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Conserved domains on  [gi|991865715|gb|AMG33984|]
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iron-sulfur cluster assembly accessory protein [Staphylococcus saprophyticus]

Protein Classification

HesB/IscA family protein( domain architecture ID 10001059)

HesB/IscA family protein is a scaffold protein upon which 2Fe-2S clusters are assembled and subsequently transferred to acceptor proteins; similar to iron-sulfur assembly protein IscA that is involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway

Gene Ontology:  GO:0016226|GO:0051536
PubMed:  32108236

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 3-109 3.15e-50

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 154.54  E-value: 3.15e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   3 VINMTESAAYEVKDMLQQNDMPDGYLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFK 82
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                         90       100
                 ....*....|....*....|....*..
gi 991865715  83 QSLMGGGFQIDNPNAIAACGCGSSFRT 109
Cdd:COG0316   81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 3-109 3.15e-50

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 154.54  E-value: 3.15e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   3 VINMTESAAYEVKDMLQQNDMPDGYLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFK 82
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                         90       100
                 ....*....|....*....|....*..
gi 991865715  83 QSLMGGGFQIDNPNAIAACGCGSSFRT 109
Cdd:COG0316   81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 7-109 7.66e-40

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 128.47  E-value: 7.66e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715    7 TESAAYEVKDMLQQNDMPDGYLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFKQSLM 86
Cdd:TIGR00049   3 TDSAAKRIKALLAGEGEPNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEELL 82

                          90       100
                  ....*....|....*....|...
gi 991865715   87 GGGFQIDNPNAIAACGCGSSFRT 109
Cdd:TIGR00049  83 GSGFTFTNPNAKGTCGCGKSFSV 105
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 4-107 3.94e-26

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 93.84  E-value: 3.94e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   4 INMTESAAYEVKDMLQQNDMPDGYLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFKQ 83
Cdd:PRK13623  10 LVFTDAAAAKVKELIEEEGNPDLKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDYTE 89

                         90       100
                 ....*....|....*....|....
gi 991865715  84 SLMGGGFQIDNPNAIAACGCGSSF 107
Cdd:PRK13623  90 GLEGSRFVIKNPNAKTTCGCGSSF 113
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 4-104 3.68e-15

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 65.75  E-value: 3.68e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715    4 INMTESAAYEVKDMLQQNDmpdGYLKVKVNGGG-------CT-GLTYGM-SAEEEPGENDEIFEFYGLKVLVDKYDKPVL 74
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDK---KELRLDVDDGGgpyskggCSiGGKFSLvLVDEPDPDYDEVIESNGGPIYVDSYSLPFL 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 991865715   75 D-GTTIDFKQSLMGGGFQIDNPNAIAACGCG 104
Cdd:pfam01521  80 DeGLTLDFVEDLGTLGLKSDNGNLDGNVGCG 110
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 4-105 1.23e-11

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 56.57  E-value: 1.23e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   4 INMTESAAYEVKDMLQQN--DMPdgyLKVKVNGGGCTGLTYGMsAEEEPGENDEIFEFYGLKVLVDK----YDKPVldgt 77
Cdd:NF038090   2 LEVTDLASRKLKEYFADKkiDSP---IRVFLNQGGUAGPSLGL-ALDEPKENDEVFEQDGLTFVIDKelleQCGPI---- 73

                         90       100
                 ....*....|....*....|....*...
gi 991865715  78 TIDFkqSLMGGGFQIDNPNAIAACGCGS 105
Cdd:NF038090  74 KVDF--IDCRSGFSITSSMPLGGGGCGS 99
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 3-109 3.15e-50

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 154.54  E-value: 3.15e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   3 VINMTESAAYEVKDMLQQNDMPDGYLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFK 82
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                         90       100
                 ....*....|....*....|....*..
gi 991865715  83 QSLMGGGFQIDNPNAIAACGCGSSFRT 109
Cdd:COG0316   81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 7-109 7.66e-40

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 128.47  E-value: 7.66e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715    7 TESAAYEVKDMLQQNDMPDGYLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFKQSLM 86
Cdd:TIGR00049   3 TDSAAKRIKALLAGEGEPNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEELL 82

                          90       100
                  ....*....|....*....|...
gi 991865715   87 GGGFQIDNPNAIAACGCGSSFRT 109
Cdd:TIGR00049  83 GSGFTFTNPNAKGTCGCGKSFSV 105
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 4-107 3.94e-26

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 93.84  E-value: 3.94e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   4 INMTESAAYEVKDMLQQNDMPDGYLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFKQ 83
Cdd:PRK13623  10 LVFTDAAAAKVKELIEEEGNPDLKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDYTE 89

