRRILRK 713
Cdd:cd05937 449 ATTDNHKQQKGVLRD 463
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
177-714 |
7.39e-14 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 75.01 E-value: 7.39e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 177 AGLGDKTAIHWEGNELGVDAsLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACarigavhsvVFAGF 256
Cdd:cd05906 20 AERGPTKGITYIDADGSEEF-QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWAC---------VLAGF 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SAdslaqrivdckpnVILTCNAVKRGPKTINLKAI-VDAALDQsskdgvsvGICLTYDNSLATTRENTKWQnGRDVWWQD 335
Cdd:cd05906 90 VP-------------APLTVPPTYDEPNARLRKLRhIWQLLGS--------PVVLTDAELVAEFAGLETLS-GLPGIRVL 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 336 VISQYP-TSCEVEWV--DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKyAFDYKSTDVY--WCTADcgWITGHS 410
Cdd:cd05906 148 SIEELLdTAADHDLPqsRPDDLALLMLTSGSTGFPKAVPLTHRNILARSAGKIQ-HNGLTPQDVFlnWVPLD--HVGGLV 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 YVTYGPMLNG------ATVVVFEgapnypDPGRCWDIVDKYKVSIFYtAPTLVRSLMRDD-DKFVTRH-SRKSLRVLGSV 482
Cdd:cd05906 225 ELHLRAVYLGcqqvhvPTEEILA------DPLRWLDLIDRYRVTITW-APNFAFALLNDLlEEIEDGTwDLSSLRYLVNA 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 483 GEPINPSAWRWFFNVVGDSRCP---ISDTWWQTETGGFMITPLPGAWPQKPGSATF-----PFFGVQPVIVDEKGNEIEG 554
Cdd:cd05906 298 GEAVVAKTIRRLLRLLEPYGLPpdaIRPAFGMTETCSGVIYSRSFPTYDHSQALEFvslgrPIPGVSMRIVDDEGQLLPE 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 555 ECSGYLCVKGswPGAFRTLFGDHERYETTYFKpfAGYYFSGD-GCSRDkdGYYWLTGRVDDVINVSGHRIGTAEVESAL- 632
Cdd:cd05906 378 GEVGRLQVRG--PVVTKGYYNNPEANAEAFTE--DGWFRTGDlGFLDN--GNLTITGRTKDTIIVNGVNYYSHEIEAAVe 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 633 ---VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGafAAPDR-IHWAPG-LPKTRSGKIM 707
Cdd:cd05906 452 evpGVEPSFTAAFAVRDPGAETEELAIFFVPEYDLQDALSETLRAIRSVVSREVG--VSPAYlIPLPKEeIPKTSLGKIQ 529
|
....*..
gi 378548265 708 RRILRKI 714
Cdd:cd05906 530 RSKLKAA 536
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
196-711 |
1.47e-13 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 73.89 E-value: 1.47e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 196 ASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILT 275
Cdd:PRK05857 40 SALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPIAAIERFCQITDPAAALV 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 CNAVKRGPKTI--NLKAIVDAALDQSSKDGVSVgicltydNSLATTRENTKWQNGrdvwwqdvisqyptscevewvdAED 353
Cdd:PRK05857 120 APGSKMASSAVpeALHSIPVIAVDIAAVTRESE-------HSLDAASLAGNADQG----------------------SED 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLhttggymIYTATTFKYAFDYKSTDVYWCTadcgWITGHSyvTYGPM---------------L 418
Cdd:PRK05857 171 PLAMIFTSGTTGEPKAVL-------LANRTFFAVPDILQKEGLNWVT----WVVGET--TYSPLpathigglwwiltclM 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 NGATVVVfeGAPNypdPGRCWDIVDKYKVSIFYTAPTLVRSLMrDDDKFvTRHSRKSLRVLGSVGEPINPSAWRwFFNVV 498
Cdd:PRK05857 238 HGGLCVT--GGEN---TTSLLEILTTNAVATTCLVPTLLSKLV-SELKS-ANATVPSLRLVGYGGSRAIAADVR-FIEAT 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 GdsrCPISDTWWQTETGGFMITpLP---GAWPQ-KPGSATFPFFGVQPVIVDEKG---NEIEGECS---GYLCVKGswPG 568
Cdd:PRK05857 310 G---VRTAQVYGLSETGCTALC-LPtddGSIVKiEAGAVGRPYPGVDVYLAATDGigpTAPGAGPSasfGTLWIKS--PA 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 AFRTLFGDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEH 648
Cdd:PRK05857 384 NMLGYWNNPERTAEVLID---GWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPD 460
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 649 EVKGqgiyAFVTLLEgVPYSE-------ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK05857 461 EEFG----ALVGLAV-VASAEldesaarALKHTIAARFRRESEPMARPSTIVIVTDIPRTQSGKVMRASL 525
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
585-713 |
1.11e-12 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 70.07 E-value: 1.11e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 585 FKPFA--GYYFSGDGCSRDkDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVtll 662
Cdd:PRK07824 227 PDPFAepGWFRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAV--- 302
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|.
