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Conserved domains on  [gi|378548265|sp|B9DGD6|]
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RecName: Full=Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal; AltName: Full=Acetate--CoA ligase; AltName: Full=Acetyl-CoA synthetase; Flags: Precursor

Protein Classification

acetyl-CoA synthetase( domain architecture ID 11477027)

acetyl-coenzyme A synthetase catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PLN02654 PLN02654
acetate-CoA ligase
79-743 0e+00

acetate-CoA ligase


:

Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 1443.55  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  79 VLGESLASEENDLVFPSKEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKW-GDQVFSENLDVRKGPISIE 157
Cdd:PLN02654   1 VLGESLASEENDLVFPSKDFSAQALVSSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWeGDEVCSENLDVRKGPISIE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 158 WFKGGITNICYNCLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPI 237
Cdd:PLN02654  81 WFKGGKTNICYNCLDRNVEAGNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPI 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 238 AMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLA 317
Cdd:PLN02654 161 AMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVSVGICLTYENQLA 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 318 TTRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVY 397
Cdd:PLN02654 241 MKREDTKWQEGRDVWWQDVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVY 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 398 WCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLR 477
Cdd:PLN02654 321 WCTADCGWITGHSYVTYGPMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLR 400
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 478 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECS 557
Cdd:PLN02654 401 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECS 480
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 558 GYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 637
Cdd:PLN02654 481 GYLCVKKSWPGAFRTLYGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 560
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 638 CAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASR 717
Cdd:PLN02654 561 CAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASR 640
                        650       660
                 ....*....|....*....|....*.
gi 378548265 718 QLEELGDTSTLADPSVVDQLIALADV 743
Cdd:PLN02654 641 QLDELGDTSTLADPGVVDQLIALADS 666
 
Name Accession Description Interval E-value
PLN02654 PLN02654
acetate-CoA ligase
79-743 0e+00

acetate-CoA ligase


Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 1443.55  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  79 VLGESLASEENDLVFPSKEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKW-GDQVFSENLDVRKGPISIE 157
Cdd:PLN02654   1 VLGESLASEENDLVFPSKDFSAQALVSSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWeGDEVCSENLDVRKGPISIE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 158 WFKGGITNICYNCLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPI 237
Cdd:PLN02654  81 WFKGGKTNICYNCLDRNVEAGNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPI 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 238 AMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLA 317
Cdd:PLN02654 161 AMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVSVGICLTYENQLA 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 318 TTRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVY 397
Cdd:PLN02654 241 MKREDTKWQEGRDVWWQDVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVY 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 398 WCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLR 477
Cdd:PLN02654 321 WCTADCGWITGHSYVTYGPMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLR 400
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 478 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECS 557
Cdd:PLN02654 401 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECS 480
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 558 GYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 637
Cdd:PLN02654 481 GYLCVKKSWPGAFRTLYGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 560
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 638 CAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASR 717
Cdd:PLN02654 561 CAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASR 640
                        650       660
                 ....*....|....*....|....*.
gi 378548265 718 QLEELGDTSTLADPSVVDQLIALADV 743
Cdd:PLN02654 641 QLDELGDTSTLADPGVVDQLIALADS 666
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
108-730 0e+00

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 1171.57  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 108 QQYMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVfsenLDVRKGPISIEWFKGGITNICYNCLDKNVEAgLGDKTAIHW 187
Cdd:cd05966    1 EQYKELYKQSIEDPEEFWGEIAKELDWFKPW-DKV----LDWSKGPPFIKWFEGGKLNISYNCLDRHLKE-RGDKVAIIW 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 188 EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 267
Cdd:cd05966   75 EGDEPDQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRIND 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 268 CKPNVILTCNAVKRGPKTINLKAIVDAALDQsskdGVSVGICLTYDNslatTRENTKWQNGRDVWWQDVISQYPTSCEVE 347
Cdd:cd05966  155 AQCKLVITADGGYRGGKVIPLKEIVDEALEK----CPSVEKVLVVKR----TGGEVPMTEGRDLWWHDLMAKQSPECEPE 226
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 WVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFE 427
Cdd:cd05966  227 WMDSEDPLFILYTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVMFE 306
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 GAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISD 507
Cdd:cd05966  307 GTPTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPEAWMWYYEVIGKERCPIVD 386
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 508 TWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKP 587
Cdd:cd05966  387 TWWQTETGGIMITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGMARTIYGDHERYEDTYFSK 466
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPY 667
Cdd:cd05966  467 FPGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEP 546
                        570       580       590       600       610       620
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 668 SEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQlEELGDTSTLAD 730
Cdd:cd05966  547 SDELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAGE-EELGDTSTLAD 608
Ac_CoA_lig_AcsA TIGR02188
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ...
104-739 0e+00

acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.


Pssm-ID: 274022 [Multi-domain]  Cd Length: 626  Bit Score: 1061.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  104 VSSPQQYMEMHKRSMDDPAAFWSDIASE-FYWKQKWgDQVFSENLdvrkgPISIEWFKGGITNICYNCLDKNVEAgLGDK 182
Cdd:TIGR02188   1 IANLEQYKELYEESIEDPDKFWAKLARElLDWFKPF-TKVLDWSF-----PPFYKWFVGGELNVSYNCVDRHLEA-RPDK 73
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  183 TAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLA 262
Cdd:TIGR02188  74 VAIIWEGDEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEALA 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  263 QRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSskdGVSVGICLTYDNslaTTRENTKWQNGRDVWWQDVISQYPT 342
Cdd:TIGR02188 154 DRINDAGAKLVITADEGLRGGKVIPLKAIVDEALEKC---PVSVEHVLVVRR---TGNPVVPWVEGRDVWWHDLMAKASA 227
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  343 SCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGAT 422
Cdd:TIGR02188 228 YCEPEPMDSEDPLFILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCTADVGWITGHSYIVYGPLANGAT 307
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  423 VVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSR 502
Cdd:TIGR02188 308 TVMFEGVPTYPDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLGSVGEPINPEAWMWYYKVVGKER 387
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  503 CPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEG-ECSGYLCVKGSWPGAFRTLFGDHERYE 581
Cdd:TIGR02188 388 CPIVDTWWQTETGGIMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGpGEGGYLVIKQPWPGMLRTIYGDHERFV 467
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  582 TTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTL 661
Cdd:TIGR02188 468 DTYFSPFPGYYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTL 547
                         570       580       590       600       610       620       630
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265  662 LEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEELGDTSTLADPSVVDQLIA 739
Cdd:TIGR02188 548 KDGYEPDDELRKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEAEILGDTSTLEDPSVVEELIE 625
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
157-739 0e+00

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 890.62  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 157 EWFKGGITNICYNCLDKNVEaGLGDKTAIHWEGnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELP 236
Cdd:COG0365    1 RWFVGGRLNIAYNCLDRHAE-GRGDKVALIWEG-EDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAV 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 237 IAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSkdgvSVGICLTYDnsl 316
Cdd:COG0365   79 IAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEALEELP----SLEHVIVVG--- 151
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 317 attRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDV 396
Cdd:COG0365  152 ---RTGADVPMEGDLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDV 228
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 397 YWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSL 476
Cdd:COG0365  229 FWCTADIGWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSL 308
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 477 RVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPGaWPQKPGSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:COG0365  309 RLLGSAGEPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPVPGYDVAVVDEDGNPVPPGE 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:COG0365  385 EGELVIKGPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHP 464
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIAS 716
Cdd:COG0365  465 AVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAE 544
                        570       580
                 ....*....|....*....|...
gi 378548265 717 RqlEELGDTSTLADPSVVDQLIA 739
Cdd:COG0365  545 G--RPLGDTSTLEDPEALDEIKE 565
AMP-binding pfam00501
AMP-binding enzyme;
177-619 9.30e-113

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 347.76  E-value: 9.30e-113
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  177 AGLGDKTAIhwegnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGF 256
Cdd:pfam00501   6 ARTPDKTAL-----EVGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  257 SADSLAQRIVDCKPNVILTCNAVKrgpktinlkaIVDAALDQSSKDGVSVGICLTYDNSLATtrentkwqngrDVWWQDV 336
Cdd:pfam00501  81 PAEELAYILEDSGAKVLITDDALK----------LEELLEALGKLEVVKLVLVLDRDPVLKE-----------EPLPEEA 139
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTgGYMIYTATTFK----YAFDYKSTDVYWCTADCGWITGHSYV 412
Cdd:pfam00501 140 KPADVPPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIKrvrpRGFGLGPDDRVLSTLPLFHDFGLSLG 218
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  413 TYGPMLNGATVVVFEGAPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPINPSAWR 492
Cdd:pfam00501 219 LLGPLLAGATVVLPPGFPA-LDPAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLS--SLRLVLSGGAPLPPELAR 295
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  493 WFFNVVGdsrCPISDTWWQTETGGFMITPLPGAWPQ-KPGSATFPFFGVQPVIVDEK-GNEIEGECSGYLCVKGswPGAF 570
Cdd:pfam00501 296 RFRELFG---GALVNGYGLTETTGVVTTPLPLDEDLrSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRG--PGVM 370
                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*....
gi 378548265  571 RTLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVS 619
Cdd:pfam00501 371 KGYLNDPELTAEAFDED--GWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
 
Name Accession Description Interval E-value
PLN02654 PLN02654
acetate-CoA ligase
79-743 0e+00

acetate-CoA ligase


Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 1443.55  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  79 VLGESLASEENDLVFPSKEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKW-GDQVFSENLDVRKGPISIE 157
Cdd:PLN02654   1 VLGESLASEENDLVFPSKDFSAQALVSSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWeGDEVCSENLDVRKGPISIE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 158 WFKGGITNICYNCLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPI 237
Cdd:PLN02654  81 WFKGGKTNICYNCLDRNVEAGNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPI 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 238 AMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLA 317
Cdd:PLN02654 161 AMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVSVGICLTYENQLA 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 318 TTRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVY 397
Cdd:PLN02654 241 MKREDTKWQEGRDVWWQDVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVY 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 398 WCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLR 477
Cdd:PLN02654 321 WCTADCGWITGHSYVTYGPMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLR 400
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 478 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECS 557
Cdd:PLN02654 401 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECS 480
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 558 GYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 637
Cdd:PLN02654 481 GYLCVKKSWPGAFRTLYGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 560
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 638 CAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASR 717
Cdd:PLN02654 561 CAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASR 640
                        650       660
                 ....*....|....*....|....*.
gi 378548265 718 QLEELGDTSTLADPSVVDQLIALADV 743
Cdd:PLN02654 641 QLDELGDTSTLADPGVVDQLIALADS 666
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
108-730 0e+00

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 1171.57  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 108 QQYMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVfsenLDVRKGPISIEWFKGGITNICYNCLDKNVEAgLGDKTAIHW 187
Cdd:cd05966    1 EQYKELYKQSIEDPEEFWGEIAKELDWFKPW-DKV----LDWSKGPPFIKWFEGGKLNISYNCLDRHLKE-RGDKVAIIW 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 188 EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 267
Cdd:cd05966   75 EGDEPDQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRIND 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 268 CKPNVILTCNAVKRGPKTINLKAIVDAALDQsskdGVSVGICLTYDNslatTRENTKWQNGRDVWWQDVISQYPTSCEVE 347
Cdd:cd05966  155 AQCKLVITADGGYRGGKVIPLKEIVDEALEK----CPSVEKVLVVKR----TGGEVPMTEGRDLWWHDLMAKQSPECEPE 226
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 WVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFE 427
Cdd:cd05966  227 WMDSEDPLFILYTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVMFE 306
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 GAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISD 507
Cdd:cd05966  307 GTPTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPEAWMWYYEVIGKERCPIVD 386
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 508 TWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKP 587
Cdd:cd05966  387 TWWQTETGGIMITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGMARTIYGDHERYEDTYFSK 466
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPY 667
Cdd:cd05966  467 FPGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEP 546
                        570       580       590       600       610       620
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 668 SEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQlEELGDTSTLAD 730
Cdd:cd05966  547 SDELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAGE-EELGDTSTLAD 608
PRK00174 PRK00174
acetyl-CoA synthetase; Provisional
94-739 0e+00

acetyl-CoA synthetase; Provisional


Pssm-ID: 234677 [Multi-domain]  Cd Length: 637  Bit Score: 1137.56  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  94 PSKEFSGQALVSsPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVFSENldvrkgPISIEWFKGGITNICYNCLDK 173
Cdd:PRK00174   4 PPAEFAANALID-MEQYKALYQESVEDPEGFWAEQAKRLDWFKPF-DTVLDWN------APFIKWFEDGELNVSYNCLDR 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 174 NVEAGlGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVF 253
Cdd:PRK00174  76 HLKTR-GDKVAIIWEGDDPGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVVF 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 254 AGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDqsskdgvsvgICLTYDNSLATTR--ENTKWQNGRDV 331
Cdd:PRK00174 155 GGFSAEALADRIIDAGAKLVITADEGVRGGKPIPLKANVDEALA----------NCPSVEKVIVVRRtgGDVDWVEGRDL 224
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 332 WWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSY 411
Cdd:PRK00174 225 WWHELVAGASDECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWVTGHSY 304
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 412 VTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAW 491
Cdd:PRK00174 305 IVYGPLANGATTLMFEGVPNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKKYDLSSLRLLGSVGEPINPEAW 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 492 RWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFR 571
Cdd:PRK00174 385 EWYYKVVGGERCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDPWPGMMR 464
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 572 TLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVK 651
Cdd:PRK00174 465 TIYGDHERFVKTYFSTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIK 544
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 652 GQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQlEELGDTSTLADP 731
Cdd:PRK00174 545 GQGIYAFVTLKGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRILRKIAEGE-EILGDTSTLADP 623

                 ....*...
gi 378548265 732 SVVDQLIA 739
Cdd:PRK00174 624 SVVEKLIE 631
Ac_CoA_lig_AcsA TIGR02188
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ...
104-739 0e+00

acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.


Pssm-ID: 274022 [Multi-domain]  Cd Length: 626  Bit Score: 1061.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  104 VSSPQQYMEMHKRSMDDPAAFWSDIASE-FYWKQKWgDQVFSENLdvrkgPISIEWFKGGITNICYNCLDKNVEAgLGDK 182
Cdd:TIGR02188   1 IANLEQYKELYEESIEDPDKFWAKLARElLDWFKPF-TKVLDWSF-----PPFYKWFVGGELNVSYNCVDRHLEA-RPDK 73
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  183 TAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLA 262
Cdd:TIGR02188  74 VAIIWEGDEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEALA 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  263 QRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSskdGVSVGICLTYDNslaTTRENTKWQNGRDVWWQDVISQYPT 342
Cdd:TIGR02188 154 DRINDAGAKLVITADEGLRGGKVIPLKAIVDEALEKC---PVSVEHVLVVRR---TGNPVVPWVEGRDVWWHDLMAKASA 227
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  343 SCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGAT 422
Cdd:TIGR02188 228 YCEPEPMDSEDPLFILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCTADVGWITGHSYIVYGPLANGAT 307
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  423 VVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSR 502
Cdd:TIGR02188 308 TVMFEGVPTYPDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLGSVGEPINPEAWMWYYKVVGKER 387
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  503 CPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEG-ECSGYLCVKGSWPGAFRTLFGDHERYE 581
Cdd:TIGR02188 388 CPIVDTWWQTETGGIMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGpGEGGYLVIKQPWPGMLRTIYGDHERFV 467
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  582 TTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTL 661
Cdd:TIGR02188 468 DTYFSPFPGYYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTL 547
                         570       580       590       600       610       620       630
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265  662 LEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEELGDTSTLADPSVVDQLIA 739
Cdd:TIGR02188 548 KDGYEPDDELRKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEAEILGDTSTLEDPSVVEELIE 625
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
157-739 0e+00

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 890.62  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 157 EWFKGGITNICYNCLDKNVEaGLGDKTAIHWEGnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELP 236
Cdd:COG0365    1 RWFVGGRLNIAYNCLDRHAE-GRGDKVALIWEG-EDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAV 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 237 IAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSkdgvSVGICLTYDnsl 316
Cdd:COG0365   79 IAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEALEELP----SLEHVIVVG--- 151
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 317 attRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDV 396
Cdd:COG0365  152 ---RTGADVPMEGDLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDV 228
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 397 YWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSL 476
Cdd:COG0365  229 FWCTADIGWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSL 308
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 477 RVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPGaWPQKPGSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:COG0365  309 RLLGSAGEPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPVPGYDVAVVDEDGNPVPPGE 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:COG0365  385 EGELVIKGPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHP 464
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIAS 716
Cdd:COG0365  465 AVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAE 544
                        570       580
                 ....*....|....*....|...
gi 378548265 717 RqlEELGDTSTLADPSVVDQLIA 739
Cdd:COG0365  545 G--RPLGDTSTLEDPEALDEIKE 565
ACS-like cd17634
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ...
110-707 0e+00

acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341289 [Multi-domain]  Cd Length: 587  Bit Score: 722.44  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 110 YMEMHKRSMDDPAAFWSDIASEFYWKqkWGDQVfSENLDVRKGPISIEWFKGGITNICYNCLDKNVEAGlGDKTAIHWEG 189
Cdd:cd17634    1 YETKYRQSINDPDTFWGEAGKILDWI--TPYQK-VKNTSFAPGAPSIKWFEDATLNLAANALDRHLREN-GDRTAIIYEG 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 190 NELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:cd17634   77 DDTSQSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSS 156
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAVKRGPKTINLKAIVDAALDQSskdGVSVGICLTYDNslatTRENTKWQNGRDVWWQDVISQYPTSCEVEWV 349
Cdd:cd17634  157 SRLLITADGGVRAGRSVPLKKNVDDALNPN---VTSVEHVIVLKR----TGSDIDWQEGRDLWWRDLIAKASPEHQPEAM 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGA 429
Cdd:cd17634  230 NAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACGATTLLYEGV 309
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 PNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTW 509
Cdd:cd17634  310 PNWPTPARMWQVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSLRILGSVGEPINPEAYEWYWKKIGKEKCPVVDTW 389
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 510 WQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFA 589
Cdd:cd17634  390 WQTETGGFMITPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQTRTLFGDHERFEQTYFSTFK 469
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 590 GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSE 669
Cdd:cd17634  470 GMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSP 549
                        570       580       590
                 ....*....|....*....|....*....|....*...
gi 378548265 670 ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIM 707
Cdd:cd17634  550 ELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKIM 587
PrpE cd05967
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ...
110-737 0e+00

Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.


Pssm-ID: 341271 [Multi-domain]  Cd Length: 617  Bit Score: 645.53  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 110 YMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVFSenldvRKGPISIEWFKGGITNICYNCLDKNVEAGLGDKTAIHWEG 189
Cdd:cd05967    1 YEEVYARSIAEPEAFWAEQARLIDWFKPP-EKILD-----NSNPPFTRWFVGGRLNTCYNALDRHVEAGRGDQIALIYDS 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 190 NELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:cd05967   75 PVTGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDAK 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVgicLTYDNSLATTRENTKwqnGRDVWWQDVISQYpTSCEVEWV 349
Cdd:cd05967  155 PKLIVTASCGIEPGKVVPYKPLLDKALELSGHKPHHV---LVLNRPQVPADLTKP---GRDLDWSELLAKA-EPVDCVPV 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGA 429
Cdd:cd05967  228 AATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWAASDVGWVVGHSYIVYGPLLHGATTVLYEGK 307
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 P-NYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDD--KFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPIS 506
Cdd:cd05967  308 PvGTPDPGAFWRVIEKYQVNALFTAPTAIRAIRKEDPdgKYIKKYDLSSLRTLFLAGERLDPPTLEWAENTLG---VPVI 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 507 DTWWQTETGGFMITPLPG--AWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSW-PGAFRTLFGDHERYETT 583
Cdd:cd05967  385 DHWWQTETGWPITANPVGlePLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLPLpPGCLLTLWKNDERFKKL 464
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 584 YFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLE 663
Cdd:cd05967  465 YLSKFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKE 544
                        570       580       590       600       610       620       630
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 664 GVPYS-EELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIAsrQLEELGDTSTLADPSVVDQL 737
Cdd:cd05967  545 GVKITaEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIA--DGEDYTIPSTIEDPSVLDEI 617
prpE PRK10524
propionyl-CoA synthetase; Provisional
108-737 0e+00

propionyl-CoA synthetase; Provisional


Pssm-ID: 182517 [Multi-domain]  Cd Length: 629  Bit Score: 581.52  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 108 QQYMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVfsenLDVRKGPISiEWFKGGITNICYNCLDKNVEAGlGDKTAIHW 187
Cdd:PRK10524   2 MSYSEFYQRSIDDPEAFWAEQARRIDWQTPF-TQV----LDYSNPPFA-RWFVGGRTNLCHNAVDRHLAKR-PEQLALIA 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 188 EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 267
Cdd:PRK10524  75 VSTETDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDD 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 268 CKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVgicLTYDNSLATTRentkWQNGRDVWWQDVISQY-PTSCEV 346
Cdd:PRK10524 155 AKPVLIVSADAGSRGGKVVPYKPLLDEAIALAQHKPRHV---LLVDRGLAPMA----RVAGRDVDYATLRAQHlGARVPV 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 347 EWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVF 426
Cdd:PRK10524 228 EWLESNEPSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETFFCASDIGWVVGHSYIVYAPLLAGMATIMY 307
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 EGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPIS 506
Cdd:PRK10524 308 EGLPTRPDAGIWWRIVEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSSLRALFLAGEPLDEPTASWISEALG---VPVI 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 507 DTWWQTETGGFMITPLPG--AWPQKPGSATFPFFGVQPVIVDEK-GNEIEGECSGYLCVKGSW-PGAFRTLFGDHERYET 582
Cdd:PRK10524 385 DNYWQTETGWPILAIARGveDRPTRLGSPGVPMYGYNVKLLNEVtGEPCGPNEKGVLVIEGPLpPGCMQTVWGDDDRFVK 464
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 583 TYFKPFAG-YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTL 661
Cdd:PRK10524 465 TYWSLFGRqVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVP 544
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 662 LEGVPY-SEELRKSL--VLM--VRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQleELGDTSTLADPSVVDQ 736
Cdd:PRK10524 545 KDSDSLaDREARLALekEIMalVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQAIAEGR--DPGDLTTIEDPAALQQ 622

                 .
gi 378548265 737 L 737
Cdd:PRK10524 623 I 623
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
108-731 2.06e-174

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 513.19  E-value: 2.06e-174
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 108 QQYMEMHKRSMDDPAAFWSDIASEF-YWKQKWGDQVfsenLDVRKGPISIEWFKGGITNICYNCLDKNVeAGLGDKTAIH 186
Cdd:cd05968    7 PDLEAFLERSAEDNAWFWGEFVKDVgIEWYEPPYQT----LDLSGGKPWAAWFVGGRMNIVEQLLDKWL-ADTRTRPALR 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 187 WEGnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIV 266
Cdd:cd05968   82 WEG-EDGTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQ 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 267 DCKPNVILTCNAVKRGPKTINLKAIVDAALDQsskdgvsvgiCLTYDNSLATTR--ENTKWQNGRDVWWQDVISQYPTsc 344
Cdd:cd05968  161 DAEAKALITADGFTRRGREVNLKEEADKACAQ----------CPTVEKVVVVRHlgNDFTPAKGRDLSYDEEKETAGD-- 228
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 345 EVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTD-VYWCTaDCGWITGhSYVTYGPMLNGATV 423
Cdd:cd05968  229 GAERTESEDPLMIIYTSGTTGKPKGTVHVHAGFPLKAAQDMYFQFDLKPGDlLTWFT-DLGWMMG-PWLIFGGLILGATM 306
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRC 503
Cdd:cd05968  307 VLYDGAPDHPKADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLRVLGSTGEPWNPEPWNWLFETVGKGRN 386
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTET-----GGFMITPLpgawpqKPGSATFPFFGVQPVIVDEKGNEIEGEcSGYLCVKGSWPGAFRTLFGDHE 578
Cdd:cd05968  387 PIINYSGGTEIsggilGNVLIKPI------KPSSFNGPVPGMKADVLDESGKPARPE-VGELVLLAPWPGMTRGFWRDED 459
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 579 RYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAF 658
Cdd:cd05968  460 RYLETYWSRFDNVWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCF 539
                        570       580       590       600       610       620       630
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 659 VTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRkiASRQLEELGDTSTLADP 731
Cdd:cd05968  540 VVLKPGVTPTEALAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIR--AAYLGKELGDLSSLENP 610
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
148-730 9.51e-174

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 510.21  E-value: 9.51e-174
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 148 DVRKgpiSIEWFKGGITNICYNCLDKNVEAGLGDKTAIHWEGNELGVDasLTYSELLQRVCQLANYLKDNGVKKGDAVVI 227
Cdd:PRK04319  29 EVEK---EFSWLETGKVNIAYEAIDRHADGGRKDKVALRYLDASRKEK--YTYKELKELSNKFANVLKELGVEKGDRVFI 103
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 228 YLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAV---KRGPKTINLKAIVDAALDQSSKDGv 304
Cdd:PRK04319 104 FMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALlerKPADDLPSLKHVLLVGEDVEEGPG- 182
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 305 svgiCLTYDNSLAttrentkwqngrdvwwqdvisQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATT 384
Cdd:PRK04319 183 ----TLDFNALME---------------------QASDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHNA-MLQHYQT 236
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 385 FKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDD 464
Cdd:PRK04319 237 GKYVLDLHEDDVYWCTADPGWVTGTSYGIFAPWLNGATNVIDGGRF---SPERWYRILEDYKVTVWYTAPTAIRMLMGAG 313
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 465 DKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPgAWPQKPGSATFPFFGVQPVI 544
Cdd:PRK04319 314 DDLVKKYDLSSLRHILSVGEPLNPEVVRWGMKVFG---LPIHDNWWMTETGGIMIANYP-AMDIKPGSMGKPLPGIEAAI 389
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 545 VDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETtYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIG 624
Cdd:PRK04319 390 VDDQGNELPPNRMGNLAIKKGWPSMMRGIWNNPEKYES-YFAG--DWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVG 466
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 625 TAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSG 704
Cdd:PRK04319 467 PFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSG 546
                        570       580
                 ....*....|....*....|....*..
gi 378548265 705 KIMRRILRkiaSRQLE-ELGDTSTLAD 730
Cdd:PRK04319 547 KIMRRVLK---AWELGlPEGDLSTMED 570
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
198-716 2.09e-142

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 424.99  E-value: 2.09e-142
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCN 277
Cdd:cd05969    1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AVKRgpKTinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDPLFL 357
Cdd:cd05969   81 ELYE--RT----------------------------------------------------------------DPEDPTLL 94
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPnypDPGR 437
Cdd:cd05969   95 HYTSGTTGTPKGVLHVHDA-MIFYYFTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEGRF---DAES 170
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 438 CWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGF 517
Cdd:cd05969  171 WYGIIERVKVTVWYTAPTAIRMLMKEGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFG---VPIHDTWWQTETGSI 247
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 518 MITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETtYFKpfAGYYFSGDG 597
Cdd:cd05969  248 MIANYPCM-PIKPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGWPSMFRGIWNDEERYKN-SFI--DGWYLTGDL 323
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 598 CSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVL 677
Cdd:cd05969  324 AYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIIN 403
                        490       500       510
                 ....*....|....*....|....*....|....*....
gi 378548265 678 MVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIAS 716
Cdd:cd05969  404 FVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLKAKEL 442
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
198-713 2.75e-117

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 359.73  E-value: 2.75e-117
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIvdckpnviltcn 277
Cdd:cd05972    1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRL------------ 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpKTINLKAIVdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewVDAEDPLFL 357
Cdd:cd05972   69 ------EAAGAKAIV--------------------------------------------------------TDAEDPALI 86
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGgYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNypDPGR 437
Cdd:cd05972   87 YFTSGTTGLPKGVLHTHS-YPLGHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRF--DAER 163
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 438 CWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGgF 517
Cdd:cd05972  164 ILELLERYGVTSFCGPPTAYRMLIKQD---LSSYKFSHLRLVVSAGEPLNPEVIEWWRAATG---LPIRDGYGQTETG-L 236
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 518 MITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFkpfAGYYFSGDG 597
Cdd:cd05972  237 TVGNFPDM-PVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPPGLFLGYVGDPEKTEASIR---GDYYLTGDR 312
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 598 CSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVL 677
Cdd:cd05972  313 AYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQG 392
                        490       500       510
                 ....*....|....*....|....*....|....*.
gi 378548265 678 MVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05972  393 HVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
AMP-binding pfam00501
AMP-binding enzyme;
177-619 9.30e-113

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 347.76  E-value: 9.30e-113
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  177 AGLGDKTAIhwegnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGF 256
Cdd:pfam00501   6 ARTPDKTAL-----EVGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  257 SADSLAQRIVDCKPNVILTCNAVKrgpktinlkaIVDAALDQSSKDGVSVGICLTYDNSLATtrentkwqngrDVWWQDV 336
Cdd:pfam00501  81 PAEELAYILEDSGAKVLITDDALK----------LEELLEALGKLEVVKLVLVLDRDPVLKE-----------EPLPEEA 139
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTgGYMIYTATTFK----YAFDYKSTDVYWCTADCGWITGHSYV 412
Cdd:pfam00501 140 KPADVPPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIKrvrpRGFGLGPDDRVLSTLPLFHDFGLSLG 218
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  413 TYGPMLNGATVVVFEGAPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPINPSAWR 492
Cdd:pfam00501 219 LLGPLLAGATVVLPPGFPA-LDPAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLS--SLRLVLSGGAPLPPELAR 295
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  493 WFFNVVGdsrCPISDTWWQTETGGFMITPLPGAWPQ-KPGSATFPFFGVQPVIVDEK-GNEIEGECSGYLCVKGswPGAF 570
Cdd:pfam00501 296 RFRELFG---GALVNGYGLTETTGVVTTPLPLDEDLrSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRG--PGVM 370
                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*....
gi 378548265  571 RTLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVS 619
Cdd:pfam00501 371 KGYLNDPELTAEAFDED--GWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
180-721 5.00e-97

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 307.89  E-value: 5.00e-97
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:COG0318   13 PDRPALVFGG------RRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPRLTAE 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTCnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisq 339
Cdd:COG0318   87 ELAYILEDSGARALVTA--------------------------------------------------------------- 103
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 yptscevewvdaedplFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLN 419
Cdd:COG0318  104 ----------------LILYTSGTTGRPKGVMLTHRN-LLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLA 166
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVFEGapnyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRWFFNVVG 499
Cdd:COG0318  167 GATLVLLPR----FDPERVLELIERERVTVLFGVPTMLARLLRHPEF--ARYDLSSLRLVVSGGAPLPPELLERFEERFG 240
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dsrCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEI----EGEcsgyLCVKGswPGAFRTLFG 575
Cdd:COG0318  241 ---VRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPGVEVRIVDEDGRELppgeVGE----IVVRG--PNVMKGYWN 311
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHEryETTyfKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQG 654
Cdd:COG0318  312 DPE--ATA--EAFRdGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGER 387
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 655 IYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEE 721
Cdd:COG0318  388 VVAFVVLRPGAELDaEELRAFL----RERLARYKVPRRVEFVDELPRTASGKIDRRALRERYAAGALE 451
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
353-707 9.85e-89

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 282.25  E-value: 9.85e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYaFDYKSTDVYWCTADCGWItGHSYVTYGPMLNGATVVVFEGapny 432
Cdd:cd04433    1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAAS-GGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPK---- 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 433 PDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQT 512
Cdd:cd04433   75 FDPEAALELIEREKVTILLGVPTLLARLLKAPES--AGYDLSSLRALVSGGAPLPPELLERFEEAPG---IKLVNGYGLT 149
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 513 ETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLfgdheRYETTYFKPFAGYY 592
Cdd:cd04433  150 ETGGTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWN-----NPEATAAVDEDGWY 224
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEEL 671
Cdd:cd04433  225 RTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGAdLDAEEL 304
                        330       340       350
                 ....*....|....*....|....*....|....*.
gi 378548265 672 RKSlvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIM 707
Cdd:cd04433  305 RAH----VRERLAPYKVPRRVVFVDALPRTASGKID 336
AACS cd05943
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ...
110-735 6.04e-87

Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.


Pssm-ID: 341265 [Multi-domain]  Cd Length: 629  Bit Score: 286.86  E-value: 6.04e-87
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 110 YMEMHKRSMDDPAAFWSDIasefyWKqkWGDQVFS---ENLDVRKGPI-SIEWFKGGITNICYNCLDKnveAGLGDKTAI 185
Cdd:cd05943   19 YAALHRWSVDDPGAFWAAV-----WD--FSGVRGSkpyDVVVVSGRIMpGARWFPGARLNYAENLLRH---ADADDPAAI 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 186 HweGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRI 265
Cdd:cd05943   89 Y--AAEDGERTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRF 166
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 266 VDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWqngrdVWWQDVISQYPT-SC 344
Cdd:cd05943  167 GQIEPKVLFAVDAYTYNGKRHDVREKVAELVKGLPSLLAVVVVPYTVAAGQPDLSKIAKA-----LTLEDFLATGAAgEL 241
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 345 EVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYgpMLNGATVV 424
Cdd:cd05943  242 EFEPLPFDHPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHILHCDLRPGDRLFYYTTCGWMMWNWLVSG--LAVGATIV 319
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 425 VFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrcp 504
Cdd:cd05943  320 LYDGSPFYPDTNALWDLADEEGITVFGTSAKYLDALEKAGLKPAETHDLSSLRTILSTGSPLKPESFDYVYDHIK----- 394
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 iSDTWWQTETGG------FMIT-PLpgaWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKG--SWPGAFrtlFG 575
Cdd:cd05943  395 -PDVLLASISGGtdiiscFVGGnPL---LPVYRGEIQCRGLGMAVEAFDEEGKPVWGEKGELVCTKPfpSMPVGF---WN 467
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHE--RYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:cd05943  468 DPDgsRYRAAYFAKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDE 547
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 654 GIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRqlEELGDTSTLADPSV 733
Cdd:cd05943  548 RVILFVKLREGVELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGKKVEVAVKKIIAG--RPVKNAGALANPES 625

                 ..
gi 378548265 734 VD 735
Cdd:cd05943  626 LD 627
PTZ00237 PTZ00237
acetyl-CoA synthetase; Provisional
105-713 4.24e-80

acetyl-CoA synthetase; Provisional


Pssm-ID: 240325 [Multi-domain]  Cd Length: 647  Bit Score: 268.92  E-value: 4.24e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 105 SSPQQYMEMHKRSMDDPAAFWSDIASEF-YWkQKWGDQVFSENldvrkgPISIEWFKGGITNICYNCLDKNVEAGLG-DK 182
Cdd:PTZ00237   5 SDPFDYENDSNYANSNPESFWDEVAKKYvHW-DKMYDKVYSGD------EIYPDWFKGGELNTCYNVLDIHVKNPLKrDQ 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 183 TAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLA 262
Cdd:PTZ00237  78 DALIYECPYLKKTIKLTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSLI 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 263 QRIVDCKPNVILTCNAVKRGPKTI----NLKA-------------------IVDAALDQSSKDGVSVGICLTYDNSLATT 319
Cdd:PTZ00237 158 DRIETITPKLIITTNYGILNDEIItftpNLKEaielstfkpsnvitlfrndITSESDLKKIETIPTIPNTLSWYDEIKKI 237
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 320 RENTkwqngrdvwwQDVISQYPTscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWC 399
Cdd:PTZ00237 238 KENN----------QSPFYEYVP------VESSHPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFS 301
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 400 TADCGWITGHSYVtYGPMLNGATVVVFEGA----PNYPDpgRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSR-- 473
Cdd:PTZ00237 302 HSSIGWVSFHGFL-YGSLSLGNTFVMFEGGiiknKHIED--DLWNTIEKHKVTHTLTLPKTIRYLIKTDPEATIIRSKyd 378
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 474 -KSLRVLGSVGEPINPSAWRWFFNVVgdsRCPISDTWWQTETGgfmITPLPG-AWPQKPGSAT-FPFFGVQPVIVDEKGN 550
Cdd:PTZ00237 379 lSNLKEIWCGGEVIEESIPEYIENKL---KIKSSRGYGQTEIG---ITYLYCyGHINIPYNATgVPSIFIKPSILSEDGK 452
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 551 EIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVES 630
Cdd:PTZ00237 453 ELNVNEIGEVAFKLPMPPSFATTFYKNDEKFKQLFSKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIET 532
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 631 ALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSE----ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKI 706
Cdd:PTZ00237 533 SILKHPLVLECCSIGIYDPDCYNVPIGLLVLKQDQSNQSidlnKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKI 612

                 ....*..
gi 378548265 707 MRRILRK 713
Cdd:PTZ00237 613 PRQIISK 619
PRK03584 PRK03584
acetoacetate--CoA ligase;
110-741 3.75e-79

acetoacetate--CoA ligase;


Pssm-ID: 235134 [Multi-domain]  Cd Length: 655  Bit Score: 266.66  E-value: 3.75e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 110 YMEMHKRSMDDPAAFWSDIASEFYWKQKWGDQVFsenLDVRKGPISiEWFKGGITNICYNCLdknvEAGLGDKTAIHWEG 189
Cdd:PRK03584  36 YAALWRWSVEDLEAFWQSVWDFFGVIGSTPYTVV---LAGRRMPGA-RWFPGARLNYAENLL----RHRRDDRPAIIFRG 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 190 nELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:PRK03584 108 -EDGPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQGVLDRFGQIE 186
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAVKRGPKTINLKAIVDAALDQ--SSKDGVSVgiclTYDNSLATTRENTKWqngrdVWWQDVISQYPTS-CEV 346
Cdd:PRK03584 187 PKVLIAVDGYRYGGKAFDRRAKVAELRAAlpSLEHVVVV----PYLGPAAAAAALPGA-----LLWEDFLAPAEAAeLEF 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 347 EWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVY-WCTAdCGWITGHSYVtyGPMLNGATVVV 425
Cdd:PRK03584 258 EPVPFDHPLWILYSSGTTGLPKCIVHGHGGILLEHLKELGLHCDLGPGDRFfWYTT-CGWMMWNWLV--SGLLVGATLVL 334
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 426 FEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrcpi 505
Cdd:PRK03584 335 YDGSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDACEKAGLVPGETHDLSALRTIGSTGSPLPPEGFDWVYEHVK------ 408
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 506 SDTWWQTETGGfmiTPLPGAW-------PQKPGSATFPFFGVQPVIVDEKGNEIEGEcSGYLCVKGSWPgAFRTLF---G 575
Cdd:PRK03584 409 ADVWLASISGG---TDICSCFvggnpllPVYRGEIQCRGLGMAVEAWDEDGRPVVGE-VGELVCTKPFP-SMPLGFwndP 483
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK03584 484 DGSRYRDAYFDTFPGVWRHGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPEVLDSLVIGQEWPDGDVRM 563
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 656 YAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIM----RRILRKiasRQLEELGDTSTLADP 731
Cdd:PRK03584 564 PLFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKKVelpvKKLLHG---RPVKKAVNRDALANP 640
                        650
                 ....*....|
gi 378548265 732 SVVDQLIALA 741
Cdd:PRK03584 641 EALDWFADLA 650
MACS_like_2 cd05973
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
198-713 8.36e-79

