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Conserved domains on  [gi|641744967|gb|AIA70438|]
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hypothetical protein EV46_07525 [Pectobacterium atrosepticum]

Protein Classification

non-ribosomal peptide synthetase( domain architecture ID 13482993)

non-ribosomal peptide synthetase is a modular multidomain enzyme that acts as an assembly line to catalyze the biosynthesis of complex natural products; similar to Bacillus subtilis dimodular nonribosomal peptide synthase

EC:  6.2.1.-
Gene Ontology:  GO:0031177|GO:1901576|GO:0016874
PubMed:  20153164|28526268|26604034

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
PRK12467 super family cl36129
peptide synthase; Provisional
 3-2325 0e+00

peptide synthase; Provisional


The actual alignment was detected with superfamily member PRK12467:

Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 1873.31  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    3 LVTLSQEEIDTVIATVPDGVANVQDIyPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDIL 82
Cdd:PRK12467   25 LEKMQEEGVSFANLPIPQVRSAFERI-PLSYAQERQWFLWQLDPDSAAYNIPTALRLRGELDVSALRRAFDALVARHESL 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   83 RTAICWQGlHQPVQVVWRQAPLTV--NTLTTTSSDTVPAQLRAATDPSNHRL-NLSNAPLLSAT---TAHDpvcgEWLLS 156
Cdd:PRK12467  104 RTRFVQDE-EGFRQVIDASLSLTIplDDLANEQGRARESQIEAYINEEVARPfDLANGPLLRVRllrLADD----EHVLV 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  157 LSIHHLISDHITQALIIDEIRLLL----EDRPEALPK-PLPYRNF-IAQILSVPLSEHEQ---YFRNRLADIDTPTA-PF 226
Cdd:PRK12467  179 VTLHHIISDGWSMRVLVEELVQLYsaysQGREPSLPAlPIQYADYaIWQRSWLEAGERERqlaYWQEQLGGEHTVLElPT 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  227 DLVDVQGNGEDITEARLSLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLgaDRVMG 306
Cdd:PRK12467  259 DRPRPAVPSYRGARLRVDLPQALSAGLKALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANRNRVET--ERLIG 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  307 MFINTLPLRVSLRER-SVHDVVQATSHELMMLLAHEQAP---LALAQQCSQVPPPLPLFSTLFNYRHSQKDASSQFWEGM 382
Cdd:PRK12467  337 FFVNTQVLKAEVDPQaSFLELLQQVKRTALGAQAHQDLPfeqLVEALQPERSLSHSPLFQVMFNHQNTATGGRDREGAQL 416

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  383 RQLS------GRERTNYPITLSVDDLGDGFNLTAKTVMGVDPERIVHYMLTAIENLVTSLEKTPQQPALSQPILPKSERQ 456
Cdd:PRK12467  417 PGLTveelswARHTAQFDLALDTYESAQGLWAAFTYATDLFEATTIERLATHWRNLLEAIVAEPRRRLGELPLLDAEERA 496

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  457 QVLVDFNATDADFPREMlIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEM 536
Cdd:PRK12467  497 RELVRWNAPATEYAPDC-VHQLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEM 575

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  537 MVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLN--STLPTVLLDTPAA--AACPDTNPVVQgLH 612
Cdd:PRK12467  576 VVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQSHLLAQLPvpAGLRSLCLDEPADllCGYSGHNPEVA-LD 654

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  613 AAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRA 691
Cdd:PRK12467  655 PDNLAYVIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTElFGALASGATLHLLPPDCAR 734

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  692 DAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLgSDPAYQPAQVLIGGEAISPAVWSRLQSLSD-TRFINVYGPTECTVDA 770
Cdd:PRK12467  735 DAEAFAALMADQGVTVLKIVPSHLQALLQASR-VALPRPQRALVCGGEALQVDLLARVRALGPgARLINHYGPTETTVGV 813

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  771 TACVV---DRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSADpAARIY 847
Cdd:PRK12467  814 STYELsdeERDFGNVPIGQPLANLGLYILDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFGAD-GGRLY 892

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  848 KTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIArEDSPGDTRLVAYL--CARPDAELHPA 925
Cdd:PRK12467  893 RTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLA-QPGDAGLQLVAYLvpAAVADGAEHQA 971

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  926 ---ALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAfATRDYEAPQGGIETALAALWQELLGLDRVGR 1002
Cdd:PRK12467  972 trdELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPKPDASA-VQATFVAPQTELEKRLAAIWADVLKVERVGL 1050

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1003 HDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAVTATAHDAPFTLPVADRTQPLPLSFSQQRLWFLA 1081
Cdd:PRK12467 1051 TDNFFELGGHSLLATQVISRVRQRlGIQVPLRTLFEHQTLAGFAQAVAAQQQGAQPALPDVDRDQPLPLSYAQERQWFLW 1130

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1082 QLDPaASQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPD-TLGFSLSSHDLRKLDEAART 1160
Cdd:PRK12467 1131 QLEP-GSAAYHIPQALRLKGPLDIEALERSFDALVARHESLRTTFVQEDGRTRQVIHPVgSLTLEEPLLLAADKDEAQLK 1209

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1161 TRVaelaEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQY 1240
Cdd:PRK12467 1210 VYV----EAEARQPFDLEQGPLLRVGLLRLAADEHVLVLTLHHIVSDGWSMQVLVDELVALYAAYSQGQSLQLPALPIQY 1285

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1241 ADYAVWQRQWLQ-GETLNDLRdYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHALNRQQGTTLFMT 1319
Cdd:PRK12467 1286 ADYAVWQRQWMDaGERARQLA-YWKAQLGGEQPVLELPTDRPRPAVQSHRGARLAFELPPALAEGLRALARREGVTLFML 1364

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1320 LLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALDAYAHQALPFEQV 1399
Cdd:PRK12467 1365 LLASFQTLLHRYSGQDDIRVGVPIANRNRAETEGLIGFFVNTQVLRAEVDGQASFQQLLQQVKQAALEAQAHQDLPFEQL 1444

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1400 VEILQPARSLSYSPIFQVMLSLNNTPAQAL-TLPDLTLSAVERPQHSTHFDLSLSLIETENGLNGGLVYATDLFDRETIL 1478
Cdd:PRK12467 1445 VEALQPERSLSHSPLFQVMFNHQRDDHQAQaQLPGLSVESLSWESQTAQFDLTLDTYESSEGLQASLTYATDLFEASTIE 1524

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1479 RVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDA 1558
Cdd:PRK12467 1525 RLAGHWLNLLQGLVADPERRLGELDLLDEAERRQILEGWNATHTGYPLARLVHQLIEDQAAATPEAVALVFGEQELTYGE 1604

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1559 LNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQAN--QR 1636
Cdd:PRK12467 1605 LNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSHlqAR 1684

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1637 ALLTGDVPRILLDTAD--FSHLSEDNPHVpGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVL 1714
Cdd:PRK12467 1685 LPLPDGLRSLVLDQEDdwLEGYSDSNPAV-NLAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVL 1763

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1715 QKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSG 1794
Cdd:PRK12467 1764 QFTSFAFDVSVWELFWPLINGARLVIAPPGAHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVEHPLSLRRVVCGG 1843

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1795 EALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDD--HRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHI 1871
Cdd:PRK12467 1844 EALEVEALRPWLERLpDTGLFNLYGPTETAVDVTHWTCRRKDleGRDSVPIGQPIANLSTYILDASLNPVPIGVAGELYL 1923

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1872 GGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGV 1951
Cdd:PRK12467 1924 GGVGLARGYLNRPALTAERFVADPF-GTVGSRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGV 2002

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1952 QEAVVVArEDSPGDTRLVAYLCPQ----PGVTPDPADLRQQLGQHLA----EYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK12467 2003 REAVVIA-QDGANGKQLVAYVVPTdpglVDDDEAQVALRAILKNHLKaslpEYMVPAHLVFLARMPLTPNGKLDRKALPA 2081

                        2090      2100      2110      2120      2130      2140      2150      2160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2024 PDQSAVaTRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGLALDVRGVFSTPVLSDMA 2103
Cdd:PRK12467 2082 PDASEL-QQAYVAPQSELEQRLAAIWQDVLGLEQVGLHDNFFELGGDSIISIQVVSRARQAGIRFTPKDLFQHQTVQSLA 2160

                        2170      2180      2190      2200      2210      2220      2230      2240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2104 qAILAHQDKPAVvvppnripADSTAITPDLlplvtltqpeidritdtvsggasniqdiyPLAPLQEgiLF--------HH 2175
Cdd:PRK12467 2161 -AVAQEGDGTVS--------IDQGPVTGDL-----------------------------PLLPIQQ--MFfaddiperHH 2200

                        2250      2260      2270      2280      2290      2300      2310      2320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2176 LLQEqgdtyllrsmVAFTHRERLDAFL--SALQQVIDRHDILRTAVCWQDLSqpvqvvWRQAIL-PINHFEP---TSPED 2249
Cdd:PRK12467 2201 WNQS----------VLLEPREALDAELleAALQALLVHHDALRLGFVQEDGG------WSAMHRaPEQERRPllwQVVVA 2264

                        2330      2340      2350      2360      2370      2380      2390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 2250 VLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLaLSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLP 2325
Cdd:PRK12467 2265 DKEELEALCEQAQRSLDLEEGPLLRAVLATLPDGSQRLL-LVIHHLVVDGVSWRILLEDLQTAYRQLQGGQPVKLP 2339
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 2159-3191 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


:

Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 868.40  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2159 QDIYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVC--WQDLSQPVQVVWRQAI 2236
Cdd:COG1020    15 AAPLPLSAAQQRLWLLLLLLLGSAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRtrAGRPVQVIQPVVAAPL 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2237 LPINHFEPTSPEDVLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQnEWLLALSFHHLISDHMTLALIVGEIRLLLQHQ 2316
Cdd:COG1020    95 PVVVLLVDLEALAEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLLL-LLLLLLALHHIISDGLSDGLLLAELLRLYLAA 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2317 ADALPTPLP---------YRNFIAQTLSVPNSAHEAYFRDKLA-DVDEPTAPFGLLNVQGSGGDIHEARLVLDATLASAI 2386
Cdd:COG1020   174 YAGAPLPLPplpiqyadyALWQREWLQGEELARQLAYWRQQLAgLPPLLELPTDRPRPAVQSYRGARVSFRLPAELTAAL 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2387 RQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISL-ADRGAAEVVERTSH 2465
Cdd:COG1020   254 RALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGR--PRPELEGLVGFFVNTLPLRVDLsGDPSFAELLARVRE 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2466 DLMTLLEHEQAPLALAQR---CSGVAPPMPLFSTLLNYRHTQASSTDNTLSDIRVLT-SEERTNYPLTLAVDDRGEGFSL 2541
Cdd:COG1020   332 TLLAAYAHQDLPFERLVEelqPERDLSRNPLFQVMFVLQNAPADELELPGLTLEPLElDSGTAKFDLTLTVVETGDGLRL 411

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2542 VAQTLEDIDPHRLLNYLMTAISSLVDALETEPQRSILNLPVLPDSERQQMLVDFNATDADIPRHALIHELFEAQVACTPD 2621
Cdd:COG1020   412 TLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQLLAEWNATAAPYPADATLHELFEAQAARTPD 491

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2622 AIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDD 2701
Cdd:COG1020   492 AVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAERLAYMLED 571

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2702 AKPVALISQSAHLG-IMNGSLPVILLDDGEtrpFDNEPDTPLDArkqGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNF 2780
Cdd:COG1020   572 AGARLVLTQSALAArLPELGVPVLALDALA---LAAEPATNPPV---PVTPDDLAYVIYTSGSTGRPKGVMVEHRALVNL 645

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2781 LCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVEL 2860
Cdd:COG1020   646 LAWMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLTPSLLRALLDA 725

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2861 RDFALPPGFKALCGGEALPENLATALLQKV--TTLWNLYGPTETTIWSTLNGLTTPTPY-----IGHPIANTQIYILDAQ 2933
Cdd:COG1020   726 APEALPSLRLVLVGGEALPPELVRRWRARLpgARLVNLYGPTETTVDSTYYEVTPPDADggsvpIGRPIANTRVYVLDAH 805

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2934 GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIE 3013
Cdd:COG1020   806 LQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPF-GFPGARLYRTGDLARWLPDGNLEFLGRADDQVKIRGFRIE 884

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3014 LGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLD 3093
Cdd:COG1020   885 LGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLD 964

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3094 RKALPAPDQSAMATrgYEAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQH 3173
Cdd:COG1020   965 RLALPAPAAAAAAA--AAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAARLLLLLLLLLLLFLA 1042

                        1050
                  ....*....|....*...
gi 641744967 3174 PQLSALAEVILAAQIHAA 3191
Cdd:COG1020  1043 AAAAAAAAAAAAAAAAAA 1060
 
Name Accession Description Interval E-value
PRK12467 PRK12467
peptide synthase; Provisional
 3-2325 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 1873.31  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    3 LVTLSQEEIDTVIATVPDGVANVQDIyPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDIL 82
Cdd:PRK12467   25 LEKMQEEGVSFANLPIPQVRSAFERI-PLSYAQERQWFLWQLDPDSAAYNIPTALRLRGELDVSALRRAFDALVARHESL 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   83 RTAICWQGlHQPVQVVWRQAPLTV--NTLTTTSSDTVPAQLRAATDPSNHRL-NLSNAPLLSAT---TAHDpvcgEWLLS 156
Cdd:PRK12467  104 RTRFVQDE-EGFRQVIDASLSLTIplDDLANEQGRARESQIEAYINEEVARPfDLANGPLLRVRllrLADD----EHVLV 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  157 LSIHHLISDHITQALIIDEIRLLL----EDRPEALPK-PLPYRNF-IAQILSVPLSEHEQ---YFRNRLADIDTPTA-PF 226
Cdd:PRK12467  179 VTLHHIISDGWSMRVLVEELVQLYsaysQGREPSLPAlPIQYADYaIWQRSWLEAGERERqlaYWQEQLGGEHTVLElPT 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  227 DLVDVQGNGEDITEARLSLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLgaDRVMG 306
Cdd:PRK12467  259 DRPRPAVPSYRGARLRVDLPQALSAGLKALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANRNRVET--ERLIG 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  307 MFINTLPLRVSLRER-SVHDVVQATSHELMMLLAHEQAP---LALAQQCSQVPPPLPLFSTLFNYRHSQKDASSQFWEGM 382
Cdd:PRK12467  337 FFVNTQVLKAEVDPQaSFLELLQQVKRTALGAQAHQDLPfeqLVEALQPERSLSHSPLFQVMFNHQNTATGGRDREGAQL 416

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  383 RQLS------GRERTNYPITLSVDDLGDGFNLTAKTVMGVDPERIVHYMLTAIENLVTSLEKTPQQPALSQPILPKSERQ 456
Cdd:PRK12467  417 PGLTveelswARHTAQFDLALDTYESAQGLWAAFTYATDLFEATTIERLATHWRNLLEAIVAEPRRRLGELPLLDAEERA 496

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  457 QVLVDFNATDADFPREMlIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEM 536
Cdd:PRK12467  497 RELVRWNAPATEYAPDC-VHQLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEM 575

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  537 MVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLN--STLPTVLLDTPAA--AACPDTNPVVQgLH 612
Cdd:PRK12467  576 VVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQSHLLAQLPvpAGLRSLCLDEPADllCGYSGHNPEVA-LD 654

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  613 AAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRA 691
Cdd:PRK12467  655 PDNLAYVIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTElFGALASGATLHLLPPDCAR 734

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  692 DAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLgSDPAYQPAQVLIGGEAISPAVWSRLQSLSD-TRFINVYGPTECTVDA 770
Cdd:PRK12467  735 DAEAFAALMADQGVTVLKIVPSHLQALLQASR-VALPRPQRALVCGGEALQVDLLARVRALGPgARLINHYGPTETTVGV 813

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  771 TACVV---DRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSADpAARIY 847
Cdd:PRK12467  814 STYELsdeERDFGNVPIGQPLANLGLYILDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFGAD-GGRLY 892

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  848 KTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIArEDSPGDTRLVAYL--CARPDAELHPA 925
Cdd:PRK12467  893 RTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLA-QPGDAGLQLVAYLvpAAVADGAEHQA 971

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  926 ---ALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAfATRDYEAPQGGIETALAALWQELLGLDRVGR 1002
Cdd:PRK12467  972 trdELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPKPDASA-VQATFVAPQTELEKRLAAIWADVLKVERVGL 1050

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1003 HDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAVTATAHDAPFTLPVADRTQPLPLSFSQQRLWFLA 1081
Cdd:PRK12467 1051 TDNFFELGGHSLLATQVISRVRQRlGIQVPLRTLFEHQTLAGFAQAVAAQQQGAQPALPDVDRDQPLPLSYAQERQWFLW 1130

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1082 QLDPaASQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPD-TLGFSLSSHDLRKLDEAART 1160
Cdd:PRK12467 1131 QLEP-GSAAYHIPQALRLKGPLDIEALERSFDALVARHESLRTTFVQEDGRTRQVIHPVgSLTLEEPLLLAADKDEAQLK 1209

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1161 TRVaelaEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQY 1240
Cdd:PRK12467 1210 VYV----EAEARQPFDLEQGPLLRVGLLRLAADEHVLVLTLHHIVSDGWSMQVLVDELVALYAAYSQGQSLQLPALPIQY 1285

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1241 ADYAVWQRQWLQ-GETLNDLRdYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHALNRQQGTTLFMT 1319
Cdd:PRK12467 1286 ADYAVWQRQWMDaGERARQLA-YWKAQLGGEQPVLELPTDRPRPAVQSHRGARLAFELPPALAEGLRALARREGVTLFML 1364

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1320 LLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALDAYAHQALPFEQV 1399
Cdd:PRK12467 1365 LLASFQTLLHRYSGQDDIRVGVPIANRNRAETEGLIGFFVNTQVLRAEVDGQASFQQLLQQVKQAALEAQAHQDLPFEQL 1444

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1400 VEILQPARSLSYSPIFQVMLSLNNTPAQAL-TLPDLTLSAVERPQHSTHFDLSLSLIETENGLNGGLVYATDLFDRETIL 1478
Cdd:PRK12467 1445 VEALQPERSLSHSPLFQVMFNHQRDDHQAQaQLPGLSVESLSWESQTAQFDLTLDTYESSEGLQASLTYATDLFEASTIE 1524

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1479 RVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDA 1558
Cdd:PRK12467 1525 RLAGHWLNLLQGLVADPERRLGELDLLDEAERRQILEGWNATHTGYPLARLVHQLIEDQAAATPEAVALVFGEQELTYGE 1604

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1559 LNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQAN--QR 1636
Cdd:PRK12467 1605 LNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSHlqAR 1684

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1637 ALLTGDVPRILLDTAD--FSHLSEDNPHVpGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVL 1714
Cdd:PRK12467 1685 LPLPDGLRSLVLDQEDdwLEGYSDSNPAV-NLAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVL 1763

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1715 QKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSG 1794
Cdd:PRK12467 1764 QFTSFAFDVSVWELFWPLINGARLVIAPPGAHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVEHPLSLRRVVCGG 1843

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1795 EALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDD--HRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHI 1871
Cdd:PRK12467 1844 EALEVEALRPWLERLpDTGLFNLYGPTETAVDVTHWTCRRKDleGRDSVPIGQPIANLSTYILDASLNPVPIGVAGELYL 1923

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1872 GGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGV 1951
Cdd:PRK12467 1924 GGVGLARGYLNRPALTAERFVADPF-GTVGSRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGV 2002

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1952 QEAVVVArEDSPGDTRLVAYLCPQ----PGVTPDPADLRQQLGQHLA----EYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK12467 2003 REAVVIA-QDGANGKQLVAYVVPTdpglVDDDEAQVALRAILKNHLKaslpEYMVPAHLVFLARMPLTPNGKLDRKALPA 2081

                        2090      2100      2110      2120      2130      2140      2150      2160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2024 PDQSAVaTRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGLALDVRGVFSTPVLSDMA 2103
Cdd:PRK12467 2082 PDASEL-QQAYVAPQSELEQRLAAIWQDVLGLEQVGLHDNFFELGGDSIISIQVVSRARQAGIRFTPKDLFQHQTVQSLA 2160

                        2170      2180      2190      2200      2210      2220      2230      2240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2104 qAILAHQDKPAVvvppnripADSTAITPDLlplvtltqpeidritdtvsggasniqdiyPLAPLQEgiLF--------HH 2175
Cdd:PRK12467 2161 -AVAQEGDGTVS--------IDQGPVTGDL-----------------------------PLLPIQQ--MFfaddiperHH 2200

                        2250      2260      2270      2280      2290      2300      2310      2320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2176 LLQEqgdtyllrsmVAFTHRERLDAFL--SALQQVIDRHDILRTAVCWQDLSqpvqvvWRQAIL-PINHFEP---TSPED 2249
Cdd:PRK12467 2201 WNQS----------VLLEPREALDAELleAALQALLVHHDALRLGFVQEDGG------WSAMHRaPEQERRPllwQVVVA 2264

                        2330      2340      2350      2360      2370      2380      2390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 2250 VLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLaLSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLP 2325
Cdd:PRK12467 2265 DKEELEALCEQAQRSLDLEEGPLLRAVLATLPDGSQRLL-LVIHHLVVDGVSWRILLEDLQTAYRQLQGGQPVKLP 2339
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 1051-2406 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 1218.55  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1051 TAHDAPFTLPVADRTQPLPLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSID 1130
Cdd:COG1020     1 AAAAAAAALPPAAAAAPLPLSAAQQRLWLLLLLLLG-SAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRTRA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1131 GQPAQQIDPDtLGFSLSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWS 1210
Cdd:COG1020    80 GRPVQVIQPV-VAAPLPVVVLLVDLEALAEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLLLLLLLLLALHHIISDGLS 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1211 IGILARELAALYQAALEGSEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAG 1290
Cdd:COG1020   159 DGLLLAELLRLYLAAYAGAPLPLPPLPIQYADYALWQREWLQGEELARQLAYWRQQLAGLPPLLELPTDRPRPAVQSYRG 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1291 DQVPFHLDAGQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGR 1370
Cdd:COG1020   239 ARVSFRLPAELTAALRALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGRPRPELEGLVGFFVNTLPLRVDLSG 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1371 CHAVADLLDQVRERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQALTLPDLTLSAVERPQHSTHFDL 1450
Cdd:COG1020   319 DPSFAELLARVRETLLAAYAHQDLPFERLVEELQPERDLSRNPLFQVMFVLQNAPADELELPGLTLEPLELDSGTAKFDL 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1451 SLSLIETENGLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDALI 1530
Cdd:COG1020   399 TLTVVETGDGLRLTLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQLLAEWNATAAPYPADATL 478

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDP 1610
Cdd:COG1020   479 HELFEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDP 558

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1611 GYPAERLAYMLDDASPVALLTQANQRALLTG-DVPRILLDTADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMN 1689
Cdd:COG1020   559 AYPAERLAYMLEDAGARLVLTQSALAARLPElGVPVLALDALALAAEPATNPPVP-VTPDDLAYVIYTSGSTGRPKGVMV 637

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPS 1769
Cdd:COG1020   638 EHRALVNLLAWMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLTPS 717

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1770 MLQQFVQWADAdcACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDDHRS-FVPIGRPIA 1847
Cdd:COG1020   718 LLRALLDAAPE--ALPSLRLVLVGGEALPPELVRRWRARLpGARLVNLYGPTETTVDSTYYEVTPPDADGgSVPIGRPIA 795

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1848 NTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDF 1927
Cdd:COG1020   796 NTRVYVLDAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPF-GFPGARLYRTGDLARWLPDGNLEFLGRADD 874

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1928 QVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDA 2007
Cdd:COG1020   875 QVKIRGFRIELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLP 954

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2008 FPLTPNGKLDRKALPAPDQSAVATrdYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGLA 2087
Cdd:COG1020   955 LPLTGNGKLDRLALPAPAAAAAAA--AAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAARLLLL 1032

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2088 LDVRGVFSTPVLSDMAQAILAHQDKPAVVVPPNRIPADSTAITPDLLPLVTLTQpeidritdtvsggasniqdiypLAPL 2167
Cdd:COG1020  1033 LLLLLLLFLAAAAAAAAAAAAAAAAAAAAPLAAAAAPLPLPPLLLSLLALLLAL----------------------LLLL 1090

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2168 QEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILPINHFEPTSP 2247
Cdd:COG1020  1091 ALLALLALLLLLLLLLLLLALLLLLALLLALLAALRARRAVRQEGPRLRLLVALAAALALAALLALLLAAAAAAAELLAA 1170

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2248 EDVLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLPYR 2327
Cdd:COG1020  1171 AALLLLLALLLLALLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLAAAAAALLALALLLALLALAALLALA 1250

                        1290      1300      1310      1320      1330      1340      1350
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 2328 NFIAQTLSVPNSAHEAYFRDKLADVDEPTAPFGLLNVQGSGGDIHEARLVLDATLASAIRQQARHLGVSPGVLFHVAWA 2406
Cdd:COG1020  1251 ALAALAAALLALALALLALALLLLALALLLPALARARAARTARALALLLLLALLLLLALALALLLLLLLLLALLLLALL 1329
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 2159-3191 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 868.40  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2159 QDIYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVC--WQDLSQPVQVVWRQAI 2236
Cdd:COG1020    15 AAPLPLSAAQQRLWLLLLLLLGSAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRtrAGRPVQVIQPVVAAPL 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2237 LPINHFEPTSPEDVLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQnEWLLALSFHHLISDHMTLALIVGEIRLLLQHQ 2316
Cdd:COG1020    95 PVVVLLVDLEALAEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLLL-LLLLLLALHHIISDGLSDGLLLAELLRLYLAA 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2317 ADALPTPLP---------YRNFIAQTLSVPNSAHEAYFRDKLA-DVDEPTAPFGLLNVQGSGGDIHEARLVLDATLASAI 2386
Cdd:COG1020   174 YAGAPLPLPplpiqyadyALWQREWLQGEELARQLAYWRQQLAgLPPLLELPTDRPRPAVQSYRGARVSFRLPAELTAAL 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2387 RQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISL-ADRGAAEVVERTSH 2465
Cdd:COG1020   254 RALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGR--PRPELEGLVGFFVNTLPLRVDLsGDPSFAELLARVRE 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2466 DLMTLLEHEQAPLALAQR---CSGVAPPMPLFSTLLNYRHTQASSTDNTLSDIRVLT-SEERTNYPLTLAVDDRGEGFSL 2541
Cdd:COG1020   332 TLLAAYAHQDLPFERLVEelqPERDLSRNPLFQVMFVLQNAPADELELPGLTLEPLElDSGTAKFDLTLTVVETGDGLRL 411

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2542 VAQTLEDIDPHRLLNYLMTAISSLVDALETEPQRSILNLPVLPDSERQQMLVDFNATDADIPRHALIHELFEAQVACTPD 2621
Cdd:COG1020   412 TLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQLLAEWNATAAPYPADATLHELFEAQAARTPD 491

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2622 AIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDD 2701
Cdd:COG1020   492 AVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAERLAYMLED 571

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2702 AKPVALISQSAHLG-IMNGSLPVILLDDGEtrpFDNEPDTPLDArkqGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNF 2780
Cdd:COG1020   572 AGARLVLTQSALAArLPELGVPVLALDALA---LAAEPATNPPV---PVTPDDLAYVIYTSGSTGRPKGVMVEHRALVNL 645

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2781 LCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVEL 2860
Cdd:COG1020   646 LAWMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLTPSLLRALLDA 725

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2861 RDFALPPGFKALCGGEALPENLATALLQKV--TTLWNLYGPTETTIWSTLNGLTTPTPY-----IGHPIANTQIYILDAQ 2933
Cdd:COG1020   726 APEALPSLRLVLVGGEALPPELVRRWRARLpgARLVNLYGPTETTVDSTYYEVTPPDADggsvpIGRPIANTRVYVLDAH 805

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2934 GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIE 3013
Cdd:COG1020   806 LQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPF-GFPGARLYRTGDLARWLPDGNLEFLGRADDQVKIRGFRIE 884

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3014 LGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLD 3093
Cdd:COG1020   885 LGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLD 964

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3094 RKALPAPDQSAMATrgYEAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQH 3173
Cdd:COG1020   965 RLALPAPAAAAAAA--AAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAARLLLLLLLLLLLFLA 1042

                        1050
                  ....*....|....*...
gi 641744967 3174 PQLSALAEVILAAQIHAA 3191
Cdd:COG1020  1043 AAAAAAAAAAAAAAAAAA 1060
PRK12316 PRK12316
peptide synthase; Provisional
 2129-3186 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 803.41  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2129 ITPDLLPLVTLTQPEIDritdTVSGGASNIQDIYPLAPLQEGILFHHLLQEQGDTYL--LRSMVAFTHRERldaFLSALQ 2206
Cdd:PRK12316 4074 VTPSDFPLAGLDQARLD----ALPLPLGEIEDIYPLSPMQQGMLFHSLYEQEAGDYInqMRVDVQGLDVER---FRAAWQ 4146

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2207 QVIDRHDILRTAVCWQD-LSQPVQVVWRQAILPINHFEPTSPEDVLAQLQA-HTEPRTRRIDLSQAPLFRADIAHDPlQN 2284
Cdd:PRK12316 4147 AALDRHDVLRSGFVWQGeLGRPLQVVHKQVSLPFAELDWRGRADLQAALDAlAAAERERGFDLQRAPLLRLVLVRTA-EG 4225

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2285 EWLLALSFHHLISDHMTLALIVGEIrllLQHQADALPTP--LPYRNFIAQTLSVPNSAHEAYFRDKLADVDEPT------ 2356
Cdd:PRK12316 4226 RHHLIYTNHHILMDGWSNSQLLGEV---LERYSGRPPAQpgGRYRDYIAWLQRQDAAASEAFWREQLAALDEPTrlaqai 4302

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2357 APFGLLNVQGSGGDIHEarlvLDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADR 2436
Cdd:PRK12316 4303 ARADLRSANGYGEHVRE----LDATATARLREFARTQRVTLNTLVQAAWLLLLQRYTGQDTVAFGATVAGRPAELPGIEG 4378

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2437 IMGMFINTLPLRISL-ADRGAAEVVERTSHDLMTLLEHEQAPLALAQRCSGVAPPmPLFSTLLNYRHTQASST--DNTLS 2513
Cdd:PRK12316 4379 QIGLFINTLPVIATPrAQQSVVEWLQQVQRQNLALREHEHTPLYEIQRWAGQGGE-ALFDSLLVFENYPVSEAlqQGAPG 4457

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2514 DIRV--LTSEERTNYPLTLAVDdRGEGFSL---------VAQTLEDIDPHrllnylmtaISSLVDALETEPQRSILNLPV 2582
Cdd:PRK12316 4458 GLRFgeVTNHEQTNYPLTLAVG-LGETLSLqfsydrghfDAATIERLARH---------LTNLLEAMAEDPQRRLGELQL 4527

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2583 LPDSERQQMLVDFNATDADIPRHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAIC 2662
Cdd:PRK12316 4528 LEKAEQQRIVALWNRTDAGYPATRCVHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIA 4607

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2663 VERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQS---AHLGIMNG--SLPVILLDDGETRPfDNE 2737
Cdd:PRK12316 4608 MERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTQShllQRLPIPDGlaSLALDRDEDWEGFP-AHD 4686

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2738 PDTPLDarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLN 2817
Cdd:PRK12316 4687 PAVRLH-------PDNLAYVIYTSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSFDGSHEGLYHPLIN 4759

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2818 GARLILATQAQAADAQQLAmLIERHAVSFMQATPSTWRMLVE--LRDFALPPGFKALCGGEALPENLATALLQ--KVTTL 2893
Cdd:PRK12316 4760 GASVVIRDDSLWDPERLYA-EIHEHRVTVLVFPPVYLQQLAEhaERDGEPPSLRVYCFGGEAVAQASYDLAWRalKPVYL 4838

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2894 WNLYGPTETTIWSTLNGLTTPTP------YIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERF 2967
Cdd:PRK12316 4839 FNGYGPTETTVTVLLWKARDGDAcgaaymPIGTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVARGYLERPALTAERF 4918

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2968 ITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGdKRLVAY 3047
Cdd:PRK12316 4919 VPDPF-GAPGGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAVG-KQLVGY 4996

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3048 LLAQpDTVLEPAD---------LRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPDQSAmATRGYEAPQGDLE 3118
Cdd:PRK12316 4997 VVPQ-DPALADADeaqaelrdeLKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKALPQPDASL-LQQAYVAPRSELE 5074

                        1050      1060      1070      1080      1090      1100
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 3119 HALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILAA 3186
Cdd:PRK12316 5075 QQVAAIWAEVLQLERVGLDDNFFELGGHSLLAIQVTSRIQLELGLELPLRELFQTPTLAAFVELAAAA 5142
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
 1531-2021 0e+00

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 745.64  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDP 1610
Cdd:cd17646     1 HALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1611 GYPAERLAYMLDDASPVALLTQANQRALLTGDVPRILLDTADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMNS 1690
Cdd:cd17646    81 GYPADRLAYMLADAGPAVVLTTADLAARLPAGGDVALLGDEALAAPPATPPLVP-PRPDNLAYVIYTSGSTGRPKGVMVT 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1691 HRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSM 1770
Cdd:cd17646   160 HAGIVNRLLWMQDEYPLGPGDRVLQKTPLSFDVSVWELFWPLVAGARLVVARPGGHRDPAYLAALIREHGVTTCHFVPSM 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1771 LQQFVQWADADcACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHRSFVPIGRPIANTQ 1850
Cdd:cd17646   240 LRVFLAEPAAG-SCASLRRVFCSGEALPPELAARFLALPGAELHNLYGPTEAAIDVTHWPVRGPAETPSVPIGRPVPNTR 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1851 LYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQVK 1930
Cdd:cd17646   319 LYVLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAERFVPDPF--GPGSRMYRTGDLARWRPDGALEFLGRSDDQVK 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1931 LRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVT-PDPADLRQQLGQHLAEYMVPGAFVTLDAFP 2009
Cdd:cd17646   397 IRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGAAgPDTAALRAHLAERLPEYMVPAAFVVLDALP 476

                         490
                  ....*....|..
gi 641744967 2010 LTPNGKLDRKAL 2021
Cdd:cd17646   477 LTANGKLDRAAL 488
E-C_NRPS cd19544
Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); ...
 2161-2569 0e+00

Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); Dual function Epimerization/Condensation (E/C) domains have both an epimerization and a DCL condensation activity. Dual E/C domains first epimerize the substrate amino acid to produce a D-configuration, then catalyze the condensation between the D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. They are D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. These Dual E/C domains contain an extended His-motif (HHx(N)GD) near the N-terminus of the domain in addition to the standard Condensation (C) domain active site motif (HHxxxD). C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains, these include the DCL-type, LCL-type, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C domains, and the X-domain.


Pssm-ID: 380466 [Multi-domain]  Cd Length: 413  Bit Score: 701.50  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2161 IYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILPIN 2240
Cdd:cd19544     1 IYPLAPLQEGILFHHLLAEEGDPYLLRSLLAFDSRARLDAFLAALQQVIDRHDILRTAILWEGLSEPVQVVWRQAELPVE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2241 HFEPTSPEDVLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADAL 2320
Cdd:cd19544    81 ELTLDPGDDALAQLRARFDPRRYRLDLRQAPLLRAHVAEDPANGRWLLLLLFHHLISDHTSLELLLEEIQAILAGRAAAL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2321 PTPLPYRNFIAQTLSVPNSA-HEAYFRDKLADVDEPTAPFGLLNVQGSGGDIHEARLVLDATLASAIRQQARHLGVSPGV 2399
Cdd:cd19544   161 PPPVPYRNFVAQARLGASQAeHEAFFREMLGDVDEPTAPFGLLDVQGDGSDITEARLALDAELAQRLRAQARRLGVSPAS 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2400 LFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADRIMGMFINTLPLRISLADRGAAEVVERTSHDLMTLLEHEQAPLA 2479
Cdd:cd19544   241 LFHLAWALVLARCSGRDDVVFGTVLSGRMQGGAGADRALGMFINTLPLRVRLGGRSVREAVRQTHARLAELLRHEHASLA 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2480 LAQRCSGVAPPMPLFSTLLNYRHTQASSTDN---TLSDIRVLTSEERTNYPLTLAVDDRGEGFSLVAQTLEDIDPHRLLN 2556
Cdd:cd19544   321 LAQRCSGVPAPTPLFSALLNYRHSAAAAAAAalaAWEGIELLGGEERTNYPLTLSVDDLGDGFSLTAQVVAPIDAERVCA 400

                         410
                  ....*....|...
gi 641744967 2557 YLMTAISSLVDAL 2569
Cdd:cd19544   401 YMETALEQLVDAL 413
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
 1555-1956 2.69e-174

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 542.63  E-value: 2.69e-174
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1555 TYDALNRRANQLAHHLIDL-GVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA 1633
Cdd:TIGR01733    1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1634 NQRALLTGDVPRILLDTADFSHLSEDNPHVPGLDAH----HLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTP 1709
Cdd:TIGR01733   81 ALASRLAGLVLPVILLDPLELAALDDAPAPPPPDAPsgpdDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGLDP 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1710 DDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKD-AAYLAQLIERTGITTLHFVPSMLQQFVQWADAdcACDSLR 1788
Cdd:TIGR01733  161 DDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDEERDdAALLAALIAEHPVTVLNLTPSLLALLAAALPP--ALASLR 238

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1789 RVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDDHRSF--VPIGRPIANTQLYILDTLGQPVPLGV 1865
Cdd:TIGR01733  239 LVILGGEALTPALVDRWRARGpGARLINLYGPTETTVWSTATLVDPDDAPREspVPIGRPLANTRLYVLDDDLRPVPVGV 318

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1866 AGELHIGGVGVARGYLNRPDLTAERFIPDPFINQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARL 1945
Cdd:TIGR01733  319 VGELYIGGPGVARGYLNRPELTAERFVPDPFAGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAAL 398

                          410
                   ....*....|.
gi 641744967  1946 MQCPGVQEAVV 1956
Cdd:TIGR01733  399 LRHPGVREAVV 409
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
 2633-3032 1.22e-165

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 517.59  E-value: 1.22e-165
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2633 SYDELNRRANRLAHHLIS-FGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQS 2711
Cdd:TIGR01733    1 TYRELDERANRLARHLRAaGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2712 AHLGIMNG-SLPVILLDDGETRPFDNEPDTPLDARKQGltPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGI 2790
Cdd:TIGR01733   81 ALASRLAGlVLPVILLDPLELAALDDAPAPPPPDAPSG--PDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGL 158

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2791 AQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAM-LIERHAVSFMQATPSTWRMLVELRDFALPpGF 2869
Cdd:TIGR01733  159 DPDDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDEERDDAALLAaLIAEHPVTVLNLTPSLLALLAAALPPALA-SL 237

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2870 KALC-GGEALPENLATALLQKV--TTLWNLYGPTETTIWST-------LNGLTTPTPyIGHPIANTQIYILDAQGRVVPL 2939
Cdd:TIGR01733  238 RLVIlGGEALTPALVDRWRARGpgARLINLYGPTETTVWSTatlvdpdDAPRESPVP-IGRPLANTRLYVLDDDLRPVPV 316

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2940 GVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIET 3019
Cdd:TIGR01733  317 GVVGELYIGGPGVARGYLNRPELTAERFVPDPFAGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEA 396

                          410
                   ....*....|...
gi 641744967  3020 RLARCHGVHDAVV 3032
Cdd:TIGR01733  397 ALLRHPGVREAVV 409
AMP-binding pfam00501
AMP-binding enzyme;
1534-1932 8.64e-128

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 409.39  E-value: 8.64e-128
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1534 VEDQAARTPDTTAVL-FEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGY 1612
Cdd:pfam00501    1 LERQAARTPDKTALEvGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1613 PAERLAYMLDDASPVALLTQANQRA------------------LLTGDVPRILLDTADFSHLSEDNPHVPGLDAHHLAYV 1674
Cdd:pfam00501   81 PAEELAYILEDSGAKVLITDDALKLeellealgklevvklvlvLDRDPVLKEEPLPEEAKPADVPPPPPPPPDPDDLAYI 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1675 IYTSGSTGKPKGVMNSHRALCNRLVWMQNTY----RLTPDDRVLQKTPFSFDVSV-WEFFWPLLYGARLVMARPDGHKDA 1749
Cdd:pfam00501  161 IYTSGTTGKPKGVMLTHRNLVANVLSIKRVRprgfGLGPDDRVLSTLPLFHDFGLsLGLLGPLLAGATVVLPPGFPALDP 240

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1750 AYLAQLIERTGITTLHFVPSMLQQFVQ-WADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTF 1828
Cdd:pfam00501  241 AALLELIERYKVTVLYGVPTLLNMLLEaGAPKRALLSSLRLVLSGGAPLPPELARRFRELFGGALVNGYGLTETTGVVTT 320

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1829 WACQPDDHRSFVPIGRPIANTQLYILDTL-GQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDpfinqpgaRLYKT 1907
Cdd:pfam00501  321 PLPLDEDLRSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDED--------GWYRT 392

                          410       420
                   ....*....|....*....|....*
gi 641744967  1908 GDLARWLPDGSLEYLGRNDFQVKLR 1932
Cdd:pfam00501  393 GDLGRRDEDGYLEIVGRKKDQIKLG 417
AMP-binding pfam00501
AMP-binding enzyme;
2612-3008 2.46e-116

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 376.27  E-value: 2.46e-116
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2612 FEAQVACTPDAIAVVFGE-ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTY 2690
Cdd:pfam00501    1 LERQAARTPDKTALEVGEgRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2691 PVERLRYMLDDAKPVALISQSAHLGIMNGSL--------PVILLD-----DGETRPFDNEPDTPLDARKQGLTPRHLAYV 2757
Cdd:pfam00501   81 PAEELAYILEDSGAKVLITDDALKLEELLEAlgklevvkLVLVLDrdpvlKEEPLPEEAKPADVPPPPPPPPDPDDLAYI 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2758 IYTSGSTGKPKGVMVEHANMVNFLCSMRKEP----GIAQEDVLLGVTSLSFDIS-ILEIFLPLLNGARLILATQAQAADA 2832
Cdd:pfam00501  161 IYTSGTTGKPKGVMLTHRNLVANVLSIKRVRprgfGLGPDDRVLSTLPLFHDFGlSLGLLGPLLAGATVVLPPGFPALDP 240

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2833 QQLAMLIERHAVSFMQATPSTWRMLVELRDF--ALPPGFKA-LCGGEALPENLATALLQKV-TTLWNLYGPTETTIWST- 2907
Cdd:pfam00501  241 AALLELIERYKVTVLYGVPTLLNMLLEAGAPkrALLSSLRLvLSGGAPLPPELARRFRELFgGALVNGYGLTETTGVVTt 320

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2908 ---LNGLTTPTPYIGHPIANTQIYILDAQ-GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDpfsgapeaRMYKT 2983
Cdd:pfam00501  321 plpLDEDLRSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDED--------GWYRT 392

                          410       420
                   ....*....|....*....|....*
gi 641744967  2984 GDLGRWLPDGTLEYLGRNDFQVKVR 3008
Cdd:pfam00501  393 GDLGRRDEDGYLEIVGRKKDQIKLG 417
PKS_PP smart00823
Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the ...
 3111-3185 5.12e-06

Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the prosthetic group of acyl carrier proteins (ACP) in some multienzyme complexes where it serves as a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups.


Pssm-ID: 214834 [Multi-domain]  Cd Length: 86  Bit Score: 46.86  E-value: 5.12e-06
                            10        20        30        40        50        60        70
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967   3111 EAPQGDLEHALAQIWQTLLGV---ERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILA 3185
Cdd:smart00823    7 AERRRLLLDLVREQVAAVLGHaaaEAIDPDRPFRDLGLDSLMAVELRNRLEAATGLRLPATLVFDHPTPAALAEHLAA 84
PKS_PP smart00823
Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the ...
 972-1050 7.47e-05

Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the prosthetic group of acyl carrier proteins (ACP) in some multienzyme complexes where it serves as a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups.