gi 378548265 663 EGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK07824 303 VGDGGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
195-647 |
1.26e-12 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 70.46 E-value: 1.26e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIl 274
Cdd:cd05940 1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHL- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 tcnavkrgpktinlkaIVDAAldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaedp 354
Cdd:cd05940 80 ----------------VVDAA----------------------------------------------------------- 84
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 lFLLYTSGSTGKPKGVLHTTGGYmIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFE--GAPNY 432
Cdd:cd05940 85 -LYIYTSGTTGLPKAAIISHRRA-WRGGAFFAGSGGALPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRKkfSASNF 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 433 pdpgrcWDIVDKYKVSIFYTAPTLVRSLM----RDDDKfvtRHsrkSLRVLgsVGEPINPSAWRWFFNVVGDSRcpISDT 508
Cdd:cd05940 163 ------WDDIRKYQATIFQYIGELCRYLLnqppKPTER---KH---KVRMI--FGNGLRPDIWEEFKERFGVPR--IAEF 226
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 509 WWQTE--TGGFMITPLPGAWPQKPgSATFPFFGVQPVIVD-EKGNEIEGEcSGYL--CVKGSwPGAFRTLFGDHERYEtT 583
Cdd:cd05940 227 YAATEgnSGFINFFGKPGAIGRNP-SLLRKVAPLALVKYDlESGEPIRDA-EGRCikVPRGE-PGLLISRINPLEPFD-G 302
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 584 YFKPFA--------------GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:cd05940 303 YTDPAAtekkilrdvfkkgdAWFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQ 380
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
356-743 |
2.52e-12 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 70.97 E-value: 2.52e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 356 FLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYaFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVfegaPN--YP 433
Cdd:PRK05691 3873 YVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPY-LALSEADVIAQTASQSFDISVWQFLAAPLFGARVEIV----PNaiAH 3947
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 434 DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfvtRHSRKSLRVLGSVGEPINPS-AWRWF--------FNVVGDSRCP 504
Cdd:PRK05691 3948 DPQGLLAHVQAQGITVLESVPSLIQGMLAED-----RQALDGLRWMLPTGEAMPPElARQWLqrypqiglVNAYGPAECS 4022
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 ISDTWWQTETGGFMITPLPGAWPQKPGsatfpffgvQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRTLFGDHERYETTY 584
Cdd:PRK05691 4023 DDVAFFRVDLASTRGSYLPIGSPTDNN---------RLYLLDEALELVPLGAVGELCVAGT--GVGRGYVGDPLRTALAF 4091
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 585 F-KPFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAvVGIEHEVKGQGIYAFV 659
Cdd:PRK05691 4092 VpHPFGApgerLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAA-VAVQEGVNGKHLVGYL 4170
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 660 TLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEElgdTSTLADPSVVDQLIA 739
Cdd:PRK05691 4171 VPHQTVLAQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKALPALDIGQLQS---QAYLAPRNELEQTLA 4247
|
....*.