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341277 [Multi-domain]  Cd Length: 437  Bit Score: 259.37  E-value: 8.36e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILtCN 277
Cdd:cd05973    1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVV-TD 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AVKRgpktinlkaivdAALDqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaEDPLFL 357
Cdd:cd05973   80 AANR------------HKLD------------------------------------------------------SDPFVM 93
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTT------GGYMiytattfKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPN 431
Cdd:cd05973   94 MFTSGTTGLPKGVPVPLralaafGAYL-------RDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEGGFS 166
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 432 YPDpgrCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRhSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQ 511
Cdd:cd05973  167 VES---TWRVIERLGVTNLAGSPTAYRLLMAAGAEVPAR-PKGRLRRVSSAGEPLTPEVIRWFDAALG---VPIHDHYGQ 239
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 512 TETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGS-----WpgafrtlFGDHERYETTyfK 586
Cdd:cd05973  240 TELGMVLANHHALEHPVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIDIAnsplmW-------FRGYQLPDTP--A 310
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 587 PFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVP 666
Cdd:cd05973  311 IDGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHE 390
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 378548265 667 YSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05973  391 GTPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
199-712 1.32e-74

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 248.50  E-value: 1.32e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcna 278
Cdd:cd05971    8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT--- 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 279 vkrgpktinlkaivdaaldqsskDGvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdAEDPLFLL 358
Cdd:cd05971   85 -----------------------DG-----------------------------------------------SDDPALII 94
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 359 YTSGSTGKPKGVLHTTGgYMIYTATTFKYAFDY--KSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNypDPG 436
Cdd:cd05971   95 YTSGTTGPPKGALHAHR-VLLGHLPGVQFPFNLfpRDGDLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRMTKF--DPK 171
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 437 RCWDIVDKYKVSIFYTAPTLVRsLMRDDDKfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDsrcPISDTWWQTEtGG 516
Cdd:cd05971  172 AALDLMSRYGVTTAFLPPTALK-MMRQQGE-QLKHAQVKLRAIATGGESLGEELLGWAREQFGV---EVNEFYGQTE-CN 245
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 517 FMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYEttyfKPFAGYYF-SG 595
Cdd:cd05971  246 LVIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDPVAFLGYWNNPSATE----KKMAGDWLlTG 321
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 596 DGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSL 675
Cdd:cd05971  322 DLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDALAREI 401
                        490       500       510
                 ....*....|....*....|....*....|....*..
gi 378548265 676 VLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05971  402 QELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
171-712 2.44e-67

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 230.72  E-value: 2.44e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVEAGLGDKTAIhwegneLGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHS 250
Cdd:cd05959    9 VDLNLNEGRGDKTAF------IDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPV 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 251 VVFAGFSADSLAQRIVDCKPNVILTCNAvkrgpktinLKAIVDAALDQSSKDGVSVGICltydnslattreNTKWQNGRD 330
Cdd:cd05959   83 PVNTLLTPDDYAYYLEDSRARVVVVSGE---------LAPVLAAALTKSEHTLVVLIVS------------GGAGPEAGA 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 331 VWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTF-KYAFDYKSTDVYWCTADCGWITGH 409
Cdd:cd05959  142 LLLAELVAAEAEQLKPAATHADDPAFWLYSSGSTGRPKGVVHLHAD-IYWTAELYaRNVLGIREDDVCFSAAKLFFAYGL 220
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 410 SYVTYGPMLNGATVVVFegaPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPINPS 489
Cdd:cd05959  221 GNSLTFPLSVGATTVLM---PERPTPAAVFKRIRRYRPTVFFGVPTLYAAMLAAPN--LPSRDLSSLRLCVSAGEALPAE 295
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 490 AWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpga 569
Cdd:cd05959  296 VGERWKARFG---LDILDGIGSTEMLHIFLSNRPGR--VRYGTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGP---- 366
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 570 fRTLFGDHERYETTYfKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEH 648
Cdd:cd05959  367 -SSATMYWNNRDKTR-DTFQGEWTrTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVED 444
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 649 EVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05959  445 EDGLTKPKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
181-712 1.24e-65

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 226.99  E-value: 1.24e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWeGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd05970   32 DKLALVW-CDDAGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPATHQLTAKD 110
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILtCNAVKRGPKTINlKAIVDAALDQ---SSKDGVSVGIClTYDNSLATTREntkwqngrdvwwqdvI 337
Cdd:cd05970  111 IVYRIESADIKMIV-AIAEDNIPEEIE-KAAPECPSKPklvWVGDPVPEGWI-DFRKLIKNASP---------------D 172
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 338 SQYPTSCEveWVDAEDPLFLLYTSGSTGKPKGVLHTTGgYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPM 417
Cdd:cd05970  173 FERPTANS--YPCGEDILLVYFSSGTTGMPKMVEHDFT-YPLGHIVTAKYWQNVREGGLHLTVADTGWGKAVWGKIYGQW 249
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVVFEGapNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRHSRKSLRVLGSVGEPINPSAWRWFFNV 497
Cdd:cd05970  250 IAGAAVFVYDY--DKFDPKALLEKLSKYGVTTFCAPPTIYRFLIRED---LSRYDLSSLRYCTTAGEALNPEVFNTFKEK 324
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 VGDSrcpISDTWWQTETGgFMITPLPGAWPqKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCV---KGSWPGAFRTLF 574
Cdd:cd05970  325 TGIK---LMEGFGQTETT-LTIATFPWMEP-KPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIrtsKGKPVGLFGGYY 399
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 GDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQG 654
Cdd:cd05970  400 KDAEKTAEVWHD---GYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQV 476
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 655 IYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05970  477 VKATIVLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIR 534
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
171-712 6.84e-63

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 217.82  E-value: 6.84e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVeAGLGDKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhs 250
Cdd:cd05936    5 LEEAA-RRFPDKTALIFMG------RKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAV-- 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 251 VVfagfsadslaqrivdckP-NVILTcnavkrgpkTINLKAIVDaaldqssKDGVSVGICltydnslattrentkwqngr 329
Cdd:cd05936   76 VV-----------------PlNPLYT---------PRELEHILN-------DSGAKALIV-------------------- 102
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 330 DVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGG-YMIYTATTFKYAFDYKSTDVYWCTADCgwitg 408
Cdd:cd05936  103 AVSFTDLLAAGAPLGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRNlVANALQIKAWLEDLLEGDDVVLAALPL----- 177
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 409 hsYVTYG-------PMLNGATVVVFegaPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGS 481
Cdd:cd05936  178 --FHVFGltvalllPLALGATIVLI---PR-FRPIGVLKEIRKHRVTIFPGVPTMYIALLNAPE--FKKRDFSSLRLCIS 249
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 482 VGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGgfmitPL----PGAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GEc 556
Cdd:cd05936  250 GGAPLPVEVAERFEELTG---VPIVEGYGLTETS-----PVvavnPLDGPRKPGSIGIPLPGTEVKIVDDDGEELPpGE- 320
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGswPGAFRTLFGDHERYETTyFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:cd05936  321 VGELWVRG--PQVMKGYWNRPEETAEA-FV--DGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHP 395
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05936  396 AVAEAAVVGVPDPYSGEAVKAFVVLKEGASLTEE---EIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
168-712 9.76e-63

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 218.52  E-value: 9.76e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 168 YNCLDKNVEAgLGDKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKDNGVKKGDAVVIYL---PMLMElpiAMLACAR 244
Cdd:PRK06187   9 GRILRHGARK-HPDKEAVYFDGRRT------TYAELDERVNRLANALRALGVKKGDRVAVFDwnsHEYLE---AYFAVPK 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 245 IGAV-HSV-VFagFSADSLAQRIVDCKPNVILTcnavkrGPKTINLKAIVDAALDqsskdgvSVGICLTYDnslATTREN 322
Cdd:PRK06187  79 IGAVlHPInIR--LKPEEIAYILNDAEDRVVLV------DSEFVPLLAAILPQLP-------TVRTVIVEG---DGPAAP 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 323 TKWQNGRdvwWQDVIS-QYPTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHT----TGGYMIYTAttfkyAFDYKSTDVY 397
Cdd:PRK06187 141 LAPEVGE---YEELLAaASDTFDFPD-IDENDAAAMLYTSGTTGHPKGVVLShrnlFLHSLAVCA-----WLKLSRDDVY 211
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 398 -----------WctadcGWitghsyvTYGPMLNGATVVvfegapnYP---DPGRCWDIVDKYKVSIFYTAPTLVRSLMRD 463
Cdd:PRK06187 212 lvivpmfhvhaW-----GL-------PYLALMAGAKQV-------IPrrfDPENLLDLIETERVTFFFAVPTIWQMLLKA 272
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 464 ddKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETGGFM-ITPL----PGAWPqKPGSATFPFF 538
Cdd:PRK06187 273 --PRAYFVDFSSLRLVIYGGAALPPALLREFKEKFG---IDLVQGYGMTETSPVVsVLPPedqlPGQWT-KRRSAGRPLP 346
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 539 GVQPVIVDEKGNEIE--GECSGYLCVKGSWpgafRTL--FGDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDD 614
Cdd:PRK06187 347 GVEARIVDDDGDELPpdGGEVGEIIVRGPW----LMQgyWNRPEATAETIDG---GWLHTGDVGYIDEDGYLYITDRIKD 419
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 615 VINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSE-ELRKSLvlmvRNQIGAFAAPDRIH 693
Cdd:PRK06187 420 VIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDAkELRAFL----RGRLAKFKLPKRIA 495
                        570
                 ....*....|....*....
gi 378548265 694 WAPGLPKTRSGKIMRRILR 712
Cdd:PRK06187 496 FVDELPRTSVGKILKRVLR 514
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
180-708 2.06e-62

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 215.55  E-value: 2.06e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:cd17631    9 PDRTALVFGGR------SLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPP 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILtcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisq 339
Cdd:cd17631   83 EVAYILADSGAKVLF----------------------------------------------------------------- 97
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 yptscevewvdaEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFkYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLN 419
Cdd:cd17631   98 ------------DDLALLMYTSGTTGRPKGAMLTHRNLLWNAVNAL-AALDLGPDDVLLVVAPLFHIGGLGVFTLPTLLR 164
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVFEGapnyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPINPSAWR--WFFNV 497
Cdd:cd17631  165 GGTVVILRK----FDPETVLDLIERHRVTSFFLVPTMIQALLQHPRFATTDLS--SLRAVIYGGAPMPERLLRalQARGV 238
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 VgdsrcpISDTWWQTETGGfMITPLPGAWPQ-KPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGD 576
Cdd:cd17631  239 K------FVQGYGMTETSP-GVTFLSPEDHRrKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRG--PHVMAGYWNR 309
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 577 HERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIY 656
Cdd:cd17631  310 PEATAAAFRD---GWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVV 386
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|..
gi 378548265 657 AFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17631  387 AVVVPRPGAELDED---ELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
195-712 2.20e-61

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 212.71  E-value: 2.20e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd05919    8 DRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVV 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDP 354
Cdd:cd05919   88 T--------------------------------------------------------------------------SADDI 93
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADC--GWITGHSyvTYGPMLNGATVVVFEGAPny 432
Cdd:cd05919   94 AYLLYSSGTTGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMffGYGLGNS--LWFPLAVGASAVLNPGWP-- 169
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 433 pDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQT 512
Cdd:cd05919  170 -TAERVLATLARFRPTVLYGVPTFYANLLDSCA--GSPDALRSLRLCVSAGEALPRGLGERWMEHFG---GPILDGIGAT 243
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 513 ETGGFMITPLPGAWpqKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETTYFkpfAGYY 592
Cdd:cd05919  244 EVGHIFLSNRPGAW--RLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRG--PSAAVGYWNNPEKSRATFN---GGWY 316
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELR 672
Cdd:cd05919  317 RTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLA 396
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 378548265 673 KSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05919  397 RDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
PRK08316 PRK08316
acyl-CoA synthetase; Validated
181-713 1.12e-60

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 213.26  E-value: 1.12e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK08316  26 DKTALVFG------DRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEE 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAvkrgpktinLKAIVDAALDQSSKDGVsvgiclTYDNSLATTRENTKWQNGRDvwWQDviSQY 340
Cdd:PRK08316 100 LAYILDHSGARAFLVDPA---------LAPTAEAALALLPVDTL------ILSLVLGGREAPGGWLDFAD--WAE--AGS 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMI-YTATTFkyAFDYKSTDV-------YWCTAdcgwitghSYV 412
Cdd:PRK08316 161 VAEPDVE-LADDDLAQILYTSGTESLPKGAMLTHRALIAeYVSCIV--AGDMSADDIplhalplYHCAQ--------LDV 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 413 TYGPMLN-GATVVVFEGapnyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkFVTRHSRkSLR------------VL 479
Cdd:PRK08316 230 FLGPYLYvGATNVILDA----PDPELILRTIEAERITSFFAPPTVWISLLRHPD-FDTRDLS-SLRkgyygasimpveVL 303
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 GSVGEPInPSAWrwFFNVVGdsrcpisdtwwQTEtggfmITPL-----PGAWPQKPGSATFPFFGVQPVIVDEKGNEI-E 553
Cdd:PRK08316 304 KELRERL-PGLR--FYNCYG-----------QTE-----IAPLatvlgPEEHLRRPGSAGRPVLNVETRVVDDDGNDVaP 364
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 554 GE-----------CSGYlcvkgswpgafrtlFGDHERYETTyfkpFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGH 621
Cdd:PRK08316 365 GEvgeivhrspqlMLGY--------------WDDPEKTAEA----FRGGWFhSGDLGVMDEEGYITVVDRKKDMIKTGGE 426
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 622 RIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKT 701
Cdd:PRK08316 427 NVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTED---ELIAHCRARLAGFKVPKRVIFVDELPRN 503
                        570
                 ....*....|..
gi 378548265 702 RSGKIMRRILRK 713
Cdd:PRK08316 504 PSGKILKRELRE 515
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
187-713 3.07e-58

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 206.55  E-value: 3.07e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 187 WEGNELGVDASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRI 265
Cdd:cd05928   31 WWVNGKGDEVKWSFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRL 110
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 266 VDCKPNVILTCNAvkrgpktinLKAIVDAALDQsskdgvsvgiCLTYDNSLATTrentkwQNGRDVW--WQDVISQYPTS 343
Cdd:cd05928  111 QASKAKCIVTSDE---------LAPEVDSVASE----------CPSLKTKLLVS------EKSRDGWlnFKELLNEASTE 165
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 344 CEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATV 423
Cdd:cd05928  166 HHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQGACV 245
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEgAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRHSRKSLRVLGSVGEPINPSA---WRwffNVVGd 500
Cdd:cd05928  246 FVHH-LPRF-DPLVILKTLSSYPITTFCGAPTVYRMLVQQD---LSSYKFPSLQHCVTGGEPLNPEVlekWK---AQTG- 316
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 501 srCPISDTWWQTETGgfMITPLPGAWPQKPGS--ATFPFFGVQpvIVDEKGNEI----EGECSgyLCVKGSWP-GAFRTL 573
Cdd:cd05928  317 --LDIYEGYGQTETG--LICANFKGMKIKPGSmgKASPPYDVQ--IIDDNGNVLppgtEGDIG--IRVKPIRPfGLFSGY 388
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FGDHERYETTYFKPFagyYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:cd05928  389 VDNPEKTAATIRGDF---YLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGE 465
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 654 GIYAFVTLleGVPYS----EELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05928  466 VVKAFVVL--APQFLshdpEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
194-707 1.11e-53

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 192.81  E-value: 1.11e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 194 VDA----SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:cd05911    3 IDAdtgkELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISK 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAVkrgpktinlkaiVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQ--NGRDVWWQDVISQyptsceve 347
Cdd:cd05911   83 PKVIFTDPDG------------LEKVKEAAKELGPKDKIIVLDDKPDGVLSIEDLLSptLGEEDEDLPPPLK-------- 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 wVDAEDPLFLLYTSGSTGKPKGVL--HTTGGYMIYTATTFKYAfDYKSTDVYWCTADCGWITG-HSYVTYgpMLNGATVV 424
Cdd:cd05911  143 -DGKDDTAAILYSSGTTGLPKGVClsHRNLIANLSQVQTFLYG-NDGSNDVILGFLPLYHIYGlFTTLAS--LLNGATVI 218
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 425 VFegapNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPInpsaWRWFFNVVG--DSR 502
Cdd:cd05911  219 IM----PKFDSELFLDLIEKYKITFLYLVPPIAAALAKSPL--LDKYDLSSLRVILSGGAPL----SKELQELLAkrFPN 288
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 503 CPISDTWWQTETGGfMITPLPGaWPQKPGSATFPFFGVQPVIVDEKGNEIEGECS-GYLCVKGswPGAFRTLFGDHERYE 581
Cdd:cd05911  289 ATIKQGYGMTETGG-ILTVNPD-GDDKPGSVGRLLPNVEAKIVDDDGKDSLGPNEpGEICVRG--PQVMKGYYNNPEATK 364
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 582 TTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTL 661
Cdd:cd05911  365 ETFDE--DGWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVR 442
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 378548265 662 LEGvpySEELRKSLVLMVRNQIGAFAA-PDRIHWAPGLPKTRSGKIM 707
Cdd:cd05911  443 KPG---EKLTEKEVKDYVAKKVASYKQlRGGVVFVDEIPKSASGKIL 486
benz_CoA_lig TIGR02262
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ...
171-713 4.96e-52

benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.


Pssm-ID: 274059 [Multi-domain]  Cd Length: 505  Bit Score: 188.89  E-value: 4.96e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  171 LDKNVEAGLGDKTAIhwegnelgVD--ASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV 248
Cdd:TIGR02262  10 LDRNVVEGRGGKTAF--------IDdiSSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIV 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  249 HSVVFAGFSADSLAQRIVDCKPNVILTCN--------AVKRGPktiNLKAIVDAALDQSSKDGVSvgicltydNSLATTr 320
Cdd:TIGR02262  82 PVALNTLLTADDYAYMLEDSRARVVFVSGallpvikaALGKSP---HLEHRVVVGRPEAGEVQLA--------ELLATE- 149
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  321 entkwqngrdvwwQDVISQYPTScevewvdAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTF-KYAFDYKSTDVYWC 399
Cdd:TIGR02262 150 -------------SEQFKPAATQ-------ADDPAFWLYSSGSTGMPKGVVHTHSN-PYWTAELYaRNTLGIREDDVCFS 208
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  400 TADCGWITGHSYVTYGPMLNGATVVVFegaPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVL 479
Cdd:TIGR02262 209 AAKLFFAYGLGNALTFPMSVGATTVLM---GERPTPDAVFDRLRRHQPTIFYGVPTLYAAMLADPN--LPSEDQVRLRLC 283
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  480 GSVGEPINPSA---WRWFFNVvgdsrcPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:TIGR02262 284 TSAGEALPAEVgqrWQARFGV------DIVDGIGSTEMLHIFLSNLPGD--VRYGTSGKPVPGYRLRLVGDGGQDVADGE 355
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  557 SGYLCVKGswPGAFRTLFGDHERYETTYFKPFAGyyfSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:TIGR02262 356 PGELLISG--PSSATMYWNNRAKSRDTFQGEWTR---SGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHP 430
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265  637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGvpySEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:TIGR02262 431 AVLEAAVVGVADEDGLIKPKAFVVLRPG---QTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
195-713 2.03e-51

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 186.75  E-value: 2.03e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd05926   12 TPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVL 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCN-----AVKRGPKTinLKAIVDAALDqsskdgvsVGICLTYD--NSLATTRENTKWQNGRDVwwqdvisqyptsceve 347
Cdd:cd05926   92 TPKgelgpASRAASKL--GLAILELALD--------VGVLIRAPsaESLSNLLADKKNAKSEGV---------------- 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 wVDAEDPLFLLYTSGSTGKPKGVLHTTGgyMIYTATTfKYAFDYKSTDvywctADCGWIT-----GHSYVT--YGPMLNG 420
Cdd:cd05926  146 -PLPDDLALILHTSGTTGRPKGVPLTHR--NLAASAT-NITNTYKLTP-----DDRTLVVmplfhVHGLVAslLSTLAAG 216
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVfegaPNYPDPGRCWDIVDKYKVSiFYTA-PTLVRSLMRDDDKfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVG 499
Cdd:cd05926  217 GSVVL----PPRFSASTFWPDVRDYNAT-WYTAvPTIHQILLNRPEP-NPESPPPKLRFIRSCSASLPPAVLEALEATFG 290
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dsrCPISDTWWQTETGGFMIT-PLPGAwPQKPGSATFPFfGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHE 578
Cdd:cd05926  291 ---APVLEAYGMTEAAHQMTSnPLPPG-PRKPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRG--PNVTRGYLNNPE 363
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 579 RYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAF 658
Cdd:cd05926  364 ANAEAAFK--DGWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAA 441
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 659 VTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05926  442 VVLREGASVTeEELRAFC----RKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
199-712 4.51e-51

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 184.03  E-value: 4.51e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcna 278
Cdd:cd05934    5 TYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV--- 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 279 vkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaeDPLFLL 358
Cdd:cd05934   82 --------------------------------------------------------------------------DPASIL 87
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 359 YTSGSTGKPKGVLhTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVfegAPNYpDPGRC 438
Cdd:cd05934   88 YTSGTTGPPKGVV-ITHANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVL---LPRF-SASRF 162
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 439 WDIVDKYKVSIFYTAPTLVRSLM----RDDDkfvtRHSRksLRVLGsvGEPINPSAWRWFFNVVGdsrCPISDTWWQTET 514
Cdd:cd05934  163 WSDVRRYGATVTNYLGAMLSYLLaqppSPDD----RAHR--LRAAY--GAPNPPELHEEFEERFG---VRLLEGYGMTET 231
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGFMITPLPGawPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWP-GAFRTLFGDHEryETTyfKPFA-GYY 592
Cdd:cd05934  232 IVGVIGPRDE--PRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRGLRGwGFFKGYYNMPE--ATA--EAMRnGWF 305
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElr 672
Cdd:cd05934  306 HTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPE-- 383
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 378548265 673 kSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05934  384 -ELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
198-715 5.59e-51

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 183.92  E-value: 5.59e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRivdckpnviltcn 277
Cdd:cd05974    1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDR------------- 67
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 aVKRGPKTInlkAIVDaaldqsskdgvsvgicltydnslattrENTKwqngrdvwwqdvisqyptscevewvdAEDPLFL 357
Cdd:cd05974   68 -VDRGGAVY---AAVD---------------------------ENTH--------------------------ADDPMLL 90
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGYMIYTATTFkYAFDYKSTDVYWCTADCGWiTGHSYVT-YGPMLNGATVVVFegapNYP--D 434
Cdd:cd05974   91 YFTSGTTSKPKLVEHTHRSYPVGHLSTM-YWIGLKPGDVHWNISSPGW-AKHAWSCfFAPWNAGATVFLF----NYArfD 164
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 435 PGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRhSRKSLRVLGSVGEPINPSAWRWFFNVVGDSrcpISDTWWQTET 514
Cdd:cd05974  165 AKRVLAALVRYGVTTLCAPPTVWRMLIQQD---LAS-FDVKLREVVGAGEPLNPEVIEQVRRAWGLT---IRDGYGQTET 237
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGfMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNEI-EGECSgyLCVKGSWP-GAFRTLFGDHERyetTYFKPFAGYY 592
Cdd:cd05974  238 TA-LVGNSPGQ-PVKAGSMGRPLPGYRVALLDPDGAPAtEGEVA--LDLGDTRPvGLMKGYAGDPDK---TAHAMRGGYY 310
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELR 672
Cdd:cd05974  311 RTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPETA 390
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|...
gi 378548265 673 KSLVLMVRNQIGAFAAPDRIHWAPgLPKTRSGKIMRRILRKIA 715
Cdd:cd05974  391 LEIFRFSRERLAPYKRIRRLEFAE-LPKTISGKIRRVELRRRE 432
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
195-712 5.53e-49

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 178.44  E-value: 5.53e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGV-KKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVI 273
Cdd:cd05958    8 EREWTYRDLLALANRIANVLVGELGiVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKARITVA 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 274 LTCNAVkrgpktinlkaivdaaldqsskdgvsvgicltydnslaTTREntkwqngrdvwwqdvisqyptscevewvdaeD 353
Cdd:cd05958   88 LCAHAL--------------------------------------TASD-------------------------------D 98
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGApnyp 433
Cdd:cd05958   99 ICILAFTSGTTGAPKATMHFHRDPLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEA---- 174
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 434 DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTE 513
Cdd:cd05958  175 TPDLLLSAIARYKPTVLFTAPTAYRAMLAHPDA--AGPDLSSLRKCVSAGEALPAALHRAWKEATG---IPIIDGIGSTE 249
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 514 TGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERyetTYFKpfAGYYF 593
Cdd:cd05958  250 MFHIFISARPGD--ARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRG--PTGCRYLADKRQR---TYVQ--GGWNI 320
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 594 SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRK 673
Cdd:cd05958  321 TGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLAR 400
                        490       500       510
                 ....*....|....*....|....*....|....*....
gi 378548265 674 SLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05958  401 ELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
181-709 2.17e-48

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 176.95  E-value: 2.17e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd05930    2 DAVAVVDGDQ------SLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAER 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd05930   76 LAYILEDSGAKLVLT----------------------------------------------------------------- 90
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATtFKYAFDYKSTDVYWCTADCGWItGHSYVTYGPMLNG 420
Cdd:cd05930   91 ---------DPDDLAYVIYTSGSTGKPKGVMVEHRGLVNLLLW-MQEAYPLTPGDRVLQFTSFSFD-VSVWEIFGALLAG 159
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVfegAPN--YPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVtrhsRKSLRVLGSVGEPINPSAWR-WF--- 494
Cdd:cd05930  160 ATLVV---LPEevRKDPEALADLLAEEGITVLHLTPSLLRLLLQELELAA----LPSLRLVLVGGEALPPDLVRrWRell 232
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 -----FNVVGDSRCPISDTWWQTETGGFMITPLP-GAwpqkpgsatfPFFGVQPVIVDEKGNE----IEGEcsgyLCVKG 564
Cdd:cd05930  233 pgarlVNLYGPTEATVDATYYRVPPDDEEDGRVPiGR----------PIPNTRVYVLDENLRPvppgVPGE----LYIGG 298
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 565 swPGAFRtlfGDHERYETTYFK----PFAG---YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 637
Cdd:cd05930  299 --AGLAR---GYLNRPELTAERfvpnPFGPgerMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLAHPG 373
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 638 CAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRR 709
Cdd:cd05930  374 VREAAVVAREDGDGEKRLVAYVVPDEGGELDeEELRAHL----AERLPDYMVPSAFVVLDALPLTPNGKVDRK 442
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
197-713 6.80e-47

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 172.57  E-value: 6.80e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTc 276
Cdd:cd05903    1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVV- 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngRDVWWQDVISQYPtscevewvdaEDPLF 356
Cdd:cd05903   80 ----------------------------------------------------PERFRQFDPAAMP----------DAVAL 97
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYMiytATTFKYAFDYKST--DVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGApnypD 434
Cdd:cd05903   98 LLFTSGTTGEPKGVMHSHNTLS---ASIRQYAERLGLGpgDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIW----D 170
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 435 PGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRksLRVLGSVGEPINPSAWRWFFNVVGDSRCPIsdtWWQTET 514
Cdd:cd05903  171 PDKALALMREHGVTFMMGATPFLTDLLNAVEEAGEPLSR--LRTFVCGGATVPRSLARRAAELLGAKVCSA---YGSTEC 245
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFrtlFGDHERYETTYFKPFAGYYFS 594
Cdd:cd05903  246 PGAVTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRG--PSVF---LGYLDRPDLTADAAPEGWFRT 320
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 595 GDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEG-VPYSEELRK 673
Cdd:cd05903  321 GDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGaLLTFDELVA 400
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|
gi 378548265 674 SLvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05903  401 YL---DRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRLRE 437
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
179-713 4.14e-43

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 163.54  E-value: 4.14e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 179 LGDKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSA 258
Cdd:PRK07656  18 FGDKEAYVFGDQ------RLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYTA 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 DSLAQRIVDCKPNVILTCNAVkrgpktinlkaivdAALDQSSKDGV----SVGICLTYDNSLATTRENTkwqngrdvwWQ 334
Cdd:PRK07656  92 DEAAYILARGDAKALFVLGLF--------------LGVDYSATTRLpaleHVVICETEEDDPHTEKMKT---------FT 148
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 335 DVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLhTTGGYMIYTATTFKYAFDYKSTDVYWC----------TAdcG 404
Cdd:PRK07656 149 DFLAAGDPAERAPEVDPDDVADILFTSGTTGRPKGAM-LTHRQLLSNAADWAEYLGLTEGDRYLAanpffhvfgyKA--G 225
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 405 WITghsyvtygPMLNGATVV---VFegapnypDPGRCWDIVDKYKVSIFYTAPTLVRSL----MRDDDKFvtrhsrKSLR 477
Cdd:PRK07656 226 VNA--------PLMRGATILplpVF-------DPDEVFRLIETERITVLPGPPTMYNSLlqhpDRSAEDL------SSLR 284
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 478 VLGSVGEPINPSAWRWF---FNVVgdsrcPISDTWWQTETGGFM-ITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIE 553
Cdd:PRK07656 285 LAVTGAASMPVALLERFeseLGVD-----IVLTGYGLSEASGVTtFNRLDDDRKTVAGTIGTAIAGVENKIVNELGEEVP 359
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 554 GECSGYLCVKGswPGAFRTLFGDHEryETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV 633
Cdd:PRK07656 360 VGEVGELLVRG--PNVMKGYYDDPE--ATAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLY 435
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 634 LHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK07656 436 EHPAVAEAAVIGVPDERLGEVGKAYVVLKPGAELTEE---ELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
180-715 1.13e-42

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 162.62  E-value: 1.13e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIhwegneLGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV-------H--- 249
Cdd:COG1021   39 PDRIAV------VDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIpvfalpaHrra 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 250 ----------------SVVFAGFSADSLAQRIVDCKPnviltcnavkrGPKTInlkaIVDAaldqSSKDGVSvgicltyd 313
Cdd:COG1021  113 eishfaeqseavayiiPDRHRGFDYRALARELQAEVP-----------SLRHV----LVVG----DAGEFTS-------- 165
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 314 nslattrentkwqngrdvwWQDVISQyPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYmIYTATTFKYAFDYKS 393
Cdd:COG1021  166 -------------------LDALLAA-PADLSEPRPDPDDVAFFQLSGGTTGLPKLIPRTHDDY-LYSVRASAEICGLDA 224
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 394 TDVYWCTADcgwiTGHSY-----VTYGPMLNGATVVVfegAPNyPDPGRCWDIVDKYKVSIfyTA--PTLVRSLMRDDDK 466
Cdd:COG1021  225 DTVYLAALP----AAHNFplsspGVLGVLYAGGTVVL---APD-PSPDTAFPLIERERVTV--TAlvPPLALLWLDAAER 294
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 467 fvTRHSRKSLRVLGSVGEPINPSAWR--------WFFNVVG------------DSR--------CPIS--DTWWqtetgg 516
Cdd:COG1021  295 --SRYDLSSLRVLQVGGAKLSPELARrvrpalgcTLQQVFGmaeglvnytrldDPEevilttqgRPISpdDEVR------ 366
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 517 fmitplpgawpqkpgsatfpffgvqpvIVDEKGNEI-EGECsGYLCVKGswPGAFRTLFGDHErYETTYFKPfAGYYFSG 595
Cdd:COG1021  367 ---------------------------IVDEDGNPVpPGEV-GELLTRG--PYTIRGYYRAPE-HNARAFTP-DGFYRTG 414
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 596 DGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSL 675
Cdd:COG1021  415 DLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPRGEPLTLAELRRFL 494
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|.
gi 378548265 676 vlmvRNQ-IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:COG1021  495 ----RERgLAAFKLPDRLEFVDALPLTAVGKIDKKALRAAL 531
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
199-643 6.25e-42

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 157.81  E-value: 6.25e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  199 TYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcn 277
Cdd:TIGR01733   1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLT-- 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  278 avkrgpktinlkaivDAALDQSSKDGVSVGICLTYDNSLATTRENTKwqngrdvwwqdvisqyPTSCEVewVDAEDPLFL 357
Cdd:TIGR01733  79 ---------------DSALASRLAGLVLPVILLDPLELAALDDAPAP----------------PPPDAP--SGPDDLAYV 125
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  358 LYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVywctadcgWITGHSYV-------TYGPMLNGATVVVFEGAP 430
Cdd:TIGR01733 126 IYTSGSTGRPKGVVVTHRS-LVNLLAWLARRYGLDPDDR--------VLQFASLSfdasveeIFGALLAGATLVVPPEDE 196
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  431 NYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhSRKSLRVLGSVGEPINPSA---WRWffnVVGDSRcpisd 507
Cdd:TIGR01733 197 ERDDAALLAALIAEHPVTVLNLTPSLLALLAAALPP-----ALASLRLVILGGEALTPALvdrWRA---RGPGAR----- 263
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  508 tWWQ----TE-TGGFMITPLPGAWPQKPGSATF--PFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERY 580
Cdd:TIGR01733 264 -LINlygpTEtTVWSTATLVDPDDAPRESPVPIgrPLANTRLYVLDDDLRPVPVGVVGELYIGG--PGVARGYLNRPELT 340
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 378548265  581 ETTYFK-PFAG-----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAV 643
Cdd:TIGR01733 341 AERFVPdPFAGgdgarLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
180-711 1.22e-41

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 158.61  E-value: 1.22e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:cd12116    1 PDATAVRDD------DRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPAD 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTcnavkrgpktinlkaivdaalDQSSKDGVSVGICLTYDNSLATTRentkwqngrdvwwqDVISQ 339
Cdd:cd12116   75 RLRYILEDAEPALVLT---------------------DDALPDRLPAGLPVLLLALAAAAA--------------APAAP 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 YPTscevewVDAEDPLFLLYTSGSTGKPKGV-----------------LHTTGGYMIYTATTfkYAFDYKSTDVYWctad 402
Cdd:cd12116  120 RTP------VSPDDLAYVIYTSGSTGRPKGVvvshrnlvnflhsmrerLGLGPGDRLLAVTT--YAFDISLLELLL---- 187
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 403 cgwitghsyvtygPMLNGATVVVFEGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhSRKSLRVL-Gs 481
Cdd:cd12116  188 -------------PLLAGARVVIAPRETQR-DPEALARLIEAHSITVMQATPATWRMLLDAGWQ-----GRAGLTALcG- 247
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 482 vGEPINP-----------SAWrwffNVVGdsrcPISDTWWQTetggfmITPLPGAWPQKPgsATFPFFGVQPVIVDEKGN 550
Cdd:cd12116  248 -GEALPPdlaarllsrvgSLW----NLYG----PTETTIWST------AARVTAAAGPIP--IGRPLANTQVYVLDAALR 310
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 551 EI-EGECsGYLCVKGswPGAFRtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGH 621
Cdd:cd12116  311 PVpPGVP-GELYIGG--DGVAQ---GYLGRPALTAERfvpdPFAGpgsrLYRTGDLVRRRADGRLEYLGRADGQVKIRGH 384
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 622 RIGTAEVESALVLHPQCAEAAVVgIEHEVKGQGIYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPK 700
Cdd:cd12116  385 RIELGEIEAALAAHPGVAQAAVV-VREDGGDRRLVAYVVLKAGAAPDaAALRAHL----RATLPAYMVPSAFVRLDALPL 459
                        570
                 ....*....|.
gi 378548265 701 TRSGKIMRRIL 711
Cdd:cd12116  460 TANGKLDRKAL 470
PLN03052 PLN03052
acetate--CoA ligase; Provisional
96-728 5.04e-41

acetate--CoA ligase; Provisional


Pssm-ID: 215553 [Multi-domain]  Cd Length: 728  Bit Score: 160.63  E-value: 5.04e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  96 KEFSGQALVSSPQQYMEMHKRSMDDPaafwsdiasEFYWKQKWGDQ--VFSENldvrkgPISI-----------EWFKGG 162
Cdd:PLN03052 108 KELLGSKYKDPISSFSEFQRFSVENP---------EVYWSIVLDELslVFSVP------PRCIldtsdesnpggQWLPGA 172
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 163 ITNICYNCLDKNVEAGLgDKTAIHW--EGNE-LGVDaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAM 239
Cdd:PLN03052 173 VLNVAECCLTPKPSKTD-DSIAIIWrdEGSDdLPVN-RMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIY 250
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 240 LACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKA-IVDAaldQSSK------DGVSVGICLty 312
Cdd:PLN03052 251 LAIILAGCVVVSIADSFAPSEIATRLKISKAKAIFTQDVIVRGGKSIPLYSrVVEA---KAPKaivlpaDGKSVRVKL-- 325
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 313 dnslattRENtkwqngrDVWWQDVISQY-PTSCEVEWVDAEDPL----FLLYTSGSTGKPKGVLHTtggymiyTATTFKY 387
Cdd:PLN03052 326 -------REG-------DMSWDDFLARAnGLRRPDEYKAVEQPVeaftNILFSSGTTGEPKAIPWT-------QLTPLRA 384
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 388 A------FDYKSTDVY-WCTaDCGWITGHsYVTYGPMLNGATVVVFEGAPNYPDPGRcwdIVDKYKVSIFYTAPTLVRS- 459
Cdd:PLN03052 385 AadawahLDIRKGDIVcWPT-NLGWMMGP-WLVYASLLNGATLALYNGSPLGRGFAK---FVQDAKVTMLGTVPSIVKTw 459
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 460 ----LMRDDDKFvtrhsrkSLRVLGSVGEPINPSAWRWFFnvvgdSRC---PISDTWWQTETGGFMITplpGAWPQKPGS 532
Cdd:PLN03052 460 kntnCMAGLDWS-------SIRCFGSTGEASSVDDYLWLM-----SRAgykPIIEYCGGTELGGGFVT---GSLLQPQAF 524
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 533 ATF--PFFGVQPVIVDEKGNEI--EGECSGYLCVKGSWPGAFRTLF-GDHERyetTYFK--P-FAGYYFS--GDGCSRDK 602
Cdd:PLN03052 525 AAFstPAMGCKLFILDDSGNPYpdDAPCTGELALFPLMFGASSTLLnADHYK---VYFKgmPvFNGKILRrhGDIFERTS 601
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 603 DGYYWLTGRVDDVINVSGHRIGTAEVESAL-VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS----EELRKSLVL 677
Cdd:PLN03052 602 GGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLETAAIGVPPPGGGPEQLVIAAVLKDPPGSnpdlNELKKIFNS 681
                        650       660       670       680       690
                 ....*....|....*....|....*....|....*....|....*....|.
gi 378548265 678 MVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKiasrQLEELGDTSTL 728
Cdd:PLN03052 682 AIQKKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQ----QLAQELSRSKL 728
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
194-711 7.90e-41