Pssm-ID: 214834 [Multi-domain]  Cd Length: 86  Bit Score: 43.78  E-value: 7.47e-05
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    972 ATRDYEAPQGGIETALAALWQELLGL---DRVGRHDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAA 1047
Cdd:smart00823    2 AALPPAERRRLLLDLVREQVAAVLGHaaaEAIDPDRPFRDLGLDSLMAVELRNRLEAAtGLRLPATLVFDHPTPAALAEH 81


                    ...
gi 641744967   1048 VTA 1050
Cdd:smart00823   82 LAA 84
 
Name Accession Description Interval E-value
PRK12467 PRK12467
peptide synthase; Provisional
 3-2325 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 1873.31  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    3 LVTLSQEEIDTVIATVPDGVANVQDIyPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDIL 82
Cdd:PRK12467   25 LEKMQEEGVSFANLPIPQVRSAFERI-PLSYAQERQWFLWQLDPDSAAYNIPTALRLRGELDVSALRRAFDALVARHESL 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   83 RTAICWQGlHQPVQVVWRQAPLTV--NTLTTTSSDTVPAQLRAATDPSNHRL-NLSNAPLLSAT---TAHDpvcgEWLLS 156
Cdd:PRK12467  104 RTRFVQDE-EGFRQVIDASLSLTIplDDLANEQGRARESQIEAYINEEVARPfDLANGPLLRVRllrLADD----EHVLV 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  157 LSIHHLISDHITQALIIDEIRLLL----EDRPEALPK-PLPYRNF-IAQILSVPLSEHEQ---YFRNRLADIDTPTA-PF 226
Cdd:PRK12467  179 VTLHHIISDGWSMRVLVEELVQLYsaysQGREPSLPAlPIQYADYaIWQRSWLEAGERERqlaYWQEQLGGEHTVLElPT 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  227 DLVDVQGNGEDITEARLSLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLgaDRVMG 306
Cdd:PRK12467  259 DRPRPAVPSYRGARLRVDLPQALSAGLKALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANRNRVET--ERLIG 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  307 MFINTLPLRVSLRER-SVHDVVQATSHELMMLLAHEQAP---LALAQQCSQVPPPLPLFSTLFNYRHSQKDASSQFWEGM 382
Cdd:PRK12467  337 FFVNTQVLKAEVDPQaSFLELLQQVKRTALGAQAHQDLPfeqLVEALQPERSLSHSPLFQVMFNHQNTATGGRDREGAQL 416

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  383 RQLS------GRERTNYPITLSVDDLGDGFNLTAKTVMGVDPERIVHYMLTAIENLVTSLEKTPQQPALSQPILPKSERQ 456
Cdd:PRK12467  417 PGLTveelswARHTAQFDLALDTYESAQGLWAAFTYATDLFEATTIERLATHWRNLLEAIVAEPRRRLGELPLLDAEERA 496

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  457 QVLVDFNATDADFPREMlIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEM 536
Cdd:PRK12467  497 RELVRWNAPATEYAPDC-VHQLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEM 575

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  537 MVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLN--STLPTVLLDTPAA--AACPDTNPVVQgLH 612
Cdd:PRK12467  576 VVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQSHLLAQLPvpAGLRSLCLDEPADllCGYSGHNPEVA-LD 654

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  613 AAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRA 691
Cdd:PRK12467  655 PDNLAYVIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTElFGALASGATLHLLPPDCAR 734

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  692 DAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLgSDPAYQPAQVLIGGEAISPAVWSRLQSLSD-TRFINVYGPTECTVDA 770
Cdd:PRK12467  735 DAEAFAALMADQGVTVLKIVPSHLQALLQASR-VALPRPQRALVCGGEALQVDLLARVRALGPgARLINHYGPTETTVGV 813

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  771 TACVV---DRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSADpAARIY 847
Cdd:PRK12467  814 STYELsdeERDFGNVPIGQPLANLGLYILDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFGAD-GGRLY 892

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  848 KTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIArEDSPGDTRLVAYL--CARPDAELHPA 925
Cdd:PRK12467  893 RTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLA-QPGDAGLQLVAYLvpAAVADGAEHQA 971

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  926 ---ALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAfATRDYEAPQGGIETALAALWQELLGLDRVGR 1002
Cdd:PRK12467  972 trdELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPKPDASA-VQATFVAPQTELEKRLAAIWADVLKVERVGL 1050

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1003 HDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAVTATAHDAPFTLPVADRTQPLPLSFSQQRLWFLA 1081
Cdd:PRK12467 1051 TDNFFELGGHSLLATQVISRVRQRlGIQVPLRTLFEHQTLAGFAQAVAAQQQGAQPALPDVDRDQPLPLSYAQERQWFLW 1130

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1082 QLDPaASQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPD-TLGFSLSSHDLRKLDEAART 1160
Cdd:PRK12467 1131 QLEP-GSAAYHIPQALRLKGPLDIEALERSFDALVARHESLRTTFVQEDGRTRQVIHPVgSLTLEEPLLLAADKDEAQLK 1209

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1161 TRVaelaEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQY 1240
Cdd:PRK12467 1210 VYV----EAEARQPFDLEQGPLLRVGLLRLAADEHVLVLTLHHIVSDGWSMQVLVDELVALYAAYSQGQSLQLPALPIQY 1285

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1241 ADYAVWQRQWLQ-GETLNDLRdYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHALNRQQGTTLFMT 1319
Cdd:PRK12467 1286 ADYAVWQRQWMDaGERARQLA-YWKAQLGGEQPVLELPTDRPRPAVQSHRGARLAFELPPALAEGLRALARREGVTLFML 1364

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1320 LLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALDAYAHQALPFEQV 1399
Cdd:PRK12467 1365 LLASFQTLLHRYSGQDDIRVGVPIANRNRAETEGLIGFFVNTQVLRAEVDGQASFQQLLQQVKQAALEAQAHQDLPFEQL 1444

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1400 VEILQPARSLSYSPIFQVMLSLNNTPAQAL-TLPDLTLSAVERPQHSTHFDLSLSLIETENGLNGGLVYATDLFDRETIL 1478
Cdd:PRK12467 1445 VEALQPERSLSHSPLFQVMFNHQRDDHQAQaQLPGLSVESLSWESQTAQFDLTLDTYESSEGLQASLTYATDLFEASTIE 1524

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1479 RVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDA 1558
Cdd:PRK12467 1525 RLAGHWLNLLQGLVADPERRLGELDLLDEAERRQILEGWNATHTGYPLARLVHQLIEDQAAATPEAVALVFGEQELTYGE 1604

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1559 LNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQAN--QR 1636
Cdd:PRK12467 1605 LNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSHlqAR 1684

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1637 ALLTGDVPRILLDTAD--FSHLSEDNPHVpGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVL 1714
Cdd:PRK12467 1685 LPLPDGLRSLVLDQEDdwLEGYSDSNPAV-NLAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVL 1763

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1715 QKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSG 1794
Cdd:PRK12467 1764 QFTSFAFDVSVWELFWPLINGARLVIAPPGAHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVEHPLSLRRVVCGG 1843

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1795 EALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDD--HRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHI 1871
Cdd:PRK12467 1844 EALEVEALRPWLERLpDTGLFNLYGPTETAVDVTHWTCRRKDleGRDSVPIGQPIANLSTYILDASLNPVPIGVAGELYL 1923

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1872 GGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGV 1951
Cdd:PRK12467 1924 GGVGLARGYLNRPALTAERFVADPF-GTVGSRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGV 2002

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1952 QEAVVVArEDSPGDTRLVAYLCPQ----PGVTPDPADLRQQLGQHLA----EYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK12467 2003 REAVVIA-QDGANGKQLVAYVVPTdpglVDDDEAQVALRAILKNHLKaslpEYMVPAHLVFLARMPLTPNGKLDRKALPA 2081

                        2090      2100      2110      2120      2130      2140      2150      2160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2024 PDQSAVaTRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGLALDVRGVFSTPVLSDMA 2103
Cdd:PRK12467 2082 PDASEL-QQAYVAPQSELEQRLAAIWQDVLGLEQVGLHDNFFELGGDSIISIQVVSRARQAGIRFTPKDLFQHQTVQSLA 2160

                        2170      2180      2190      2200      2210      2220      2230      2240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2104 qAILAHQDKPAVvvppnripADSTAITPDLlplvtltqpeidritdtvsggasniqdiyPLAPLQEgiLF--------HH 2175
Cdd:PRK12467 2161 -AVAQEGDGTVS--------IDQGPVTGDL-----------------------------PLLPIQQ--MFfaddiperHH 2200

                        2250      2260      2270      2280      2290      2300      2310      2320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2176 LLQEqgdtyllrsmVAFTHRERLDAFL--SALQQVIDRHDILRTAVCWQDLSqpvqvvWRQAIL-PINHFEP---TSPED 2249
Cdd:PRK12467 2201 WNQS----------VLLEPREALDAELleAALQALLVHHDALRLGFVQEDGG------WSAMHRaPEQERRPllwQVVVA 2264

                        2330      2340      2350      2360      2370      2380      2390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 2250 VLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLaLSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLP 2325
Cdd:PRK12467 2265 DKEELEALCEQAQRSLDLEEGPLLRAVLATLPDGSQRLL-LVIHHLVVDGVSWRILLEDLQTAYRQLQGGQPVKLP 2339
PRK12467 PRK12467
peptide synthase; Provisional
 1061-3182 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 1756.21  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1061 VADRTQPLPLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPD 1140
Cdd:PRK12467   43 VRSAFERIPLSYAQERQWFLWQLDPD-SAAYNIPTALRLRGELDVSALRRAFDALVARHESLRTRFVQDEEGFRQVIDAS 121

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1141 tLGFSLSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAA 1220
Cdd:PRK12467  122 -LSLTIPLDDLANEQGRARESQIEAYINEEVARPFDLANGPLLRVRLLRLADDEHVLVVTLHHIISDGWSMRVLVEELVQ 200

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1221 LYQAALEGSEANLPPLPVQYADYAVWQRQWLQ-GETLNDLRdYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDA 1299
Cdd:PRK12467  201 LYSAYSQGREPSLPALPIQYADYAIWQRSWLEaGERERQLA-YWQEQLGGEHTVLELPTDRPRPAVPSYRGARLRVDLPQ 279

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1300 GQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLD 1379
Cdd:PRK12467  280 ALSAGLKALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANRNRVETERLIGFFVNTQVLKAEVDPQASFLELLQ 359

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1380 QVRERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPA-----QALTLPDLTLSAVERPQHSTHFDLSLSL 1454
Cdd:PRK12467  360 QVKRTALGAQAHQDLPFEQLVEALQPERSLSHSPLFQVMFNHQNTATggrdrEGAQLPGLTVEELSWARHTAQFDLALDT 439

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1455 IETENGLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDAlIHQLV 1534
Cdd:PRK12467  440 YESAQGLWAAFTYATDLFEATTIERLATHWRNLLEAIVAEPRRRLGELPLLDAEERARELVRWNAPATEYAPDC-VHQLI 518

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1535 EDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPA 1614
Cdd:PRK12467  519 EAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQ 598

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1615 ERLAYMLDDASPVALLTQANQRALLT--GDVPRILLDTAD--FSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMNS 1690
Cdd:PRK12467  599 DRLAYMLDDSGVRLLLTQSHLLAQLPvpAGLRSLCLDEPAdlLCGYSGHNPEVA-LDPDNLAYVIYTSGSTGQPKGVAIS 677

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1691 HRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSM 1770
Cdd:PRK12467  678 HGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGATLHLLPPDCARDAEAFAALMADQGVTVLKIVPSH 757

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1771 LQQFVQWADADcACDSLRRVICSGEALPAELQQRFFA-RFNAQLHNLYGPTEAAIDVTFWACQPDDHRS-FVPIGRPIAN 1848
Cdd:PRK12467  758 LQALLQASRVA-LPRPQRALVCGGEALQVDLLARVRAlGPGARLINHYGPTETTVGVSTYELSDEERDFgNVPIGQPLAN 836

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1849 TQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQ 1928
Cdd:PRK12467  837 LGLYILDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPF-GADGGRLYRTGDLARYRADGVIEYLGRMDHQ 915

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1929 VKLRGFRIELGEIEARLMQCPGVQEAVVVArEDSPGDTRLVAYLCpqPGVTPDPA-------DLRQQLGQHLAEYMVPGA 2001
Cdd:PRK12467  916 VKIRGFRIELGEIEARLLAQPGVREAVVLA-QPGDAGLQLVAYLV--PAAVADGAehqatrdELKAQLRQVLPDYMVPAH 992

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2002 FVTLDAFPLTPNGKLDRKALPAPDQSAVaTRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERL 2081
Cdd:PRK12467  993 LLLLDSLPLTPNGKLDRKALPKPDASAV-QATFVAPQTELEKRLAAIWADVLKVERVGLTDNFFELGGHSLLATQVISRV 1071

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2082 RRA-GLALDVRGVFSTPVLSDMAQAILAHQdkpavvvpPNRIPAdstaitpdlLPLVTLTQPEidritdtvsggasniqd 2160
Cdd:PRK12467 1072 RQRlGIQVPLRTLFEHQTLAGFAQAVAAQQ--------QGAQPA---------LPDVDRDQPL----------------- 1117

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2161 iyPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCwQDLSQPVQVVWRQAILPIN 2240
Cdd:PRK12467 1118 --PLSYAQERQWFLWQLEPGSAAYHIPQALRLKGPLDIEALERSFDALVARHESLRTTFV-QEDGRTRQVIHPVGSLTLE 1194

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2241 HFEPTSPEDVLAQLQAHTEPRTRR-IDLSQAPLFRAD---IAHDplqnEWLLALSFHHLISDHMTLALIVGEI----RLL 2312
Cdd:PRK12467 1195 EPLLLAADKDEAQLKVYVEAEARQpFDLEQGPLLRVGllrLAAD----EHVLVLTLHHIVSDGWSMQVLVDELvalyAAY 1270

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2313 LQHQADALPT-PLPYRNFIA---QTLSVPNSAHE-AYFRDKLA---DVDEPTAPFGLLNVQGSGGDIHeaRLVLDATLAS 2384
Cdd:PRK12467 1271 SQGQSLQLPAlPIQYADYAVwqrQWMDAGERARQlAYWKAQLGgeqPVLELPTDRPRPAVQSHRGARL--AFELPPALAE 1348

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2385 AIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISLadRGAAEVVERTS 2464
Cdd:PRK12467 1349 GLRALARREGVTLFMLLLASFQTLLHRYSGQDDIRVGVPIANR--NRAETEGLIGFFVNTQVLRAEV--DGQASFQQLLQ 1424

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2465 HDLMTLLE---HEQAPlaLAQRCSGVAPPM-----PLFSTLLNYRhTQASSTDNTLSDIRV--LTSEERT-NYPLTLAVD 2533
Cdd:PRK12467 1425 QVKQAALEaqaHQDLP--FEQLVEALQPERslshsPLFQVMFNHQ-RDDHQAQAQLPGLSVesLSWESQTaQFDLTLDTY 1501

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2534 DRGEGFSLVAQTLEDIDPHRLLNYLMTAISSLVDALETEPQRSILNLPVLPDSERQQMLVDFNATDADIPRHALIHELFE 2613
Cdd:PRK12467 1502 ESSEGLQASLTYATDLFEASTIERLAGHWLNLLQGLVADPERRLGELDLLDEAERRQILEGWNATHTGYPLARLVHQLIE 1581

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2614 AQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVE 2693
Cdd:PRK12467 1582 DQAAATPEAVALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRE 1661

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2694 RLRYMLDDAKPVALISQSA---HLGIMNGsLPVILLDDGETRpFDNEPDTPLDARkqgLTPRHLAYVIYTSGSTGKPKGV 2770
Cdd:PRK12467 1662 RLAYMIEDSGIELLLTQSHlqaRLPLPDG-LRSLVLDQEDDW-LEGYSDSNPAVN---LAPQNLAYVIYTSGSTGRPKGA 1736

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2771 MVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQAT 2850
Cdd:PRK12467 1737 GNRHGALVNRLCATQEAYQLSAADVVLQFTSFAFDVSVWELFWPLINGARLVIAPPGAHRDPEQLIQLIERQQVTTLHFV 1816

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2851 PSTWRMLVEL--RDFALPPGFKALCGGEALPENLATALLQKV--TTLWNLYGPTETTIWSTLNGLTTPTPY------IGH 2920
Cdd:PRK12467 1817 PSMLQQLLQMdeQVEHPLSLRRVVCGGEALEVEALRPWLERLpdTGLFNLYGPTETAVDVTHWTCRRKDLEgrdsvpIGQ 1896

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2921 PIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGR 3000
Cdd:PRK12467 1897 PIANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPF-GTVGSRLYRTGDLARYRADGVIEYLGR 1975

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3001 NDFQVKVRGFRIELGEIETRLARCHGVHDAVVIArEDSPGDKRLVAYLLAQPDTVLEPAD--------LRQRLSEGVAEY 3072
Cdd:PRK12467 1976 IDHQVKIRGFRIELGEIEARLREQGGVREAVVIA-QDGANGKQLVAYVVPTDPGLVDDDEaqvalraiLKNHLKASLPEY 2054

                        2090      2100      2110      2120      2130      2140      2150      2160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3073 MIPSAFVTLDAFPLTPNGKLDRKALPAPDQSAMaTRGYEAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQ 3152
Cdd:PRK12467 2055 MVPAHLVFLARMPLTPNGKLDRKALPAPDASEL-QQAYVAPQSELEQRLAAIWQDVLGLEQVGLHDNFFELGGDSIISIQ 2133

                        2170      2180      2190
                  ....*....|....*....|....*....|
gi 641744967 3153 LNARIRAEFLTDIPiVAIFQHPQLSALAEV 3182
Cdd:PRK12467 2134 VVSRARQAGIRFTP-KDLFQHQTVQSLAAV 2162
PRK12316 PRK12316
peptide synthase; Provisional
 2-2450 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 1720.18  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    2 PLVTLSQEEIDTViaTVPDGvaNVQDIYPLAPLQEGILFHYQLQEKGDTYLlnsllafdSQTRLD-------AFLDVLQQ 74
Cdd:PRK12316 1534 PLAGLSQAQLDAL--PLPAG--EIADIYPLSPMQQGMLFHSLYEQEAGDYI--------NQLRVDvqgldpdRFRAAWQA 1601

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   75 VIARHDILRTAICWQ-GLHQPVQVVWRQAPLTVNTLTTTSSDTVPAQLRAATDPSNHR-LNLSNAPLLSATTAHDPVcGE 152
Cdd:PRK12316 1602 TVDRHEILRSGFLWQdGLEQPLQVIHKQVELPFAELDWRGREDLGQALDALAQAERQKgFDLTRAPLLRLVLVRTGE-GR 1680

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  153 WLLSLSIHHLISDHITQALIIDEIRLLLEDRPEALPKpLPYRNFIAQILSVPLSEHEQYFRNRLADIDTPTAPFDLVDVQ 232
Cdd:PRK12316 1681 HHLIYTNHHILMDGWSNAQLLGEVLQRYAGQPVAAPG-GRYRDYIAWLQRQDAAASEAFWKEQLAALEEPTRLAQAARTE 1759

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  233 GNGEDITEARLSLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGADRVMGMFINTL 312
Cdd:PRK12316 1760 DGQVGYGDHQQLLDPAQTRALAEFARAQKVTLNTLVQAAWLLLLQRYTGQETVAFGATVAGRPAELPGIEQQIGLFINTL 1839

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  313 PLRVSLR-ERSVHDVVQATSHELMMLLAHEQAPLALAQQCSQVPPPlPLFSTLF---NYRHSQ-----KDASSQFWEgmr 383
Cdd:PRK12316 1840 PVIAAPRpDQSVADWLQEVQALNLALREHEHTPLYDIQRWAGQGGE-ALFDSLLvfeNYPVAEalkqgAPAGLVFGR--- 1915

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  384 qLSGRERTNYPITLSVDdLGDGFNLTAKTVMG----VDPERIVHYMLtaieNLVTSLEKTPQQPALSQPILPKSERQQVL 459
Cdd:PRK12316 1916 -VSNHEQTNYPLTLAVT-LGETLSLQYSYDRGhfdaAAIERLDRHLL----HLLEQMAEDAQAALGELALLDAGERQRIL 1989

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  460 VDFNATDADFPREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVG 539
Cdd:PRK12316 1990 ADWDRTPEAYPRGPGVHQRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVA 2069

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  540 LLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLNST--LPTVLLDTPAA-AACPDTNPVVQgLHAAHL 616
Cdd:PRK12316 2070 LLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLTQRHLLERLPLPagVARLPLDRDAEwADYPDTAPAVQ-LAGENL 2148

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  617 AYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQ-LLSGHTLVLVPDALRaDAHQ 695
Cdd:PRK12316 2149 AYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSFSFDGAHEQWFHpLLNGARVLIRDDELW-DPEQ 2227

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  696 LWRYFARHAVDLFDCTPVQLQWLLD--AGLGSDPAYQpaQVLIGGEAISPAVWSR-LQSLSDTRFINVYGPTECTVDATA 772
Cdd:PRK12316 2228 LYDEMERHGVTILDFPPVYLQQLAEhaERDGRPPAVR--VYCFGGEAVPAASLRLaWEALRPVYLFNGYGPTEAVVTPLL 2305

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  773 CVVDRTQP----LPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSAdPAARIYK 848
Cdd:PRK12316 2306 WKCRPQDPcgaaYVPIGRALGNRRAYILDADLNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPDPFSA-SGERLYR 2384

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  849 TGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAReDSPGDTRLVAYLCARPDAELHPAALR 928
Cdd:PRK12316 2385 TGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVVAQ-DGASGKQLVAYVVPDDAAEDLLAELR 2463

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  929 QQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAfATRDYEAPQGGIETALAALWQELLGLDRVGRHDQFFA 1008
Cdd:PRK12316 2464 AWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQ-LRQAYVAPQEGLEQRLAAIWQAVLKVEQVGLDDHFFE 2542

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1009 LGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAVTATAHDAPFTLPVADRTQPLPLSFSQQRLWFLAQLDPAa 1087
Cdd:PRK12316 2543 LGGHSLLATQVVSRVRQDlGLEVPLRILFERPTLAAFAASLESGQTSRAPVLQKVTRVQPLPLSHAQQRQWFLWQLEPE- 2621

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1088 SQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLGFSLSSHDLRKLDEAARttrvaELA 1167
Cdd:PRK12316 2622 SAAYHLPSALHLRGVLDQAALEQAFDALVLRHETLRTRFVEVGEQTRQVILPNMSLRIVLEDCAGVADAAIR-----QRV 2696

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1168 EQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQYADYAVWQ 1247
Cdd:PRK12316 2697 AEEIQRPFDLARGPLLRVRLLALDGQEHVLVITQHHIVSDGWSMQVMVDELVQAYAGARRGEQPTLPPLPLQYADYAAWQ 2776

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1248 RQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHALNRQQGTTLFMTLLAAWSVV 1327
Cdd:PRK12316 2777 RAWMDSGEGARQLDYWRERLGGEQPVLELPLDRPRPALQSHRGARLDVALDVALSRELLALARREGVTLFMLLLASFQVL 2856

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1328 LSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALDAYAHQALPFEQVVEILQPAR 1407
Cdd:PRK12316 2857 LHRYSGQSDIRVGVPIANRNRAETERLIGFFVNTQVLRAQVDAQLAFRDLLGQVKEQALGAQAHQDLPFEQLVEALQPER 2936

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1408 SLSYSPIFQVMLSLNNTPAQALTLPDLTLSAVERPQHSTHFDLSLSLIETENGLNGGLVYATDLFDRETILRVVGYVENI 1487
Cdd:PRK12316 2937 SLSHSPLFQVMYNHQSGERAAAQLPGLHIESFAWDGAATQFDLALDTWESAEGLGASLTYATDLFDARTVERLARHWQNL 3016

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1488 LMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLA 1567
Cdd:PRK12316 3017 LRGMVENPQRSVDELAMLDAEERGQLLEAWNATAAEYPLERGVHRLFEEQVERTPDAVALAFGEQRLSYAELNRRANRLA 3096

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1568 HHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQANQRALLTGDVPRIL 1647
Cdd:PRK12316 3097 HRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLLSQSHLRLPLAQGVQVLD 3176

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1648 LDtADFSHLSEDNPHVpGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWE 1727
Cdd:PRK12316 3177 LD-RGDENYAEANPAI-RTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQAYGLGVGDRVLQFTTFSFDVFVEE 3254

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1728 FFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADcACDSLRRVICSGEALPAELQQRFFA 1807
Cdd:PRK12316 3255 LFWPLMSGARVVLAGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAH-RCTSLKRIVCGGEALPADLQQQVFA 3333

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1808 RfnAQLHNLYGPTEAAIDVTFWACqPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLT 1887
Cdd:PRK12316 3334 G--LPLYNLYGPTEATITVTHWQC-VEEGKDAVPIGRPIANRACYILDGSLEPVPVGALGELYLGGEGLARGYHNRPGLT 3410

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1888 AERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSpgdtR 1967
Cdd:PRK12316 3411 AERFVPDPF--VPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVLAVDGR----Q 3484

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1968 LVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAPDqSAVATRDYEAPQGEVETALAA 2047
Cdd:PRK12316 3485 LVAYVVPEDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPD-AALLQQDYVAPVNELERRLAA 3563

                        2090      2100      2110      2120      2130      2140      2150      2160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2048 VWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGLALDVRGVFSTPVLSDMAQAIlahqdkpavvvppnripadst 2127
Cdd:PRK12316 3564 IWADVLKLEQVGLTDNFFELGGDSIISLQVVSRARQAGIRFTPKDLFQHQTIQGLARVA--------------------- 3622

                        2170      2180      2190      2200      2210      2220      2230      2240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2128 aitpdllplvtltqpeidRITDTVSGGASNIQDIYPLAPLQEGIL------FHHLLQEQgdtyLLRSmvafthRERLD-- 2199
Cdd:PRK12316 3623 ------------------RVGGGVAVDQGPVSGETLLLPIQQQFFeepvpeRHHWNQSL----LLKP------REALDaa 3674

                        2250      2260      2270      2280      2290      2300      2310      2320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2200 AFLSALQQVIDRHDILRTAVcwqdlsQPVQVVWRQAILPInhfepTSPEDVL--------AQLQAHTEPRTRRIDLSQAP 2271
Cdd:PRK12316 3675 ALEAALQALVEHHDALRLRF------VEDAGGWTAEHLPV-----ELGGALLwraelddaEELERLGEEAQRSLDLADGP 3743

                        2330      2340      2350      2360      2370      2380      2390      2400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2272 LFRADIAHDPLQNEWLLaLSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLPYRN--FIAQTLSVPNSAHE------- 2342
Cdd:PRK12316 3744 LLRALLATLADGSQRLL-LVIHHLVVDGVSWRILLEDLQQAYQQLLQGEAPRLPAKTssFKAWAERLQEHARGealkael 3822

                        2410      2420      2430      2440      2450      2460      2470      2480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2343 AYFRDKLADVdepTAPFGLLNVQGSGGDIHEARLV--LDATLASAIRQQA-RHLGVSPGVLFHVAWAQVLAQTSGRDDVV 2419
Cdd:PRK12316 3823 AYWQEQLQGV---SSELPCDHPQGALQNRHAASVQtrLDRELTRRLLQQApAAYRTQVNDLLLTALARVVCRWTGEASAL 3899

                        2490      2500      2510
                  ....*....|....*....|....*....|...
gi 641744967 2420 FGSVLLGRLAGAEGAD--RIMGMFINTLPLRIS 2450
Cdd:PRK12316 3900 VQLEGHGREDLFADIDlsRTVGWFTSLFPVRLS 3932
PRK05691 PRK05691
peptide synthase; Validated
 465-3191 0e+00

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 1668.39  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  465 TDAdFPREMLIQQRFEAQAEQTPEAIAVLF------EDQHLTYRELNRRANQLAHHLIALGvQPDDRVALCVERSLEMMV 538
Cdd:PRK05691    2 MDA-FELPLTLVQALQRRAAQTPDRLALRFladdpgEGVVLSYRDLDLRARTIAAALQARA-SFGDRAVLLFPSGPDYVA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  539 GLLGILKAGAAYVPmdpAYPAE--------RLAYILDDAAPVALLTQSA---QVAQLN----STLPTVL-LDT-PAAAAC 601
Cdd:PRK05691   80 AFFGCLYAGVIAVP---AYPPEsarrhhqeRLLSIIADAEPRLLLTVADlrdSLLQMEelaaANAPELLcVDTlDPALAE 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  602 PDTNPVVQGlhaAHLAYVIYTSGSTGRPKGVMVAHRNVINlatglhTLLALDHPSRIALNAsivfDASVKNWIQLLsgHT 681
Cdd:PRK05691  157 AWQEPALQP---DDIAFLQYTSGSTALPKGVQVSHGNLVA------NEQLIRHGFGIDLNP----DDVIVSWLPLY--HD 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  682 LVL-----------VPDALRADAHQLWRyfarhavdlfdctpvQLQWLLDAGL-------GSDPAYQPAQVLIGGEAISP 743
Cdd:PRK05691  222 MGLiggllqpifsgVPCVLMSPAYFLER---------------PLRWLEAISEyggtisgGPDFAYRLCSERVSESALER 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  744 AVWS------------RLQSL------------SDTRFINVYGPTECT----------------VDATACVVDRTQP--- 780
Cdd:PRK05691  287 LDLSrwrvaysgsepiRQDSLerfaekfaacgfDPDSFFASYGLAEATlfvsggrrgqgipaleLDAEALARNRAEPgtg 366

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  781 --LPTIGKPLANTRLYILDAQDQPV-PIGVTGELHIGGAGVARGYLHRPDLTAERFIpdpfsaDPAARIY-KTGDLArWL 856
Cdd:PRK05691  367 svLMSCGRSQPGHAVLIVDPQSLEVlGDNRVGEIWASGPSIAHGYWRNPEASAKTFV------EHDGRTWlRTGDLG-FL 439

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  857 PDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGV---------------QDAVVIAREDSPGDTRLVAylcarPDAE 921
Cdd:PRK05691  440 RDGELFVTGRLKDMLIVRGHNLYPQDIEKTVEREVEVvrkgrvaafavnhqgEEGIGIAAEISRSVQKILP-----PQAL 514

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  922 LHpaALRQQLAASLADymIPSAFVTLD--ALPLTPNGKLDRKA---------------LPAPDQTAfaTRDYEAPQGGIE 984
Cdd:PRK05691  515 IK--SIRQAVAEACQE--APSVVLLLNpgALPKTSSGKLQRSAcrlrladgsldsyalFPALQAVE--AAQTAASGDELQ 588

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  985 TALAALWQELLGLDRVGRHDQFFALGGHSLLAVQLLNRM-NKAGMDVALATLFAHPTLCDLAAAVTATAHD---APFTLP 1060
Cdd:PRK05691  589 ARIAAIWCEQLKVEQVAADDHFFLLGGNSIAATQVVARLrDELGIDLNLRQLFEAPTLAAFSAAVARQLAGggaAQAAIA 668

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1061 VADRTQPLPLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPd 1140
Cdd:PRK05691  669 RLPRGQALPQSLAQNRLWLLWQLDPQSA-AYNIPGGLHLRGELDEAALRASFQRLVERHESLRTRFYERDGVALQRIDA- 746

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1141 TLGFSLSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAA 1220
Cdd:PRK05691  747 QGEFALQRIDLSDLPEAEREARAAQIREEEARQPFDLEKGPLLRVTLVRLDDEEHQLLVTLHHIVADGWSLNILLDEFSR 826

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1221 LYQAALEGSEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAG 1300
Cdd:PRK05691  827 LYAAACQGQTAELAPLPLGYADYGAWQRQWLAQGEAARQLAYWKAQLGDEQPVLELATDHPRSARQAHSAARYSLRVDAS 906

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1301 QLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQ 1380
Cdd:PRK05691  907 LSEALRGLAQAHQATLFMVLLAAFQALLHRYSGQGDIRIGVPNANRPRLETQGLVGFFINTQVLRAQLDGRLPFTALLAQ 986

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1381 VRERALDAYAHQALPFEQVVEILQPARSlsySPIFQVMLSLNNTPAQAL-TLPDLTlsAVERPQHSTH--FDLSLSLIET 1457
Cdd:PRK05691  987 VRQATLGAQAHQDLPFEQLVEALPQARE---QGLFQVMFNHQQRDLSALrRLPGLL--AEELPWHSREakFDLQLHSEED 1061

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1458 ENG-LNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQvMLDFNATEADFPHDALIHQLvED 1536
Cdd:PRK05691 1062 RNGrLTLSFDYAAELFDAATIERLAEHFLALLEQVCEDPQRALGDVQLLDAAERAQ-LAQWGQAPCAPAQAWLPELL-NE 1139

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1537 QAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAER 1616
Cdd:PRK05691 1140 QARQTPERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPDYPAER 1219

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1617 LAYMLDDASPVALLTQANqralLTGDVPR------ILLDTAdfsHLSEDNPHVPGLDAH--HLAYVIYTSGSTGKPKGVM 1688
Cdd:PRK05691 1220 LAYMLADSGVELLLTQSH----LLERLPQaegvsaIALDSL---HLDSWPSQAPGLHLHgdNLAYVIYTSGSTGQPKGVG 1292

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1689 NSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVP 1768
Cdd:PRK05691 1293 NTHAALAERLQWMQATYALDDSDVLMQKAPISFDVSVWECFWPLITGCRLVLAGPGEHRDPQRIAELVQQYGVTTLHFVP 1372

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1769 SMLQQFVQWADAdCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDDHrSFVPIGRPIA 1847
Cdd:PRK05691 1373 PLLQLFIDEPLA-AACTSLRRLFSGGEALPAELRNRVLQRLpQVQLHNRYGPTETAINVTHWQCQAEDG-ERSPIGRPLG 1450

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1848 NTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDF 1927
Cdd:PRK05691 1451 NVLCRVLDAELNLLPPGVAGELCIGGAGLARGYLGRPALTAERFVPDPL-GEDGARLYRTGDRARWNADGALEYLGRLDQ 1529

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1928 QVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGdTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDA 2007
Cdd:PRK05691 1530 QVKLRGFRVEPEEIQARLLAQPGVAQAAVLVREGAAG-AQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQ 1608

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2008 FPLTPNGKLDRKALPAPDQSavaTRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRA-GL 2086
Cdd:PRK05691 1609 MPLGPSGKLDRRALPEPVWQ---QREHVEPRTELQQQIAAIWREVLGLPRVGLRDDFFALGGHSLLATQIVSRTRQAcDV 1685

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2087 ALDVRGVFSTPVLSDMAQAILAHQDKPAvvvppnripadstaitpdllplvTLTQPEIDRITDTvsggasniQDIyPLAP 2166
Cdd:PRK05691 1686 ELPLRALFEASELGAFAEQVARIQAAGE-----------------------RNSQGAIARVDRS--------QPV-PLSY 1733

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2167 LQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRT------AVCWQDLSQP--VQVVWRQailp 2238
Cdd:PRK05691 1734 SQQRMWFLWQMEPDSPAYNVGGMARLSGVLDVDRFEAALQALILRHETLRTtfpsvdGVPVQQVAEDsgLRMDWQD---- 1809

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2239 INHFEPTSPEDVLAQL---QAHteprtRRIDLSQAPLFRADIAHDPLQnEWLLALSFHHLISDHMTLALIVGEIRLLLQH 2315
Cdd:PRK05691 1810 FSALPADARQQRLQQLadsEAH-----QPFDLERGPLLRACLVKAAER-EHYFVLTLHHIVTEGWAMDIFARELGALYEA 1883

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2316 QADALPTPLP--------YRNFIAQTLSVPNSAHE-AYFRDKLAD--------VDEPTAPfgllnVQGSGGDIHeaRLVL 2378
Cdd:PRK05691 1884 FLDDRESPLEplpvqyldYSVWQRQWLESGERQRQlDYWKAQLGNehpllelpADRPRPP-----VQSHRGELY--RFDL 1956

                        2090      2100      2110      2120      2130      2140      2150      2160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2379 DATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRL-AGAEGadrIMGMFINTLPLRISL-ADRGA 2456
Cdd:PRK05691 1957 SPELAARVRAFNAQRGLTLFMTMTATLAALLYRYSGQRDLRIGAPVANRIrPESEG---LIGAFLNTQVLRCQLdGQMSV 2033

                        2170      2180      2190      2200      2210      2220      2230      2240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2457 AEVVERTSHDLMTLLEHEQAPlaLAQRCSGVAPPM-----PLFSTLLNYRHTQASSTdNTLSDIRV--LTSEER-TNYPL 2528
Cdd:PRK05691 2034 SELLEQVRQTVIEGQSHQDLP--FDHLVEALQPPRsaaynPLFQVMCNVQRWEFQQS-RQLAGMTVeyLVNDARaTKFDL 2110

                        2250      2260      2270      2280      2290      2300      2310      2320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2529 TLAVDDRGEGFSLVAQTLEDIDPHRLLNYLMTAISSLVDALETEPQRSILNLPVLPDSERQQMLVDFNATDADIPRHALI 2608
Cdd:PRK05691 2111 NLEVTDLDGRLGCCLTYSRDLFDEPRIARMAEHWQNLLEALLGDPQQRLAELPLLAAAEQQQLLDSLAGEAGEARLDQTL 2190

                        2330      2340      2350      2360      2370      2380      2390      2400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2609 HELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDP 2688
Cdd:PRK05691 2191 HGLFAAQAARTPQAPALTFAGQTLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDP 2270

                        2410      2420      2430      2440      2450      2460      2470      2480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2689 TYPVERLRYMLDDAKPVALISQSAHLGIMnGSLPV-----ILLDDGETrpFDNEPDTPLDARKQgltPRHLAYVIYTSGS 2763
Cdd:PRK05691 2271 EYPLERLHYMIEDSGIGLLLSDRALFEAL-GELPAgvarwCLEDDAAA--LAAYSDAPLPFLSL---PQHQAYLIYTSGS 2344

                        2490      2500      2510      2520      2530      2540      2550      2560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2764 TGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAmLIERHA 2843
Cdd:PRK05691 2345 TGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSINFDAASERLLVPLLCGARVVLRAQGQWGAEEICQ-LIREQQ 2423

                        2570      2580      2590      2600      2610      2620      2630      2640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2844 VSFMQATPSTWRML---VELRDFALPpgfKALC--GGEAL-PENLA--TALLQKvTTLWNLYGPTETTIW-------STL 2908
Cdd:PRK05691 2424 VSILGFTPSYGSQLaqwLAGQGEQLP---VRMCitGGEALtGEHLQriRQAFAP-QLFFNAYGPTETVVMplaclapEQL 2499

                        2650      2660      2670      2680      2690      2700      2710      2720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 NGLTTPTPyIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSgAPEARMYKTGDLGR 2988
Cdd:PRK05691 2500 EEGAASVP-IGRVVGARVAYILDADLALVPQGATGELYVGGAGLAQGYHDRPGLTAERFVADPFA-ADGGRLYRTGDLVR 2577

                        2730      2740      2750      2760      2770      2780      2790      2800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2989 WLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAReDSPGDKRLVAYLLAQPDTVLEPAD------LR 3062
Cdd:PRK05691 2578 LRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVLAL-DTPSGKQLAGYLVSAVAGQDDEAQaalreaLK 2656

                        2810      2820      2830      2840      2850      2860      2870      2880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3063 QRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPDqSAMATRGYEAPQGDLEHALAQIWQTLLGVERVGRHDHFFE 3142
Cdd:PRK05691 2657 AHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRALPAPD-PELNRQAYQAPRSELEQQLAQIWREVLNVERVGLGDNFFE 2735

                        2890      2900      2910      2920
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967 3143 LGGHSLLAVQLNARIRAEFLTDIPiVAIFQHPQLSALAEVILAAQIHAA 3191
Cdd:PRK05691 2736 LGGDSILSIQVVSRARQLGIHFSP-RDLFQHQTVQTLAAVATHSEAAQA 2783
PRK05691 PRK05691
peptide synthase; Validated
 30-2461 0e+00

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 1478.49  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAIcWQGLHQPVQVVWRQAPLTVNTL 109
Cdd:PRK05691  677 PQSLAQNRLWLLWQLDPQSAAYNIPGGLHLRGELDEAALRASFQRLVERHESLRTRF-YERDGVALQRIDAQGEFALQRI 755

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  110 TTTSSDTVPAQLRAAT---DPSNHRLNLSNAPLLSATTAH--DPvcgEWLLSLSIHHLISDHITQALIIDEIRLLLEDRP 184
Cdd:PRK05691  756 DLSDLPEAEREARAAQireEEARQPFDLEKGPLLRVTLVRldDE---EHQLLVTLHHIVADGWSLNILLDEFSRLYAAAC 832

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  185 EALP---KPLP-----YRNFIAQILSVPLSEHE-QYFRNRLADiDTPTAPFDLVDVQGNGEDITEARLSL--DSSLADAL 253
Cdd:PRK05691  833 QGQTaelAPLPlgyadYGAWQRQWLAQGEAARQlAYWKAQLGD-EQPVLELATDHPRSARQAHSAARYSLrvDASLSEAL 911

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  254 RRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQgnLGADRVMGMFINTLPLRVSLRER-SVHDVVQATSH 332
Cdd:PRK05691  912 RGLAQAHQATLFMVLLAAFQALLHRYSGQGDIRIGVPNANRPR--LETQGLVGFFINTQVLRAQLDGRlPFTALLAQVRQ 989

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  333 ELMMLLAHEQAPLALAQQCSQVPPPLPLFSTLFNyrHSQKDASSQF-----------WEGMR-----QLSGRERTNYPIT 396
Cdd:PRK05691  990 ATLGAQAHQDLPFEQLVEALPQAREQGLFQVMFN--HQQRDLSALRrlpgllaeelpWHSREakfdlQLHSEEDRNGRLT 1067

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  397 LSVDDLGDGFNltAKTVmgvdpERIVHYMLtaieNLVTSLEKTPQQPALSQPILPKSERQQvLVDFNATDADfPREMLIQ 476
Cdd:PRK05691 1068 LSFDYAAELFD--AATI-----ERLAEHFL----ALLEQVCEDPQRALGDVQLLDAAERAQ-LAQWGQAPCA-PAQAWLP 1134

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  477 QRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPA 556
Cdd:PRK05691 1135 ELLNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPD 1214

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  557 YPAERLAYILDDAAPVALLTQSAQVAQLNST--LPTVLLDTPAAAACPDTNPVVQgLHAAHLAYVIYTSGSTGRPKGVMV 634
Cdd:PRK05691 1215 YPAERLAYMLADSGVELLLTQSHLLERLPQAegVSAIALDSLHLDSWPSQAPGLH-LHGDNLAYVIYTSGSTGQPKGVGN 1293

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  635 AHRNVINLATGLHTLLALDHPSRIALNASIVFDASV-KNWIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPV 713
Cdd:PRK05691 1294 THAALAERLQWMQATYALDDSDVLMQKAPISFDVSVwECFWPLITGCRLVLAGPGEHRDPQRIAELVQQYGVTTLHFVPP 1373

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  714 QLQWLLDAGLGSDpAYQPAQVLIGGEAISPAVWSR-LQSLSDTRFINVYGPTECTVDAT--ACVVDRTQPLPtIGKPLAN 790
Cdd:PRK05691 1374 LLQLFIDEPLAAA-CTSLRRLFSGGEALPAELRNRvLQRLPQVQLHNRYGPTETAINVThwQCQAEDGERSP-IGRPLGN 1451

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  791 TRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSaDPAARIYKTGDLARWLPDGNIDYLGRNDFQ 870
Cdd:PRK05691 1452 VLCRVLDAELNLLPPGVAGELCIGGAGLARGYLGRPALTAERFVPDPLG-EDGARLYRTGDRARWNADGALEYLGRLDQQ 1530

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  871 IKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGdTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDAL 950
Cdd:PRK05691 1531 VKLRGFRVEPEEIQARLLAQPGVAQAAVLVREGAAG-AQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQM 1609

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  951 PLTPNGKLDRKALPAPDqtaFATRDYEAPQGGIETALAALWQELLGLDRVGRHDQFFALGGHSLLAVQLLNRMNKA-GMD 1029
Cdd:PRK05691 1610 PLGPSGKLDRRALPEPV---WQQREHVEPRTELQQQIAAIWREVLGLPRVGLRDDFFALGGHSLLATQIVSRTRQAcDVE 1686

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1030 VALATLFAHPTLCDLAAAV----TATAHDAPFTLPVADRTQPLPLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDR 1105
Cdd:PRK05691 1687 LPLRALFEASELGAFAEQVariqAAGERNSQGAIARVDRSQPVPLSYSQQRMWFLWQMEPD-SPAYNVGGMARLSGVLDV 1765

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1106 PALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTlGFSLSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRG 1185
Cdd:PRK05691 1766 DRFEAALQALILRHETLRTTFPSVDGVPVQQVAEDS-GLRMDWQDFSALPADARQQRLQQLADSEAHQPFDLERGPLLRA 1844

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1186 QLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQYADYAVWQRQWLQ-GETLNDLrDYWR 1264
Cdd:PRK05691 1845 CLVKAAEREHYFVLTLHHIVTEGWAMDIFARELGALYEAFLDDRESPLEPLPVQYLDYSVWQRQWLEsGERQRQL-DYWK 1923

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1265 DQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVA 1344
Cdd:PRK05691 1924 AQLGNEHPLLELPADRPRPPVQSHRGELYRFDLSPELAARVRAFNAQRGLTLFMTMTATLAALLYRYSGQRDLRIGAPVA 2003

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1345 NRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNT 1424
Cdd:PRK05691 2004 NRIRPESEGLIGAFLNTQVLRCQLDGQMSVSELLEQVRQTVIEGQSHQDLPFDHLVEALQPPRSAAYNPLFQVMCNVQRW 2083

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1425 P-AQALTLPDLTLSAVERPQHSTHFDLSLSLIETENGLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLP 1503
Cdd:PRK05691 2084 EfQQSRQLAGMTVEYLVNDARATKFDLNLEVTDLDGRLGCCLTYSRDLFDEPRIARMAEHWQNLLEALLGDPQQRLAELP 2163

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1504 ILPDSERRQVMLDFNATEADFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAI 1583
Cdd:PRK05691 2164 LLAAAEQQQLLDSLAGEAGEARLDQTLHGLFAAQAARTPQAPALTFAGQTLSYAELDARANRLARALRERGVGPQVRVGL 2243

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1584 CVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTqanQRALLT--GDVP----RILL--DTADFSH 1655
Cdd:PRK05691 2244 ALERSLEMVVGLLAILKAGGAYVPLDPEYPLERLHYMIEDSGIGLLLS---DRALFEalGELPagvaRWCLedDAAALAA 2320

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1656 LSEDNPHVPGLdAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYG 1735
Cdd:PRK05691 2321 YSDAPLPFLSL-PQHQAYLIYTSGSTGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSINFDAASERLLVPLLCG 2399

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1736 ARLVMaRPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARFN-AQLH 1814
Cdd:PRK05691 2400 ARVVL-RAQGQWGAEEICQLIREQQVSILGFTPSYGSQLAQWLAGQGEQLPVRMCITGGEALTGEHLQRIRQAFApQLFF 2478

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1815 NLYGPTEAAidVTFWAC----QPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAER 1890
Cdd:PRK05691 2479 NAYGPTETV--VMPLAClapeQLEEGAASVPIGRVVGARVAYILDADLALVPQGATGELYVGGAGLAQGYHDRPGLTAER 2556

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1891 FIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAReDSPGDTRLVA 1970
Cdd:PRK05691 2557 FVADPF-AADGGRLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVLAL-DTPSGKQLAG 2634

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1971 YL-CPQPGVTPDPAD-----LRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAPDqSAVATRDYEAPQGEVETA 2044
Cdd:PRK05691 2635 YLvSAVAGQDDEAQAalreaLKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRALPAPD-PELNRQAYQAPRSELEQQ 2713

                        2090      2100      2110      2120      2130      2140      2150      2160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2045 LAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGLALDVRGVFSTPVLSDMAqailahqdkpAVVvppnripa 2124
Cdd:PRK05691 2714 LAQIWREVLNVERVGLGDNFFELGGDSILSIQVVSRARQLGIHFSPRDLFQHQTVQTLA----------AVA-------- 2775

                        2170      2180      2190      2200      2210      2220      2230      2240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2125 dstaitpdllplvtltqpeidRITDTVSGGASNIQDIYPLAPLQEGILFHHLLQEQ--GDTYLLRSmvafthRERLDAFL 2202
Cdd:PRK05691 2776 ---------------------THSEAAQAEQGPLQGASGLTPIQHWFFDSPVPQPQhwNQALLLEP------RQALDPAL 2828

                        2250      2260      2270      2280      2290      2300      2310      2320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2203 --SALQQVIDRHDILRTAVC-----WQDLSQPV---QVVWrqailpinHFEPTSPEDVLAqLQAHTEprtRRIDLSQAPL 2272
Cdd:PRK05691 2829 leQALQALVEHHDALRLRFSqadgrWQAEYRAVtaqELLW--------QVTVADFAECAA-LFADAQ---RSLDLQQGPL 2896

                        2330      2340      2350      2360      2370      2380      2390      2400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2273 FRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLP-----YRNFIAQTLSVPNSAHeayFRD 2347
Cdd:PRK05691 2897 LRALLVDGP-QGQQRLLLAIHHLVVDGVSWRVLLEDLQALYRQLSAGAEPALPaktsaFRDWAARLQAYAGSES---LRE 2972

                        2410      2420      2430      2440      2450      2460      2470      2480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2348 KL----ADVDEPTAPFGLLNVQGSGGDIHeARLV---LDATLASAIRQQ---ARHLGVSPgvLFHVAWAQVLAQTSGRDD 2417
Cdd:PRK05691 2973 ELgwwqAQLGGPRAELPCDRPQGGNLNRH-AQTVsvrLDAERTRQLLQQapaAYRTQVND--LLLTALARVLCRWSGQPS 3049

                        2490      2500      2510      2520
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 2418 VVFGSVLLGRLAGAEGAD--RIMGMFINTLPLRISLADRGAAEVVE 2461
Cdd:PRK05691 3050 VLVQLEGHGREALFDDIDltRSVGWFTSAYPLRLTPAPGDDAARGE 3095
PRK12316 PRK12316
peptide synthase; Provisional
 1069-3182 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 1399.69  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1069 PLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGrLDRPALTTALNGLVARHESLRTRFTSIDG--QPAQQIDpDTLGFSL 1146
Cdd:PRK12316 1558 PLSPMQQGMLFHSLYEQEAG-DYINQLRVDVQG-LDPDRFRAAWQATVDRHEILRSGFLWQDGleQPLQVIH-KQVELPF 1634

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1147 SSHDLRKLDEAARTtrVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAal 1226
Cdd:PRK12316 1635 AELDWRGREDLGQA--LDALAQAERQKGFDLTRAPLLRLVLVRTGEGRHHLIYTNHHILMDGWSNAQLLGEVLQRYAG-- 1710

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1227 egseaNLPPLPV-QYADYAVWqrqwLQGETLNDLRDYWRDQLqgapALLEIPT---DRPRPSVQRYAGDQVPFHLDAGQL 1302
Cdd:PRK12316 1711 -----QPVAAPGgRYRDYIAW----LQRQDAAASEAFWKEQL----AALEEPTrlaQAARTEDGQVGYGDHQQLLDPAQT 1777

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1303 RRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPR--QELEGMVGFFVNTLALRTEPGRCHAVADLLDQ 1380
Cdd:PRK12316 1778 RALAEFARAQKVTLNTLVQAAWLLLLQRYTGQETVAFGATVAGRPAelPGIEQQIGLFINTLPVIAAPRPDQSVADWLQE 1857

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1381 VRERALDAYAHQALPFEQVveilQPARSLSYSPIFQVMLSLNNTP-AQAL---TLPDLTLSAVERpQHSTHFDLSLSlIE 1456
Cdd:PRK12316 1858 VQALNLALREHEHTPLYDI----QRWAGQGGEALFDSLLVFENYPvAEALkqgAPAGLVFGRVSN-HEQTNYPLTLA-VT 1931

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1457 TENGLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDALIHQLVED 1536
Cdd:PRK12316 1932 LGETLSLQYSYDRGHFDAAAIERLDRHLLHLLEQMAEDAQAALGELALLDAGERQRILADWDRTPEAYPRGPGVHQRIAE 2011

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1537 QAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAER 1616
Cdd:PRK12316 2012 QAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAER 2091

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1617 LAYMLDDASPVALLTQAN--QRALLTGDVPRILLDT-ADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMNSHRA 1693
Cdd:PRK12316 2092 LAYMLEDSGAALLLTQRHllERLPLPAGVARLPLDRdAEWADYPDTAPAVQ-LAGENLAYVIYTSGSTGLPKGVAVSHGA 2170

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1694 LCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMaRPDGHKDAAYLAQLIERTGITTLHFVPSMLQQ 1773
Cdd:PRK12316 2171 LVAHCQAAGERYELSPADCELQFMSFSFDGAHEQWFHPLLNGARVLI-RDDELWDPEQLYDEMERHGVTILDFPPVYLQQ 2249

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1774 FVQWADADCACDSLRRVICSGEALPAELQQRFFARFNAQ-LHNLYGPTEAAIDVTFWACQPDD--HRSFVPIGRPIANTQ 1850
Cdd:PRK12316 2250 LAEHAERDGRPPAVRVYCFGGEAVPAASLRLAWEALRPVyLFNGYGPTEAVVTPLLWKCRPQDpcGAAYVPIGRALGNRR 2329

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1851 LYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINqPGARLYKTGDLARWLPDGSLEYLGRNDFQVK 1930
Cdd:PRK12316 2330 AYILDADLNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPDPFSA-SGERLYRTGDLARYRADGVVEYLGRIDHQVK 2408