gi 378548265 740 --LADV 743
Cdd:PRK05691 4248 tiWADV 4253
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
544-725 |
9.49e-12 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 68.30 E-value: 9.49e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 IVDEKGNEI-----EGE--CSGYLCVKGSWPGAFRTlfgdHERYETtyfkpfAGYYFSGDGCSRDKDGYYWLTGRVDDVI 616
Cdd:PRK08315 384 IVDPETGETvprgeQGElcTRGYSVMKGYWNDPEKT----AEAIDA------DGWMHTGDLAVMDEEGYVNIVGRIKDMI 453
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 617 NVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWA 695
Cdd:PRK08315 454 IRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTeEDVRDFC----RGKIAHYKIPRYIRFV 529
|
170 180 190
....*....|....*....|....*....|
gi 378548265 696 PGLPKTRSGKIMRRILRKIAsrqLEELGDT 725
Cdd:PRK08315 530 DEFPMTVTGKIQKFKMREMM---IEELGLQ 556
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
198-708 |
1.57e-11 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 67.08 E-value: 1.57e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcn 277
Cdd:cd05914 8 LTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAIFV-- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivdaaldqSSKDGVSVgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaedplfL 357
Cdd:cd05914 86 ---------------------SDEDDVAL--------------------------------------------------I 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTtgGYMIYTATTFKYAFD-YKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVV----------- 425
Cdd:cd05914 95 NYTSGTTGNSKGVMLT--YRNIVSNVDGVKEVVlLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFldkipsakiia 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 426 ---FEGAPNYPDPgRCWDIVDKYKVSIFytaPTLVRSLMR-------DDDKFVTRHSRKSLRVLG-------SVGEPINP 488
Cdd:cd05914 173 lafAQVTPTLGVP-VPLVIEKIFKMDII---PKLTLKKFKfklakkiNNRKIRKLAFKKVHEAFGgnikefvIGGAKINP 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 489 SAWRwFFNVVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGE--CSGYLCVKGSW 566
Cdd:cd05914 249 DVEE-FLRTIG---FPYTIGYGMTETAPIISYSPPNR--IRLGSAGKVIDGVEVRIDSPDPATGEGEiiVRGPNVMKGYY 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 567 --PGAFRTLFgDHEryettyfkpfaGYYFSGDGCSRDKDGYYWLTGRVDDVI-NVSGHRIGTAEVESALVLHPQCAEAAV 643
Cdd:cd05914 323 knPEATAEAF-DKD-----------GWFHTGDLGKIDAEGYLYIRGRKKEMIvLSSGKNIYPEEIEAKINNMPFVLESLV 390
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 644 VgiEHEVKGQGI------YAFVTLLEGVPYSEELRKSLVLMVRNQIGAFA--APDRIHWAPgLPKTRSGKIMR 708
Cdd:cd05914 391 V--VQEKKLVALayidpdFLDVKALKQRNIIDAIKWEVRDKVNQKVPNYKkiSKVKIVKEE-FEKTPKGKIKR 460
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
197-711 |
2.18e-11 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 67.89 E-value: 2.18e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTc 276
Cdd:PRK05691 2213 TLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEYPLERLHYMIEDSGIGLLLS- 2291
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navkrgpktinlkaivDAALDQSS---KDGVSVGiCLTYDnslattrentkwqngrdvwwQDVISQYPTSCEVEWVDAED 353
Cdd:PRK05691 2292 ----------------DRALFEALgelPAGVARW-CLEDD--------------------AAALAAYSDAPLPFLSLPQH 2334
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKyAFDYKSTDV----YWCTADCGwitghSYVTYGPMLNGATVVV-FEG 428
Cdd:PRK05691 2335 QAYLIYTSGSTGKPKGVVVSHGEIAMHCQAVIE-RFGMRADDCelhfYSINFDAA-----SERLLVPLLCGARVVLrAQG 2408
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 429 ApnypdpgrcWD------IVDKYKVSIFYTAPTLVRSLMRdddKFVTRHSRKSLRVLGSVGEPINPSAWR---------W 493
Cdd:PRK05691 2409 Q---------WGaeeicqLIREQQVSILGFTPSYGSQLAQ---WLAGQGEQLPVRMCITGGEALTGEHLQrirqafapqL 2476