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 157.01  E-value: 7.90e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 194 VDAS----LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCK 269
Cdd:cd05904   25 IDAAtgraLTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSG 104
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 270 PNVILTCNAvkrgpktiNLKAIVDAALdqsskdgvSVGICLTYDNSLATTRENTKWQNgrdvwwqdvisqyPTSCEVEWV 349
Cdd:cd05904  105 AKLAFTTAE--------LAEKLASLAL--------PVVLLDSAEFDSLSFSDLLFEAD-------------EAEPPVVVI 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTA-TTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEG 428
Cdd:cd05904  156 KQDDVAALLYSSGTTGRSKGVMLTHRNLIAMVAqFVAGEGSNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVVMPR 235
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 429 ApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDsrCPISDT 508
Cdd:cd05904  236 F----DLEELLAAIERYKVTHLPVVPPIVLALVKSPI--VDKYDLSSLRQIMSGAAPLGKELIEAFRAKFPN--VDLGQG 307
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 509 WWQTE-TGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGEC-SGYLCVKGswPGAFRTLFGDHERYETTYFK 586
Cdd:cd05904  308 YGMTEsTGVVAMCFAPEKDRAKYGSVGRLVPNVEAKIVDPETGESLPPNqTGELWIRG--PSIMKGYLNNPEATAATIDK 385
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 587 pfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVP 666
Cdd:cd05904  386 --EGWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKPGSS 463
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*
gi 378548265 667 YSEELRKSLvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05904  464 LTEDEIMDF---VAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
PRK07529 PRK07529
AMP-binding domain protein; Validated
197-735 1.51e-39

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 155.11  E-value: 1.51e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLAcariGAVHSVVFA---GFSADSLAQRIVDCKPNVI 273
Cdd:PRK07529  58 TWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWG----GEAAGIANPinpLLEPEQIAELLRAAGAKVL 133
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 274 LT-------------CNAVKRGPktiNLKAIVDAALdqsskdgvsvGICLTYDNSLATTRENTKWQNgRDVWWQDVISQY 340
Cdd:PRK07529 134 VTlgpfpgtdiwqkvAEVLAALP---ELRTVVEVDL----------ARYLPGPKRLAVPLIRRKAHA-RILDFDAELARQ 199
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PTSCEV--EWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGhSYVT-YGPM 417
Cdd:PRK07529 200 PGDRLFsgRPIGPDDVAAYFHTGGTTGMPKLAQHTHGN-EVANAWLGALLLGLGPGDTVFCGLPLFHVNA-LLVTgLAPL 277
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVvFEGAPNYPDPG---RCWDIVDKYKVSIFYTAPTLVRSLMrddDKFVTRHSRKSLRVLGSVGEPINPSAWRWF 494
Cdd:PRK07529 278 ARGAHVV-LATPQGYRGPGviaNFWKIVERYRINFLSGVPTVYAALL---QVPVDGHDISSLRYALCGAAPLPVEVFRRF 353
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 FNVVGdsrCPISDTWWQTE-TGGFMITPLPGawPQKPGSA--TFPFFGVQPVIVDEKGNEIEgECS----GYLCVKGswP 567
Cdd:PRK07529 354 EAATG---VRIVEGYGLTEaTCVSSVNPPDG--ERRIGSVglRLPYQRVRVVILDDAGRYLR-DCAvdevGVLCIAG--P 425
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 GAFRT-LFGDHER---YETTYFKpfagyyfSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAV 643
Cdd:PRK07529 426 NVFSGyLEAAHNKglwLEDGWLN-------TGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLRHPAVALAAA 498
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 644 VGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAA-PDRIHWAPGLPKTRSGKIMRRILRKIASRQ---- 718
Cdd:PRK07529 499 VGRPDAHAGELPVAYVQLKPGASATEA---ELLAFARDHIAERAAvPKHVRILDALPKTAVGKIFKPALRRDAIRRvlra 575
                        570
                 ....*....|....*...
gi 378548265 719 -LEELGdtstlADPSVVD 735
Cdd:PRK07529 576 aLRDAG-----VEAEVVD 588
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
181-712 2.37e-39

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 152.11  E-value: 2.37e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd17651   10 DAPALVAEG------RRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAER 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdAALDQSSKDGVSVGICLTYDNSLATtrentkwqngrdvwwqdvisQY 340
Cdd:cd17651   84 LAFMLADAGPVLVLT------------------HPALAGELAVELVAVTLLDQPGAAA--------------------GA 125
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PTSCEVEwVDAEDPLFLLYTSGSTGKPKGVL------------HTTGGYMIYTATTFKYA---FDYKSTDVYwctadcgw 405
Cdd:cd17651  126 DAEPDPA-LDADDLAYVIYTSGSTGRPKGVVmphrslanlvawQARASSLGPGARTLQFAglgFDVSVQEIF-------- 196
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsyvtyGPMLNGATVVVfegAPNY--PDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVG 483
Cdd:cd17651  197 ---------STLCAGATLVL---PPEEvrTDPPALAAWLDEQRISRVFLPTVALRALAEHGRPLGVRLA--ALRYLLTGG 262
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EP-INPSAWRWFFNVVGDSRcpISDTWWQTET---GGFMITPLPGAWPQKPGSATfPFFGVQPVIVDEKGNEIEGECSGY 559
Cdd:cd17651  263 EQlVLTEDLREFCAGLPGLR--LHNHYGPTEThvvTALSLPGDPAAWPAPPPIGR-PIDNTRVYVLDAALRPVPPGVPGE 339
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 560 LCVKGswPGAFRTLFGD----HERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLH 635
Cdd:cd17651  340 LYIGG--AGLARGYLNRpeltAERFVPDPFVPGARMYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARH 417
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 636 PQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd17651  418 PGVREAVVLAREDRPGEKRLVAYVVGDPEAPVDaAELRAAL----ATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
195-711 2.54e-39

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 152.10  E-value: 2.54e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAGFSadslaQRIVDckpnviL 274
Cdd:cd05920   38 DRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAV--PVLALPS-----HRRSE------L 104
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCNAVKRGPKTInlkaIVDAALDQsskdgvsvgicltyDNSLATTREntkwqngrdvwwqdVISQYPtscevewvdaeDP 354
Cdd:cd05920  105 SAFCAHAEAVAY----IVPDRHAG--------------FDHRALARE--------------LAESIP-----------EV 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYmIYTATTfkyafdykSTDVYWCTAD----CGWITGHSYVTYGP-----MLNGATVVV 425
Cdd:cd05920  142 ALFLLSGGTTGTPKLIPRTHNDY-AYNVRA--------SAEVCGLDQDtvylAVLPAAHNFPLACPgvlgtLLAGGRVVL 212
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 426 fegAPNyPDPGRCWDIVDKYKVsifyTAPTLVRSL-MRDDDKFVTRHSR-KSLRVLGSVGEPINPSAWRWFFNVVGdsrC 503
Cdd:cd05920  213 ---APD-PSPDAAFPLIEREGV----TVTALVPALvSLWLDAAASRRADlSSLRLLQVGGARLSPALARRVPPVLG---C 281
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTE-----------------TGGFMITPLPGAWpqkpgsatfpffgvqpvIVDEKGNEI-EGEcSGYLCVKGs 565
Cdd:cd05920  282 TLQQVFGMAEgllnytrlddpdeviihTQGRPMSPDDEIR-----------------VVDEEGNPVpPGE-EGELLTRG- 342
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 566 wPGAFRTLFgDHERYETTYFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:cd05920  343 -PYTIRGYY-RAPEHNARAFTP-DGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVA 419
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 646 IEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMvrnQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05920  420 MPDELLGERSCAFVVLRDPPPSAAQLRRFLRER---GLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
180-711 7.29e-39

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 150.81  E-value: 7.29e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:cd12117   11 PDAVAVVYG------DRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELPAE 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGicltydnslattrentkwqngrdvwwqdvisq 339
Cdd:cd12117   85 RLAFMLADAGAKVLLTDRSLAGRAGGLEVAVVIDEALDAGPAGNPAVP-------------------------------- 132
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 yptscevewVDAEDPLFLLYTSGSTGKPKGV---------LHTTGGYMIYTA-TTF----KYAFDYKSTDVYwctadcgw 405
Cdd:cd12117  133 ---------VSPDDLAYVMYTSGSTGRPKGVavthrgvvrLVKNTNYVTLGPdDRVlqtsPLAFDASTFEIW-------- 195
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsyvtyGPMLNGATVVVFEGAPnYPDPGRCWDIVDKYKVS-IFYTAPtLVRSLMRDD-DKFvtrhsrKSLRVLGSVG 483
Cdd:cd12117  196 ---------GALLNGARLVLAPKGT-LLDPDALGALIAEEGVTvLWLTAA-LFNQLADEDpECF------AGLRELLTGG 258
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EPINPSAWRWFFNVVGDSRcpISDTWWQTETGGF----MITPLPGAWPQKP-GSatfPFFGVQPVIVDEKGNEIEGECSG 558
Cdd:cd12117  259 EVVSPPHVRRVLAACPGLR--LVNGYGPTENTTFttshVVTELDEVAGSIPiGR---PIANTRVYVLDEDGRPVPPGVPG 333
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 559 YLCVKGSwpGAFRTLFGD----HERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVL 634
Cdd:cd12117  334 ELYVGGD--GLALGYLNRpaltAERFVADPFGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRA 411
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 635 HPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPySEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd12117  412 HPGVREAVVVVREDAGGDKRLVAYVVAEGALD-AAELRAFL----RERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
181-711 2.92e-37

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 147.03  E-value: 2.92e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKD-NGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:PRK08314  25 DKTAIVFYGRAI------SYRELLEEAERLAGYLQQeCGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREE 98
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILT----CNAVKRGPKTINLK-AIVDA---ALDQSSKDGVSVGicltydnsLATTRENTKWQNGRDV 331
Cdd:PRK08314  99 ELAHYVTDSGARVAIVgselAPKVAPAVGNLRLRhVIVAQysdYLPAEPEIAVPAW--------LRAEPPLQALAPGGVV 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 332 WWQDVISQYPTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSY 411
Cdd:PRK08314 171 AWKEALAAGLAPPPHT-AGPDDLAVLPYTSGTTGVPKGCMHTHRTVM-ANAVGSVLWSNSTPESVVLAVLPLFHVTGMVH 248
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 412 VTYGPMLNGATVVVF-----EGAPnypdpgrcwDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPI 486
Cdd:PRK08314 249 SMNAPIYAGATVVLMprwdrEAAA---------RLIERYRVTHWTNIPTMVVDFLASPG--LAERDLSSLRYIGGGGAAM 317
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 487 NPSAWRWFFNVVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVD-EKGNEI-EGEcSGYLCVKG 564
Cdd:PRK08314 318 PEAVAERLKELTG---LDYVEGYGLTETMAQTHSNPPDR--PKLQCLGIPTFGVDARVIDpETLEELpPGE-VGEIVVHG 391
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 565 swPGAFRTLFGDHERYETTyFKPFAGYYF--SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:PRK08314 392 --PQVFKGYWNRPEATAEA-FIEIDGKRFfrTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEAC 468
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 378548265 643 VVGIEHEVKGQGIYAFVTL---LEGVPYSEELRKslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK08314 469 VIATPDPRRGETVKAVVVLrpeARGKTTEEEIIA----WAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQL 536
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
199-713 2.95e-37

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 146.62  E-value: 2.95e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIylpML------MELpiaMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNV 272
Cdd:cd12119   27 TYAEVAERARRLANALRRLGVKPGDRVAT---LAwnthrhLEL---YYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRV 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 ILTcnavkrgpkTINLKAIVDAALDQssKDGVSVGICLTYDNSLATTRENTKWQngrdvwWQDVISQYPTSCEVEWVDAE 352
Cdd:cd12119  101 VFV---------DRDFLPLLEAIAPR--LPTVEHVVVMTDDAAMPEPAGVGVLA------YEELLAAESPEYDWPDFDEN 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGV--------LHTTGGYMiytattfKYAFDYKSTDVY-----------WCTADCGWITGHSYVT 413
Cdd:cd12119  164 TAAAICYTSGTTGNPKGVvyshrslvLHAMAALL-------TDGLGLSESDVVlpvvpmfhvnaWGLPYAAAMVGAKLVL 236
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLNGATVVvfegapnypdpgrcwDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRW 493
Cdd:cd12119  237 PGPYLDPASLA---------------ELIEREGVTFAAGVPTVWQGLLDHLEA--NGRDLSSLRRVVIGGSAVPRSLIEA 299
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FfnvvGDSRCPISDTWWQTETG--GFMITPLPG----------AWPQKPGsatFPFFGVQPVIVDEKGNEIE--GECSGY 559
Cdd:cd12119  300 F----EERGVRVIHAWGMTETSplGTVARPPSEhsnlsedeqlALRAKQG---RPVPGVELRIVDDDGRELPwdGKAVGE 372
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 560 LCVKGSW-PGAFrtlFGDHERYEttyfKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 637
Cdd:cd12119  373 LQVRGPWvTKSY---YKNDEESE----ALTEDGWLrTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPA 445
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 638 CAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd12119  446 VAEAAVIGVPHPKWGERPLAVVVLKEGAtVTAEELLEFL----ADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALRE 518
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
353-713 1.06e-36

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 143.58  E-value: 1.06e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYW-CTAdcgWITGHSYVT--YGPMLNGATVVVFega 429
Cdd:cd05941   90 DPALILYTSGTTGRPKGVVLTHAN-LAANVRALVDAWRWTEDDVLLhVLP---LHHVHGLVNalLCPLFAGASVEFL--- 162
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 PNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRD------DDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDsrc 503
Cdd:cd05941  163 PKF-DPKEVAISRLMPSITVFMGVPTIYTRLLQYyeahftDPQFARAAAAERLRLMVSGSAALPVPTLEEWEAITGH--- 238
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTETGGFMITPLPGawPQKPGSATFPFFGVQPVIVDEKGNE-IEGECSGYLCVKGswPGAFRTLFGDHERYEt 582
Cdd:cd05941  239 TLLERYGMTEIGMALSNPLDG--ERRPGTVGMPLPGVQARIVDEETGEpLPRGEVGEIQVRG--PSVFKEYWNKPEATK- 313
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 583 tyfKPFA--GYYFSGDGCSRDKDGYYWLTGRV-DDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFV 659
Cdd:cd05941  314 ---EEFTddGWFKTGDLGVVDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVV 390
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 660 TLLEGVP--YSEELRKslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05941  391 VLRAGAAalSLEELKE----WAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
193-712 1.09e-36

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 144.83  E-value: 1.09e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 193 GVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNV 272
Cdd:PRK13391  20 STGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARA 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 ILTCNAvKRGpktinlkaIVDAALDQSSKdgvsVGICLTYDNSLATTRentkWQNgrdvwWQDVISQYPtscevEWVDAE 352
Cdd:PRK13391 100 LITSAA-KLD--------VARALLKQCPG----VRHRLVLDGDGELEG----FVG-----YAEAVAGLP-----ATPIAD 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPL--FLLYTSGSTGKPKGVLHTTGGYMIYTATT----FKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLnGATVVVF 426
Cdd:PRK13391 153 ESLgtDMLYSSGTTGRPKGIKRPLPEQPPDTPLPltafLQRLWGFRSDMVYLSPAPLYHSAPQRAVMLVIRL-GGTVIVM 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 EgapnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrcPIS 506
Cdd:PRK13391 232 E----HFDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKYDLSSLEVAIHAAAPCPPQVKEQMIDWWG----PII 303
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 507 DTWW-QTETGGFMITPlPGAWPQKPGSATFPFFGVqPVIVDEKGNEIEGECSGYLCVKGSWPgaFRTLFGDHERYETTYf 585
Cdd:PRK13391 304 HEYYaATEGLGFTACD-SEEWLAHPGTVGRAMFGD-LHILDDDGAELPPGEPGTIWFEGGRP--FEYLNDPAKTAEARH- 378
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 586 kPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV 665
Cdd:PRK13391 379 -PDGTWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGV 457
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 378548265 666 PYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK13391 458 DPGPALAAELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLR 504
PRK07798 PRK07798
acyl-CoA synthetase; Validated
171-705 1.68e-36

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 144.64  E-value: 1.68e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVEAgLGDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVH- 249
Cdd:PRK07798   9 FEAVADA-VPDRVALVCG------DRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPv 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 250 -----------------SVVFAGFSADSLAQRIVDCKPnviltcnavkRGPKtinLKAIVDAAlDQSSKDGVSVGIclTY 312
Cdd:PRK07798  82 nvnyryvedelryllddSDAVALVYEREFAPRVAEVLP----------RLPK---LRTLVVVE-DGSGNDLLPGAV--DY 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 313 DNSLATTRENtkwqngrdvwwQDVIsqyPTScevewvdaEDPLFLLYTSGSTGKPKGVLHTT--------GGYMIYTATT 384
Cdd:PRK07798 146 EDALAAGSPE-----------RDFG---ERS--------PDDLYLLYTGGTTGMPKGVMWRQedifrvllGGRDFATGEP 203
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 385 fkyafdykSTDVYWCTADCGWITG----------H---SYVTYGPMLNGATVVVfegapnYP----DPGRCWDIVDKYKV 447
Cdd:PRK07798 204 --------IEDEEELAKRAAAGPGmrrfpapplmHgagQWAAFAALFSGQTVVL------LPdvrfDADEVWRTIEREKV 269
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 448 -SIFYT----APTLVRSLMRDDDKFVTrhsrkSLRVLGSVGEPINPS---AW-RWFFNVVgdsrcpISDTWWQTETGgFM 518
Cdd:PRK07798 270 nVITIVgdamARPLLDALEARGPYDLS-----SLFAIASGGALFSPSvkeALlELLPNVV------LTDSIGSSETG-FG 337
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 519 ITPLPGAWPQKPGSATFPFfGVQPVIVDEKGNEIE--GECSGYLCVKGSWPGAFrtlFGDHERYETTyFKPFAG--YYFS 594
Cdd:PRK07798 338 GSGTVAKGAVHTGGPRFTI-GPRTVVLDEDGNPVEpgSGEIGWIARRGHIPLGY---YKDPEKTAET-FPTIDGvrYAIP 412
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 595 GDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkS 674
Cdd:PRK07798 413 GDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLA---E 489
                        570       580       590
                 ....*....|....*....|....*....|.
gi 378548265 675 LVLMVRNQIGAFAAPDRIHWAPGLPKTRSGK 705
Cdd:PRK07798 490 LRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
181-719 2.22e-36

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 144.51  E-value: 2.22e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHwegNELGvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK06087  38 DKIAVV---DNHG--ASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAE 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAVKrgpKTINLKAIVDAALDQSSKDGVsVGIcltyDNSLATTRENTKWQngrdvwwqdVISQY 340
Cdd:PRK06087 113 LVWVLNKCQAKMFFAPTLFK---QTRPVDLILPLQNQLPQLQQI-VGV----DKLAPATSSLSLSQ---------IIADY 175
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNG 420
Cdd:PRK06087 176 EPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNN-ILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIG 254
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEgapnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPInPS-----AWRWff 495
Cdd:PRK06087 255 ARSVLLD----IFTPDACLALLEQQRCTCMLGATPFIYDLLNLLEKQPADLS--ALRFFLCGGTTI-PKkvareCQQR-- 325
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 496 nvvGDSRCPIsdtWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFG 575
Cdd:PRK06087 326 ---GIKLLSV---YGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRG--PNVFMGYLD 397
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHERyeTTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK06087 398 EPEL--TARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERS 475
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 656 YAFVTLLEGVpYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQL 719
Cdd:PRK06087 476 CAYVVLKAPH-HSLTLEEVVAFFSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRKDIMRRL 538
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
181-711 5.13e-36

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 141.68  E-value: 5.13e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd17643    2 EAVAVVDE------DRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVER 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd17643   76 IAFILADSGPSLLLT----------------------------------------------------------------- 90
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvDAEDPLFLLYTSGSTGKPKGVLHTTGGYM-IYTATTfkYAFDYKSTDVywctadcgWITGHSYV------- 412
Cdd:cd17643   91 ---------DPDDLAYVIYTSGSTGRPKGVVVSHANVLaLFAATQ--RWFGFNEDDV--------WTLFHSYAfdfsvwe 151
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 413 TYGPMLNGATVVVfegAPNYP--DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGsvGEPINPSA 490
Cdd:cd17643  152 IWGALLHGGRLVV---VPYEVarSPEDFARLLRDEGVTVLNQTPSAFYQLVEAADRDGRDPLALRYVIFG--GEALEAAM 226
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 491 WRWFFNVVGDSRCPISDTWWQTETGGFM----ITP--LPGAWPQKPGsATFPFFGVQpvIVDEKGNEIEGECSGYLCVKG 564
Cdd:cd17643  227 LRPWAGRFGLDRPQLVNMYGITETTVHVtfrpLDAadLPAAAASPIG-RPLPGLRVY--VLDADGRPVPPGVVGELYVSG 303
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 565 swPGAFRtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:cd17643  304 --AGVAR---GYLGRPELTAERfvanPFGGpgsrMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHP 378
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17643  379 SVRDAAVIVREDEPGDTRLVAYVVADDGAaADIAELRALL----KELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
181-711 2.10e-35

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 139.76  E-value: 2.10e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd12115   14 DAIALVCG------DESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPER 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd12115   88 LRFILEDAQARLVLT----------------------------------------------------------------- 102
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvDAEDPLFLLYTSGSTGKPKGVLHTTGGymiytATTFkyafdykstdVYWCTADCG--WITGHSYVT----- 413
Cdd:cd12115  103 ---------DPDDLAYVIYTSGSTGRPKGVAIEHRN-----AAAF----------LQWAAAAFSaeELAGVLASTsicfd 158
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 ------YGPMLNGATVVVFEGAPNYPD-PGRCwdivdkyKVSIFYTAPTLVRSLMRDDdKFVTrhsrkSLRVLGSVGEPI 486
Cdd:cd12115  159 lsvfelFGPLATGGKVVLADNVLALPDlPAAA-------EVTLINTVPSAAAELLRHD-ALPA-----SVRVVNLAGEPL 225
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 487 N----------PSAWRwFFNVVGdsrcPISDTWWQTetgGFMITPLPGAWPqkpgSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:cd12115  226 PrdlvqrlyarLQVER-VVNLYG----PSEDTTYST---VAPVPPGASGEV----SIGRPLANTQAYVLDRALQPVPLGV 293
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGswPGAFRTLFGD----HERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 632
Cdd:cd12115  294 PGELYIGG--AGVARGYLGRpgltAERFLPDPFGPGARLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAAL 371
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 633 VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd12115  372 RSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAaGLVEDLRRHL----GTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
PLN03051 PLN03051
acyl-activating enzyme; Provisional
229-712 1.21e-34

acyl-activating enzyme; Provisional


Pssm-ID: 215552 [Multi-domain]  Cd Length: 499  Bit Score: 138.41  E-value: 1.21e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 229 LPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVdaaldqsskdgVSVGI 308
Cdd:PLN03051   1 MPMTVDAVIIYLAIVLAGCVVVSVADSFSAKEIATRLDISGAKGVFTQDVVLRGGRALPLYSKV-----------VEAAP 69
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 309 CLTYDNSLATTRENTKWQNGrDVWWQDVI-SQYPTSC------EVEWVDAEDPLFLLYTSGSTGKPKGV--LHTTGgymI 379
Cdd:PLN03051  70 AKAIVLPAAGEPVAVPLREQ-DLSWCDFLgVAAAQGSvggneySPVYAPVESVTNILFSSGTTGEPKAIpwTHLSP---L 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 380 YTATTFKYAFDYKSTDVYWCTADCGWITGhSYVTYGPMLNGATVVVFEGAPNYPDPGrcwDIVDKYKVSIFYTAPTLVRS 459
Cdd:PLN03051 146 RCASDGWAHMDIQPGDVVCWPTNLGWMMG-PWLLYSAFLNGATLALYGGAPLGRGFG---KFVQDAGVTVLGLVPSIVKA 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 460 LMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRcPISDTWWQTETGGFMI--TPLPgawPQKPGSATFPF 537
Cdd:PLN03051 222 WRHTGAFAMEGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYK-PVIEYCGGTELASGYIssTLLQ---PQAPGAFSTAS 297
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 538 FGVQPVIVDEKGNEI--EGECSGYLCVKGSWPGAF-RTLFGDHERyetTYFKPFAGYYFS-------GDGCSRDKDGYYW 607
Cdd:PLN03051 298 LGTRFVLLNDNGVPYpdDQPCVGEVALAPPMLGASdRLLNADHDK---VYYKGMPMYGSKgmplrrhGDIMKRTPGGYFC 374
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 608 LTGRVDDVINVSGHRIGTAEVESALVLHPQC-AEAAVVGIEHEVKGQG---IYAFVTLLEGVPYS---EELRKSLVLMVR 680
Cdd:PLN03051 375 VQGRADDTMNLGGIKTSSVEIERACDRAVAGiAETAAVGVAPPDGGPEllvIFLVLGEEKKGFDQarpEALQKKFQEAIQ 454
                        490       500       510
                 ....*....|....*....|....*....|..
gi 378548265 681 NQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PLN03051 455 TNLNPLFKVSRVKIVPELPRNASNKLLRRVLR 486
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
197-711 3.09e-34

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 136.07  E-value: 3.09e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCkpnviltc 276
Cdd:cd05935    1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDS-------- 72
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navkrGPKTinlkAIVDAALDqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaeDPLF 356
Cdd:cd05935   73 -----GAKV----AVVGSELD-------------------------------------------------------DLAL 88
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVF-----EGAPn 431
Cdd:cd05935   89 IPYTSGTTGLPKGCMHTHFS-AAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMarwdrETAL- 166
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 432 ypdpgrcwDIVDKYKVSIFYTAPTLVRSLMrDDDKFVTRhSRKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQ 511
Cdd:cd05935  167 --------ELIEKYKVTFWTNIPTMLVDLL-ATPEFKTR-DLSSLKVLTGGGAPMPPAVAEKLLKLTG---LRFVEGYGL 233
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 512 TETggfmITPLPGAWPQKPGSATF--PFFGVQPVIVD-EKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETTYFKPF 588
Cdd:cd05935  234 TET----MSQTHTNPPLRPKLQCLgiP*FGVDARVIDiETGRELPPNEVGEIVVRG--PQIFKGYWNRPEETEESFIEIK 307
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 589 AGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV-- 665
Cdd:cd05935  308 GRRFFrTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYrg 387
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*.
gi 378548265 666 PYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05935  388 KVTEE---DIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
181-715 3.14e-34

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 138.26  E-value: 3.14e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV----------HS 250
Cdd:PRK13295  39 DKTAVTAVRLGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVlnplmpifreRE 118
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 251 V----------------VFAGFSADSLAQRIVDCKPNviltcnavkrgpktinLKAIVdaALDQSSKDGvsvgicltYDN 314
Cdd:PRK13295 119 LsfmlkhaeskvlvvpkTFRGFDHAAMARRLRPELPA----------------LRHVV--VVGGDGADS--------FEA 172
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 315 SLATTRentkWQNGRDVwwqDVISQYPTScevewvDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiytATTFKYA--FDYK 392
Cdd:PRK13295 173 LLITPA----WEQEPDA---PAILARLRP------GPDDVTQLIYTSGTTGEPKGVMHTANTLM---ANIVPYAerLGLG 236
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 393 STDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEgapnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRdddkfVTRHS 472
Cdd:PRK13295 237 ADDVILMASPMAHQTGFMYGLMMPVMLGATAVLQD----IWDPARAAELIRTEGVTFTMASTPFLTDLTR-----AVKES 307
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 473 RK---SLRVLGSVGEPINPS----AWRwffnVVGDSRCPisdTWWQTETGGFMITpLPGAWPQKpGSAT--FPFFGVQPV 543
Cdd:PRK13295 308 GRpvsSLRTFLCAGAPIPGAlverARA----ALGAKIVS---AWGMTENGAVTLT-KLDDPDER-ASTTdgCPLPGVEVR 378
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 IVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETTyfkpFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRI 623
Cdd:PRK13295 379 VVDADGAPLPAGQIGRLQVRG--CSNFGGYLKRPQLNGTD----ADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENI 452
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 624 GTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFA-APDRIHWAPGLPKTR 702
Cdd:PRK13295 453 PVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFE---EMVEFLKAQKVAKQyIPERLVVRDALPRTP 529
                        570
                 ....*....|...
gi 378548265 703 SGKIMRRILRKIA 715
Cdd:PRK13295 530 SGKIQKFRLREML 542
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
181-711 1.17e-33

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 134.68  E-value: 1.17e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd05945    6 DRPAVVEGG------RTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAER 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILtcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd05945   80 IREILDAAKPALLI------------------------------------------------------------------ 93
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYT---------------ATTFKYAFDYKSTDVYwctadCGW 405
Cdd:cd05945   94 --------ADGDDNAYIIFTSGSTGRPKGVQISHDNLVSFTnwmlsdfplgpgdvfLNQAPFSFDLSVMDLY-----PAL 160
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 ITGhsyvtygpmlnGATVVVFEGApnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEP 485
Cdd:cd05945  161 ASG-----------ATLVPVPRDA--TADPKQLFRFLAEHGITVWVSTPSFAAMCLLSPT--FTPESLPSLRHFLFCGEV 225
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 I-NPSAWRWFfnvvgdSR---CPISDTWWQTETggfMITPLPGAWPQKPGSAT------FPFFGVQPVIVDEKGNEIEGE 555
Cdd:cd05945  226 LpHKTARALQ------QRfpdARIYNTYGPTEA---TVAVTYIEVTPEVLDGYdrlpigYAKPGAKLVILDEDGRPVPPG 296
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 556 CSGYLCVKGswPGAFRTLFGDHERYETTYFkPFAGY--YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV 633
Cdd:cd05945  297 EKGELVISG--PSVSKGYLNNPEKTAAAFF-PDEGQraYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALR 373
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 634 LHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPY--SEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05945  374 QVPGVKEAVVVPKYKGEKVTELIAFVVPKPGAEAglTKAIKAEL----AERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
180-712 3.14e-33

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 134.81  E-value: 3.14e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGNElGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:PRK08008  21 GHKTALIFESSG-GVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLRE 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTcnavkrGPKTINLKAIVDAALDQSSKdgvsvGICLTydnslattRENTKWQNGRDVWWQDVISQ 339
Cdd:PRK08008 100 ESAWILQNSQASLLVT------SAQFYPMYRQIQQEDATPLR-----HICLT--------RVALPADDGVSSFTQLKAQQ 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 YPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTT-----GGYmiYTAttFKYAFDYKstDVYW-----------CTADC 403
Cdd:PRK08008 161 PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHynlrfAGY--YSA--WQCALRDD--DVYLtvmpafhidcqCTAAM 234
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 404 gwitghsyvtygPMLN-GATVVVFEgapNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLM----RDDDKfvTRHSRKSLRV 478
Cdd:PRK08008 235 ------------AAFSaGATFVLLE---KY-SARAFWGQVCKYRATITECIPMMIRTLMvqppSANDR--QHCLREVMFY 296
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 479 LG-SVGEPinpSAWRWFFNVvgdsrcPISDTWWQTETGGFMITPLPGA---WPqkpgSATFPFFGVQPVIVDEKGNEIEG 554
Cdd:PRK08008 297 LNlSDQEK---DAFEERFGV------RLLTSYGMTETIVGIIGDRPGDkrrWP----SIGRPGFCYEAEIRDDHNRPLPA 363
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 555 ECSGYLCVKGSwPGafRTLF-GDHERYETT--YFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESA 631
Cdd:PRK08008 364 GEIGEICIKGV-PG--KTIFkEYYLDPKATakVLEA-DGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENI 439
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 632 LVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK08008 440 IATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEE---EFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516

                 .
gi 378548265 712 R 712
Cdd:PRK08008 517 K 517
PRK06188 PRK06188
acyl-CoA synthetase; Validated
181-712 7.90e-33

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 133.57  E-value: 7.90e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLP----MLMELPIAMLACARIGAVHSVVfagf 256
Cdd:PRK06188  27 DRPALVLG------DTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLnrpeVLMAIGAAQLAGLRRTALHPLG---- 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SADSLAQRIVDCKPNViLTCNAVKRGPKTINLKAIVDaaldqSSKDGVSVGICltydnslattrentkwQNGRDVWwqDV 336
Cdd:PRK06188  97 SLDDHAYVLEDAGIST-LIVDPAPFVERALALLARVP-----SLKHVLTLGPV----------------PDGVDLL--AA 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTAttfkyafdykstdvyWCTADCGWITGHSYVTYGP 416
Cdd:PRK06188 153 AAKFGPAPLVAAALPPDIAGLAYTGGTTGKPKGVMGTHRSIATMAQ---------------IQLAEWEWPADPRFLMCTP 217
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 417 ------------MLNGATVVVFEGApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGE 484
Cdd:PRK06188 218 lshaggafflptLLRGGTVIVLAKF----DPAEVLRAIEEQRITATFLVPTMIYALLDHPD--LRTRDLSSLETVYYGAS 291
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 485 PINPSAWRWFFNVVGdsrcPI-SDTWWQTETGGFmITPLP-----GAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GEcS 557
Cdd:PRK06188 292 PMSPVRLAEAIERFG----PIfAQYYGQTEAPMV-ITYLRkrdhdPDDPKRLTSCGRPTPGLRVALLDEDGREVAqGE-V 365
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 558 GYLCVKGswPGAFRtlfGDHERYETTYfKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHP 636
Cdd:PRK06188 366 GEICVRG--PLVMD---GYWNRPEETA-EAFRdGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHP 439
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 637 QCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK06188 440 AVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAA---ELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKALR 512
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
180-711 1.37e-32

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 132.40  E-value: 1.37e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:cd17646   12 PDAPAVVDEG------RTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYPAD 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTcnavkrgpktinlkaiVDAALDQSSKDGVSVGICLTYDNSLATTREntkwqngrdvwwqdvisq 339
Cdd:cd17646   86 RLAYMLADAGPAVVLT----------------TADLAARLPAGGDVALLGDEALAAPPATPP------------------ 131
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 yptscEVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATtFKYAFDYKSTDVYWCTADCG-----WitghsyVTY 414
Cdd:cd17646  132 -----LVP-PRPDNLAYVIYTSGSTGRPKGVMVTHAGIVNRLLW-MQDEYPLGPGDRVLQKTPLSfdvsvW------ELF 198
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 415 GPMLNGATVVVFEgAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfvtRHSRKSLRVLGSVGEPINPSAWRWF 494
Cdd:cd17646  199 WPLVAGARLVVAR-PGGHRDPAYLAALIREHGVTTCHFVPSMLRVFLAEPA----AGSCASLRRVFCSGEALPPELAARF 273
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 F--------NVVGDSRCPISDTWWQTEtggfmitplpGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGsw 566
Cdd:cd17646  274 LalpgaelhNLYGPTEAAIDVTHWPVR----------GPAETPSVPIGRPVPNTRLYVLDDALRPVPVGVPGELYLGG-- 341
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 567 PGAFRTLFG----DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:cd17646  342 VQLARGYLGrpalTAERFVPDPFGPGSRMYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAV 421
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 378548265 643 VVGIEHEVKGQGIYAFVTLLEG--VPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17646  422 VVARAAPAGAARLVGYVVPAAGaaGPDTAALRAHL----AERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
359-718 1.54e-32

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 129.52  E-value: 1.54e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 359 YTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGhSYVTYGPMLNGATVVVFEGAPNYPDPG-- 436
Cdd:cd05944    9 HTGGTTGTPKLAQHTHSN-EVYNAWMLALNSLFDPDDVLLCGLPLFHVNG-SVVTLLTPLASGAHVVLAGPAGYRNPGlf 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 437 -RCWDIVDKYKVSIFYTAPTLVRSLM-RDDDKFVTrhsrkSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTET 514
Cdd:cd05944   87 dNFWKLVERYRITSLSTVPTVYAALLqVPVNADIS-----SLRFAMSGAAPLPVELRARFEDATG---LPVVEGYGLTEA 158
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGFMITPLPGAwPQKPGSA--TFPFFGVQPVIVDEKGN---EIEGECSGYLCVKGswPGAFR-TLFGDHERyettyfKPF 588
Cdd:cd05944  159 TCLVAVNPPDG-PKRPGSVglRLPYARVRIKVLDGVGRllrDCAPDEVGEICVAG--PGVFGgYLYTEGNK------NAF 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 589 A--GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVP 666
Cdd:cd05944  230 VadGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAV 309
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|...
gi 378548265 667 YSEElrkSLVLMVRNQIGAFAA-PDRIHWAPGLPKTRSGKIMRRILRKIASRQ 718
Cdd:cd05944  310 VEEE---ELLAWARDHVPERAAvPKHIEVLEELPVTAVGKVFKPALRADAIHR 359
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
181-712 1.60e-32