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1931 LRGFRIELGEIEARLMQCPGVQEAVVVAReDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPL 2010
Cdd:PRK12316 2409 IRGFRIELGEIEARLQAHPAVREAVVVAQ-DGASGKQLVAYVVPDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPL 2487

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2011 TPNGKLDRKALPAPDQSAvATRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRA-GLALD 2089
Cdd:PRK12316 2488 NPNGKLDRKALPKPDVSQ-LRQAYVAPQEGLEQRLAAIWQAVLKVEQVGLDDHFFELGGHSLLATQVVSRVRQDlGLEVP 2566

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2090 VRGVFSTPVLSDMAQAILAHQdkpavvvppnripadsTAITPDLLPlVTLTQPeidritdtvsggasniqdiYPLAPLQE 2169
Cdd:PRK12316 2567 LRILFERPTLAAFAASLESGQ----------------TSRAPVLQK-VTRVQP-------------------LPLSHAQQ 2610

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2170 GILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSqpvqvvWRQAILPINHFEPTSPED 2249
Cdd:PRK12316 2611 RQWFLWQLEPESAAYHLPSALHLRGVLDQAALEQAFDALVLRHETLRTRFVEVGEQ------TRQVILPNMSLRIVLEDC 2684

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2250 VL----AQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLaLSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLP 2325
Cdd:PRK12316 2685 AGvadaAIRQRVAEEIQRPFDLARGPLLRVRLLALDGQEHVLV-ITQHHIVSDGWSMQVMVDELVQAYAGARRGEQPTLP 2763

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2326 --------YRNFIAQTLSVPNSAHE-AYFRDKLADVDePTAPFGLLNVQGSGGDIHEARLV--LDATLASAIRQQARHLG 2394
Cdd:PRK12316 2764 plplqyadYAAWQRAWMDSGEGARQlDYWRERLGGEQ-PVLELPLDRPRPALQSHRGARLDvaLDVALSRELLALARREG 2842

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2395 VSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISLADRGA-AEVVERTSHDLMTLLEH 2473
Cdd:PRK12316 2843 VTLFMLLLASFQVLLHRYSGQSDIRVGVPIANR--NRAETERLIGFFVNTQVLRAQVDAQLAfRDLLGQVKEQALGAQAH 2920

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2474 EQAP---LALAQRCSGVAPPMPLFSTLLNyrHTQASSTDNTLSDIRVLT-----SEERTNYPLTLAVDDRGEGFSLVAQT 2545
Cdd:PRK12316 2921 QDLPfeqLVEALQPERSLSHSPLFQVMYN--HQSGERAAAQLPGLHIESfawdgAATQFDLALDTWESAEGLGASLTYAT 2998

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2546 leDIDPHRLLNYLMTAISSLVDALETEPQRSILNLPVLPDSERQQMLVDFNATDADIPRHALIHELFEAQVACTPDAIAV 2625
Cdd:PRK12316 2999 --DLFDARTVERLARHWQNLLRGMVENPQRSVDELAMLDAEERGQLLEAWNATAAEYPLERGVHRLFEEQVERTPDAVAL 3076

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2626 VFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPV 2705
Cdd:PRK12316 3077 AFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQ 3156

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2706 ALISQSAHLGIMNGSLPVILLDDGETRPFDNEPDTpldarkqGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMR 2785
Cdd:PRK12316 3157 LLLSQSHLRLPLAQGVQVLDLDRGDENYAEANPAI-------RTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQ 3229

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2786 KEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELRDFAL 2865
Cdd:PRK12316 3230 QAYGLGVGDRVLQFTTFSFDVFVEELFWPLMSGARVVLAGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAHR 3309

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2866 PPGFK-ALCGGEALPENLATALLQKVtTLWNLYGPTETTIWSTLNGLTTP---TPYIGHPIANTQIYILDAQGRVVPLGV 2941
Cdd:PRK12316 3310 CTSLKrIVCGGEALPADLQQQVFAGL-PLYNLYGPTEATITVTHWQCVEEgkdAVPIGRPIANRACYILDGSLEPVPVGA 3388

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2942 AGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRL 3021
Cdd:PRK12316 3389 LGELYLGGEGLARGYHNRPGLTAERFVPDPF--VPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARL 3466

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3022 ARCHGVHDAVVIAREdspgDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPD 3101
Cdd:PRK12316 3467 LEHPWVREAVVLAVD----GRQLVAYVVPEDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPD 3542

                        2090      2100      2110      2120      2130      2140      2150      2160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3102 qSAMATRGYEAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPiVAIFQHPQLSALAE 3181
Cdd:PRK12316 3543 -AALLQQDYVAPVNELERRLAAIWADVLKLEQVGLTDNFFELGGDSIISLQVVSRARQAGIRFTP-KDLFQHQTIQGLAR 3620


                  .
gi 641744967 3182 V 3182
Cdd:PRK12316 3621 V 3621
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 1051-2406 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 1218.55  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1051 TAHDAPFTLPVADRTQPLPLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSID 1130
Cdd:COG1020     1 AAAAAAAALPPAAAAAPLPLSAAQQRLWLLLLLLLG-SAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRTRA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1131 GQPAQQIDPDtLGFSLSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWS 1210
Cdd:COG1020    80 GRPVQVIQPV-VAAPLPVVVLLVDLEALAEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLLLLLLLLLALHHIISDGLS 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1211 IGILARELAALYQAALEGSEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAG 1290
Cdd:COG1020   159 DGLLLAELLRLYLAAYAGAPLPLPPLPIQYADYALWQREWLQGEELARQLAYWRQQLAGLPPLLELPTDRPRPAVQSYRG 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1291 DQVPFHLDAGQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGR 1370
Cdd:COG1020   239 ARVSFRLPAELTAALRALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGRPRPELEGLVGFFVNTLPLRVDLSG 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1371 CHAVADLLDQVRERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQALTLPDLTLSAVERPQHSTHFDL 1450
Cdd:COG1020   319 DPSFAELLARVRETLLAAYAHQDLPFERLVEELQPERDLSRNPLFQVMFVLQNAPADELELPGLTLEPLELDSGTAKFDL 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1451 SLSLIETENGLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDALI 1530
Cdd:COG1020   399 TLTVVETGDGLRLTLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQLLAEWNATAAPYPADATL 478

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDP 1610
Cdd:COG1020   479 HELFEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDP 558

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1611 GYPAERLAYMLDDASPVALLTQANQRALLTG-DVPRILLDTADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMN 1689
Cdd:COG1020   559 AYPAERLAYMLEDAGARLVLTQSALAARLPElGVPVLALDALALAAEPATNPPVP-VTPDDLAYVIYTSGSTGRPKGVMV 637

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPS 1769
Cdd:COG1020   638 EHRALVNLLAWMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLTPS 717

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1770 MLQQFVQWADAdcACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDDHRS-FVPIGRPIA 1847
Cdd:COG1020   718 LLRALLDAAPE--ALPSLRLVLVGGEALPPELVRRWRARLpGARLVNLYGPTETTVDSTYYEVTPPDADGgSVPIGRPIA 795

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1848 NTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDF 1927
Cdd:COG1020   796 NTRVYVLDAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPF-GFPGARLYRTGDLARWLPDGNLEFLGRADD 874

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1928 QVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDA 2007
Cdd:COG1020   875 QVKIRGFRIELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLP 954

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2008 FPLTPNGKLDRKALPAPDQSAVATrdYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGLA 2087
Cdd:COG1020   955 LPLTGNGKLDRLALPAPAAAAAAA--AAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAARLLLL 1032

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2088 LDVRGVFSTPVLSDMAQAILAHQDKPAVVVPPNRIPADSTAITPDLLPLVTLTQpeidritdtvsggasniqdiypLAPL 2167
Cdd:COG1020  1033 LLLLLLLFLAAAAAAAAAAAAAAAAAAAAPLAAAAAPLPLPPLLLSLLALLLAL----------------------LLLL 1090

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2168 QEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILPINHFEPTSP 2247
Cdd:COG1020  1091 ALLALLALLLLLLLLLLLLALLLLLALLLALLAALRARRAVRQEGPRLRLLVALAAALALAALLALLLAAAAAAAELLAA 1170

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2248 EDVLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLPYR 2327
Cdd:COG1020  1171 AALLLLLALLLLALLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLAAAAAALLALALLLALLALAALLALA 1250

                        1290      1300      1310      1320      1330      1340      1350
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 2328 NFIAQTLSVPNSAHEAYFRDKLADVDEPTAPFGLLNVQGSGGDIHEARLVLDATLASAIRQQARHLGVSPGVLFHVAWA 2406
Cdd:COG1020  1251 ALAALAAALLALALALLALALLLLALALLLPALARARAARTARALALLLLLALLLLLALALALLLLLLLLLALLLLALL 1329
PRK12316 PRK12316
peptide synthase; Provisional
 1066-2459 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 1184.75  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1066 QPLPLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIdPDTLGFS 1145
Cdd:PRK12316   48 ERDRLSYAQQRMWFLWQLEPQ-SGAYNLPSAVRLNGPLDRQALERAFASLVQRHETLRTVFPRGADDSLAQV-PLDRPLE 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1146 LSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAA 1225
Cdd:PRK12316  126 VEFEDCSGLPEAEQEARLRDEAQRESLQPFDLCEGPLLRVRLLRLGEEEHVLLLTLHHIVSDGWSMNVLIEEFSRFYSAY 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1226 LEGSEANLPPLPVQYADYAVWQRQWLQ-GETLNDLrDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRR 1304
Cdd:PRK12316  206 ATGAEPGLPALPIQYADYALWQRSWLEaGEQERQL-EYWRAQLGEEHPVLELPTDHPRPAVPSYRGSRYEFSIDPALAEA 284

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1305 LHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRER 1384
Cdd:PRK12316  285 LRGTARRQGLTLFMLLLGAFNVLLHRYSGQTDIRVGVPIANRNRAEVEGLIGFFVNTQVLRSVFDGRTRVATLLAGVKDT 364

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1385 ALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQ---ALTLPDLTLSAVERPQHSTHFDLSLSLIETENGL 1461
Cdd:PRK12316  365 VLGAQAHQDLPFERLVEALKVERSLSHSPLFQVMYNHQPLVADieaLDTVAGLEFGQLEWKSRTTQFDLTLDTYEKGGRL 444

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1462 NGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPHDALIHQLVEDQAART 1541
Cdd:PRK12316  445 HAALTYATDLFEARTVERMARHWQNLLRGMVENPQARVDELPMLDAEERGQLVEGWNATAAEYPLQRGVHRLFEEQVERT 524

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:PRK12316  525 PEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYML 604

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLTQANQRALL--TGDVPRILLD--TADFSHLSEDNPHVpGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNR 1697
Cdd:PRK12316  605 EDSGVQLLLSQSHLGRKLplAAGVQVLDLDrpAAWLEGYSEENPGT-ELNPENLAYVIYTSGSTGKPKGAGNRHRALSNR 683

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1698 LVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQW 1777
Cdd:PRK12316  684 LCWMQQAYGLGVGDTVLQKTPFSFDVSVWEFFWPLMSGARLVVAAPGDHRDPAKLVELINREGVDTLHFVPSMLQAFLQD 763

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1778 ADADcACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDDHRSfVPIGRPIANTQLYILDT 1856
Cdd:PRK12316  764 EDVA-SCTSLRRIVCSGEALPADAQEQVFAKLpQAGLYNLYGPTEAAIDVTHWTCVEEGGDS-VPIGRPIANLACYILDA 841

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1857 LGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINqpGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRI 1936
Cdd:PRK12316  842 NLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVPSPFVA--GERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRI 919

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1937 ELGEIEARLMQCPGVQEAVVVAREDSpgdtRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:PRK12316  920 ELGEIEARLLEHPWVREAAVLAVDGK----QLVGYVVLESEGGDWREALKAHLAASLPEYMVPAQWLALERLPLTPNGKL 995

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2017 DRKALPAPDQSaVATRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGLALDVRGVFST 2096
Cdd:PRK12316  996 DRKALPAPEAS-VAQQGYVAPRNALERTLAAIWQDVLGVERVGLDDNFFELGGDSIVSIQVVSRARQAGIQLSPRDLFQH 1074

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2097 PVLSDMAQAILAHQdkpavvvppnripadstAITPDLLPlvtltqpeidritdtVSGGAsniqdiyPLAPLQEGILFHHL 2176
Cdd:PRK12316 1075 QTIRSLALVAKAGQ-----------------ATAADQGP---------------ASGEV-------ALAPVQRWFFEQAI 1115

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2177 LQEQ--GDTYLLRSmvafthRERLDAFL--SALQQVIDRHDILRTAVCWQDLSqpvqvvWRQAilpinhFEPTSPEDVLA 2252
Cdd:PRK12316 1116 PQRQhwNQSLLLQA------RQPLDPDRlgRALERLVAHHDALRLRFREEDGG------WQQA------YAAPQAGEVLW 1177

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2253 Q--------LQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLaLSFHHLISDHMTLALIVGEIRLLLQHQADALPTPL 2324
Cdd:PRK12316 1178 QrqaaseeeLLALCEEAQRSLDLEQGPLLRALLVDMADGSQRLL-LVIHHLVVDGVSWRILLEDLQRAYADLDADLPART 1256

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2325 PYRNFIAQTLS--VPNSAHE-AYFRDKLADvdeptAPFGLL--NVQGSGGDIHEARLV--LDATLASAIRQQArhlgvsP 2397
Cdd:PRK12316 1257 SSYQAWARRLHehAGARAEElDYWQAQLED-----APHELPceNPDGALENRHERKLElrLDAERTRQLLQEA------P 1325

                        1370      1380      1390      1400      1410      1420      1430
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967 2398 GV-------LFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGAD--RIMGMFINTLPLRISLADRGAAEV 2459
Cdd:PRK12316 1326 AAyrtqvndLLLTALARVTCRWSGQASVLVQLEGHGREDLFEDIDlsRTVGWFTSLFPVRLTPAADLGESI 1396
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 16-1328 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 940.05  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   16 ATVPDGVANVQDIYPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAIC--WQGLHQ 93
Cdd:COG1020     5 AAAALPPAAAAAPLPLSAAQQRLWLLLLLLLGSAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRtrAGRPVQ 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   94 PVQVVWRQAPLTVNTLTTTSSDTVPAQLRAATDPSNHRLNLSNAPLLSATTAHDPVcGEWLLSLSIHHLISDHITQALII 173
Cdd:COG1020    85 VIQPVVAAPLPVVVLLVDLEALAEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLL-LLLLLLLALHHIISDGLSDGLLL 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  174 DEIRLLLEDRPEALPKPLP---------YRNFIAQILSVPLSEHEQYFRNRLA-DIDTPTAPFDLVDVQGNGEDITEARL 243
Cdd:COG1020   164 AELLRLYLAAYAGAPLPLPplpiqyadyALWQREWLQGEELARQLAYWRQQLAgLPPLLELPTDRPRPAVQSYRGARVSF 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  244 SLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQgnLGADRVMGMFINTLPLRVSLR-ERS 322
Cdd:COG1020   244 RLPAELTAALRALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGRPR--PELEGLVGFFVNTLPLRVDLSgDPS 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  323 VHDVVQATSHELMMLLAHEQAPLALAQ---QCSQVPPPLPLFSTLFNYRHSQKDASSQFWEGMRQLS-GRERTNYPITLS 398
Cdd:COG1020   322 FAELLARVRETLLAAYAHQDLPFERLVeelQPERDLSRNPLFQVMFVLQNAPADELELPGLTLEPLElDSGTAKFDLTLT 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  399 VDDLGDGFNLTAKTVMGVDPERIVHYMLTAIENLVTSLEKTPQQPALSQPILPKSERQQVLVDFNATDADFPREMLIQQR 478
Cdd:COG1020   402 VVETGDGLRLTLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQLLAEWNATAAPYPADATLHEL 481

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  479 FEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:COG1020   482 FEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYP 561

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTQSAQVAQL-NSTLPTVLLDTPAAAACPDTNPVVqGLHAAHLAYVIYTSGSTGRPKGVMVAHR 637
Cdd:COG1020   562 AERLAYMLEDAGARLVLTQSALAARLpELGVPVLALDALALAAEPATNPPV-PVTPDDLAYVIYTSGSTGRPKGVMVEHR 640

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  638 NVINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQ 716
Cdd:COG1020   641 ALVNLLAWMQRRYGLGPGDRVLQFASLSFDASVWEiFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLTPSLLR 720

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  717 WLLDAGLGSDPayQPAQVLIGGEAISPAVWSRLQS-LSDTRFINVYGPTECTVDATACVV---DRTQPLPTIGKPLANTR 792
Cdd:COG1020   721 ALLDAAPEALP--SLRLVLVGGEALPPELVRRWRArLPGARLVNLYGPTETTVDSTYYEVtppDADGGSVPIGRPIANTR 798

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  793 LYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSAdPAARIYKTGDLARWLPDGNIDYLGRNDFQIK 872
Cdd:COG1020   799 VYVLDAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPFGF-PGARLYRTGDLARWLPDGNLEFLGRADDQVK 877

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  873 VRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPL 952
Cdd:COG1020   878 IRGFRIELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPL 957

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  953 TPNGKLDRKALPAPDQTafATRDYEAPQGGIETALAALWQELLGLDRVGRHDQFFALGGHSLLAVQLLNRMNKAGMDVAL 1032
Cdd:COG1020   958 TGNGKLDRLALPAPAAA--AAAAAAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAARLLLLLLL 1035

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1033 ATLFAHPTLCDLAAAVTATAHDAPFTLPVADRTQPLPLSFSQQRLWFLAQLDPAASQAYHLPAALHLTGRLDRPALTTAL 1112
Cdd:COG1020  1036 LLLLFLAAAAAAAAAAAAAAAAAAAAPLAAAAAPLPLPPLLLSLLALLLALLLLLALLALLALLLLLLLLLLLLALLLLL 1115

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1113 NGLVARHESLRTRFTSIDGQPAQQIDPDTLgfsLSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDD 1192
Cdd:COG1020  1116 ALLLALLAALRARRAVRQEGPRLRLLVALA---AALALAALLALLLAAAAAAAELLAAAALLLLLALLLLALLLLLLLLL 1192

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1193 NTHVLLLTQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPA 1272
Cdd:COG1020  1193 LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLAAAAAALLALALLLALLALAALLALAALAALAAALLALALALLALALL 1272

                        1290      1300      1310      1320      1330
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 1273 LLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHALNRQQGTTLFMTLLAAWSVVL 1328
Cdd:COG1020  1273 LLALALLLPALARARAARTARALALLLLLALLLLLALALALLLLLLLLLALLLLAL 1328
PRK12316 PRK12316
peptide synthase; Provisional
 1059-2111 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 882.37  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1059 LPVADRTQPLPLSFSQQRLWFLAQLDPAASQaYHLPAALHLTGrLDRPALTTALNGLVARHESLRTRFTSID--GQPAQQ 1136
Cdd:PRK12316 4094 LPLGEIEDIYPLSPMQQGMLFHSLYEQEAGD-YINQMRVDVQG-LDVERFRAAWQAALDRHDVLRSGFVWQGelGRPLQV 4171

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1137 IDpDTLGFSLSSHDLRKldEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILAR 1216
Cdd:PRK12316 4172 VH-KQVSLPFAELDWRG--RADLQAALDALAAAERERGFDLQRAPLLRLVLVRTAEGRHHLIYTNHHILMDGWSNSQLLG 4248

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1217 ELAALYqaalegSEANLPPLPVQYADYAVWqrqwLQGETLNDLRDYWRDQLqgapALLEIPTdRPRPSVQRYAGDQVPFH 1296
Cdd:PRK12316 4249 EVLERY------SGRPPAQPGGRYRDYIAW----LQRQDAAASEAFWREQL----AALDEPT-RLAQAIARADLRSANGY 4313

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1297 ------LDAGQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRP--RQELEGMVGFFVNTLALRTEP 1368
Cdd:PRK12316 4314 gehvreLDATATARLREFARTQRVTLNTLVQAAWLLLLQRYTGQDTVAFGATVAGRPaeLPGIEGQIGLFINTLPVIATP 4393

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1369 GRCHAVADLLDQVRERALDAYAHQALPFEQvveiLQPARSLSYSPIFQVMLSLNNTPA----QALTLPDLTLSAVE-RPQ 1443
Cdd:PRK12316 4394 RAQQSVVEWLQQVQRQNLALREHEHTPLYE----IQRWAGQGGEALFDSLLVFENYPVsealQQGAPGGLRFGEVTnHEQ 4469

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1444 HSTHFDLSLSLIETengLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEAD 1523
Cdd:PRK12316 4470 TNYPLTLAVGLGET---LSLQFSYDRGHFDAATIERLARHLTNLLEAMAEDPQRRLGELQLLEKAEQQRIVALWNRTDAG 4546

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1524 FPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGA 1603
Cdd:PRK12316 4547 YPATRCVHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGG 4626

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1604 AYVPLDPGYPAERLAYMLDDASPVALLTQAN--QRALLTGDVPRILLD-TADFSHLSEDNPHVPgLDAHHLAYVIYTSGS 1680
Cdd:PRK12316 4627 AYVPLDPEYPRERLAYMMEDSGAALLLTQSHllQRLPIPDGLASLALDrDEDWEGFPAHDPAVR-LHPDNLAYVIYTSGS 4705

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1681 TGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMaRPDGHKDAAYLAQLIERTG 1760
Cdd:PRK12316 4706 TGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSFDGSHEGLYHPLINGASVVI-RDDSLWDPERLYAEIHEHR 4784

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1761 ITTLHFVPSMLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDDH--R 1837
Cdd:PRK12316 4785 VTVLVFPPVYLQQLAEHAERDGEPPSLRVYCFGGEAVAQASYDLAWRALkPVYLFNGYGPTETTVTVLLWKARDGDAcgA 4864

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1838 SFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDG 1917
Cdd:PRK12316 4865 AYMPIGTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVARGYLERPALTAERFVPDPF-GAPGGRLYRTGDLARYRADG 4943

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1918 SLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGdTRLVAYLCPQ-PGVTPDP---ADLRQQLGQHL 1993
Cdd:PRK12316 4944 VIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAVG-KQLVGYVVPQdPALADADeaqAELRDELKAAL 5022

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1994 A----EYMVPGAFVTLDAFPLTPNGKLDRKALPAPDQSAvATRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGG 2069
Cdd:PRK12316 5023 RerlpEYMVPAHLVFLARMPLTPNGKLDRKALPQPDASL-LQQAYVAPRSELEQQVAAIWAEVLQLERVGLDDNFFELGG 5101

                        1050      1060      1070      1080
                  ....*....|....*....|....*....|....*....|...
gi 641744967 2070 HSLLIVSLIERLRRA-GLALDVRGVFSTPVLSDMAQAiLAHQD 2111
Cdd:PRK12316 5102 HSLLAIQVTSRIQLElGLELPLRELFQTPTLAAFVEL-AAAAG 5143
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 2159-3191 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 868.40  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2159 QDIYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVC--WQDLSQPVQVVWRQAI 2236
Cdd:COG1020    15 AAPLPLSAAQQRLWLLLLLLLGSAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRtrAGRPVQVIQPVVAAPL 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2237 LPINHFEPTSPEDVLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQnEWLLALSFHHLISDHMTLALIVGEIRLLLQHQ 2316
Cdd:COG1020    95 PVVVLLVDLEALAEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLLL-LLLLLLALHHIISDGLSDGLLLAELLRLYLAA 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2317 ADALPTPLP---------YRNFIAQTLSVPNSAHEAYFRDKLA-DVDEPTAPFGLLNVQGSGGDIHEARLVLDATLASAI 2386
Cdd:COG1020   174 YAGAPLPLPplpiqyadyALWQREWLQGEELARQLAYWRQQLAgLPPLLELPTDRPRPAVQSYRGARVSFRLPAELTAAL 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2387 RQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISL-ADRGAAEVVERTSH 2465
Cdd:COG1020   254 RALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGR--PRPELEGLVGFFVNTLPLRVDLsGDPSFAELLARVRE 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2466 DLMTLLEHEQAPLALAQR---CSGVAPPMPLFSTLLNYRHTQASSTDNTLSDIRVLT-SEERTNYPLTLAVDDRGEGFSL 2541
Cdd:COG1020   332 TLLAAYAHQDLPFERLVEelqPERDLSRNPLFQVMFVLQNAPADELELPGLTLEPLElDSGTAKFDLTLTVVETGDGLRL 411

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2542 VAQTLEDIDPHRLLNYLMTAISSLVDALETEPQRSILNLPVLPDSERQQMLVDFNATDADIPRHALIHELFEAQVACTPD 2621
Cdd:COG1020   412 TLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQLLAEWNATAAPYPADATLHELFEAQAARTPD 491

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2622 AIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDD 2701
Cdd:COG1020   492 AVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAERLAYMLED 571

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2702 AKPVALISQSAHLG-IMNGSLPVILLDDGEtrpFDNEPDTPLDArkqGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNF 2780
Cdd:COG1020   572 AGARLVLTQSALAArLPELGVPVLALDALA---LAAEPATNPPV---PVTPDDLAYVIYTSGSTGRPKGVMVEHRALVNL 645

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2781 LCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVEL 2860
Cdd:COG1020   646 LAWMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLTPSLLRALLDA 725

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2861 RDFALPPGFKALCGGEALPENLATALLQKV--TTLWNLYGPTETTIWSTLNGLTTPTPY-----IGHPIANTQIYILDAQ 2933
Cdd:COG1020   726 APEALPSLRLVLVGGEALPPELVRRWRARLpgARLVNLYGPTETTVDSTYYEVTPPDADggsvpIGRPIANTRVYVLDAH 805

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2934 GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIE 3013
Cdd:COG1020   806 LQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPF-GFPGARLYRTGDLARWLPDGNLEFLGRADDQVKIRGFRIE 884

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3014 LGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLD 3093
Cdd:COG1020   885 LGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLD 964

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3094 RKALPAPDQSAMATrgYEAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQH 3173
Cdd:COG1020   965 RLALPAPAAAAAAA--AAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAARLLLLLLLLLLLFLA 1042

                        1050
                  ....*....|....*...
gi 641744967 3174 PQLSALAEVILAAQIHAA 3191
Cdd:COG1020  1043 AAAAAAAAAAAAAAAAAA 1060
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
1061-2111 0e+00

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 865.51  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1061 VADRTQPLPLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPD 1140
Cdd:PRK10252    1 AEPMSQHLPLVAAQPGIWMAEKLSPLPS-AWSVAHYVELTGELDAPLLARAVVAGLAEADTLRMRFTEDNGEVWQWVDPA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1141 TLGFSLSSHDLRKLDEAARTTRVaeLAEQEARARFDLTQG-PLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELA 1219
Cdd:PRK10252   80 LTFPLPEIIDLRTQPDPHAAAQA--LMQADLQQDLRVDSGkPLVFHQLIQLGDNRWYWYQRYHHLLVDGFSFPAITRRIA 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1220 ALYQAALEGSEANLPPLPVQ---YADYAVWQrqwlQGETLNDLRDYWRDQLQGAPallEIPTDRPRPSVQRYAGD---QV 1293
Cdd:PRK10252  158 AIYCAWLRGEPTPASPFTPFadvVEEYQRYR----ASEAWQRDAAFWAEQRRQLP---PPASLSPAPLPGRSASAdilRL 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1294 PFHLDAGQLRRLHALNRQ-QGTTLFMTLLAAWsvvLSRLSGQDDIVIGTPVANR---PRQELEGMVgffVNTLALRTEPG 1369
Cdd:PRK10252  231 KLEFTDGAFRQLAAQASGvQRPDLALALVALW---LGRLCGRMDYAAGFIFMRRlgsAALTATGPV---LNVLPLRVHIA 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1370 RCHAVADLLDQVRERALDAYAHQALPFEQVVEILQPA---RSLsYSPIFQVM-----LSLNNTPAQALTLpdlTLSAVEr 1441
Cdd:PRK10252  305 AQETLPELATRLAAQLKKMRRHQRYDAEQIVRDSGRAagdEPL-FGPVLNIKvfdyqLDFPGVQAQTHTL---ATGPVN- 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1442 pqhsthfDLSLSL-IETENGLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMlDFNAT 1520
Cdd:PRK10252  380 -------DLELALfPDEHGGLSIEILANPQRYDEATLIAHAERLKALIAQFAADPALLCGDVDILLPGEYAQLA-QVNAT 451

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1521 EADFPHDALIhQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILK 1600
Cdd:PRK10252  452 AVEIPETTLS-ALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVE 530

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1601 AGAAYVPLDPGYPAERLAYMLDDASPVALLTQANQRALLTgDVPRILLDTADFSHLSEDNPHVPGLDAHHLAYVIYTSGS 1680
Cdd:PRK10252  531 AGAAWLPLDTGYPDDRLKMMLEDARPSLLITTADQLPRFA-DVPDLTSLCYNAPLAPQGAAPLQLSQPHHTAYIIFTSGS 609

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1681 TGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTG 1760
Cdd:PRK10252  610 TGRPKGVMVGQTAIVNRLLWMQNHYPLTADDVVLQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFFAEYG 689

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1761 ITTLHFVPSMLQQFVQWAD---ADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHR 1837
Cdd:PRK10252  690 VTTTHFVPSMLAAFVASLTpegARQSCASLRQVFCSGEALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWYPAFGEELA 769

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1838 SF----VPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFInqPGARLYKTGDLARW 1913
Cdd:PRK10252  770 AVrgssVPIGYPVWNTGLRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFA--PGERMYRTGDVARW 847

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1914 LPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAR-----EDSPGD-TRLVAYLCPQPGVTPDPADLRQ 1987
Cdd:PRK10252  848 LDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHACvinqaAATGGDaRQLVGYLVSQSGLPLDTSALQA 927

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1988 QLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAPD-QSAVATRdyeAPQGEVETALAAVWQDLLGLTRVGRHDHFFA 2066
Cdd:PRK10252  928 QLRERLPPHMVPVVLLQLDQLPLSANGKLDRKALPLPElKAQVPGR---APKTGTETIIAAAFSSLLGCDVVDADADFFA 1004

                        1050      1060      1070      1080
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 2067 LGGHSLLIVSLIERLRRA-GLALDVRGVFSTPVLSDMAQAILAHQD 2111
Cdd:PRK10252 1005 LGGHSLLAMKLAAQLSRQfARQVTPGQVMVASTVAKLATLLDAEED 1050
PRK12467 PRK12467
peptide synthase; Provisional
 1059-2129 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 848.29  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1059 LPVADRTQPLPLSFSQQRLWFLAQLDPAASQaYHLPAALHLTGrLDRPALTTALNGLVARHESLRTRFTSIDG--QPAQQ 1136
Cdd:PRK12467 2638 VAVGDIEDIYPLSPMQQGMLFHTLYEGGAGD-YINQMRVDVEG-LDVERFRTAWQAVIDRHEILRSGFLWDGEleEPLQV 2715

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1137 IDPD-TLGFSlsSHDLRklDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILA 1215
Cdd:PRK12467 2716 VYKQaRLPFS--RLDWR--DRADLEQALDALAAADRQQGFDLLSAPLLRLTLVRTGEDRHHLIYTNHHILMDGWSGSQLL 2791

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1216 RELAALYqaalegSEANLPPLPVQYADYAvwqrQWLQGETLNDLRDYWRDQLQ--GAPALLeIPTDRPRPSVQRYAGDQV 1293
Cdd:PRK12467 2792 GEVLQRY------FGQPPPAREGRYRDYI----AWLQAQDAEASEAFWKEQLAalEEPTRL-ARALYPAPAEAVAGHGAH 2860

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1294 PFHLDAGQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQ--ELEGMVGFFVNTLALRTEPGRC 1371
Cdd:PRK12467 2861 YLHLDATQTRQLIEFARRHRVTLNTLVQGAWLLLLQRFTGQDTVCFGATVAGRPAQlrGAEQQLGLFINTLPVIASPRAE 2940

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1372 HAVADLLDQVRERALDAYAHQALPFEQVveilQPARSLSYSPIFQVMLSLNNTP-AQAL---TLPDLTLSAVE-RPQHST 1446
Cdd:PRK12467 2941 QTVSDWLQQVQAQNLALREFEHTPLADI----QRWAGQGGEALFDSILVFENYPiSEALkqgAPSGLRFGAVSsREQTNY 3016

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1447 HFDLSLSLIETengLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFPH 1526
Cdd:PRK12467 3017 PLTLAVGLGDT---LELEFSYDRQHFDAAAIERLAESFDRLLQAMLNNPAARLGELPTLAAHERRQVLHAWNATAAAYPS 3093

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1527 DALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYV 1606
Cdd:PRK12467 3094 ERLVHQLIEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYV 3173

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1607 PLDPGYPAERLAYMLDDASPVALLTQAN---QRALLTGDVpRILLDTADFSHLSEDNPhVPGLDAHHLAYVIYTSGSTGK 1683
Cdd:PRK12467 3174 PLDPEYPRERLAYMIEDSGVKLLLTQAHlleQLPAPAGDT-ALTLDRLDLNGYSENNP-STRVMGENLAYVIYTSGSTGK 3251

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1684 PKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMaRPDGHKDAAYLAQLIERTGITT 1763
Cdd:PRK12467 3252 PKGVGVRHGALANHLCWIAEAYELDANDRVLLFMSFSFDGAQERFLWTLICGGCLVV-RDNDLWDPEELWQAIHAHRISI 3330

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1764 LHFVPSMLQQFVQWAD-ADCAcdSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPD--DHRSF 1839
Cdd:PRK12467 3331 ACFPPAYLQQFAEDAGgADCA--SLDIYVFGGEAVPPAAFEQVKRKLkPRGLTNGYGPTEAVVTVTLWKCGGDavCEAPY 3408

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1840 VPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINQpGARLYKTGDLARWLPDGSL 1919
Cdd:PRK12467 3409 APIGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADPFSGS-GGRLYRTGDLARYRADGVI 3487

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1920 EYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAReDSPGDTRLVAYLCPQpgvtpDP-ADLRQQLGQHLA---- 1994
Cdd:PRK12467 3488 EYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLAR-DGAGGKQLVAYVVPA-----DPqGDWRETLRDHLAaslp 3561

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1995 EYMVPGAFVTLDAFPLTPNGKLDRKALPAPDqsAVATRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLI 2074
Cdd:PRK12467 3562 DYMVPAQLLVLAAMPLGPNGKVDRKALPDPD--AKGSREYVAPRSEVEQQLAAIWADVLGVEQVGVTDNFFELGGDSLLA 3639

                        1050      1060      1070      1080      1090
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 2075 VSLIERLRRA-GLALDVRGVFSTPVLSDMAQAILAHQDKPAVVVPPNRIPADSTAI 2129
Cdd:PRK12467 3640 LQVLSRIRQSlGLKLSLRDLMSAPTIAELAGYSPLGDVPVNLLLDLNRLETGFPAL 3695
PRK12316 PRK12316
peptide synthase; Provisional
 2129-3186 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 803.41  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2129 ITPDLLPLVTLTQPEIDritdTVSGGASNIQDIYPLAPLQEGILFHHLLQEQGDTYL--LRSMVAFTHRERldaFLSALQ 2206
Cdd:PRK12316 4074 VTPSDFPLAGLDQARLD----ALPLPLGEIEDIYPLSPMQQGMLFHSLYEQEAGDYInqMRVDVQGLDVER---FRAAWQ 4146

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2207 QVIDRHDILRTAVCWQD-LSQPVQVVWRQAILPINHFEPTSPEDVLAQLQA-HTEPRTRRIDLSQAPLFRADIAHDPlQN 2284
Cdd:PRK12316 4147 AALDRHDVLRSGFVWQGeLGRPLQVVHKQVSLPFAELDWRGRADLQAALDAlAAAERERGFDLQRAPLLRLVLVRTA-EG 4225

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2285 EWLLALSFHHLISDHMTLALIVGEIrllLQHQADALPTP--LPYRNFIAQTLSVPNSAHEAYFRDKLADVDEPT------ 2356
Cdd:PRK12316 4226 RHHLIYTNHHILMDGWSNSQLLGEV---LERYSGRPPAQpgGRYRDYIAWLQRQDAAASEAFWREQLAALDEPTrlaqai 4302

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2357 APFGLLNVQGSGGDIHEarlvLDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADR 2436
Cdd:PRK12316 4303 ARADLRSANGYGEHVRE----LDATATARLREFARTQRVTLNTLVQAAWLLLLQRYTGQDTVAFGATVAGRPAELPGIEG 4378

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2437 IMGMFINTLPLRISL-ADRGAAEVVERTSHDLMTLLEHEQAPLALAQRCSGVAPPmPLFSTLLNYRHTQASST--DNTLS 2513
Cdd:PRK12316 4379 QIGLFINTLPVIATPrAQQSVVEWLQQVQRQNLALREHEHTPLYEIQRWAGQGGE-ALFDSLLVFENYPVSEAlqQGAPG 4457

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2514 DIRV--LTSEERTNYPLTLAVDdRGEGFSL---------VAQTLEDIDPHrllnylmtaISSLVDALETEPQRSILNLPV 2582
Cdd:PRK12316 4458 GLRFgeVTNHEQTNYPLTLAVG-LGETLSLqfsydrghfDAATIERLARH---------LTNLLEAMAEDPQRRLGELQL 4527

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2583 LPDSERQQMLVDFNATDADIPRHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAIC 2662
Cdd:PRK12316 4528 LEKAEQQRIVALWNRTDAGYPATRCVHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIA 4607

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2663 VERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQS---AHLGIMNG--SLPVILLDDGETRPfDNE 2737
Cdd:PRK12316 4608 MERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTQShllQRLPIPDGlaSLALDRDEDWEGFP-AHD 4686

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2738 PDTPLDarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLN 2817
Cdd:PRK12316 4687 PAVRLH-------PDNLAYVIYTSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSFDGSHEGLYHPLIN 4759

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2818 GARLILATQAQAADAQQLAmLIERHAVSFMQATPSTWRMLVE--LRDFALPPGFKALCGGEALPENLATALLQ--KVTTL 2893
Cdd:PRK12316 4760 GASVVIRDDSLWDPERLYA-EIHEHRVTVLVFPPVYLQQLAEhaERDGEPPSLRVYCFGGEAVAQASYDLAWRalKPVYL 4838

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2894 WNLYGPTETTIWSTLNGLTTPTP------YIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERF 2967
Cdd:PRK12316 4839 FNGYGPTETTVTVLLWKARDGDAcgaaymPIGTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVARGYLERPALTAERF 4918

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2968 ITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGdKRLVAY 3047
Cdd:PRK12316 4919 VPDPF-GAPGGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAVG-KQLVGY 4996

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3048 LLAQpDTVLEPAD---------LRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPDQSAmATRGYEAPQGDLE 3118
Cdd:PRK12316 4997 VVPQ-DPALADADeaqaelrdeLKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKALPQPDASL-LQQAYVAPRSELE 5074

                        1050      1060      1070      1080      1090      1100
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 3119 HALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILAA 3186
Cdd:PRK12316 5075 QQVAAIWAEVLQLERVGLDDNFFELGGHSLLAIQVTSRIQLELGLELPLRELFQTPTLAAFVELAAAA 5142
PRK12467 PRK12467
peptide synthase; Provisional
 2-1252 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 772.41  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    2 PLVTLSQEEIDTViatvPDGVANVQDIYPLAPLQEGILFHYQLQEKGDTYLlnsllafdSQTRLD-------AFLDVLQQ 74
Cdd:PRK12467 2624 PLAGLSQEQLDRL----PVAVGDIEDIYPLSPMQQGMLFHTLYEGGAGDYI--------NQMRVDvegldveRFRTAWQA 2691

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   75 VIARHDILRTAICWQG-LHQPVQVVWRQAPLTVNTLTTTSSDTVPAQLR--AATDPSNHrLNLSNAPLLSAT---TAHDp 148
Cdd:PRK12467 2692 VIDRHEILRSGFLWDGeLEEPLQVVYKQARLPFSRLDWRDRADLEQALDalAAADRQQG-FDLLSAPLLRLTlvrTGED- 2769

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  149 vcgEWLLSLSIHHLISDHITQALIIDEIrlLLEDRPEALPKP-LPYRNFIAQILSVPLSEHEQYFRNRLADIDTPTA--- 224
Cdd:PRK12467 2770 ---RHHLIYTNHHILMDGWSGSQLLGEV--LQRYFGQPPPAReGRYRDYIAWLQAQDAEASEAFWKEQLAALEEPTRlar 2844

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  225 --PFDLVD-VQGNGEditeARLSLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGA 301
Cdd:PRK12467 2845 alYPAPAEaVAGHGA----HYLHLDATQTRQLIEFARRHRVTLNTLVQGAWLLLLQRFTGQDTVCFGATVAGRPAQLRGA 2920

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  302 DRVMGMFINTLPLRVSLR-ERSVHDVVQATSHELMMLLAHEQAPLALAQQCSQVPPPlPLFSTLF---NYRHSQ--KDAS 375
Cdd:PRK12467 2921 EQQLGLFINTLPVIASPRaEQTVSDWLQQVQAQNLALREFEHTPLADIQRWAGQGGE-ALFDSILvfeNYPISEalKQGA 2999

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  376 SQfweGMR--QLSGRERTNYPITLSV---DDLGDGFNLTAKTVMGVDPERIVHYMltaiENLVTSLEKTPQQPALSQPIL 450
Cdd:PRK12467 3000 PS---GLRfgAVSSREQTNYPLTLAVglgDTLELEFSYDRQHFDAAAIERLAESF----DRLLQAMLNNPAARLGELPTL 3072

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  451 PKSERQQVLVDFNATDADFPREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCV 530
Cdd:PRK12467 3073 AAHERRQVLHAWNATAAAYPSERLVHQLIEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAV 3152

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  531 ERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLN--STLPTVLLDTPAAAACPDTNPVV 608
Cdd:PRK12467 3153 ERSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLLTQAHLLEQLPapAGDTALTLDRLDLNGYSENNPST 3232

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  609 QgLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWI-QLLSGHTLVLVPD 687
Cdd:PRK12467 3233 R-VMGENLAYVIYTSGSTGKPKGVGVRHGALANHLCWIAEAYELDANDRVLLFMSFSFDGAQERFLwTLICGGCLVVRDN 3311

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  688 ALRaDAHQLWRYFARHAVDLFDCTPVQLQWLL-DAGLGSDPAYQpaQVLIGGEAISPAVWSRLQS-LSDTRFINVYGPTE 765
Cdd:PRK12467 3312 DLW-DPEELWQAIHAHRISIACFPPAYLQQFAeDAGGADCASLD--IYVFGGEAVPPAAFEQVKRkLKPRGLTNGYGPTE 3388

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  766 CTVDAT--ACVVDRTQPLPT--IGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSaD 841
Cdd:PRK12467 3389 AVVTVTlwKCGGDAVCEAPYapIGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADPFS-G 3467

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  842 PAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAReDSPGDTRLVAYLCARPDAE 921
Cdd:PRK12467 3468 SGGRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLAR-DGAGGKQLVAYVVPADPQG 3546

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  922 LHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDqtAFATRDYEAPQGGIETALAALWQELLGLDRVG 1001
Cdd:PRK12467 3547 DWRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDPD--AKGSREYVAPRSEVEQQLAAIWADVLGVEQVG 3624

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1002 RHDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAVtatahdapftlpvadrtqplplsfsqqrlwfl 1080
Cdd:PRK12467 3625 VTDNFFELGGDSLLALQVLSRIRQSlGLKLSLRDLMSAPTIAELAGYS-------------------------------- 3672

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1081 aqldpaasQAYHLPAALhltgRLDRPALTTALNGLVARHESLRTRFtsiDGQPAQQIdpdtlgfslsshdlrkldeaart 1160
Cdd:PRK12467 3673 --------PLGDVPVNL----LLDLNRLETGFPALFCRHEGLGTVF---DYEPLAVI----------------------- 3714

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1161 trvaelaeqeararfdltqgplirgqllqLDDNTHVLLLTQHHIISDGWsigilarelaalyqaalegSEANLPPLPVQY 1240
Cdd:PRK12467 3715 -----------------------------LEGDRHVLGLTCRHLLDDGW-------------------QDTSLQAMAVQY 3746

                        1290
                  ....*....|..
gi 641744967 1241 ADYAVWQRQWLQ 1252
Cdd:PRK12467 3747 ADYILWQQAKGP 3758
PRK12467 PRK12467
peptide synthase; Provisional
 2124-3203 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 764.70  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2124 ADSTAITPDLLPLVTLTQPEIDRITDTVSggasNIQDIYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFthrERLDA--F 2201
Cdd:PRK12467 2613 NDQRGVTPSDFPLAGLSQEQLDRLPVAVG----DIEDIYPLSPMQQGMLFHTLYEGGAGDYINQMRVDV---EGLDVerF 2685

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2202 LSALQQVIDRHDILRTAVCWQD-LSQPVQVVWRQAILPINHFEPTSPEDVLAQLQAHTEP-RTRRIDLSQAPLFRADIAH 2279
Cdd:PRK12467 2686 RTAWQAVIDRHEILRSGFLWDGeLEEPLQVVYKQARLPFSRLDWRDRADLEQALDALAAAdRQQGFDLLSAPLLRLTLVR 2765

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2280 DPlQNEWLLALSFHHLISDHMTLALIVGEIrllLQHQA-DALPTP-LPYRNFIAQTLSVPNSAHEAYFRDKLADVDEPT- 2356
Cdd:PRK12467 2766 TG-EDRHHLIYTNHHILMDGWSGSQLLGEV---LQRYFgQPPPAReGRYRDYIAWLQAQDAEASEAFWKEQLAALEEPTr 2841

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2357 --APFGLLNVQGSGGdiHEAR-LVLDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEG 2433
Cdd:PRK12467 2842 laRALYPAPAEAVAG--HGAHyLHLDATQTRQLIEFARRHRVTLNTLVQGAWLLLLQRFTGQDTVCFGATVAGRPAQLRG 2919

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2434 ADRIMGMFINTLPLRISL-ADRGAAEVVERTSHDLMTLLEHEQAPLALAQRCSGVaPPMPLFSTLL---NYRHTQASStD 2509
Cdd:PRK12467 2920 AEQQLGLFINTLPVIASPrAEQTVSDWLQQVQAQNLALREFEHTPLADIQRWAGQ-GGEALFDSILvfeNYPISEALK-Q 2997

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2510 NTLSDIRV--LTSEERTNYPLTLAV---DDRGEGFSLVAQTLEDIDPHRLLNYLMTAISSLVDAletePQRSILNLPVLP 2584
Cdd:PRK12467 2998 GAPSGLRFgaVSSREQTNYPLTLAVglgDTLELEFSYDRQHFDAAAIERLAESFDRLLQAMLNN----PAARLGELPTLA 3073

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2585 DSERQQMLVDFNATDADIPRHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVE 2664
Cdd:PRK12467 3074 AHERRQVLHAWNATAAAYPSERLVHQLIEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVE 3153

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2665 RGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQSA---HLGIMNGSLpVILLDDGETRPF-DNEPDT 2740
Cdd:PRK12467 3154 RSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLLTQAHlleQLPAPAGDT-ALTLDRLDLNGYsENNPST 3232

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2741 PLDarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGAR 2820
Cdd:PRK12467 3233 RVM-------GENLAYVIYTSGSTGKPKGVGVRHGALANHLCWIAEAYELDANDRVLLFMSFSFDGAQERFLWTLICGGC 3305

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2821 LILATQAQAADAQQLAmLIERHAVSFMQATPSTWRMLVELRDFA-LPPGFKALCGGEALPENLATALLQKV--TTLWNLY 2897
Cdd:PRK12467 3306 LVVRDNDLWDPEELWQ-AIHAHRISIACFPPAYLQQFAEDAGGAdCASLDIYVFGGEAVPPAAFEQVKRKLkpRGLTNGY 3384

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2898 GPTETTI----WSTLNGLTTPTPY--IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDP 2971
Cdd:PRK12467 3385 GPTEAVVtvtlWKCGGDAVCEAPYapIGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADP 3464

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2972 FSGAPEaRMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAReDSPGDKRLVAYLLAQ 3051
Cdd:PRK12467 3465 FSGSGG-RLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLAR-DGAGGKQLVAYVVPA 3542

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3052 pdtvLEPADLRQRLSEGVA----EYMIPSAFVTLDAFPLTPNGKLDRKALPAPDqsAMATRGYEAPQGDLEHALAQIWQT 3127
Cdd:PRK12467 3543 ----DPQGDWRETLRDHLAaslpDYMVPAQLLVLAAMPLGPNGKVDRKALPDPD--AKGSREYVAPRSEVEQQLAAIWAD 3616