                         90       100
                 ....*....|....*....|....
gi 991865715  84 SLMGGGFQIDNPNAIAACGCGSSF 107
Cdd:PRK13623  90 GLEGSRFVIKNPNAKTTCGCGSSF 113
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 2-107 2.05e-22

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 84.11  E-value: 2.05e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715    2 TVINMTESAAYEVKDMLQQNDMPDGyLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDF 81
Cdd:TIGR01997   1 AVITLTDAAAIHIRELVAKRPEAVG-IRLGVKKTGCAGMEYVLDLVSEPKKDDDLIEHDGAKVFVAPEAVLFILGTQVDF 79

                          90       100
                  ....*....|....*....|....*.
gi 991865715   82 KQSLMGGGFQIDNPNAIAACGCGSSF 107
Cdd:TIGR01997  80 VRTTLRQGFKFNNPNATSACGCGESF 105
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 4-107 7.88e-19

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 75.25  E-value: 7.88e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715    4 INMTESAAYEVKDMLQQNDMPDGyLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFKQ 83
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANRGKGFG-LRLGVKTSGCSGMAYVLEFVDEPTPDDIVFEDKGVKIVIDGKSLQYLDGTQLDFVK 79

                          90       100
                  ....*....|....*....|....
gi 991865715   84 SLMGGGFQIDNPNAIAACGCGSSF 107
Cdd:TIGR02011  80 EGLNEGFKFTNPNVKDECGCGESF 103
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
4-107 5.79e-18

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 72.98  E-value: 5.79e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   4 INMTESAAYEVKDMLQQNDMPDGyLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFKQ 83
Cdd:PRK09502   3 ITLSDSAAARVNTFLANRGKGFG-LRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVK 81

                         90       100
                 ....*....|....*....|....
gi 991865715  84 SLMGGGFQIDNPNAIAACGCGSSF 107
Cdd:PRK09502  82 EGLNEGFKFTNPNVKDECGCGESF 105
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 4-107 7.38e-17

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 70.54  E-value: 7.38e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   4 INMTESAAYEVKDMLQQNDMPDGyLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTIDFKQ 83
Cdd:PRK09504  18 LTLTPAAAAHIRELMAKQPGMKG-VRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYVR 96

                         90       100
                 ....*....|....*....|....
gi 991865715  84 SLMGGGFQIDNPNAIAACGCGSSF 107
Cdd:PRK09504  97 EGLNQIFKFHNPKAQNECGCGESF 120
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 3-107 1.12e-16

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 71.11  E-value: 1.12e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   3 VINMTESAAYEVKDmLQQND--MPDGYLKVKVNGGGCTGLTYGMSAEEEPGENDEIFEFYGLKVLVDKYDKPVLDGTTID 80
Cdd:PLN03082  54 AVHMTDNCIRRLKE-LQTSEpsAEDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVKLVVDNISYDFVKGATVD 132

                         90       100
                 ....*....|....*....|....*...
gi 991865715  81 FKQSLMGGGFQI-DNPNAIAACGCGSSF 107
Cdd:PLN03082 133 YVEELIRSAFVVsTNPSAVGGCSCKSSF 160
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 4-104 3.68e-15

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 65.75  E-value: 3.68e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715    4 INMTESAAYEVKDMLQQNDmpdGYLKVKVNGGG-------CT-GLTYGM-SAEEEPGENDEIFEFYGLKVLVDKYDKPVL 74
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDK---KELRLDVDDGGgpyskggCSiGGKFSLvLVDEPDPDYDEVIESNGGPIYVDSYSLPFL 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 991865715   75 D-GTTIDFKQSLMGGGFQIDNPNAIAACGCG 104
Cdd:pfam01521  80 DeGLTLDFVEDLGTLGLKSDNGNLDGNVGCG 110
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 4-105 1.23e-11

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 56.57  E-value: 1.23e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 991865715   4 INMTESAAYEVKDMLQQN--DMPdgyLKVKVNGGGCTGLTYGMsAEEEPGENDEIFEFYGLKVLVDK----YDKPVldgt 77
Cdd:NF038090   2 LEVTDLASRKLKEYFADKkiDSP---IRVFLNQGGUAGPSLGL-ALDEPKENDEVFEQDGLTFVIDKelleQCGPI---- 73

                         90       100
                 ....*....|....*....|....*...
gi 991865715  78 TIDFkqSLMGGGFQIDNPNAIAACGCGS 105
Cdd:NF038090  74 KVDF--IDCRSGFSITSSMPLGGGGCGS 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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