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 131.34  E-value: 1.60e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsvvFAGFSADS 260
Cdd:cd17649    2 DAVALVFG------DQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGA----YVPLDPEY 71
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIvdckpnviltcnavkrgpktinLKAIVDAaldqsskdGVSVgicltydnslattrentkwqngrdvwwqdVISQY 340
Cdd:cd17649   72 PAERL----------------------RYMLEDS--------GAGL-----------------------------LLTHH 92
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 PtscevewvdaEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYaFDYKSTDVYWCTADCGWITGHSYVtYGPMLNG 420
Cdd:cd17649   93 P----------RQLAYVIYTSGSTGTPKGVAVSHGPLAAHCQATAER-YGLTPGDRELQFASFNFDGAHEQL-LPPLICG 160
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVfEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfVTRHSRKSLRVLGSVGEPINPSAWR-------W 493
Cdd:cd17649  161 ACVVL-RPDELWASADELAEMVRELGVTVLDLPPAYLQQLAEEADR-TGDGRPPSLRLYIFGGEALSPELLRrwlkapvR 238
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FFNVVGDSRCPISDTWWQTETGgfmitpLPGAWPQKP-GSatfPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRt 572
Cdd:cd17649  239 LFNAYGPTEATVTPLVWKCEAG------AARAGASMPiGR---PLGGRSAYILDADLNPVPVGVTGELYIGG--EGLAR- 306
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 573 lfGDHERYETT--------YFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVV 644
Cdd:cd17649  307 --GYLGRPELTaerfvpdpFGAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVV 384
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 645 GiEHEVKGQGIYAFVtLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd17649  385 A-LDGAGGKQLVAYV-VLRAAAAQPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
195-712 2.15e-32

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 131.95  E-value: 2.15e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK08276   9 GEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLI 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCNAvkrgpktinLKAIVDAALDQSSKDgvsVGICLTYDNSLATTRENTKWQNGrdvwwqdvisQYPTSCEVEWVDAEdp 354
Cdd:PRK08276  89 VSAA---------LADTAAELAAELPAG---VPLLLVVAGPVPGFRSYEEALAA----------QPDTPIADETAGAD-- 144
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 lfLLYTSGSTGKPKGVL--------HTTGGYMiytATTFKYAFDYKSTDVYWCTADcgwiTGHSYVT-YGPMLN--GATV 423
Cdd:PRK08276 145 --MLYSSGTTGRPKGIKrplpgldpDEAPGMM---LALLGFGMYGGPDSVYLSPAP----LYHTAPLrFGMSALalGGTV 215
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEGApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPinpsawrwffnvvgdsrC 503
Cdd:PRK08276 216 VVMEKF----DAEEALALIERYRVTHSQLVPTMFVRMLKLPEEVRARYDVSSLRVAIHAAAP-----------------C 274
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PIS------DtWW---------QTETGGF-MITPlpGAWPQKPGSATFPFFGVqpV-IVDEKGNEI----EG----ECSG 558
Cdd:PRK08276 275 PVEvkramiD-WWgpiiheyyaSSEGGGVtVITS--EDWLAHPGSVGKAVLGE--VrILDEDGNELppgeIGtvyfEMDG 349
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 559 YlcvkgswpgAFrTLFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQC 638
Cdd:PRK08276 350 Y---------PF-EYHNDPEKTAAARNP--HGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKV 417
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 639 AEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK08276 418 ADVAVFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLR 491
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
181-743 3.13e-32

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 134.60  E-value: 3.13e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:COG1020   491 DAVAVVFG------DQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAER 564
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  261 LAQRIVDCKPNVILTcnavkrgpktinlkaivDAALDQS-SKDGVSVgICLtyDnslattrentkwqngrdvwwQDVISQ 339
Cdd:COG1020   565 LAYMLEDAGARLVLT-----------------QSALAARlPELGVPV-LAL--D--------------------ALALAA 604
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  340 YPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVywctadCGWITGHS-----YVTY 414
Cdd:COG1020   605 EPATNPPVPVTPDDLAYVIYTSGSTGRPKGVMVEHRA-LVNLLAWMQRRYGLGPGDR------VLQFASLSfdasvWEIF 677
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  415 GPMLNGATVVVF--EGApnyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfvtRHSRKSLRVLGSVGEPINPSAWR 492
Cdd:COG1020   678 GALLSGATLVLAppEAR---RDPAALAELLARHRVTVLNLTPSLLRALLDAA-----PEALPSLRLVLVGGEALPPELVR 749
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  493 WFFNVVGDSRC-----PisdtwwqTETG----GFMITPLPGAWPQKP-GSatfPFFGVQPVIVDEKGNE----IEGEcsg 558
Cdd:COG1020   750 RWRARLPGARLvnlygP-------TETTvdstYYEVTPPDADGGSVPiGR---PIANTRVYVLDAHLQPvpvgVPGE--- 816
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  559 yLCVKGswPGafrtL----FGDH----ERYETTYFkPFAG--YYFSGDGCSRDKDG---YywlTGRVDDVINVSGHRIGT 625
Cdd:COG1020   817 -LYIGG--AG----LargyLNRPeltaERFVADPF-GFPGarLYRTGDLARWLPDGnleF---LGRADDQVKIRGFRIEL 885
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  626 AEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIgafAAPDRIHWAPGLPKTRSGK 705
Cdd:COG1020   886 GEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPY---MVPAAVVLLLPLPLTGNGK 962
                         570       580       590
                  ....*....|....*....|....*....|....*...
gi 378548265  706 IMRRILRKIASRQLEELGDTSTLADPSVVDQLIALADV 743
Cdd:COG1020   963 LDRLALPAPAAAAAAAAAAPPAEEEEEEAALALLLLLV 1000
PRK07470 PRK07470
acyl-CoA synthetase; Validated
179-724 3.22e-32

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 131.70  E-value: 3.22e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 179 LGDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHsvvfagfsa 258
Cdd:PRK07470  20 FPDRIALVWG------DRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVW--------- 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 dslaqrivdCKPNVILTCNAVkrgpktinlkaivdAALDQSSkdGVSVGICLT----YDNSLATTRENTKWQ-------N 327
Cdd:PRK07470  85 ---------VPTNFRQTPDEV--------------AYLAEAS--GARAMICHAdfpeHAAAVRAASPDLTHVvaiggarA 139
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 328 GRDVwwQDVISQYP-TSCEVEWVDAEDPLFLLYTSGSTGKPK-GVLhtTGGYMIYTATTfkyafdyKSTDVYWCT--ADC 403
Cdd:PRK07470 140 GLDY--EALVARHLgARVANAAVDHDDPCWFFFTSGTTGRPKaAVL--THGQMAFVITN-------HLADLMPGTteQDA 208
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 404 gwitghSYVTyGPMLNGATV-----------VVFEGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHS 472
Cdd:PRK07470 209 ------SLVV-APLSHGAGIhqlcqvargaaTVLLPSERF-DPAEVWALVERHRVTNLFTVPTILKMLVEHPA--VDRYD 278
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 473 RKSLRVLGSVGEPI----NPSAWRWFFNVV----GDSRCpisdtwwqteTGGfmITPLPGAW------PQ-KPGSATFPF 537
Cdd:PRK07470 279 HSSLRYVIYAGAPMyradQKRALAKLGKVLvqyfGLGEV----------TGN--ITVLPPALhdaedgPDaRIGTCGFER 346
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 538 FGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYEttyfKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVI 616
Cdd:PRK07470 347 TGMEVQIQDDEGRELPPGETGEICVIG--PAVFAGYYNNPEANA----KAFRDGWFrTGDLGHLDARGFLYITGRASDMY 420
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 617 NVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAP 696
Cdd:PRK07470 421 ISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEA---ELLAWLDGKVARYKLPKRFFFWD 497
                        570       580
                 ....*....|....*....|....*...
gi 378548265 697 GLPKTRSGKIMRRILRKiasrQLEELGD 724
Cdd:PRK07470 498 ALPKSGYGKITKKMVRE----ELEERGL 521
PRK06178 PRK06178
acyl-CoA synthetase; Validated
181-706 5.41e-31

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 128.62  E-value: 5.41e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK06178  48 QRPAIIFYGHVI------TYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHE 121
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAvkrgpktinLKAIVDAALDQSSKDGV---SVGICLTYDNSL---ATTRENTKWQNGrdvwWQ 334
Cdd:PRK06178 122 LSYELNDAGAEVLLALDQ---------LAPVVEQVRAETSLRHVivtSLADVLPAEPTLplpDSLRAPRLAAAG----AI 188
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 335 DVISQY---PTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGgYMIYTATTF-KYAFDYKSTDVYWCTADCGWITGHS 410
Cdd:PRK06178 189 DLLPALracTAPVPLPPPALDALAALNYTGGTTGMPKGCEHTQR-DMVYTAAAAyAVAVVGGEDSVFLSFLPEFWIAGEN 267
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 YVTYGPMLNGATVVVFegapNYPDPGRCWDIVDKYKVSIfytAPTLVRS---LMrdDDKFVTRHSRKSLRVLGSVG--EP 485
Cdd:PRK06178 268 FGLLFPLFSGATLVLL----ARWDAVAFMAAVERYRVTR---TVMLVDNaveLM--DHPRFAEYDLSSLRQVRVVSfvKK 338
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPsawrwffnvvgDSRcpisdTWWQTETGGFMITplpGAW--PQKPGSATF--------------PFFGVQPV------ 543
Cdd:PRK06178 339 LNP-----------DYR-----QRWRALTGSVLAE---AAWgmTETHTCDTFtagfqdddfdllsqPVFVGLPVpgtefk 399
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 IVDEKGNEI-----EGEcsgyLCV------KGSW--PGAFRTLFGDheryettyfkpfaGYYFSGDGCSRDKDGYYWLTG 610
Cdd:PRK06178 400 ICDFETGELlplgaEGE----IVVrtpsllKGYWnkPEATAEALRD-------------GWLHTGDIGKIDEQGFLHYLG 462
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 611 RVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPD 690
Cdd:PRK06178 463 RRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAA---ALQAWCRENMAVYKVPE 539
                        570
                 ....*....|....*.
gi 378548265 691 rIHWAPGLPKTRSGKI 706
Cdd:PRK06178 540 -IRIVDALPMTATGKV 554
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
198-711 9.40e-31

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 126.85  E-value: 9.40e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIvdckpnviltcn 277
Cdd:cd05923   29 LTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAELAELI------------ 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avKRGPKTINLKAiVDAALDQSSKDGvsvGICLTYDNSLATTRENTKWQngrdvwwqDVISqyPTSCEVEwvdaeDPLFL 357
Cdd:cd05923   97 --ERGEMTAAVIA-VDAQVMDAIFQS---GVRVLALSDLVGLGEPESAG--------PLIE--DPPREPE-----QPAFV 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVL---HTTGGYMIYTATTFKYAFDykSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVfegaPNYPD 434
Cdd:cd05923  156 FYTSGTTGLPKGAVipqRAAESRVLFMSTQAGLRHG--RHNVVLGLMPLYHVIGFFAVLVAALALDGTYVV----VEEFD 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 435 PGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSrkSLRVLGSVGEPINPSAWRWFFNVVGDsrcPISDTWWQTET 514
Cdd:cd05923  230 PADALKLIEQERVTSLFATPTHLDALAAAAEFAGLKLS--SLRHVTFAGATMPDAVLERVNQHLPG---EKVNIYGTTEA 304
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 515 GGFMITPLPgawpqKPGSATFPFFG--VQPVIVDEKGNEI-----EGEcsgyLCVKGSWPGAFRtlfGDHERYETTYFKP 587
Cdd:cd05923  305 MNSLYMRDA-----RTGTEMRPGFFseVRIVRIGGSPDEAlangeEGE----LIVAAAADAAFT---GYLNQPEATAKKL 372
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPY 667
Cdd:cd05923  373 QDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREGTLS 452
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 378548265 668 SEELRKslvLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd05923  453 ADELDQ---FCRASELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
198-714 2.08e-30

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 126.41  E-value: 2.08e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIltcn 277
Cdd:PRK06155  47 WTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHILRNSGARLL---- 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaIVDAALdQSSKDGVSVGicltyDNSLATTrentkWQNGRDVWWQ-----DVISQYPTSCEVEWVDAE 352
Cdd:PRK06155 123 -------------VVEAAL-LAALEAADPG-----DLPLPAV-----WLLDAPASVSvpagwSTAPLPPLDAPAPAAAVQ 178
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 --DPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTfkyafdykstdvywcTADCGWITGHSYVTYGP-------------M 417
Cdd:PRK06155 179 pgDTAAILYTSGTTGPSKGVCCPHAQFYWWGRNS---------------AEDLEIGADDVLYTTLPlfhtnalnaffqaL 243
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVV---FEGApnypdpgRCWDIVDKYKVSIFY----TAPTLVRSLMRDDDKfvtRHSrksLRV-LGSVGEPINPS 489
Cdd:PRK06155 244 LAGATYVLeprFSAS-------GFWPAVRRHGATVTYllgaMVSILLSQPARESDR---AHR---VRVaLGPGVPAALHA 310
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 490 AWRWFFNVvgdsrcPISDTWWQTETGGFMITPLPGawpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGA 569
Cdd:PRK06155 311 AFRERFGV------DLLDGYGSTETNFVIAVTHGS---QRPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADEPFA 381
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 570 FRT-LFGDHEryETTyfKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:PRK06155 382 FATgYFGMPE--KTV--EAWRNLWFhTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVP 457
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKI 714
Cdd:PRK06155 458 SELGEDEVMAAVVLRDGTALEPV---ALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQ 521
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
206-712 3.84e-30

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 124.47  E-value: 3.84e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 206 RVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQ--RIV--DCKPNViltcnavkr 281
Cdd:cd05922    2 GVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFVPLNPTLKESvlRYLvaDAGGRI--------- 72
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 282 gpktinlkAIVDAALDQSSKDGVSVgiclTYDNSLATtrentkwqnGRDVWWQDvisqyPTSCEVEWVDAEDPLFLLYTS 361
Cdd:cd05922   73 --------VLADAGAADRLRDALPA----SPDPGTVL---------DADGIRAA-----RASAPAHEVSHEDLALLLYTS 126
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 362 GSTGKPKGVL--HTTggyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGpMLNGATVVVfegAPNYPDPGRCW 439
Cdd:cd05922  127 GSTGSPKLVRlsHQN---LLANARSIAEYLGITADDRALTVLPLSYDYGLSVLNTH-LLRGATLVL---TNDGVLDDAFW 199
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 440 DIVDKYKVSIFYTAPT---LVRSLMRDDDKFvtrhsrKSLRVLGSVGEPINPSAWRWFFNVVGDSRcpISDTWWQTETGG 516
Cdd:cd05922  200 EDLREHGATGLAGVPStyaMLTRLGFDPAKL------PSLRYLTQAGGRLPQETIARLRELLPGAQ--VYVMYGQTEATR 271
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 517 FMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGD--HERYETTyfkpFAGYYFS 594
Cdd:cd05922  272 RMTYLPPERILEKPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVHRG--PNVMKGYWNDppYRRKEGR----GGGVLHT 345
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 595 GDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVkGQGIYAFVTLLEGVPYSEELRKs 674
Cdd:cd05922  346 GDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDPL-GEKLALFVTAPDKIDPKDVLRS- 423
                        490       500       510
                 ....*....|....*....|....*....|....*...
gi 378548265 675 lvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05922  424 ----LAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
181-714 5.27e-30

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 124.59  E-value: 5.27e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIhwegneLGVDASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVhSVVFagfsad 259
Cdd:PRK06839  17 DRIAI------ITEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECI-AVPL------ 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 slaqrivdckpNVILTcnavkrgpktinlkaivDAALDQSSKD-GVSVGIC-LTYDNSLATTRENTKWQngRDVWWQDV- 336
Cdd:PRK06839  84 -----------NIRLT-----------------ENELIFQLKDsGTTVLFVeKTFQNMALSMQKVSYVQ--RVISITSLk 133
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 -ISQYPTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYG 415
Cdd:PRK06839 134 eIEDRKIDNFVE-KNESASFIICYTSGTTGKPKGAVLTQEN-MFWNALNNTFAIDLTMHDRSIVLLPLFHIGGIGLFAFP 211
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 416 PMLNGATVVVfegaPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTrhsrkslrvlgsvgepiNPSAWRWFF 495
Cdd:PRK06839 212 TLFAGGVIIV----PRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETT-----------------NLQSVRWFY 270
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 496 NvvGDSRCPI-------------SDTWWQTETGG--FMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYL 560
Cdd:PRK06839 271 N--GGAPCPEelmrefidrgflfGQGFGMTETSPtvFMLSEEDAR--RKVGSIGKPVLFCDYELIDENKNKVEVGEVGEL 346
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 561 CVKGswPGAFRTLFGDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAE 640
Cdd:PRK06839 347 LIRG--PNVMKEYWNRPDATEETIQD---GWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYE 421
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 641 AAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKI 714
Cdd:PRK06839 422 VAVVGRQHVKWGEIPIAFIVKKSSSVLIEK---DVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQ 492
PRK09088 PRK09088
acyl-CoA synthetase; Validated
199-712 7.56e-30

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 124.15  E-value: 7.56e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcna 278
Cdd:PRK09088  24 TYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLLLG--- 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 279 vkrgpktinlkaivDAALDQSSKDGVSVgicltydnslattrentkwqngrDVWWQDVISQYPTscEVEWVDAEDPLFLL 358
Cdd:PRK09088 101 --------------DDAVAAGRTDVEDL-----------------------AAFIASADALEPA--DTPSIPPERVSLIL 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 359 YTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGApnypDPGRC 438
Cdd:PRK09088 142 FTSGTSGQPKGVMLSERN-LQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGF----EPKRT 216
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 439 --WDIVDKYKVSIFYTAPTLVRSLmRDDDKFVTRHSRkSLRVLGSVGEPINPSAWRWFFnvvgDSRCPISDTWWQTETGG 516
Cdd:PRK09088 217 lgRLGDPALGITHYFCVPQMAQAF-RAQPGFDAAALR-HLTALFTGGAPHAAEDILGWL----DDGIPMVDGFGMSEAGT 290
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 517 FMITPL-PGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHEryETTYFKPFAGYYFSG 595
Cdd:PRK09088 291 VFGMSVdCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRG--PNLSPGYWRRPQ--ATARAFTGDGWFRTG 366
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 596 DGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKS 674
Cdd:PRK09088 367 DIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDlERIRSH 446
                        490       500       510
                 ....*....|....*....|....*....|....*...
gi 378548265 675 LVlmvrNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK09088 447 LS----TRLAKYKVPKHLRLVDALPRTASGKLQKARLR 480
PRK13382 PRK13382
bile acid CoA ligase;
190-712 2.20e-29

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 123.33  E-value: 2.20e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 190 NELGvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAvHSVVF-AGFSADSLAQRIVDC 268
Cdd:PRK13382  64 DELG---TLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGA-DILLLnTSFAGPALAEVVTRE 139
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 269 KPNVILTCNavkrgpktiNLKAIVDAALDQSSKdgvsvgicltydnslaTTReNTKWQNGRDVWWQDVISQYPTSCEVEW 348
Cdd:PRK13382 140 GVDTVIYDE---------EFSATVDRALADCPQ----------------ATR-IVAWTDEDHDLTVEVLIAAHAGQRPEP 193
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 349 VDAEDPLFLLyTSGSTGKPKGVLHT-TGGYMIYTATTFKYAfdykstdvywctadcgWITGHSYVTYGPMLN--GATVVV 425
Cdd:PRK13382 194 TGRKGRVILL-TSGTTGTPKGARRSgPGGIGTLKAILDRTP----------------WRAEEPTVIVAPMFHawGFSQLV 256
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 426 FEGAPNYP-------DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVV 498
Cdd:PRK13382 257 LAASLACTivtrrrfDPEATLDLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQF 336
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 GDSrcpISDTWWQTETGgfMI-TPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEI-EGECsGYLCVKGSwpgafrTLFgd 576
Cdd:PRK13382 337 GDV---IYNNYNATEAG--MIaTATPADLRAAPDTAGRPAEGTEIRILDQDFREVpTGEV-GTIFVRND------TQF-- 402
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 577 hERYETTYFKPF-AGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK13382 403 -DGYTSGSTKDFhDGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRL 481
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 656 YAFVTLLEGVPYSEELRKSlvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK13382 482 AAFVVLKPGASATPETLKQ---HVRDNLANYKVPRDIVVLDELPRGATGKILRRELQ 535
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
181-721 3.18e-29

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 123.22  E-value: 3.18e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK06710  39 EKKALHFLGKDI------TFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERE 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAVKrgPKTINLKAI--VDAALDQSSKDGVSVGICLTYDnSLATTREN--TKWQNGRDV-WWQD 335
Cdd:PRK06710 113 LEYQLHDSGAKVILCLDLVF--PRVTNVQSAtkIEHVIVTRIADFLPFPKNLLYP-FVQKKQSNlvVKVSESETIhLWNS 189
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 336 VISQYPTSCEVEwVDAEDPLFLL-YTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKS-TDVYWCTADCGWITGHSYVT 413
Cdd:PRK06710 190 VEKEVNTGVEVP-CDPENDLALLqYTGGTTGFPKGVMLTHKNLVSNTLMGVQWLYNCKEgEEVVLGVLPFFHVYGMTAVM 268
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLNGATVVVFegaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMrdDDKFVTRHSRKSLRVLGSVGEPINPSAWRW 493
Cdd:PRK06710 269 NLSIMQGYKMVLI---PKF-DMKMVFEAIKKHKVTLFPGAPTIYIALL--NSPLLKEYDISSIRACISGSAPLPVEVQEK 342
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FFNVVGDSrcpISDTWWQTETGGfmITPLPGAWPQK-PGSATFPFFGVQPVIVD-EKGNEIEGECSGYLCVKGSwpgafR 571
Cdd:PRK06710 343 FETVTGGK---LVEGYGLTESSP--VTHSNFLWEKRvPGSIGVPWPDTEAMIMSlETGEALPPGEIGEIVVKGP-----Q 412
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 572 TLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVK 651
Cdd:PRK06710 413 IMKGYWNKPEETAAVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYR 492
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 652 GQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEE 721
Cdd:PRK06710 493 GETVKAFVVLKEGTECSEE---ELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIEEEKRKNED 559
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
180-713 8.50e-29

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 120.87  E-value: 8.50e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWegnelgVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYL---PMLMELPIAMLACariGAVHSVVFAGF 256
Cdd:cd12118   18 PDRTSIVY------GDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLApntPAMYELHFGVPMA---GAVLNALNTRL 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SADSLAQRIVDCKPNVILTcnavkrgpktinlkaivdaalDQSskdgvsvgicLTYDNSLATTRENTKWQngrdvWWQDv 336
Cdd:cd12118   89 DAEEIAFILRHSEAKVLFV---------------------DRE----------FEYEDLLAEGDPDFEWI-----PPAD- 131
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 isqyptscevEWvdaeDPLFLLYTSGSTGKPKGVLHT-TGGYMIYTATTfkYAFDYKSTDVYWCTAD----CGWItghsy 411
Cdd:cd12118  132 ----------EW----DPIALNYTSGTTGRPKGVVYHhRGAYLNALANI--LEWEMKQHPVYLWTLPmfhcNGWC----- 190
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 412 VTYGPMLNGATVVVFEGApnypDPGRCWDIVDKYKVSIFYTAPTlVRSLMRDDDKFVTRHSRKSLRVLgSVGEPINPS-- 489
Cdd:cd12118  191 FPWTVAAVGGTNVCLRKV----DAKAIYDLIEKHKVTHFCGAPT-VLNMLANAPPSDARPLPHRVHVM-TAGAPPPAAvl 264
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 490 --AWRWFFNVVgdsrcpisDTWWQTETGGFMITplpGAWpqKPGSATFP--------------FFGVQPVIVDEK----- 548
Cdd:cd12118  265 akMEELGFDVT--------HVYGLTETYGPATV---CAW--KPEWDELPteerarlkarqgvrYVGLEEVDVLDPetmkp 331
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 549 ----GNEIeGEC--SGYLCVKGSW--PGAFRTLFGDheryettyfkpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSG 620
Cdd:cd12118  332 vprdGKTI-GEIvfRGNIVMKGYLknPEATAEAFRG-------------GWFHSGDLAVIHPDGYIEIKDRSKDIIISGG 397
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 621 HRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPgLPK 700
Cdd:cd12118  398 ENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEE---EIIAFCREHLAGFMVPKTVVFGE-LPK 473
                        570
                 ....*....|...
gi 378548265 701 TRSGKIMRRILRK 713
Cdd:cd12118  474 TSTGKIQKFVLRD 486
PRK07788 PRK07788
acyl-CoA synthetase; Validated
195-714 1.73e-28

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 120.80  E-value: 1.73e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAqrivdckpnvil 274
Cdd:PRK07788  72 RGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQLA------------ 139
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 tcnavkrgpktinlkaivdaalDQSSKDGVSVgicLTYDNSLATTRENTKWQNGR-DVWWQDVISQYPTSCEVEWVD--- 350
Cdd:PRK07788 140 ----------------------EVAAREGVKA---LVYDDEFTDLLSALPPDLGRlRAWGGNPDDDEPSGSTDETLDdli 194
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 ---AEDPL--------FLLYTSGSTGKPKGVLH------TTGGyMIYTATTFKyafdykSTDVYWCTADCGWITGHSYVT 413
Cdd:PRK07788 195 agsSTAPLpkppkpggIVILTSGTTGTPKGAPRpepsplAPLA-GLLSRVPFR------AGETTLLPAPMFHATGWAHLT 267
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLnGATVVV---FegapnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSA 490
Cdd:PRK07788 268 LAMAL-GSTVVLrrrF-------DPEATLEDIAKHKATALVVVPVMLSRILDLGPEVLAKYDTSSLKIIFVSGSALSPEL 339
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 491 WRWFFNVVGDSRCpisDTWWQTETGgFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPgaf 570
Cdd:PRK07788 340 ATRALEAFGPVLY---NLYGSTEVA-FATIATPEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFP--- 412
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 rtlFgdhERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHE 649
Cdd:PRK07788 413 ---F---EGYTDGRDKQIIdGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDE 486
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 650 VKGQGIYAFVTLLEGVPYSEELRKSlvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKI 714
Cdd:PRK07788 487 EFGQRLRAFVVKAPGAALDEDAIKD---YVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREM 548
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
181-711 5.48e-28

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 118.14  E-value: 5.48e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHwegnelGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd12114    2 DATAVI------CGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAAR 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKDGVSVGicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd12114   76 REAILADAGARLVLTDGPDAQLDVAVFDVLILDLDALAAPAPPPPVD--------------------------------- 122
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYM-IYTATTFKYA--------------FDYKSTDVYwctadcgw 405
Cdd:cd12114  123 --------VAPDDLAYVIFTSGSTGTPKGVMISHRAALnTILDINRRFAvgpddrvlalsslsFDLSVYDIF-------- 186
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsyvtyGPMLNGATVVVFEGAPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMrDDDKFVTRHSRkSLR-VLGSvGE 484
Cdd:cd12114  187 ---------GALSAGATLVLPDEARR-RDPAHWAELIERHGVTLWNSVPALLEMLL-DVLEAAQALLP-SLRlVLLS-GD 253
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 485 PIN---PSAWRWFF---NVV---GDSRCPISDTWWQtetggfmITPLPGAWPQKP-GsatFPFFGVQPVIVDEKGNEIEG 554
Cdd:cd12114  254 WIPldlPARLRALApdaRLIslgGATEASIWSIYHP-------IDEVPPDWRSIPyG---RPLANQRYRVLDPRGRDCPD 323
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 555 ECSGYLCVKGswPGAFRTLFGDHERYETTYFKPFAG--YYFSGD-GCSRDkDGYYWLTGRVDDVINVSGHRIGTAEVESA 631
Cdd:cd12114  324 WVPGELWIGG--RGVALGYLGDPELTAARFVTHPDGerLYRTGDlGRYRP-DGTLEFLGRRDGQVKVRGYRIELGEIEAA 400
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 632 LVLHPQCAEAAVVGIEhEVKGQGIYAFVTLLEGVP--YSEELRKSLVLMVRnqigAFAAPDRIHWAPGLPKTRSGKIMRR 709
Cdd:cd12114  401 LQAHPGVARAVVVVLG-DPGGKRLAAFVVPDNDGTpiAPDALRAFLAQTLP----AYMIPSRVIALEALPLTANGKVDRA 475

                 ..
gi 378548265 710 IL 711
Cdd:cd12114  476 AL 477
PRK06145 PRK06145
acyl-CoA synthetase; Validated
181-712 7.44e-28

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 118.06  E-value: 7.44e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELGvdasltYSELLQRVCQLANYLKDNGVKKGDAVVIYL---PMLMELpiaMLACARIGAVHSVVFAGFS 257
Cdd:PRK06145  17 DRAALVYRDQEIS------YAEFHQRILQAAGMLHARGIGQGDVVALLMknsAAFLEL---AFAASYLGAVFLPINYRLA 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 258 ADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVDAALDQSSKdgvsvgicltydnslattrentkwqngrdvwwqdVI 337
Cdd:PRK06145  88 ADEVAYILGDAGAKLLLVDEEFDAIVALETPKIVIDAAAQADSR----------------------------------RL 133
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 338 SQYPTSCEVEWVDAEDPLF-LLYTSGSTGKPKGVLHTTGGymiytattfkyaFDYKSTDVywcTADCGWITGHSYVTYGP 416
Cdd:PRK06145 134 AQGGLEIPPQAAVAPTDLVrLMYTSGTTDRPKGVMHSYGN------------LHWKSIDH---VIALGLTASERLLVVGP 198
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 417 M-------LNGATVVVFEGA----PNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFvtRHSRKSLRVLGSVGEP 485
Cdd:PRK06145 199 LyhvgafdLPGIAVLWVGGTlrihREF-DPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRD--RFDLDSLAWCIGGGEK 275
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFFNVVGDSRcpISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGs 565
Cdd:PRK06145 276 TPESRIRDFTRVFTRAR--YIDAYGLTETCSGDTLMEAGREIEKIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRG- 352
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 566 wPGAFRTLFGDHERYETTYFkpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:PRK06145 353 -PKVTKGYWKDPEKTAEAFY---GDWFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIG 428
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 646 IEHEVKGQGIYAFVTLLEGVPYS-EELRKSlvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK06145 429 VHDDRWGERITAVVVLNPGATLTlEALDRH----CRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLR 492
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
351-712 9.96e-28

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 117.80  E-value: 9.96e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 AEDPL--FLLYTSGSTGKPKGV--------LHTTGGYMIYTATTFkyaFDYKSTDVYWCTADcgwITGHSYVTYGPMLN- 419
Cdd:PRK13390 145 TEQPCgaVMLYSSGTTGFPKGIqpdlpgrdVDAPGDPIVAIARAF---YDISESDIYYSSAP---IYHAAPLRWCSMVHa 218
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 -GATVVVfegAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVV 498
Cdd:PRK13390 219 lGGTVVL---AKRF-DAQATLGHVERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRAVIHAAAPCPVDVKHAMIDWL 294
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 GdsrcPISDTWWQ-TETGGFMITPLPgAWPQKPGSATFPFFGVQPvIVDEKGNEI-EGECSGYLCVKGSWPgaFRTLFGD 576
Cdd:PRK13390 295 G----PIVYEYYSsTEAHGMTFIDSP-DWLAHPGSVGRSVLGDLH-ICDDDGNELpAGRIGTVYFERDRLP--FRYLNDP 366
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 577 HERYETTY-FKPFagYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK13390 367 EKTAAAQHpAHPF--WTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQV 444
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 656 YAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK13390 445 KAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
AMP-binding_C pfam13193
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ...
627-705 4.38e-27

AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.


Pssm-ID: 463804 [Multi-domain]  Cd Length: 76  Bit Score: 104.55  E-value: 4.38e-27
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 378548265  627 EVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVpysEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGK 705
Cdd:pfam13193   1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGV---ELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
181-713 9.15e-27

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 115.26  E-value: 9.15e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK07786  32 DAPALRFLGN------TTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPE 105
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILT-------CNAVKRGPKTINLKAIVDAAldqsSKDGVsvgicLTYDNSLAttrentkwQNGRDVWW 333
Cdd:PRK07786 106 IAFLVSDCGAHVVVTeaalapvATAVRDIVPLLSTVVVAGGS----SDDSV-----LGYEDLLA--------EAGPAHAP 168
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 334 QDVisqyptscevewvdAED-PLFLLYTSGSTGKPKG-VL-HTTggyMIYTATTFKYAFDY-KSTDVYWCTADCGWITGH 409
Cdd:PRK07786 169 VDI--------------PNDsPALIMYTSGTTGRPKGaVLtHAN---LTGQAMTCLRTNGAdINSDVGFVGVPLFHIAGI 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 410 SYVTYGPMLNGATVVVFEGAPnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfvTRHSRKSLRVLGSVGEPINPS 489
Cdd:PRK07786 232 GSMLPGLLLGAPTVIYPLGAF---DPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQ---ARPRDLALRVLSWGAAPASDT 305
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 490 AWRWFFNVVGDSRcpISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpga 569
Cdd:PRK07786 306 LLRQMAATFPEAQ--ILAAFGQTEMSPVTCMLLGEDAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAP---- 379
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 570 frTLFGDHERYETTYFKPFAGYYF-SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEH 648
Cdd:PRK07786 380 --TLMSGYWNNPEATAEAFAGGWFhSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRAD 457
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 649 EVKGQGIYAFVTLLEGvpySEELR-KSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK07786 458 EKWGEVPVAVAAVRND---DAALTlEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRE 520
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
357-712 1.34e-26

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 114.01  E-value: 1.34e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDykstdvywctadCGWITGHSYVTYGPM-------------LNGATV 423
Cdd:cd05929  130 MLYSGGTTGRPKGIKRGLPGGPPDNDTLMAAALG------------FGPGADSVYLSPAPLyhaapfrwsmtalFMGGTL 197
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEgapNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPinpsawrwffnvvgdsrC 503
Cdd:cd05929  198 VLME---KF-DPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEAVRNAYDLSSLKRVIHAAAP-----------------C 256
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PIS-----DTWW---------QTETGGFmiTPLPGA-WPQKPGSATFPFFGVQPvIVDEKGNEIEGECSGYLCVKGSWPg 568
Cdd:cd05929  257 PPWvkeqwIDWGgpiiweyygGTEGQGL--TIINGEeWLTHPGSVGRAVLGKVH-ILDEDGNEVPPGEIGEVYFANGPG- 332
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 afrtlFGDHERYETTYFKPFAGYYFS-GDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:cd05929  333 -----FEYTNDPEKTAAARNEGGWSTlGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVP 407
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05929  408 DEELGQRVHAVVQPAPGADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
195-712 1.61e-26

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 114.03  E-value: 1.61e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVI- 273
Cdd:PRK12406   9 DRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLi 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 274 ----LTCNAVKRGPKTINLKAIVDAALDQSSkdgvsVGIcltyDNSLATTRENTkwqngrdVWWQDVISQYPTScevEWV 349
Cdd:PRK12406  89 ahadLLHGLASALPAGVTVLSVPTPPEIAAA-----YRI----SPALLTPPAGA-------IDWEGWLAQQEPY---DGP 149
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGgymiyTATTFKYAFDYKSTdVYwctadcGWITGHSYVTYGPMLNGA-------- 421
Cdd:PRK12406 150 PVPQPQSMIYTSGTTGHPKGVRRAAP-----TPEQAAAAEQMRAL-IY------GLKPGIRALLTGPLYHSApnayglra 217
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 422 ----TVVVFEgaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWR----W 493
Cdd:PRK12406 218 grlgGVLVLQ--PRF-DPEELLQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHAAAPCPADVKRamieW 294
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 FFNVvgdsrcpISDTWWQTETGGfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAfrTL 573
Cdd:PRK12406 295 WGPV-------IYEYYGSTESGA-VTFATSEDALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRI--AGN--PD 362
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:PRK12406 363 FTYHNKPEKRAEIDRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGE 442
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 654 GIYAFVTLLEGVPYSEE-LRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK12406 443 ALMAVVEPQPGATLDEAdIRAQL----KARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
PLN02574 PLN02574
4-coumarate--CoA ligase-like
197-714 2.55e-26

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 114.17  E-value: 2.55e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPnvilt 275
Cdd:PLN02574  66 SISYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSV----- 140
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 cnavkrgpktinlkAIVDAALDQSSKDGvSVGIcltydnSLATTRENTKWQNGRDVW--WQDVISQYPTSCEVEWVDAED 353
Cdd:PLN02574 141 --------------GLAFTSPENVEKLS-PLGV------PVIGVPENYDFDSKRIEFpkFYELIKEDFDFVPKPVIKQDD 199
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLHTTGGY--MIYTATTFKYA-FDYKSTD-VYWCTADCGWITGHSYVTYGPMLNGATVVVFEGA 429
Cdd:PLN02574 200 VAAIMYSSGTTGASKGVVLTHRNLiaMVELFVRFEASqYEYPGSDnVYLAALPMFHIYGLSLFVVGLLSLGSTIVVMRRF 279
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 pnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVgdSRCPISDTW 509
Cdd:PLN02574 280 ----DASDMVKVIDRFKVTHFPVVPPILMALTKKA-KGVCGEVLKSLKQVSCGAAPLSGKFIQDFVQTL--PHVDFIQGY 352
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 510 WQTE-----TGGFMITPLpgawpQKPGSATFPFFGVQPVIVD-EKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETT 583
Cdd:PLN02574 353 GMTEstavgTRGFNTEKL-----SKYSSVGLLAPNMQAKVVDwSTGCLLPPGNCGELWIQG--PGVMKGYLNNPKATQST 425
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 584 YFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLE 663
Cdd:PLN02574 426 IDKD--GWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQ 503
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|.
gi 378548265 664 GVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKI 714
Cdd:PLN02574 504 GSTLSQE---AVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKRS 551
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
181-718 9.07e-26

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 111.59  E-value: 9.07e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAG--FSA 258
Cdd:PRK03640  17 DRTAIEFE------EKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAV--AVLLNtrLSR 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 DSLAQRIVDCKPNVILTcnavkrgpktinlkaivdaalDQSSKDGVSVGICLTYDNSLATTRENTKWQngrdvwwqdvis 338
Cdd:PRK03640  89 EELLWQLDDAEVKCLIT---------------------DDDFEAKLIPGISVKFAELMNGPKEEAEIQ------------ 135
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 339 qyptscevEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPML 418
Cdd:PRK03640 136 --------EEFDLDEVATIMYTSGTTGKPKGVIQTYGNHW-WSAVGSALNLGLTEDDCWLAAVPIFHISGLSILMRSVIY 206
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 nGATVVVFEGApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtRHSRKSLR--VLGsvGEPINPSawrwFFN 496
Cdd:PRK03640 207 -GMRVVLVEKF----DAEKINKLLQTGGVTIISVVSTMLQRLLERLGE---GTYPSSFRcmLLG--GGPAPKP----LLE 272
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 VVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEkGNEIEGECSGYLCVKGS--WPGAFRtlf 574
Cdd:PRK03640 273 QCKEKGIPVYQSYGMTETASQIVTLSPEDALTKLGSAGKPLFPCELKIEKD-GVVVPPFEEGEIVVKGPnvTKGYLN--- 348
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 gdheRYETTYfKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:PRK03640 349 ----REDATR-ETFQdGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQ 423
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 654 GIYAFVTLLEGVPySEELRkslvLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQ 718
Cdd:PRK03640 424 VPVAFVVKSGEVT-EEELR----HFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQLVEEM 483
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
181-711 1.22e-25