                        1050      1060      1070      1080      1090      1100      1110
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 3128 LLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILAAQIHAAWGNDTDALTHDL 3203
Cdd:PRK12467 3617 VLGVEQVGVTDNFFELGGDSLLALQVLSRIRQSLGLKLSLRDLMSAPTIAELAGYSPLGDVPVNLLLDLNRLETGF 3692
PRK12316 PRK12316
peptide synthase; Provisional
 3-1339 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 758.72  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    3 LVTLSQEEIDTVIATVPDGVAnVQDIYPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDIL 82
Cdd:PRK12316   25 LATLRGEGVDFSLFPIPAGVS-SAERDRLSYAQQRMWFLWQLEPQSGAYNLPSAVRLNGPLDRQALERAFASLVQRHETL 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   83 RTAICwQGLHQPVQVVWRQAPLTVNTLTTTSSDTVPAQLR---AATDPSNHRLNLSNAPLL-------SATtahdpvcgE 152
Cdd:PRK12316  104 RTVFP-RGADDSLAQVPLDRPLEVEFEDCSGLPEAEQEARlrdEAQRESLQPFDLCEGPLLrvrllrlGEE--------E 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  153 WLLSLSIHHLISDHITQALIIDE-IRL---LLEDR-PEALPKPLPYRNF-IAQILSVPLSEHE---QYFRNRLAD----I 219
Cdd:PRK12316  175 HVLLLTLHHIVSDGWSMNVLIEEfSRFysaYATGAePGLPALPIQYADYaLWQRSWLEAGEQErqlEYWRAQLGEehpvL 254

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  220 DTPT-APFDLV-DVQGngediTEARLSLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQG 297
Cdd:PRK12316  255 ELPTdHPRPAVpSYRG-----SRYEFSIDPALAEALRGTARRQGLTLFMLLLGAFNVLLHRYSGQTDIRVGVPIANRNRA 329

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  298 NLgaDRVMGMFINTLPLRVSLRER-SVHDVVQATSHELMMLLAHEQAPLALAQQCSQVPPPL---PLFSTLFNYRHSQKD 373
Cdd:PRK12316  330 EV--EGLIGFFVNTQVLRSVFDGRtRVATLLAGVKDTVLGAQAHQDLPFERLVEALKVERSLshsPLFQVMYNHQPLVAD 407

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  374 AssqfwEGMRQLSGRE---------RTNYPITLSVDDLGDGFNLTAKTVMGVDPERIVHYMLTAIENLVTSLEKTPQQPA 444
Cdd:PRK12316  408 I-----EALDTVAGLEfgqlewksrTTQFDLTLDTYEKGGRLHAALTYATDLFEARTVERMARHWQNLLRGMVENPQARV 482

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  445 LSQPILPKSERQQVLVDFNATDADFPREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDD 524
Cdd:PRK12316  483 DELPMLDAEERGQLVEGWNATAAEYPLQRGVHRLFEEQVERTPEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDV 562

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  525 RVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQL--NSTLPTVLLDTPAA--AA 600
Cdd:PRK12316  563 LVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLSQSHLGRKLplAAGVQVLDLDRPAAwlEG 642

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  601 CPDTNPVVQgLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASV-KNWIQLLSG 679
Cdd:PRK12316  643 YSEENPGTE-LNPENLAYVIYTSGSTGKPKGAGNRHRALSNRLCWMQQAYGLGVGDTVLQKTPFSFDVSVwEFFWPLMSG 721

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  680 HTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAglGSDPAYQPAQVLI-GGEAISPAVWSRL-QSLSDTRF 757
Cdd:PRK12316  722 ARLVVAAPGDHRDPAKLVELINREGVDTLHFVPSMLQAFLQD--EDVASCTSLRRIVcSGEALPADAQEQVfAKLPQAGL 799

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  758 INVYGPTECTVDAT--ACVVDRTQPLPtIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIP 835
Cdd:PRK12316  800 YNLYGPTEAAIDVThwTCVEEGGDSVP-IGRPIANLACYILDANLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVP 878

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  836 DPFSAdpAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSpgdtRLVAYLC 915
Cdd:PRK12316  879 SPFVA--GERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVDGK----QLVGYVV 952

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  916 ARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAfATRDYEAPQGGIETALAALWQELL 995
Cdd:PRK12316  953 LESEGGDWREALKAHLAASLPEYMVPAQWLALERLPLTPNGKLDRKALPAPEASV-AQQGYVAPRNALERTLAAIWQDVL 1031

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  996 GLDRVGRHDQFFALGGHSLLAVQLLNRMNKAGMDVALATLFAHPTLCDLAAAVTATAHDAPFTLPVADRTQPLPLsfsqQ 1075
Cdd:PRK12316 1032 GVERVGLDDNFFELGGDSIVSIQVVSRARQAGIQLSPRDLFQHQTIRSLALVAKAGQATAADQGPASGEVALAPV----Q 1107

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1076 RlWFLAQLDPaasQAYHLPAALHLTGR--LDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLGFSLSSHDLrk 1153
Cdd:PRK12316 1108 R-WFFEQAIP---QRQHWNQSLLLQARqpLDPDRLGRALERLVAHHDALRLRFREEDGGWQQAYAAPQAGEVLWQRQA-- 1181

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1154 LDEAArttrVAELAEqEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALegseANL 1233
Cdd:PRK12316 1182 ASEEE----LLALCE-EAQRSLDLEQGPLLRALLVDMADGSQRLLLVIHHLVVDGVSWRILLEDLQRAYADLD----ADL 1252

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1234 PPLPVQYADYAvwQRQWLQGETLNDLRDYWRDQLQGAPAllEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRL----HALN 1309
Cdd:PRK12316 1253 PARTSSYQAWA--RRLHEHAGARAEELDYWQAQLEDAPH--ELPCENPDGALENRHERKLELRLDAERTRQLlqeaPAAY 1328

                        1370      1380      1390
                  ....*....|....*....|....*....|
gi 641744967 1310 RQQGTTLFMTLLAAwsvVLSRLSGQDDIVI 1339
Cdd:PRK12316 1329 RTQVNDLLLTALAR---VTCRWSGQASVLV 1355
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
 1531-2021 0e+00

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 745.64  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDP 1610
Cdd:cd17646     1 HALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1611 GYPAERLAYMLDDASPVALLTQANQRALLTGDVPRILLDTADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMNS 1690
Cdd:cd17646    81 GYPADRLAYMLADAGPAVVLTTADLAARLPAGGDVALLGDEALAAPPATPPLVP-PRPDNLAYVIYTSGSTGRPKGVMVT 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1691 HRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSM 1770
Cdd:cd17646   160 HAGIVNRLLWMQDEYPLGPGDRVLQKTPLSFDVSVWELFWPLVAGARLVVARPGGHRDPAYLAALIREHGVTTCHFVPSM 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1771 LQQFVQWADADcACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHRSFVPIGRPIANTQ 1850
Cdd:cd17646   240 LRVFLAEPAAG-SCASLRRVFCSGEALPPELAARFLALPGAELHNLYGPTEAAIDVTHWPVRGPAETPSVPIGRPVPNTR 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1851 LYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQVK 1930
Cdd:cd17646   319 LYVLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAERFVPDPF--GPGSRMYRTGDLARWRPDGALEFLGRSDDQVK 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1931 LRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVT-PDPADLRQQLGQHLAEYMVPGAFVTLDAFP 2009
Cdd:cd17646   397 IRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGAAgPDTAALRAHLAERLPEYMVPAAFVVLDALP 476

                         490
                  ....*....|..
gi 641744967 2010 LTPNGKLDRKAL 2021
Cdd:cd17646   477 LTANGKLDRAAL 488
PRK12467 PRK12467
peptide synthase; Provisional
 30-1396 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 740.05  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGlHQPVQVVWRQAPLTVNTL 109
Cdd:PRK12467 1118 PLSYAQERQWFLWQLEPGSAAYHIPQALRLKGPLDIEALERSFDALVARHESLRTTFVQED-GRTRQVIHPVGSLTLEEP 1196

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  110 TTTSSDTVPAQLRAATDPSNHRL-NLSNAPLL------SATTAHdpvcgewLLSLSIHHLISDHITQALIIDEI----RL 178
Cdd:PRK12467 1197 LLLAADKDEAQLKVYVEAEARQPfDLEQGPLLrvgllrLAADEH-------VLVLTLHHIVSDGWSMQVLVDELvalyAA 1269

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  179 LLEDRPEALPK-PLPYRNF-IAQILSVPLSEHEQ---YFRNRLADiDTPT--APFDLVDVQGNGEDITEARLSLDSSLAD 251
Cdd:PRK12467 1270 YSQGQSLQLPAlPIQYADYaVWQRQWMDAGERARqlaYWKAQLGG-EQPVleLPTDRPRPAVQSHRGARLAFELPPALAE 1348

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  252 ALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQgnLGADRVMGMFINTLPLRVSLR-ERSVHDVVQAT 330
Cdd:PRK12467 1349 GLRALARREGVTLFMLLLASFQTLLHRYSGQDDIRVGVPIANRNR--AETEGLIGFFVNTQVLRAEVDgQASFQQLLQQV 1426

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  331 SHELMMLLAHEQAP---LALAQQCSQVPPPLPLFSTLFNYRHSQKDASSQFWE-GMRQLSGRERT-NYPITLSVDDLGDG 405
Cdd:PRK12467 1427 KQAALEAQAHQDLPfeqLVEALQPERSLSHSPLFQVMFNHQRDDHQAQAQLPGlSVESLSWESQTaQFDLTLDTYESSEG 1506

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  406 fnLTAKTVMGVD------PERIV-HYMltaieNLVTSLEKTPQQPALSQPILPKSERQQVLVDFNATDADFPREMLIQQR 478
Cdd:PRK12467 1507 --LQASLTYATDlfeastIERLAgHWL-----NLLQGLVADPERRLGELDLLDEAERRQILEGWNATHTGYPLARLVHQL 1579

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  479 FEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:PRK12467 1580 IEDQAAATPEAVALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYP 1659

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTQSAQVAQLNST--LPTVLLDTPAA--AACPDTNPVVQgLHAAHLAYVIYTSGSTGRPKGVMV 634
Cdd:PRK12467 1660 RERLAYMIEDSGIELLLTQSHLQARLPLPdgLRSLVLDQEDDwlEGYSDSNPAVN-LAPQNLAYVIYTSGSTGRPKGAGN 1738

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  635 AHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWI-QLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPV 713
Cdd:PRK12467 1739 RHGALVNRLCATQEAYQLSAADVVLQFTSFAFDVSVWELFwPLINGARLVIAPPGAHRDPEQLIQLIERQQVTTLHFVPS 1818

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  714 QLQWLLDAglgsDPAYQPA----QVLIGGEAISPAVWSR-LQSLSDTRFINVYGPTECTVDATACVVDRTQP-----LPt 783
Cdd:PRK12467 1819 MLQQLLQM----DEQVEHPlslrRVVCGGEALEVEALRPwLERLPDTGLFNLYGPTETAVDVTHWTCRRKDLegrdsVP- 1893

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  784 IGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsADPAARIYKTGDLARWLPDGNIDY 863
Cdd:PRK12467 1894 IGQPIANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPF-GTVGSRLYRTGDLARYRADGVIEY 1972

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  864 LGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIArEDSPGDTRLVAYLC----ARPDAELHPAA----LRQQLAASL 935
Cdd:PRK12467 1973 LGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIA-QDGANGKQLVAYVVptdpGLVDDDEAQVAlraiLKNHLKASL 2051

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  936 ADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAFaTRDYEAPQGGIETALAALWQELLGLDRVGRHDQFFALGGHSLL 1015
Cdd:PRK12467 2052 PEYMVPAHLVFLARMPLTPNGKLDRKALPAPDASEL-QQAYVAPQSELEQRLAAIWQDVLGLEQVGLHDNFFELGGDSII 2130

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1016 AVQLLNRMNKAGMDVALATLFAHPTLCDLAAavTATAHDAPFTLPVADRTQPLPLSfSQQRLWFlaQLDPAASQAYHLPA 1095
Cdd:PRK12467 2131 SIQVVSRARQAGIRFTPKDLFQHQTVQSLAA--VAQEGDGTVSIDQGPVTGDLPLL-PIQQMFF--ADDIPERHHWNQSV 2205

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1096 ALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQ-PAQQIDPDTLGFS-LSSHDLRKLDEaarttrVAELAEQeARA 1173
Cdd:PRK12467 2206 LLEPREALDAELLEAALQALLVHHDALRLGFVQEDGGwSAMHRAPEQERRPlLWQVVVADKEE------LEALCEQ-AQR 2278

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1174 RFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQYADYAVWQRQWLQG 1253
Cdd:PRK12467 2279 SLDLEEGPLLRAVLATLPDGSQRLLLVIHHLVVDGVSWRILLEDLQTAYRQLQGGQPVKLPAKTSAFKAWAERLQTYAAS 2358

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1254 ETLNDLRDYWRDQLQGAPAllEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLhalnRQQGTTLFMT-----LLAAWSVVL 1328
Cdd:PRK12467 2359 AALADELGYWQAQLQGAST--ELPCDHPQGGLQRRHAASVTTHLDSEWTRRL----LQEAPAAYRTqvndlLLTALARVI 2432

                        1370      1380      1390      1400      1410      1420      1430
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967 1329 SRLSGQDDIVIGTPVANR----PRQELEGMVGFFVNTLALRTEPGRchAVADLLDQVRERaLDAYAHQALPF 1396
Cdd:PRK12467 2433 ARWTGQASTLIQLEGHGRedlfDEIDLTRTVGWFTSLYPVKLSPTA--SLATSIKTIKEQ-LRAVPNKGLGF 2501
PRK12316 PRK12316
peptide synthase; Provisional
 2-1050 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 736.00  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    2 PLVTLSQEEIDTViatvPDGVANVQDIYPLAPLQEGILFHYQLQEKGDTYLlNSLLAFDSQTRLDAFLDVLQQVIARHDI 81
Cdd:PRK12316 4080 PLAGLDQARLDAL----PLPLGEIEDIYPLSPMQQGMLFHSLYEQEAGDYI-NQMRVDVQGLDVERFRAAWQAALDRHDV 4154

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   82 LRTAICWQG-LHQPVQVVWRQAPLTVNTLTTTSSDTVPAQLRAATDPSNHR-LNLSNAPLLSATTAHDPVcGEWLLSLSI 159
Cdd:PRK12316 4155 LRSGFVWQGeLGRPLQVVHKQVSLPFAELDWRGRADLQAALDALAAAERERgFDLQRAPLLRLVLVRTAE-GRHHLIYTN 4233

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  160 HHLISDHITQALIIDEIRLLLEDRPEALPKpLPYRNFIAQILSVPLSEHEQYFRNRLADIDTPT------APFDLVDVQG 233
Cdd:PRK12316 4234 HHILMDGWSNSQLLGEVLERYSGRPPAQPG-GRYRDYIAWLQRQDAAASEAFWREQLAALDEPTrlaqaiARADLRSANG 4312

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  234 NGEDITEarlsLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGADRVMGMFINTLP 313
Cdd:PRK12316 4313 YGEHVRE----LDATATARLREFARTQRVTLNTLVQAAWLLLLQRYTGQDTVAFGATVAGRPAELPGIEGQIGLFINTLP 4388

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  314 LRVSLR-ERSVHDVVQATSHELMMLLAHEQAPLALAQQCSQVPPPlPLFSTLFNYR------HSQKDASsqfwEGMR--Q 384
Cdd:PRK12316 4389 VIATPRaQQSVVEWLQQVQRQNLALREHEHTPLYEIQRWAGQGGE-ALFDSLLVFEnypvseALQQGAP----GGLRfgE 4463

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  385 LSGRERTNYPITLSVDdLGDGFNLTAKTVMGVDPERIVHYMLTAIENLVTSLEKTPQQPALSQPILPKSERQQVLVDFNA 464
Cdd:PRK12316 4464 VTNHEQTNYPLTLAVG-LGETLSLQFSYDRGHFDAATIERLARHLTNLLEAMAEDPQRRLGELQLLEKAEQQRIVALWNR 4542

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  465 TDADFPREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGIL 544
Cdd:PRK12316 4543 TDAGYPATRCVHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVL 4622

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  545 KAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLNST--LPTVLLD-TPAAAACPDTNPVVQgLHAAHLAYVIY 621
Cdd:PRK12316 4623 KAGGAYVPLDPEYPRERLAYMMEDSGAALLLTQSHLLQRLPIPdgLASLALDrDEDWEGFPAHDPAVR-LHPDNLAYVIY 4701

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  622 TSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQLLSGHTLVLVPDALRADAHQLWRYFA 701
Cdd:PRK12316 4702 TSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSFDGSHEGLYHPLINGASVVIRDDSLWDPERLYAEIH 4781

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  702 RHAVDLFDCTPVQLQWLL-DAGLGSDPAyQPAQVLIGGEAISPAVWSR-LQSLSDTRFINVYGPTECTVDATA-CVVDRT 778
Cdd:PRK12316 4782 EHRVTVLVFPPVYLQQLAeHAERDGEPP-SLRVYCFGGEAVAQASYDLaWRALKPVYLFNGYGPTETTVTVLLwKARDGD 4860

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  779 QPLPT---IGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSAdPAARIYKTGDLARW 855
Cdd:PRK12316 4861 ACGAAympIGTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVARGYLERPALTAERFVPDPFGA-PGGRLYRTGDLARY 4939

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  856 LPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGdTRLVAYLC----ARPDAELHPAALRQQ- 930
Cdd:PRK12316 4940 RADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAVG-KQLVGYVVpqdpALADADEAQAELRDEl 5018

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  931 ---LAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAfATRDYEAPQGGIETALAALWQELLGLDRVGRHDQFF 1007
Cdd:PRK12316 5019 kaaLRERLPEYMVPAHLVFLARMPLTPNGKLDRKALPQPDASL-LQQAYVAPRSELEQQVAAIWAEVLQLERVGLDDNFF 5097

                        1050      1060      1070      1080
                  ....*....|....*....|....*....|....*....|....
gi 641744967 1008 ALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAVTA 1050
Cdd:PRK12316 5098 ELGGHSLLAIQVTSRIQLElGLELPLRELFQTPTLAAFVELAAA 5141
PRK12316 PRK12316
peptide synthase; Provisional
 30-1369 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 734.46  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHqpvqvvWRQAPLTVNTL 109
Cdd:PRK12316 2604 PLSHAQQRQWFLWQLEPESAAYHLPSALHLRGVLDQAALEQAFDALVLRHETLRTRFVEVGEQ------TRQVILPNMSL 2677

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  110 TTTSSDTV---PAQLRAATDPSNHR-LNLSNAPLLSATTAhDPVCGEWLLSLSIHHLISDHITQALIIDEIRLLLE---- 181
Cdd:PRK12316 2678 RIVLEDCAgvaDAAIRQRVAEEIQRpFDLARGPLLRVRLL-ALDGQEHVLVITQHHIVSDGWSMQVMVDELVQAYAgarr 2756

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  182 -DRPEALPKPLPYRNFIAQ----ILSVPLSEHEQYFRNRLADIDTPTA-PFDLVDVQGNGEDITEARLSLDSSLADALRR 255
Cdd:PRK12316 2757 gEQPTLPPLPLQYADYAAWqrawMDSGEGARQLDYWRERLGGEQPVLElPLDRPRPALQSHRGARLDVALDVALSRELLA 2836

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  256 QARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRlqGNLGADRVMGMFINTLPLRVSLR-ERSVHDVVQATSHEL 334
Cdd:PRK12316 2837 LARREGVTLFMLLLASFQVLLHRYSGQSDIRVGVPIANR--NRAETERLIGFFVNTQVLRAQVDaQLAFRDLLGQVKEQA 2914

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  335 MMLLAHEQAP---LALAQQCSQVPPPLPLFSTLFNYRHSQKDASSQ---FWEGMRQLSGRERTNYPITLSVDDLGDGFNL 408
Cdd:PRK12316 2915 LGAQAHQDLPfeqLVEALQPERSLSHSPLFQVMYNHQSGERAAAQLpglHIESFAWDGAATQFDLALDTWESAEGLGASL 2994

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  409 TAKTVMGvdPERIVHYMLTAIENLVTSLEKTPQQPALSQPILPKSERQQVLVDFNATDADFPREMLIQQRFEAQAEQTPE 488
Cdd:PRK12316 2995 TYATDLF--DARTVERLARHWQNLLRGMVENPQRSVDELAMLDAEERGQLLEAWNATAAEYPLERGVHRLFEEQVERTPD 3072

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  489 AIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDD 568
Cdd:PRK12316 3073 AVALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLED 3152

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  569 AAPVALLTQSAQVAQLNSTLPTVLLDtPAAAACPDTNPVVQGLhAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHT 648
Cdd:PRK12316 3153 SGAQLLLSQSHLRLPLAQGVQVLDLD-RGDENYAEANPAIRTM-PENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQ 3230

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  649 LLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLdAGLGSDP 727
Cdd:PRK12316 3231 AYGLGVGDRVLQFTTFSFDVFVEElFWPLMSGARVVLAGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFL-EEEDAHR 3309

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  728 AYQPAQVLIGGEAISPAVWSRLqsLSDTRFINVYGPTECTVDATAC-VVDRTQPLPTIGKPLANTRLYILDAQDQPVPIG 806
Cdd:PRK12316 3310 CTSLKRIVCGGEALPADLQQQV--FAGLPLYNLYGPTEATITVTHWqCVEEGKDAVPIGRPIANRACYILDGSLEPVPVG 3387

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  807 VTGELHIGGAGVARGYLHRPDLTAERFIPDPFSadPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESR 886
Cdd:PRK12316 3388 ALGELYLGGEGLARGYHNRPGLTAERFVPDPFV--PGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEAR 3465

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  887 LLRCPGVQDAVVIAREDSpgdtRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAP 966
Cdd:PRK12316 3466 LLEHPWVREAVVLAVDGR----QLVAYVVPEDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRP 3541

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  967 DqTAFATRDYEAPQGGIETALAALWQELLGLDRVGRHDQFFALGGHSLLAVQLLNRMNKAGMDVALATLFAHPTLCDLAA 1046
Cdd:PRK12316 3542 D-AALLQQDYVAPVNELERRLAAIWADVLKLEQVGLTDNFFELGGDSIISLQVVSRARQAGIRFTPKDLFQHQTIQGLAR 3620

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1047 AVTATAHDAPFTLPV-------ADRTQPLPLSFSQQRLWFLAQLdpaasqayhlpaaLHLTGRLDRPALTTALNGLVARH 1119
Cdd:PRK12316 3621 VARVGGGVAVDQGPVsgetlllPIQQQFFEEPVPERHHWNQSLL-------------LKPREALDAAALEAALQALVEHH 3687

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1120 ESLRTRFTSIDGQPAQQIDPDTLGFSLSSHdlRKLDEAARTTRVAElaeqEARARFDLTQGPLIRGQLLQLDDNTHVLLL 1199
Cdd:PRK12316 3688 DALRLRFVEDAGGWTAEHLPVELGGALLWR--AELDDAEELERLGE----EAQRSLDLADGPLLRALLATLADGSQRLLL 3761

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1200 TQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPAllEIPTD 1279
Cdd:PRK12316 3762 VIHHLVVDGVSWRILLEDLQQAYQQLLQGEAPRLPAKTSSFKAWAERLQEHARGEALKAELAYWQEQLQGVSS--ELPCD 3839

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1280 RPRPSVQRYAGDQVPFHLDAGQLRRL----HALNRQQGTTLFMTLLAAwsvVLSRLSGQDDIVIGTPVANRPRQ----EL 1351
Cdd:PRK12316 3840 HPQGALQNRHAASVQTRLDRELTRRLlqqaPAAYRTQVNDLLLTALAR---VVCRWTGEASALVQLEGHGREDLfadiDL 3916

                        1370
                  ....*....|....*...
gi 641744967 1352 EGMVGFFVNTLALRTEPG 1369
Cdd:PRK12316 3917 SRTVGWFTSLFPVRLSPV 3934
PRK12316 PRK12316
peptide synthase; Provisional
 2129-3191 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 727.14  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2129 ITPDLLPLVTLTQPEIDRITdtVSGGAsnIQDIYPLAPLQEGILFHHLLQEQGDTYL--LRSMVafthrERLD--AFLSA 2204
Cdd:PRK12316 1528 VTPSDFPLAGLSQAQLDALP--LPAGE--IADIYPLSPMQQGMLFHSLYEQEAGDYInqLRVDV-----QGLDpdRFRAA 1598

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2205 LQQVIDRHDILRTAVCWQD-LSQPVQVVWRQAILPINHFEPTSPEDVLAQLQAHTEP-RTRRIDLSQAPLFRADIAHDPl 2282
Cdd:PRK12316 1599 WQATVDRHEILRSGFLWQDgLEQPLQVIHKQVELPFAELDWRGREDLGQALDALAQAeRQKGFDLTRAPLLRLVLVRTG- 1677

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2283 QNEWLLALSFHHLISDHMTLALIVGEIrllLQHQADALP--TPLPYRNFIAQTLSVPNSAHEAYFRDKLADVDEPTAPFG 2360
Cdd:PRK12316 1678 EGRHHLIYTNHHILMDGWSNAQLLGEV---LQRYAGQPVaaPGGRYRDYIAWLQRQDAAASEAFWKEQLAALEEPTRLAQ 1754

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2361 LLNVQGSGGDIHEARLVLDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADRIMGM 2440
Cdd:PRK12316 1755 AARTEDGQVGYGDHQQLLDPAQTRALAEFARAQKVTLNTLVQAAWLLLLQRYTGQETVAFGATVAGRPAELPGIEQQIGL 1834

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2441 FINTLPLRIS-LADRGAAEVVERTSHDLMTLLEHEQAPLALAQRCSGVAPPmPLFSTLL---NYRHTQA--SSTDNTLSD 2514
Cdd:PRK12316 1835 FINTLPVIAApRPDQSVADWLQEVQALNLALREHEHTPLYDIQRWAGQGGE-ALFDSLLvfeNYPVAEAlkQGAPAGLVF 1913

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2515 IRVlTSEERTNYPLTLAVDdRGEGFSL---------VAQTLEDIDPHrLLNylmtaissLVDALETEPQRSILNLPVLPD 2585
Cdd:PRK12316 1914 GRV-SNHEQTNYPLTLAVT-LGETLSLqysydrghfDAAAIERLDRH-LLH--------LLEQMAEDAQAALGELALLDA 1982

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2586 SERQQMLVDFNATDADIPRHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVER 2665
Cdd:PRK12316 1983 GERQRILADWDRTPEAYPRGPGVHQRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAER 2062

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2666 GLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQSAHLG--IMNGSLPVILLDdgetrPFDNEPDTPLD 2743
Cdd:PRK12316 2063 SFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLTQRHLLErlPLPAGVARLPLD-----RDAEWADYPDT 2137

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2744 ARKQGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLIL 2823
Cdd:PRK12316 2138 APAVQLAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSFSFDGAHEQWFHPLLNGARVLI 2217

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2824 ATQAQAADAQQLAMlIERHAVSFMQATPSTWRMLVEL--RDFALPPGFKALCGGEALPENLATALLQKVTT--LWNLYGP 2899
Cdd:PRK12316 2218 RDDELWDPEQLYDE-MERHGVTILDFPPVYLQQLAEHaeRDGRPPAVRVYCFGGEAVPAASLRLAWEALRPvyLFNGYGP 2296

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2900 TETTIWSTL------NGLTTPTPYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFS 2973
Cdd:PRK12316 2297 TEAVVTPLLwkcrpqDPCGAAYVPIGRALGNRRAYILDADLNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPDPFS 2376

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2974 gAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAReDSPGDKRLVAYLLAQPD 3053
Cdd:PRK12316 2377 -ASGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVVAQ-DGASGKQLVAYVVPDDA 2454

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3054 TVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPDQSAmATRGYEAPQGDLEHALAQIWQTLLGVER 3133
Cdd:PRK12316 2455 AEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQ-LRQAYVAPQEGLEQRLAAIWQAVLKVEQ 2533

                        1050      1060      1070      1080      1090
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 3134 VGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILAAQIHAA 3191
Cdd:PRK12316 2534 VGLDDHFFELGGHSLLATQVVSRVRQDLGLEVPLRILFERPTLAAFAASLESGQTSRA 2591
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
 1542-2021 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 703.90  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:cd05930     1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLTQANqralltgdvprilldtadfshlsednphvpgldahHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:cd05930    81 EDSGAKLVLTDPD-----------------------------------DLAYVIYTSGSTGKPKGVMVEHRGLVNLLLWM 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADaD 1781
Cdd:cd05930   126 QEAYPLTPGDRVLQFTSFSFDVSVWEIFGALLAGATLVVLPEEVRKDPEALADLLAEEGITVLHLTPSLLRLLLQELE-L 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1782 CACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDD-HRSFVPIGRPIANTQLYILDTLGQ 1859
Cdd:cd05930   205 AALPSLRLVLVGGEALPPDLVRRWRELLpGARLVNLYGPTEATVDATYYRVPPDDeEDGRVPIGRPIPNTRVYVLDENLR 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1860 PVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFInqPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELG 1939
Cdd:cd05930   285 PVPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPFG--PGERMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELG 362

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1940 EIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRK 2019
Cdd:cd05930   363 EIEAALLAHPGVREAAVVAREDGDGEKRLVAYVVPDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRK 442


                  ..
gi 641744967 2020 AL 2021
Cdd:cd05930   443 AL 444
E-C_NRPS cd19544
Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); ...
 2161-2569 0e+00

Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); Dual function Epimerization/Condensation (E/C) domains have both an epimerization and a DCL condensation activity. Dual E/C domains first epimerize the substrate amino acid to produce a D-configuration, then catalyze the condensation between the D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. They are D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. These Dual E/C domains contain an extended His-motif (HHx(N)GD) near the N-terminus of the domain in addition to the standard Condensation (C) domain active site motif (HHxxxD). C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains, these include the DCL-type, LCL-type, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C domains, and the X-domain.


Pssm-ID: 380466 [Multi-domain]  Cd Length: 413  Bit Score: 701.50  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2161 IYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILPIN 2240
Cdd:cd19544     1 IYPLAPLQEGILFHHLLAEEGDPYLLRSLLAFDSRARLDAFLAALQQVIDRHDILRTAILWEGLSEPVQVVWRQAELPVE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2241 HFEPTSPEDVLAQLQAHTEPRTRRIDLSQAPLFRADIAHDPLQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADAL 2320
Cdd:cd19544    81 ELTLDPGDDALAQLRARFDPRRYRLDLRQAPLLRAHVAEDPANGRWLLLLLFHHLISDHTSLELLLEEIQAILAGRAAAL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2321 PTPLPYRNFIAQTLSVPNSA-HEAYFRDKLADVDEPTAPFGLLNVQGSGGDIHEARLVLDATLASAIRQQARHLGVSPGV 2399
Cdd:cd19544   161 PPPVPYRNFVAQARLGASQAeHEAFFREMLGDVDEPTAPFGLLDVQGDGSDITEARLALDAELAQRLRAQARRLGVSPAS 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2400 LFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADRIMGMFINTLPLRISLADRGAAEVVERTSHDLMTLLEHEQAPLA 2479
Cdd:cd19544   241 LFHLAWALVLARCSGRDDVVFGTVLSGRMQGGAGADRALGMFINTLPLRVRLGGRSVREAVRQTHARLAELLRHEHASLA 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2480 LAQRCSGVAPPMPLFSTLLNYRHTQASSTDN---TLSDIRVLTSEERTNYPLTLAVDDRGEGFSLVAQTLEDIDPHRLLN 2556
Cdd:cd19544   321 LAQRCSGVPAPTPLFSALLNYRHSAAAAAAAalaAWEGIELLGGEERTNYPLTLSVDDLGDGFSLTAQVVAPIDAERVCA 400

                         410
                  ....*....|...
gi 641744967 2557 YLMTAISSLVDAL 2569
Cdd:cd19544   401 YMETALEQLVDAL 413
LCL_NRPS-like cd19531
LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar ...
 1067-1494 0e+00

LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar domains including the C-domain of SgcC5, a free-standing NRPS with both ester- and amide- bond forming activity; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. Streptomyces globisporus SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380454 [Multi-domain]  Cd Length: 427  Bit Score: 678.31  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1067 PLPLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDtLGFSL 1146
Cdd:cd19531     1 PLPLSFAQQRLWFLDQLEPG-SAAYNIPGALRLRGPLDVAALERALNELVARHEALRTTFVEVDGEPVQVILPP-LPLPL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1147 SSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAAL 1226
Cdd:cd19531    79 PVVDLSGLPEAEREAEAQRLAREEARRPFDLARGPLLRATLLRLGEDEHVLLLTMHHIVSDGWSMGVLLRELAALYAAFL 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1227 EGSEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLH 1306
Cdd:cd19531   159 AGRPSPLPPLPIQYADYAVWQREWLQGEVLERQLAYWREQLAGAPPVLELPTDRPRPAVQSFRGARVRFTLPAELTAALR 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1307 ALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERAL 1386
Cdd:cd19531   239 ALARREGATLFMTLLAAFQVLLHRYSGQDDIVVGTPVAGRNRAELEGLIGFFVNTLVLRTDLSGDPTFRELLARVRETAL 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1387 DAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQALTLPDLTLSAVERPQHSTHFDLSLSLIETENGLNGGLV 1466
Cdd:cd19531   319 EAYAHQDLPFEKLVEALQPERDLSRSPLFQVMFVLQNAPAAALELPGLTVEPLEVDSGTAKFDLTLSLTETDGGLRGSLE 398

                         410       420
                  ....*....|....*....|....*...
gi 641744967 1467 YATDLFDRETILRVVGYVENILMAMADD 1494
Cdd:cd19531   399 YNTDLFDAATIERMAGHFQTLLEAIVAD 426
E-C_NRPS cd19544
Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); ...
 28-436 0e+00

Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); Dual function Epimerization/Condensation (E/C) domains have both an epimerization and a DCL condensation activity. Dual E/C domains first epimerize the substrate amino acid to produce a D-configuration, then catalyze the condensation between the D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. They are D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. These Dual E/C domains contain an extended His-motif (HHx(N)GD) near the N-terminus of the domain in addition to the standard Condensation (C) domain active site motif (HHxxxD). C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains, these include the DCL-type, LCL-type, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C domains, and the X-domain.


Pssm-ID: 380466 [Multi-domain]  Cd Length: 413  Bit Score: 674.15  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   28 IYPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHQPVQVVWRQAPLTVN 107
Cdd:cd19544     1 IYPLAPLQEGILFHHLLAEEGDPYLLRSLLAFDSRARLDAFLAALQQVIDRHDILRTAILWEGLSEPVQVVWRQAELPVE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  108 TLTTTSSDTVPAQLRAATDPSNHRLNLSNAPLLSATTAHDPVCGEWLLSLSIHHLISDHITQALIIDEIRLLLEDRPEAL 187
Cdd:cd19544    81 ELTLDPGDDALAQLRARFDPRRYRLDLRQAPLLRAHVAEDPANGRWLLLLLFHHLISDHTSLELLLEEIQAILAGRAAAL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  188 PKPLPYRNFIAQILSVPLS-EHEQYFRNRLADIDTPTAPFDLVDVQGNGEDITEARLSLDSSLADALRRQARHLGISSSV 266
Cdd:cd19544   161 PPPVPYRNFVAQARLGASQaEHEAFFREMLGDVDEPTAPFGLLDVQGDGSDITEARLALDAELAQRLRAQARRLGVSPAS 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  267 LFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGADRVMGMFINTLPLRVSLRERSVHDVVQATSHELMMLLAHEQAPLA 346
Cdd:cd19544   241 LFHLAWALVLARCSGRDDVVFGTVLSGRMQGGAGADRALGMFINTLPLRVRLGGRSVREAVRQTHARLAELLRHEHASLA 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  347 LAQQCSQVPPPLPLFSTLFNYRHSQK---DASSQFWEGMRQLSGRERTNYPITLSVDDLGDGFNLTAKTVMGVDPERIVH 423
Cdd:cd19544   321 LAQRCSGVPAPTPLFSALLNYRHSAAaaaAAALAAWEGIELLGGEERTNYPLTLSVDDLGDGFSLTAQVVAPIDAERVCA 400

                         410
                  ....*....|...
gi 641744967  424 YMLTAIENLVTSL 436
Cdd:cd19544   401 YMETALEQLVDAL 413
PRK12467 PRK12467
peptide synthase; Provisional
 2163-3187 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 673.41  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2163 PLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCwQDLSQPVQVVWRQAILPInHF 2242
Cdd:PRK12467   51 PLSYAQERQWFLWQLDPDSAAYNIPTALRLRGELDVSALRRAFDALVARHESLRTRFV-QDEEGFRQVIDASLSLTI-PL 128

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2243 E---PTSPEDVLAQLQAHTEPRTRR-IDLSQAPLFRADI---AHDplqnEWLLALSFHHLISDHMTLALIVGEIRLLL-- 2313
Cdd:PRK12467  129 DdlaNEQGRARESQIEAYINEEVARpFDLANGPLLRVRLlrlADD----EHVLVVTLHHIISDGWSMRVLVEELVQLYsa 204

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2314 --QHQADALPT-PLPYRNF-IAQTLSVPNSAHE---AYFRDKLAD---VDEPTAPFGLLNVQGSGGDIHEARLvlDATLA 2383
Cdd:PRK12467  205 ysQGREPSLPAlPIQYADYaIWQRSWLEAGERErqlAYWQEQLGGehtVLELPTDRPRPAVPSYRGARLRVDL--PQALS 282

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2384 SAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISLADRGA-AEVVER 2462
Cdd:PRK12467  283 AGLKALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANR--NRVETERLIGFFVNTQVLKAEVDPQASfLELLQQ 360

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2463 TSHDLMTLLEHEQAPL-----ALA-QRCSGVAPpmpLFSTLLNYRHTQASSTDNTLSDIRVLTSEE------RTNYPLTL 2530
Cdd:PRK12467  361 VKRTALGAQAHQDLPFeqlveALQpERSLSHSP---LFQVMFNHQNTATGGRDREGAQLPGLTVEElswarhTAQFDLAL 437

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2531 AVDDRGEG----FSLVAQTLEDIDPHRLLNYLMtaisSLVDALETEPQRSILNLPVLPDSERQQMLVDFNATDADIPRHA 2606
Cdd:PRK12467  438 DTYESAQGlwaaFTYATDLFEATTIERLATHWR----NLLEAIVAEPRRRLGELPLLDAEERARELVRWNAPATEYAPDC 513

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2607 lIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPL 2686
Cdd:PRK12467  514 -VHQLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPL 592

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2687 DPTYPVERLRYMLDDAKPVALISQSAHLGIMN--GSLPVILLDDG----ETRPFDNePDTPLDarkqgltPRHLAYVIYT 2760
Cdd:PRK12467  593 DPEYPQDRLAYMLDDSGVRLLLTQSHLLAQLPvpAGLRSLCLDEPadllCGYSGHN-PEVALD-------PDNLAYVIYT 664

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2761 SGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIE 2840
Cdd:PRK12467  665 SGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGATLHLLPPDCARDAEAFAALMA 744

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2841 RHAVSFMQATPSTWRMLVELRDFALPPGFKAL-CGGEALPENLATALLQKV--TTLWNLYGPTETTIWST-----LNGLT 2912
Cdd:PRK12467  745 DQGVTVLKIVPSHLQALLQASRVALPRPQRALvCGGEALQVDLLARVRALGpgARLINHYGPTETTVGVStyelsDEERD 824

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2913 TPTPYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsGAPEARMYKTGDLGRWLPD 2992
Cdd:PRK12467  825 FGNVPIGQPLANLGLYILDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPF-GADGGRLYRTGDLARYRAD 903

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2993 GTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIArEDSPGDKRLVAYLLaqPDTVLEPA-------DLRQRL 3065
Cdd:PRK12467  904 GVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLA-QPGDAGLQLVAYLV--PAAVADGAehqatrdELKAQL 980

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3066 SEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPDQSAMaTRGYEAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGG 3145
Cdd:PRK12467  981 RQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPKPDASAV-QATFVAPQTELEKRLAAIWADVLKVERVGLTDNFFELGG 1059

                        1050      1060      1070      1080
                  ....*....|....*....|....*....|....*....|..
gi 641744967 3146 HSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILAAQ 3187
Cdd:PRK12467 1060 HSLLATQVISRVRQRLGIQVPLRTLFEHQTLAGFAQAVAAQQ 1101
PRK12316 PRK12316
peptide synthase; Provisional
 2163-3191 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 631.61  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2163 PLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDlSQPVQVVWRQAILPINHf 2242
Cdd:PRK12316   51 RLSYAQQRMWFLWQLEPQSGAYNLPSAVRLNGPLDRQALERAFASLVQRHETLRTVFPRGA-DDSLAQVPLDRPLEVEF- 128

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2243 eptspEDVLAQLQAHTEPRTRR---------IDLSQAPLFRADIAHDPLQnEWLLALSFHHLISDHMTLALIVGE-IRLL 2312
Cdd:PRK12316  129 -----EDCSGLPEAEQEARLRDeaqreslqpFDLCEGPLLRVRLLRLGEE-EHVLLLTLHHIVSDGWSMNVLIEEfSRFY 202

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2313 LQHQADALP--TPLP-----YRNFIAQTLSVPNSAHE-AYFRDKLAD--------VDEPTAPfgllnVQGSGGDIHEarL 2376
Cdd:PRK12316  203 SAYATGAEPglPALPiqyadYALWQRSWLEAGEQERQlEYWRAQLGEehpvlelpTDHPRPA-----VPSYRGSRYE--F 275

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2377 VLDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAegADRIMGMFINTLPLRISLADRGA 2456
Cdd:PRK12316  276 SIDPALAEALRGTARRQGLTLFMLLLGAFNVLLHRYSGQTDIRVGVPIANRNRAE--VEGLIGFFVNTQVLRSVFDGRTR 353

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2457 -AEVVERTSHDLMTLLEHEQAP---LALAQRCSGVAPPMPLFSTLLNYRHTQAS-STDNTLSDIRVLTSEER---TNYPL 2528
Cdd:PRK12316  354 vATLLAGVKDTVLGAQAHQDLPferLVEALKVERSLSHSPLFQVMYNHQPLVADiEALDTVAGLEFGQLEWKsrtTQFDL 433

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2529 TLAVDDRGEGFSLVAQTLEDIDPHRLLNYLMTAISSLVDALETEPQRSILNLPVLPDSERQQMLVDFNATDADIPRHALI 2608
Cdd:PRK12316  434 TLDTYEKGGRLHAALTYATDLFEARTVERMARHWQNLLRGMVENPQARVDELPMLDAEERGQLVEGWNATAAEYPLQRGV 513

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2609 HELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDP 2688
Cdd:PRK12316  514 HRLFEEQVERTPEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDP 593

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2689 TYPVERLRYMLDDAKPVALISQSaHLGI---MNGSLPVILLDDGeTRPFDNEPDTPLDARkqgLTPRHLAYVIYTSGSTG 2765
Cdd:PRK12316  594 EYPAERLAYMLEDSGVQLLLSQS-HLGRklpLAAGVQVLDLDRP-AAWLEGYSEENPGTE---LNPENLAYVIYTSGSTG 668

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2766 KPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVS 2845
Cdd:PRK12316  669 KPKGAGNRHRALSNRLCWMQQAYGLGVGDTVLQKTPFSFDVSVWEFFWPLMSGARLVVAAPGDHRDPAKLVELINREGVD 748

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2846 FMQATPSTWRMLVELRDFALPPGFKAL-CGGEALPENLATALLQK--VTTLWNLYGPTETTI----WSTLNGLTTPTPyI 2918
Cdd:PRK12316  749 TLHFVPSMLQAFLQDEDVASCTSLRRIvCSGEALPADAQEQVFAKlpQAGLYNLYGPTEAAIdvthWTCVEEGGDSVP-I 827

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2919 GHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGApeARMYKTGDLGRWLPDGTLEYL 2998
Cdd:PRK12316  828 GRPIANLACYILDANLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVPSPFVAG--ERMYRTGDLARYRADGVIEYA 905

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2999 GRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREdspgDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAF 3078
Cdd:PRK12316  906 GRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVD----GKQLVGYVVLESEGGDWREALKAHLAASLPEYMVPAQW 981

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3079 VTLDAFPLTPNGKLDRKALPAPDQSaMATRGYEAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIR 3158
Cdd:PRK12316  982 LALERLPLTPNGKLDRKALPAPEAS-VAQQGYVAPRNALERTLAAIWQDVLGVERVGLDDNFFELGGDSIVSIQVVSRAR 1060

                        1050      1060      1070
                  ....*....|....*....|....*....|...
gi 641744967 3159 AEFLTDIPiVAIFQHPQLSALAEVILAAQIHAA 3191
Cdd:PRK12316 1061 QAGIQLSP-RDLFQHQTIRSLALVAKAGQATAA 1092
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
 487-963 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 622.63  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd05930     1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLTQsaqvaqlnstlptvlldtpaaaacpdtnpvvqglhAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd05930    81 EDSGAKLVLTD-----------------------------------PDDLAYVIYTSGSTGKPKGVMVEHRGLVNLLLWM 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 HTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGS 725
Cdd:cd05930   126 QEAYPLTPGDRVLQFTSFSFDVSVWEiFGALLAGATLVVLPEEVRKDPEALADLLAEEGITVLHLTPSLLRLLLQELELA 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  726 DPAyQPAQVLIGGEAISPAVWSRLQSL-SDTRFINVYGPTECTVDATACVVDRTQPL---PTIGKPLANTRLYILDAQDQ 801
Cdd:cd05930   206 ALP-SLRLVLVGGEALPPDLVRRWRELlPGARLVNLYGPTEATVDATYYRVPPDDEEdgrVPIGRPIPNTRVYVLDENLR 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  802 PVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsaDPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAG 881
Cdd:cd05930   285 PVPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPF--GPGERMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELG 362

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  882 EIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRK 961
Cdd:cd05930   363 EIEAALLAHPGVREAAVVAREDGDGEKRLVAYVVPDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRK 442


                  ..
gi 641744967  962 AL 963
Cdd:cd05930   443 AL 444
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
 2620-3097 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 613.38  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd05930     1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQsahlgimngslpvillddgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVN 2779
Cdd:cd05930    81 EDSGAKLVLTD----------------------------------------PDDLAYVIYTSGSTGKPKGVMVEHRGLVN 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVE 2859
Cdd:cd05930   121 LLLWMQEAYPLTPGDRVLQFTSFSFDVSVWEIFGALLAGATLVVLPEEVRKDPEALADLLAEEGITVLHLTPSLLRLLLQ 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2860 LRDFALPPGFK-ALCGGEALPENLATALLQKV--TTLWNLYGPTETTIWSTLNGLTTPTPY-----IGHPIANTQIYILD 2931
Cdd:cd05930   201 ELELAALPSLRlVLVGGEALPPDLVRRWRELLpgARLVNLYGPTEATVDATYYRVPPDDEEdgrvpIGRPIPNTRVYVLD 280

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2932 AQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFR 3011
Cdd:cd05930   281 ENLRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPF--GPGERMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYR 358

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3012 IELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGK 3091
Cdd:cd05930   359 IELGEIEAALLAHPGVREAAVVAREDGDGEKRLVAYVVPDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGK 438


                  ....*.
gi 641744967 3092 LDRKAL 3097
Cdd:cd05930   439 VDRKAL 444
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
 477-967 0e+00

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 605.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  477 QRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPA 556
Cdd:cd17655     1 ELFEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  557 YPAERLAYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTN--PVVQglhAAHLAYVIYTSGSTGRPKGVMV 634
Cdd:cd17655    81 YPEERIQYILEDSGADILLTQSHLQPPIAFIGLIDLLDEDTIYHEESENlePVSK---SDDLAYVIYTSGSTGKPKGVMI 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  635 AHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPV 713
Cdd:cd17655   158 EHRGVVNLVEWANKVIYQGEHLRVALFASISFDASVTEiFASLLSGNTLYIVRKETVLDGQALTQYIRQNRITIIDLTPA 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  714 QLQWLLDAGLGSDPAYQpaQVLIGGEAISPAV---WSRLQSLSdTRFINVYGPTECTVDATACVVD---RTQPLPTIGKP 787
Cdd:cd17655   238 HLKLLDAADDSEGLSLK--HLIVGGEALSTELakkIIELFGTN-PTITNAYGPTETTVDASIYQYEpetDQQVSVPIGKP 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  788 LANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSadPAARIYKTGDLARWLPDGNIDYLGRN 867
Cdd:cd17655   315 LGNTRIYILDQYGRPQPVGVAGELYIGGEGVARGYLNRPELTAEKFVDDPFV--PGERMYRTGDLARWLPDGNIEFLGRI 392

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  868 DFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCArpDAELHPAALRQQLAASLADYMIPSAFVTL 947
Cdd:cd17655   393 DHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVS--EKELPVAQLREFLARELPDYMIPSYFIKL 470

                         490       500
                  ....*....|....*....|
gi 641744967  948 DALPLTPNGKLDRKALPAPD 967
Cdd:cd17655   471 DEIPLTPNGKVDRKALPEPD 490
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
 1532-2025 0e+00