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FFNVVGdsrcpisdtwwQTETggfMITPLPGAWPQ--KPGSATFPFFGV----QPVIVDEKGNEIEGECSGYLCVKGSwp 567
Cdd:PRK05691 2477 FFNAYG-----------PTET---VVMPLACLAPEqlEEGAASVPIGRVvgarVAYILDADLALVPQGATGELYVGGA-- 2540
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 GAFRtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCA 639
Cdd:PRK05691 2541 GLAQ---GYHDRPGLTAERfvadPFAAdggrLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVR 2617
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 640 EAAVVGIEHEVKGQGIYAFVTLLEGVPYSE--ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK05691 2618 EAVVLALDTPSGKQLAGYLVSAVAGQDDEAqaALREALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
198-417 |
6.62e-10 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 62.69 E-value: 6.62e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSL--AQRIVDCKpnVILt 275
Cdd:PTZ00216 122 ITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALayALRETECK--AIV- 198
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 CNAvKRGPKTINLkaivdaaldqsSKDGVSVGICLTYDNSLAttrENTKWQNGRDVWWQDVI-------SQYP-TSCEve 347
Cdd:PTZ00216 199 CNG-KNVPNLLRL-----------MKSGGMPNTTIIYLDSLP---ASVDTEGCRLVAWTDVVakghsagSHHPlNIPE-- 261
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 wvDAEDPLFLLYTSGSTGKPKGVLHTTGgymiyTATTFKYAFDYKSTDVYWCTADcgwitGHSYVTYGPM 417
Cdd:PTZ00216 262 --NNDDLALIMYTSGTTGDPKGVMHTHG-----SLTAGILALEDRLNDLIGPPEE-----DETYCSYLPL 319
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
350-711 |
2.33e-09 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 60.11 E-value: 2.33e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGGYM-IYTATTFKYAFDYKSTDVYWCTAdcgwitghSYV-------TYGPMLNGA 421
Cdd:cd17648 92 NSTDLAYAIYTSGTTGKPKGVLVEHGSVVnLRTSLSERYFGRDNGDEAVLFFS--------NYVfdffveqMTLALLNGQ 163
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 422 TVVVFEGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRslMRDDDkfvtrhSRKSLRVLGSVGEPINPSAwrwFFNVVGDS 501
Cdd:cd17648 164 KLVVPPDEMRF-DPDRFYAYINREKVTYLSGTPSVLQ--QYDLA------RLPHLKRVDAAGEEFTAPV---FEKLRSRF 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 502 RCPISDTWWQTETGGF-MITPLPGAwPQKPGSATFPFFGV---------QPVIVDEKGN-EIEGEC--SGY-----LCVK 563
Cdd:cd17648 232 AGLIINAYGPTETTVTnHKRFFPGD-QRFDKSLGRPVRNTkcyvlndamKRVPVGAVGElYLGGDGvaRGYlnrpeLTAE 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 564 GSWPGAFRTlfgDHERYETTYfkpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAV 643
Cdd:cd17648 311 RFLPNPFQT---EQERARGRN----ARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAV 383
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 644 VGIEHEVKGQG-----IYAFVTLLEGVPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17648 384 VAKEDASQAQSriqkyLVGYYLPEPGHVPESDLLSFL----RAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
198-738 |
4.12e-09 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 59.68 E-value: 4.12e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILtcn 277
Cdd:cd17640 6 ITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALV--- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivdaaLDQSSKDgvsvgicltydnsLATtrentkwqngrdvwwqdvisqyptscevewvdaedplfL 357
Cdd:cd17640 83 ------------------VENDSDD-------------LAT--------------------------------------I 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCgWitgHSY---VTYGPMLNGATVVvFEGAPNYPD 434
Cdd:cd17640 94 IYTSGTTGNPKGVMLTHAN-LLHQIRSLSDIVPPQPGDRFLSILPI-W---HSYersAEYFIFACGCSQA-YTSIRTLKD 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 435 pgrcwDIvDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLG---SVGE---PIN-----PSAWRWFFNVVGdsrC 503