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 111.27  E-value: 1.22e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd17655   12 DHTAVVFE------DQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDYPEER 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTCNAVKrgPKTINLKAIVDAALDQSSKDGVSvgicltydnSLAttrentkwqngrdvwwqdVISQy 340
Cdd:cd17655   86 IQYILEDSGADILLTQSHLQ--PPIAFIGLIDLLDEDTIYHEESE---------NLE------------------PVSK- 135
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvdAEDPLFLLYTSGSTGKPKGVLHTTGG-----------YMIYTATTF----KYAFDYKSTDVYwctadcgw 405
Cdd:cd17655  136 ----------SDDLAYVIYTSGSTGKPKGVMIEHRGvvnlvewankvIYQGEHLRValfaSISFDASVTEIF-------- 197
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsyvtyGPMLNGATVVVFEGAPNYPDPGRCwDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhSRKSLRVLGSVGEP 485
Cdd:cd17655  198 ---------ASLLSGNTLYIVRKETVLDGQALT-QYIRQNRITIIDLTPAHLKLLDAADDS-----EGLSLKHLIVGGEA 262
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFFNVVGDSrCPISDTWWQTETG-GFMITPLPGAWPQKP----GSatfPFFGVQPVIVDEKGNEIEGECSGYL 560
Cdd:cd17655  263 LSTELAKKIIELFGTN-PTITNAYGPTETTvDASIYQYEPETDQQVsvpiGK---PLGNTRIYILDQYGRPQPVGVAGEL 338
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 561 CVKGSwpGAFRtlfGDHERYETTYFK----PFAG---YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV 633
Cdd:cd17655  339 YIGGE--GVAR---GYLNRPELTAEKfvddPFVPgerMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLL 413
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 634 LHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPySEELRKSLvlmvRNQIGAFAAP------DRIhwapglPKTRSGKIM 707
Cdd:cd17655  414 QHPDIKEAVVIARKDEQGQNYLCAYIVSEKELP-VAQLREFL----ARELPDYMIPsyfiklDEI------PLTPNGKVD 482

                 ....
gi 378548265 708 RRIL 711
Cdd:cd17655  483 RKAL 486
PRK07638 PRK07638
acyl-CoA synthetase; Validated
181-720 2.89e-25

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 110.25  E-value: 2.89e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGvKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK07638  16 NKIAIKEN------DRVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDE 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcNAVKRGPKTINLKAIVDaaLDQsskdgvsvgicltydnslattrentkwqngrdvwWQDVISQY 340
Cdd:PRK07638  89 LKERLAISNADMIVT-ERYKLNDLPDEEGRVIE--IDE----------------------------------WKRMIEKY 131
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 -PTSCEVEwvDAE-DPLFLLYTSGSTGKPKGVLHTTGGYMiytattfkYAFDYKSTDVYWCTADCGWITG---HSYVTYG 415
Cdd:PRK07638 132 lPTYAPIE--NVQnAPFYMGFTSGSTGKPKAFLRAQQSWL--------HSFDCNVHDFHMKREDSVLIAGtlvHSLFLYG 201
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 416 P---MLNGATVVVFegaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDD------DKFVT------RHSRKSLRVlg 480
Cdd:PRK07638 202 AistLYVGQTVHLM---RKF-IPNQVLDKLETENISVMYTVPTMLESLYKENrvienkMKIISsgakweAEAKEKIKN-- 275
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 481 svgepINPSAWRWFFnvvgdsrcpisdtwWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYL 560
Cdd:PRK07638 276 -----IFPYAKLYEF--------------YGASELSFVTALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTV 336
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 561 CVKGswPGAFRTLFGDHERYETTyfkPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAE 640
Cdd:PRK07638 337 YVKS--PQFFMGYIIGGVLAREL---NADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDE 411
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 641 AAVVGIEHEVKGQGIYAFVtllEGVPYSEELRKslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLE 720
Cdd:PRK07638 412 IVVIGVPDSYWGEKPVAII---KGSATKQQLKS----FCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWIENQEK 484
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
349-713 6.01e-25

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 108.20  E-value: 6.01e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 349 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGpMLNGATVVVFEG 428
Cdd:cd05912   74 VKLDDIATIMYTSGTTGKPKGVQQTFGNHW-WSAIGSALNLGLTEDDNWLCALPLFHISGLSILMRS-VIYGMTVYLVDK 151
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 429 ApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLrvLGsvGEPINPSawrwFFNVVGDSRCPISDT 508
Cdd:cd05912  152 F----DAEQVLHLINSGKVTIISVVPTMLQRLLEILGEGYPNNLRCIL--LG--GGPAPKP----LLEQCKEKGIPVYQS 219
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 509 WWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GEcsgyLCVKGS--WPGAFRTLFGDHERYETTYF 585
Cdd:cd05912  220 YGMTETCSQIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQPPYEvGE----ILLKGPnvTKGYLNRPDATEESFENGWF 295
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 586 KpfagyyfSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEgv 665
Cdd:cd05912  296 K-------TGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSER-- 366
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*...
gi 378548265 666 PYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05912  367 PISEE---ELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
198-711 6.27e-25

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 108.71  E-value: 6.27e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcn 277
Cdd:cd17650   13 LTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYMLEDSGAKLLLT-- 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDPLFL 357
Cdd:cd17650   91 ------------------------------------------------------------------------QPEDLAYV 98
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGYM-IYTATTFKYAFDYK----------STDVYwctadcgwiTGHSYVTygpMLNGATVVVf 426
Cdd:cd17650   99 IYTSGTTGKPKGVMVEHRNVAhAAHAWRREYELDSFpvrllqmasfSFDVF---------AGDFARS---LLNGGTLVI- 165
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 egAPN--YPDPGRCWDIVDKYKVSIFYTAPTLVRSLMrdddKFVTRHSRK--SLRVL--GSVGEPINPSAWRW------- 493
Cdd:cd17650  166 --CPDevKLDPAALYDLILKSRITLMESTPALIRPVM----AYVYRNGLDlsAMRLLivGSDGCKAQDFKTLAarfgqgm 239
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 -FFNVVGDSRCPISDTWWQTETGgfmitPLPGAWPQKPGSatfPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRT 572
Cdd:cd17650  240 rIINSYGVTEATIDSTYYEEGRD-----PLGDSANVPIGR---PLPNTAMYVLDERLQPQPVGVAGELYIGGA--GVARG 309
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 573 LFGD----HERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVgIEH 648
Cdd:cd17650  310 YLNRpeltAERFVENPFAPGERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVA-VRE 388
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 649 EVKGQ-GIYAFVTLLEgVPYSEELRKSLVlmvrNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17650  389 DKGGEaRLCAYVVAAA-TLNTAELRAFLA----KELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
PRK06164 PRK06164
acyl-CoA synthetase; Validated
192-721 6.46e-25

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 109.45  E-value: 6.46e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 192 LGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPN 271
Cdd:PRK06164  30 IDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRGRAR 109
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 272 VIltcnAVKRGPKTINLKAIVDAALDQSSKD--GVSVgICLTYDNSLATTRentkwqnGRDVWWQDVISQYPTSCEVEWV 349
Cdd:PRK06164 110 WL----VVWPGFKGIDFAAILAAVPPDALPPlrAIAV-VDDAADATPAPAP-------GARVQLFALPDPAPPAAAGERA 177
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLY-TSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTAD-CGwITGHSYVTyGPMLNGATVV--- 424
Cdd:PRK06164 178 ADPDAGALLFtTSGTTSGPKLVLHRQAT-LLRHARAIARAYGYDPGAVLLAALPfCG-VFGFSTLL-GALAGGAPLVcep 254
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 425 VFEGApnypdpgRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtRHSRKSLRVLGSVGepiNPSAWRWFFNVVGDSRCP 504
Cdd:PRK06164 255 VFDAA-------RTARALRRHRVTHTFGNDEMLRRILDTAGE---RADFPSARLFGFAS---FAPALGELAALARARGVP 321
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 ISDTWWQTE-----TGGFMITP-----LPGAWPQKPgsatfpffGVQPVIVDEKGNEI--EGEcSGYLCVKGswPGAFRT 572
Cdd:PRK06164 322 LTGLYGSSEvqalvALQPATDPvsvriEGGGRPASP--------EARVRARDPQDGALlpDGE-SGEIEIRA--PSLMRG 390
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 573 LFGDHERYETTyFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEvkG 652
Cdd:PRK06164 391 YLDNPDATARA-LTD-DGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGATRD--G 466
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 653 QGI-YAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSG---KIMRRILRKIASRQLEE 721
Cdd:PRK06164 467 KTVpVAFVIPTDGASPDEA---GLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLREMAQARLAA 536
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
353-715 6.74e-25

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 106.26  E-value: 6.74e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHsYVTYGPMLNGATVVVFEGAP-- 430
Cdd:cd17630    1 RLATVILTSGSTGTPKAVVHTAAN-LLASAAGLHSRLGFGGGDSWLLSLPLYHVGGL-AILVRSLLAGAELVLLERNQal 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 431 --NYPDPGRCWdivdkykVSIfytAPTLVRSLMRDDdkfVTRHSRKSLRVLGSVGEPINPSAWRWFfnvvGDSRCPISDT 508
Cdd:cd17630   79 aeDLAPPGVTH-------VSL---VPTQLQRLLDSG---QGPAALKSLRAVLLGGAPIPPELLERA----ADRGIPLYTT 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 509 WWQTETGGfMITPLPGAWPQKPGSATfPFFGVQPVIVDEkgneiegecsGYLCVKGswpgafRTLFGDHERYETTYFKPF 588
Cdd:cd17630  142 YGMTETAS-QVATKRPDGFGRGGVGV-LLPGRELRIVED----------GEIWVGG------ASLAMGYLRGQLVPEFNE 203
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 589 AGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPyS 668
Cdd:cd17630  204 DGWFTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPAD-P 282
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*..
gi 378548265 669 EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:cd17630  283 AELRAWL----KDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
353-708 1.19e-24

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 105.57  E-value: 1.19e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGYMiytattfkYAFDYKSTDVYWCTADCGWITG---HSYVTYGPM---LNGATVVVF 426
Cdd:cd17633    1 NPFYIGFTSGTTGLPKAYYRSERSWI--------ESFVCNEDLFNISGEDAILAPGplsHSLFLYGAIsalYLGGTFIGQ 72
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 EGApnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfVTRHSRKSLRvlgSVGEPINPSAWRWFFNVVGDSRcpIS 506
Cdd:cd17633   73 RKF----NPKSWIRKINQYNATVIYLVPTMLQALARTL---EPESKIKSIF---SSGQKLFESTKKKLKNIFPKAN--LI 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 507 DTWWQTETGgfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIegecsGYLCVKGswPGAFrtlfgdhERYETTYFK 586
Cdd:cd17633  141 EFYGTSELS--FITYNFNQESRPPNSVGRPFPNVEIEIRNADGGEI-----GKIFVKS--EMVF-------SGYVRGGFS 204
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 587 PFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQgIYAFVTLLEGVP 666
Cdd:cd17633  205 NPDGWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGE-IAVALYSGDKLT 283
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|..
gi 378548265 667 YSEELRKSLVLMVRNQIgafaaPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17633  284 YKQLKRFLKQKLSRYEI-----PKKIIFVDSLPYTSSGKIAR 320
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
195-711 1.34e-24

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 107.34  E-value: 1.34e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd17652   10 DETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLADARPALLL 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDP 354
Cdd:cd17652   90 T--------------------------------------------------------------------------TPDNL 95
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYK-----------STDVY---WCTAdcgwitghsyvtygpMLNG 420
Cdd:cd17652   96 AYVIYTSGSTGRPKGVVVTHRG-LANLAAAQIAAFDVGpgsrvlqfaspSFDASvweLLMA---------------LLAG 159
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEGAPNYPDPGRCwDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhsrkSLRVLGSVGEPINPS-AWRW-----F 494
Cdd:cd17652  160 ATLVLAPAEELLPGEPLA-DLLREHRITHVTLPPAALAALPPDDLP--------DLRTLVVAGEACPAElVDRWapgrrM 230
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 FNVVGDSRCPISDTWWQTETGGFMItPLPGAWPqkpgsatfpffGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRtlf 574
Cdd:cd17652  231 INAYGPTETTVCATMAGPLPGGGVP-PIGRPVP-----------GTRVYVLDARLRPVPPGVPGELYIAG--AGLAR--- 293
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 GDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGI 646
Cdd:cd17652  294 GYLNRPGLTAERfvadPFGApgsrMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVR 373
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 647 EHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17652  374 DDRPGDKRLVAYVVPAPGAaPTAAELRAHL----AERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
PRK12316 PRK12316
peptide synthase; Provisional
195-723 2.92e-24

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 109.66  E-value: 2.92e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK12316 2026 DQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLL 2105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  275 TCNAV-KRGPKTINLKaivdaaldqsskdgvsvgiCLTYDnslattrentkwqngRDVWWQDvisqYPTSCEVEWVDAED 353
Cdd:PRK12316 2106 TQRHLlERLPLPAGVA-------------------RLPLD---------------RDAEWAD----YPDTAPAVQLAGEN 2147
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  354 PLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYafdYKSTDvywctADC--------------GWITghsyvtygPMLN 419
Cdd:PRK12316 2148 LAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGER---YELSP-----ADCelqfmsfsfdgaheQWFH--------PLLN 2211
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  420 GATVVVFEGApnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHsRKSLRVLGSVGEPINPSAWRWFFNVVG 499
Cdd:PRK12316 2212 GARVLIRDDE--LWDPEQLYDEMERHGVTILDFPPVYLQQLAEHAE--RDGR-PPAVRVYCFGGEAVPAASLRLAWEALR 2286
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  500 DSRcpISDTWWQTETggfMITPLpgAW---PQKPGSATFPFFGvQPV------IVDEKGNEIEGECSGYLCVKGSwpGAF 570
Cdd:PRK12316 2287 PVY--LFNGYGPTEA---VVTPL--LWkcrPQDPCGAAYVPIG-RALgnrrayILDADLNLLAPGMAGELYLGGE--GLA 2356
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  571 RtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:PRK12316 2357 R---GYLNRPGLTAERfvpdPFSAsgerLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAV 2433
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  643 VVGIEhEVKGQGIYAFVTlleGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEEL 722
Cdd:PRK12316 2434 VVAQD-GASGKQLVAYVV---PDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQLRQA 2509

                  .
gi 378548265  723 G 723
Cdd:PRK12316 2510 Y 2510
PRK08162 PRK08162
acyl-CoA synthetase; Validated
199-724 3.19e-24

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 107.34  E-value: 3.19e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILT--- 275
Cdd:PRK08162  45 TWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFMLRHGEAKVLIVdte 124
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 -CNAVKRGPKTI-NLKAIVDAALDQSSKDGVSVGiCLTYDNSLATTRENTKWQNGRDVWwqDVISqyptscevewvdaed 353
Cdd:PRK08162 125 fAEVAREALALLpGPKPLVIDVDDPEYPGGRFIG-ALDYEAFLASGDPDFAWTLPADEW--DAIA--------------- 186
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 plfLLYTSGSTGKPKGVL-HTTGGYMIYTATTFkyAFDYKSTDVY-----------WCTAdcgW-ITghsyvtygpmLNG 420
Cdd:PRK08162 187 ---LNYTSGTTGNPKGVVyHHRGAYLNALSNIL--AWGMPKHPVYlwtlpmfhcngWCFP---WtVA----------ARA 248
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEGApnypDPGRCWDIVDKYKVSIFYTAPtLVRSLM---RDDDKFVTRHsrkslRVLGSVGEPINPSAwrwffnV 497
Cdd:PRK08162 249 GTNVCLRKV----DPKLIFDLIREHGVTHYCGAP-IVLSALinaPAEWRAGIDH-----PVHAMVAGAAPPAA------V 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 VGdsrcpisdtwwQTETGGFMITPLPG-----------AWpqKPGSATFPFF---------GV----------------Q 541
Cdd:PRK08162 313 IA-----------KMEEIGFDLTHVYGltetygpatvcAW--QPEWDALPLDeraqlkarqGVryplqegvtvldpdtmQ 379
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 542 PVIVDekgNEIEGEC--SGYLCVKGswpgafrtlfgdheryettYFK-------PFAGYYF-SGDGCSRDKDGYYWLTGR 611
Cdd:PRK08162 380 PVPAD---GETIGEImfRGNIVMKG-------------------YLKnpkateeAFAGGWFhTGDLAVLHPDGYIKIKDR 437
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 612 VDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDR 691
Cdd:PRK08162 438 SKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEE---EIIAHCREHLAGFKVPKA 514
                        570       580       590
                 ....*....|....*....|....*....|...
gi 378548265 692 IHWAPgLPKTRSGKIMRRILRKIAsRQLEELGD 724
Cdd:PRK08162 515 VVFGE-LPKTSTGKIQKFVLREQA-KSLKAIDL 545
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
180-645 4.39e-24

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 107.49  E-value: 4.39e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEgnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:COG1022   25 PDRVALREK--EDGIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVPIYPTSSAE 102
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTCNA------VKRGPKTINLKAIVdaALDQssKDGVSVGICLTYDNSLATtrentkwqnGRDVWW 333
Cdd:COG1022  103 EVAYILNDSGAKVLFVEDQeqldklLEVRDELPSLRHIV--VLDP--RGLRDDPRLLSLDELLAL---------GREVAD 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 334 QDVISQYPTScevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVY-----WCtadcgWITG 408
Cdd:COG1022  170 PAELEARRAA-----VKPDDLATIIYTSGTTGRPKGVMLTHRN-LLSNARALLERLPLGPGDRTlsflpLA-----HVFE 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 409 HSyVTYGPMLNGATVVVFEGAPNYPD-------------PgRCW-----DIVDK------YKVSIFYTA----------- 453
Cdd:COG1022  239 RT-VSYYALAAGATVAFAESPDTLAEdlrevkptfmlavP-RVWekvyaGIQAKaeeaggLKRKLFRWAlavgrryarar 316
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 454 ------PTLVRSLMRDDDKFVTRHSRKSL----RVLGSVGEPINPSAWRwFFNVVGdsrCPISDTWWQTETGGFMITPLP 523
Cdd:COG1022  317 lagkspSLLLRLKHALADKLVFSKLREALggrlRFAVSGGAALGPELAR-FFRALG---IPVLEGYGLTETSPVITVNRP 392
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 524 GAwpQKPGSATFPFFGVQpVIVDEKGnEIegecsgylCVKGswPGAFRTLFGDHEryETTyfKPFA--GYYFSGD-GcSR 600
Cdd:COG1022  393 GD--NRIGTVGPPLPGVE-VKIAEDG-EI--------LVRG--PNVMKGYYKNPE--ATA--EAFDadGWLHTGDiG-EL 453
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*.
gi 378548265 601 DKDGYYWLTGRVDDVINVS-GHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:COG1022  454 DEDGFLRITGRKKDLIVTSgGKNVAPQPIENALKASPLIEQAVVVG 499
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
197-720 6.44e-24

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 106.61  E-value: 6.44e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAGFSADSL-----AQRIvdcKPN 271
Cdd:PRK10946  48 QFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--PVNALFSHQRSelnayASQI---EPA 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 272 VILtcnaVKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLATTREntkwQNGRDVWWQdvisqyPTScevewvdA 351
Cdd:PRK10946 123 LLI----ADRQHALFSDDDFLNTLVAEHSSLRVVLLLNDDGEHSLDDAIN----HPAEDFTAT------PSP-------A 181
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHTTGGYmiytattfkyafDY---KSTDVYWCTAD----CGWITGHSYVTYGP-----MLN 419
Cdd:PRK10946 182 DEVAFFQLSGGSTGTPKLIPRTHNDY------------YYsvrRSVEICGFTPQtrylCALPAAHNYPMSSPgalgvFLA 249
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVfegAPNyPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVG 499
Cdd:PRK10946 250 GGTVVL---APD-PSATLCFPLIEKHQVNVTALVPPAVSLWLQAIAEGGSRAQLASLKLLQVGGARLSETLARRIPAELG 325
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dsrCPIS----------------DTWWQT-ETGGFMITPLPGAWpqkpgsatfpffgvqpvIVDEKGNEI-EGEcSGYLC 561
Cdd:PRK10946 326 ---CQLQqvfgmaeglvnytrldDSDERIfTTQGRPMSPDDEVW-----------------VADADGNPLpQGE-VGRLM 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 562 VKGswPGAFRTLFGDHErYETTYFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEA 641
Cdd:PRK10946 385 TRG--PYTFRGYYKSPQ-HNASAFDA-NGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHA 460
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 642 AVVGIEHEVKGQGIYAFVTLLEGVPySEELRKSLvlmvRNQ-IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLE 720
Cdd:PRK10946 461 ALVSMEDELMGEKSCAFLVVKEPLK-AVQLRRFL----REQgIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRAS 535
PRK12467 PRK12467
peptide synthase; Provisional
195-743 7.58e-24

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 108.32  E-value: 7.58e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK12467 3118 DQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLL 3197
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  275 TCNAV-KRGPKTINLKAIVdaaLDqsskdgvsvgicltydnslattrentkwqngRDVWWQdvisqYPTSCEVEWVDAED 353
Cdd:PRK12467 3198 TQAHLlEQLPAPAGDTALT---LD-------------------------------RLDLNG-----YSENNPSTRVMGEN 3238
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  354 PLFLLYTSGSTGKPKGV--------LHTTGGYMIY--TATT-----FKYAFDYKSTDVYWctadcgwitghsyvtygPML 418
Cdd:PRK12467 3239 LAYVIYTSGSTGKPKGVgvrhgalaNHLCWIAEAYelDANDrvllfMSFSFDGAQERFLW-----------------TLI 3301
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  419 NGATVVVfegAPNYP-DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfvtRHSRKSLRVLGSVGEPINPSAW------ 491
Cdd:PRK12467 3302 CGGCLVV---RDNDLwDPEELWQAIHAHRISIACFPPAYLQQFAEDAG----GADCASLDIYVFGGEAVPPAAFeqvkrk 3374
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  492 ---RWFFNVVGDSRCPISDTWWQTETGGfmiTPLPGAWPqkpgsATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpG 568
Cdd:PRK12467 3375 lkpRGLTNGYGPTEAVVTVTLWKCGGDA---VCEAPYAP-----IGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGV--G 3444
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  569 AFRtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAE 640
Cdd:PRK12467 3445 LAR---GYHQRPSLTAERfvadPFSGsggrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVRE 3521
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  641 AAVVGIEHEvKGQGIYAFVTL-LEGVPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQL 719
Cdd:PRK12467 3522 AVVLARDGA-GGKQLVAYVVPaDPQGDWRETLRDHL----AASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDPDAKGS 3596
                         570       580
                  ....*....|....*....|....*.
gi 378548265  720 EELgdtstLADPSVVDQLIAL--ADV 743
Cdd:PRK12467 3597 REY-----VAPRSEVEQQLAAiwADV 3617
ttLC_FACS_like cd05915
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
195-713 1.06e-23

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.


Pssm-ID: 213283 [Multi-domain]  Cd Length: 509  Bit Score: 105.59  E-value: 1.06e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd05915   22 VHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLL 101
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 tcnavkrgpktinlkaiVDAALDQSSKDGVSVgicltydnsLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWVDAEDP 354
Cdd:cd05915  102 -----------------FDPNLLPLVEAIRGE---------LKTVQHFVVMDEKAPEGYLAYEEALGEEADPVRVPERAA 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHT-TGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEgapnYP 433
Cdd:cd05915  156 CGMAYTTGTTGLPKGVVYShRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGP----RL 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 434 DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfVTRHSRKSLRVLGSVGEPinPSAWRWF-----FNVVGDSRCP---- 504
Cdd:cd05915  232 DPASLVELFDGEGVTFTAGVPTVWLALADYLES-TGHRLKTLRRLVVGGSAA--PRSLIARfermgVEVRQGYGLTetsp 308
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 --ISDTW---WQT--ETGGFMITPLPG-------AWPQKPGSATFPFFGVQPVIVDEKGNEIegeCSGYlcvkgswpgaf 570
Cdd:cd05915  309 vvVQNFVkshLESlsEEEKLTLKAKTGlpiplvrLRVADEEGRPVPKDGKALGEVQLKGPWI---TGGY----------- 374
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 rtlFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:cd05915  375 ---YGNEEATRSALTP--DGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPK 449
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 651 KGQGIYAFVTLLEGVPYSEElrksLVLMVRNQIGAFAA-PDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd05915  450 WQERPLAVVVPRGEKPTPEE----LNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
171-721 1.91e-23

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 105.08  E-value: 1.91e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 171 LDKNVeAGLGDKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV-- 248
Cdd:PRK05605  38 YDNAV-ARFGDRPALDFFG------ATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVvv 110
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 249 -HS------------------VVFAGFSADSLAQRIV-DCKPNVILTCNAVKRGP--KTINLKAIVDAALDQSSKDGVSV 306
Cdd:PRK05605 111 eHNplytahelehpfedhgarVAIVWDKVAPTVERLRrTTPLETIVSVNMIAAMPllQRLALRLPIPALRKARAALTGPA 190
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 307 GICLTYDNSLATTRentkwqnGRDvwwqDVISQYPTscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiytattfk 386
Cdd:PRK05605 191 PGTVPWETLVDAAI-------GGD----GSDVSHPR------PTPDDVALILYTSGTTGKPKGAQLTHRNLF-------- 245
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 387 yafdykstdvywCTADCG--WITG---------------HSY-----VTYGPMLnGATVVVFegaPNyPDPGRCWDIVDK 444
Cdd:PRK05605 246 ------------ANAAQGkaWVPGlgdgpervlaalpmfHAYgltlcLTLAVSI-GGELVLL---PA-PDIDLILDAMKK 308
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 445 YKVSIFYTAPTLVRSLMRdddkfVTRHSRKSLrvlgsvgepinpSAWRWFFNvvGDSRCPISDT-WWQTETGGFMI---- 519
Cdd:PRK05605 309 HPPTWLPGVPPLYEKIAE-----AAEERGVDL------------SGVRNAFS--GAMALPVSTVeLWEKLTGGLLVegyg 369
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 520 ----TPLPGAWP----QKPGSATFPFFGVQPVIVD-EKGNEI--EGEcSGYLCVKGswPGAFRtlfGDHERYETTYFKPF 588
Cdd:PRK05605 370 ltetSPIIVGNPmsddRRPGYVGVPFPDTEVRIVDpEDPDETmpDGE-EGELLVRG--PQVFK---GYWNRPEETAKSFL 443
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 589 AGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS 668
Cdd:PRK05605 444 DGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALD 523
                        570       580       590       600       610
                 ....*....|....*....|....*....|....*....|....*....|....
gi 378548265 669 EE-LRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEE 721
Cdd:PRK05605 524 PEgLRAYC----REHLTRYKVPRRFYHVDELPRDQLGKVRRREVREELLEKLGA 573
PRK12316 PRK12316
peptide synthase; Provisional
198-721 3.01e-23

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 106.58  E-value: 3.01e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILT-C 276
Cdd:PRK12316 4577 LTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTqS 4656
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  277 NAVKRGPktinlkaivdaaldqsSKDGVSvgiCLTYDnslattrentkwqngRDVWWQDvisqYPTSCEVEWVDAEDPLF 356
Cdd:PRK12316 4657 HLLQRLP----------------IPDGLA---SLALD---------------RDEDWEG----FPAHDPAVRLHPDNLAY 4698
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  357 LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYafdykstdvYWCTADCGWITGHSYV-------TYGPMLNGATVVVfeGA 429
Cdd:PRK12316 4699 VIYTSGSTGRPKGVAVSHGSLVNHLHATGER---------YELTPDDRVLQFMSFSfdgshegLYHPLINGASVVI--RD 4767
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  430 PNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtRHSRKSLRVLGSVGEPINPS----AWR-----WFFNVVGD 500
Cdd:PRK12316 4768 DSLWDPERLYAEIHEHRVTVLVFPPVYLQQLAEHAER---DGEPPSLRVYCFGGEAVAQAsydlAWRalkpvYLFNGYGP 4844
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  501 SRCPISDTWWQTETGgfmitplpgawpQKPGSATFPFFGVQP----VIVDEKGNEIEGECSG--YLCVKGSWPGAFRTLF 574
Cdd:PRK12316 4845 TETTVTVLLWKARDG------------DACGAAYMPIGTPLGnrsgYVLDGQLNPLPVGVAGelYLGGEGVARGYLERPA 4912
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  575 GDHERYETTYF-KPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVkGQ 653
Cdd:PRK12316 4913 LTAERFVPDPFgAPGGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAV-GK 4991
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265  654 GIYAFVT-----LLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEE 721
Cdd:PRK12316 4992 QLVGYVVpqdpaLADADEAQAELRDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKALPQPDASLLQQ 5064
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
197-715 8.44e-23

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 102.41  E-value: 8.44e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNgVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAGFSADSLAQR--IVDCKPNVIL 274
Cdd:cd05909    7 SLTYRKLLTGAIALARKLAKM-TKEGENVGVMLPPSAGGALANFALALSGKV--PVMLNYTAGLRELRacIKLAGIKTVL 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCnavKRGPKTINLKAIVDAALDQ------------SSKDGVSVGICLTYdnslattrentkwqngRDVWWqdvISQYPT 342
Cdd:cd05909   84 TS---KQFIEKLKLHHLFDVEYDArivyledlrakiSKADKCKAFLAGKF----------------PPKWL---LRIFGV 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 343 SCevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYM--IYTATTFkyaFDYKSTDVYwctadCGWITG-HSY----VTYG 415
Cdd:cd05909  142 AP----VQPDDPAVILFTSGSEGLPKGVVLSHKNLLanVEQITAI---FDPNPEDVV-----FGALPFfHSFgltgCLWL 209
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 416 PMLNGATVVVfegAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRdddkFVTRHSRKSLRVLGSVGEPINPSAWRWFF 495
Cdd:cd05909  210 PLLSGIKVVF---HPNPLDYKKIPELIYDKKATILLGTPTFLRGYAR----AAHPEDFSSLRLVVAGAEKLKDTLRQEFQ 282
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 496 NVVGdsrCPISDTWWQTETGGFMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNE--IEGEcSGYLCVKGswPGAFRTL 573
Cdd:cd05909  283 EKFG---IRILEGYGTTECSPVISVNTPQS-PNKEGTVGRPLPGMEVKIVSVETHEevPIGE-GGLLLVRG--PNVMLGY 355
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FGDHERyetTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL-VLHPQCAEAAVVGIEHEVKG 652
Cdd:cd05909  356 LNEPEL---TSFAFGDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILsEILPEDNEVAVVSVPDGRKG 432
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 653 QGIYAFVTLLEGVPyseelrKSLVLMVRN-QIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:cd05909  433 EKIVLLTTTTDTDP------SSLNDILKNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTLKALA 490
FACL_like_5 cd05924
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
352-705 9.22e-23

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341248 [Multi-domain]  Cd Length: 364  Bit Score: 100.53  E-value: 9.22e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHTTG--GYMIYTATTFKYAFDYKSTDVYWCTAD---------CGWITGHSYVTYGPMLNG 420
Cdd:cd05924    3 ADDLYILYTGGTTGMPKGVMWRQEdiFRMLMGGADFGTGEFTPSEDAHKAAAAaagtvmfpaPPLMHGTGSWTAFGGLLG 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEGaPNYpDPGRCWDIVDKYKV-SIFYTAPTLVRSLMrddDKFVTRHSR--KSLRVLGSVGEPINPSAWRWFFNV 497
Cdd:cd05924   83 GQTVVLPD-DRF-DPEEVWRTIEKHKVtSMTIVGDAMARPLI---DALRDAGPYdlSSLFAISSGGALLSPEVKQGLLEL 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 VGDSRcpISDTWWQTETGgFMITPLPGawPQKPGSATFPFFGVQPVIVDEKGNEIE--GECSGYLCVKGSWPGAFrtlFG 575
Cdd:cd05924  158 VPNIT--LVDAFGSSETG-FTGSGHSA--GSGPETGPFTRANPDTVVLDDDGRVVPpgSGGVGWIARRGHIPLGY---YG 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 DHERYETTyFKPFAG--YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:cd05924  230 DEAKTAET-FPEVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQ 308
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|..
gi 378548265 654 GIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGK 705
Cdd:cd05924  309 EVVAVVQLREGAGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
353-708 9.78e-23

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 100.04  E-value: 9.78e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVV--Fegap 430
Cdd:cd17637    1 DPFVIIHTAAVAGRPRGAVLSHGN-LIAANLQLIHAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMekF---- 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 431 nypDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRwffnvvgdSRCPisDTWW 510
Cdd:cd17637   76 ---DPAEALELIEEEKVTLMGSFPPILSNLLDAAEK--SGVDLSSLRHVLGLDAPETIQRFE--------ETTG--ATFW 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 511 ----QTETGGFmITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTLFGDHERYETTYFK 586
Cdd:cd17637  141 slygQTETSGL-VTLSPYR--ERPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRG--PLVFQGYWNLPELTAYTFRN 215
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 587 pfaGYYFSGDGCSRDKDGYYWLTGRV--DDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEG 664
Cdd:cd17637  216 ---GWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPG 292
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 378548265 665 VPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17637  293 ATLTAD---ELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
199-713 1.53e-22

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 102.14  E-value: 1.53e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVviylpmlmelpiAMLA--CAR----------IGAVHSVVFAGFSADSLAQRIV 266
Cdd:PRK06018  41 TYAQIHDRALKVSQALDRDGIKLGDRV------------ATIAwnTWRhleawygimgIGAICHTVNPRLFPEQIAWIIN 108
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 267 DCKPNVILTcnavkrgpkTINLKAIVDAALDQSSKdgVSVGICLTYDNSL-ATTRENTkwqngrdVWWQDVISQYPTSCE 345
Cdd:PRK06018 109 HAEDRVVIT---------DLTFVPILEKIADKLPS--VERYVVLTDAAHMpQTTLKNA-------VAYEEWIAEADGDFA 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 346 VEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYT-ATTFKYAFDYKSTDVY-----------WCTADCGWITGHSYVT 413
Cdd:PRK06018 171 WKTFDENTAAGMCYTSGTTGDPKGVLYSHRSNVLHAlMANNGDALGTSAADTMlpvvplfhansWGIAFSAPSMGTKLVM 250
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLNGATVvvfegapnypdpgrcWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhSRKSLRVLGSVgepinpsawrw 493
Cdd:PRK06018 251 PGAKLDGASV---------------YELLDTEKVTFTAGVPTVWLMLLQYMEK-----EGLKLPHLKMV----------- 299
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 494 ffnVVGDSRCPIS-------------DTWWQTETGGF-MITPLPGAWPQKPGSAT--------FPFFGVQPVIVDEKGNE 551
Cdd:PRK06018 300 ---VCGGSAMPRSmikafedmgvevrHAWGMTEMSPLgTLAALKPPFSKLPGDARldvlqkqgYPPFGVEMKITDDAGKE 376
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 552 I--EGECSGYLCVKGswPGAFRTLFgdheRYETTYFKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVE 629
Cdd:PRK06018 377 LpwDGKTFGRLKVRG--PAVAAAYY----RVDGEILDD-DGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLE 449
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 630 SALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:PRK06018 450 NLAVGHPKVAEAAVIGVYHPKWDERPLLIVQLKPGEtATREEILKYM----DGKIAKWWMPDDVAFVDAIPHTATGKILK 525

                 ....*
gi 378548265 709 RILRK 713
Cdd:PRK06018 526 TALRE 530
PRK12316 PRK12316
peptide synthase; Provisional
198-711 1.81e-22

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 103.88  E-value: 1.81e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCN 277
Cdd:PRK12316  537 LDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLSQS 616
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  278 AVKRgpktinlKAIVDAALDQsskdgvsvgicLTYDNSlattrenTKWQNGrdvwwqdvisqYPTSCEVEWVDAEDPLFL 357
Cdd:PRK12316  617 HLGR-------KLPLAAGVQV-----------LDLDRP-------AAWLEG-----------YSEENPGTELNPENLAYV 660
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  358 LYTSGSTGKPKGVLHTTGG---YMIYTATTFK------------YAFDYKSTDVYWctadcgwitghsyvtygPMLNGAT 422
Cdd:PRK12316  661 IYTSGSTGKPKGAGNRHRAlsnRLCWMQQAYGlgvgdtvlqktpFSFDVSVWEFFW-----------------PLMSGAR 723
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  423 VVVfEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrHSRKSLRVLGSVGEPINPSAW------RW--- 493
Cdd:PRK12316  724 LVV-AAPGDHRDPAKLVELINREGVDTLHFVPSMLQAFLQDEDV----ASCTSLRRIVCSGEALPADAQeqvfakLPqag 798
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  494 FFNVVGDSRCPISDTWWQTETGGFMITPLpGAwpqkpgsatfPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRTL 573
Cdd:PRK12316  799 LYNLYGPTEAAIDVTHWTCVEEGGDSVPI-GR----------PIANLACYILDANLEPVPVGVLGELYLAGR--GLARGY 865
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  574 FG----DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEhe 649
Cdd:PRK12316  866 HGrpglTAERFVPSPFVAGERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVD-- 943
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265  650 vkGQGIYAFVtllegVPYSE--ELRKSLVLMVRNQIGAFAAPDriHWAP--GLPKTRSGKIMRRIL 711
Cdd:PRK12316  944 --GKQLVGYV-----VLESEggDWREALKAHLAASLPEYMVPA--QWLAleRLPLTPNGKLDRKAL 1000
PRK07787 PRK07787
acyl-CoA synthetase; Validated
527-712 2.06e-22

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 101.22  E-value: 2.06e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 527 PQKPGSATFPFFGVQPVIVDEKGNEI--EGECSGYLCVKGSwpgafrTLF-GDHERYETT--YFKPfAGYYFSGDGCSRD 601
Cdd:PRK07787 289 ERRPGWVGLPLAGVETRLVDEDGGPVphDGETVGELQVRGP------TLFdGYLNRPDATaaAFTA-DGWFRTGDVAVVD 361
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 602 KDGYYWLTGRVD-DVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEelrksLVLMVR 680
Cdd:PRK07787 362 PDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVAADE-----LIDFVA 436
                        170       180       190
                 ....*....|....*....|....*....|..
gi 378548265 681 NQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK07787 437 QQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
197-645 3.74e-22