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 597.00  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1532 QLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:cd17655     1 ELFEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 YPAERLAYMLDDASPVALLTQANQRALL--TGDVprILLDTADFSHLSEDNPHVPGlDAHHLAYVIYTSGSTGKPKGVMN 1689
Cdd:cd17655    81 YPEERIQYILEDSGADILLTQSHLQPPIafIGLI--DLLDEDTIYHEESENLEPVS-KSDDLAYVIYTSGSTGKPKGVMI 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPS 1769
Cdd:cd17655   158 EHRGVVNLVEWANKVIYQGEHLRVALFASISFDASVTEIFASLLSGNTLYIVRKETVLDGQALTQYIRQNRITIIDLTPA 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1770 MLQQFVQwADADCACdSLRRVICSGEALPAELQQRFFARF--NAQLHNLYGPTEAAIDVTFWACQPDDHRS-FVPIGRPI 1846
Cdd:cd17655   238 HLKLLDA-ADDSEGL-SLKHLIVGGEALSTELAKKIIELFgtNPTITNAYGPTETTVDASIYQYEPETDQQvSVPIGKPL 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1847 ANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFInqPGARLYKTGDLARWLPDGSLEYLGRND 1926
Cdd:cd17655   316 GNTRIYILDQYGRPQPVGVAGELYIGGEGVARGYLNRPELTAEKFVDDPFV--PGERMYRTGDLARWLPDGNIEFLGRID 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1927 FQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTpdPADLRQQLGQHLAEYMVPGAFVTLD 2006
Cdd:cd17655   394 HQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKELP--VAQLREFLARELPDYMIPSYFIKLD 471

                         490
                  ....*....|....*....
gi 641744967 2007 AFPLTPNGKLDRKALPAPD 2025
Cdd:cd17655   472 EIPLTPNGKVDRKALPEPD 490
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
 1534-2022 0e+00

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 596.25  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1534 VEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYP 1613
Cdd:cd17651     1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1614 AERLAYMLDDASPVALLTQANQRALLTGDVPRILLDTADFSHLSEDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNSHRA 1693
Cdd:cd17651    81 AERLAFMLADAGPVLVLTHPALAGELAVELVAVTLLDQPGAAAGADAEPDPALDADDLAYVIYTSGSTGRPKGVVMPHRS 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1694 LCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQ 1773
Cdd:cd17651   161 LANLVAWQARASSLGPGARTLQFAGLGFDVSVQEIFSTLCAGATLVLPPEEVRTDPPALAAWLDEQRISRVFLPTVALRA 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1774 FVQWADADCA-CDSLRRVICSGEALP-AELQQRFFARF-NAQLHNLYGPTEAAIdVTFWA--CQPDDHRSFVPIGRPIAN 1848
Cdd:cd17651   241 LAEHGRPLGVrLAALRYLLTGGEQLVlTEDLREFCAGLpGLRLHNHYGPTETHV-VTALSlpGDPAAWPAPPPIGRPIDN 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1849 TQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFInqPGARLYKTGDLARWLPDGSLEYLGRNDFQ 1928
Cdd:cd17651   320 TRVYVLDAALRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPDPFV--PGARMYRTGDLARWLPDGELEFLGRADDQ 397

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1929 VKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAF 2008
Cdd:cd17651   398 VKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRLVAYVVGDPEAPVDAAELRAALATHLPEYMVPSAFVLLDAL 477

                         490
                  ....*....|....
gi 641744967 2009 PLTPNGKLDRKALP 2022
Cdd:cd17651   478 PLTPNGKLDRRALP 491
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
 2620-3097 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 591.96  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd12116     1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQSAHLGIMNGSLPVILLDDGETRPFDNEPDTPLDarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVN 2779
Cdd:cd12116    81 EDAEPALVLTDDALPDRLPAGLPVLLLALAAAAAAPAAPRTPVS-------PDDLAYVIYTSGSTGRPKGVVVSHRNLVN 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVE 2859
Cdd:cd12116   154 FLHSMRERLGLGPGDRLLAVTTYAFDISLLELLLPLLAGARVVIAPRETQRDPEALARLIEAHSITVMQATPATWRMLLD 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2860 lRDFALPPGFKALCGGEALPENLATALLQKVTTLWNLYGPTETTIWSTLNGLTTPTPY--IGHPIANTQIYILDAQGRVV 2937
Cdd:cd12116   234 -AGWQGRAGLTALCGGEALPPDLAARLLSRVGSLWNLYGPTETTIWSTAARVTAAAGPipIGRPLANTQVYVLDAALRPV 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2938 PLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGaPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEI 3017
Cdd:cd12116   313 PPGVPGELYIGGDGVAQGYLGRPALTAERFVPDPFAG-PGSRLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEI 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3018 ETRLARCHGVHDAVVIAREDsPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd12116   392 EAALAAHPGVAQAAVVVRED-GGDRRLVAYVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
 475-964 0e+00

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 589.41  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  475 IQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMD 554
Cdd:cd17644     2 IHQLFEEQVERTPDAVAVVFEDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPLD 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  555 PAYPAERLAYILDDAAPVALLTQSAqvaqlnstlptvlldtpaaaacpdtnpvvqglhaaHLAYVIYTSGSTGRPKGVMV 634
Cdd:cd17644    82 PNYPQERLTYILEDAQISVLLTQPE-----------------------------------NLAYVIYTSGSTGKPKGVMI 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  635 AHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQ-LLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPV 713
Cdd:cd17644   127 EHQSLVNLSHGLIKEYGITSSDRVLQFASIAFDVAAEEIYVtLLSGATLVLRPEEMRSSLEDFVQYIQQWQLTVLSLPPA 206

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  714 QLQWLLDAGLGS--DPAYQPAQVLIGGEAISPAVWSRLQSLS--DTRFINVYGPTECTVDATACVVDRTQPL----PTIG 785
Cdd:cd17644   207 YWHLLVLELLLStiDLPSSLRLVIVGGEAVQPELVRQWQKNVgnFIQLINVYGPTEATIAATVCRLTQLTERnitsVPIG 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  786 KPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSADPAARIYKTGDLARWLPDGNIDYLG 865
Cdd:cd17644   287 RPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELTAEKFISHPFNSSESERLYKTGDLARYLPDGNIEYLG 366

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  866 RNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFV 945
Cdd:cd17644   367 RIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYIVPHYEESPSTVELRQFLKAKLPDYMIPSAFV 446

                         490
                  ....*....|....*....
gi 641744967  946 TLDALPLTPNGKLDRKALP 964
Cdd:cd17644   447 VLEELPLTPNGKIDRRALP 465
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
 1532-2021 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 584.55  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1532 QLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:cd12117     1 ELFEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 YPAERLAYMLDDASPVALLTQANQRALLTGDVPRILLDTADFSHlSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMNSH 1691
Cdd:cd12117    81 LPAERLAFMLADAGAKVLLTDRSLAGRAGGLEVAVVIDEALDAG-PAGNPAVP-VSPDDLAYVMYTSGSTGRPKGVAVTH 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1692 RALcNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSML 1771
Cdd:cd12117   159 RGV-VRLVKNTNYVTLGPDDRVLQTSPLAFDASTFEIWGALLNGARLVLAPKGTLLDPDALGALIAEEGVTVLWLTAALF 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1772 QQFVQwADADCaCDSLRRVICSGEALPAELQQRFFARFNA-QLHNLYGPTEAAIDVTFWAC-QPDDHRSFVPIGRPIANT 1849
Cdd:cd12117   238 NQLAD-EDPEC-FAGLRELLTGGEVVSPPHVRRVLAACPGlRLVNGYGPTENTTFTTSHVVtELDEVAGSIPIGRPIANT 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1850 QLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQV 1929
Cdd:cd12117   316 RVYVLDEDGRPVPPGVPGELYVGGDGLALGYLNRPALTAERFVADPF--GPGERLYRTGDLARWLPDGRLEFLGRIDDQV 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1930 KLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVtpDPADLRQQLGQHLAEYMVPGAFVTLDAFP 2009
Cdd:cd12117   394 KIRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDKRLVAYVVAEGAL--DAAELRAFLRERLPAYMVPAAFVVLDELP 471

                         490
                  ....*....|..
gi 641744967 2010 LTPNGKLDRKAL 2021
Cdd:cd12117   472 LTANGKVDRRAL 483
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
 479-964 0e+00

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 576.99  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  479 FEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:cd17651     1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTQSA-QVAQLNSTLPTVLLDTPAAAACPDTNPVVqGLHAAHLAYVIYTSGSTGRPKGVMVAHR 637
Cdd:cd17651    81 AERLAFMLADAGPVLVLTHPAlAGELAVELVAVTLLDQPGAAAGADAEPDP-ALDADDLAYVIYTSGSTGRPKGVVMPHR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  638 NVINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQ 716
Cdd:cd17651   160 SLANLVAWQARASSLGPGARTLQFAGLGFDVSVQEiFSTLCAGATLVLPPEEVRTDPPALAAWLDEQRISRVFLPTVALR 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  717 WLLDAGlGSDPAYQPA--QVLIGGEA--ISPAVWSRLQSLSDTRFINVYGPTECTVdATACVVDRTQP----LPTIGKPL 788
Cdd:cd17651   240 ALAEHG-RPLGVRLAAlrYLLTGGEQlvLTEDLREFCAGLPGLRLHNHYGPTETHV-VTALSLPGDPAawpaPPPIGRPI 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  789 ANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSadPAARIYKTGDLARWLPDGNIDYLGRND 868
Cdd:cd17651   318 DNTRVYVLDAALRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPDPFV--PGARMYRTGDLARWLPDGELEFLGRAD 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  869 FQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLD 948
Cdd:cd17651   396 DQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRLVAYVVGDPEAPVDAAELRAALATHLPEYMVPSAFVLLD 475

                         490
                  ....*....|....*.
gi 641744967  949 ALPLTPNGKLDRKALP 964
Cdd:cd17651   476 ALPLTPNGKLDRRALP 491
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
 1542-2021 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 575.78  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:cd12116     1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLTQANQRALLTGDVPRILLDTADfSHLSEDNPHvPGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:cd12116    81 EDAEPALVLTDDALPDRLPAGLPVLLLALAA-AAAAPAAPR-TPVSPDDLAYVIYTSGSTGRPKGVVVSHRNLVNFLHSM 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFV--QWAD 1779
Cdd:cd12116   159 RERLGLGPGDRLLAVTTYAFDISLLELLLPLLAGARVVIAPRETQRDPEALARLIEAHSITVMQATPATWRMLLdaGWQG 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1780 AdcacDSLrRVICSGEALPAELQQRFFARfNAQLHNLYGPTEAaidvTFW--ACQPDDHRSFVPIGRPIANTQLYILDTL 1857
Cdd:cd12116   239 R----AGL-TALCGGEALPPDLAARLLSR-VGSLWNLYGPTET----TIWstAARVTAAAGPIPIGRPLANTQVYVLDAA 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1858 GQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIE 1937
Cdd:cd12116   309 LRPVPPGVPGELYIGGDGVAQGYLGRPALTAERFVPDPF-AGPGSRLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIE 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1938 LGEIEARLMQCPGVQEAVVVAREDsPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:cd12116   388 LGEIEAALAAHPGVAQAAVVVRED-GGDRRLVAYVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLD 466


                  ....
gi 641744967 2018 RKAL 2021
Cdd:cd12116   467 RKAL 470
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
 477-963 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 566.83  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  477 QRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPA 556
Cdd:cd12117     1 ELFEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  557 YPAERLAYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAAcPDTNPVVqGLHAAHLAYVIYTSGSTGRPKGVMVAH 636
Cdd:cd12117    81 LPAERLAFMLADAGAKVLLTDRSLAGRAGGLEVAVVIDEALDAG-PAGNPAV-PVSPDDLAYVMYTSGSTGRPKGVAVTH 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  637 RNVINLATGlHTLLALDHPSRIALNASIVFDASV-KNWIQLLSGHTLVLVPDALRADAHQLWRYFARHAVD-LFDCTPV- 713
Cdd:cd12117   159 RGVVRLVKN-TNYVTLGPDDRVLQTSPLAFDASTfEIWGALLNGARLVLAPKGTLLDPDALGALIAEEGVTvLWLTAALf 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  714 -QL-----QWLldAGLGsdpayqpaQVLIGGEAISPA-VWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPT--- 783
Cdd:cd12117   238 nQLadedpECF--AGLR--------ELLTGGEVVSPPhVRRVLAACPGLRLVNGYGPTENTTFTTSHVVTELDEVAGsip 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  784 IGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsaDPAARIYKTGDLARWLPDGNIDY 863
Cdd:cd12117   308 IGRPIANTRVYVLDEDGRPVPPGVPGELYVGGDGLALGYLNRPALTAERFVADPF--GPGERLYRTGDLARWLPDGRLEF 385

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  864 LGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLcaRPDAELHPAALRQQLAASLADYMIPSA 943
Cdd:cd12117   386 LGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDKRLVAYV--VAEGALDAAELRAFLRERLPAYMVPAA 463

                         490       500
                  ....*....|....*....|
gi 641744967  944 FVTLDALPLTPNGKLDRKAL 963
Cdd:cd12117   464 FVVLDELPLTANGKVDRRAL 483
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
 1542-2022 1.02e-180

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 562.26  E-value: 1.02e-180
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:cd17652     1 PDAPAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLTQANqralltgdvprilldtadfshlsednphvpgldahHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:cd17652    81 ADARPALLLTTPD-----------------------------------NLAYVIYTSGSTGRPKGVVVTHRGLANLAAAQ 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQqfvqWADAD 1781
Cdd:cd17652   126 IAAFDVGPGSRVLQFASPSFDASVWELLMALLAGATLVLAPAEELLPGEPLADLLREHRITHVTLPPAALA----ALPPD 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1782 CACDsLRRVICSGEALPAELQQRFFARfnAQLHNLYGPTEAAIDVTFWACQPDDHRsfVPIGRPIANTQLYILDTLGQPV 1861
Cdd:cd17652   202 DLPD-LRTLVVAGEACPAELVDRWAPG--RRMINAYGPTETTVCATMAGPLPGGGV--PPIGRPVPGTRVYVLDARLRPV 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1862 PLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEI 1941
Cdd:cd17652   277 PPGVPGELYIAGAGLARGYLNRPGLTAERFVADPF-GAPGSRMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEV 355

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1942 EARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd17652   356 EAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435


                  .
gi 641744967 2022 P 2022
Cdd:cd17652   436 P 436
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
 1530-2022 6.07e-180

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 561.29  E-value: 6.07e-180
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLD 1609
Cdd:cd17644     2 IHQLFEEQVERTPDAVAVVFEDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPLD 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1610 PGYPAERLAYMLDDASPVALLTQAnqralltgdvprilldtadfshlsednphvpgldaHHLAYVIYTSGSTGKPKGVMN 1689
Cdd:cd17644    82 PNYPQERLTYILEDAQISVLLTQP-----------------------------------ENLAYVIYTSGSTGKPKGVMI 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPS 1769
Cdd:cd17644   127 EHQSLVNLSHGLIKEYGITSSDRVLQFASIAFDVAAEEIYVTLLSGATLVLRPEEMRSSLEDFVQYIQQWQLTVLSLPPA 206

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1770 MLQQfvqWADA----DCAC-DSLRRVICSGEA-LPAELQQRFFA-RFNAQLHNLYGPTEAAIDVTFwaCQPDDHRSF--- 1839
Cdd:cd17644   207 YWHL---LVLElllsTIDLpSSLRLVIVGGEAvQPELVRQWQKNvGNFIQLINVYGPTEATIAATV--CRLTQLTERnit 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1840 -VPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINQPGARLYKTGDLARWLPDGS 1918
Cdd:cd17644   282 sVPIGRPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELTAEKFISHPFNSSESERLYKTGDLARYLPDGN 361

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1919 LEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMV 1998
Cdd:cd17644   362 IEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYIVPHYEESPSTVELRQFLKAKLPDYMI 441

                         490       500
                  ....*....|....*....|....
gi 641744967 1999 PGAFVTLDAFPLTPNGKLDRKALP 2022
Cdd:cd17644   442 PSAFVVLEELPLTPNGKIDRRALP 465
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
 1542-2021 8.09e-180

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 560.39  E-value: 8.09e-180
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:cd17643     1 PEAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLTqanqralltgdvprilldtadfshlsednphvpglDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:cd17643    81 ADSGPSLLLT-----------------------------------DPDDLAYVIYTSGSTGRPKGVVVSHANVLALFAAT 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADAD 1781
Cdd:cd17643   126 QRWFGFNEDDVWTLFHSYAFDFSVWEIWGALLHGGRLVVVPYEVARSPEDFARLLRDEGVTVLNQTPSAFYQLVEAADRD 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1782 C-ACDSLRRVICSGEALPAELQQRFFARFN---AQLHNLYGPTEAAIDVTFWACQPDD--HRSFVPIGRPIANTQLYILD 1855
Cdd:cd17643   206 GrDPLALRYVIFGGEALEAAMLRPWAGRFGldrPQLVNMYGITETTVHVTFRPLDAADlpAAAASPIGRPLPGLRVYVLD 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1856 TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFR 1935
Cdd:cd17643   286 ADGRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVANPF-GGPGSRMYRTGDLARRLPDGELEYLGRADEQVKIRGFR 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1936 IELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGK 2015
Cdd:cd17643   365 IELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGK 444


                  ....*.
gi 641744967 2016 LDRKAL 2021
Cdd:cd17643   445 LDRAAL 450
PRK05691 PRK05691
peptide synthase; Validated
 1069-2106 1.52e-177

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 617.57  E-value: 1.52e-177
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1069 PLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLGFSLSS 1148
Cdd:PRK05691 3259 PLTPMQEGLLLHTLLEPGTG-LYYMQDRYRINSALDPERFAQAWQAVVARHEALRASFSWNAGETMLQVIHKPGRTPIDY 3337

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1149 HDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEG 1228
Cdd:PRK05691 3338 LDWRGLPEDGQEQRLQALHKQEREAGFDLLNQPPFHLRLIRVDEARYWFMMSNHHILIDAWCRSLLMNDFFEIYTALGEG 3417

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1229 SEANLPPLPvQYADYAvwqrQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPrpSVQRYAGDQVPF-------HLDAGQ 1301
Cdd:PRK05691 3418 REAQLPVPP-RYRDYI----GWLQRQDLAQARQWWQDNLRGFERPTPIPSDRP--FLREHAGDSGGMvvgdcytRLDAAD 3490

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1302 LRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQ--ELEGMVGFFVNTLALRT---EPGRCHAVAD 1376
Cdd:PRK05691 3491 GARLRELAQAHQLTVNTFAQAAWALVLRRYSGDRDVLFGVTVAGRPVSmpQMQRTVGLFINSIALRVqlpAAGQRCSVRQ 3570

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1377 LLDQVRERALDAYAHQALPfeqVVEILQPARSLSYSPIFQVMLSLNNTPAQaltlpdltLSAVERPQH--------STHF 1448
Cdd:PRK05691 3571 WLQGLLDSNMELREYEYLP---LVAIQECSELPKGQPLFDSLFVFENAPVE--------VSVLDRAQSlnassdsgRTHT 3639

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1449 DLSLSLI---ETENGLNggLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQVMLDFNATEADFP 1525
Cdd:PRK05691 3640 NFPLTAVcypGDDLGLH--LSYDQRYFDAPTVERLLGEFKRLLLALVQGFHGDLSELPLLGEQERDFLLDGCNRSERDYP 3717

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1526 HDALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAY 1605
Cdd:PRK05691 3718 LEQSYVRLFEAQVAAHPQRIAASCLDQQWSYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLLGMIVGSFKAGAGY 3797

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1606 VPLDPGYPAERLAYMLD-DASPVALLTQA---NQRALLTG----DVPRILL-DTADFSHLSEDNPHVPGlDAHHLAYVIY 1676
Cdd:PRK05691 3798 LPLDPGLPAQRLQRIIElSRTPVLVCSAAcreQARALLDElgcaNRPRLLVwEEVQAGEVASHNPGIYS-GPDNLAYVIY 3876

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1677 TSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLI 1756
Cdd:PRK05691 3877 TSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQSFDISVWQFLAAPLFGARVEIVPNAIAHDPQGLLAHV 3956

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1757 ERTGITTLHFVPSMLQQFVqwADADCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDD 1835
Cdd:PRK05691 3957 QAQGITVLESVPSLIQGML--AEDRQALDGLRWMLPTGEAMPPELARQWLQRYpQIGLVNAYGPAECSDDVAFFRVDLAS 4034

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1836 HR-SFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWL 1914
Cdd:PRK05691 4035 TRgSYLPIGSPTDNNRLYLLDEALELVPLGAVGELCVAGTGVGRGYVGDPLRTALAFVPHPF-GAPGERLYRTGDLARRR 4113

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1915 PDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDtRLVAYLCPQPGVTpDPADLRQQLGQHL- 1993
Cdd:PRK05691 4114 SDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVAVQEGVNGK-HLVGYLVPHQTVL-AQGALLERIKQRLr 4191

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1994 ---AEYMVPGAFVTLDAFPLTPNGKLDRKALPAPDQSAVATRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGH 2070
Cdd:PRK05691 4192 aelPDYMVPLHWLWLDRLPLNANGKLDRKALPALDIGQLQSQAYLAPRNELEQTLATIWADVLKVERVGVHDNFFELGGH 4271

                        1050      1060      1070
                  ....*....|....*....|....*....|....*...
gi 641744967 2071 SLLIVSLIERLRRAgLALDV--RGVFSTPVLSDMAQAI 2106
Cdd:PRK05691 4272 SLLATQIASRVQKA-LQRNVplRAMFECSTVEELAEYI 4308
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
2163-3191 6.63e-177

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 583.93  E-value: 6.63e-177
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2163 PLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCwQDLSQPVQVVWRQAILPINHF 2242
Cdd:PRK10252    9 PLVAAQPGIWMAEKLSPLPSAWSVAHYVELTGELDAPLLARAVVAGLAEADTLRMRFT-EDNGEVWQWVDPALTFPLPEI 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2243 ----EPTSPEDV-LAQLQAHTEpRTRRIDLSQAPLFRADIAHDPlqNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQA 2317
Cdd:PRK10252   88 idlrTQPDPHAAaQALMQADLQ-QDLRVDSGKPLVFHQLIQLGD--NRWYWYQRYHHLLVDGFSFPAITRRIAAIYCAWL 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2318 DALPTPLPyrNFIAQTLSVPN----SAHEAYFRD------KLADVDEPT--APfGLLNVQGSGGDIHeaRLVLDATlASA 2385
Cdd:PRK10252  165 RGEPTPAS--PFTPFADVVEEyqryRASEAWQRDaafwaeQRRQLPPPAslSP-APLPGRSASADIL--RLKLEFT-DGA 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2386 IRQQARHLGVSPgvLFHVAWAQV---LAQTSGRDDVVFGSVLLGRLAGAegADRIMGMFINTLPLRISLADRGA-AEVVE 2461
Cdd:PRK10252  239 FRQLAAQASGVQ--RPDLALALValwLGRLCGRMDYAAGFIFMRRLGSA--ALTATGPVLNVLPLRVHIAAQETlPELAT 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2462 RTSHDLMTLLEHEQAPLALAQRCSG-VAPPMPLFSTLLN-----YRHTqassTDNTLSDIRVLTSEERTNYPLTLAVDDR 2535
Cdd:PRK10252  315 RLAAQLKKMRRHQRYDAEQIVRDSGrAAGDEPLFGPVLNikvfdYQLD----FPGVQAQTHTLATGPVNDLELALFPDEH 390

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2536 GeGFSLV----AQTLEDIDPHRLLNYLMTAISSLVDAletePQRSILNLPVLPDSERQQmLVDFNATDADIPRHALIhEL 2611
Cdd:PRK10252  391 G-GLSIEilanPQRYDEATLIAHAERLKALIAQFAAD----PALLCGDVDILLPGEYAQ-LAQVNATAVEIPETTLS-AL 463

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2612 FEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYP 2691
Cdd:PRK10252  464 VAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYP 543

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2692 VERLRYMLDDAKPVALISQSAHLGIMNGslpvilLDDGETRPFDNePDTPLDARKQGLT-PRHLAYVIYTSGSTGKPKGV 2770
Cdd:PRK10252  544 DDRLKMMLEDARPSLLITTADQLPRFAD------VPDLTSLCYNA-PLAPQGAAPLQLSqPHHTAYIIFTSGSTGRPKGV 616

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2771 MVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQAT 2850
Cdd:PRK10252  617 MVGQTAIVNRLLWMQNHYPLTADDVVLQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFFAEYGVTTTHFV 696

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2851 PStwrML--------VELRDFALPPGFKALCGGEALPENLATALLQKVTT-LWNLYGPTE----TTIW-----STLNGLT 2912
Cdd:PRK10252  697 PS---MLaafvasltPEGARQSCASLRQVFCSGEALPADLCREWQQLTGApLHNLYGPTEaavdVSWYpafgeELAAVRG 773

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2913 TPTPyIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgAPEARMYKTGDLGRWLPD 2992
Cdd:PRK10252  774 SSVP-IGYPVWNTGLRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPF--APGERMYRTGDVARWLDD 850

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2993 GTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAR-----EDSPGD-KRLVAYLLAQPDTVLEPADLRQRLS 3066
Cdd:PRK10252  851 GAVEYLGRSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHACvinqaAATGGDaRQLVGYLVSQSGLPLDTSALQAQLR 930

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3067 EGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPD-QSAMATRgyeAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGG 3145
Cdd:PRK10252  931 ERLPPHMVPVVLLQLDQLPLSANGKLDRKALPLPElKAQVPGR---APKTGTETIIAAAFSSLLGCDVVDADADFFALGG 1007

                        1050      1060      1070      1080
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 3146 HSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILAAQIHAA 3191
Cdd:PRK10252 1008 HSLLAMKLAAQLSRQFARQVTPGQVMVASTVAKLATLLDAEEDESR 1053
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
 487-964 5.87e-176

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 548.78  E-value: 5.87e-176
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd17652     1 PDAPAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLTQsaqvaqlnstlptvlldtpaaaacpdtnpvvqglhAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd17652    81 ADARPALLLTT-----------------------------------PDNLAYVIYTSGSTGRPKGVVVTHRGLANLAAAQ 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 HTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLgs 725
Cdd:cd17652   126 IAAFDVGPGSRVLQFASPSFDASVWElLMALLAGATLVLAPAEELLPGEPLADLLREHRITHVTLPPAALAALPPDDL-- 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  726 dPAYQpaQVLIGGEAISPAV---WSRlqslsDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQP 802
Cdd:cd17652   204 -PDLR--TLVVAGEACPAELvdrWAP-----GRRMINAYGPTETTVCATMAGPLPGGGVPPIGRPVPGTRVYVLDARLRP 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  803 VPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSAdPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGE 882
Cdd:cd17652   276 VPPGVPGELYIAGAGLARGYLNRPGLTAERFVADPFGA-PGSRMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGE 354

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  883 IESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKA 962
Cdd:cd17652   355 VEAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRA 434


                  ..
gi 641744967  963 LP 964
Cdd:cd17652   435 LP 436
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
 2609-3097 2.60e-175

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 549.19  E-value: 2.60e-175
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2609 HELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDP 2688
Cdd:cd17646     1 HALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2689 TYPVERLRYMLDDAKPVALISQSAHLGIMNGSLPVILLDDGEtrpFDNEPDTPLDARKQgltPRHLAYVIYTSGSTGKPK 2768
Cdd:cd17646    81 GYPADRLAYMLADAGPAVVLTTADLAARLPAGGDVALLGDEA---LAAPPATPPLVPPR---PDNLAYVIYTSGSTGRPK 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2769 GVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQ 2848
Cdd:cd17646   155 GVMVTHAGIVNRLLWMQDEYPLGPGDRVLQKTPLSFDVSVWELFWPLVAGARLVVARPGGHRDPAYLAALIREHGVTTCH 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2849 ATPSTWRMLVELRDFALPPGFK-ALCGGEALPENLATALLQKV-TTLWNLYGPTETTIWSTL-----NGLTTPTPyIGHP 2921
Cdd:cd17646   235 FVPSMLRVFLAEPAAGSCASLRrVFCSGEALPPELAARFLALPgAELHNLYGPTEAAIDVTHwpvrgPAETPSVP-IGRP 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2922 IANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgAPEARMYKTGDLGRWLPDGTLEYLGRN 3001
Cdd:cd17646   314 VPNTRLYVLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAERFVPDPF--GPGSRMYRTGDLARWRPDGALEFLGRS 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3002 DFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPD-TVLEPADLRQRLSEGVAEYMIPSAFVT 3080
Cdd:cd17646   392 DDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGaAGPDTAALRAHLAERLPEYMVPAAFVV 471

                         490
                  ....*....|....*..
gi 641744967 3081 LDAFPLTPNGKLDRKAL 3097
Cdd:cd17646   472 LDALPLTANGKLDRAAL 488
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
 1555-1956 2.69e-174

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 542.63  E-value: 2.69e-174
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1555 TYDALNRRANQLAHHLIDL-GVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA 1633
Cdd:TIGR01733    1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1634 NQRALLTGDVPRILLDTADFSHLSEDNPHVPGLDAH----HLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTP 1709
Cdd:TIGR01733   81 ALASRLAGLVLPVILLDPLELAALDDAPAPPPPDAPsgpdDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGLDP 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1710 DDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKD-AAYLAQLIERTGITTLHFVPSMLQQFVQWADAdcACDSLR 1788
Cdd:TIGR01733  161 DDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDEERDdAALLAALIAEHPVTVLNLTPSLLALLAAALPP--ALASLR 238

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1789 RVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDDHRSF--VPIGRPIANTQLYILDTLGQPVPLGV 1865
Cdd:TIGR01733  239 LVILGGEALTPALVDRWRARGpGARLINLYGPTETTVWSTATLVDPDDAPREspVPIGRPLANTRLYVLDDDLRPVPVGV 318

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1866 AGELHIGGVGVARGYLNRPDLTAERFIPDPFINQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARL 1945
Cdd:TIGR01733  319 VGELYIGGPGVARGYLNRPELTAERFVPDPFAGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAAL 398

                          410
                   ....*....|.
gi 641744967  1946 MQCPGVQEAVV 1956
Cdd:TIGR01733  399 LRHPGVREAVV 409
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
 479-963 2.85e-174

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 546.10  E-value: 2.85e-174
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  479 FEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:cd17646     4 VAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYP 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNPVVqGLHAAHLAYVIYTSGSTGRPKGVMVAHRN 638
Cdd:cd17646    84 ADRLAYMLADAGPAVVLTTADLAARLPAGGDVALLGDEALAAPPATPPLV-PPRPDNLAYVIYTSGSTGRPKGVMVTHAG 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  639 VINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQW 717
Cdd:cd17646   163 IVNRLLWMQDEYPLGPGDRVLQKTPLSFDVSVWElFWPLVAGARLVVARPGGHRDPAYLAALIREHGVTTCHFVPSMLRV 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  718 LLD-AGLGSDPAYQpaQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPT--IGKPLANTRLY 794
Cdd:cd17646   243 FLAePAAGSCASLR--RVFCSGEALPPELAARFLALPGAELHNLYGPTEAAIDVTHWPVRGPAETPSvpIGRPVPNTRLY 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  795 ILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsaDPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVR 874
Cdd:cd17646   321 VLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAERFVPDPF--GPGSRMYRTGDLARWRPDGALEFLGRSDDQVKIR 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  875 GFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAE-LHPAALRQQLAASLADYMIPSAFVTLDALPLT 953
Cdd:cd17646   399 GFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGAAgPDTAALRAHLAERLPEYMVPAAFVVLDALPLT 478

                         490
                  ....*....|
gi 641744967  954 PNGKLDRKAL 963
Cdd:cd17646   479 ANGKLDRAAL 488
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
 2610-3097 1.17e-173

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 544.10  E-value: 1.17e-173
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2610 ELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPT 2689
Cdd:cd12117     1 ELFEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2690 YPVERLRYMLDDAKPVALISQSAHLGIMNGSLPVILLDDGETRPFDNEPDTPLDarkqgltPRHLAYVIYTSGSTGKPKG 2769
Cdd:cd12117    81 LPAERLAFMLADAGAKVLLTDRSLAGRAGGLEVAVVIDEALDAGPAGNPAVPVS-------PDDLAYVMYTSGSTGRPKG 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2770 VMVEHANMVNfLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQA 2849
Cdd:cd12117   154 VAVTHRGVVR-LVKNTNYVTLGPDDRVLQTSPLAFDASTFEIWGALLNGARLVLAPKGTLLDPDALGALIAEEGVTVLWL 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2850 TPSTWRMLVELRDFALPPGFKALCGGEALPENLATALLQKV--TTLWNLYGPTETTIWST--------LNGLTTPtpyIG 2919
Cdd:cd12117   233 TAALFNQLADEDPECFAGLRELLTGGEVVSPPHVRRVLAACpgLRLVNGYGPTENTTFTTshvvteldEVAGSIP---IG 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2920 HPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgAPEARMYKTGDLGRWLPDGTLEYLG 2999
Cdd:cd12117   310 RPIANTRVYVLDEDGRPVPPGVPGELYVGGDGLALGYLNRPALTAERFVADPF--GPGERLYRTGDLARWLPDGRLEFLG 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3000 RNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDtvLEPADLRQRLSEGVAEYMIPSAFV 3079
Cdd:cd12117   388 RIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDKRLVAYVVAEGA--LDAAELRAFLRERLPAYMVPAAFV 465

                         490
                  ....*....|....*...
gi 641744967 3080 TLDAFPLTPNGKLDRKAL 3097
Cdd:cd12117   466 VLDELPLTANGKVDRRAL 483
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
 2610-3101 6.33e-173

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 542.30  E-value: 6.33e-173
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2610 ELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPT 2689
Cdd:cd17655     1 ELFEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2690 YPVERLRYMLDDAKPVALISQSAHLGIMNGSLPVILLDDGETRpfdNEPDTPLDARKQgltPRHLAYVIYTSGSTGKPKG 2769
Cdd:cd17655    81 YPEERIQYILEDSGADILLTQSHLQPPIAFIGLIDLLDEDTIY---HEESENLEPVSK---SDDLAYVIYTSGSTGKPKG 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2770 VMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQA 2849
Cdd:cd17655   155 VMIEHRGVVNLVEWANKVIYQGEHLRVALFASISFDASVTEIFASLLSGNTLYIVRKETVLDGQALTQYIRQNRITIIDL 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2850 TPSTWRMLVELRDFALPPGFKALCGGEALPENLATALLQKVT---TLWNLYGPTETTIWSTLNGL-----TTPTPYIGHP 2921
Cdd:cd17655   235 TPAHLKLLDAADDSEGLSLKHLIVGGEALSTELAKKIIELFGtnpTITNAYGPTETTVDASIYQYepetdQQVSVPIGKP 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2922 IANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgAPEARMYKTGDLGRWLPDGTLEYLGRN 3001
Cdd:cd17655   315 LGNTRIYILDQYGRPQPVGVAGELYIGGEGVARGYLNRPELTAEKFVDDPF--VPGERMYRTGDLARWLPDGNIEFLGRI 392

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3002 DFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDtvLEPADLRQRLSEGVAEYMIPSAFVTL 3081
Cdd:cd17655   393 DHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKE--LPVAQLREFLARELPDYMIPSYFIKL 470

                         490       500
                  ....*....|....*....|
gi 641744967 3082 DAFPLTPNGKLDRKALPAPD 3101
Cdd:cd17655   471 DEIPLTPNGKVDRKALPEPD 490
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
30-1067 5.07e-171

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 566.60  E-value: 5.07e-171
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICwQGLHQPVQVVWRQAPLTVNTL 109
Cdd:PRK10252    9 PLVAAQPGIWMAEKLSPLPSAWSVAHYVELTGELDAPLLARAVVAGLAEADTLRMRFT-EDNGEVWQWVDPALTFPLPEI 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  110 tttssdtvpAQLRAATDPSNHRLNLSNAPLLSA--TTAHDP-------VCGE--WLLSLSIHHLISDHITQALIIDEI-- 176
Cdd:PRK10252   88 ---------IDLRTQPDPHAAAQALMQADLQQDlrVDSGKPlvfhqliQLGDnrWYWYQRYHHLLVDGFSFPAITRRIaa 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  177 --RLLLEDRPEALPKPLPYRNFIAQILSVPLSEHEQ----YFRNRLADIDTPTApfdlvdvqgngedITEARLSLDSSLA 250
Cdd:PRK10252  159 iyCAWLRGEPTPASPFTPFADVVEEYQRYRASEAWQrdaaFWAEQRRQLPPPAS-------------LSPAPLPGRSASA 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  251 DALRRQARHLGIS-SSVLFHVAWAQ----VLALT-------SGRDDVVFGSVLSGRLqGNlGADRVMGMFINTLPLRVSL 318
Cdd:PRK10252  226 DILRLKLEFTDGAfRQLAAQASGVQrpdlALALValwlgrlCGRMDYAAGFIFMRRL-GS-AALTATGPVLNVLPLRVHI 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  319 -RERSVHDVVQATSHELMMLLAH-----EQ----APLALAQQcsqvppplPLFSTLFN-----YRHSQKDASSQfwegMR 383
Cdd:PRK10252  304 aAQETLPELATRLAAQLKKMRRHqrydaEQivrdSGRAAGDE--------PLFGPVLNikvfdYQLDFPGVQAQ----TH 371

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  384 QLSgrertnypiTLSVDDLGDGFNLTAKTVMGVD----PERIVHYMLTA-IENLVTSLEKTPQQPAL---SQPILPKSER 455
Cdd:PRK10252  372 TLA---------TGPVNDLELALFPDEHGGLSIEilanPQRYDEATLIAhAERLKALIAQFAADPALlcgDVDILLPGEY 442

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  456 QQvLVDFNATDADFPREMLIQQrFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLE 535
Cdd:PRK10252  443 AQ-LAQVNATAVEIPETTLSAL-VAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVF 520

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  536 MMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNPVVQGlHAAH 615
Cdd:PRK10252  521 LTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTADQLPRFADVPDLTSLCYNAPLAPQGAAPLQLS-QPHH 599

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  616 LAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASV-KNWIQLLSGHTLVLV-PDALRaDA 693
Cdd:PRK10252  600 TAYIIFTSGSTGRPKGVMVGQTAIVNRLLWMQNHYPLTADDVVLQKTPCSFDVSVwEFFWPFIAGAKLVMAePEAHR-DP 678

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  694 HQLWRYFARHAVDLFDCTPVQLQWLLdAGLGSDPAYQP----AQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVD 769
Cdd:PRK10252  679 LAMQQFFAEYGVTTTHFVPSMLAAFV-ASLTPEGARQScaslRQVFCSGEALPADLCREWQQLTGAPLHNLYGPTEAAVD 757

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  770 AT---ACVVD----RTQPLPtIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSadP 842
Cdd:PRK10252  758 VSwypAFGEElaavRGSSVP-IGYPVWNTGLRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFA--P 834

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  843 AARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAR-----EDSPGD-TRLVAYLCA 916
Cdd:PRK10252  835 GERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHACvinqaAATGGDaRQLVGYLVS 914

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  917 RPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTA-FATRdyeAPQGGIETALAALWQELL 995
Cdd:PRK10252  915 QSGLPLDTSALQAQLRERLPPHMVPVVLLQLDQLPLSANGKLDRKALPLPELKAqVPGR---APKTGTETIIAAAFSSLL 991

                        1050      1060      1070      1080      1090      1100      1110
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967  996 GLDRVGRHDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAVTATAHDAPFT-----LPVADRTQP 1067
Cdd:PRK10252  992 GCDVVDADADFFALGGHSLLAMKLAAQLSRQfARQVTPGQVMVASTVAKLATLLDAEEDESRRLgfgtiLPLREGDGP 1069
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
 1530-2021 1.60e-169

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 530.74  E-value: 1.60e-169
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLD 1609
Cdd:cd12115     1 LHDLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1610 PGYPAERLAYMLDDASPVALLTqanqralltgdvprilldtadfshlsednphvpglDAHHLAYVIYTSGSTGKPKGVMN 1689
Cdd:cd12115    81 PAYPPERLRFILEDAQARLVLT-----------------------------------DPDDLAYVIYTSGSTGRPKGVAI 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRLVWMQNTYrlTPDDR--VLQKTPFSFDVSVWEFFWPLLYGARLVMArpdghKDAAYLAQLIERTGITTLHFV 1767
Cdd:cd12115   126 EHRNAAAFLQWAAAAF--SAEELagVLASTSICFDLSVFELFGPLATGGKVVLA-----DNVLALPDLPAAAEVTLINTV 198

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1768 PSMLQQFVQwadADCACDSLRRVICSGEALPAELQQRFFARFNA-QLHNLYGPTEAAIDVTFWACQPDDHRSfVPIGRPI 1846
Cdd:cd12115   199 PSAAAELLR---HDALPASVRVVNLAGEPLPRDLVQRLYARLQVeRVVNLYGPSEDTTYSTVAPVPPGASGE-VSIGRPL 274

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1847 ANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRND 1926
Cdd:cd12115   275 ANTQAYVLDRALQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDPF--GPGARLYRTGDLVRWRPDGLLEFLGRAD 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1927 FQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLD 2006
Cdd:cd12115   353 NQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPSRFVRLD 432

                         490
                  ....*....|....*
gi 641744967 2007 AFPLTPNGKLDRKAL 2021
Cdd:cd12115   433 ALPLTPNGKIDRSAL 447
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
 2612-3098 3.79e-168

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 528.45  E-value: 3.79e-168
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2612 FEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYP 2691
Cdd:cd17651     1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2692 VERLRYMLDDAKPVALISQSAHLGIMNGSLPVILLDDGETRPfdNEPDTPLDARkqgLTPRHLAYVIYTSGSTGKPKGVM 2771
Cdd:cd17651    81 AERLAFMLADAGPVLVLTHPALAGELAVELVAVTLLDQPGAA--AGADAEPDPA---LDADDLAYVIYTSGSTGRPKGVV 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2772 VEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATP 2851
Cdd:cd17651   156 MPHRSLANLVAWQARASSLGPGARTLQFAGLGFDVSVQEIFSTLCAGATLVLPPEEVRTDPPALAAWLDEQRISRVFLPT 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2852 STWRMLVEL--RDFALPPGFKAL-CGGEALPenlATALLQKVTT------LWNLYGPTETTIWS--TLNGLTTP---TPY 2917
Cdd:cd17651   236 VALRALAEHgrPLGVRLAALRYLlTGGEQLV---LTEDLREFCAglpglrLHNHYGPTETHVVTalSLPGDPAAwpaPPP 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSgaPEARMYKTGDLGRWLPDGTLEY 2997
Cdd:cd17651   313 IGRPIDNTRVYVLDAALRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPDPFV--PGARMYRTGDLARWLPDGELEF 390

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2998 LGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSA 3077
Cdd:cd17651   391 LGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRLVAYVVGDPEAPVDAAELRAALATHLPEYMVPSA 470

                         490       500
                  ....*....|....*....|.
gi 641744967 3078 FVTLDAFPLTPNGKLDRKALP 3098
Cdd:cd17651   471 FVLLDALPLTPNGKLDRRALP 491
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
 1542-2022 4.19e-168

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 526.55  E-value: 4.19e-168
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:cd17649     1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLTQANQralltgdvprilldtadfshlsednphvpgldahHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:cd17649    81 EDSGAGLLLTHHPR----------------------------------QLAYVIYTSGSTGTPKGVAVSHGPLAAHCQAT 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADAD 1781
Cdd:cd17649   127 AERYGLTPGDRELQFASFNFDGAHEQLLPPLICGACVVLRPDELWASADELAEMVRELGVTVLDLPPAYLQQLAEEADRT 206

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1782 CACD--SLRRVICSGEALPAELQQRFFARfNAQLHNLYGPTEAAIDVTFWACQPDDHR--SFVPIGRPIANTQLYILDTL 1857
Cdd:cd17649   207 GDGRppSLRLYIFGGEALSPELLRRWLKA-PVRLFNAYGPTEATVTPLVWKCEAGAARagASMPIGRPLGGRSAYILDAD 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1858 GQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIE 1937
Cdd:cd17649   286 LNPVPVGVTGELYIGGEGLARGYLGRPELTAERFVPDPF-GAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIE 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1938 LGEIEARLMQCPGVQEAVVVAReDSPGDTRLVAYLCPQPGVTP--DPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGK 2015
Cdd:cd17649   365 LGEIEAALLEHPGVREAAVVAL-DGAGGKQLVAYVVLRAAAAQpeLRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGK 443


                  ....*..
gi 641744967 2016 LDRKALP 2022
Cdd:cd17649   444 LDRKALP 450
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
 487-963 1.28e-166

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 522.25  E-value: 1.28e-166
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd17643     1 PEAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLTQsaqvaqlnstlptvlldtpaaaacpdtnpvvqglhAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd17643    81 ADSGPSLLLTD-----------------------------------PDDLAYVIYTSGSTGRPKGVVVSHANVLALFAAT 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 HTLLALDHPSRIALNASIVFDASV-KNWIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGS 725
Cdd:cd17643   126 QRWFGFNEDDVWTLFHSYAFDFSVwEIWGALLHGGRLVVVPYEVARSPEDFARLLRDEGVTVLNQTPSAFYQLVEAADRD 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  726 DPAyQPA--QVLIGGEAISPAV---WSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLP----TIGKPLANTRLYIL 796
Cdd:cd17643   206 GRD-PLAlrYVIFGGEALEAAMlrpWAGRFGLDRPQLVNMYGITETTVHVTFRPLDAADLPAaaasPIGRPLPGLRVYVL 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  797 DAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSAdPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGF 876
Cdd:cd17643   285 DADGRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVANPFGG-PGSRMYRTGDLARRLPDGELEYLGRADEQVKIRGF 363

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  877 RIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNG 956
Cdd:cd17643   364 RIELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAAADIAELRALLKELLPDYMVPARYVPLDALPLTVNG 443


                  ....*..
gi 641744967  957 KLDRKAL 963
Cdd:cd17643   444 KLDRAAL 450
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
 487-963 3.14e-166

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 522.24  E-value: 3.14e-166
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd12116     1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNPVvqGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd12116    81 EDAEPALVLTDDALPDRLPAGLPVLLLALAAAAAAPAAPRT--PVSPDDLAYVIYTSGSTGRPKGVVVSHRNLVNFLHSM 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 HTLLALDHPSRIALNASIVFDASV-KNWIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLgs 725
Cdd:cd12116   159 RERLGLGPGDRLLAVTTYAFDISLlELLLPLLAGARVVIAPRETQRDPEALARLIEAHSITVMQATPATWRMLLDAGW-- 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  726 dPAYQPAQVLIGGEAISPAVWSRLQSLSdTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPI 805
Cdd:cd12116   237 -QGRAGLTALCGGEALPPDLAARLLSRV-GSLWNLYGPTETTIWSTAARVTAAAGPIPIGRPLANTQVYVLDAALRPVPP 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  806 GVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsADPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIES 885
Cdd:cd12116   315 GVPGELYIGGDGVAQGYLGRPALTAERFVPDPF-AGPGSRLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEA 393

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967  886 RLLRCPGVQDAVVIAREDsPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd12116   394 ALAAHPGVAQAAVVVRED-GGDRRLVAYVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
 2633-3032 1.22e-165

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 517.59  E-value: 1.22e-165
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2633 SYDELNRRANRLAHHLIS-FGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQS 2711
Cdd:TIGR01733    1 TYRELDERANRLARHLRAaGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2712 AHLGIMNG-SLPVILLDDGETRPFDNEPDTPLDARKQGltPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGI 2790
Cdd:TIGR01733   81 ALASRLAGlVLPVILLDPLELAALDDAPAPPPPDAPSG--PDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGL 158

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2791 AQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAM-LIERHAVSFMQATPSTWRMLVELRDFALPpGF 2869
Cdd:TIGR01733  159 DPDDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDEERDDAALLAaLIAEHPVTVLNLTPSLLALLAAALPPALA-SL 237

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2870 KALC-GGEALPENLATALLQKV--TTLWNLYGPTETTIWST-------LNGLTTPTPyIGHPIANTQIYILDAQGRVVPL 2939
Cdd:TIGR01733  238 RLVIlGGEALTPALVDRWRARGpgARLINLYGPTETTVWSTatlvdpdDAPRESPVP-IGRPLANTRLYVLDDDLRPVPV 316

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2940 GVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIET 3019
Cdd:TIGR01733  317 GVVGELYIGGPGVARGYLNRPELTAERFVPDPFAGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEA 396

                          410
                   ....*....|...
gi 641744967  3020 RLARCHGVHDAVV 3032
Cdd:TIGR01733  397 ALLRHPGVREAVV 409
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
 2620-3098 1.36e-165