Cdd:cd17640 168 -----DL-KRVKPHYIVSVPRLWESLYSGIQKQVSKSSPIKQFLFLfflSGGIfkfGISgggalPPHVDTFFEAIG---I 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTETGGFMITPLPgaWPQKPGSATFPFFGVQPVIVDEKGNEI-EGECSGYLCVKGswPGAFRTLFGDHEryET 582
Cdd:cd17640 239 EVLNGYGLTETSPVVSARRL--KCNVRGSVGRPLPGTEIKIVDPEGNVVlPPGEKGIVWVRG--PQVMKGYYKNPE--AT 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 583 TYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVS-GHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIyafvtl 661
Cdd:cd17640 313 SKVLDSDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIEEALMRSPFIEQIMVVGQDQKRLGALI------ 386
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 378548265 662 legVPYSEELRKSLvlmvrNQIGAFAAPDRIHWAPGLPKTR--SGKIMRRILRKIASRQLEELGDTSTLADPSVVDQLI 738
Cdd:cd17640 387 ---VPNFEELEKWA-----KESGVKLANDRSQLLASKKVLKlyKNEIKDEISNRPGFKSFEQIAPFALLEEPFIENGEM 457
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
585-720 |
4.13e-09 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 59.62 E-value: 4.13e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 585 FKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEG 664
Cdd:PRK07445 319 ILDSQGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDP 398
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 665 VPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLE 720
Cdd:PRK07445 399 SISLEELKTAI----KDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQIAVQRLG 450
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
198-673 |
4.38e-09 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 59.54 E-value: 4.38e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcn 277
Cdd:cd17639 6 MSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIHSLNETECSAIFT-- 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivdaaldqsskdgvsvgicltyDNSlattrentkwqngrdvwwqdvisqyptscevewvdAEDPLFL 357
Cdd:cd17639 84 -----------------------------------DGK-----------------------------------PDDLACI 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGyMIYTATTFkyafdykstdvywctadCGWITGH-----SYVTYGP-------------MLN 419
Cdd:cd17639 94 MYTSGSTGNPKGVMLTHGN-LVAGIAGL-----------------GDRVPELlgpddRYLAYLPlahifelaaenvcLYR 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATV-------------------------VVFEGAPnypdpgRCWDIVDK-----------YKVSIFYTAPTLVRSLMRD 463
Cdd:cd17639 156 GGTIgygsprtltdkskrgckgdltefkpTLMVGVP------AIWDTIRKgvlaklnpmggLKRTLFWTAYQSKLKALKE 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 464 D------DKFVTRHSRKS----LRVLGSVGEPINPSAWRwFFNVVGdsrCPISDTWWQTETGGFMITPLPGAWpqKPGSA 533
Cdd:cd17639 230 GpgtpllDELVFKKVRAAlggrLRYMLSGGAPLSADTQE-FLNIVL---CPVIQGYGLTETCAGGTVQDPGDL--ETGRV 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 534 TFPFFGVQPVIVD-EKGNEI--EGECSGYLCVKGswPGAFRTLFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTG 610
Cdd:cd17639 304 GPPLPCCEIKLVDwEEGGYStdKPPPRGEILIRG--PNVFKGYYKNPEKTKEAFDG--DGWFHTGDIGEFHPDGTLKIID 379
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 611 RVDD-VINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKgqgIYAFVtllegVPYSEELRK 673
Cdd:cd17639 380 RKKDlVKLQNGEYIALEKLESIYRSNPLVNNICVYADPDKSY---PVAIV-----VPNEKHLTK 435
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
181-646 |
4.78e-08 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 56.45 E-value: 4.78e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAI----HWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGD-AVVIYLPMLmELPIAMLACARIGAVHSVVFAG 255