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 100.36  E-value: 3.74e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIltc 276
Cdd:cd05907    5 PITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKAL--- 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navkrgpktinlkaIVDaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvDAEDPLF 356
Cdd:cd05907   82 --------------FVE--------------------------------------------------------DPDDLAT 91
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDpg 436
Cdd:cd05907   92 IIYTSGTTGRPKGVMLSHRNIL-SNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYFASSAETLLD-- 168
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 437 rcwDIVdKYKVSIFYTAPTL---VRSLMRDDD------KFVTRHSRKSLRVLGSVGEPINPSAWRwFFNVVGdsrCPISD 507
Cdd:cd05907  169 ---DLS-EVRPTVFLAVPRVwekVYAAIKVKAvpglkrKLFDLAVGGRLRFAASGGAPLPAELLH-FFRALG---IPVYE 240
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 508 TWWQTETGGFMITPLPGAWpqKPGSATFPFFGVQPVIVDekgneiEGEcsgyLCVKGswPGAFRTLFGDHEryETTYFKP 587
Cdd:cd05907  241 GYGLTETSAVVTLNPPGDN--RIGTVGKPLPGVEVRIAD------DGE----ILVRG--PNVMLGYYKNPE--ATAEALD 304
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 378548265 588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVI-NVSGHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:cd05907  305 ADGWLHTGDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLISQAVVIG 363
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
177-713 5.82e-22

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 100.39  E-value: 5.82e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 177 AGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGvKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFA-- 254
Cdd:cd05931    4 AARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPpt 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 255 -GFSADSLAQRIVDCKPNVILTCNAVKRGpktinlkaiVDAALDQSSKDGVSVGICLTydnSLATTRENTkwqngrdvwW 333
Cdd:cd05931   83 pGRHAERLAAILADAGPRVVLTTAAALAA---------VRAFAASRPAAGTPRLLVVD---LLPDTSAAD---------W 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 334 QDVIsqyptscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFK-YAFDYKSTDVYW--CTADCGWITGhs 410
Cdd:cd05931  142 PPPS-----------PDPDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRRaYGLDPGDVVVSWlpLYHDMGLIGG-- 208
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 yvTYGPMLNGATVVVFegAPNY--PDPGRcW-DIVDKYKVSIFYtAPT----LVRSLMRDDDKFVTRHSrkSLRVLGSVG 483
Cdd:cd05931  209 --LLTPLYSGGPSVLM--SPAAflRRPLR-WlRLISRYRATISA-APNfaydLCVRRVRDEDLEGLDLS--SWRVALNGA 280
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EPINPSAWRWFFN--------------------------VVGDSRCPISDTWWQTETGGfmitPLPGAWPQKPGSATF-- 535
Cdd:cd05931  281 EPVRPATLRRFAEafapfgfrpeafrpsyglaeatlfvsGGPPGTGPVVLRVDRDALAG----RAVAVAADDPAARELvs 356
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 536 ---PFFGVQPVIVDEKGNE-----IEGE--------CSGYLCVKGSWPGAFRTLFGDHERyettyfkpfaGYYFSGD-GC 598
Cdd:cd05931  357 cgrPLPDQEVRIVDPETGRelpdgEVGEiwvrgpsvASGYWGRPEATAETFGALAATDEG----------GWLRTGDlGF 426
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 599 SRDkdGYYWLTGRVDDVINVSGHRIGTAEVESAL-----VLHPQCAEAAVVGIEHEVKGqGIYAFVTLLEGVPYSEELRK 673
Cdd:cd05931  427 LHD--GELYITGRLKDLIIVRGRNHYPQDIEATAeeahpALRPGCVAAFSVPDDGEERL-VVVAEVERGADPADLAAIAA 503
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|..
gi 378548265 674 SLVLMVRNQIGafAAPDRIHWAP--GLPKTRSGKIMRRILRK 713
Cdd:cd05931  504 AIRAAVAREHG--VAPADVVLVRpgSIPRTSSGKIQRRACRA 543
PRK12316 PRK12316
peptide synthase; Provisional
195-743 8.09e-22

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 101.96  E-value: 8.09e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK12316 3080 EQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLL 3159
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  275 TcnavkrgpktinlkaivDAALDQSSKDGVSVgICLTYDNSlattrentkwqngrdvwwqdvisQYPTSCEVEWVDAEDP 354
Cdd:PRK12316 3160 S-----------------QSHLRLPLAQGVQV-LDLDRGDE-----------------------NYAEANPAIRTMPENL 3198
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  355 LFLLYTSGSTGKPKGVLHTTGGYMIYT-ATTFKYAFDYKSTDVYWCTADcgwITGHSYVTYGPMLNGATVVVfEGAPNYP 433
Cdd:PRK12316 3199 AYVIYTSGSTGKPKGVGIRHSALSNHLcWMQQAYGLGVGDRVLQFTTFS---FDVFVEELFWPLMSGARVVL-AGPEDWR 3274
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  434 DPGRCWDIVDKYKVSIfytaPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPS-AWRWF-----FNVVGDSRCPISD 507
Cdd:PRK12316 3275 DPALLVELINSEGVDV----LHAYPSMLQAFLEEEDAHRCTSLKRIVCGGEALPADlQQQVFaglplYNLYGPTEATITV 3350
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  508 TWWQTETGGFMITPLPGAWPQKpgSATFPFFGVQPVIVDEKGNEiegecsgYLCVKGSWPGAFRTLFGDHERYETTYFKP 587
Cdd:PRK12316 3351 THWQCVEEGKDAVPIGRPIANR--ACYILDGSLEPVPVGALGEL-------YLGGEGLARGYHNRPGLTAERFVPDPFVP 3421
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  588 FAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVgiehEVKGQGIYAFVTLLEGVPy 667
Cdd:PRK12316 3422 GERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVL----AVDGRQLVAYVVPEDEAG- 3496
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265  668 seELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEelgdTSTLADPSVVDQLIA--LADV 743
Cdd:PRK12316 3497 --DLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPDAALLQ----QDYVAPVNELERRLAaiWADV 3568
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
177-723 8.46e-22

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 99.85  E-value: 8.46e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 177 AGLGDKTAIHWEGNELGV----DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV---- 248
Cdd:PRK12583  21 GDAFDATVARFPDREALVvrhqALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAIlvni 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 249 ------------------HSVVFA-GFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAIVdaALDQSSKDGVsvgic 309
Cdd:PRK12583 101 npayraseleyalgqsgvRWVICAdAFKTSDYHAMLQELLPGLAEGQPGALACERLPELRGVV--SLAPAPPPGF----- 173
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 310 LTYDNSLATtrentkwqnGRDVWWQDVISQYPTscevewVDAEDPLFLLYTSGSTGKPKGVLHT-----TGGYMIYTATT 384
Cdd:PRK12583 174 LAWHELQAR---------GETVSREALAERQAS------LDRDDPINIQYTSGTTGFPKGATLShhnilNNGYFVAESLG 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 385 FKyafdykSTDV-------YWCTadcgwitGHSYVTYGPMLNGATVVVfegaPN-YPDPGRCWDIVDKYKVSIFYTAPTL 456
Cdd:PRK12583 239 LT------EHDRlcvpvplYHCF-------GMVLANLGCMTVGACLVY----PNeAFDPLATLQAVEEERCTALYGVPTM 301
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 457 VRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRwffNVVGDSRCP-ISDTWWQTETGGfmITPLPGAWPQKPGSATf 535
Cdd:PRK12583 302 FIAELDHPQR--GNFDLSSLRTGIMAGAPCPIEVMR---RVMDEMHMAeVQIAYGMTETSP--VSLQTTAADDLERRVE- 373
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 536 PFFGVQP----VIVDEKGNEI----EGE-CS-GYLCVKGSWPGAFRTLFGDHERyettyfkpfaGYYFSGDGCSRDKDGY 605
Cdd:PRK12583 374 TVGRTQPhlevKVVDPDGATVprgeIGElCTrGYSVMKGYWNNPEATAESIDED----------GWMHTGDLATMDEQGY 443
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 606 YWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKslvlMVRNQIG 684
Cdd:PRK12583 444 VRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASeEELRE----FCKARIA 519
                        570       580       590
                 ....*....|....*....|....*....|....*....
gi 378548265 685 AFAAPDRIHWAPGLPKTRSGKIMRRILRKIAsrqLEELG 723
Cdd:PRK12583 520 HFKVPRYFRFVDEFPMTVTGKVQKFRMREIS---IEELA 555
PRK12467 PRK12467
peptide synthase; Provisional
198-739 1.43e-21

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 101.01  E-value: 1.43e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCN 277
Cdd:PRK12467 1600 LTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQS 1679
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  278 AV-KRGPKTINLKAIVdaaLDQSskdgvsvgicltydnslattrentkwqngrdvwwQDVISQYPTSCEVEWVDAEDPLF 356
Cdd:PRK12467 1680 HLqARLPLPDGLRSLV---LDQE----------------------------------DDWLEGYSDSNPAVNLAPQNLAY 1722
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  357 LLYTSGSTGKPKGVLHTTGGYM-IYTATTFKYAFdykstdvywCTADCgWITGHSYV-------TYGPMLNGATVVVfeG 428
Cdd:PRK12467 1723 VIYTSGSTGRPKGAGNRHGALVnRLCATQEAYQL---------SAADV-VLQFTSFAfdvsvweLFWPLINGARLVI--A 1790
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  429 APN-YPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVtrHSRkSLRVLGSVGEPINPSAWR-WF--------FNVV 498
Cdd:PRK12467 1791 PPGaHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVE--HPL-SLRRVVCGGEALEVEALRpWLerlpdtglFNLY 1867
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  499 GDSRCPISDTWWQTETGgfmitPLPGAWPQKPGSatfPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRtlfGDHE 578
Cdd:PRK12467 1868 GPTETAVDVTHWTCRRK-----DLEGRDSVPIGQ---PIANLSTYILDASLNPVPIGVAGELYLGGV--GLAR---GYLN 1934
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  579 RYETTYFK----PFAGY----YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:PRK12467 1935 RPALTAERfvadPFGTVgsrlYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQDGAN 2014
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  651 KGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAfAAPDRI---HWA--PGLPKTRSGKIMRRILRKIASRQLEelgdT 725
Cdd:PRK12467 2015 GKQLVAYVVPTDPGLVDDDEAQVALRAILKNHLKA-SLPEYMvpaHLVflARMPLTPNGKLDRKALPAPDASELQ----Q 2089
                         570
                  ....*....|....
gi 378548265  726 STLADPSVVDQLIA 739
Cdd:PRK12467 2090 AYVAPQSELEQRLA 2103
PLN02246 PLN02246
4-coumarate--CoA ligase
196-713 2.04e-21

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 98.51  E-value: 2.04e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 196 ASLTYSE--LLQRvcQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVI 273
Cdd:PLN02246  49 RVYTYADveLLSR--RVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKASGAKLI 126
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 274 LTcnavkrgpktinLKAIVDAALDQSSKDGVSVgICL--TYDNSLATTrentkwqngrdvwwqDVISQYPTSCEVEWVDA 351
Cdd:PLN02246 127 IT------------QSCYVDKLKGLAEDDGVTV-VTIddPPEGCLHFS---------------ELTQADENELPEVEISP 178
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHTTGGYMiytaTTFKYAFDYKSTDVYWCTAD---CGWITGHSYVTYGPMLN----GATVV 424
Cdd:PLN02246 179 DDVVALPYSSGTTGLPKGVMLTHKGLV----TSVAQQVDGENPNLYFHSDDvilCVLPMFHIYSLNSVLLCglrvGAAIL 254
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 425 VFegaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfVTRHSRKSLRVLGSVGEPINPS---AWRwffnvvgdS 501
Cdd:PLN02246 255 IM---PKF-EIGALLELIQRHKVTIAPFVPPIVLAIAKSPV--VEKYDLSSIRMVLSGAAPLGKEledAFR--------A 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 502 RCP---ISDTWWQTETG-------GFMITPLPgawpQKPGSATFPFFGVQPVIVD-EKGNEIEGECSGYLCVKGswPGAF 570
Cdd:PLN02246 321 KLPnavLGQGYGMTEAGpvlamclAFAKEPFP----VKSGSCGTVVRNAELKIVDpETGASLPRNQPGEICIRG--PQIM 394
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 RTLFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:PLN02246 395 KGYLNDPEATANTIDK--DGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPSIADAAVVPMKDEV 472
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 651 KGQGIYAFVTLLEGVPYSEELRKSLvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PLN02246 473 AGEVPVAFVVRSNGSEITEDEIKQF---VAKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLRA 532
PRK07514 PRK07514
malonyl-CoA synthase; Validated
181-712 3.99e-21

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 97.64  E-value: 3.99e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIhwegnELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK07514  17 DAPFI-----ETPDGLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAE 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILtCNAVKRGPktinLKAIVDAAldqsskdgvSVGICLTYD----NSLAttrentkwqngrdvwwqDV 336
Cdd:PRK07514  92 LDYFIGDAEPALVV-CDPANFAW----LSKIAAAA---------GAPHVETLDadgtGSLL-----------------EA 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWctadcgwitgHS------ 410
Cdd:PRK07514 141 AAAAPDDFETVPRGADDLAAILYTSGTTGRSKGAMLSHGN-LLSNALTLVDYWRFTPDDVLI----------HAlpifht 209
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 ---YV-TYGPMLNGATVVVFegaPNYpDPGRcwdIVDKY-KVSIFYTAPTLVRSLMRDD--DKFVTRHSRksLRVLGSVg 483
Cdd:PRK07514 210 hglFVaTNVALLAGASMIFL---PKF-DPDA---VLALMpRATVMMGVPTFYTRLLQEPrlTREAAAHMR--LFISGSA- 279
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 ePINPSAWRWFFNVVGDsrcPISDTWWQTETGgfMIT--PLPGAwpQKPGSATFPFFGVQPVIVD-EKGNEIEGECSGYL 560
Cdd:PRK07514 280 -PLLAETHREFQERTGH---AILERYGMTETN--MNTsnPYDGE--RRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMI 351
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 561 CVKGswPGAFRtlfGDHERYETTY--FKPfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQC 638
Cdd:PRK07514 352 EVKG--PNVFK---GYWRMPEKTAeeFRA-DGFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGV 425
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 639 AEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK07514 426 VESAVIGVPHPDFGEGVTAVVVPKPGAALDEA---AILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLR 496
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
193-713 5.25e-21

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 96.61  E-value: 5.25e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 193 GVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVfagfSADSLAQRIvdckpnv 272
Cdd:cd17653   18 SLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPL----DAKLPSARI------- 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 iltcnavkrgpktinlkaivDAALDQSSKDgvsvgICLTYDNslattrentkwqngrdvwwqdvisqyptscevewvdAE 352
Cdd:cd17653   87 --------------------QAILRTSGAT-----LLLTTDS------------------------------------PD 105
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGV-----------------LHTTGGYMIytATTFKYAFDYkstdvywCTAdcgwitghsyVTYG 415
Cdd:cd17653  106 DLAYIIFTSGSTGIPKGVmvphrgvlnyvsqpparLDVGPGSRV--AQVLSIAFDA-------CIG----------EIFS 166
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 416 PMLNGATVVVFEGAPNYPDPGRcwdivdkyKVSIFYTAPTLVRSLMRDDdkfvtrhsRKSLRVLGSVGEPINPS---AW- 491
Cdd:cd17653  167 TLCNGGTLVLADPSDPFAHVAR--------TVDALMSTPSILSTLSPQD--------FPNLKTIFLGGEAVPPSlldRWs 230
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 492 --RWFFNVVGDSRCPISDTWWQTETGGFMI--TPLPGAwpqkpgsatfpffGVqpVIVDEKGNEIEGECSGYLCVKGswP 567
Cdd:cd17653  231 pgRRLYNAYGPTECTISSTMTELLPGQPVTigKPIPNS-------------TC--YILDADLQPVPEGVVGEICISG--V 293
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 GAFRTLFGDHERyETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:cd17653  294 QVARGYLGNPAL-TASKFVPDPFWpgsrmYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTQA 372
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 643 VVgIEHEvkgQGIYAFVTllegvPYS---EELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:cd17653  373 AA-IVVN---GRLVAFVT-----PETvdvDGLRSEL----AKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
352-712 1.11e-20

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 94.27  E-value: 1.11e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHT-----TGGYMIytattfKYAFDYKSTDV-------YWCTadcGWITGH-SYVTYGpml 418
Cdd:cd05917    2 DDVINIQFTSGTTGSPKGATLThhnivNNGYFI------GERLGLTEQDRlcipvplFHCF---GSVLGVlACLTHG--- 69
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 ngATVVVfeGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvTRHSRKSLR--VLGsvGEPINPSAWRWFFN 496
Cdd:cd05917   70 --ATMVF--PSPSF-DPLAVLEAIEKEKCTALHGVPTMFIAELEHPDF--DKFDLSSLRtgIMA--GAPCPPELMKRVIE 140
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 VVGDSRCPISdtWWQTET--GGFMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGNE-----IEGE--CSGYLCVKGSWp 567
Cdd:cd05917  141 VMNMKDVTIA--YGMTETspVSTQTRTDDSI-EKRVNTVGRIMPHTEAKIVDPEGGIvppvgVPGElcIRGYSVMKGYW- 216
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 gafrtlfGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:cd05917  217 -------NDPEKTAEAIDG--DGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVP 287
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEE-LRKslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR 712
Cdd:cd05917  288 DERYGEEVCAWIRLKEGAELTEEdIKA----YCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
196-712 6.30e-20

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 94.35  E-value: 6.30e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 196 ASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAV--------------HSVVFAGFSA-- 258
Cdd:PRK08974  47 EVMTFRKLEERSRAFAAYLQNGlGLKKGDRVALMMPNLLQYPIALFGILRAGMIvvnvnplytpreleHQLNDSGAKAiv 126
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 -----DSLAQRIVDCKP--NVILT--CNAVKRGPKTInlkaiVDAALDQSSKdgvsvgICLTYDNSLATTRENTkWQNGR 329
Cdd:PRK08974 127 ivsnfAHTLEKVVFKTPvkHVILTrmGDQLSTAKGTL-----VNFVVKYIKR------LVPKYHLPDAISFRSA-LHKGR 194
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 330 DVwwqdvisQY--PTscevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIytattfkyafdyksTDVYWCTAdcgwit 407
Cdd:PRK08974 195 RM-------QYvkPE------LVPEDLAFLQYTGGTTGVAKGAMLTHRN-ML--------------ANLEQAKA------ 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 408 ghsyvTYGPMLNGATVVVFEGAPNY---PDPGRCWDIVDKYKVSIFYTAPtlvrslmRDDDKFVTRHSRKSLRVLGSVGE 484
Cdd:PRK08974 241 -----AYGPLLHPGKELVVTALPLYhifALTVNCLLFIELGGQNLLITNP-------RDIPGFVKELKKYPFTAITGVNT 308
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 485 PINPSAWRWFFNVVGDSRCPIS-----------DTWWQTETG-----GFMIT---PLPGAWP----QKPGSATFPFFGVQ 541
Cdd:PRK08974 309 LFNALLNNEEFQELDFSSLKLSvgggmavqqavAERWVKLTGqylleGYGLTecsPLVSVNPydldYYSGSIGLPVPSTE 388
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 542 PVIVDEKGNEIEGECSGYLCVKGSwpgafRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGH 621
Cdd:PRK08974 389 IKLVDDDGNEVPPGEPGELWVKGP-----QVMLGYWQRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKKDMILVSGF 463
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 622 RIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSlvlmVRNQIGAFAAPDRIHWAPGLPKT 701
Cdd:PRK08974 464 NVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVVKKDPSLTEEELITH----CRRHLTGYKVPKLVEFRDELPKS 539
                        570
                 ....*....|.
gi 378548265 702 RSGKIMRRILR 712
Cdd:PRK08974 540 NVGKILRRELR 550
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
198-728 6.31e-20

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 94.10  E-value: 6.31e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVI-------YLPMlmelpiaMLACARIGAVHSVVFAGFSADSLAQRIVDCKP 270
Cdd:PLN02860  33 RTGHEFVDGVLSLAAGLLRLGLRNGDVVAIaalnsdlYLEW-------LLAVACAGGIVAPLNYRWSFEEAKSAMLLVRP 105
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 271 NVILTCNAVKRGPKTINLKAI----VDAALDQSSKDGVSVgicltyDNSLATTRENTKWQNGrdvwwqdvisqyPTSCEV 346
Cdd:PLN02860 106 VMLVTDETCSSWYEELQNDRLpslmWQVFLESPSSSVFIF------LNSFLTTEMLKQRALG------------TTELDY 167
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 347 EWVdAEDPLFLLYTSGSTGKPKGVL--HTTggymIYTATTFKYAF-DYKSTDVYWCTADCGWITGHSYVTYGPMLNGATV 423
Cdd:PLN02860 168 AWA-PDDAVLICFTSGTTGRPKGVTisHSA----LIVQSLAKIAIvGYGEDDVYLHTAPLCHIGGLSSALAMLMVGACHV 242
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVfegaPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVL----GSVGEPINPSAWRWFfnvvg 499
Cdd:PLN02860 243 LL----PKF-DAKAALQAIKQHNVTSMITVPAMMADLISLTRKSMTWKVFPSVRKIlnggGSLSSRLLPDAKKLF----- 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dSRCPISDTWWQTETGG---FMitPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRT--LF 574
Cdd:PLN02860 313 -PNAKLFSAYGMTEACSsltFM--TLHDPTLESPKQTLQTVNQTKSSSVHQPQGVCVGKPAPHVELKIGLDESSRVgrIL 389
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 575 --GDH--ERY-----ETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVG 645
Cdd:PLN02860 390 trGPHvmLGYwgqnsETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVG 469
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 646 IEHEVKGQGIYAFVTLLEGVPYS----EELRKSLVL-------MVRNQ-IGAFAAPDRI-HWAPGLPKTRSGKIMRRILR 712
Cdd:PLN02860 470 VPDSRLTEMVVACVRLRDGWIWSdnekENAKKNLTLssetlrhHCREKnLSRFKIPKLFvQWRKPFPLTTTGKIRRDEVR 549
                        570
                 ....*....|....*.
gi 378548265 713 KIASRQLEELgdTSTL 728
Cdd:PLN02860 550 REVLSHLQSL--PSNL 563
PRK05691 PRK05691
peptide synthase; Validated
181-711 6.62e-20

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 95.62  E-value: 6.62e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  181 DKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK05691 1146 ERIALVWDG------GSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPDYPAER 1219
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  261 LAQRIVDCKPNVILTCNAV-KRGPKTINLKAIvdaALDQsskdgvsvgicLTYDNslattrentkwqngrdvwwqdvisq 339
Cdd:PRK05691 1220 LAYMLADSGVELLLTQSHLlERLPQAEGVSAI---ALDS-----------LHLDS------------------------- 1260
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  340 YPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGG------YMIYTattfkYAFDykSTDVYWCTA---------DCG 404
Cdd:PRK05691 1261 WPSQAPGLHLHGDNLAYVIYTSGSTGQPKGVGNTHAAlaerlqWMQAT-----YALD--DSDVLMQKApisfdvsvwECF 1333
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  405 WitghsyvtygPMLNGATVVVfEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRhsrkSLRVLGSVGE 484
Cdd:PRK05691 1334 W----------PLITGCRLVL-AGPGEHRDPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAAACT----SLRRLFSGGE 1398
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  485 PInPSAWR----------WFFNVVGDSRCPISDTWWQTETGGFMITPLpgawpqkpGSatfPFFGVQPVIVDEKGNEIEG 554
Cdd:PRK05691 1399 AL-PAELRnrvlqrlpqvQLHNRYGPTETAINVTHWQCQAEDGERSPI--------GR---PLGNVLCRVLDAELNLLPP 1466
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  555 ECSGYLCVKGSwpGAFRTLFG----DHERY-ETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVE 629
Cdd:PRK05691 1467 GVAGELCIGGA--GLARGYLGrpalTAERFvPDPLGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQ 1544
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  630 SALVLHPQCAEAAVVgIEHEVKGQGIYAFVTLLEGV-PYSEELRKSLVL-----MVRNQIGAFAApdrihwapgLPKTRS 703
Cdd:PRK05691 1545 ARLLAQPGVAQAAVL-VREGAAGAQLVGYYTGEAGQeAEAERLKAALAAelpeyMVPAQLIRLDQ---------MPLGPS 1614

                  ....*...
gi 378548265  704 GKIMRRIL 711
Cdd:PRK05691 1615 GKLDRRAL 1622
ACAS_N pfam16177
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many ...
110-170 7.17e-20

Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many acetyl-coenzyme A synthetase enzymes.


Pssm-ID: 465043 [Multi-domain]  Cd Length: 55  Bit Score: 83.29  E-value: 7.17e-20
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 378548265  110 YMEMHKRSMDDPAAFWSDIASEFYWKQKWgDQVfsenLDVRKGPiSIEWFKGGITNICYNC 170
Cdd:pfam16177   1 YEALYRRSIEDPEGFWGEVAKELDWFKPF-DKV----LDGSNGP-FAKWFVGGKLNVCYNC 55
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
352-708 2.21e-19

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 90.01  E-value: 2.21e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 352 EDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGhsyvtygpMLNGATVVVFEGA-- 429
Cdd:cd17635    1 EDPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGG--------LWWILTCLIHGGLcv 72
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 430 --PNYPDPGRCWDIVDKYKVSIFYTAPTL---VRSLMRDDDKFVtrhsrKSLRVLGSVGE-PINpsAWRWFFNVVGDSRc 503
Cdd:cd17635   73 tgGENTTYKSLFKILTTNAVTTTCLVPTLlskLVSELKSANATV-----PSLRLIGYGGSrAIA--ADVRFIEATGLTN- 144
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 pISDTWWQTETGGFMITPLpGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWpgafrTLFGDHERYETT 583
Cdd:cd17635  145 -TAQVYGLSETGTALCLPT-DDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPA-----NMLGYWNNPERT 217
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 584 YFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLle 663
Cdd:cd17635  218 AEVLIDGWVNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVA-- 295
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*....
gi 378548265 664 gvpySEELRKS----LVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17635  296 ----SAELDENairaLKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
199-714 2.87e-19

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 91.82  E-value: 2.87e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILtcnA 278
Cdd:cd17642   46 SYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVF---C 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 279 VKRG-PKTINLkaivdaaldQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEwvdaEDPLFL 357
Cdd:cd17642  123 SKKGlQKVLNV---------QKKLKIIKTIIILDSKEDYKGYQCLYTFITQNLPPGFNEYDFKPPSFDRD----EQVALI 189
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHT--------------------TGGYMIYTATTFKYAFDYKSTDVYWCtadCGWitghsYVTYGPM 417
Cdd:cd17642  190 MNSSGSTGLPKGVQLThknivarfshardpifgnqiIPDTAILTVIPFHHGFGMFTTLGYLI---CGF-----RVVLMYK 261
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 418 LNGATVVvfegapnypdpgrcwDIVDKYKVSIFYTAPTLVRSLMRDddKFVTRHSRKSLRVLGSVGEPINPS---AWRWF 494
Cdd:cd17642  262 FEEELFL---------------RSLQDYKVQSALLVPTLFAFFAKS--TLVDKYDLSNLHEIASGGAPLSKEvgeAVAKR 324
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 495 FNVVGdsrcpISDTWWQTE-TGGFMITPlpgAWPQKPGSA--TFPFFGVQpVIVDEKGNEIEGECSGYLCVKGswPGAFR 571
Cdd:cd17642  325 FKLPG-----IRQGYGLTEtTSAILITP---EGDDKPGAVgkVVPFFYAK-VVDLDTGKTLGPNERGELCVKG--PMIMK 393
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 572 TLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVK 651
Cdd:cd17642  394 GYVNNPEATKALIDKD--GWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDA 471
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 652 GQGIYAFVTLLEGVPYSEelrKSLVLMVRNQIGAfAAPDR--IHWAPGLPKTRSGKIMRRILRKI 714
Cdd:cd17642  472 GELPAAVVVLEAGKTMTE---KEVMDYVASQVST-AKRLRggVKFVDEVPKGLTGKIDRRKIREI 532
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
183-711 5.78e-19

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 90.83  E-value: 5.78e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 183 TAIHWEGNELGVD--ASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:PRK13383  44 TAARWPGRTAIIDddGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDA 123
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILtCNavkrgpktinlkaivDAALDQSSKDGVSVgicLTYDNSLATTREntkwqngrdvwwqdvisqy 340
Cdd:PRK13383 124 LAAALRAHHISTVV-AD---------------NEFAERIAGADDAV---AVIDPATAGAEE------------------- 165
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptsCEVEWVDAEDPLFLLYTSGSTGKPKGVlhttggymiytattfKYAFDYKSTDVYWCT--ADCGWITGHSYVTYGPML 418
Cdd:PRK13383 166 ---SGGRPAVAAPGRIVLLTSGTTGKPKGV---------------PRAPQLRSAVGVWVTilDRTRLRTGSRISVAMPMF 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 NG-------------ATVVVFEgapnYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEP 485
Cdd:PRK13383 228 HGlglgmlmltialgGTVLTHR----HFDAEAALAQASLHRADAFTAVPVVLARILELPPRVRARNPLPQLRVVMSSGDR 303
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFFNVVGDSrcpISDTWWQTETG-GFMITPLP-GAWPQKPGSatfPFFGVQPVIVDEKGNEIEGECSGYLCVK 563
Cdd:PRK13383 304 LDPTLGQRFMDTYGDI---LYNGYGSTEVGiGALATPADlRDAPETVGK---PVAGCPVRILDRNNRPVGPRVTGRIFVG 377
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 564 GSWPGafrtlfgdhERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAA 642
Cdd:PRK13383 378 GELAG---------TRYTDGGGKAVVdGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNA 448
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 643 VVGIEHEVKGQGIYAFVTLLEGVPY-SEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK13383 449 VIGVPDERFGHRLAAFVVLHPGSGVdAAQLRDYL----KDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
195-711 8.93e-19

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 90.23  E-value: 8.93e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:cd17656   11 NQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVL 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 TCNAVKRGPKtinlkaivdaaldqsskdgvsvgicltyDNSLATTRENtkwqngrdvwwqDVISQYPTSCEVEWVDAEDP 354
Cdd:cd17656   91 TQRHLKSKLS----------------------------FNKSTILLED------------PSISQEDTSNIDYINNSDDL 130
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVL--HTTggymiyTATTFKYAFDYKSTDVYwctADCGWITGHSY-VTY----GPMLNGATVVVFE 427
Cdd:cd17656  131 LYIIYTSGTTGKPKGVQleHKN------MVNLLHFEREKTNINFS---DKVLQFATCSFdVCYqeifSTLLSGGTLYIIR 201
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 GAPNYpDPGRCWDIVDKYKVSIFYTaPTLVRSLMRDDDKFVTRHSrKSLRVLGSVGEPI---NPsawrwFFNVVGDSRCP 504
Cdd:cd17656  202 EETKR-DVEQLFDLVKRHNIEVVFL-PVAFLKFIFSEREFINRFP-TCVKHIITAGEQLvitNE-----FKEMLHEHNVH 273
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 505 ISDTWWQTET---GGFMITPLPgAWPQKPGSATfPFFGVQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRTLFGD----H 577
Cdd:cd17656  274 LHNHYGPSEThvvTTYTINPEA-EIPELPPIGK-PISNTWIYILDQEQQLQPQGIVGELYISGA--SVARGYLNRqeltA 349
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 578 ERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYA 657
Cdd:cd17656  350 EKFFPDPFDPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCA 429
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....
gi 378548265 658 FVTLLEGVPySEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17656  430 YFVMEQELN-ISQLREYL----AKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
180-713 1.82e-18

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 89.55  E-value: 1.82e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEgnelgvDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAD 259
Cdd:PRK08279  51 PDRPALLFE------DQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQRGA 124
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVIltcnavkrgpktinlkaIVDAALD---QSSKDGVSVGICLTYDNSLATTRENTkwqngrdvwWQDV 336
Cdd:PRK08279 125 VLAHSLNLVDAKHL-----------------IVGEELVeafEEARADLARPPRLWVAGGDTLDDPEG---------YEDL 178
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 337 ---ISQYPTSCEVEW--VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTAtTFKYAFDYKSTDVYWCT--------ADC 403
Cdd:PRK08279 179 aaaAAGAPTTNPASRsgVTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMG-GFGGLLRLTPDDVLYCClplyhntgGTV 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 404 GWitghsyvtyGPMLN-GATVVVFE--GAPNYpdpgrcWDIVDKYKVSIFYTAPTLVRSLM----RDDDKfvtRHsrkSL 476
Cdd:PRK08279 258 AW---------SSVLAaGATLALRRkfSASRF------WDDVRRYRATAFQYIGELCRYLLnqppKPTDR---DH---RL 316
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 477 RVLgsVGEPINPSAWRWFFNVVGDSRcpISDTWWQTEtGGFMITPLPGawpqKPGSATF-PFFGVQPV-IVD---EKGNE 551
Cdd:PRK08279 317 RLM--IGNGLRPDIWDEFQQRFGIPR--ILEFYAASE-GNVGFINVFN----FDGTVGRvPLWLAHPYaIVKydvDTGEP 387
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 552 IEGEcSGyLCVKGSwPGAFRTLFGdheryETTYFKPFAGY--------------------YF-SGDGCSRDKDGYYWLTG 610
Cdd:PRK08279 388 VRDA-DG-RCIKVK-PGEVGLLIG-----RITDRGPFDGYtdpeasekkilrdvfkkgdaWFnTGDLMRDDGFGHAQFVD 459
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 611 RVDDVI-----NVSghrigTAEVESALVLHPQCAEAAVVGIE-HEVKGQGIYAFVTLLEGVPYSeelRKSLVLMVRNQIG 684
Cdd:PRK08279 460 RLGDTFrwkgeNVA-----TTEVENALSGFPGVEEAVVYGVEvPGTDGRAGMAAIVLADGAEFD---LAALAAHLYERLP 531
                        570       580
                 ....*....|....*....|....*....
gi 378548265 685 AFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK08279 532 AYAVPLFVRLVPELETTGTFKYRKVDLRK 560
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
181-717 1.25e-17

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 86.44  E-value: 1.25e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIH-WEGnelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAvhsvVFAGFSAD 259
Cdd:cd05918   14 DAPAVCaWDG-------SLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGG----AFVPLDPS 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIV----DCKPNVILTCnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqd 335
Cdd:cd05918   83 HPLQRLQeilqDTGAKVVLTS----------------------------------------------------------- 103
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 336 visqyptscevewvDAEDPLFLLYTSGSTGKPKGVL--HTT--------GGYMIYTATT----F-KYAFDykstdvywct 400
Cdd:cd05918  104 --------------SPSDAAYVIFTSGSTGKPKGVVieHRAlstsalahGRALGLTSESrvlqFaSYTFD---------- 159
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 401 adcgwitGHSYVTYGPMLNGATVVVfegaPnyPDPGRCWDIVD---KYKVS-IFYTaPTLVRSLMRDDDkfvtrhsrKSL 476
Cdd:cd05918  160 -------VSILEIFTTLAAGGCLCI----P--SEEDRLNDLAGfinRLRVTwAFLT-PSVARLLDPEDV--------PSL 217
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 477 RVLGSVGEPINPSawrwffnVVgdsrcpisDTWWQ----------TETGGFMITPLPGAwPQKPGSATFPfFGVQPVIVD 546
Cdd:cd05918  218 RTLVLGGEALTQS-------DV--------DTWADrvrlinaygpAECTIAATVSPVVP-STDPRNIGRP-LGATCWVVD 280
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 547 EKGNE------------IEGE--CSGYL----CVKGSwpgafrtlFGDHERYETTYFKPFAGY-YFSGDGCSRDKDG--Y 605
Cdd:cd05918  281 PDNHDrlvpigavgellIEGPilARGYLndpeKTAAA--------FIEDPAWLKQEGSGRGRRlYRTGDLVRYNPDGslE 352
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 606 YwlTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGA 685
Cdd:cd05918  353 Y--VGRKDTQVKIRGQRVELGEIEHHLRQSLPGAKEVVVEVVKPKDGSSSPQLVAFVVLDGSSSGSGDGDSLFLEPSDEF 430
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|....*....
gi 378548265 686 FAAPDRI---------------HWAP--GLPKTRSGKIMRRILRKIASR 717
Cdd:cd05918  431 RALVAELrsklrqrlpsymvpsVFLPlsHLPLTASGKIDRRALRELAES 479
PRK07867 PRK07867
acyl-CoA synthetase; Validated
333-713 1.27e-17

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 86.66  E-value: 1.27e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 333 WQDVISQYP-TSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTG-----GYMIytATTFKYAFDykstDVYWCTAD---- 402
Cdd:PRK07867 132 WADELAAHRdAEPPFRVADPDDLFMLIFTSGTSGDPKAVRCTHRkvasaGVML--AQRFGLGPD----DVCYVSMPlfhs 205
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 403 ----CGWITGhsyvtygpMLNGATVVV---FEGAPNYPDpgrcwdiVDKYKVSIF-YTAPTLVRSLM---RDDDkfvtRH 471
Cdd:PRK07867 206 navmAGWAVA--------LAAGASIALrrkFSASGFLPD-------VRRYGATYAnYVGKPLSYVLAtpeRPDD----AD 266
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 472 SrkSLRVL-GSVGEPINPSAWRWFFNvvgdsrCPISDTWWQTEtGGFMITPLPGAwpqKPGSATFPFFGVQ--------- 541
Cdd:PRK07867 267 N--PLRIVyGNEGAPGDIARFARRFG------CVVVDGFGSTE-GGVAITRTPDT---PPGALGPLPPGVAivdpdtgte 334
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 542 --PVIVDEKGNEIEGECSGYLcVKGSWPGAFRTLFGDHEryeTTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVS 619
Cdd:PRK07867 335 cpPAEDADGRLLNADEAIGEL-VNTAGPGGFEGYYNDPE---ADAERMRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVD 410
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 620 GHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElRKSLVLMVRNQIGAFAAPDRIHWAPGLP 699
Cdd:PRK07867 411 GENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKFDPD-AFAEFLAAQPDLGPKQWPSYVRVCAELP 489
                        410
                 ....*....|....
gi 378548265 700 KTRSGKIMRRILRK 713
Cdd:PRK07867 490 RTATFKVLKRQLSA 503
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
197-713 1.33e-17