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 518.73  E-value: 1.36e-165
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd17652     1 PDAPAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQsahlgimngslpvillddgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVN 2779
Cdd:cd17652    81 ADARPALLLTT----------------------------------------PDNLAYVIYTSGSTGRPKGVVVTHRGLAN 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTwrmLVE 2859
Cdd:cd17652   121 LAAAQIAAFDVGPGSRVLQFASPSFDASVWELLMALLAGATLVLAPAEELLPGEPLADLLREHRITHVTLPPAA---LAA 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2860 LRDFALPPGFKALCGGEALPENLAtALLQKVTTLWNLYGPTETTIWSTLNGLTTP--TPYIGHPIANTQIYILDAQGRVV 2937
Cdd:cd17652   198 LPPDDLPDLRTLVVAGEACPAELV-DRWAPGRRMINAYGPTETTVCATMAGPLPGggVPPIGRPVPGTRVYVLDARLRPV 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2938 PLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEI 3017
Cdd:cd17652   277 PPGVPGELYIAGAGLARGYLNRPGLTAERFVADPF-GAPGSRMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEV 355

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3018 ETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd17652   356 EAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435


                  .
gi 641744967 3098 P 3098
Cdd:cd17652   436 P 436
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
 2608-3098 2.21e-165

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 519.68  E-value: 2.21e-165
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLD 2687
Cdd:cd17644     2 IHQLFEEQVERTPDAVAVVFEDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPLD 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2688 PTYPVERLRYMLDDAKPVALISQsahlgimngslpvillddgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGKP 2767
Cdd:cd17644    82 PNYPQERLTYILEDAQISVLLTQ----------------------------------------PENLAYVIYTSGSTGKP 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2768 KGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFM 2847
Cdd:cd17644   122 KGVMIEHQSLVNLSHGLIKEYGITSSDRVLQFASIAFDVAAEEIYVTLLSGATLVLRPEEMRSSLEDFVQYIQQWQLTVL 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2848 QATPSTWRMLVE---LRDFALPPGFK-ALCGGEA-LPENLATalLQKVT----TLWNLYGPTETTIWSTLNGLTTPT--- 2915
Cdd:cd17644   202 SLPPAYWHLLVLellLSTIDLPSSLRlVIVGGEAvQPELVRQ--WQKNVgnfiQLINVYGPTEATIAATVCRLTQLTern 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2916 ---PYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGAPEARMYKTGDLGRWLPD 2992
Cdd:cd17644   280 itsVPIGRPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELTAEKFISHPFNSSESERLYKTGDLARYLPD 359

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2993 GTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEY 3072
Cdd:cd17644   360 GNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYIVPHYEESPSTVELRQFLKAKLPDY 439

                         490       500
                  ....*....|....*....|....*.
gi 641744967 3073 MIPSAFVTLDAFPLTPNGKLDRKALP 3098
Cdd:cd17644   440 MIPSAFVVLEELPLTPNGKIDRRALP 465
PRK05691 PRK05691
peptide synthase; Validated
 2-1048 2.71e-163

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 571.34  E-value: 2.71e-163
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    2 PLVTLSQEEIDTViatvPDGVANVQDIYPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDI 81
Cdd:PRK05691 3235 PLAQLTQAQLDAL----PVPAAEIEDVYPLTPMQEGLLLHTLLEPGTGLYYMQDRYRINSALDPERFAQAWQAVVARHEA 3310

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   82 LRTAICWQGLHQPVQVVWRQAPLTVNTL--TTTSSDTVPAQLRAATDPSNHR-LNLSNAPLLSATTAHDPVCGEWLLsLS 158
Cdd:PRK05691 3311 LRASFSWNAGETMLQVIHKPGRTPIDYLdwRGLPEDGQEQRLQALHKQEREAgFDLLNQPPFHLRLIRVDEARYWFM-MS 3389

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  159 IHHLISDHITQALIIDEI----RLLLEDRPEALPKPLPYRNFIAQILSVPLSEHEQYFRNRLADIDTPTA-----PFdLV 229
Cdd:PRK05691 3390 NHHILIDAWCRSLLMNDFfeiyTALGEGREAQLPVPPRYRDYIGWLQRQDLAQARQWWQDNLRGFERPTPipsdrPF-LR 3468

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  230 DVQGN--GEDITEARLSLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGADRVMGM 307
Cdd:PRK05691 3469 EHAGDsgGMVVGDCYTRLDAADGARLRELAQAHQLTVNTFAQAAWALVLRRYSGDRDVLFGVTVAGRPVSMPQMQRTVGL 3548

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  308 FINTLPLRVSLRE----RSVHDVVQATSHELMMLLAHEQAPLALAQQCSQVPPPLPLFSTLFNY-----------RHSQK 372
Cdd:PRK05691 3549 FINSIALRVQLPAagqrCSVRQWLQGLLDSNMELREYEYLPLVAIQECSELPKGQPLFDSLFVFenapvevsvldRAQSL 3628

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  373 DASSQfwegmrqlSGRERTNYPITLSV---DDLG-----DGFNLTAKTVmgvdpERivhyMLTAIENLVTSLEKTPQQPA 444
Cdd:PRK05691 3629 NASSD--------SGRTHTNFPLTAVCypgDDLGlhlsyDQRYFDAPTV-----ER----LLGEFKRLLLALVQGFHGDL 3691

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  445 LSQPILPKSERQQVLVDFNATDADFPREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDD 524
Cdd:PRK05691 3692 SELPLLGEQERDFLLDGCNRSERDYPLEQSYVRLFEAQVAAHPQRIAASCLDQQWSYAELNRAANRLGHALRAAGVGVDQ 3771

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  525 RVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLNSTL--------PTVLL-DT 595
Cdd:PRK05691 3772 PVALLAERGLDLLGMIVGSFKAGAGYLPLDPGLPAQRLQRIIELSRTPVLVCSAACREQARALLdelgcanrPRLLVwEE 3851

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  596 PAAAACPDTNPvvqGLHAA--HLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVknW 673
Cdd:PRK05691 3852 VQAGEVASHNP---GIYSGpdNLAYVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQSFDISV--W 3926

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  674 IQL---LSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDaglgsdpayQPAQVLIG-------GEAISP 743
Cdd:PRK05691 3927 QFLaapLFGARVEIVPNAIAHDPQGLLAHVQAQGITVLESVPSLIQGMLA---------EDRQALDGlrwmlptGEAMPP 3997

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  744 AVWSR-LQSLSDTRFINVYGPTECTVDATACVVD----RTQPLPtIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGV 818
Cdd:PRK05691 3998 ELARQwLQRYPQIGLVNAYGPAECSDDVAFFRVDlastRGSYLP-IGSPTDNNRLYLLDEALELVPLGAVGELCVAGTGV 4076

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  819 ARGYLHRPDLTAERFIPDPFSAdPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVV 898
Cdd:PRK05691 4077 GRGYVGDPLRTALAFVPHPFGA-PGERLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAV 4155

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  899 IAREDSPGDtRLVAYLCARpDAELHPAAL----RQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAFATR 974
Cdd:PRK05691 4156 AVQEGVNGK-HLVGYLVPH-QTVLAQGALleriKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKALPALDIGQLQSQ 4233

                        1050      1060      1070      1080      1090      1100      1110
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 641744967  975 DYEAPQGGIETALAALWQELLGLDRVGRHDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAV 1048
Cdd:PRK05691 4234 AYLAPRNELEQTLATIWADVLKVERVGVHDNFFELGGHSLLATQIASRVQKAlQRNVPLRAMFECSTVEELAEYI 4308
LCL_NRPS-like cd19540
LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; ...
 1067-1494 5.10e-161

LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380463 [Multi-domain]  Cd Length: 433  Bit Score: 505.42  E-value: 5.10e-161
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1067 PLPLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQI-DPDTLGFS 1145
Cdd:cd19540     1 RIPLSFAQQRLWFLNRLDGPSA-AYNIPLALRLTGALDVDALRAALADVVARHESLRTVFPEDDGGPYQVVlPAAEARPD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1146 LSSHDLrklDEAARTTRVAELAeqeaRARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAA 1225
Cdd:cd19540    80 LTVVDV---TEDELAARLAEAA----RRGFDLTAELPLRARLFRLGPDEHVLVLVVHHIAADGWSMAPLARDLATAYAAR 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1226 LEGSEANLPPLPVQYADYAVWQRQWLQGETLNDLR-----DYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAG 1300
Cdd:cd19540   153 RAGRAPDWAPLPVQYADYALWQRELLGDEDDPDSLaarqlAYWRETLAGLPEELELPTDRPRPAVASYRGGTVEFTIDAE 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1301 QLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQ 1380
Cdd:cd19540   233 LHARLAALAREHGATLFMVLHAALAVLLSRLGAGDDIPIGTPVAGRGDEALDDLVGMFVNTLVLRTDVSGDPTFAELLAR 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1381 VRERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQALTLPDLTLSAVERPQHSTHFDLSLSLIETEN- 1459
Cdd:cd19540   313 VRETDLAAFAHQDVPFERLVEALNPPRSTARHPLFQVMLAFQNTAAATLELPGLTVEPVPVDTGVAKFDLSFTLTERRDa 392

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|
gi 641744967 1460 -----GLNGGLVYATDLFDRETILRVVGYVENILMAMADD 1494
Cdd:cd19540   393 dgapaGLTGELEYATDLFDRSTAERLADRFVRVLEAVVAD 432
PRK05691 PRK05691
peptide synthase; Validated
 2125-3183 1.08e-160

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 562.87  E-value: 1.08e-160
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2125 DSTAITPDLLPLVTLTQPEIDritdTVSGGASNIQDIYPLAPLQEGILFHHLLQEQGDTYLLRSmvafthRERLDA---- 2200
Cdd:PRK05691 3225 GAGGLTPSDFPLAQLTQAQLD----ALPVPAAEIEDVYPLTPMQEGLLLHTLLEPGTGLYYMQD------RYRINSaldp 3294

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2201 --FLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILPINHFEPTS-PEDVLAQ-LQA-HTEPRTRRIDLSQAPLFRA 2275
Cdd:PRK05691 3295 erFAQAWQAVVARHEALRASFSWNAGETMLQVIHKPGRTPIDYLDWRGlPEDGQEQrLQAlHKQEREAGFDLLNQPPFHL 3374

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2276 DIAHDPLQNEWLLaLSFHHLISDHMTLALIVGEI----RLLLQHQADALPTPLPYRNFIAQTLSVPNSAHEAYFRDKLAD 2351
Cdd:PRK05691 3375 RLIRVDEARYWFM-MSNHHILIDAWCRSLLMNDFfeiyTALGEGREAQLPVPPRYRDYIGWLQRQDLAQARQWWQDNLRG 3453

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2352 VDEPTA-----PFGLLNVQGSGGDIHEARLV-LDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLL 2425
Cdd:PRK05691 3454 FERPTPipsdrPFLREHAGDSGGMVVGDCYTrLDAADGARLRELAQAHQLTVNTFAQAAWALVLRRYSGDRDVLFGVTVA 3533

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2426 GRLAGAEGADRIMGMFINTLPLRISLADRGA-AEVVERTSHDL---MTLLEHEQAPLALAQRCSGVAPPMPLFSTLLNYR 2501
Cdd:PRK05691 3534 GRPVSMPQMQRTVGLFINSIALRVQLPAAGQrCSVRQWLQGLLdsnMELREYEYLPLVAIQECSELPKGQPLFDSLFVFE 3613

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2502 HT--QASSTD-----NTLSDirvlTSEERTNYPLTlAV----DDRGEGFSLVAQTLEDIDPHRLLNYLMTAISSLVDALE 2570
Cdd:PRK05691 3614 NApvEVSVLDraqslNASSD----SGRTHTNFPLT-AVcypgDDLGLHLSYDQRYFDAPTVERLLGEFKRLLLALVQGFH 3688

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2571 TEpqrsILNLPVLPDSERQQMLVDFNATDADIPRHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLIS 2650
Cdd:PRK05691 3689 GD----LSELPLLGEQERDFLLDGCNRSERDYPLEQSYVRLFEAQVAAHPQRIAASCLDQQWSYAELNRAANRLGHALRA 3764

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2651 FGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQSAHLgimngSLPVILLD--D 2728
Cdd:PRK05691 3765 AGVGVDQPVALLAERGLDLLGMIVGSFKAGAGYLPLDPGLPAQRLQRIIELSRTPVLVCSAACR-----EQARALLDelG 3839

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2729 GETRP----FDNEPDTPLDARKQGL--TPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSL 2802
Cdd:PRK05691 3840 CANRPrllvWEEVQAGEVASHNPGIysGPDNLAYVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQ 3919

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2803 SFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPS-TWRMLVELRDfALPPGFKALCGGEALPEN 2881
Cdd:PRK05691 3920 SFDISVWQFLAAPLFGARVEIVPNAIAHDPQGLLAHVQAQGITVLESVPSlIQGMLAEDRQ-ALDGLRWMLPTGEAMPPE 3998

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2882 LATALLQKVTT--LWNLYGPTETT---IWSTLNGLTTPTPY--IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVR 2954
Cdd:PRK05691 3999 LARQWLQRYPQigLVNAYGPAECSddvAFFRVDLASTRGSYlpIGSPTDNNRLYLLDEALELVPLGAVGELCVAGTGVGR 4078

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2955 GYLGRPDLTAERFITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIA 3034
Cdd:PRK05691 4079 GYVGDPLRTALAFVPHPF-GAPGERLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVAV 4157

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3035 REDSPGdKRLVAYLLAQpDTVLEPADL----RQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPDQSAMATRGY 3110
Cdd:PRK05691 4158 QEGVNG-KHLVGYLVPH-QTVLAQGALleriKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKALPALDIGQLQSQAY 4235

                        1050      1060      1070      1080      1090      1100      1110
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967 3111 EAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVI 3183
Cdd:PRK05691 4236 LAPRNELEQTLATIWADVLKVERVGVHDNFFELGGHSLLATQIASRVQKALQRNVPLRAMFECSTVEELAEYI 4308
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
 487-964 1.68e-159

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 501.90  E-value: 1.68e-159
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd17649     1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLTQsaqvaqlnstlptvlldtpaaaacpdtnpvvqglHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd17649    81 EDSGAGLLLTH----------------------------------HPRQLAYVIYTSGSTGTPKGVAVSHGPLAAHCQAT 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 HTLLALDHPSRIALNASIVFDASVKNWIQ-LLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQL-QWLLDAGLG 724
Cdd:cd17649   127 AERYGLTPGDRELQFASFNFDGAHEQLLPpLICGACVVLRPDELWASADELAEMVRELGVTVLDLPPAYLqQLAEEADRT 206

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  725 SDPAYQPAQVLI-GGEAISPAVWSRLQSlSDTRFINVYGPTECTVDATACVV----DRTQPLPTIGKPLANTRLYILDAQ 799
Cdd:cd17649   207 GDGRPPSLRLYIfGGEALSPELLRRWLK-APVRLFNAYGPTEATVTPLVWKCeagaARAGASMPIGRPLGGRSAYILDAD 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  800 DQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSAdPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIE 879
Cdd:cd17649   286 LNPVPVGVTGELYIGGEGLARGYLGRPELTAERFVPDPFGA-PGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIE 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  880 AGEIESRLLRCPGVQDAVVIAReDSPGDTRLVAYLCARPDAELH--PAALRQQLAASLADYMIPSAFVTLDALPLTPNGK 957
Cdd:cd17649   365 LGEIEAALLEHPGVREAAVVAL-DGAGGKQLVAYVVLRAAAAQPelRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGK 443


                  ....*..
gi 641744967  958 LDRKALP 964
Cdd:cd17649   444 LDRKALP 450
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
 2620-3097 6.75e-159

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 500.30  E-value: 6.75e-159
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd17643     1 PEAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQsahlgimngslpvillddgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVN 2779
Cdd:cd17643    81 ADSGPSLLLTD----------------------------------------PDDLAYVIYTSGSTGRPKGVVVSHANVLA 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVE 2859
Cdd:cd17643   121 LFAATQRWFGFNEDDVWTLFHSYAFDFSVWEIWGALLHGGRLVVVPYEVARSPEDFARLLRDEGVTVLNQTPSAFYQLVE 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2860 --LRDFALPPGFK-ALCGGEALPENLATALLQKV----TTLWNLYGPTETTIWSTLNGLTTPTPY------IGHPIANTQ 2926
Cdd:cd17643   201 aaDRDGRDPLALRyVIFGGEALEAAMLRPWAGRFgldrPQLVNMYGITETTVHVTFRPLDAADLPaaaaspIGRPLPGLR 280

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2927 IYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGAPEaRMYKTGDLGRWLPDGTLEYLGRNDFQVK 3006
Cdd:cd17643   281 VYVLDADGRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVANPFGGPGS-RMYRTGDLARRLPDGELEYLGRADEQVK 359

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3007 VRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPL 3086
Cdd:cd17643   360 IRGFRIELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAAADIAELRALLKELLPDYMVPARYVPLDALPL 439

                         490
                  ....*....|.
gi 641744967 3087 TPNGKLDRKAL 3097
Cdd:cd17643   440 TVNGKLDRAAL 450
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
 2608-3097 1.33e-158

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 499.15  E-value: 1.33e-158
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLD 2687
Cdd:cd12115     1 LHDLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2688 PTYPVERLRYMLDDAKPVALISQsahlgimngslpvillddgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGKP 2767
Cdd:cd12115    81 PAYPPERLRFILEDAQARLVLTD----------------------------------------PDDLAYVIYTSGSTGRP 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2768 KGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLamlieRHAVSFM 2847
Cdd:cd12115   121 KGVAIEHRNAAAFLQWAAAAFSAEELAGVLASTSICFDLSVFELFGPLATGGKVVLADNVLALPDLPA-----AAEVTLI 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2848 QATPSTWRMLVELRdfALPPGFKALC-GGEALPENLATALLQK--VTTLWNLYGPTETTIWSTLNGLTT---PTPYIGHP 2921
Cdd:cd12115   196 NTVPSAAAELLRHD--ALPASVRVVNlAGEPLPRDLVQRLYARlqVERVVNLYGPSEDTTYSTVAPVPPgasGEVSIGRP 273

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2922 IANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSgaPEARMYKTGDLGRWLPDGTLEYLGRN 3001
Cdd:cd12115   274 LANTQAYVLDRALQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDPFG--PGARLYRTGDLVRWRPDGLLEFLGRA 351

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3002 DFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTL 3081
Cdd:cd12115   352 DNQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPSRFVRL 431

                         490
                  ....*....|....*.
gi 641744967 3082 DAFPLTPNGKLDRKAL 3097
Cdd:cd12115   432 DALPLTPNGKIDRSAL 447
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
 500-898 1.67e-158

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 497.17  E-value: 1.67e-158
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   500 TYRELNRRANQLAHHLIAL-GVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:TIGR01733    1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   579 AQVAQLNSTLPTVLL----DTPAAAACPDTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDH 654
Cdd:TIGR01733   81 ALASRLAGLVLPVILldplELAALDDAPAPPPPDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGLDP 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   655 PSRIALNASIVFDASV-KNWIQLLSGHTLVLVPDALRADAHQLWRYF-ARHAVDLFDCTPVQLQWLLDAGLGSDPAYQpa 732
Cdd:TIGR01733  161 DDRVLQFASLSFDASVeEIFGALLAGATLVVPPEDEERDDAALLAALiAEHPVTVLNLTPSLLALLAAALPPALASLR-- 238

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   733 QVLIGGEAISPAVWSRLQSL-SDTRFINVYGPTECTVDATACVVD-----RTQPLPtIGKPLANTRLYILDAQDQPVPIG 806
Cdd:TIGR01733  239 LVILGGEALTPALVDRWRARgPGARLINLYGPTETTVWSTATLVDpddapRESPVP-IGRPLANTRLYVLDDDLRPVPVG 317

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   807 VTGELHIGGAGVARGYLHRPDLTAERFIPDPFSADPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESR 886
Cdd:TIGR01733  318 VVGELYIGGPGVARGYLNRPELTAERFVPDPFAGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAA 397

                          410
                   ....*....|..
gi 641744967   887 LLRCPGVQDAVV 898
Cdd:TIGR01733  398 LLRHPGVREAVV 409
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
 1541-2022 1.32e-152

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 483.51  E-value: 1.32e-152
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1541 TPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYM 1620
Cdd:cd17656     1 TPDAVAVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1621 LDDASPVALLTQANQRALLTGDVPRILLDTADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVW 1700
Cdd:cd17656    81 MLDSGVRVVLTQRHLKSKLSFNKSTILLEDPSISQEDTSNIDYI-NNSDDLLYIIYTSGTTGKPKGVQLEHKNMVNLLHF 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1701 MQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQ-FVQWAD 1779
Cdd:cd17656   160 EREKTNINFSDKVLQFATCSFDVCYQEIFSTLLSGGTLYIIREETKRDVEQLFDLVKRHNIEVVFLPVAFLKFiFSEREF 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1780 ADCACDSLRRVICSGEALP-AELQQRFFARFNAQLHNLYGPTEAAIdVTFWACQPDDH-RSFVPIGRPIANTQLYILDTL 1857
Cdd:cd17656   240 INRFPTCVKHIITAGEQLViTNEFKEMLHEHNVHLHNHYGPSETHV-VTTYTINPEAEiPELPPIGKPISNTWIYILDQE 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1858 GQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIE 1937
Cdd:cd17656   319 QQLQPQGIVGELYISGASVARGYLNRQELTAEKFFPDPF--DPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIE 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1938 LGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTpdPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:cd17656   397 LGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFVMEQELN--ISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVD 474


                  ....*
gi 641744967 2018 RKALP 2022
Cdd:cd17656   475 RKALP 479
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
 475-963 4.11e-151

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 477.58  E-value: 4.11e-151
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  475 IQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMD 554
Cdd:cd12115     1 LHDLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  555 PAYPAERLAYILDDAAPVALLTQSAqvaqlnstlptvlldtpaaaacpdtnpvvqglhaaHLAYVIYTSGSTGRPKGVMV 634
Cdd:cd12115    81 PAYPPERLRFILEDAQARLVLTDPD-----------------------------------DLAYVIYTSGSTGRPKGVAI 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  635 AHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQLLS-GHTLVLVPDALradahQLWRYFARHAVDLFDCTPV 713
Cdd:cd12115   126 EHRNAAAFLQWAAAAFSAEELAGVLASTSICFDLSVFELFGPLAtGGKVVLADNVL-----ALPDLPAAAEVTLINTVPS 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  714 QLQWLLDAglgsDPAYQPAQVL-IGGEAISPAVWSRLQS-LSDTRFINVYGPTECTVDATACVVDR-TQPLPTIGKPLAN 790
Cdd:cd12115   201 AAAELLRH----DALPASVRVVnLAGEPLPRDLVQRLYArLQVERVVNLYGPSEDTTYSTVAPVPPgASGEVSIGRPLAN 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  791 TRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsaDPAARIYKTGDLARWLPDGNIDYLGRNDFQ 870
Cdd:cd12115   277 TQAYVLDRALQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDPF--GPGARLYRTGDLVRWRPDGLLEFLGRADNQ 354

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  871 IKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDAL 950
Cdd:cd12115   355 VKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPSRFVRLDAL 434

                         490
                  ....*....|...
gi 641744967  951 PLTPNGKLDRKAL 963
Cdd:cd12115   435 PLTPNGKIDRSAL 447
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
 2620-3098 1.67e-150

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 476.09  E-value: 1.67e-150
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd17649     1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQsahlgimngslpvillddgetrpfdnepdtpldarkqglTPRHLAYVIYTSGSTGKPKGVMVEHANMVN 2779
Cdd:cd17649    81 EDSGAGLLLTH---------------------------------------HPRQLAYVIYTSGSTGTPKGVAVSHGPLAA 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVE 2859
Cdd:cd17649   122 HCQATAERYGLTPGDRELQFASFNFDGAHEQLLPPLICGACVVLRPDELWASADELAEMVRELGVTVLDLPPAYLQQLAE 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2860 LRDFALPPGFKAL----CGGEALPENLATALLQKVTTLWNLYGPTETTIWSTL------NGLTTPTPYIGHPIANTQIYI 2929
Cdd:cd17649   202 EADRTGDGRPPSLrlyiFGGEALSPELLRRWLKAPVRLFNAYGPTEATVTPLVwkceagAARAGASMPIGRPLGGRSAYI 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2930 LDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRG 3009
Cdd:cd17649   282 LDADLNPVPVGVTGELYIGGEGLARGYLGRPELTAERFVPDPF-GAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRG 360

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3010 FRIELGEIETRLARCHGVHDAVVIAReDSPGDKRLVAYLLAQPDTVLE--PADLRQRLSEGVAEYMIPSAFVTLDAFPLT 3087
Cdd:cd17649   361 FRIELGEIEAALLEHPGVREAAVVAL-DGAGGKQLVAYVVLRAAAAQPelRAQLRTALRASLPDYMVPAHLVFLARLPLT 439

                         490
                  ....*....|.
gi 641744967 3088 PNGKLDRKALP 3098
Cdd:cd17649   440 PNGKLDRKALP 450
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
 487-963 2.34e-150

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 475.42  E-value: 2.34e-150
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd17650     1 PDAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLTQsaqvaqlnstlptvlldtpaaaacPDtnpvvqglhaaHLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd17650    81 EDSGAKLLLTQ------------------------PE-----------DLAYVIYTSGTTGKPKGVMVEHRNVAHAAHAW 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 HTLLALDH-PSRIALNASIVFDASVKNWIQ-LLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLG 724
Cdd:cd17650   126 RREYELDSfPVRLLQMASFSFDVFAGDFARsLLNGGTLVICPDEVKLDPAALYDLILKSRITLMESTPALIRPVMAYVYR 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  725 SDPAYQPAQVLIGGEAISPAVWSRLQS---LSDTRFINVYGPTECTVDATACVVDRTqPLPT-----IGKPLANTRLYIL 796
Cdd:cd17650   206 NGLDLSAMRLLIVGSDGCKAQDFKTLAarfGQGMRIINSYGVTEATIDSTYYEEGRD-PLGDsanvpIGRPLPNTAMYVL 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  797 DAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSadPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGF 876
Cdd:cd17650   285 DERLQPQPVGVAGELYIGGAGVARGYLNRPELTAERFVENPFA--PGERMYRTGDLARWRADGNVELLGRVDHQVKIRGF 362

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  877 RIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCarPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNG 956
Cdd:cd17650   363 RIELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYVV--AAATLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNG 440


                  ....*..
gi 641744967  957 KLDRKAL 963
Cdd:cd17650   441 KVDRRAL 447
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
 1542-2021 6.96e-149

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 472.53  E-value: 6.96e-149
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:cd12114     1 PDATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLTQANQrALLTGDVPRILLDTADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:cd12114    81 ADAGARLVLTDGPD-AQLDVAVFDVLILDLDALAAPAPPPPVD-VAPDDLAYVIFTSGSTGTPKGVMISHRAALNTILDI 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWA-DA 1780
Cdd:cd12114   159 NRRFAVGPDDRVLALSSLSFDLSVYDIFGALSAGATLVLPDEARRRDPAHWAELIERHGVTLWNSVPALLEMLLDVLeAA 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1781 DCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQP-DDHRSFVPIGRPIANTQLYILDTLG 1858
Cdd:cd12114   239 QALLPSLRLVLLSGDWIPLDLPARLRALApDARLISLGGATEASIWSIYHPIDEvPPDWRSIPYGRPLANQRYRVLDPRG 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1859 QPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPfinqPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIEL 1938
Cdd:cd12114   319 RDCPDWVPGELWIGGRGVALGYLGDPELTAARFVTHP----DGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIEL 394

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1939 GEIEARLMQCPGVQEAVVVAReDSPGDTRLVAYLCPQPGVTPD-PADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:cd12114   395 GEIEAALQAHPGVARAVVVVL-GDPGGKRLAAFVVPDNDGTPIaPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVD 473


                  ....
gi 641744967 2018 RKAL 2021
Cdd:cd12114   474 RAAL 477
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
 1531-2022 3.22e-147

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 466.26  E-value: 3.22e-147
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDP 1610
Cdd:cd17645     1 HQLFEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1611 GYPAERLAYMLDDASPVALLTQANQralltgdvprilldtadfshlsednphvpgldahhLAYVIYTSGSTGKPKGVMNS 1690
Cdd:cd17645    81 DYPGERIAYMLADSSAKILLTNPDD-----------------------------------LAYVIYTSGSTGLPKGVMIE 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1691 HRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITtLHFVPSM 1770
Cdd:cd17645   126 HHNLVNLCEWHRPYFGVTPADKSLVYASFSFDASAWEIFPHLTAGAALHVVPSERRLDLDALNDYFNQEGIT-ISFLPTG 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1771 L-QQFVQWADAdcacdSLRRVICSGEALpaelqqRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHRsfVPIGRPIANT 1849
Cdd:cd17645   205 AaEQFMQLDNQ-----SLRVLLTGGDKL------KKIERKGYKLVNNYGPTENTVVATSFEIDKPYAN--IPIGKPIDNT 271

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1850 QLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFInqPGARLYKTGDLARWLPDGSLEYLGRNDFQV 1929
Cdd:cd17645   272 RVYILDEALQLQPIGVAGELCIAGEGLARGYLNRPELTAEKFIVHPFV--PGERMYRTGDLAKFLPDGNIEFLGRLDQQV 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1930 KLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVtpDPADLRQQLGQHLAEYMVPGAFVTLDAFP 2009
Cdd:cd17645   350 KIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAYVTAPEEI--PHEELREWLKNDLPDYMIPTYFVHLKALP 427

                         490
                  ....*....|...
gi 641744967 2010 LTPNGKLDRKALP 2022
Cdd:cd17645   428 LTANGKVDRKALP 440
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
 1542-2021 8.02e-146

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 462.71  E-value: 8.02e-146
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:cd17650     1 PDAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLTQanqralltgdvprilldtadfshlsednphvPGldahHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:cd17650    81 EDSGAKLLLTQ-------------------------------PE----DLAYVIYTSGTTGKPKGVMVEHRNVAHAAHAW 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDD-RVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSM---LQQFVQW 1777
Cdd:cd17650   126 RREYELDSFPvRLLQMASFSFDVFAGDFARSLLNGGTLVICPDEVKLDPAALYDLILKSRITLMESTPALirpVMAYVYR 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1778 ADADCacDSLRRVICSGEALPAELQQRFFARFNAQLH--NLYGPTEAAIDVTFW--ACQPDDHRSFVPIGRPIANTQLYI 1853
Cdd:cd17650   206 NGLDL--SAMRLLIVGSDGCKAQDFKTLAARFGQGMRiiNSYGVTEATIDSTYYeeGRDPLGDSANVPIGRPLPNTAMYV 283

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1854 LDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRG 1933
Cdd:cd17650   284 LDERLQPQPVGVAGELYIGGAGVARGYLNRPELTAERFVENPF--APGERMYRTGDLARWRADGNVELLGRVDHQVKIRG 361

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1934 FRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPgvTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPN 2013
Cdd:cd17650   362 FRIELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYVVAAA--TLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPN 439


                  ....*...
gi 641744967 2014 GKLDRKAL 2021
Cdd:cd17650   440 GKVDRRAL 447
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
 1530-2021 1.68e-142

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 454.31  E-value: 1.68e-142
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLD 1609
Cdd:cd05918     1 VHDLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1610 PGYPAERLAYMLDDASPVALLTqanqralltgdvprilldtadfshlsednphvpgLDAHHLAYVIYTSGSTGKPKGVMN 1689
Cdd:cd05918    81 PSHPLQRLQEILQDTGAKVVLT----------------------------------SSPSDAAYVIFTSGSTGKPKGVVI 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAayLAQLIERTGITTLHFVPS 1769
Cdd:cd05918   127 EHRALSTSALAHGRALGLTSESRVLQFASYTFDVSILEIFTTLAAGGCLCIPSEEDRLND--LAGFINRLRVTWAFLTPS 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1770 MLQQfVQWADadcaCDSLRRVICSGEALPAELQQRFFARfnAQLHNLYGPTEAAIDVTFWACQPDDHRSFvpIGRPIANT 1849
Cdd:cd05918   205 VARL-LDPED----VPSLRTLVLGGEALTQSDVDTWADR--VRLINAYGPAECTIAATVSPVVPSTDPRN--IGRPLGAT 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1850 qLYILD--TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDP-----FINQPGARLYKTGDLARWLPDGSLEYL 1922
Cdd:cd05918   276 -CWVVDpdNHDRLVPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPawlkqEGSGRGRRLYRTGDLVRYNPDGSLEYV 354

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1923 GRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVA---REDSPGDTRLVAYLCPQPGVT-----------------PDP 1982
Cdd:cd05918   355 GRKDTQVKIRGQRVELGEIEHHLRQSLPGAKEVVVEvvkPKDGSSSPQLVAFVVLDGSSSgsgdgdslflepsdefrALV 434

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967 1983 ADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05918   435 AELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRAL 473
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
 487-964 4.80e-142

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 451.85  E-value: 4.80e-142
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALG-VQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYI 565
Cdd:cd17648     1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAeIRPDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  566 LDDAAPVALLTQSAQvaqlnstlptvlldtpaaaacpdtnpvvqglhaahLAYVIYTSGSTGRPKGVMVAHRNVINLATG 645
Cdd:cd17648    81 LEDTGARVVITNSTD-----------------------------------LAYAIYTSGTTGKPKGVLVEHGSVVNLRTS 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  646 LHTLLALDHPS--RIALNASIVFDASVKNW-IQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQwLLDag 722
Cdd:cd17648   126 LSERYFGRDNGdeAVLFFSNYVFDFFVEQMtLALLNGQKLVVPPDEMRFDPDRFYAYINREKVTYLSGTPSVLQ-QYD-- 202

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  723 LGSDPAYQpaQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDAtacvVDRTQPLP-----TIGKPLANTRLYILD 797
Cdd:cd17648   203 LARLPHLK--RVDAAGEEFTAPVFEKLRSRFAGLIINAYGPTETTVTN----HKRFFPGDqrfdkSLGRPVRNTKCYVLN 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  798 AQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSADPA------ARIYKTGDLARWLPDGNIDYLGRNDFQI 871
Cdd:cd17648   277 DAMKRVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFQTEQErargrnARLYKTGDLVRWLPSGELEYLGRNDFQV 356

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  872 KVRGFRIEAGEIESRLLRCPGVQDAVVIARED-----SPGDTRLVAYLCarPDAELHPAA-LRQQLAASLADYMIPSAFV 945
Cdd:cd17648   357 KIRGQRIEPGEVEAALASYPGVRECAVVAKEDasqaqSRIQKYLVGYYL--PEPGHVPESdLLSFLRAKLPRYMVPARLV 434

                         490
                  ....*....|....*....
gi 641744967  946 TLDALPLTPNGKLDRKALP 964
Cdd:cd17648   435 RLEGIPVTINGKLDVRALP 453
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
 486-964 5.40e-141

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 450.00  E-value: 5.40e-141
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  486 TPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYI 565
Cdd:cd17656     1 TPDAVAVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  566 LDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNpVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATG 645
Cdd:cd17656    81 MLDSGVRVVLTQRHLKSKLSFNKSTILLEDPSISQEDTSN-IDYINNSDDLLYIIYTSGTTGKPKGVQLEHKNMVNLLHF 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  646 LHTLLALDHPSRIALNASIVFDASVKNWIQ-LLSGHTLVLVPDALRADAHQLWRYFARHAVDLFdCTPVQLQWLL--DAG 722
Cdd:cd17656   160 EREKTNINFSDKVLQFATCSFDVCYQEIFStLLSGGTLYIIREETKRDVEQLFDLVKRHNIEVV-FLPVAFLKFIfsERE 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  723 LGSDPAYQPAQVLIGGEAIspAVWSRLQSLSDTRFI---NVYGPTECTVdATACVVDRTQP---LPTIGKPLANTRLYIL 796
Cdd:cd17656   239 FINRFPTCVKHIITAGEQL--VITNEFKEMLHEHNVhlhNHYGPSETHV-VTTYTINPEAEipeLPPIGKPISNTWIYIL 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  797 DAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsaDPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGF 876
Cdd:cd17656   316 DQEQQLQPQGIVGELYISGASVARGYLNRQELTAEKFFPDPF--DPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGY 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  877 RIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCarPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNG 956
Cdd:cd17656   394 RIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFV--MEQELNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNG 471


                  ....*...
gi 641744967  957 KLDRKALP 964
Cdd:cd17656   472 KVDRKALP 479
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
 1542-2022 5.24e-140

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 446.08  E-value: 5.24e-140
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLG-VKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYM 1620
Cdd:cd17648     1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAeIRPDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1621 LDDaspvallTQAnqRALLTGdvprilldtadfshlSEDnphvpgldahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVW 1700
Cdd:cd17648    81 LED-------TGA--RVVITN---------------STD-----------LAYAIYTSGTTGKPKGVLVEHGSVVNLRTS 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1701 MQNTY--RLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFvqwa 1778
Cdd:cd17648   126 LSERYfgRDNGDEAVLFFSNYVFDFFVEQMTLALLNGQKLVVPPDEMRFDPDRFYAYINREKVTYLSGTPSVLQQY---- 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1779 daDCA-CDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAID--VTFWacqPDDHRSFVPIGRPIANTQLYILD 1855
Cdd:cd17648   202 --DLArLPHLKRVDAAGEEFTAPVFEKLRSRFAGLIINAYGPTETTVTnhKRFF---PGDQRFDKSLGRPVRNTKCYVLN 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1856 TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFIN----QPG--ARLYKTGDLARWLPDGSLEYLGRNDFQV 1929
Cdd:cd17648   277 DAMKRVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFQTeqerARGrnARLYKTGDLVRWLPSGELEYLGRNDFQV 356

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1930 KLRGFRIELGEIEARLMQCPGVQEAVVVARED-----SPGDTRLVAYLCPQPGVTPDpADLRQQLGQHLAEYMVPGAFVT 2004
Cdd:cd17648   357 KIRGQRIEPGEVEAALASYPGVRECAVVAKEDasqaqSRIQKYLVGYYLPEPGHVPE-SDLLSFLRAKLPRYMVPARLVR 435

                         490
                  ....*....|....*...
gi 641744967 2005 LDAFPLTPNGKLDRKALP 2022
Cdd:cd17648   436 LEGIPVTINGKLDVRALP 453
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
 476-964 3.71e-139

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 443.15  E-value: 3.71e-139
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  476 QQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDP 555
Cdd:cd17645     1 HQLFEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  556 AYPAERLAYILDDAAPVALLTQSAQvaqlnstlptvlldtpaaaacpdtnpvvqglhaahLAYVIYTSGSTGRPKGVMVA 635
Cdd:cd17645    81 DYPGERIAYMLADSSAKILLTNPDD-----------------------------------LAYVIYTSGSTGLPKGVMIE 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  636 HRNVINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDL-FDCTPV 713
Cdd:cd17645   126 HHNLVNLCEWHRPYFGVTPADKSLVYASFSFDASAWEiFPHLTAGAALHVVPSERRLDLDALNDYFNQEGITIsFLPTGA 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  714 QLQWL-LDAglgsdpayQPAQVLI-GGEAISPAVWSRLQslsdtrFINVYGPTECTVDATACVVDRTQPLPTIGKPLANT 791
Cdd:cd17645   206 AEQFMqLDN--------QSLRVLLtGGDKLKKIERKGYK------LVNNYGPTENTVVATSFEIDKPYANIPIGKPIDNT 271

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  792 RLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSadPAARIYKTGDLARWLPDGNIDYLGRNDFQI 871
Cdd:cd17645   272 RVYILDEALQLQPIGVAGELCIAGEGLARGYLNRPELTAEKFIVHPFV--PGERMYRTGDLAKFLPDGNIEFLGRLDQQV 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  872 KVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAElhPAALRQQLAASLADYMIPSAFVTLDALP 951
Cdd:cd17645   350 KIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAYVTAPEEIP--HEELREWLKNDLPDYMIPTYFVHLKALP 427

                         490
                  ....*....|...
gi 641744967  952 LTPNGKLDRKALP 964
Cdd:cd17645   428 LTANGKVDRKALP 440
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
 2619-3098 2.10e-138

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 442.68  E-value: 2.10e-138
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:cd17656     1 TPDAVAVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISQsAHLGIM---NGSLpVILLDDGETRPFDNEPDTPLDArkqgltpRHLAYVIYTSGSTGKPKGVMVEHA 2775
Cdd:cd17656    81 MLDSGVRVVLTQ-RHLKSKlsfNKST-ILLEDPSISQEDTSNIDYINNS-------DDLLYIIYTSGTTGKPKGVQLEHK 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2776 NMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWR 2855
Cdd:cd17656   152 NMVNLLHFEREKTNINFSDKVLQFATCSFDVCYQEIFSTLLSGGTLYIIREETKRDVEQLFDLVKRHNIEVVFLPVAFLK 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2856 MLVELRDFA--LPPGFK-ALCGGEALPEN--LATALLQKVTTLWNLYGPTETTIWSTLN---GLTTPT-PYIGHPIANTQ 2926
Cdd:cd17656   232 FIFSEREFInrFPTCVKhIITAGEQLVITneFKEMLHEHNVHLHNHYGPSETHVVTTYTinpEAEIPElPPIGKPISNTW 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2927 IYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSgaPEARMYKTGDLGRWLPDGTLEYLGRNDFQVK 3006
Cdd:cd17656   312 IYILDQEQQLQPQGIVGELYISGASVARGYLNRQELTAEKFFPDPFD--PNERMYRTGDLARYLPDGNIEFLGRADHQVK 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3007 VRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLaqPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPL 3086
Cdd:cd17656   390 IRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFV--MEQELNISQLREYLAKQLPEYMIPSFFVPLDQLPL 467

                         490
                  ....*....|..
gi 641744967 3087 TPNGKLDRKALP 3098
Cdd:cd17656   468 TPNGKVDRKALP 479
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
 487-963 2.87e-136

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 436.32  E-value: 2.87e-136
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd12114     1 PDATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNPVVQGLHAahLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd12114    81 ADAGARLVLTDGPDAQLDVAVFDVLILDLDALAAPAPPPPVDVAPDD--LAYVIFTSGSTGTPKGVMISHRAALNTILDI 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 HTLLALDHPSRIALNASIVFDASVKNWIQLLS-GHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGS 725
Cdd:cd12114   159 NRRFAVGPDDRVLALSSLSFDLSVYDIFGALSaGATLVLPDEARRRDPAHWAELIERHGVTLWNSVPALLEMLLDVLEAA 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  726 DPAYQP-AQVLIGGEAISPAVWSRLQSL-SDTRFINVYGPTECTVDATACVVDRTQP-LPTI--GKPLANTRLYILDAQD 800
Cdd:cd12114   239 QALLPSlRLVLLSGDWIPLDLPARLRALaPDARLISLGGATEASIWSIYHPIDEVPPdWRSIpyGRPLANQRYRVLDPRG 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  801 QPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPfsadPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEA 880
Cdd:cd12114   319 RDCPDWVPGELWIGGRGVALGYLGDPELTAARFVTHP----DGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIEL 394

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  881 GEIESRLLRCPGVQDAVVIAReDSPGDTRLVAYLCARPDA-ELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLD 959
Cdd:cd12114   395 GEIEAALQAHPGVARAVVVVL-GDPGGKRLAAFVVPDNDGtPIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVD 473


                  ....
gi 641744967  960 RKAL 963
Cdd:cd12114   474 RAAL 477
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
 2620-3097 1.33e-134

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 430.35  E-value: 1.33e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd17650     1 PDAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAkpvalisqsahlgimngSLPVILLDdgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVN 2779
Cdd:cd17650    81 EDS-----------------GAKLLLTQ-----------------------PEDLAYVIYTSGTTGKPKGVMVEHRNVAH 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRKEPGIAQEDV-LLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLV 2858
Cdd:cd17650   121 AAHAWRREYELDSFPVrLLQMASFSFDVFAGDFARSLLNGGTLVICPDEVKLDPAALYDLILKSRITLMESTPALIRPVM 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2859 EL--RDFALPPGFKALCGGEAL-----PENLATALLQKvTTLWNLYGPTETTIWSTL---------NGLTTPtpyIGHPI 2922
Cdd:cd17650   201 AYvyRNGLDLSAMRLLIVGSDGckaqdFKTLAARFGQG-MRIINSYGVTEATIDSTYyeegrdplgDSANVP---IGRPL 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2923 ANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgAPEARMYKTGDLGRWLPDGTLEYLGRND 3002
Cdd:cd17650   277 PNTAMYVLDERLQPQPVGVAGELYIGGAGVARGYLNRPELTAERFVENPF--APGERMYRTGDLARWRADGNVELLGRVD 354

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3003 FQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLaqPDTVLEPADLRQRLSEGVAEYMIPSAFVTLD 3082
Cdd:cd17650   355 HQVKIRGFRIELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYVV--AAATLNTAELRAFLAKELPSYMIPSYYVQLD 432

                         490
                  ....*....|....*
gi 641744967 3083 AFPLTPNGKLDRKAL 3097
Cdd:cd17650   433 ALPLTPNGKVDRRAL 447
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
 1530-2023 1.91e-133

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 426.92  E-value: 1.91e-133
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLD 1609
Cdd:COG0318     1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1610 PGYPAERLAYMLDDASPVALLTqanqralltgdvprilldtadfshlsednphvpgldahhlAYVIYTSGSTGKPKGVMN 1689
Cdd:COG0318    81 PRLTAEELAYILEDSGARALVT----------------------------------------ALILYTSGTTGRPKGVML 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVS-VWEFFWPLLYGARLVMARpdgHKDAAYLAQLIERTGITTLHFVP 1768
Cdd:COG0318   121 THRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGlTVGLLAPLLAGATLVLLP---RFDPERVLELIERERVTVLFGVP 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1769 SMLQQFVQWADADCAC-DSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTfwaCQPDDH--RSFVPIGRP 1845
Cdd:COG0318   198 TMLARLLRHPEFARYDlSSLRLVVSGGAPLPPELLERFEERFGVRIVEGYGLTETSPVVT---VNPEDPgeRRPGSVGRP 274

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1846 IANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFiPDPFinqpgarlYKTGDLARWLPDGSLEYLGRN 1925
Cdd:COG0318   275 LPGVEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWNDPEATAEAF-RDGW--------LRTGDLGRLDEDGYLYIVGRK 345

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1926 DFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTL 2005
Cdd:COG0318   346 KDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAELDAEELRAFLRERLARYKVPRRVEFV 425

                         490
                  ....*....|....*...
gi 641744967 2006 DAFPLTPNGKLDRKALPA 2023
Cdd:COG0318   426 DELPRTASGKIDRRALRE 443
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
 2620-3098 3.44e-133

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 426.43  E-value: 3.44e-133
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFG-VRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:cd17648     1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAeIRPDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISqsahlgimngslpvillddgetrpfdnepdtpldarkqglTPRHLAYVIYTSGSTGKPKGVMVEHANMV 2778
Cdd:cd17648    81 LEDTGARVVIT----------------------------------------NSTDLAYAIYTSGTTGKPKGVLVEHGSVV 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2779 NFLCSMRKEPGIAQED--VLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPStwrm 2856
Cdd:cd17648   121 NLRTSLSERYFGRDNGdeAVLFFSNYVFDFFVEQMTLALLNGQKLVVPPDEMRFDPDRFYAYINREKVTYLSGTPS---- 196

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2857 LVELRDFALPPGFK-ALCGGEALPENLATALLQKVTTL-WNLYGPTETTIWSTLNGLTTPTPY---IGHPIANTQIYILD 2931
Cdd:cd17648   197 VLQQYDLARLPHLKrVDAAGEEFTAPVFEKLRSRFAGLiINAYGPTETTVTNHKRFFPGDQRFdksLGRPVRNTKCYVLN 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2932 AQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGAPE------ARMYKTGDLGRWLPDGTLEYLGRNDFQV 3005
Cdd:cd17648   277 DAMKRVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFQTEQErargrnARLYKTGDLVRWLPSGELEYLGRNDFQV 356

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3006 KVRGFRIELGEIETRLARCHGVHDAVVIARED-----SPGDKRLVAYLLAQPDTVLEpADLRQRLSEGVAEYMIPSAFVT 3080
Cdd:cd17648   357 KIRGQRIEPGEVEAALASYPGVRECAVVAKEDasqaqSRIQKYLVGYYLPEPGHVPE-SDLLSFLRAKLPRYMVPARLVR 435

                         490
                  ....*....|....*...
gi 641744967 3081 LDAFPLTPNGKLDRKALP 3098
Cdd:cd17648   436 LEGIPVTINGKLDVRALP 453
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
 1538-2021 6.14e-133

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 425.51  E-value: 6.14e-133
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:cd05945     1 AAANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLTqanqralltgdvprilldtadfshlsednphvpglDAHHLAYVIYTSGSTGKPKGVMNSHRALCNR 1697
Cdd:cd05945    81 REILDAAKPALLIA-----------------------------------DGDDNAYIIFTSGSTGRPKGVQISHDNLVSF 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1698 LVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQ-FVQ 1776
Cdd:cd05945   126 TNWMLSDFPLGPGDVFLNQAPFSFDLSVMDLYPALASGATLVPVPRDATADPKQLFRFLAEHGITVWVSTPSFAAMcLLS 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1777 WADADCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPDDHRSF--VPIGRPIANTQLYI 1853
Cdd:cd05945   206 PTFTPESLPSLRHFLFCGEVLPHKTARALQQRFpDARIYNTYGPTEATVAVTYIEVTPEVLDGYdrLPIGYAKPGAKLVI 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1854 LDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPfinqpGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRG 1933
Cdd:cd05945   286 LDEDGRPVPPGEKGELVISGPSVSKGYLNNPEKTAAAFFPDE-----GQRAYRTGDLVRLEADGLLFYRGRLDFQVKLNG 360