Cdd:PRK09274 21 DQLAVavpgGRGADGKLAYDELSFAELDARSDAIAHGLNAAGIGRGMrAVLMVTPSL-EFFALTFALFKAGAVPVLVDPG 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 256 FSADSLAQRIVDCKPNVILTCnavkrgPKTINLKAIVDAALDqSSKDGVSVG-ICLTYDNSLATTRENTKwqngrdvwwq 334
Cdd:PRK09274 100 MGIKNLKQCLAEAQPDAFIGI------PKAHLARRLFGWGKP-SVRRLVTVGgRLLWGGTTLATLLRDGA---------- 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 335 dvisqyPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGgymIYTA--TTFKYAFDYKSTDVYWCTADCgwitghsYV 412
Cdd:PRK09274 163 ------AAPFPMADLAPDDMAAILFTSGSTGTPKGVVYTHG---MFEAqiEALREDYGIEPGEIDLPTFPL-------FA 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 413 TYGPMLnGATVVVFEGAPNYP---DPGRCWDIVDKYKVSIFYTAPTLVRSLmrdddkfvTRHSRKSLRVLGSV------G 483
Cdd:PRK09274 227 LFGPAL-GMTSVIPDMDPTRPatvDPAKLFAAIERYGVTNLFGSPALLERL--------GRYGEANGIKLPSLrrvisaG 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EPINPSAWRWF----------FNVVGDSRC-PIS---------DTWWQTETGGfmitplpGAWPQKPgsatfpffgVQPV 543
Cdd:PRK09274 298 APVPIAVIERFramlppdaeiLTPYGATEAlPISsiesreilfATRAATDNGA-------GICVGRP---------VDGV 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 ------IVDEKGNEIEG--ECS----GYLCVKGswP----------------------GAFRTLFGDheryettyfkpfA 589
Cdd:PRK09274 362 evriiaISDAPIPEWDDalRLAtgeiGEIVVAG--PmvtrsyynrpeatrlakipdgqGDVWHRMGD------------L 427
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 590 GYyfsgdgcsRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGI 646
Cdd:PRK09274 428 GY--------LDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALVGV 476
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
183-370 |
8.52e-08 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 55.72 E-value: 8.52e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 183 TAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVkKGDAVVIYLPMLMELPIAMLA--CARIGAVH-SVVFAGFSAD 259
Cdd:PRK05850 21 TFIDYEQDPAGVAETLTWSQLYRRTLNVAEELRRHGS-TGDRAVILAPQGLEYIVAFLGalQAGLIAVPlSVPQGGAHDE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTCNAVkrgpktinlkaIVDAALDQSSKDGVSVGICLTYDnSLattrentkwqngrdvwwqDVISq 339
Cdd:PRK05850 100 RVSAVLRDTSPSVVLTTSAV-----------VDDVTEYVAPQPGQSAPPVIEVD-LL------------------DLDS- 148
|
170 180 190
....*....|....*....|....*....|.
gi 378548265 340 yPTSCEVEWVDAEDPLFLLYTSGSTGKPKGV 370
Cdd:PRK05850 149 -PRGSDARPRDLPSTAYLQYTSGSTRTPAGV 178
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
351-646 |
2.52e-07 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 54.00 E-value: 2.52e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 AEDPLFLLYTSGSTGKPKGVLhttggymiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLnGATVVVFEGAP 430
Cdd:cd05910 84 ADEPAAILFTSGSTGTPKGVV--------YRHGTFAAQIDALRQLYGIRPGEVDLATFPLFALFGPAL-GLTSVIPDMDP 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 431 NYP---DPGRCWDIVDKYKVSIFYTAPTLVRSLMRdddkFVTRHSRK--SLRVLGSVGEPINP-SAWRW---------FF 495
Cdd:cd05910 155 TRParaDPQKLVGAIRQYGVSIVFGSPALLERVAR----YCAQHGITlpSLRRVLSAGAPVPIaLAARLrkmlsdeaeIL 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 496 NVVGDSRC-PIS---DTWWQTETggfmiTPLPgawpqKPGSAT---FPFFGVQPVIV---DEKGNEIEGECS------GY 559
Cdd:cd05910 231 TPYGATEAlPVSsigSRELLATT-----TAAT-----SGGAGTcvgRPIPGVRVRIIeidDEPIAEWDDTLElprgeiGE 300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 560 LCVKGswPGAFRTLfgdHERYETTYFK--PFAGYYF---SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVL 634
Cdd:cd05910 301 ITVTG--PTVTPTY---VNRPVATALAkiDDNSEGFwhrMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNT 375
|
330
....*....|..