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 86.96  E-value: 1.33e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsvvFAGFSADSLAQRIvdckpnviltc 276
Cdd:PLN02330  55 AVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGV----FSGANPTALESEI----------- 119
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 277 navKRGPKTINLKAIVDAALDQSSKDGVSVGICLTYDNSLATTrentkwqngrdVWWQDVIS---QYPTSCEVEWVDAED 353
Cdd:PLN02330 120 ---KKQAEAAGAKLIVTNDTNYGKVKGLGLPVIVLGEEKIEGA-----------VNWKELLEaadRAGDTSDNEEILQTD 185
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLHT--------------TGGYMIYTATTFKYAFDYKstdVYWCTADCgwitghsyvtYGPMLN 419
Cdd:PLN02330 186 LCALPFSSGTTGISKGVMLThrnlvanlcsslfsVGPEMIGQVVTLGLIPFFH---IYGITGIC----------CATLRN 252
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATVVVfegapnypdpgrcwdiVDKYKVSIFYTA------------PTLVRSLMRDD--DKFVTrhSRKSLRVLGSVGEP 485
Cdd:PLN02330 253 KGKVVV----------------MSRFELRTFLNAlitqevsfapivPPIILNLVKNPivEEFDL--SKLKLQAIMTAAAP 314
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFfnvvgDSRCP---ISDTWWQTETGGFMIT---PLPGAWPQKPGSATFPFFGVQPVIVD-EKGNEIEGECSG 558
Cdd:PLN02330 315 LAPELLTAF-----EAKFPgvqVQEAYGLTEHSCITLThgdPEKGHGIAKKNSVGFILPNLEVKFIDpDTGRSLPKNTPG 389
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 559 YLCVKGSWpgAFRTLFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQC 638
Cdd:PLN02330 390 ELCVRSQC--VMQGYYNNKEETDRTIDE--DGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSV 465
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 639 AEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PLN02330 466 EDAAVVPLPDEEAGEIPAACVVINPKAKESEE---DILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKE 537
PRK06060 PRK06060
p-hydroxybenzoic acid--AMP ligase FadD22;
176-730 1.45e-17

p-hydroxybenzoic acid--AMP ligase FadD22;


Pssm-ID: 180374 [Multi-domain]  Cd Length: 705  Bit Score: 87.01  E-value: 1.45e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 176 EAGLGDKTAIHwegnelgVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAG 255
Cdd:PRK06060  16 EAGWYDRPAFY-------AADVVTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPE 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 256 FSADSLAQRIVDCKPNVILTCNAVKRGPKTINlkaIVDAA--LDQSSKDGVSvgicltydnslattrentkwqngrdvww 333
Cdd:PRK06060  89 LHRDDHALAARNTEPALVVTSDALRDRFQPSR---VAEAAelMSEAARVAPG---------------------------- 137
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 334 qdvisqyptscEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVT 413
Cdd:PRK06060 138 -----------GYEPMGGDALAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGNSV 206
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 414 YGPMLNGATVVVfEGAPNYPDPGRCwdIVDKYKVSIFYTAPTLVRSLMrdddKFVTRHSRKSLRVLGSVGEPINPS-AWR 492
Cdd:PRK06060 207 WFPLATGGSAVI-NSAPVTPEAAAI--LSARFGPSVLYGVPNFFARVI----DSCSPDSFRSLRCVVSAGEALELGlAER 279
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 493 W--FFNVVgdsrcPISDTWWQTETGGFMITPLPGAWpqKPGS--ATFPFFGVQPVIVD--EKGNEIEGEcsgyLCVKG-- 564
Cdd:PRK06060 280 LmeFFGGI-----PILDGIGSTEVGQTFVSNRVDEW--RLGTlgRVLPPYEIRVVAPDgtTAGPGVEGD----LWVRGpa 348
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 565 ------SWPgafRTLFGDheryettyfkpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQC 638
Cdd:PRK06060 349 iakgywNRP---DSPVAN------------EGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAV 413
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 639 AEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILR-KIASR 717
Cdd:PRK06060 414 AEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALRkQSPTK 493
                        570
                 ....*....|...
gi 378548265 718 QLEELGDTSTLAD 730
Cdd:PRK06060 494 PIWELSLTEPGSG 506
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
357-708 3.28e-17

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 83.32  E-value: 3.28e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 357 LLYTSGSTGKPKGVLhttggyMIYTATTFKYAfdykstdvYWCtaDCGWIT-GHSYVTYGP--------------MLNGA 421
Cdd:cd17638    5 IMFTSGTTGRSKGVM------CAHRQTLRAAA--------AWA--DCADLTeDDRYLIINPffhtfgykagivacLLTGA 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 422 TVV---VFegapnypDPGRCWDIVDKYKVSIFYTAPTLVRSLMR--DDDKFvtrhSRKSLRvlgsvgepinpsawrwfFN 496
Cdd:cd17638   69 TVVpvaVF-------DVDAILEAIERERITVLPGPPTLFQSLLDhpGRKKF----DLSSLR-----------------AA 120
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 VVGDSRCPISDTWWQTETGGFMITpLPGAWPQKPGSATFPFFGVQPVIVDEK------GNEIEGECSGYLCVKGswPGAF 570
Cdd:cd17638  121 VTGAATVPVELVRRMRSELGFETV-LTAYGLTEAGVATMCRPGDDAETVATTcgracpGFEVRIADDGEVLVRG--YNVM 197
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 RTLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:cd17638  198 QGYLDDPEATAEAIDAD--GWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDER 275
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 651 KGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17638  276 MGEVGKAFVVARPGVTLTEE---DVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
PRK12467 PRK12467
peptide synthase; Provisional
198-713 1.15e-16

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 85.21  E-value: 1.15e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCN 277
Cdd:PRK12467  538 LSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQS 617
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  278 AV-KRGPKTINLKAIVDAALDqsskdgvsvgicltydnslattrentkwqngrdvwwqDVISQYPTSCEVEWVDAEDPLF 356
Cdd:PRK12467  618 HLlAQLPVPAGLRSLCLDEPA-------------------------------------DLLCGYSGHNPEVALDPDNLAY 660
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  357 LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYafdykstdvYWCTADCGWITGHSY-------VTYGPMLNGATVVVFeGA 429
Cdd:PRK12467  661 VIYTSGSTGQPKGVAISHGALANYVCVIAER---------LQLAADDSMLMVSTFafdlgvtELFGALASGATLHLL-PP 730
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  430 PNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRhSRKSLRVLGSVGEPINPSAWRWF------FNVVGDSRC 503
Cdd:PRK12467  731 DCARDAEAFAALMADQGVTVLKIVPSHLQALLQASRVALPR-PQRALVCGGEALQVDLLARVRALgpgarlINHYGPTET 809
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  504 PISDTWWQTETGGFMITPLPGAWPQkPGSATFpffgvqpvIVDEKGNEIEGECSGYLCVKGSwpGAFRtlfGDHERYETT 583
Cdd:PRK12467  810 TVGVSTYELSDEERDFGNVPIGQPL-ANLGLY--------ILDHYLNPVPVGVVGELYIGGA--GLAR---GYHRRPALT 875
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  584 --YF--KPFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGI 655
Cdd:PRK12467  876 aeRFvpDPFGAdggrLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPGDAGLQLV 955
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265  656 YAFVTLL-----EGVPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK12467  956 AYLVPAAvadgaEHQATRDELKAQL----RQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPK 1014
PLN02479 PLN02479
acetate-CoA ligase
590-723 1.39e-16

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 83.74  E-value: 1.39e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 590 GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSE 669
Cdd:PLN02479 430 GWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSD 509
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 670 E--LRKSLVLMVRNQIGAFAAPDRIHWAPgLPKTRSGKIMRRILRKIAsrqlEELG 723
Cdd:PLN02479 510 EaaLAEDIMKFCRERLPAYWVPKSVVFGP-LPKTATGKIQKHVLRAKA----KEMG 560
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
198-709 2.58e-16

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 82.63  E-value: 2.58e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL--T 275
Cdd:PRK05852  44 ISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPALPIAEQRVRSQAAGARVVLidA 123
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 CNAVKRGPKTINLKAIvdaALDQSSKDGVSVGICL-TYDNSLATTRENTKWQNGRDvwwqdvisqyptscevewvdaeDP 354
Cdd:PRK05852 124 DGPHDRAEPTTRWWPL---TVNVGGDSGPSGGTLSvHLDAATEPTPATSTPEGLRP----------------------DD 178
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYmiytattfkyafdykSTDVYWCTADCGWITGHSYVTYGPMLNGATVV-------VFE 427
Cdd:PRK05852 179 AMIMFTGGTTGLPKMVPWTHANI---------------ASSVRAIITGYRLSPRDATVAVMPLYHGHGLIaallatlASG 243
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 GAPNYPDPGRC-----WDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGdsr 502
Cdd:PRK05852 244 GAVLLPARGRFsahtfWDDIKAVGATWYTAVPTIHQILLERAATEPSGRKPAALRFIRSCSAPLTAETAQALQTEFA--- 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 503 CPISDTWWQTET---------GGFMITPLPGAWPQKPGSATfpffGVQPVIVDEKGNEIEGECSGYLCVKGswPGAFRTL 573
Cdd:PRK05852 321 APVVCAFGMTEAthqvtttqiEGIGQTENPVVSTGLVGRST----GAQIRIVGSDGLPLPAGAVGEVWLRG--TTVVRGY 394
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 574 FGDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQ 653
Cdd:PRK05852 395 LGDPTITAANFTD---GWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGE 471
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 654 GIYAFVTLLEGVPYSeelRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRR 709
Cdd:PRK05852 472 AVAAVIVPRESAPPT---AEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
333-712 2.95e-16

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 82.38  E-value: 2.95e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 333 WQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGY-MIYTATTFKYafDYKSTDVYWCTADcgwiTGHS- 410
Cdd:PRK13388 131 YAELVAAAGALTPHREVDAMDPFMLIFTSGTTGAPKAVRCSHGRLaFAGRALTERF--GLTRDDVCYVSMP----LFHSn 204
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 --YVTYGPML-NGATVVV---FEGAPNYPDpgrcwdiVDKYKVSIF-YTAPTLVRSLM---RDDDkfvtrhSRKSLRV-L 479
Cdd:PRK13388 205 avMAGWAPAVaSGAAVALpakFSASGFLDD-------VRRYGATYFnYVGKPLAYILAtpeRPDD------ADNPLRVaF 271
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 GSVGEPINPSAWRWFFnvvgdsRCPISDTWWQTETGGfMITPLPGAwPqkPGSATFPFFGVqpVIVDEKGNEiegECSgy 559
Cdd:PRK13388 272 GNEASPRDIAEFSRRF------GCQVEDGYGSSEGAV-IVVREPGT-P--PGSIGRGAPGV--AIYNPETLT---ECA-- 334
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 560 lcvkgswpgafRTLFGDH-----------ERYETTYFKPFAGYY---------------FSGDGCSRDKDGYYWLTGRVD 613
Cdd:PRK13388 335 -----------VARFDAHgallnadeaigELVNTAGAGFFEGYYnnpeataermrhgmyWSGDLAYRDADGWIYFAGRTA 403
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 614 DVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQ--IGAFAAPDR 691
Cdd:PRK13388 404 DWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDPD---AFAAFLAAQpdLGTKAWPRY 480
                        410       420
                 ....*....|....*....|.
gi 378548265 692 IHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK13388 481 VRIAADLPSTATNKVLKRELI 501
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
193-645 3.55e-16

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 82.13  E-value: 3.55e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 193 GVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQrivdckpnv 272
Cdd:cd05932    2 GQVVEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRY--------- 72
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 ILTCNAVKrgpktinlkAIVDAALD--QSSKDGVSVGI--CLTYDNSLATTRENtkwqngrdvwWQDVISQYPTSCEVEW 348
Cdd:cd05932   73 VLEHSESK---------ALFVGKLDdwKAMAPGVPEGLisISLPPPSAANCQYQ----------WDDLIAQHPPLEERPT 133
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 349 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEG 428
Cdd:cd05932  134 RFPEQLATLIYTSGTTGQPKGVMLTFGSFA-WAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFAES 212
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 429 APNYPDPgrcwdiVDKYKVSIFYTAPTL-VRSLMRDDDK-----------------FVTRHSRKSL-----RVLGSVGEP 485
Cdd:cd05932  213 LDTFVED------VQRARPTLFFSVPRLwTKFQQGVQDKipqqklnlllkipvvnsLVKRKVLKGLgldqcRLAGCGSAP 286
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 486 INPSAWRWFFNVvgdsRCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEkgneiegecsGYLCVKGs 565
Cdd:cd05932  287 VPPALLEWYRSL----GLNILEAYGMTENFAYSHLNYPGR--DKIGTVGNAGPGVEVRISED----------GEILVRS- 349
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 566 wPGAFRTLFGDHERYETTYFKPfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVS-GHRIGTAEVESALVLHPQCAEAAVV 644
Cdd:cd05932  350 -PALMMGYYKDPEATAEAFTAD--GFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVI 426

                 .
gi 378548265 645 G 645
Cdd:cd05932  427 G 427
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
195-712 3.68e-16

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 82.38  E-value: 3.68e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDC--KPNV 272
Cdd:PRK07059  46 GKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSgaEAIV 125
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 273 IL-----TCNAVKrgPKTiNLKAIVDAAL-DQSSKDGVSVGICLtydnslattRENTK----WQNGRDVWWQDVISQ--- 339
Cdd:PRK07059 126 VLenfatTVQQVL--AKT-AVKHVVVASMgDLLGFKGHIVNFVV---------RRVKKmvpaWSLPGHVRFNDALAEgar 193
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 ---YPTScevewVDAEDPLFLLYTSGSTGKPKG--VLHTT-GGYMIYTATTFKYAFDYKSTD-------------VYWCT 400
Cdd:PRK07059 194 qtfKPVK-----LGPDDVAFLQYTGGTTGVSKGatLLHRNiVANVLQMEAWLQPAFEKKPRPdqlnfvcalplyhIFALT 268
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 401 AdCGWITghsyvtygpMLNGATVVVFegaPNYPD-PGRCWDIvDKYKVSIFYTAPTLVRSLMRDDDkFVTRHSRKSLRVL 479
Cdd:PRK07059 269 V-CGLLG---------MRTGGRNILI---PNPRDiPGFIKEL-KKYQVHIFPAVNTLYNALLNNPD-FDKLDFSKLIVAN 333
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 G---SVGEPInpsAWRWFfNVVGdsrCPISDTWWQTETGGfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GE 555
Cdd:PRK07059 334 GggmAVQRPV---AERWL-EMTG---CPITEGYGLSETSP-VATCNPVDATEFSGTIGLPLPSTEVSIRDDDGNDLPlGE 405
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 556 cSGYLCVKGSwpgafRTLFGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVL 634
Cdd:PRK07059 406 -PGEICIRGP-----QVMAGYWNRPDETAKVMTAdGFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVAS 479
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 635 HPQCAEAAVVGIEHEVKGQGIYAFVtllegvpyseeLRKSLVLmVRNQIGAFAA--------PDRIHWAPGLPKTRSGKI 706
Cdd:PRK07059 480 HPGVLEVAAVGVPDEHSGEAVKLFV-----------VKKDPAL-TEEDVKAFCKerltnykrPKFVEFRTELPKTNVGKI 547

                 ....*.
gi 378548265 707 MRRILR 712
Cdd:PRK07059 548 LRRELR 553
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
181-711 4.14e-16

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 81.71  E-value: 4.14e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNELgvdaslTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHsvvfagfsads 260
Cdd:cd17644   15 DAVAVVFEDQQL------TYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAY----------- 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 laqriVDCKPNViltcnavkrgPKTiNLKAIVDAAldqsskdGVSVgicltydnsLATTRENTkwqngrdvwwqdvisqy 340
Cdd:cd17644   78 -----VPLDPNY----------PQE-RLTYILEDA-------QISV---------LLTQPENL----------------- 108
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvdaedpLFLLYTSGSTGKPKGVL--------HTTGGYMIYTATT-------FKYAFDYKSTDVywctadcgw 405
Cdd:cd17644  109 --------------AYVIYTSGSTGKPKGVMiehqslvnLSHGLIKEYGITSsdrvlqfASIAFDVAAEEI--------- 165
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 406 itghsYVTygpMLNGATVVVfegAPN--YPDPGRCWDIVDKYKVSIFYTAPTLVRSLMrDDDKFVTRHSRKSLRVLGSVG 483
Cdd:cd17644  166 -----YVT---LLSGATLVL---RPEemRSSLEDFVQYIQQWQLTVLSLPPAYWHLLV-LELLLSTIDLPSSLRLVIVGG 233
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EPINPSAWR-W---------FFNVVGDSRCPISDTWwqtetggFMITPLPGAWPQKPGSATfPFFGVQPVIVDEKGNEIE 553
Cdd:cd17644  234 EAVQPELVRqWqknvgnfiqLINVYGPTEATIAATV-------CRLTQLTERNITSVPIGR-PIANTQVYILDENLQPVP 305
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 554 GECSG--YLCVKGSWPGAFRTLFGDHERYETTYF--KPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVE 629
Cdd:cd17644  306 VGVPGelHIGGVGLARGYLNRPELTAEKFISHPFnsSESERLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIE 385
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 630 SALVLHPQCAEAAVVGIEHEVKGQGIYAF-VTLLEGVPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd17644  386 AVLSQHNDVKTAVVIVREDQPGNKRLVAYiVPHYEESPSTVELRQFL----KAKLPDYMIPSAFVVLEELPLTPNGKIDR 461

                 ...
gi 378548265 709 RIL 711
Cdd:cd17644  462 RAL 464
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
192-715 6.26e-16

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 81.41  E-value: 6.26e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 192 LGVdaSLTYSELLQRVCQLANYL-KDNGVKKGDAVVIYLPMLMELPIAMLACARIGAV--------------HSVVFAGF 256
Cdd:PRK12492  46 LGV--TLSYAELERHSAAFAAYLqQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIvvntnplytaremrHQFKDSGA 123
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SA----DSLAQRIVDCKPN-VILTCNAVKRGPKTINLKA-IVDAALDQSSK--DGVSVGICLTYDNSLattrentkwQNG 328
Cdd:PRK12492 124 RAlvylNMFGKLVQEVLPDtGIEYLIEAKMGDLLPAAKGwLVNTVVDKVKKmvPAYHLPQAVPFKQAL---------RQG 194
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 329 RDvwwqdvISQYPTScevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTD------------- 395
Cdd:PRK12492 195 RG------LSLKPVP-----VGLDDIAVLQYTGGTTGLAKGAMLTHGN-LVANMLQVRACLSQLGPDgqplmkegqevmi 262
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 396 -------VYWCTADCGWItghsyvtygpMLNGATVVVFEgapNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFV 468
Cdd:PRK12492 263 aplplyhIYAFTANCMCM----------MVSGNHNVLIT---NPRDIPGFIKELGKWRFSALLGLNTLFVALMDHPGFKD 329
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 469 TRHSRksLRVLGSVGEP-INPSAWRWFfNVVGdsrCPISDTWWQTETggfmiTPLPGAWP----QKPGSATFPFFGVQPV 543
Cdd:PRK12492 330 LDFSA--LKLTNSGGTAlVKATAERWE-QLTG---CTIVEGYGLTET-----SPVASTNPygelARLGTVGIPVPGTALK 398
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 IVDEKGNEIE-GEcSGYLCVKGSwpgafRTLFGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGH 621
Cdd:PRK12492 399 VIDDDGNELPlGE-RGELCIKGP-----QVMKGYWQQPEATAEALDAeGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGF 472
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 622 RIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKslvlMVRNQIGAFAAPDRIHWAPGLPKT 701
Cdd:PRK12492 473 NVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDPGLSVEELKA----YCKENFTGYKVPKHIVLRDSLPMT 548
                        570
                 ....*....|....
gi 378548265 702 RSGKIMRRILRKIA 715
Cdd:PRK12492 549 PVGKILRRELRDIA 562
PLN03102 PLN03102
acyl-activating enzyme; Provisional
198-715 8.36e-16

acyl-activating enzyme; Provisional


Pssm-ID: 215576 [Multi-domain]  Cd Length: 579  Bit Score: 81.22  E-value: 8.36e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILtcn 277
Cdd:PLN03102  40 FTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILF--- 116
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 aVKRgpktiNLKAIVDAALDQSSKDGVSVGICLTYDNSLattrENTKWQNGRDVWWQDVISQ-YPTSCEVEWV----DAE 352
Cdd:PLN03102 117 -VDR-----SFEPLAREVLHLLSSEDSNLNLPVIFIHEI----DFPKRPSSEELDYECLIQRgEPTPSLVARMfriqDEH 186
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHT-TGGYMiyTATTFKYAFDYKSTDVYWCTA---DC-GWItghsyVTYGPMLNGATVVVFE 427
Cdd:PLN03102 187 DPISLNYTSGTTADPKGVVIShRGAYL--STLSAIIGWEMGTCPVYLWTLpmfHCnGWT-----FTWGTAARGGTSVCMR 259
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 --GAPnypdpgRCWDIVDKYKVSIFYTAPTLVRSLMRDDDkfvTRHSRKSLRVLGSVGEPINPSAW-----RWFFNVVgd 500
Cdd:PLN03102 260 hvTAP------EIYKNIEMHNVTHMCCVPTVFNILLKGNS---LDLSPRSGPVHVLTGGSPPPAALvkkvqRLGFQVM-- 328
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 501 srcpisDTWWQTE-TGGFMITPLPGAWPQKPGSATFPFFGVQPVI------VDEKGNEI------EGECSGYLCVKGSwp 567
Cdd:PLN03102 329 ------HAYGLTEaTGPVLFCEWQDEWNRLPENQQMELKARQGVSilgladVDVKNKETqesvprDGKTMGEIVIKGS-- 400
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 gafrTLFGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGI 646
Cdd:PLN03102 401 ----SIMKGYLKNPKATSEAFKhGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAM 476
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 647 EHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIG-------AFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:PLN03102 477 PHPTWGETPCAFVVLEKGETTKEDRVDKLVTRERDLIEycrenlpHFMCPRKVVFLQELPKNGNGKILKPKLRDIA 552
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
195-720 1.98e-15

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 80.86  E-value: 1.98e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIL 274
Cdd:PRK10252  481 RYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLI 560
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  275 TcnavkrgpktinlkaivdAALDQSSKDGVSVGICLTYDNSLATtrentkwqngrdvwwQDVISQYPTSCevewvdaEDP 354
Cdd:PRK10252  561 T------------------TADQLPRFADVPDLTSLCYNAPLAP---------------QGAAPLQLSQP-------HHT 600
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  355 LFLLYTSGSTGKPKGVL--HT---------------TGGYMIYTATtfKYAFDYKSTDVYWctadcgwitghsyvtygPM 417
Cdd:PRK10252  601 AYIIFTSGSTGRPKGVMvgQTaivnrllwmqnhyplTADDVVLQKT--PCSFDVSVWEFFW-----------------PF 661
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  418 LNGATVVVfegAP--NYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWF- 494
Cdd:PRK10252  662 IAGAKLVM---AEpeAHRDPLAMQQFFAEYGVTTTHFVPSMLAAFVASLTPEGARQSCASLRQVFCSGEALPADLCREWq 738
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  495 -------FNVVGDSRCPISDTWWqtetggfmitPLPGAWPQKPGSAT----FPFFGVQPVIVDEKGNEIEGECSGYLCVK 563
Cdd:PRK10252  739 qltgaplHNLYGPTEAAVDVSWY----------PAFGEELAAVRGSSvpigYPVWNTGLRILDARMRPVPPGVAGDLYLT 808
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  564 GswpgafRTLF-GDHERYETTY-------FKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLH 635
Cdd:PRK10252  809 G------IQLAqGYLGRPDLTAsrfiadpFAPGERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQAL 882
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  636 PQCAEA---AVVGIEHEVKGQG---IYAFVTLLEGVPY-SEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:PRK10252  883 PDVEQAvthACVINQAAATGGDarqLVGYLVSQSGLPLdTSALQAQL----RERLPPHMVPVVLLQLDQLPLSANGKLDR 958
                         570
                  ....*....|....*...
gi 378548265  709 RIL------RKIASRQLE 720
Cdd:PRK10252  959 KALplpelkAQVPGRAPK 976
A_NRPS_acs4 cd17654
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ...
191-706 2.16e-15

acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341309 [Multi-domain]  Cd Length: 449  Bit Score: 79.44  E-value: 2.16e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 191 ELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsvvFAGFSADSLAQRIVDCKP 270
Cdd:cd17654   10 QTTSDTTVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAA----YAPIDPASPEQRSLTVMK 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 271 NVILTCNAVKRgpKTINLKAIVDAALDQSSKdgvSVGICLTYdnslattrentkwqngrdvwwqdvisqyptscevewvd 350
Cdd:cd17654   86 KCHVSYLLQNK--ELDNAPLSFTPEHRHFNI---RTDECLAY-------------------------------------- 122
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 aedplfLLYTSGSTGKPKGVlHTTGGYMIYTATTFKYAFDYKSTDVYWCTadcgwitghSYVTYGP--------MLNGAT 422
Cdd:cd17654  123 ------VIHTSGTTGTPKIV-AVPHKCILPNIQHFRSLFNITSEDILFLT---------SPLTFDPsvveiflsLSSGAT 186
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 423 VVVFEGAPNyPDPGRCWDIVDK-YKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPInPS-----AWRWFFN 496
Cdd:cd17654  187 LLIVPTSVK-VLPSKLADILFKrHRITVLQATPTLFRRFGSQSIKSTVLSATSSLRVLALGGEPF-PSlvilsSWRGKGN 264
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 vvgdsRCPISDTWWQTETGGFMIT--------PLPGAwpqkpgsatFPFFGVQPVIVDEKGNEIEGEcsgyLCVKGSWPG 568
Cdd:cd17654  265 -----RTRIFNIYGITEVSCWALAykvpeedsPVQLG---------SPLLGTVIEVRDQNGSEGTGQ----VFLGGLNRV 326
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 AFRtlfgdhERYETTyfkPFAGYYFSGDGCSRdKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQcAEAAVVGIEh 648
Cdd:cd17654  327 CIL------DDEVTV---PKGTMRATGDFVTV-KDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLG-VESCAVTLS- 394
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 649 evKGQGIYAFVTlleGVPYSEELRKSLVLmvrNQIGAFAAPDRIHWAPGLPKTRSGKI 706
Cdd:cd17654  395 --DQQRLIAFIV---GESSSSRIHKELQL---TLLSSHAIPDTFVQIDKLPLTSHGKV 444
PRK07008 PRK07008
long-chain-fatty-acid--CoA ligase; Validated
338-712 2.28e-15

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235908 [Multi-domain]  Cd Length: 539  Bit Score: 79.75  E-value: 2.28e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 338 SQYPTSCEVEWVDAED-----PLF-------LLYTSGSTGKPKGVLHTTGGYMIYT-ATTFKYAFDYKSTDVY------- 397
Cdd:PRK07008 150 GSTPLLCYETLVGAQDgdydwPRFdenqassLCYTSGTTGNPKGALYSHRSTVLHAyGAALPDAMGLSARDAVlpvvpmf 229
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 398 ----WCTADCGWITGHSYVTYGPMLNGATVvvfegapnypdpgrcWDIVDKYKVSIFYTAPT----LVRSLMRDDDKFVT 469
Cdd:PRK07008 230 hvnaWGLPYSAPLTGAKLVLPGPDLDGKSL---------------YELIEAERVTFSAGVPTvwlgLLNHMREAGLRFST 294
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 470 rhsrksLR--VLGSVGEPinPSAWRWFFNVVGdsrCPISDTWWQTETGGF-MITPLPGAWPQKPGSATF--------PFF 538
Cdd:PRK07008 295 ------LRrtVIGGSACP--PAMIRTFEDEYG---VEVIHAWGMTEMSPLgTLCKLKWKHSQLPLDEQRkllekqgrVIY 363
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 539 GVQPVIVDEKGNEI--EGECSGYLCVKGSWpgafrtlfgdherYETTYFK----PF-AGYYFSGDGCSRDKDGYYWLTGR 611
Cdd:PRK07008 364 GVDMKIVGDDGRELpwDGKAFGDLQVRGPW-------------VIDRYFRgdasPLvDGWFPTGDVATIDADGFMQITDR 430
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 612 VDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVpysEELRKSLVLMVRNQIGAFAAPDR 691
Cdd:PRK07008 431 SKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGA---EVTREELLAFYEGKVAKWWIPDD 507
                        410       420
                 ....*....|....*....|.
gi 378548265 692 IHWAPGLPKTRSGKIMRRILR 712
Cdd:PRK07008 508 VVFVDAIPHTATGKLQKLKLR 528
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
181-711 2.30e-15

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 79.14  E-value: 2.30e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGNelgvdaSLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADS 260
Cdd:cd17645   13 DHVAVVDRGQ------SLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGER 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVDCKPNVILTcnavkrgpktinlkaivdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqy 340
Cdd:cd17645   87 IAYMLADSSAKILLT----------------------------------------------------------------- 101
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 341 ptscevewvDAEDPLFLLYTSGSTGKPKGVL--HTTggyMIYTATTFKYAFDYKSTDVYWCTADCGWiTGHSYVTYGPML 418
Cdd:cd17645  102 ---------NPDDLAYVIYTSGSTGLPKGVMieHHN---LVNLCEWHRPYFGVTPADKSLVYASFSF-DASAWEIFPHLT 168
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 NGATVVVFEGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKfvtrhsrkSLRVLGSVGEPINPSAWRWF--FN 496
Cdd:cd17645  169 AGAALHVVPSERRL-DLDALNDYFNQEGITISFLPTGAAEQFMQLDNQ--------SLRVLLTGGDKLKKIERKGYklVN 239
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 497 VVGDSRCPIsdtwwqtetggfMITPLPGAWPQKPGSATFPFFGVQPVIVDEkGNEIEGE-CSGYLCVKGSwpGAFRTLFG 575
Cdd:cd17645  240 NYGPTENTV------------VATSFEIDKPYANIPIGKPIDNTRVYILDE-ALQLQPIgVAGELCIAGE--GLARGYLN 304
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 576 ----DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVK 651
Cdd:cd17645  305 rpelTAEKFIVHPFVPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADG 384
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 652 GQGIYAFVTLLEGVPYsEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17645  385 RKYLVAYVTAPEEIPH-EELREWL----KNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
hsFATP4_like cd05939
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ...
195-713 3.70e-15

Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341262 [Multi-domain]  Cd Length: 474  Bit Score: 78.62  E-value: 3.70e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIvdckpnvil 274
Cdd:cd05939    1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCI--------- 71
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 tcnavkrgpKTINLKAIVDAALDqsskdgvsvgicltydnSLATTrentkwqngrdvwwqdvISQYPTSCEVewVDAEDP 354
Cdd:cd05939   72 ---------TVSKAKALIFNLLD-----------------PLLTQ-----------------SSTEPPSQDD--VNFRDK 106
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 LFLLYTSGSTGKPKGVLHTTGGYMIYTATTFkYAFDYKSTDV-YWC-----TAdcGWITGHSYVtygpMLNGATVVVFE- 427
Cdd:cd05939  107 LFYIYTSGTTGLPKAAVIVHSRYYRIAAGAY-YAFGMRPEDVvYDClplyhSA--GGIMGVGQA----LLHGSTVVIRKk 179
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 428 -GAPNYpdpgrcWDIVDKYKVSIFYTAPTLVRSLM----RDDDKfvtRHsrkslRVLGSVGEPINPSAWRWF---FNV-- 497
Cdd:cd05939  180 fSASNF------WDDCVKYNCTIVQYIGEICRYLLaqppSEEEQ---KH-----NVRLAVGNGLRPQIWEQFvrrFGIpq 245
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 498 ----VGDSRCPISDTWWQTETG--GFMITPLPGAWPqkpgsatfpffgVQPVIVDEKGNEIEGECSGY--LCVKGSwPGA 569
Cdd:cd05939  246 igefYGATEGNSSLVNIDNHVGacGFNSRILPSVYP------------IRLIKVDEDTGELIRDSDGLciPCQPGE-PGL 312
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 570 F----------RTLFG------DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV 633
Cdd:cd05939  313 LvgkiiqndplRRFDGyvnegaTNKKIARDVFKKGDSAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILS 392
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 634 LHPQCAEAAVVGIE-HEVKGQ-GIYAFVTLLEGVP---YSEELRKSLVlmvrnqigAFAAPDRIHWAPGLPKTRSGKIMR 708
Cdd:cd05939  393 NVLGLEDVVVYGVEvPGVEGRaGMAAIVDPERKVDldrFSAVLAKSLP--------PYARPQFIRLLPEVDKTGTFKLQK 464

                 ....*
gi 378548265 709 RILRK 713
Cdd:cd05939  465 TDLQK 469
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
197-715 4.46e-15

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 78.77  E-value: 4.46e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 197 SLTYSELLQRVCQLANYL-KDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILT 275
Cdd:PRK08751  50 TITYREADQLVEQFAAYLlGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVV 129
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 ----CNAVKRGPKTINLKAIVDAALDQ--SSKDGVSVGICLTYDNSLATtrentKWQNGRDVWWQDVISQ-YPTSCEVEW 348
Cdd:PRK08751 130 idnfGTTVQQVIADTPVKQVITTGLGDmlGFPKAALVNFVVKYVKKLVP-----EYRINGAIRFREALALgRKHSMPTLQ 204
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 349 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKyafdykstdvyWCTADCGWITGHSYV-TYGPML-------NG 420
Cdd:PRK08751 205 IEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQ-----------WLAGTGKLEEGCEVViTALPLYhifaltaNG 273
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 421 ATVVVFEGA----PNYPD-PGRCWDIvDKYKVSIFYTAPTLVRSLMR----DDDKFvtrhsrKSLRVLGSVGEPINPS-A 490
Cdd:PRK08751 274 LVFMKIGGCnhliSNPRDmPGFVKEL-KKTRFTAFTGVNTLFNGLLNtpgfDQIDF------SSLKMTLGGGMAVQRSvA 346
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 491 WRWFfNVVGdsrCPISDTWWQTETG-GFMITPLpgAWPQKPGSATFPFFGVQPVIVDEKGNEIE-GECsGYLCVKGSwpg 568
Cdd:PRK08751 347 ERWK-QVTG---LTLVEAYGLTETSpAACINPL--TLKEYNGSIGLPIPSTDACIKDDAGTVLAiGEI-GELCIKGP--- 416
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 afRTLFGDHER-YETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:PRK08751 417 --QVMKGYWKRpEETAKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVP 494
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEELRKSlvlmVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:PRK08751 495 DEKSGEIVKVVIVKKDPALTAEDVKAH----ARANLTGYKQPRIIEFRKELPKTNVGKILRRELRDAA 558
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
194-719 5.87e-15

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 78.27  E-value: 5.87e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 194 VDASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVhsVVFAG--FSA--------DSLA 262
Cdd:PRK05677  46 LGKTLTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLI--VVNTNplYTAremehqfnDSGA 123
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 263 QRIVdCKPNviLTCNAVKRGPKTINLKAIVDAALDQSSK-DGVSVGICLTYDNSLATTrentkWQNGRDVWWQDVISQ-- 339
Cdd:PRK05677 124 KALV-CLAN--MAHLAEKVLPKTGVKHVIVTEVADMLPPlKRLLINAVVKHVKKMVPA-----YHLPQAVKFNDALAKga 195
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 340 YPTSCEVEwVDAEDPLFLLYTSGSTGKPKGVLHT-------------------TGGYMIYTATTFKYafdykstDVYWCT 400
Cdd:PRK05677 196 GQPVTEAN-PQADDVAVLQYTGGTTGVAKGAMLThrnlvanmlqcralmgsnlNEGCEILIAPLPLY-------HIYAFT 267
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 401 ADCGWItghsyvtygpMLNGATVVVFegaPNYPD-PGRCWDIvDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRksLRVL 479
Cdd:PRK05677 268 FHCMAM----------MLIGNHNILI---SNPRDlPAMVKEL-GKWKFSGFVGLNTLFVALCNNEAFRKLDFSA--LKLT 331
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 480 GSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTETggfmiTPLPGAWPQK---PGSATFPFFGVQPVIVDEKGNEIEGEC 556
Cdd:PRK05677 332 LSGGMALQLATAERWKEVTG---CAICEGYGMTET-----SPVVSVNPSQaiqVGTIGIPVPSTLCKVIDDDGNELPLGE 403
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 557 SGYLCVKGswPGAFRtlfGDHERYETTYFKPFA-GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLH 635
Cdd:PRK05677 404 VGELCVKG--PQVMK---GYWQRPEATDEILDSdGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAAL 478
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 636 PQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIA 715
Cdd:PRK05677 479 PGVLQCAAIGVPDEKSGEAIKVFVVVKPGETLTKE---QVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELRDEE 555

                 ....
gi 378548265 716 SRQL 719
Cdd:PRK05677 556 LKKA 559
PRK05620 PRK05620
long-chain fatty-acid--CoA ligase;
180-713 6.25e-15

long-chain fatty-acid--CoA ligase;


Pssm-ID: 180167 [Multi-domain]  Cd Length: 576  Bit Score: 78.29  E-value: 6.25e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 180 GDKTAIHWEGNELgvdASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSA 258
Cdd:PRK05620  24 GDTTVTTWGGAEQ---EQTTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMN 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 259 DSL-------AQRIVDCKP-------NVILTCNAVKR----GPKTINLKAIVDAaldqsskDGVSvgiCLTYDNSLattr 320
Cdd:PRK05620 101 DQIvhiinhaEDEVIVADPrlaeqlgEILKECPCVRAvvfiGPSDADSAAAHMP-------EGIK---VYSYEALL---- 166
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 321 entkwqNGRdvwwqdvisqyptSCEVEW--VDAEDPLFLLYTSGSTGKPKGV-------------LHTTGGYMIYTATTF 385
Cdd:PRK05620 167 ------DGR-------------STVYDWpeLDETTAAAICYSTGTTGAPKGVvyshrslylqslsLRTTDSLAVTHGESF 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 386 kyafdYKSTDVY----WCTADCGWITGHSYVTYGPMLNGATVVvfegapnypdpgrcwDIVDKYKVSIFYTAPTLVRSLM 461
Cdd:PRK05620 228 -----LCCVPIYhvlsWGVPLAAFMSGTPLVFPGPDLSAPTLA---------------KIIATAMPRVAHGVPTLWIQLM 287
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 462 RDDDKfvTRHSRKSLRVLGSVGEPINP---SAWRWFFNVvgdsrcPISDTWWQTETG--GFMITPLPGA-----WPQKPG 531
Cdd:PRK05620 288 VHYLK--NPPERMSLQEIYVGGSAVPPiliKAWEERYGV------DVVHVWGMTETSpvGTVARPPSGVsgearWAYRVS 359
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 532 SATFPFfGVQPVIVDEkGNEIEG--ECSGYLCVKGSW-------------PGAFRTLFGDHERYETTYFKPfAGYYFSGD 596
Cdd:PRK05620 360 QGRFPA-SLEYRIVND-GQVMEStdRNEGEIQVRGNWvtasyyhspteegGGAASTFRGEDVEDANDRFTA-DGWLRTGD 436
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 597 GCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLV 676
Cdd:PRK05620 437 VGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETAERLR 516
                        570       580       590
                 ....*....|....*....|....*....|....*..
gi 378548265 677 LMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK05620 517 DQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLRQ 553
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
353-704 3.41e-14

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 74.26  E-value: 3.41e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 353 DPLFLLYTSGSTGKPKGVLHTTGGYMIYTattfkyafdykstdvyWCTADCGWITGHS-YVTYGPM-----LNGATVVVF 426
Cdd:cd17636    1 DPVLAIYTAAFSGRPNGALLSHQALLAQA----------------LVLAVLQAIDEGTvFLNSGPLfhigtLMFTLATFH 64
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 427 EGAPNY----PDPGRCWDIVDKYKVS-IFYTAPTL--VRSLMRDDdkfvtRHSRKSLRVLgsvgepinPSAWRWFFNVvg 499
Cdd:cd17636   65 AGGTNVfvrrVDAEEVLELIEAERCThAFLLPPTIdqIVELNADG-----LYDLSSLRSS--------PAAPEWNDMA-- 129
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 500 dsrcPISDTWW--------QTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEI-EGEcSGYLCVKGswPGAF 570
Cdd:cd17636  130 ----TVDTSPWgrkpggygQTEVMGLATFAALGG--GAIGGAGRPSPLVQVRILDEDGREVpDGE-VGEIVARG--PTVM 200
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 571 RTLFGdheRYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 650
Cdd:cd17636  201 AGYWN---RPEVNARRTRGGWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPR 277
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....
gi 378548265 651 KGQGIYAFVTLLEGVPYSEElrkSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSG 704
Cdd:cd17636  278 WAQSVKAIVVLKPGASVTEA---ELIEHCRARIASYKKPKSVEFADALPRTAGG 328
FATP_chFAT1_like cd05937
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ...
199-713 6.89e-14

Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.