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1934 FRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVT-PDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTP 2012
Cdd:cd05945   361 YRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPGAEaGLTKAIKAELAERLPPYMIPRRFVYLDELPLNA 440


                  ....*....
gi 641744967 2013 NGKLDRKAL 2021
Cdd:cd05945   441 NGKIDRKAL 449
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
 2608-3097 1.60e-131

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 422.72  E-value: 1.60e-131
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLD 2687
Cdd:cd05918     1 VHDLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2688 PTYPVERLRYMLDDAK-PVALISQsahlgimngslpvillddgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGK 2766
Cdd:cd05918    81 PSHPLQRLQEILQDTGaKVVLTSS----------------------------------------PSDAAYVIFTSGSTGK 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2767 PKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLamLIERHAVSF 2846
Cdd:cd05918   121 PKGVVIEHRALSTSALAHGRALGLTSESRVLQFASYTFDVSILEIFTTLAAGGCLCIPSEEDRLNDLAG--FINRLRVTW 198

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2847 MQATPSTWRMLvelrDFALPPGFKALC-GGEALPENLATALLQKVTtLWNLYGPTETTIWSTLN--GLTTPTPYIGHPIA 2923
Cdd:cd05918   199 AFLTPSVARLL----DPEDVPSLRTLVlGGEALTQSDVDTWADRVR-LINAYGPAECTIAATVSpvVPSTDPRNIGRPLG 273

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2924 NTqIYILDAQ--GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDP-----FSGAPEARMYKTGDLGRWLPDGTLE 2996
Cdd:cd05918   274 AT-CWVVDPDnhDRLVPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPawlkqEGSGRGRRLYRTGDLVRYNPDGSLE 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2997 YLGRNDFQVKVRGFRIELGEIETRLARC-HGVHDAVV--IAREDSPGDKRLVAYLLAQ-----------------PDTVL 3056
Cdd:cd05918   353 YVGRKDTQVKIRGQRVELGEIEHHLRQSlPGAKEVVVevVKPKDGSSSPQLVAFVVLDgsssgsgdgdslflepsDEFRA 432

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 641744967 3057 EPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05918   433 LVAELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRAL 473
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
 1532-2021 1.96e-131

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 420.56  E-value: 1.96e-131
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1532 QLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:cd17653     1 DAFERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 YPAERLAYMLDDASPVALLTQANQralltgdvprilldtadfshlsednphvpgldaHHLAYVIYTSGSTGKPKGVMNSH 1691
Cdd:cd17653    81 LPSARIQAILRTSGATLLLTTDSP---------------------------------DDLAYIIFTSGSTGIPKGVMVPH 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1692 RALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDghkdaAYLAQLIerTGITTLHFVPSML 1771
Cdd:cd17653   128 RGVLNYVSQPPARLDVGPGSRVAQVLSIAFDACIGEIFSTLCNGGTLVLADPS-----DPFAHVA--RTVDALMSTPSIL 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1772 QQFvqwADADcaCDSLRRVICSGEALPAELQQRFfaRFNAQLHNLYGPTEAAIDVTFWACQPDDhrsFVPIGRPIANTQL 1851
Cdd:cd17653   201 STL---SPQD--FPNLKTIFLGGEAVPPSLLDRW--SPGRRLYNAYGPTECTISSTMTELLPGQ---PVTIGKPIPNSTC 270

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1852 YILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKL 1931
Cdd:cd17653   271 YILDADLQPVPEGVVGEICISGVQVARGYLGNPALTASKFVPDPF--WPGSRMYRTGDYGRWTEDGGLEFLGREDNQVKV 348

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1932 RGFRIELGEIEARLMQ-CPGVQEAVVVAREDspgdtRLVAYLCPQpgvTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPL 2010
Cdd:cd17653   349 RGFRINLEEIEEVVLQsQPEVTQAAAIVVNG-----RLVAFVTPE---TVDVDGLRSELAKHLPSYAVPDRIIALDSFPL 420

                         490
                  ....*....|.
gi 641744967 2011 TPNGKLDRKAL 2021
Cdd:cd17653   421 TANGKVDRKAL 431
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
 475-963 2.24e-131

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 422.34  E-value: 2.24e-131
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  475 IQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMD 554
Cdd:cd05918     1 VHDLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  555 PAYPAERLAYILDDA-APVALltqsaqvaqlnstlptvlldtpaaAACPDtnpvvqglhaaHLAYVIYTSGSTGRPKGVM 633
Cdd:cd05918    81 PSHPLQRLQEILQDTgAKVVL------------------------TSSPS-----------DAAYVIFTSGSTGKPKGVV 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  634 VAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKN-WIQLLSGHTLVLVPDALRADAhqLWRYFARHAVDLFDCTP 712
Cdd:cd05918   126 IEHRALSTSALAHGRALGLTSESRVLQFASYTFDVSILEiFTTLAAGGCLCIPSEEDRLND--LAGFINRLRVTWAFLTP 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  713 -VqlqwlldAGLgsdpaYQPAQV------LIGGEAISPAV---WSrlqslSDTRFINVYGPTECTVDATACVVDRTQPLP 782
Cdd:cd05918   204 sV-------ARL-----LDPEDVpslrtlVLGGEALTQSDvdtWA-----DRVRLINAYGPAECTIAATVSPVVPSTDPR 266

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  783 TIGKPLAnTRLYILDAQD--QPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDP-----FSADPAARIYKTGDLARW 855
Cdd:cd05918   267 NIGRPLG-ATCWVVDPDNhdRLVPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPawlkqEGSGRGRRLYRTGDLVRY 345

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  856 LPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVV---IAREDSPGDTRLVAYLCARPDAELHP-------- 924
Cdd:cd05918   346 NPDGSLEYVGRKDTQVKIRGQRVELGEIEHHLRQSLPGAKEVVvevVKPKDGSSSPQLVAFVVLDGSSSGSGdgdslfle 425

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 641744967  925 ---------AALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05918   426 psdefralvAELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRAL 473
LCL_NRPS cd19538
LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; ...
 1068-1477 2.01e-130

LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380461 [Multi-domain]  Cd Length: 432  Bit Score: 417.44  E-value: 2.01e-130
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1068 LPLSFSQQRLWFLAQLDpAASQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDtlGFSLS 1147
Cdd:cd19538     2 IPLSFAQRRLWFLHQLE-GPSATYNIPLVIKLKGKLDVQALQQALYDVVERHESLRTVFPEEDGVPYQLILEE--DEATP 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1148 SHDLRKLDEAArttrVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALE 1227
Cdd:cd19538    79 KLEIKEVDEEE----LESEINEAVRYPFDLSEEPPFRATLFELGENEHVLLLLLHHIAADGWSLAPLTRDLSKAYRARCK 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1228 GSEANLPPLPVQYADYAVWQRQWLQGETLNDLR-----DYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQL 1302
Cdd:cd19538   155 GEAPELAPLPVQYADYALWQQELLGDESDPDSLiarqlAYWKKQLAGLPDEIELPTDYPRPAESSYEGGTLTFEIDSELH 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1303 RRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVR 1382
Cdd:cd19538   235 QQLLQLAKDNNVTLFMVLQAGFAALLTRLGAGTDIPIGSPVAGRNDDSLEDLVGFFVNTLVLRTDTSGNPSFRELLERVK 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1383 ERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQALTLPDLTLSAVERPQHSTHFDLSLSLIE-----T 1457
Cdd:cd19538   315 ETNLEAYEHQDIPFERLVEALNPTRSRSRHPLFQIMLALQNTPQPSLDLPGLEAKLELRTVGSAKFDLTFELREqyndgT 394

                         410       420
                  ....*....|....*....|
gi 641744967 1458 ENGLNGGLVYATDLFDRETI 1477
Cdd:cd19538   395 PNGIEGFIEYRTDLFDHETI 414
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
 2609-3098 9.16e-130

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 416.19  E-value: 9.16e-130
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2609 HELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDP 2688
Cdd:cd17645     1 HQLFEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2689 TYPVERLRYMLDDAKPVALISQsahlgimngslpvillddgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGKPK 2768
Cdd:cd17645    81 DYPGERIAYMLADSSAKILLTN----------------------------------------PDDLAYVIYTSGSTGLPK 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2769 GVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVS--- 2845
Cdd:cd17645   121 GVMIEHHNLVNLCEWHRPYFGVTPADKSLVYASFSFDASAWEIFPHLTAGAALHVVPSERRLDLDALNDYFNQEGITisf 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2846 --------FMQATPSTWRMLvelrdfalppgfkaLCGGEALpenlaTALLQKVTTLWNLYGPTETTIWSTLNGLTTPTPY 2917
Cdd:cd17645   201 lptgaaeqFMQLDNQSLRVL--------------LTGGDKL-----KKIERKGYKLVNNYGPTENTVVATSFEIDKPYAN 261

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 --IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgAPEARMYKTGDLGRWLPDGTL 2995
Cdd:cd17645   262 ipIGKPIDNTRVYILDEALQLQPIGVAGELCIAGEGLARGYLNRPELTAEKFIVHPF--VPGERMYRTGDLAKFLPDGNI 339

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2996 EYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLaqPDTVLEPADLRQRLSEGVAEYMIP 3075
Cdd:cd17645   340 EFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAYVT--APEEIPHEELREWLKNDLPDYMIP 417

                         490       500
                  ....*....|....*....|...
gi 641744967 3076 SAFVTLDAFPLTPNGKLDRKALP 3098
Cdd:cd17645   418 TYFVHLKALPLTANGKVDRKALP 440
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
 2620-3097 2.12e-128

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 413.59  E-value: 2.12e-128
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd12114     1 PDATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAkpvalisqSAHLGIMNGSLPVILLDDGETRPFDNEPDTPLDAR-KQGLTPRHLAYVIYTSGSTGKPKGVMVEHANMV 2778
Cdd:cd12114    81 ADA--------GARLVLTDGPDAQLDVAVFDVLILDLDALAAPAPPpPVDVAPDDLAYVIFTSGSTGTPKGVMISHRAAL 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2779 NFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLV 2858
Cdd:cd12114   153 NTILDINRRFAVGPDDRVLALSSLSFDLSVYDIFGALSAGATLVLPDEARRRDPAHWAELIERHGVTLWNSVPALLEMLL 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2859 E-LRDF-ALPPGFKA-LCGGEALPENLATAL--LQKVTTLWNLYGPTETTIWSTLNGLTTPT------PYiGHPIANTQI 2927
Cdd:cd12114   233 DvLEAAqALLPSLRLvLLSGDWIPLDLPARLraLAPDARLISLGGATEASIWSIYHPIDEVPpdwrsiPY-GRPLANQRY 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2928 YILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPfsgaPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKV 3007
Cdd:cd12114   312 RVLDPRGRDCPDWVPGELWIGGRGVALGYLGDPELTAARFVTHP----DGERLYRTGDLGRYRPDGTLEFLGRRDGQVKV 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3008 RGFRIELGEIETRLARCHGVHDAVVIAReDSPGDKRLVAYLLAQPD-TVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPL 3086
Cdd:cd12114   388 RGYRIELGEIEAALQAHPGVARAVVVVL-GDPGGKRLAAFVVPDNDgTPIAPDALRAFLAQTLPAYMIPSRVIALEALPL 466

                         490
                  ....*....|.
gi 641744967 3087 TPNGKLDRKAL 3097
Cdd:cd12114   467 TANGKVDRAAL 477
AMP-binding pfam00501
AMP-binding enzyme;
1534-1932 8.64e-128

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 409.39  E-value: 8.64e-128
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1534 VEDQAARTPDTTAVL-FEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGY 1612
Cdd:pfam00501    1 LERQAARTPDKTALEvGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1613 PAERLAYMLDDASPVALLTQANQRA------------------LLTGDVPRILLDTADFSHLSEDNPHVPGLDAHHLAYV 1674
Cdd:pfam00501   81 PAEELAYILEDSGAKVLITDDALKLeellealgklevvklvlvLDRDPVLKEEPLPEEAKPADVPPPPPPPPDPDDLAYI 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1675 IYTSGSTGKPKGVMNSHRALCNRLVWMQNTY----RLTPDDRVLQKTPFSFDVSV-WEFFWPLLYGARLVMARPDGHKDA 1749
Cdd:pfam00501  161 IYTSGTTGKPKGVMLTHRNLVANVLSIKRVRprgfGLGPDDRVLSTLPLFHDFGLsLGLLGPLLAGATVVLPPGFPALDP 240

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1750 AYLAQLIERTGITTLHFVPSMLQQFVQ-WADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTF 1828
Cdd:pfam00501  241 AALLELIERYKVTVLYGVPTLLNMLLEaGAPKRALLSSLRLVLSGGAPLPPELARRFRELFGGALVNGYGLTETTGVVTT 320

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1829 WACQPDDHRSFVPIGRPIANTQLYILDTL-GQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDpfinqpgaRLYKT 1907
Cdd:pfam00501  321 PLPLDEDLRSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDED--------GWYRT 392

                          410       420
                   ....*....|....*....|....*
gi 641744967  1908 GDLARWLPDGSLEYLGRNDFQVKLR 1932
Cdd:pfam00501  393 GDLGRRDEDGYLEIVGRKKDQIKLG 417
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
 477-963 7.80e-125

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 401.69  E-value: 7.80e-125
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  477 QRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPA 556
Cdd:cd17653     1 DAFERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  557 YPAERLAYILDDAAPVALLTQSAqvaqlnstlptvlldtpaaaacPDTnpvvqglhaahLAYVIYTSGSTGRPKGVMVAH 636
Cdd:cd17653    81 LPSARIQAILRTSGATLLLTTDS----------------------PDD-----------LAYIIFTSGSTGIPKGVMVPH 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  637 RNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQLLS-GHTLVLvpdalrADAHQLWRYFARhAVDLFDCTPVQL 715
Cdd:cd17653   128 RGVLNYVSQPPARLDVGPGSRVAQVLSIAFDACIGEIFSTLCnGGTLVL------ADPSDPFAHVAR-TVDALMSTPSIL 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  716 QWLldaglgsDPAYQPA--QVLIGGEAISPavwsrlqSLSDT-----RFINVYGPTECTVDATACVVDRTQPLpTIGKPL 788
Cdd:cd17653   201 STL-------SPQDFPNlkTIFLGGEAVPP-------SLLDRwspgrRLYNAYGPTECTISSTMTELLPGQPV-TIGKPI 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  789 ANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsaDPAARIYKTGDLARWLPDGNIDYLGRND 868
Cdd:cd17653   266 PNSTCYILDADLQPVPEGVVGEICISGVQVARGYLGNPALTASKFVPDPF--WPGSRMYRTGDYGRWTEDGGLEFLGRED 343

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  869 FQIKVRGFRIEAGEIESRLLR-CPGVQDAVVIAREDspgdtRLVAYlcARPdAELHPAALRQQLAASLADYMIPSAFVTL 947
Cdd:cd17653   344 NQVKVRGFRINLEEIEEVVLQsQPEVTQAAAIVVNG-----RLVAF--VTP-ETVDVDGLRSELAKHLPSYAVPDRIIAL 415

                         490
                  ....*....|....*.
gi 641744967  948 DALPLTPNGKLDRKAL 963
Cdd:cd17653   416 DSFPLTANGKVDRKAL 431
Condensation pfam00668
Condensation domain; This domain is found in many multi-domain enzymes which synthesize ...
 1069-1512 9.36e-125

Condensation domain; This domain is found in many multi-domain enzymes which synthesize peptide antibiotics. This domain catalyzes a condensation reaction to form peptide bonds in non- ribosomal peptide biosynthesis. It is usually found to the carboxy side of a phosphopantetheine binding domain (pfam00550). It has been shown that mutations in the HHXXXDG motif abolish activity suggesting this is part of the active site.


Pssm-ID: 395541 [Multi-domain]  Cd Length: 454  Bit Score: 402.10  E-value: 9.36e-125
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1069 PLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFT-SIDGQPAQQIDpDTLGFSLS 1147
Cdd:pfam00668    6 PLSPAQKRMWFLEKLEPHSS-AYNMPAVLKLTGELDPERLEKALQELINRHDALRTVFIrQENGEPVQVIL-EERPFELE 83

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1148 SHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALE 1227
Cdd:pfam00668   84 IIDISDLSESEEEEAIEAFIQRDLQSPFDLEKGPLFRAGLFRIAENRHHLLLSMHHIIVDGVSLGILLRDLADLYQQLLK 163

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1228 GSEANLPPLPvQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHA 1307
Cdd:pfam00668  164 GEPLPLPPKT-PYKDYAEWLQQYLQSEDYQKDAAYWLEQLEGELPVLQLPKDYARPADRSFKGDRLSFTLDEDTEELLRK 242

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1308 LNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALD 1387
Cdd:pfam00668  243 LAKAHGTTLNDVLLAAYGLLLSRYTGQDDIVVGTPGSGRPSPDIERMVGMFVNTLPLRIDPKGGKTFSELIKRVQEDLLS 322

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1388 AYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQ-----ALTLPDLTLSAVERPQHSTHFDLSLSLIETENGLN 1462
Cdd:pfam00668  323 AEPHQGYPFGDLVNDLRLPRDLSRHPLFDPMFSFQNYLGQdsqeeEFQLSELDLSVSSVIEEEAKYDLSLTASERGGGLT 402

                          410       420       430       440       450
                   ....*....|....*....|....*....|....*....|....*....|
gi 641744967  1463 GGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPILPDSERRQ 1512
Cdd:pfam00668  403 IKIDYNTSLFDEETIERFAEHFKELLEQAIAHPSQPLSELDLLSDAEKQK 452
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
 2616-3097 1.65e-122

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 395.46  E-value: 1.65e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2616 VACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERL 2695
Cdd:cd05945     1 AAANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2696 RYMLDDAKPVALISqsahlgimngslpvillddgetrpfdnepdtpldarkqglTPRHLAYVIYTSGSTGKPKGVMVEHA 2775
Cdd:cd05945    81 REILDAAKPALLIA----------------------------------------DGDDNAYIIFTSGSTGRPKGVQISHD 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2776 NMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWR 2855
Cdd:cd05945   121 NLVSFTNWMLSDFPLGPGDVFLNQAPFSFDLSVMDLYPALASGATLVPVPRDATADPKQLFRFLAEHGITVWVSTPSFAA 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2856 MLVELRDFAlPPGFKAL-----CgGEALPENLATALLQKV--TTLWNLYGPTETTIWSTLNGLT-------TPTPyIGHP 2921
Cdd:cd05945   201 MCLLSPTFT-PESLPSLrhflfC-GEVLPHKTARALQQRFpdARIYNTYGPTEATVAVTYIEVTpevldgyDRLP-IGYA 277

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2922 IANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGApearmYKTGDLGRWLPDGTLEYLGRN 3001
Cdd:cd05945   278 KPGAKLVILDEDGRPVPPGEKGELVISGPSVSKGYLNNPEKTAAAFFPDEGQRA-----YRTGDLVRLEADGLLFYRGRL 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3002 DFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQP-DTVLEPADLRQRLSEGVAEYMIPSAFVT 3080
Cdd:cd05945   353 DFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPgAEAGLTKAIKAELAERLPPYMIPRRFVY 432

                         490
                  ....*....|....*..
gi 641744967 3081 LDAFPLTPNGKLDRKAL 3097
Cdd:cd05945   433 LDELPLNANGKIDRKAL 449
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
 483-963 2.99e-121

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 392.00  E-value: 2.99e-121
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:cd05945     1 AAANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLTQsaqvaqlnstlptvlldtpaaaacPDTNpvvqglhaahlAYVIYTSGSTGRPKGVMVAHRNVINL 642
Cdd:cd05945    81 REILDAAKPALLIAD------------------------GDDN-----------AYIIFTSGSTGRPKGVQISHDNLVSF 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  643 ATGLHTLLALDHPSRIALNASIVFDASVKNWI-QLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTP--VQLQwLL 719
Cdd:cd05945   126 TNWMLSDFPLGPGDVFLNQAPFSFDLSVMDLYpALASGATLVPVPRDATADPKQLFRFLAEHGITVWVSTPsfAAMC-LL 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  720 DAGLgsDPAYQPA--QVLIGGEAISPAVWSRLQS-LSDTRFINVYGPTECTVDATACVVDRTQ-----PLPtIGKPLANT 791
Cdd:cd05945   205 SPTF--TPESLPSlrHFLFCGEVLPHKTARALQQrFPDARIYNTYGPTEATVAVTYIEVTPEVldgydRLP-IGYAKPGA 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  792 RLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPfsadpAARIYKTGDLARWLPDGNIDYLGRNDFQI 871
Cdd:cd05945   282 KLVILDEDGRPVPPGEKGELVISGPSVSKGYLNNPEKTAAAFFPDE-----GQRAYRTGDLVRLEADGLLFYRGRLDFQV 356

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  872 KVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAE-LHPAALRQQLAASLADYMIPSAFVTLDAL 950
Cdd:cd05945   357 KLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPGAEaGLTKAIKAELAERLPPYMIPRRFVYLDEL 436

                         490
                  ....*....|...
gi 641744967  951 PLTPNGKLDRKAL 963
Cdd:cd05945   437 PLNANGKIDRKAL 449
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
 475-965 6.25e-121

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 391.10  E-value: 6.25e-121
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  475 IQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMD 554
Cdd:COG0318     1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  555 PAYPAERLAYILDDAAPVALLTqsaqvaqlnstlptvlldtpaaaacpdtnpvvqglhaahlAYVIYTSGSTGRPKGVMV 634
Cdd:COG0318    81 PRLTAEELAYILEDSGARALVT----------------------------------------ALILYTSGTTGRPKGVML 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  635 AHRNVINLATGLHTLLALDHPSRIALNASIVFDA--SVKNWIQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTP 712
Cdd:COG0318   121 THRNLLANAAAIAAALGLTPGDVVLVALPLFHVFglTVGLLAPLLAGATLVLLP---RFDPERVLELIERERVTVLFGVP 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  713 VQLQWLLDAGLGSDPAYQPAQVLI-GGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVD-RTQPLPTIGKPLAN 790
Cdd:COG0318   198 TMLARLLRHPEFARYDLSSLRLVVsGGAPLPPELLERFEERFGVRIVEGYGLTETSPVVTVNPEDpGERRPGSVGRPLPG 277

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  791 TRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFiPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQ 870
Cdd:COG0318   278 VEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWNDPEATAEAF-RDGW--------LRTGDLGRLDEDGYLYIVGRKKDM 348

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  871 IKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDAL 950
Cdd:COG0318   349 IISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAELDAEELRAFLRERLARYKVPRRVEFVDEL 428

                         490
                  ....*....|....*
gi 641744967  951 PLTPNGKLDRKALPA 965
Cdd:COG0318   429 PRTASGKIDRRALRE 443
AMP-binding pfam00501
AMP-binding enzyme;
479-874 7.11e-119

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 383.59  E-value: 7.11e-119
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   479 FEAQAEQTPEAIAVL-FEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAY 557
Cdd:pfam00501    1 LERQAARTPDKTALEvGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   558 PAERLAYILDDAAPVALLTQSAQVAQ--------LNSTLPTVLLDTPA----------AAACPDTNPVVQGLHAAHLAYV 619
Cdd:pfam00501   81 PAEELAYILEDSGAKVLITDDALKLEellealgkLEVVKLVLVLDRDPvlkeeplpeeAKPADVPPPPPPPPDPDDLAYI 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   620 IYTSGSTGRPKGVMVAHRNVINLATGL----HTLLALDHPSRIALNASIVFDASVKN--WIQLLSGHTLVLVPDALRADA 693
Cdd:pfam00501  161 IYTSGTTGKPKGVMLTHRNLVANVLSIkrvrPRGFGLGPDDRVLSTLPLFHDFGLSLglLGPLLAGATVVLPPGFPALDP 240

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   694 HQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQ-VLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATA 772
Cdd:pfam00501  241 AALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSSLRlVLSGGAPLPPELARRFRELFGGALVNGYGLTETTGVVTT 320

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   773 CV--VDRTQPLPTIGKPLANTRLYILDAQD-QPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKT 849
Cdd:pfam00501  321 PLplDEDLRSLGSVGRPLPGTEVKIVDDETgEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDEDGW--------YRT 392

                          410       420
                   ....*....|....*....|....*
gi 641744967   850 GDLARWLPDGNIDYLGRNDFQIKVR 874
Cdd:pfam00501  393 GDLGRRDEDGYLEIVGRKKDQIKLG 417
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
 2610-3097 6.60e-117

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 378.58  E-value: 6.60e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2610 ELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPT 2689
Cdd:cd17653     1 DAFERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2690 YPVERLRYMLDDAKPVALISQSAhlgimngslpvillddgetrpfdnepdtpldarkqgltPRHLAYVIYTSGSTGKPKG 2769
Cdd:cd17653    81 LPSARIQAILRTSGATLLLTTDS--------------------------------------PDDLAYIIFTSGSTGIPKG 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2770 VMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAmlierhaVSFMQA 2849
Cdd:cd17653   123 VMVPHRGVLNYVSQPPARLDVGPGSRVAQVLSIAFDACIGEIFSTLCNGGTLVLADPSDPFAHVART-------VDALMS 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2850 TPStwrMLVELRDFALPPGFKALCGGEALPENLATALLqKVTTLWNLYGPTETTIWSTLNGLTTPTP-YIGHPIANTQIY 2928
Cdd:cd17653   196 TPS---ILSTLSPQDFPNLKTIFLGGEAVPPSLLDRWS-PGRRLYNAYGPTECTISSTMTELLPGQPvTIGKPIPNSTCY 271

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2929 ILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSgaPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVR 3008
Cdd:cd17653   272 ILDADLQPVPEGVVGEICISGVQVARGYLGNPALTASKFVPDPFW--PGSRMYRTGDYGRWTEDGGLEFLGREDNQVKVR 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3009 GFRIELGEIE-TRLARCHGVHDAVVIAREDspgdkRLVAylLAQPDTVLEpADLRQRLSEGVAEYMIPSAFVTLDAFPLT 3087
Cdd:cd17653   350 GFRINLEEIEeVVLQSQPEVTQAAAIVVNG-----RLVA--FVTPETVDV-DGLRSELAKHLPSYAVPDRIIALDSFPLT 421

                         490
                  ....*....|
gi 641744967 3088 PNGKLDRKAL 3097
Cdd:cd17653   422 ANGKVDRKAL 431
AMP-binding pfam00501
AMP-binding enzyme;
2612-3008 2.46e-116

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 376.27  E-value: 2.46e-116
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2612 FEAQVACTPDAIAVVFGE-ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTY 2690
Cdd:pfam00501    1 LERQAARTPDKTALEVGEgRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2691 PVERLRYMLDDAKPVALISQSAHLGIMNGSL--------PVILLD-----DGETRPFDNEPDTPLDARKQGLTPRHLAYV 2757
Cdd:pfam00501   81 PAEELAYILEDSGAKVLITDDALKLEELLEAlgklevvkLVLVLDrdpvlKEEPLPEEAKPADVPPPPPPPPDPDDLAYI 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2758 IYTSGSTGKPKGVMVEHANMVNFLCSMRKEP----GIAQEDVLLGVTSLSFDIS-ILEIFLPLLNGARLILATQAQAADA 2832
Cdd:pfam00501  161 IYTSGTTGKPKGVMLTHRNLVANVLSIKRVRprgfGLGPDDRVLSTLPLFHDFGlSLGLLGPLLAGATVVLPPGFPALDP 240

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2833 QQLAMLIERHAVSFMQATPSTWRMLVELRDF--ALPPGFKA-LCGGEALPENLATALLQKV-TTLWNLYGPTETTIWST- 2907
Cdd:pfam00501  241 AALLELIERYKVTVLYGVPTLLNMLLEAGAPkrALLSSLRLvLSGGAPLPPELARRFRELFgGALVNGYGLTETTGVVTt 320

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2908 ---LNGLTTPTPYIGHPIANTQIYILDAQ-GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDpfsgapeaRMYKT 2983
Cdd:pfam00501  321 plpLDEDLRSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDED--------GWYRT 392

                          410       420
                   ....*....|....*....|....*
gi 641744967  2984 GDLGRWLPDGTLEYLGRNDFQVKVR 3008
Cdd:pfam00501  393 GDLGRRDEDGYLEIVGRKKDQIKLG 417
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
 2608-3099 2.13e-113

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 369.52  E-value: 2.13e-113
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLD 2687
Cdd:COG0318     1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2688 PTYPVERLRYMLDDAKPVALISqsahlgimngslpvillddgetrpfdnepdtpldarkqgltprhlAYVIYTSGSTGKP 2767
Cdd:COG0318    81 PRLTAEELAYILEDSGARALVT---------------------------------------------ALILYTSGTTGRP 115

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2768 KGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDIS-ILEIFLPLLNGARLILATQAQAADAQQlamLIERHAVSF 2846
Cdd:COG0318   116 KGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGlTVGLLAPLLAGATLVLLPRFDPERVLE---LIERERVTV 192

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2847 MQATPSTWRMLVELRDFA---LPPGFKALCGGEALPENLATALLQKV-TTLWNLYGPTETTIWSTLNGLTTPTPY---IG 2919
Cdd:COG0318   193 LFGVPTMLARLLRHPEFArydLSSLRLVVSGGAPLPPELLERFEERFgVRIVEGYGLTETSPVVTVNPEDPGERRpgsVG 272

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2920 HPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFitdpfsgapEARMYKTGDLGRWLPDGTLEYLG 2999
Cdd:COG0318   273 RPLPGVEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWNDPEATAEAF---------RDGWLRTGDLGRLDEDGYLYIVG 343

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3000 RNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFV 3079
Cdd:COG0318   344 RKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAELDAEELRAFLRERLARYKVPRRVE 423

                         490       500
                  ....*....|....*....|
gi 641744967 3080 TLDAFPLTPNGKLDRKALPA 3099
Cdd:COG0318   424 FVDELPRTASGKIDRRALRE 443
DCL_NRPS-like cd19536
DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal ...
 2161-2569 1.38e-109

DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal fungal CT domains and Dual Epimerization/Condensation (E/C) domains; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type [D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L))], which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380459 [Multi-domain]  Cd Length: 419  Bit Score: 357.14  E-value: 1.38e-109
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2161 IYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILPIN 2240
Cdd:cd19536     1 MYPLSSLQEGMLFHSLLNPGGSVYLHNYTYTVGRRLNLDLLLEALQVLIDRHDILRTSFIEDGLGQPVQVVHRQAQVPVT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2241 HFEPTSPEDVLAQLQAHTE-PRTRRIDLSQAPLFRADIAHDPLQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQAD- 2318
Cdd:cd19536    81 ELDLTPLEEQLDPLRAYKEeTKIRRFDLGRAPLVRAALVRKDERERFLLVISDHHSILDGWSLYLLVKEILAVYNQLLEy 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2319 ---ALPTPLPYRNFIAQTL-SVPNSAHEAYFRDKLADVDEPTAPFGLLNVQGsgGDIHEARLVLDATLASAIRQQARHLG 2394
Cdd:cd19536   161 kplSLPPAQPYRDFVAHERaSIQQAASERYWREYLAGATLATLPALSEAVGG--GPEQDSELLVSVPLPVRSRSLAKRSG 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2395 VSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADRIMGMFINTLPLRISLADRGAAEVVERTSHDLMTLLEHE 2474
Cdd:cd19536   239 IPLSTLLLAAWALVLSRHSGSDDVVFGTVVHGRSEETTGAERLLGLFLNTLPLRVTLSEETVEDLLKRAQEQELESLSHE 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2475 QAPLALAQRCSgvaPPMPLFSTLLNYRH-----TQASSTDNTLSDIRVLTSEERTNYPLTLAVDDR-GEGFSLVA---QT 2545
Cdd:cd19536   319 QVPLADIQRCS---EGEPLFDSIVNFRHfdldfGLPEWGSDEGMRRGLLFSEFKSNYDVNLSVLPKqDRLELKLAynsQV 395

                         410       420
                  ....*....|....*....|....
gi 641744967 2546 LEDIDPHRLLNYLMTAISSLVDAL 2569
Cdd:cd19536   396 LDEEQAQRLAAYYKSAIAELATAP 419
SgcC5_NRPS-like cd19539
SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- ...
 1069-1494 3.17e-107

SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- bond forming activity and similar C-domains of modular NRPSs; SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. This subfamily also includes similar C-domains of modular NRPSs such as Penicillium chrysogenum N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase PCBAB. Condensation (C) domains of NRPSs normally catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380462 [Multi-domain]  Cd Length: 427  Bit Score: 350.53  E-value: 3.17e-107
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1069 PLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSID-GQPAQQIDPDTlgfsls 1147
Cdd:cd19539     3 PLSFAQERLWFIDQGEDG-GPAYNIPGAWRLTGPLDVEALREALRDVVARHEALRTLLVRDDgGVPRQEILPPG------ 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1148 SHDLRKLDE----AARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQ 1223
Cdd:cd19539    76 PAPLEVRDLsdpdSDRERRLEELLRERESRGFDLDEEPPIRAVLGRFDPDDHVLVLVAHHTAFDAWSLDVFARDLAALYA 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1224 AALEGSEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGApALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLR 1303
Cdd:cd19539   156 ARRKGPAAPLPELRQQYKEYAAWQREALAAPRAAELLDFWRRRLRGA-EPTALPTDRPRPAGFPYPGADLRFELDAELVA 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1304 RLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRE 1383
Cdd:cd19539   235 ALRELAKRARSSLFMVLLAAYCVLLRRYTGQTDIVVGTPVAGRNHPRFESTVGFFVNLLPLRVDVSDCATFRDLIARVRK 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1384 RALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQALTLP-DLTLSAVERPQHSTHFDLSLSLIETENGLN 1462
Cdd:cd19539   315 ALVDAQRHQELPFQQLVAELPVDRDAGRHPLVQIVFQVTNAPAGELELAgGLSYTEGSDIPDGAKFDLNLTVTEEGTGLR 394

                         410       420       430
                  ....*....|....*....|....*....|..
gi 641744967 1463 GGLVYATDLFDRETILRVVGYVENILMAMADD 1494
Cdd:cd19539   395 GSLGYATSLFDEETIQGFLADYLQVLRQLLAN 426
DCL_NRPS-like cd19536
DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal ...
 28-436 2.27e-104

DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal fungal CT domains and Dual Epimerization/Condensation (E/C) domains; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type [D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L))], which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380459 [Multi-domain]  Cd Length: 419  Bit Score: 342.12  E-value: 2.27e-104
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   28 IYPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHQPVQVVWRQAPLTVN 107
Cdd:cd19536     1 MYPLSSLQEGMLFHSLLNPGGSVYLHNYTYTVGRRLNLDLLLEALQVLIDRHDILRTSFIEDGLGQPVQVVHRQAQVPVT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  108 TLTTTSSDTVPAQLRAATDPSNHR-LNLSNAPLLSATTAHDPVCGEWLLSLSIHHLISDHITQALIIDEIRL----LLED 182
Cdd:cd19536    81 ELDLTPLEEQLDPLRAYKEETKIRrFDLGRAPLVRAALVRKDERERFLLVISDHHSILDGWSLYLLVKEILAvynqLLEY 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  183 RPEALPKPLPYRNFIAQILSVPLS-EHEQYFRNRLADIDTPTAPFdLVDVQGnGEDITEARLSLDSSLADALRRQARHLG 261
Cdd:cd19536   161 KPLSLPPAQPYRDFVAHERASIQQaASERYWREYLAGATLATLPA-LSEAVG-GGPEQDSELLVSVPLPVRSRSLAKRSG 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  262 ISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGADRVMGMFINTLPLRVSLRERSVHDVVQATSHELMMLLAHE 341
Cdd:cd19536   239 IPLSTLLLAAWALVLSRHSGSDDVVFGTVVHGRSEETTGAERLLGLFLNTLPLRVTLSEETVEDLLKRAQEQELESLSHE 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  342 QAPLALAQQCSQVPpplPLFSTLFNYRHSQKDASSQFW-----EGMRQLSGRERTNYPITLSVDDLGD----GFNLTAKT 412
Cdd:cd19536   319 QVPLADIQRCSEGE---PLFDSIVNFRHFDLDFGLPEWgsdegMRRGLLFSEFKSNYDVNLSVLPKQDrlelKLAYNSQV 395

                         410       420
                  ....*....|....*....|....
gi 641744967  413 VMGVDPERIVHYMLTAIENLVTSL 436
Cdd:cd19536   396 LDEEQAQRLAAYYKSAIAELATAP 419
alpha_am_amid TIGR03443
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ...
 1263-2106 3.58e-97

L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.


Pssm-ID: 274582 [Multi-domain]  Cd Length: 1389  Bit Score: 347.44  E-value: 3.58e-97
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1263 WRDQLQgAPALLEIPTDRPRPSVQRYAgdQVPFHLDAGQLRRLHAlnrqQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTP 1342
Cdd:TIGR03443    2 WSERLD-NPTLSVLPHDYLRPANNRLV--EATYSLQLPSAEVTAG----GGSTPFIILLAAFAALVYRLTGDEDIVLGTS 74

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1343 VANRPRQelegmvgffvntLALRTEPGRCHAVADLLDQVRERALDAYAHQALPFEQVVEILQPARSL-SYSPIFQVmlsl 1421
Cdd:TIGR03443   75 SNKSGRP------------FVLRLNITPELSFLQLYAKVSEEEKEGASDIGVPFDELSEHIQAAKKLeRTPPLFRL---- 138

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1422 nntpaQALTLPDLTLSAVErpQHSThFDLSLSLIETENGLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVAT 1501
Cdd:TIGR03443  139 -----AFQDAPDNQQTTYS--TGST-TDLTVFLTPSSPELELSIYYNSLLFSSDRITIVADQLAQLLSAASSNPDEPIGK 210

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1502 LPILPDSERRqvMLDFNATEADFP-HDALIHQLVEDQAARTPDTTAVLFEDQHL---------TYDALNRRANQLAHHLI 1571
Cdd:TIGR03443  211 VSLITPSQKS--LLPDPTKDLDWSgFRGAIHDIFADNAEKHPDRTCVVETPSFLdpssktrsfTYKQINEASNILAHYLL 288

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1572 DLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVAL--LTQANQRALLTGD------- 1642
Cdd:TIGR03443  289 KTGIKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQTIYLSVAKPRALivIEKAGTLDQLVRDyidkele 368

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1643 ----VPRI-LLDTADFSHLSEDNPHVPGLdAHHLAY------VI----------YTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:TIGR03443  369 lrteIPALaLQDDGSLVGGSLEGGETDVL-APYQALkdtptgVVvgpdsnptlsFTSGSEGIPKGVLGRHFSLAYYFPWM 447

                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1702 QNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVqwADAD 1781
Cdd:TIGR03443  448 AKRFGLSENDKFTMLSGIAHDPIQRDMFTPLFLGAQLLVPTADDIGTPGRLAEWMAKYGATVTHLTPAMGQLLS--AQAT 525

                          570       580       590       600       610       620       630       640
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1782 CACDSLRRVICSGEALPAE----LQQrfFARfNAQLHNLYGPTEA------------AIDVTFWACQPDdhrsFVPIGRP 1845
Cdd:TIGR03443  526 TPIPSLHHAFFVGDILTKRdclrLQT--LAE-NVCIVNMYGTTETqravsyfeipsrSSDSTFLKNLKD----VMPAGKG 598

                          650       660       670       680       690       700       710       720
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1846 IANTQLYIL---DTlGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFI-----------NQPGA--------- 1902
Cdd:TIGR03443  599 MKNVQLLVVnrnDR-TQTCGVGEVGEIYVRAGGLAEGYLGLPELNAEKFVNNWFVdpshwidldkeNNKPErefwlgprd 677

                          730       740       750       760       770       780       790       800
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1903 RLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPG----- 1977
Cdd:TIGR03443  678 RLYRTGDLGRYLPDGNVECCGRADDQVKIRGFRIELGEIDTHLSQHPLVRENVTLVRRDKDEEPTLVSYIVPQDKsdele 757

                          810       820       830       840       850       860       870       880
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1978 ---------VTPDPA------------DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAPDQ---SAVATRD 2033
Cdd:TIGR03443  758 efksevddeESSDPVvkglikyrklikDIREYLKKKLPSYAIPTVIVPLKKLPLNPNGKVDKPALPFPDTaqlAAVAKNR 837

                          890       900       910       920       930       940       950       960
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2034 Y----EAPQGEVETALAAVWQDLL--GLTRVGRHDHFFALGGHSLLIVSLIERLRRAgLALDVR--GVFSTPVLSDMAQA 2105
Cdd:TIGR03443  838 SasaaDEEFTETEREIRDLWLELLpnRPATISPDDSFFDLGGHSILATRMIFELRKK-LNVELPlgLIFKSPTIKGFAKE 916


                   .
gi 641744967  2106 I 2106
Cdd:TIGR03443  917 V 917
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
1529-2021 6.17e-93

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 312.21  E-value: 6.17e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1529 LIHQLvEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLgvKPDDRIAICV--ERSLDMVIGLLAILKAGAAYV 1606
Cdd:PRK04813    4 IIETI-EEFAQTQPDFPAYDYLGEKLTYGQLKEDSDALAAFIDSL--KLPDKSPIIVfgHMSPEMLATFLGAVKAGHAYI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1607 PLDPGYPAERLAYMLDDASPVALLTQANQRALLTgDVPRILLD----------TADFSHlsednpHVPGLDAhhlAYVIY 1676
Cdd:PRK04813   81 PVDVSSPAERIEMIIEVAKPSLIIATEELPLEIL-GIPVITLDelkdifatgnPYDFDH------AVKGDDN---YYIIF 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1677 TSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLI 1756
Cdd:PRK04813  151 TSGTTGKPKGVQISHDNLVSFTNWMLEDFALPEGPQFLNQAPYSFDLSVMDLYPTLASGGTLVALPKDMTANFKQLFETL 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1757 ERTGITTLHFVPSmlqqFVQWADADCACD-----SLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWA 1830
Cdd:PRK04813  231 PQLPINVWVSTPS----FADMCLLDPSFNeehlpNLTHFLFCGEELPHKTAKKLLERFpSATIYNTYGPTEATVAVTSIE 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1831 CQPD--DHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPfinqpGARLYKTG 1908
Cdd:PRK04813  307 ITDEmlDQYKRLPIGYAKPDSPLLIIDEEGTKLPDGEQGEIVISGPSVSKGYLNNPEKTAEAFFTFD-----GQPAYHTG 381

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1909 DLARwLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADL--- 1985
Cdd:PRK04813  382 DAGY-LEDGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVESAVVVPYNKDHKVQYLIAYVVPKEEDFEREFELtka 460

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 641744967 1986 -RQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK04813  461 iKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKAL 497
C_PKS-NRPS cd20483
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 1067-1479 2.92e-89

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHXXXD motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380471 [Multi-domain]  Cd Length: 430  Bit Score: 298.79  E-value: 2.92e-89
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1067 PLPLSFSQQRLWFLAQL--DPAAsqaYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIdPDTLGF 1144
Cdd:cd20483     1 PRPMSTFQRRLWFLHNFleDKTF---LNLLLVCHIKGKPDVNLLQKALSELVRRHEVLRTAYFEGDDFGEQQV-LDDPSF 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1145 SLSSHDLRklDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQA 1224
Cdd:cd20483    77 HLIVIDLS--EAADPEAALDQLVRNLRRQELDIEEGEVIRGWLVKLPDEEFALVLASHHIAWDRGSSKSIFEQFTALYDA 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1225 ALEG-SEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPA---LLEIPTdRPRPSVQRYAGDQVPFHLDAG 1300
Cdd:cd20483   155 LRAGrDLATVPPPPVQYIDFTLWHNALLQSPLVQPLLDFWKEKLEGIPDaskLLPFAK-AERPPVKDYERSTVEATLDKE 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1301 QLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQ 1380
Cdd:cd20483   234 LLARMKRICAQHAVTPFMFLLAAFRAFLYRYTEDEDLTIGMVDGDRPHPDFDDLVGFFVNMLPIRCRMDCDMSFDDLLES 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1381 VRERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLS--LNNTPAQALTlPDLTLSAVERPQHSTHFDLSLSLIET- 1457
Cdd:cd20483   314 TKTTCLEAYEHSAVPFDYIVDALDVPRSTSHFPIGQIAVNyqVHGKFPEYDT-GDFKFTDYDHYDIPTACDIALEAEEDp 392

                         410       420
                  ....*....|....*....|..
gi 641744967 1458 ENGLNGGLVYATDLFDRETILR 1479
Cdd:cd20483   393 DGGLDLRLEFSTTLYDSADMER 414
C_NRPS-like cd19066
Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of ...
 1067-1492 5.19e-88

Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long, with various activities such as antibiotic, antifungal, antitumor and immunosuppression. There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380453 [Multi-domain]  Cd Length: 427  Bit Score: 295.09  E-value: 5.19e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1067 PLPLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLGFSL 1146
Cdd:cd19066     1 KIPLSPMQRGMWFLKKLATDPS-AFNVAIEMFLTGSLDLARLKQALDAVMERHDVLRTRFCEEAGRYEQVVLDKTVRFRI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1147 SSHDLRKLDEaaRTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAAL 1226
Cdd:cd19066    80 EIIDLRNLAD--PEARLLELIDQIQQTIYDLERGPLVRVALFRLADERDVLVVAIHHIIVDGGSFQILFEDISSVYDAAE 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1227 EGSEAnLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLH 1306
Cdd:cd19066   158 RQKPT-LPPPVGSYADYAAWLEKQLESEAAQADLAYWTSYLHGLPPPLPLPKAKRPSQVASYEVLTLEFFLRSEETKRLR 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1307 ALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERAL 1386
Cdd:cd19066   237 EVARESGTTPTQLLLAAFALALKRLTASIDVVIGLTFLNRPDEAVEDTIGLFLNLLPLRIDTSPDATFPELLKRTKEQSR 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1387 DAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTPAQALTLPDLTLSAVE-RPQHSTHFDLSLSLIETENG-LNGG 1464
Cdd:cd19066   317 EAIEHQRVPFIELVRHLGVVPEAPKHPLFEPVFTFKNNQQQLGKTGGFIFTTPVyTSSEGTVFDLDLEASEDPDGdLLLR 396

                         410       420
                  ....*....|....*....|....*...
gi 641744967 1465 LVYATDLFDRETILRvvgYVENILMAMA 1492
Cdd:cd19066   397 LEYSRGVYDERTIDR---FAERYMTALR 421
COG4908 COG4908
Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General ...
 1070-1317 5.90e-88

Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General function prediction only];


Pssm-ID: 443936 [Multi-domain]  Cd Length: 243  Bit Score: 287.71  E-value: 5.90e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1070 LSFSQQRLWFLAQldpaASQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDtLGFSLSSH 1149
Cdd:COG4908     1 LSPAQKRFLFLEP----GSNAYNIPAVLRLEGPLDVEALERALRELVRRHPALRTRFVEEDGEPVQRIDPD-ADLPLEVV 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1150 DLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEGS 1229
Cdd:COG4908    76 DLSALPEPEREAELEELVAEEASRPFDLARGPLLRAALIRLGEDEHVLLLTIHHIISDGWSLGILLRELAALYAALLEGE 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1230 EANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHALN 1309
Cdd:COG4908   156 PPPLPELPIQYADYAAWQRAWLQSEALEKQLEYWRQQLAGAPPVLELPTDRPRPAVQTFRGATLSFTLPAELTEALKALA 235


                  ....*...
gi 641744967 1310 RQQGTTLF 1317
Cdd:COG4908   236 KAHGATVN 243
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
473-963 2.21e-87

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 296.42  E-value: 2.21e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  473 MLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALgvQPDDRVALCV--ERSLEMMVGLLGILKAGAAY 550
Cdd:PRK04813    2 MDIIETIEEFAQTQPDFPAYDYLGEKLTYGQLKEDSDALAAFIDSL--KLPDKSPIIVfgHMSPEMLATFLGAVKAGHAY 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  551 VPMDPAYPAERLAYILDDAAPVALLTqsaqVAQLNSTL-------PTVLLDTPAAAACPDTNPVVQGlhaAHLAYVIYTS 623
Cdd:PRK04813   80 IPVDVSSPAERIEMIIEVAKPSLIIA----TEELPLEIlgipvitLDELKDIFATGNPYDFDHAVKG---DDNYYIIFTS 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  624 GSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWI-QLLSGHTLVLVPDALRADAHQLWRYFAR 702
Cdd:PRK04813  153 GTTGKPKGVQISHDNLVSFTNWMLEDFALPEGPQFLNQAPYSFDLSVMDLYpTLASGGTLVALPKDMTANFKQLFETLPQ 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  703 HAVDLFDCTP--VQLQwLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRL-QSLSDTRFINVYGPTECTVDATAC-----V 774
Cdd:PRK04813  233 LPINVWVSTPsfADMC-LLDPSFNEEHLPNLTHFLFCGEELPHKTAKKLlERFPSATIYNTYGPTEATVAVTSIeitdeM 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  775 VDRTQPLPtIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFipdpFSADpAARIYKTGDLAR 854
Cdd:PRK04813  312 LDQYKRLP-IGYAKPDSPLLIIDEEGTKLPDGEQGEIVISGPSVSKGYLNNPEKTAEAF----FTFD-GQPAYHTGDAGY 385