gi 378548265 635 HPQCAEAAVVGI 646
Cdd:cd05910 376 HPGVRRSALVGV 387
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
199-719 |
4.67e-07 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 53.08 E-value: 4.67e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMEL-PIAMLACARIGAV-------HSVVFAGFSADSLAqrivdckp 270
Cdd:PRK07768 31 TWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIaPTAQGLWMRGASLtmlhqptPRTDLAVWAEDTLR-------- 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 271 nvilTCNAVkrGPKTINLKAIVDAALDQSSKDGVSVgicLTYDNSLAttrentkwqngrdvwwqdvisqyPTSCEVEWVD 350
Cdd:PRK07768 103 ----VIGMI--GAKAVVVGEPFLAAAPVLEEKGIRV---LTVADLLA-----------------------ADPIDPVETG 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 AEDPLFLLYTSGSTGKPKGVLHTTG-----GYMIYTATTFKYAFDYKstdVYW--CTADCGWItghSYVTYgPMLNGATV 423
Cdd:PRK07768 151 EDDLALMQLTSGSTGSPKAVQITHGnlyanAEAMFVAAEFDVETDVM---VSWlpLFHDMGMV---GFLTV-PMYFGAEL 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEGAPNYPDPGRCWDIVDKYKVSIfYTAPT-----LVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRWFFNVV 498
Cdd:PRK07768 224 VKVTPMDFLRDPLLWAELISKYRGTM-TAAPNfayalLARRLRRQAKP--GAFDLSSLRFALNGAEPIDPADVEDLLDAG 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 G----DSRCPISDTWWQTETGGFMITPLPG-----------------AWPQKPGSAT------FPFFGVQPVIVDEKGN- 550
Cdd:PRK07768 301 ArfglRPEAILPAYGMAEATLAVSFSPCGAglvvdevdadllaalrrAVPATKGNTRrlatlgPPLPGLEVRVVDEDGQv 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 551 ---------EIEGEC--SGYLCVKGswpgaFRTLFGDHeryettyfkpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVS 619
Cdd:PRK07768 381 lpprgvgviELRGESvtPGYLTMDG-----FIPAQDAD------------GWLDTGDLGYLTEEGEVVVCGRVKDVIIMA 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 620 GHRIGTAEVESALV----LHPQCAEAavVGIEHEVKGQGiyaFVTLLEGVPYSEE-----LRKSLVLMVRNQIGafAAPD 690
Cdd:PRK07768 444 GRNIYPTDIERAAArvegVRPGNAVA--VRLDAGHSREG---FAVAVESNAFEDPaevrrIRHQVAHEVVAEVG--VRPR 516
|
570 580 590
....*....|....*....|....*....|.
gi 378548265 691 RIH-WAPG-LPKTRSGKimrriLRKIASRQL 719
Cdd:PRK07768 517 NVVvLGPGsIPKTPSGK-----LRRANAAEL 542
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
181-370 |
6.06e-07 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 52.59 E-value: 6.06e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGavHSVVFAGFSadS 260
Cdd:PRK04813 17 DFPAYDYLG------EKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAG--HAYIPVDVS--S 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVD----CKPNVILtcnavkrgpktinlkAIVDAALDQsskDGVSVgicLTYDNSlattrentkwqngrdvwwQDV 336
Cdd:PRK04813 87 PAERIEMiievAKPSLII---------------ATEELPLEI---LGIPV---ITLDEL------------------KDI 127
|
170 180 190
....*....|....*....|....*....|....