Pssm-ID: 341260 [Multi-domain]  Cd Length: 468  Bit Score: 74.78  E-value: 6.89e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLaqriVDCkpnvILTCN 277
Cdd:cd05937    7 TYSETYDLVLRYAHWLHDDlGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPL----IHC----LKLSG 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 AvkrgpktinlKAIVdaaldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewVDAEDPLFL 357
Cdd:cd05937   79 S----------RFVI--------------------------------------------------------VDPDDPAIL 92
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGgyMIY-TATTFKYAFDYKSTD-VYWC----------TADCGWITGHSYVTYGP--------- 416
Cdd:cd05937   93 IYTSGTTGLPKAAAISWR--RTLvTSNLLSHDLNLKNGDrTYTCmplyhgtaafLGACNCLMSGGTLALSRkfsasqfwk 170
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 417 --MLNGATVVVFEG--------APNYPDP-----------GRCWDIVDKYK----VSI---FYTAPTLVRSLmrdddkfv 468
Cdd:cd05937  171 dvRDSGATIIQYVGelcryllsTPPSPYDrdhkvrvawgnGLRPDIWERFRerfnVPEigeFYAATEGVFAL-------- 242
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 469 TRHSRKSLrVLGSVGEpiNPSAWRWFFN-----VVGDsrcPISDTWWQTETGGFMI-TPLpgawpQKPGSATFPFFGVQp 542
Cdd:cd05937  243 TNHNVGDF-GAGAIGH--HGLIRRWKFEnqvvlVKMD---PETDDPIRDPKTGFCVrAPV-----GEPGEMLGRVPFKN- 310
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 543 vivdekgneiegecsgylcvKGSWPGAFRTLFGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHR 622
Cdd:cd05937  311 --------------------REAFQGYLHNEDATESKLVRDVFRKGDIYFRTGDLLRQDADGRWYFLDRLGDTFRWKSEN 370
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 623 IGTAEVESALVLHPQCAEAAVVGIE---HEvkGQGIYAFVTLLEGVPYSEELRKS-LVLMVRNQIGAFAAPDRIHWAPGL 698
Cdd:cd05937  371 VSTTEVADVLGAHPDIAEANVYGVKvpgHD--GRAGCAAITLEESSAVPTEFTKSlLASLARKNLPSYAVPLFLRLTEEV 448
                        570
                 ....*....|....*
gi 378548265 699 PKTRSGKIMRRILRK 713
Cdd:cd05937  449 ATTDNHKQQKGVLRD 463
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
177-714 7.39e-14

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 75.01  E-value: 7.39e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 177 AGLGDKTAIHWEGNELGVDAsLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACarigavhsvVFAGF 256
Cdd:cd05906   20 AERGPTKGITYIDADGSEEF-QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWAC---------VLAGF 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 257 SAdslaqrivdckpnVILTCNAVKRGPKTINLKAI-VDAALDQsskdgvsvGICLTYDNSLATTRENTKWQnGRDVWWQD 335
Cdd:cd05906   90 VP-------------APLTVPPTYDEPNARLRKLRhIWQLLGS--------PVVLTDAELVAEFAGLETLS-GLPGIRVL 147
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 336 VISQYP-TSCEVEWV--DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKyAFDYKSTDVY--WCTADcgWITGHS 410
Cdd:cd05906  148 SIEELLdTAADHDLPqsRPDDLALLMLTSGSTGFPKAVPLTHRNILARSAGKIQ-HNGLTPQDVFlnWVPLD--HVGGLV 224
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 411 YVTYGPMLNG------ATVVVFEgapnypDPGRCWDIVDKYKVSIFYtAPTLVRSLMRDD-DKFVTRH-SRKSLRVLGSV 482
Cdd:cd05906  225 ELHLRAVYLGcqqvhvPTEEILA------DPLRWLDLIDRYRVTITW-APNFAFALLNDLlEEIEDGTwDLSSLRYLVNA 297
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 483 GEPINPSAWRWFFNVVGDSRCP---ISDTWWQTETGGFMITPLPGAWPQKPGSATF-----PFFGVQPVIVDEKGNEIEG 554
Cdd:cd05906  298 GEAVVAKTIRRLLRLLEPYGLPpdaIRPAFGMTETCSGVIYSRSFPTYDHSQALEFvslgrPIPGVSMRIVDDEGQLLPE 377
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 555 ECSGYLCVKGswPGAFRTLFGDHERYETTYFKpfAGYYFSGD-GCSRDkdGYYWLTGRVDDVINVSGHRIGTAEVESAL- 632
Cdd:cd05906  378 GEVGRLQVRG--PVVTKGYYNNPEANAEAFTE--DGWFRTGDlGFLDN--GNLTITGRTKDTIIVNGVNYYSHEIEAAVe 451
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 633 ---VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGafAAPDR-IHWAPG-LPKTRSGKIM 707
Cdd:cd05906  452 evpGVEPSFTAAFAVRDPGAETEELAIFFVPEYDLQDALSETLRAIRSVVSREVG--VSPAYlIPLPKEeIPKTSLGKIQ 529

                 ....*..
gi 378548265 708 RRILRKI 714
Cdd:cd05906  530 RSKLKAA 536
PRK05857 PRK05857
fatty acid--CoA ligase;
196-711 1.47e-13

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 73.89  E-value: 1.47e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 196 ASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILT 275
Cdd:PRK05857  40 SALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPIAAIERFCQITDPAAALV 119
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 CNAVKRGPKTI--NLKAIVDAALDQSSKDGVSVgicltydNSLATTRENTKWQNGrdvwwqdvisqyptscevewvdAED 353
Cdd:PRK05857 120 APGSKMASSAVpeALHSIPVIAVDIAAVTRESE-------HSLDAASLAGNADQG----------------------SED 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 354 PLFLLYTSGSTGKPKGVLhttggymIYTATTFKYAFDYKSTDVYWCTadcgWITGHSyvTYGPM---------------L 418
Cdd:PRK05857 171 PLAMIFTSGTTGEPKAVL-------LANRTFFAVPDILQKEGLNWVT----WVVGET--TYSPLpathigglwwiltclM 237
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 419 NGATVVVfeGAPNypdPGRCWDIVDKYKVSIFYTAPTLVRSLMrDDDKFvTRHSRKSLRVLGSVGEPINPSAWRwFFNVV 498
Cdd:PRK05857 238 HGGLCVT--GGEN---TTSLLEILTTNAVATTCLVPTLLSKLV-SELKS-ANATVPSLRLVGYGGSRAIAADVR-FIEAT 309
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 GdsrCPISDTWWQTETGGFMITpLP---GAWPQ-KPGSATFPFFGVQPVIVDEKG---NEIEGECS---GYLCVKGswPG 568
Cdd:PRK05857 310 G---VRTAQVYGLSETGCTALC-LPtddGSIVKiEAGAVGRPYPGVDVYLAATDGigpTAPGAGPSasfGTLWIKS--PA 383
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 569 AFRTLFGDHERYETTYFKpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEH 648
Cdd:PRK05857 384 NMLGYWNNPERTAEVLID---GWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPD 460
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 649 EVKGqgiyAFVTLLEgVPYSE-------ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK05857 461 EEFG----ALVGLAV-VASAEldesaarALKHTIAARFRRESEPMARPSTIVIVTDIPRTQSGKVMRASL 525
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
585-713 1.11e-12

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 70.07  E-value: 1.11e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 585 FKPFA--GYYFSGDGCSRDkDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVtll 662
Cdd:PRK07824 227 PDPFAepGWFRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAV--- 302
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 378548265 663 EGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713
Cdd:PRK07824 303 VGDGGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
FATP_FACS cd05940
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ...
195-647 1.26e-12

Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341263 [Multi-domain]  Cd Length: 449  Bit Score: 70.46  E-value: 1.26e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 195 DASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVIl 274
Cdd:cd05940    1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHL- 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 275 tcnavkrgpktinlkaIVDAAldqsskdgvsvgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaedp 354
Cdd:cd05940   80 ----------------VVDAA----------------------------------------------------------- 84
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 355 lFLLYTSGSTGKPKGVLHTTGGYmIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFE--GAPNY 432
Cdd:cd05940   85 -LYIYTSGTTGLPKAAIISHRRA-WRGGAFFAGSGGALPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRKkfSASNF 162
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 433 pdpgrcWDIVDKYKVSIFYTAPTLVRSLM----RDDDKfvtRHsrkSLRVLgsVGEPINPSAWRWFFNVVGDSRcpISDT 508
Cdd:cd05940  163 ------WDDIRKYQATIFQYIGELCRYLLnqppKPTER---KH---KVRMI--FGNGLRPDIWEEFKERFGVPR--IAEF 226
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 509 WWQTE--TGGFMITPLPGAWPQKPgSATFPFFGVQPVIVD-EKGNEIEGEcSGYL--CVKGSwPGAFRTLFGDHERYEtT 583
Cdd:cd05940  227 YAATEgnSGFINFFGKPGAIGRNP-SLLRKVAPLALVKYDlESGEPIRDA-EGRCikVPRGE-PGLLISRINPLEPFD-G 302
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 584 YFKPFA--------------GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:cd05940  303 YTDPAAtekkilrdvfkkgdAWFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQ 380
PRK05691 PRK05691
peptide synthase; Validated
356-743 2.52e-12

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 70.97  E-value: 2.52e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  356 FLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYaFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVfegaPN--YP 433
Cdd:PRK05691 3873 YVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPY-LALSEADVIAQTASQSFDISVWQFLAAPLFGARVEIV----PNaiAH 3947
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  434 DPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDdkfvtRHSRKSLRVLGSVGEPINPS-AWRWF--------FNVVGDSRCP 504
Cdd:PRK05691 3948 DPQGLLAHVQAQGITVLESVPSLIQGMLAED-----RQALDGLRWMLPTGEAMPPElARQWLqrypqiglVNAYGPAECS 4022
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  505 ISDTWWQTETGGFMITPLPGAWPQKPGsatfpffgvQPVIVDEKGNEIEGECSGYLCVKGSwpGAFRTLFGDHERYETTY 584
Cdd:PRK05691 4023 DDVAFFRVDLASTRGSYLPIGSPTDNN---------RLYLLDEALELVPLGAVGELCVAGT--GVGRGYVGDPLRTALAF 4091
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  585 F-KPFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAvVGIEHEVKGQGIYAFV 659
Cdd:PRK05691 4092 VpHPFGApgerLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAA-VAVQEGVNGKHLVGYL 4170
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  660 TLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLEElgdTSTLADPSVVDQLIA 739
Cdd:PRK05691 4171 VPHQTVLAQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKALPALDIGQLQS---QAYLAPRNELEQTLA 4247

                  ....*.
gi 378548265  740 --LADV 743
Cdd:PRK05691 4248 tiWADV 4253
PRK08315 PRK08315
AMP-binding domain protein; Validated
544-725 9.49e-12

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 68.30  E-value: 9.49e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 IVDEKGNEI-----EGE--CSGYLCVKGSWPGAFRTlfgdHERYETtyfkpfAGYYFSGDGCSRDKDGYYWLTGRVDDVI 616
Cdd:PRK08315 384 IVDPETGETvprgeQGElcTRGYSVMKGYWNDPEKT----AEAIDA------DGWMHTGDLAVMDEEGYVNIVGRIKDMI 453
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 617 NVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYS-EELRKSLvlmvRNQIGAFAAPDRIHWA 695
Cdd:PRK08315 454 IRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTeEDVRDFC----RGKIAHYKIPRYIRFV 529
                        170       180       190
                 ....*....|....*....|....*....|
gi 378548265 696 PGLPKTRSGKIMRRILRKIAsrqLEELGDT 725
Cdd:PRK08315 530 DEFPMTVTGKIQKFKMREMM---IEELGLQ 556
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
198-708 1.57e-11

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 67.08  E-value: 1.57e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcn 277
Cdd:cd05914    8 LTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAIFV-- 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivdaaldqSSKDGVSVgicltydnslattrentkwqngrdvwwqdvisqyptscevewvdaedplfL 357
Cdd:cd05914   86 ---------------------SDEDDVAL--------------------------------------------------I 94
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTtgGYMIYTATTFKYAFD-YKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVV----------- 425
Cdd:cd05914   95 NYTSGTTGNSKGVMLT--YRNIVSNVDGVKEVVlLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFldkipsakiia 172
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 426 ---FEGAPNYPDPgRCWDIVDKYKVSIFytaPTLVRSLMR-------DDDKFVTRHSRKSLRVLG-------SVGEPINP 488
Cdd:cd05914  173 lafAQVTPTLGVP-VPLVIEKIFKMDII---PKLTLKKFKfklakkiNNRKIRKLAFKKVHEAFGgnikefvIGGAKINP 248
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 489 SAWRwFFNVVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGNEIEGE--CSGYLCVKGSW 566
Cdd:cd05914  249 DVEE-FLRTIG---FPYTIGYGMTETAPIISYSPPNR--IRLGSAGKVIDGVEVRIDSPDPATGEGEiiVRGPNVMKGYY 322
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 567 --PGAFRTLFgDHEryettyfkpfaGYYFSGDGCSRDKDGYYWLTGRVDDVI-NVSGHRIGTAEVESALVLHPQCAEAAV 643
Cdd:cd05914  323 knPEATAEAF-DKD-----------GWFHTGDLGKIDAEGYLYIRGRKKEMIvLSSGKNIYPEEIEAKINNMPFVLESLV 390
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 644 VgiEHEVKGQGI------YAFVTLLEGVPYSEELRKSLVLMVRNQIGAFA--APDRIHWAPgLPKTRSGKIMR 708
Cdd:cd05914  391 V--VQEKKLVALayidpdFLDVKALKQRNIIDAIKWEVRDKVNQKVPNYKkiSKVKIVKEE-FEKTPKGKIKR 460
PRK05691 PRK05691
peptide synthase; Validated
197-711 2.18e-11

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 67.89  E-value: 2.18e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTc 276
Cdd:PRK05691 2213 TLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEYPLERLHYMIEDSGIGLLLS- 2291
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  277 navkrgpktinlkaivDAALDQSS---KDGVSVGiCLTYDnslattrentkwqngrdvwwQDVISQYPTSCEVEWVDAED 353
Cdd:PRK05691 2292 ----------------DRALFEALgelPAGVARW-CLEDD--------------------AAALAAYSDAPLPFLSLPQH 2334
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  354 PLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKyAFDYKSTDV----YWCTADCGwitghSYVTYGPMLNGATVVV-FEG 428
Cdd:PRK05691 2335 QAYLIYTSGSTGKPKGVVVSHGEIAMHCQAVIE-RFGMRADDCelhfYSINFDAA-----SERLLVPLLCGARVVLrAQG 2408
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  429 ApnypdpgrcWD------IVDKYKVSIFYTAPTLVRSLMRdddKFVTRHSRKSLRVLGSVGEPINPSAWR---------W 493
Cdd:PRK05691 2409 Q---------WGaeeicqLIREQQVSILGFTPSYGSQLAQ---WLAGQGEQLPVRMCITGGEALTGEHLQrirqafapqL 2476
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  494 FFNVVGdsrcpisdtwwQTETggfMITPLPGAWPQ--KPGSATFPFFGV----QPVIVDEKGNEIEGECSGYLCVKGSwp 567
Cdd:PRK05691 2477 FFNAYG-----------PTET---VVMPLACLAPEqlEEGAASVPIGRVvgarVAYILDADLALVPQGATGELYVGGA-- 2540
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265  568 GAFRtlfGDHERYETTYFK----PFAG----YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCA 639
Cdd:PRK05691 2541 GLAQ---GYHDRPGLTAERfvadPFAAdggrLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVR 2617
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265  640 EAAVVGIEHEVKGQGIYAFVTLLEGVPYSE--ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:PRK05691 2618 EAVVLALDTPSGKQLAGYLVSAVAGQDDEAqaALREALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
PTZ00216 PTZ00216
acyl-CoA synthetase; Provisional
198-417 6.62e-10

acyl-CoA synthetase; Provisional


Pssm-ID: 240316 [Multi-domain]  Cd Length: 700  Bit Score: 62.69  E-value: 6.62e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSL--AQRIVDCKpnVILt 275
Cdd:PTZ00216 122 ITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALayALRETECK--AIV- 198
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 276 CNAvKRGPKTINLkaivdaaldqsSKDGVSVGICLTYDNSLAttrENTKWQNGRDVWWQDVI-------SQYP-TSCEve 347
Cdd:PTZ00216 199 CNG-KNVPNLLRL-----------MKSGGMPNTTIIYLDSLP---ASVDTEGCRLVAWTDVVakghsagSHHPlNIPE-- 261
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 348 wvDAEDPLFLLYTSGSTGKPKGVLHTTGgymiyTATTFKYAFDYKSTDVYWCTADcgwitGHSYVTYGPM 417
Cdd:PTZ00216 262 --NNDDLALIMYTSGTTGDPKGVMHTHG-----SLTAGILALEDRLNDLIGPPEE-----DETYCSYLPL 319
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
350-711 2.33e-09

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 60.11  E-value: 2.33e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 350 DAEDPLFLLYTSGSTGKPKGVLHTTGGYM-IYTATTFKYAFDYKSTDVYWCTAdcgwitghSYV-------TYGPMLNGA 421
Cdd:cd17648   92 NSTDLAYAIYTSGTTGKPKGVLVEHGSVVnLRTSLSERYFGRDNGDEAVLFFS--------NYVfdffveqMTLALLNGQ 163
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 422 TVVVFEGAPNYpDPGRCWDIVDKYKVSIFYTAPTLVRslMRDDDkfvtrhSRKSLRVLGSVGEPINPSAwrwFFNVVGDS 501
Cdd:cd17648  164 KLVVPPDEMRF-DPDRFYAYINREKVTYLSGTPSVLQ--QYDLA------RLPHLKRVDAAGEEFTAPV---FEKLRSRF 231
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 502 RCPISDTWWQTETGGF-MITPLPGAwPQKPGSATFPFFGV---------QPVIVDEKGN-EIEGEC--SGY-----LCVK 563
Cdd:cd17648  232 AGLIINAYGPTETTVTnHKRFFPGD-QRFDKSLGRPVRNTkcyvlndamKRVPVGAVGElYLGGDGvaRGYlnrpeLTAE 310
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 564 GSWPGAFRTlfgDHERYETTYfkpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAV 643
Cdd:cd17648  311 RFLPNPFQT---EQERARGRN----ARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAV 383
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 378548265 644 VGIEHEVKGQG-----IYAFVTLLEGVPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRIL 711
Cdd:cd17648  384 VAKEDASQAQSriqkyLVGYYLPEPGHVPESDLLSFL----RAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
198-738 4.12e-09

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 59.68  E-value: 4.12e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILtcn 277
Cdd:cd17640    6 ITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALV--- 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivdaaLDQSSKDgvsvgicltydnsLATtrentkwqngrdvwwqdvisqyptscevewvdaedplfL 357
Cdd:cd17640   83 ------------------VENDSDD-------------LAT--------------------------------------I 93
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGyMIYTATTFKYAFDYKSTDVYWCTADCgWitgHSY---VTYGPMLNGATVVvFEGAPNYPD 434
Cdd:cd17640   94 IYTSGTTGNPKGVMLTHAN-LLHQIRSLSDIVPPQPGDRFLSILPI-W---HSYersAEYFIFACGCSQA-YTSIRTLKD 167
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 435 pgrcwDIvDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLG---SVGE---PIN-----PSAWRWFFNVVGdsrC 503
Cdd:cd17640  168 -----DL-KRVKPHYIVSVPRLWESLYSGIQKQVSKSSPIKQFLFLfflSGGIfkfGISgggalPPHVDTFFEAIG---I 238
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 504 PISDTWWQTETGGFMITPLPgaWPQKPGSATFPFFGVQPVIVDEKGNEI-EGECSGYLCVKGswPGAFRTLFGDHEryET 582
Cdd:cd17640  239 EVLNGYGLTETSPVVSARRL--KCNVRGSVGRPLPGTEIKIVDPEGNVVlPPGEKGIVWVRG--PQVMKGYYKNPE--AT 312
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 583 TYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVS-GHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIyafvtl 661
Cdd:cd17640  313 SKVLDSDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIEEALMRSPFIEQIMVVGQDQKRLGALI------ 386
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 378548265 662 legVPYSEELRKSLvlmvrNQIGAFAAPDRIHWAPGLPKTR--SGKIMRRILRKIASRQLEELGDTSTLADPSVVDQLI 738
Cdd:cd17640  387 ---VPNFEELEKWA-----KESGVKLANDRSQLLASKKVLKlyKNEIKDEISNRPGFKSFEQIAPFALLEEPFIENGEM 457
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
585-720 4.13e-09

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 59.62  E-value: 4.13e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 585 FKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEG 664
Cdd:PRK07445 319 ILDSQGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDP 398
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 665 VPYSEELRKSLvlmvRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASRQLE 720
Cdd:PRK07445 399 SISLEELKTAI----KDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQIAVQRLG 450
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
198-673 4.38e-09

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 59.54  E-value: 4.38e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 198 LTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTcn 277
Cdd:cd17639    6 MSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIHSLNETECSAIFT-- 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 278 avkrgpktinlkaivdaaldqsskdgvsvgicltyDNSlattrentkwqngrdvwwqdvisqyptscevewvdAEDPLFL 357
Cdd:cd17639   84 -----------------------------------DGK-----------------------------------PDDLACI 93
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 358 LYTSGSTGKPKGVLHTTGGyMIYTATTFkyafdykstdvywctadCGWITGH-----SYVTYGP-------------MLN 419
Cdd:cd17639   94 MYTSGSTGNPKGVMLTHGN-LVAGIAGL-----------------GDRVPELlgpddRYLAYLPlahifelaaenvcLYR 155
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 420 GATV-------------------------VVFEGAPnypdpgRCWDIVDK-----------YKVSIFYTAPTLVRSLMRD 463
Cdd:cd17639  156 GGTIgygsprtltdkskrgckgdltefkpTLMVGVP------AIWDTIRKgvlaklnpmggLKRTLFWTAYQSKLKALKE 229
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 464 D------DKFVTRHSRKS----LRVLGSVGEPINPSAWRwFFNVVGdsrCPISDTWWQTETGGFMITPLPGAWpqKPGSA 533
Cdd:cd17639  230 GpgtpllDELVFKKVRAAlggrLRYMLSGGAPLSADTQE-FLNIVL---CPVIQGYGLTETCAGGTVQDPGDL--ETGRV 303
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 534 TFPFFGVQPVIVD-EKGNEI--EGECSGYLCVKGswPGAFRTLFGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTG 610
Cdd:cd17639  304 GPPLPCCEIKLVDwEEGGYStdKPPPRGEILIRG--PNVFKGYYKNPEKTKEAFDG--DGWFHTGDIGEFHPDGTLKIID 379
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 378548265 611 RVDD-VINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKgqgIYAFVtllegVPYSEELRK 673
Cdd:cd17639  380 RKKDlVKLQNGEYIALEKLESIYRSNPLVNNICVYADPDKSY---PVAIV-----VPNEKHLTK 435
PRK09274 PRK09274
peptide synthase; Provisional
181-646 4.78e-08

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 56.45  E-value: 4.78e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAI----HWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGD-AVVIYLPMLmELPIAMLACARIGAVHSVVFAG 255
Cdd:PRK09274  21 DQLAVavpgGRGADGKLAYDELSFAELDARSDAIAHGLNAAGIGRGMrAVLMVTPSL-EFFALTFALFKAGAVPVLVDPG 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 256 FSADSLAQRIVDCKPNVILTCnavkrgPKTINLKAIVDAALDqSSKDGVSVG-ICLTYDNSLATTRENTKwqngrdvwwq 334
Cdd:PRK09274 100 MGIKNLKQCLAEAQPDAFIGI------PKAHLARRLFGWGKP-SVRRLVTVGgRLLWGGTTLATLLRDGA---------- 162
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 335 dvisqyPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGgymIYTA--TTFKYAFDYKSTDVYWCTADCgwitghsYV 412
Cdd:PRK09274 163 ------AAPFPMADLAPDDMAAILFTSGSTGTPKGVVYTHG---MFEAqiEALREDYGIEPGEIDLPTFPL-------FA 226
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 413 TYGPMLnGATVVVFEGAPNYP---DPGRCWDIVDKYKVSIFYTAPTLVRSLmrdddkfvTRHSRKSLRVLGSV------G 483
Cdd:PRK09274 227 LFGPAL-GMTSVIPDMDPTRPatvDPAKLFAAIERYGVTNLFGSPALLERL--------GRYGEANGIKLPSLrrvisaG 297
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 484 EPINPSAWRWF----------FNVVGDSRC-PIS---------DTWWQTETGGfmitplpGAWPQKPgsatfpffgVQPV 543
Cdd:PRK09274 298 APVPIAVIERFramlppdaeiLTPYGATEAlPISsiesreilfATRAATDNGA-------GICVGRP---------VDGV 361
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 544 ------IVDEKGNEIEG--ECS----GYLCVKGswP----------------------GAFRTLFGDheryettyfkpfA 589
Cdd:PRK09274 362 evriiaISDAPIPEWDDalRLAtgeiGEIVVAG--PmvtrsyynrpeatrlakipdgqGDVWHRMGD------------L 427
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 378548265 590 GYyfsgdgcsRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGI 646
Cdd:PRK09274 428 GY--------LDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALVGV 476
PRK05850 PRK05850
acyl-CoA synthetase; Validated
183-370 8.52e-08

acyl-CoA synthetase; Validated


Pssm-ID: 235624 [Multi-domain]  Cd Length: 578  Bit Score: 55.72  E-value: 8.52e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 183 TAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVkKGDAVVIYLPMLMELPIAMLA--CARIGAVH-SVVFAGFSAD 259
Cdd:PRK05850  21 TFIDYEQDPAGVAETLTWSQLYRRTLNVAEELRRHGS-TGDRAVILAPQGLEYIVAFLGalQAGLIAVPlSVPQGGAHDE 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 260 SLAQRIVDCKPNVILTCNAVkrgpktinlkaIVDAALDQSSKDGVSVGICLTYDnSLattrentkwqngrdvwwqDVISq 339
Cdd:PRK05850 100 RVSAVLRDTSPSVVLTTSAV-----------VDDVTEYVAPQPGQSAPPVIEVD-LL------------------DLDS- 148
                        170       180       190
                 ....*....|....*....|....*....|.
gi 378548265 340 yPTSCEVEWVDAEDPLFLLYTSGSTGKPKGV 370
Cdd:PRK05850 149 -PRGSDARPRDLPSTAYLQYTSGSTRTPAGV 178
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
351-646 2.52e-07

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 54.00  E-value: 2.52e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 AEDPLFLLYTSGSTGKPKGVLhttggymiYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLnGATVVVFEGAP 430
Cdd:cd05910   84 ADEPAAILFTSGSTGTPKGVV--------YRHGTFAAQIDALRQLYGIRPGEVDLATFPLFALFGPAL-GLTSVIPDMDP 154
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 431 NYP---DPGRCWDIVDKYKVSIFYTAPTLVRSLMRdddkFVTRHSRK--SLRVLGSVGEPINP-SAWRW---------FF 495
Cdd:cd05910  155 TRParaDPQKLVGAIRQYGVSIVFGSPALLERVAR----YCAQHGITlpSLRRVLSAGAPVPIaLAARLrkmlsdeaeIL 230
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 496 NVVGDSRC-PIS---DTWWQTETggfmiTPLPgawpqKPGSAT---FPFFGVQPVIV---DEKGNEIEGECS------GY 559
Cdd:cd05910  231 TPYGATEAlPVSsigSRELLATT-----TAAT-----SGGAGTcvgRPIPGVRVRIIeidDEPIAEWDDTLElprgeiGE 300
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 560 LCVKGswPGAFRTLfgdHERYETTYFK--PFAGYYF---SGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVL 634
Cdd:cd05910  301 ITVTG--PTVTPTY---VNRPVATALAkiDDNSEGFwhrMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNT 375
                        330
                 ....*....|..
gi 378548265 635 HPQCAEAAVVGI 646
Cdd:cd05910  376 HPGVRRSALVGV 387
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
199-719 4.67e-07

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 53.08  E-value: 4.67e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 199 TYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMEL-PIAMLACARIGAV-------HSVVFAGFSADSLAqrivdckp 270
Cdd:PRK07768  31 TWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIaPTAQGLWMRGASLtmlhqptPRTDLAVWAEDTLR-------- 102
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 271 nvilTCNAVkrGPKTINLKAIVDAALDQSSKDGVSVgicLTYDNSLAttrentkwqngrdvwwqdvisqyPTSCEVEWVD 350
Cdd:PRK07768 103 ----VIGMI--GAKAVVVGEPFLAAAPVLEEKGIRV---LTVADLLA-----------------------ADPIDPVETG 150
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 351 AEDPLFLLYTSGSTGKPKGVLHTTG-----GYMIYTATTFKYAFDYKstdVYW--CTADCGWItghSYVTYgPMLNGATV 423
Cdd:PRK07768 151 EDDLALMQLTSGSTGSPKAVQITHGnlyanAEAMFVAAEFDVETDVM---VSWlpLFHDMGMV---GFLTV-PMYFGAEL 223
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 424 VVFEGAPNYPDPGRCWDIVDKYKVSIfYTAPT-----LVRSLMRDDDKfvTRHSRKSLRVLGSVGEPINPSAWRWFFNVV 498
Cdd:PRK07768 224 VKVTPMDFLRDPLLWAELISKYRGTM-TAAPNfayalLARRLRRQAKP--GAFDLSSLRFALNGAEPIDPADVEDLLDAG 300
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 499 G----DSRCPISDTWWQTETGGFMITPLPG-----------------AWPQKPGSAT------FPFFGVQPVIVDEKGN- 550
Cdd:PRK07768 301 ArfglRPEAILPAYGMAEATLAVSFSPCGAglvvdevdadllaalrrAVPATKGNTRrlatlgPPLPGLEVRVVDEDGQv 380
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 551 ---------EIEGEC--SGYLCVKGswpgaFRTLFGDHeryettyfkpfaGYYFSGDGCSRDKDGYYWLTGRVDDVINVS 619
Cdd:PRK07768 381 lpprgvgviELRGESvtPGYLTMDG-----FIPAQDAD------------GWLDTGDLGYLTEEGEVVVCGRVKDVIIMA 443
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 620 GHRIGTAEVESALV----LHPQCAEAavVGIEHEVKGQGiyaFVTLLEGVPYSEE-----LRKSLVLMVRNQIGafAAPD 690
Cdd:PRK07768 444 GRNIYPTDIERAAArvegVRPGNAVA--VRLDAGHSREG---FAVAVESNAFEDPaevrrIRHQVAHEVVAEVG--VRPR 516
                        570       580       590
                 ....*....|....*....|....*....|.
gi 378548265 691 RIH-WAPG-LPKTRSGKimrriLRKIASRQL 719
Cdd:PRK07768 517 NVVvLGPGsIPKTPSGK-----LRRANAAEL 542
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
181-370 6.06e-07

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 52.59  E-value: 6.06e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 181 DKTAIHWEGnelgvdASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGavHSVVFAGFSadS 260
Cdd:PRK04813  17 DFPAYDYLG------EKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAG--HAYIPVDVS--S 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 261 LAQRIVD----CKPNVILtcnavkrgpktinlkAIVDAALDQsskDGVSVgicLTYDNSlattrentkwqngrdvwwQDV 336
Cdd:PRK04813  87 PAERIEMiievAKPSLII---------------ATEELPLEI---LGIPV---ITLDEL------------------KDI 127
                        170       180       190
                 ....*....|....*....|....*....|....
gi 378548265 337 ISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGV 370
Cdd:PRK04813 128 FATGNPYDFDHAVKGDDNYYIIFTSGTTGKPKGV 161
PRK08308 PRK08308
acyl-CoA synthetase; Validated
593-727 8.57e-06

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 48.88  E-value: 8.57e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 593 FSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPySEELR 672
Cdd:PRK08308 294 FTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHEEID-PVQLR 372
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 378548265 673 KslvlMVRNQIGAFAAPDRIHWAPGLPKTRSGKImrrilrkiaSRQLEELGDTST 727
Cdd:PRK08308 373 E----WCIQHLAPYQVPHEIESVTEIPKNANGKV---------SRKLLELGEVTA 414
PRK09188 PRK09188
serine/threonine protein kinase; Provisional
631-742 4.51e-05

serine/threonine protein kinase; Provisional


Pssm-ID: 236400 [Multi-domain]  Cd Length: 365  Bit Score: 46.30  E-value: 4.51e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 631 ALVLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVpYSEELRKSLVlmvrnQIGAFAAPDRIHWAPGLPKTRSGKIMRRI 710
Cdd:PRK09188 248 ALKSDPAVSDVAIALFSLPAKGVGLYAFVEAELPA-DEKSLRARLA-----GAKPPKPPEHIQPVAALPRDADGTVRDDI 321
                         90       100       110
                 ....*....|....*....|....*....|..
gi 378548265 711 LRKIASRQLEELGDtsTLADPSVVDQLIALAD 742
Cdd:PRK09188 322 LRLIAMNQIDELDD--LLREPEIRGLVEAIAA 351
alpha_am_amid TIGR03443
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ...
197-371 7.03e-05

L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.


Pssm-ID: 274582 [Multi-domain]  Cd Length: 1389  Bit Score: 46.60  E-value: 7.03e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265   197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGF-------------------- 256
Cdd:TIGR03443  270 SFTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVIDPAYpparqtiylsvakpralivi 349
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265   257 -SADSLAQRIVDCKPN---VILTCNAVkrgpKTINLKAIVDAALDQSSKDgvsvgiCLTYDNSLATTRENtkwqngrdvw 332
Cdd:TIGR03443  350 eKAGTLDQLVRDYIDKeleLRTEIPAL----ALQDDGSLVGGSLEGGETD------VLAPYQALKDTPTG---------- 409
                          170       180       190
                   ....*....|....*....|....*....|....*....
gi 378548265   333 wqdVIsqyptscevewVDAEDPLFLLYTSGSTGKPKGVL 371
Cdd:TIGR03443  410 ---VV-----------VGPDSNPTLSFTSGSEGIPKGVL 434
A_NRPS_alphaAR cd17647
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ...
197-252 3.48e-04

Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.


Pssm-ID: 341302 [Multi-domain]  Cd Length: 520  Bit Score: 44.05  E-value: 3.48e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 197 SLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVV 252
Cdd:cd17647   20 SFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVI 75
PRK06334 PRK06334
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
332-480 1.25e-03

long chain fatty acid--[acyl-carrier-protein] ligase; Validated


Pssm-ID: 180533 [Multi-domain]  Cd Length: 539  Bit Score: 42.11  E-value: 1.25e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 332 WWQDVISQYPTSCEVEWV---------DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYaFDYKSTDVYWCTAD 402
Cdd:PRK06334 154 FWEKCRIGIYMSIPFEWLmrwfgvsdkDPEDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKF-FSPKEDDVMMSFLP 232
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 378548265 403 CGWITGHSYVTYGPMLNGATvVVFEGAPNYPDpgRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLG 480
Cdd:PRK06334 233 PFHAYGFNSCTLFPLLSGVP-VVFAYNPLYPK--KIVEMIDEAKVTFLGSTPVFFDYILKTAKKQESCLPSLRFVVIG 307
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
359-397 5.92e-03

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 39.89  E-value: 5.92e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 378548265 359 YTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKS---TDVY 397
Cdd:cd05927  121 YTSGTTGNPKGVMLTHGNIVSNVAGVFKILEILNKinpTDVY 162
PRK07868 PRK07868
acyl-CoA synthetase; Validated
568-703 7.73e-03

acyl-CoA synthetase; Validated


Pssm-ID: 236121 [Multi-domain]  Cd Length: 994  Bit Score: 39.70  E-value: 7.73e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 378548265 568 GAFRTLFGDHERYETTyfkpfaGYYFSgdgcsRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIE 647
Cdd:PRK07868 825 SVKRGVFAPADTWIST------EYLFR-----RDDDGDYWLVDRRGSVIRTARGPVYTEPVTDALGRIGGVDLAVTYGVE 893
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 378548265 648 HEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQigafaAPDRIHWAPGLPKTRS 703
Cdd:PRK07868 894 VGGRQLAVAAVTLRPGAAITAADLTEALASLPVGL-----GPDIVHVVPEIPLSAT 944
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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