gi 378548265 337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGV 370
Cdd:PRK04813 128 FATGNPYDFDHAVKGDDNYYIIFTSGTTGKPKGV 161
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
593-727 |
8.57e-06 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 48.88 E-value: 8.57e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPySEELR 672
Cdd:PRK08308 294 FTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHEEID-PVQLR 372
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 673 KslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKImrrilrkiaSRQLEELGDTST 727
Cdd:PRK08308 373 E----WCIQHLAPYQVPHEIESVTEIPKNANGKV---------SRKLLELGEVTA 414
|
|
| PRK09188 |
PRK09188 |
serine/threonine protein kinase; Provisional |
631-742 |
4.51e-05 |
|
serine/threonine protein kinase; Provisional
Pssm-ID: 236400 [Multi-domain] Cd Length: 365 Bit Score: 46.30 E-value: 4.51e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 631 ALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVpYSEELRKSLVlmvrnQIGAFAAPDRIHWAPGLPKTRSGKIMRRI 710
Cdd:PRK09188 248 ALKSDPAVSDVAIALFSLPAKGVGLYAFVEAELPA-DEKSLRARLA-----GAKPPKPPEHIQPVAALPRDADGTVRDDI 321
|
90 100 110
....*....|....*....|....*....|..
gi 378548265 711 LRKIASRQLEELGDtsTLADPSVVDQLIALAD 742
Cdd:PRK09188 322 LRLIAMNQIDELDD--LLREPEIRGLVEAIAA 351
|
|
| alpha_am_amid |
TIGR03443 |
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ... |
197-371 |
7.03e-05 |
|
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.
Pssm-ID: 274582 [Multi-domain] Cd Length: 1389 Bit Score: 46.60 E-value: 7.03e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGF-------------------- 256
Cdd:TIGR03443 270 SFTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVIDPAYpparqtiylsvakpralivi 349
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 -SADSLAQRIVDCKPN---VILTCNAVkrgpKTINLKAIVDAALDQSSKDgvsvgiCLTYDNSLATTRENtkwqngrdvw 332
Cdd:TIGR03443 350 eKAGTLDQLVRDYIDKeleLRTEIPAL----ALQDDGSLVGGSLEGGETD------VLAPYQALKDTPTG---------- 409
|
170 180 190
....*....|....*....|....*....|....*....
gi 378548265 333 wqdVIsqyptscevewVDAEDPLFLLYTSGSTGKPKGVL 371
Cdd:TIGR03443 410 ---VV-----------VGPDSNPTLSFTSGSEGIPKGVL 434
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
197-252 |
3.48e-04 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 44.05 E-value: 3.48e-04
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVV 252
Cdd:cd17647 20 SFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVI 75
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
332-480 |
1.25e-03 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 42.11 E-value: 1.25e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 332 WWQDVISQYPTSCEVEWV---------DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYaFDYKSTDVYWCTAD 402
Cdd:PRK06334 154 FWEKCRIGIYMSIPFEWLmrwfgvsdkDPEDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKF-FSPKEDDVMMSFLP 232
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 403 CGWITGHSYVTYGPMLNGATvVVFEGAPNYPDpgRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLG 480
Cdd:PRK06334 233 PFHAYGFNSCTLFPLLSGVP-VVFAYNPLYPK--KIVEMIDEAKVTFLGSTPVFFDYILKTAKKQESCLPSLRFVVIG 307
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
359-397 |
5.92e-03 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 39.89 E-value: 5.92e-03
10 20 30 40
....*....|....*....|....*....|....*....|..
gi 378548265 359 YTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKS---TDVY 397
Cdd:cd05927 121 YTSGTTGNPKGVMLTHGNIVSNVAGVFKILEILNKinpTDVY 162
|
|
| PRK07868 |
PRK07868 |
acyl-CoA synthetase; Validated |
568-703 |
7.73e-03 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236121 [Multi-domain] Cd Length: 994 Bit Score: 39.70 E-value: 7.73e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 GAFRTLFGDHERYETTyfkpfaGYYFSgdgcsRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:PRK07868 825 SVKRGVFAPADTWIST------EYLFR-----RDDDGDYWLVDRRGSVIRTARGPVYTEPVTDALGRIGGVDLAVTYGVE 893
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQigafaAPDRIHWAPGLPKTRS 703
Cdd:PRK07868 894 VGGRQLAVAAVTLRPGAAITAADLTEALASLPVGL-----GPDIVHVVPEIPLSAT 944
|
|
|