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  855 wLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARP-----DAELhPAALRQ 929
Cdd:PRK04813  386 -LEDGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVESAVVVPYNKDHKVQYLIAYVVPKEedferEFEL-TKAIKK 463

                         490       500       510
                  ....*....|....*....|....*....|....
gi 641744967  930 QLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK04813  464 ELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKAL 497
D-ala-DACP-lig TIGR01734
D-alanine--poly(phosphoribitol) ligase, subunit 1; This model represents the enzyme (also ...
 1529-2021 9.74e-86

D-alanine--poly(phosphoribitol) ligase, subunit 1; This model represents the enzyme (also called D-alanine-D-alanyl carrier protein ligase) which activates D-alanine as an adenylate via the reaction D-ala + ATP -> D-ala-AMP + PPi, and further catalyzes the condensation of the amino acid adenylate with the D-alanyl carrier protein (D-ala-ACP). The D-alanine is then further transferred to teichoic acid in the biosynthesis of lipoteichoic acid (LTA) and wall teichoic acid (WTA) in gram positive bacteria, both polysacchatides. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273780 [Multi-domain]  Cd Length: 502  Bit Score: 291.28  E-value: 9.74e-86
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1529 LIHQLvEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPL 1608
Cdd:TIGR01734    2 LIEAI-QAFAETYPQTIAYRYQGQELTYQQLKEQSDRLAAFIQKRILPKKSPIIVYGHMEPHMLVAFLGSIKSGHAYIPV 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1609 DPGYPAERLAYMLDDASPVALLTQANQRALLTgDVPRI---LLDTADFSHLSEDNPH-VPGLDAHhlaYVIYTSGSTGKP 1684
Cdd:TIGR01734   81 DTSIPSERIEMIIEAAGPELVIHTAELSIDAV-GTQIItlsALEQAETSGGPVSFDHaVKGDDNY---YIIYTSGSTGNP 156

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1685 KGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTL 1764
Cdd:TIGR01734  157 KGVQISHDNLVSFTNWMLADFPLSEGKQFLNQAPFSFDLSVMDLYPCLASGGTLHCLDKDITNNFKLLFEELPKTGLNVW 236

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1765 HFVPSmlqqFVQWADADCACD-----SLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWACQPD--DH 1836
Cdd:TIGR01734  237 VSTPS----FVDMCLLDPNFNqenypHLTHFLFCGEELPVKTAKALLERFpKATIYNTYGPTEATVAVTSVKITQEilDQ 312

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1837 RSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDpfinqPGARLYKTGDLARwLPD 1916
Cdd:TIGR01734  313 YPRLPIGFAKPDMNLFIMDEEGEPLPEGEKGEIVIVGPSVSKGYLNNPEKTAEAFFSH-----EGQPAYRTGDAGT-ITD 386

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1917 GSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGD-TRLVAYLCPQPGVTPDPADL----RQQLGQ 1991
Cdd:TIGR01734  387 GQLFYQGRLDFQIKLHGYRIELEDIEFNLRQSSYIESAVVVPKYNKDHKvEYLIAAIVPETEDFEKEFQLtkaiKKELKK 466

                          490       500       510
                   ....*....|....*....|....*....|
gi 641744967  1992 HLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:TIGR01734  467 SLPAYMIPRKFIYRDQLPLTANGKIDRKAL 496
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
2607-3097 2.32e-85

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 290.26  E-value: 2.32e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2607 LIHELFE-AQVacTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGvrPDERVAICVERGL--DMVVGLLGILKAGGAY 2683
Cdd:PRK04813    4 IIETIEEfAQT--QPDFPAYDYLGEKLTYGQLKEDSDALAAFIDSLK--LPDKSPIIVFGHMspEMLATFLGAVKAGHAY 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2684 VPLDPTYPVERLRYMLDDAKPVALISQSAhLGIMNGSLPVILLDDGETRPFDNEPDTPLDARKQGLTprhlAYVIYTSGS 2763
Cdd:PRK04813   80 IPVDVSSPAERIEMIIEVAKPSLIIATEE-LPLEILGIPVITLDELKDIFATGNPYDFDHAVKGDDN----YYIIFTSGT 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2764 TGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHA 2843
Cdd:PRK04813  155 TGKPKGVQISHDNLVSFTNWMLEDFALPEGPQFLNQAPYSFDLSVMDLYPTLASGGTLVALPKDMTANFKQLFETLPQLP 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2844 VSFMQATPSTWRMLVELRDF--ALPPGFKA--LCGgEALPENLATALLQKV--TTLWNLYGPTETT-----IWSTLNGLT 2912
Cdd:PRK04813  235 INVWVSTPSFADMCLLDPSFneEHLPNLTHflFCG-EELPHKTAKKLLERFpsATIYNTYGPTEATvavtsIEITDEMLD 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2913 T--PTPyIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITdpFSGAPEarmYKTGDLGRwL 2990
Cdd:PRK04813  314 QykRLP-IGYAKPDSPLLIIDEEGTKLPDGEQGEIVISGPSVSKGYLNNPEKTAEAFFT--FDGQPA---YHTGDAGY-L 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2991 PDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIaredsPGDK-----RLVAYLLAQPDTVLEPADL---- 3061
Cdd:PRK04813  387 EDGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVESAVVV-----PYNKdhkvqYLIAYVVPKEEDFEREFELtkai 461

                         490       500       510
                  ....*....|....*....|....*....|....*.
gi 641744967 3062 RQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK04813  462 KKELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKAL 497
DCL_NRPS cd19543
DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the ...
 1068-1494 4.00e-84

DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor; The DCL-type Condensation (C) domain catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. This domain is D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains in addition to the LCL- and DCL-types such as starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380465 [Multi-domain]  Cd Length: 423  Bit Score: 283.71  E-value: 4.00e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1068 LPLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTS-IDGQPAQQIDPDtLGFSL 1146
Cdd:cd19543     2 YPLSPMQEGMLFHSLLDPG-SGAYVEQMVITLEGPLDPDRFRAAWQAVVDRHPILRTSFVWeGLGEPLQVVLKD-RKLPW 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1147 SSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAAL 1226
Cdd:cd19543    80 RELDLSHLSEAEQEAELEALAEEDRERGFDLARAPLMRLTLIRLGDDRYRLVWSFHHILLDGWSLPILLKELFAIYAALG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1227 EGSEANLPPlPVQYADYAvwqrQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLH 1306
Cdd:cd19543   160 EGQPPSLPP-VRPYRDYI----AWLQRQDKEAAEAYWREYLAGFEEPTPLPKELPADADGSYEPGEVSFELSAELTARLQ 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1307 ALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPrQELEG---MVGFFVNTLALRTEPGRCHAVADLLDQVRE 1383
Cdd:cd19543   235 ELARQHGVTLNTVVQGAWALLLSRYSGRDDVVFGTTVSGRP-AELPGietMVGLFINTLPVRVRLDPDQTVLELLKDLQA 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1384 RALDAYAHQALPFeqvVEIlQpARSLSYSPIFQVMLSLNNTP--AQALTLPD---LTLSAVERPQHsTHFDLSLSLIETE 1458
Cdd:cd19543   314 QQLELREHEYVPL---YEI-Q-AWSEGKQALFDHLLVFENYPvdESLEEEQDedgLRITDVSAEEQ-TNYPLTVVAIPGE 387

                         410       420       430
                  ....*....|....*....|....*....|....*.
gi 641744967 1459 nGLNGGLVYATDLFDRETILRVVGYVENILMAMADD 1494
Cdd:cd19543   388 -ELTIKLSYDAEVFDEATIERLLGHLRRVLEQVAAN 422
alpha_am_amid TIGR03443
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ...
 2372-3180 1.13e-83

L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.


Pssm-ID: 274582 [Multi-domain]  Cd Length: 1389  Bit Score: 305.07  E-value: 1.13e-83
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2372 HEARLVlDATLASAIRQQARHL--GVSPGVLFHVAWAQVLAQTSGRDDVVFGSvllgrlAGAEGADRimgmFINTLPLRI 2449
Cdd:TIGR03443   22 ANNRLV-EATYSLQLPSAEVTAggGSTPFIILLAAFAALVYRLTGDEDIVLGT------SSNKSGRP----FVLRLNITP 90

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2450 SLAdrgAAEVVERTSHDLMTLLEHEQAPLA-LA---QRCSGVAPPMPLFStlLNYRHTQASSTDN----TLSDIRVLTSE 2521
Cdd:TIGR03443   91 ELS---FLQLYAKVSEEEKEGASDIGVPFDeLSehiQAAKKLERTPPLFR--LAFQDAPDNQQTTystgSTTDLTVFLTP 165

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2522 ERTNYPLTLAVDDRGegFSlvaqtledidpHRLLNYLMTAISSLVDALETEPQRSILNLPVLPDSERQqMLVDfNATDAD 2601
Cdd:TIGR03443  166 SSPELELSIYYNSLL--FS-----------SDRITIVADQLAQLLSAASSNPDEPIGKVSLITPSQKS-LLPD-PTKDLD 230

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2602 IP--RHAlIHELFEAQVACTPDAIAVVFGEASL---------SYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMV 2670
Cdd:TIGR03443  231 WSgfRGA-IHDIFADNAEKHPDRTCVVETPSFLdpssktrsfTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLV 309

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2671 VGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALI--------SQSAHLGImNGSLPVI-------LLDDGET---R 2732
Cdd:TIGR03443  310 VAVMGVLKAGATFSVIDPAYPPARQTIYLSVAKPRALIviekagtlDQLVRDYI-DKELELRteipalaLQDDGSLvggS 388

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2733 PFDNEPDT--PLDARKQGLT-----PRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFD 2805
Cdd:TIGR03443  389 LEGGETDVlaPYQALKDTPTgvvvgPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFPWMAKRFGLSENDKFTMLSGIAHD 468

                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2806 ISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELRDFALPPGFKALCGGEALPENLATA 2885
Cdd:TIGR03443  469 PIQRDMFTPLFLGAQLLVPTADDIGTPGRLAEWMAKYGATVTHLTPAMGQLLSAQATTPIPSLHHAFFVGDILTKRDCLR 548

                          570       580       590       600       610       620       630       640
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2886 L--LQKVTTLWNLYGPTET-------TIWST------LNGLTTPTPyIGHPIANTQIYILDAQGRVVPLGVA--GEIHIA 2948
Cdd:TIGR03443  549 LqtLAENVCIVNMYGTTETqravsyfEIPSRssdstfLKNLKDVMP-AGKGMKNVQLLVVNRNDRTQTCGVGevGEIYVR 627

                          650       660       670       680       690       700       710       720
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2949 GAGVVRGYLGRPDLTAERFIT------------DPFSGAPEA--------RMYKTGDLGRWLPDGTLEYLGRNDFQVKVR 3008
Cdd:TIGR03443  628 AGGLAEGYLGLPELNAEKFVNnwfvdpshwidlDKENNKPERefwlgprdRLYRTGDLGRYLPDGNVECCGRADDQVKIR 707

                          730       740       750       760       770       780       790       800
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  3009 GFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTV-LEPA-------------------------DLR 3062
Cdd:TIGR03443  708 GFRIELGEIDTHLSQHPLVRENVTLVRRDKDEEPTLVSYIVPQDKSDeLEEFksevddeessdpvvkglikyrklikDIR 787

                          810       820       830       840       850       860       870       880
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  3063 QRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPD--QSAMATRGYEAPQGD-----LEHALAQIWQTLL--GVER 3133
Cdd:TIGR03443  788 EYLKKKLPSYAIPTVIVPLKKLPLNPNGKVDKPALPFPDtaQLAAVAKNRSASAADeefteTEREIRDLWLELLpnRPAT 867

                          890       900       910       920
                   ....*....|....*....|....*....|....*....|....*..
gi 641744967  3134 VGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALA 3180
Cdd:TIGR03443  868 ISPDDSFFDLGGHSILATRMIFELRKKLNVELPLGLIFKSPTIKGFA 914
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
 1670-2017 1.15e-83

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 279.17  E-value: 1.15e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1670 HLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDghkDA 1749
Cdd:cd04433     1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPKF---DP 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1750 AYLAQLIERTGITTLHFVPSMLQQFV-QWADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTF 1828
Cdd:cd04433    78 EAALELIEREKVTILLGVPTLLARLLkAPESAGYDLSSLRALVSGGAPLPPELLERFEEAPGIKLVNGYGLTETGGTVAT 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1829 WaCQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFipdpfinqpGARLYKTG 1908
Cdd:cd04433   158 G-PPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAAVD---------EDGWYRTG 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1909 DLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQ 1988
Cdd:cd04433   228 DLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAEELRAH 307

                         330       340
                  ....*....|....*....|....*....
gi 641744967 1989 LGQHLAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:cd04433   308 VRERLAPYKVPRRVVFVDALPRTASGKID 336
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
1531-2021 2.77e-82

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 283.54  E-value: 2.77e-82
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQAARTPDTTAVLFED-----QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAY 1605
Cdd:COG0365    12 YNCLDRHAEGRGDKVALIWEGedgeeRTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVH 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1606 VPLDPGYPAERLAYMLDDASPVALLTQAnqrALLTGDVPRILLDTAD--------------FSHLSEDnPHVPG------ 1665
Cdd:COG0365    92 SPVFPGFGAEALADRIEDAEAKVLITAD---GGLRGGKVIDLKEKVDealeelpslehvivVGRTGAD-VPMEGdldwde 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1666 -------------LDAHHLAYVIYTSGSTGKPKGVMNSHRALcnrLVW----MQNTYRLTPDDRVLQKTPFSFDVSVW-E 1727
Cdd:COG0365   168 llaaasaefepepTDADDPLFILYTSGTTGKPKGVVHTHGGY---LVHaattAKYVLDLKPGDVFWCTADIGWATGHSyI 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1728 FFWPLLYGARLVM--ARPDgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWAD---ADCACDSLRRVICSGEALPAELQ 1802
Cdd:COG0365   245 VYGPLLNGATVVLyeGRPD-FPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDeplKKYDLSSLRLLGSAGEPLNPEVW 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1803 QRFFARFNAQLHNLYGPTEA-AIDVTFWACQPddhrsFVP--IGRPIANTQLYILDTLGQPVPLGVAGELHIGG--VGVA 1877
Cdd:COG0365   324 EWWYEAVGVPIVDGWGQTETgGIFISNLPGLP-----VKPgsMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGpwPGMF 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1878 RGYLNRPDLTAERFipdpFINQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVV 1957
Cdd:COG0365   399 RGYWNDPERYRETY----FGRFPG--WYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVV 472

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 1958 AREDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:COG0365   473 GVPDEIRGQVVKAFVVLKPGVEPSDElakELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLL 539
X-Domain_NRPS cd19546
X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to ...
 1064-1494 3.17e-82

X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to the non-ribosomal peptide synthetase (NRPS); The X-domain is a catalytically inactive member of the Condensation (C) domain family of non-ribosomal peptide synthetase (NRPS). It has been shown to recruit oxygenases to the NRPS to perform side-chain crosslinking in the production of glycopeptide antibiotics. C-domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as this X-domain, the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, and dual E/C (epimerization and condensation) domains. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity; members of this X-domain subfamily lack the second H of this motif.


Pssm-ID: 380468 [Multi-domain]  Cd Length: 440  Bit Score: 278.98  E-value: 3.17e-82
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1064 RTQPLPLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQI-DPDTL 1142
Cdd:cd19546     1 RPDEVPATAGQLRTWLLARLDEE-TRGRHLSVALRLRGRLDRDALEAALGDVAARHEILRTTFPGDGGDVHQRIlDADAA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1143 GFSLsshdlrKLDEAARTTRVAELAEQEARaRFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALY 1222
Cdd:cd19546    80 RPEL------PVVPATEEELPALLADRAAH-LFDLTRETPWRCTLFALSDTEHVLLLVVHRIAADDESLDVLVRDLAAAY 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1223 QAALEGSEANLPPLPVQYADYAVWQRQWLQGET-----LNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHL 1297
Cdd:cd19546   153 GARREGRAPERAPLPLQFADYALWERELLAGEDdrdslIGDQIAYWRDALAGAPDELELPTDRPRPVLPSRRAGAVPLRL 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1298 DAGQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQ-ELEGMVGFFVNTLALRTEPGRCHAVAD 1376
Cdd:cd19546   233 DAEVHARLMEAAESAGATMFTVVQAALAMLLTRLGAGTDVTVGTVLPRDDEEgDLEGMVGPFARPLALRTDLSGDPTFRE 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1377 LLDQVRERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSL---NNTPAQALTLPDLTLSAVERPQHSTHFDLSLS 1453
Cdd:cd19546   313 LLGRVREAVREARRHQDVPFERLAELLALPPSADRHPVFQVALDVrddDNDPWDAPELPGLRTSPVPLGTEAMELDLSLA 392

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*..
gi 641744967 1454 LIETEN------GLNGGLVYATDLFDRETILRVVGYVENILMAMADD 1494
Cdd:cd19546   393 LTERRNddgdpdGLDGSLRYAADLFDRATAAALARRLVRVLEQVAAD 439
ligase_PEP_1 TIGR03098
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ...
 1529-2023 1.62e-81

acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.


Pssm-ID: 211788 [Multi-domain]  Cd Length: 517  Bit Score: 279.74  E-value: 1.62e-81
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1529 LIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPL 1608
Cdd:TIGR03098    1 LLHHLLEDAAARLPDATALVHHDRTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPI 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1609 DPGYPAERLAYMLDDASPVALLTQANQRALLTGDVP-----RILLDTADFSHLSEDNPH------------------VPG 1665
Cdd:TIGR03098   81 NPLLKAEQVAHILADCNVRLLVTSSERLDLLHPALPgchdlRTLIIVGDPAHASEGHPGeepaswpkllalgdadppHPV 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1666 LDAHhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDG 1745
Cdd:TIGR03098  161 IDSD-MAAILYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGATVVLHDYLL 239

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1746 HKDaayLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEaAI 1824
Cdd:TIGR03098  240 PRD---VLKALEKHGITGLAAVPPLWAQLAQLDWPESAAPSLRYLTNSGGAMPRATLSRLRSFLpNARLFLMYGLTE-AF 315

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1825 DVTFWACQPDDHRSfVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPfINQPGARL 1904
Cdd:TIGR03098  316 RSTYLPPEEVDRRP-DSIGKAIPNAEVLVLREDGSECAPGEEGELVHRGALVAMGYWNDPEKTAERFRPLP-PFPGELHL 393

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1905 YKT----GDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTP 1980
Cdd:TIGR03098  394 PELavwsGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTLGQAIVLVVTPPGGEEL 473

                          490       500       510       520
                   ....*....|....*....|....*....|....*....|...
gi 641744967  1981 DPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:TIGR03098  474 DRAALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
 1532-2021 2.54e-81

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 277.52  E-value: 2.54e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1532 QLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:cd05936     3 DLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 YPAERLAYMLDDASPVALLTqanqralltgdvprilldTADFSHL---SEDNPHVPGLDAHHLAYVIYTSGSTGKPKGVM 1688
Cdd:cd05936    83 YTPRELEHILNDSGAKALIV------------------AVSFTDLlaaGAPLGERVALTPEDVAVLQYTSGTTGVPKGAM 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1689 NSHRAL-CNRLV---WMQNtyRLTPDDRVLQKTP----FSFDVSVwefFWPLLYGARLVM---ARPDGhkdaayLAQLIE 1757
Cdd:cd05936   145 LTHRNLvANALQikaWLED--LLEGDDVVLAALPlfhvFGLTVAL---LLPLALGATIVLiprFRPIG------VLKEIR 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1758 RTGITTLHFVPSMLQQFVQwADADCACD--SLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFwaCQPDD 1835
Cdd:cd05936   214 KHRVTIFPGVPTMYIALLN-APEFKKRDfsSLRLCISGGAPLPVEVAERFEELTGVPIVEGYGLTETSPVVAV--NPLDG 290

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1836 HRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlyKTGDLARWLP 1915
Cdd:cd05936   291 PRKPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNRPEETAEAFVDGWL---------RTGDIGYMDE 361

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1916 DGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAE 1995
Cdd:cd05936   362 DGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEGASLTEEEIIAFCREQLAG 441

                         490       500
                  ....*....|....*....|....*.
gi 641744967 1996 YMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05936   442 YKVPRQVEFRDELPKSAVGKILRREL 467
D-ala-DACP-lig TIGR01734
D-alanine--poly(phosphoribitol) ligase, subunit 1; This model represents the enzyme (also ...
 475-963 2.84e-80

D-alanine--poly(phosphoribitol) ligase, subunit 1; This model represents the enzyme (also called D-alanine-D-alanyl carrier protein ligase) which activates D-alanine as an adenylate via the reaction D-ala + ATP -> D-ala-AMP + PPi, and further catalyzes the condensation of the amino acid adenylate with the D-alanyl carrier protein (D-ala-ACP). The D-alanine is then further transferred to teichoic acid in the biosynthesis of lipoteichoic acid (LTA) and wall teichoic acid (WTA) in gram positive bacteria, both polysacchatides. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273780 [Multi-domain]  Cd Length: 502  Bit Score: 275.48  E-value: 2.84e-80
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   475 IQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMD 554
Cdd:TIGR01734    2 LIEAIQAFAETYPQTIAYRYQGQELTYQQLKEQSDRLAAFIQKRILPKKSPIIVYGHMEPHMLVAFLGSIKSGHAYIPVD 81

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   555 PAYPAERLAYILDDAAPVALLTQSAqvaqlnstLPTVLLDTPAAaacpdTNPVVQGLHAAHLA-------------YVIY 621
Cdd:TIGR01734   82 TSIPSERIEMIIEAAGPELVIHTAE--------LSIDAVGTQII-----TLSALEQAETSGGPvsfdhavkgddnyYIIY 148

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   622 TSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWI-QLLSGHTLVLVPDALRADAHQLWRYF 700
Cdd:TIGR01734  149 TSGSTGNPKGVQISHDNLVSFTNWMLADFPLSEGKQFLNQAPFSFDLSVMDLYpCLASGGTLHCLDKDITNNFKLLFEEL 228

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   701 ARHAVDLFDCTP--VQLQwLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRL-QSLSDTRFINVYGPTECTVDATAC---- 773
Cdd:TIGR01734  229 PKTGLNVWVSTPsfVDMC-LLDPNFNQENYPHLTHFLFCGEELPVKTAKALlERFPKATIYNTYGPTEATVAVTSVkitq 307

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   774 -VVDRTQPLPtIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFipdpFSADpAARIYKTGDL 852
Cdd:TIGR01734  308 eILDQYPRLP-IGFAKPDMNLFIMDEEGEPLPEGEKGEIVIVGPSVSKGYLNNPEKTAEAF----FSHE-GQPAYRTGDA 381

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   853 ARwLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGD-TRLVAYLCARPD----AELHPAAL 927
Cdd:TIGR01734  382 GT-ITDGQLFYQGRLDFQIKLHGYRIELEDIEFNLRQSSYIESAVVVPKYNKDHKvEYLIAAIVPETEdfekEFQLTKAI 460

                          490       500       510
                   ....*....|....*....|....*....|....*.
gi 641744967   928 RQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:TIGR01734  461 KKELKKSLPAYMIPRKFIYRDQLPLTANGKIDRKAL 496
D-ala-DACP-lig TIGR01734
D-alanine--poly(phosphoribitol) ligase, subunit 1; This model represents the enzyme (also ...
 2607-3097 5.29e-80

D-alanine--poly(phosphoribitol) ligase, subunit 1; This model represents the enzyme (also called D-alanine-D-alanyl carrier protein ligase) which activates D-alanine as an adenylate via the reaction D-ala + ATP -> D-ala-AMP + PPi, and further catalyzes the condensation of the amino acid adenylate with the D-alanyl carrier protein (D-ala-ACP). The D-alanine is then further transferred to teichoic acid in the biosynthesis of lipoteichoic acid (LTA) and wall teichoic acid (WTA) in gram positive bacteria, both polysacchatides. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273780 [Multi-domain]  Cd Length: 502  Bit Score: 274.71  E-value: 5.29e-80
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2607 LIHELFeAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPL 2686
Cdd:TIGR01734    2 LIEAIQ-AFAETYPQTIAYRYQGQELTYQQLKEQSDRLAAFIQKRILPKKSPIIVYGHMEPHMLVAFLGSIKSGHAYIPV 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2687 DPTYPVERLRYMLDDAKPVALISQSAhLGIMNGSLPVI---LLDDGETRPFDNEPDTPLDARKQgltprhlAYVIYTSGS 2763
Cdd:TIGR01734   81 DTSIPSERIEMIIEAAGPELVIHTAE-LSIDAVGTQIItlsALEQAETSGGPVSFDHAVKGDDN-------YYIIYTSGS 152

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2764 TGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHA 2843
Cdd:TIGR01734  153 TGNPKGVQISHDNLVSFTNWMLADFPLSEGKQFLNQAPFSFDLSVMDLYPCLASGGTLHCLDKDITNNFKLLFEELPKTG 232

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2844 VSFMQATPSTWRMLVelrdfaLPPGFKA----------LCgGEALPENLATALLQKV--TTLWNLYGPTETTIWSTLNGL 2911
Cdd:TIGR01734  233 LNVWVSTPSFVDMCL------LDPNFNQenyphlthflFC-GEELPVKTAKALLERFpkATIYNTYGPTEATVAVTSVKI 305

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2912 T------TPTPYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITdpFSGAPEarmYKTGD 2985
Cdd:TIGR01734  306 TqeildqYPRLPIGFAKPDMNLFIMDEEGEPLPEGEKGEIVIVGPSVSKGYLNNPEKTAEAFFS--HEGQPA---YRTGD 380

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2986 LGRwLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDsPGDKrlVAYLLAQpdTVLEPAD----- 3060
Cdd:TIGR01734  381 AGT-ITDGQLFYQGRLDFQIKLHGYRIELEDIEFNLRQSSYIESAVVVPKYN-KDHK--VEYLIAA--IVPETEDfekef 454

                          490       500       510       520
                   ....*....|....*....|....*....|....*....|..
gi 641744967  3061 -----LRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:TIGR01734  455 qltkaIKKELKKSLPAYMIPRKFIYRDQLPLTANGKIDRKAL 496
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
 1523-2021 1.05e-77

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 268.59  E-value: 1.05e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1523 DFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAG 1602
Cdd:PRK06187    1 MQDYPLTIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1603 AAYVPLDPGYPAERLAYMLDDASPVALLTQANQRALLTGDVPR-------ILLDTADFSHLSEDNPHV------------ 1663
Cdd:PRK06187   81 AVLHPINIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPQlptvrtvIVEGDGPAAPLAPEVGEYeellaaasdtfd 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1664 -PGLDAHHLAYVIYTSGSTGKPKGVMNSHRAL------CNRlvWMqntyRLTPDDRVLQKTPFsFDVSVWEFFW-PLLYG 1735
Cdd:PRK06187  161 fPDIDENDAAAMLYTSGTTGHPKGVVLSHRNLflhslaVCA--WL----KLSRDDVYLVIVPM-FHVHAWGLPYlALMAG 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1736 ARLVMARpdgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCAcD--SLRRVICSGEALPAELQQRFFARFNAQL 1813
Cdd:PRK06187  234 AKQVIPR---RFDPENLLDLIETERVTFFFAVPTIWQMLLKAPRAYFV-DfsSLRLVIYGGAALPPALLREFKEKFGIDL 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1814 HNLYGPTEAAIDVTFwaCQPDDH---RSFVPI--GRPIANTQLYILDTLGQPVP--LGVAGELHIGGVGVARGYLNRPDL 1886
Cdd:PRK06187  310 VQGYGMTETSPVVSV--LPPEDQlpgQWTKRRsaGRPLPGVEARIVDDDGDELPpdGGEVGEIIVRGPWLMQGYWNRPEA 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1887 TAERFIPDpfinqpgarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDT 1966
Cdd:PRK06187  388 TAETIDGG---------WLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGE 458

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1967 RLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK06187  459 RPVAVVVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVL 513
A_NRPS_alphaAR cd17647
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ...
 1555-2024 1.31e-74

Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.


Pssm-ID: 341302 [Multi-domain]  Cd Length: 520  Bit Score: 259.76  E-value: 1.31e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1555 TYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALltqan 1634
Cdd:cd17647    22 TYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPPARQNIYLGVAKPRGL----- 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1635 qralltgdvprILLDTADFSHLSEDNPHVPgldahhlayviYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVL 1714
Cdd:cd17647    97 -----------IVIRAAGVVVGPDSNPTLS-----------FTSGSEGIPKGVLGRHFSLAYYFPWMAKRFNLSENDKFT 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1715 QKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVqwADADCACDSLRRVICSG 1794
Cdd:cd17647   155 MLSGIAHDPIQRDMFTPLFLGAQLLVPTQDDIGTPGRLAEWMAKYGATVTHLTPAMGQLLT--AQATTPFPKLHHAFFVG 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1795 EALpaelQQRFFARF-----NAQLHNLYGPTEAAIDVTFW---ACQPDDH-----RSFVPIGRPIANTQLYILDTL--GQ 1859
Cdd:cd17647   233 DIL----TKRDCLRLqtlaeNVRIVNMYGTTETQRAVSYFevpSRSSDPTflknlKDVMPAGRGMLNVQLLVVNRNdrTQ 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1860 PVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINQ--------------------PGARLYKTGDLARWLPDGSL 1919
Cdd:cd17647   309 ICGIGEVGEIYVRAGGLAEGYRGLPELNKEKFVNNWFVEPdhwnyldkdnnepwrqfwlgPRDRLYRTGDLGRYLPNGDC 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1920 EYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQP---------------GVTPDPA- 1983
Cdd:cd17647   389 ECCGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRRDKDEEPTLVSYIVPRFdkpddesfaqedvpkEVSTDPIv 468

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967 1984 -----------DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAP 2024
Cdd:cd17647   469 kgligyrklikDIREFLKKRLASYAIPSLIVVLDKLPLNPNGKVDKPKLQFP 520
C_PKS-NRPS cd19532
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 1069-1494 5.34e-74

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHxxxD motif, a few such as Monascus pilosus lovastatin nonaketide synthase MokA have a non-canonical HRxxxD motif in the C-domain and are unable to catalyze amide-bond formation. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380455 [Multi-domain]  Cd Length: 421  Bit Score: 254.30  E-value: 5.34e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1069 PLSFSQQRLWFLAQL--DPAAsqaYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRF--TSIDGQPAQQIdpdtlgF 1144
Cdd:cd19532     3 PMSFGQSRFWFLQQYleDPTT---FNVTFSYRLTGPLDVARLERAVRAVGQRHEALRTCFftDPEDGEPMQGV------L 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1145 SLSSHDLRKLDEAARTTRVAELAEQEARArFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQA 1224
Cdd:cd19532    74 ASSPLRLEHVQISDEAEVEEEFERLKNHV-YDLESGETMRIVLLSLSPTEHYLIFGYHHIAMDGVSFQIFLRDLERAYNG 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1225 AlegseaNLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPA---LLEIPTDRPRPSVQRYAGDQVPFHLDAGQ 1301
Cdd:cd19532   153 Q------PLLPPPLQYLDFAARQRQDYESGALDEDLAYWKSEFSTLPEplpLLPFAKVKSRPPLTRYDTHTAERRLDAAL 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1302 LRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQV 1381
Cdd:cd19532   227 AARIKEASRKLRVTPFHFYLAALQVLLARLLDVDDICIGIADANRTDEDFMETIGFFLNLLPLRFRRDPSQTFADVLKET 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1382 RERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMlsLNNTPAQALTLP----DLTLSAVERPQHSthFDLSLSLIET 1457
Cdd:cd19532   307 RDKAYAALAHSRVPFDVLLDELGVPRSATHSPLFQVF--INYRQGVAESRPfgdcELEGEEFEDARTP--YDLSLDIIDN 382

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|...
gi 641744967 1458 ENGlnGGLVYAT---DLFDRE---TILRvvGYVeNILMAMADD 1494
Cdd:cd19532   383 PDG--DCLLTLKvqsSLYSEEdaeLLLD--SYV-NLLEAFARD 420
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
 1538-2018 2.44e-73

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 252.92  E-value: 2.44e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:cd17631     5 ARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPEV 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLtqanqralltgdvprilldtadfshlsednphvpgldaHHLAYVIYTSGSTGKPKGVMNSHRALCNR 1697
Cdd:cd17631    85 AYILADSGAKVLF--------------------------------------DDLALLMYTSGTTGRPKGAMLTHRNLLWN 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1698 LVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLY-GARLVMARpdgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQ 1776
Cdd:cd17631   127 AVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTLLrGGTVVILR---KFDPETVLDLIERHRVTSFFLVPTMIQALLQ 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1777 ---WADADCAcdSLRRVICSGEALPAELQQRFfARFNAQLHNLYGPTEAAIDVTFwaCQPDDHRS-FVPIGRPIANTQLY 1852
Cdd:cd17631   204 hprFATTDLS--SLRAVIYGGAPMPERLLRAL-QARGVKFVQGYGMTETSPGVTF--LSPEDHRRkLGSAGRPVFFVEVR 278

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1853 ILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKLR 1932
Cdd:cd17631   279 IVDPDGREVPPGEVGEIVVRGPHVMAGYWNRPEATAAAFRDGWF---------HTGDLGRLDEDGYLYIVDRKKDMIISG 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1933 GFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTP 2012
Cdd:cd17631   350 GENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAELDEDELIAHCRERLARYKIPKSVEFVDALPRNA 429


                  ....*.
gi 641744967 2013 NGKLDR 2018
Cdd:cd17631   430 TGKILK 435
DCL_NRPS cd19543
DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the ...
 2161-2567 8.92e-72

DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor; The DCL-type Condensation (C) domain catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. This domain is D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains in addition to the LCL- and DCL-types such as starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380465 [Multi-domain]  Cd Length: 423  Bit Score: 248.27  E-value: 8.92e-72
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2161 IYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILPIN 2240
Cdd:cd19543     1 IYPLSPMQEGMLFHSLLDPGSGAYVEQMVITLEGPLDPDRFRAAWQAVVDRHPILRTSFVWEGLGEPLQVVLKDRKLPWR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2241 HFEPT--SPEDVLAQLQAH-TEPRTRRIDLSQAPLFRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEI----RLLL 2313
Cdd:cd19543    81 ELDLShlSEAEQEAELEALaEEDRERGFDLARAPLMRLTLIRLG-DDRYRLVWSFHHILLDGWSLPILLKELfaiyAALG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2314 QHQADALPTPLPYRNFIAQTLSVPNSAHEAYFRDKLADVDEPTA-PFGLLNVQGSGGDIHEARLVLDATLASAIRQQARH 2392
Cdd:cd19543   160 EGQPPSLPPVRPYRDYIAWLQRQDKEAAEAYWREYLAGFEEPTPlPKELPADADGSYEPGEVSFELSAELTARLQELARQ 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2393 LGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADRIMGMFINTLPLRISL-ADRGAAEVVERTSHDLMTLL 2471
Cdd:cd19543   240 HGVTLNTVVQGAWALLLSRYSGRDDVVFGTTVSGRPAELPGIETMVGLFINTLPVRVRLdPDQTVLELLKDLQAQQLELR 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2472 EHEQAPLALAQRCSgvAPPMPLFSTLL---NYRHTQASSTDNTLSDIRV--LTSEERTNYPLTLAVdDRGEGFSLV---- 2542
Cdd:cd19543   320 EHEYVPLYEIQAWS--EGKQALFDHLLvfeNYPVDESLEEEQDEDGLRItdVSAEEQTNYPLTVVA-IPGEELTIKlsyd 396

                         410       420
                  ....*....|....*....|....*
gi 641744967 2543 AQTLEDIDPHRLLNYLMTAISSLVD 2567
Cdd:cd19543   397 AEVFDEATIERLLGHLRRVLEQVAA 421
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
 468-963 1.26e-71

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 250.87  E-value: 1.26e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  468 DFPREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAG 547
Cdd:PRK06187    1 MQDYPLTIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  548 AAYVPMDPAYPAERLAYILDDAAPVALLTQS---AQVAQLNSTLPTV----LLDTPAAAACPDTNPVVQGLHAAH----- 615
Cdd:PRK06187   81 AVLHPINIRLKPEEIAYILNDAEDRVVLVDSefvPLLAAILPQLPTVrtviVEGDGPAAPLAPEVGEYEELLAAAsdtfd 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  616 --------LAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALdHPSRIALNASIVFDASVKNW--IQLLSGHTLVLv 685
Cdd:PRK06187  161 fpdidendAAAMLYTSGTTGHPKGVVLSHRNLFLHSLAVCAWLKL-SRDDVYLVIVPMFHVHAWGLpyLALMAGAKQVI- 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  686 PDalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQP-AQVLIGGEAISPAVWSRLQSLSDTRFINVYGPT 764
Cdd:PRK06187  239 PR--RFDPENLLDLIETERVTFFFAVPTIWQMLLKAPRAYFVDFSSlRLVIYGGAALPPALLREFKEKFGIDLVQGYGMT 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  765 ECTVDATAC-----VVDRTQPLPTIGKPLANTRLYILDAQDQPVP--IGVTGELHIGGAGVARGYLHRPDLTAERFIPDp 837
Cdd:PRK06187  317 ETSPVVSVLppedqLPGQWTKRRSAGRPLPGVEARIVDDDGDELPpdGGEVGEIIVRGPWLMQGYWNRPEATAETIDGG- 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  838 fsadpaarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCAR 917
Cdd:PRK06187  396 --------WLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLK 467

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967  918 PDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK06187  468 PGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVL 513
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
 1530-2021 8.74e-71

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 248.28  E-value: 8.74e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLD 1609
Cdd:PRK07656    7 LPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLN 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1610 PGYPAERLAYMLDDASPVALLTQAnqrALLTGDVPR----------ILLDT-------------ADFSHLSEDNPHVPGL 1666
Cdd:PRK07656   87 TRYTADEAAYILARGDAKALFVLG---LFLGVDYSAttrlpalehvVICETeeddphtekmktfTDFLAAGDPAERAPEV 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1667 DAHHLAYVIYTSGSTGKPKGVMNSHRalcnrlvwmqNTYR----------LTPDDRVLQKTPFsFDVSVWEFFW--PLLY 1734
Cdd:PRK07656  164 DPDDVADILFTSGTTGRPKGAMLTHR----------QLLSnaadwaeylgLTEGDRYLAANPF-FHVFGYKAGVnaPLMR 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1735 GARLVMARpdgHKDAAYLAQLIERTGITTLHFVPSMLQ---QFVQWADADCAcdSLRRVICSGEALPAELQQRFFARFNA 1811
Cdd:PRK07656  233 GATILPLP---VFDPDEVFRLIETERITVLPGPPTMYNsllQHPDRSAEDLS--SLRLAVTGAASMPVALLERFESELGV 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1812 Q-LHNLYGPTEAAIDVTFwaCQPDDHRSFVP--IGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTA 1888
Cdd:PRK07656  308 DiVLTGYGLSEASGVTTF--NRLDDDRKTVAgtIGTAIAGVENKIVNELGEEVPVGEVGELLVRGPNVMKGYYDDPEATA 385

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1889 ERFIPDPFinqpgarLYkTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVaredspG--DT 1966
Cdd:PRK07656  386 AAIDADGW-------LH-TGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVI------GvpDE 451

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 1967 RL----VAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK07656  452 RLgevgKAYVVLKPGAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRAL 510
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
483-963 3.30e-70

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 248.49  E-value: 3.30e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFED-----QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAY 557
Cdd:COG0365    19 AEGRGDKVALIWEGedgeeRTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSPVFPGF 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  558 PAERLAYILDDAAPVALLTQSAQ------------VAQLNSTLPTV----LLDTP--------------AAAACPDTNPV 607
Cdd:COG0365    99 GAEALADRIEDAEAKVLITADGGlrggkvidlkekVDEALEELPSLehviVVGRTgadvpmegdldwdeLLAAASAEFEP 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  608 VQgLHAAHLAYVIYTSGSTGRPKGVMVAHRNVInLATGLHTLLALD-HPSRIALNASIVFdasvknWI---------QLL 677
Cdd:COG0365   179 EP-TDADDPLFILYTSGTTGKPKGVVHTHGGYL-VHAATTAKYVLDlKPGDVFWCTADIG------WAtghsyivygPLL 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  678 SGHTLVLVPDALRA-DAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQV-LIG--GEAISPAVWSRLQSLS 753
Cdd:COG0365   251 NGATVVLYEGRPDFpDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSLrLLGsaGEPLNPEVWEWWYEAV 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  754 DTRFINVYGPTECTvdatACVVdrtQPLP-------TIGKPLANTRLYILDAQDQPVPIGVTGELHIGGA--GVARGYLH 824
Cdd:COG0365   331 GVPIVDGWGQTETG----GIFI---SNLPglpvkpgSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGPwpGMFRGYWN 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  825 RPDLTAERFipdpFSADPaaRIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDS 904
Cdd:COG0365   404 DPERYRETY----FGRFP--GWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDE 477

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967  905 PGDTRLVAYLCARPDAELHPA---ALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:COG0365   478 IRGQVVKAFVVLKPGVEPSDElakELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLL 539
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
 479-963 1.19e-69

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 243.62  E-value: 1.19e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  479 FEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:cd05936     5 LEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPLYT 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTqsaqvaqlNSTLPTVLldtpaaaACPDTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRN 638
Cdd:cd05936    85 PRELEHILNDSGAKALIV--------AVSFTDLL-------AAGAPLGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRN 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  639 -VINLATGLHTLLALDHPSRIALNASIVFDA---SVKNWIQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQ 714
Cdd:cd05936   150 lVANALQIKAWLEDLLEGDDVVLAALPLFHVfglTVALLLPLALGATIVLIP---RFRPIGVLKEIRKHRVTIFPGVPTM 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  715 LQWLLDAglGSDPAYQPAQVLI---GGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANT 791
Cdd:cd05936   227 YIALLNA--PEFKKRDFSSLRLcisGGAPLPVEVAERFEELTGVPIVEGYGLTETSPVVAVNPLDGPRKPGSIGIPLPGT 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  792 RLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPDGNIDYLGRNDFQI 871
Cdd:cd05936   305 EVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNRPEETAEAFVDGWL---------RTGDIGYMDEDGYFFIVDRKKDMI 375

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  872 KVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALP 951
Cdd:cd05936   376 IVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEGASLTEEEIIAFCREQLAGYKVPRQVEFRDELP 455

                         490
                  ....*....|..
gi 641744967  952 LTPNGKLDRKAL 963
Cdd:cd05936   456 KSAVGKILRREL 467
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
 615-959 2.52e-69

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 237.95  E-value: 2.52e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  615 HLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIaLNASIVFDASVKNWIQ--LLSGHTLVLVPdalRAD 692
Cdd:cd04433     1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVF-LSTLPLFHIGGLFGLLgaLLAGGTVVLLP---KFD 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  693 AHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLI-GGEAISPAVWSRLQSLSDTRFINVYGPTECTVDAT 771
Cdd:cd04433    77 PEAALELIEREKVTILLGVPTLLARLLKAPESAGYDLSSLRALVsGGAPLPPELLERFEEAPGIKLVNGYGLTETGGTVA 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  772 ACVVDRTQPLP-TIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAErFIPDPFsadpaariYKTG 850
Cdd:cd04433   157 TGPPDDDARKPgSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAA-VDEDGW--------YRTG 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  851 DLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQ 930
Cdd:cd04433   228 DLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAEELRAH 307

                         330       340
                  ....*....|....*....|....*....
gi 641744967  931 LAASLADYMIPSAFVTLDALPLTPNGKLD 959
Cdd:cd04433   308 VRERLAPYKVPRRVVFVDALPRTASGKID 336
alpha_am_amid TIGR03443
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ...
 475-1048 2.18e-68

L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.


Pssm-ID: 274582 [Multi-domain]  Cd Length: 1389  Bit Score: 256.53  E-value: 2.18e-68
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   475 IQQRFEAQAEQTPEAIAVLFEDQHL---------TYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILK 545
Cdd:TIGR03443  238 IHDIFADNAEKHPDRTCVVETPSFLdpssktrsfTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLK 317

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   546 AGAAYVPMDPAYPAERLAYILDDAAPVAL--LTQSAQVAQ-----------LNSTLPTVLL------------------- 593
Cdd:TIGR03443  318 AGATFSVIDPAYPPARQTIYLSVAKPRALivIEKAGTLDQlvrdyidkeleLRTEIPALALqddgslvggsleggetdvl 397

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   594 -------DTPAAAAC-PDTNPVVQglhaahlayviYTSGSTGRPKGVMvahrnvinlatGLHTLLA-----------LDH 654
Cdd:TIGR03443  398 apyqalkDTPTGVVVgPDSNPTLS-----------FTSGSEGIPKGVL-----------GRHFSLAyyfpwmakrfgLSE 455

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   655 PSRIALNASIVFDAsvknwIQ------LLSGHTLvLVPDAlrAD---AHQLWRYFARHAVDLFDCTPVQLQwLLDAGLGS 725
Cdd:TIGR03443  456 NDKFTMLSGIAHDP-----IQrdmftpLFLGAQL-LVPTA--DDigtPGRLAEWMAKYGATVTHLTPAMGQ-LLSAQATT 526

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   726 D-PAYQPAqvLIGGEAISPAVWSRLQSLSDTRFI-NVYGPTEC----------TVDATACVVDRTQPLPTIGKPLANTRL 793
Cdd:TIGR03443  527 PiPSLHHA--FFVGDILTKRDCLRLQTLAENVCIvNMYGTTETqravsyfeipSRSSDSTFLKNLKDVMPAGKGMKNVQL 604

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   794 YILDAQD--QPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPD--------------------PFSADPAARIYKTGD 851
Cdd:TIGR03443  605 LVVNRNDrtQTCGVGEVGEIYVRAGGLAEGYLGLPELNAEKFVNNwfvdpshwidldkennkperEFWLGPRDRLYRTGD 684

                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   852 LARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPD-AELHPAA---- 926
Cdd:TIGR03443  685 LGRYLPDGNVECCGRADDQVKIRGFRIELGEIDTHLSQHPLVRENVTLVRRDKDEEPTLVSYIVPQDKsDELEEFKsevd 764

                          570       580       590       600       610       620       630       640
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   927 ---------------------LRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPD--QTAFATRDY-----EA 978
Cdd:TIGR03443  765 deessdpvvkglikyrklikdIREYLKKKLPSYAIPTVIVPLKKLPLNPNGKVDKPALPFPDtaQLAAVAKNRsasaaDE 844

                          650       660       670       680       690       700       710
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967   979 PQGGIETALAALWQELL--GLDRVGRHDQFFALGGHSLLAVQLLNRMNKAGM-DVALATLFAHPTLCDLAAAV 1048
Cdd:TIGR03443  845 EFTETEREIRDLWLELLpnRPATISPDDSFFDLGGHSILATRMIFELRKKLNvELPLGLIFKSPTIKGFAKEV 917
C_PKS-NRPS_PksJ-like cd20484
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 1069-1494 9.51e-68

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs), similar to Bacillus subtilis PksJ; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily have the typical C-domain HHxxxD motif. PksJ is involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is important in secondary metabolism. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380472 [Multi-domain]  Cd Length: 430  Bit Score: 236.83  E-value: 9.51e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1069 PLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDtLGFSLSS 1148
Cdd:cd20484     3 PLSEGQKGLWMLQKMSPEMS-AYNVPLCFRFSSKLDVEKFKQACQFVLEQHPILKSVIEEEDGVPFQKIEPS-KPLSFQE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1149 HDLRKLDEAarttRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEG 1228
Cdd:cd20484    81 EDISSLKES----EIIAYLREKAKEPFVLENGPLMRVHLFSRSEQEHFVLITIHHIIFDGSSSLTLIHSLLDAYQALLQG 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1229 SEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHAL 1308
Cdd:cd20484   157 KQPTLASSPASYYDFVAWEQDMLAGAEGEEHRAYWKQQLSGTLPILELPADRPRSSAPSFEGQTYTRRLPSELSNQIKSF 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1309 NRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALDA 1388
Cdd:cd20484   237 ARSQSINLSTVFLGIFKLLLHRYTGQEDIIVGMPTMGRPEERFDSLIGYFINMLPIRSRILGEETFSDFIRKLQLTVLDG 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1389 YAHQALPFEQVVEILQPARSLSYSPIFQVMLS----LNNTPAQALTLP---DLTLSAVERPQHSTHFDLSLSLIETENGL 1461
Cdd:cd20484   317 LDHAAYPFPAMVRDLNIPRSQANSPVFQVAFFyqnfLQSTSLQQFLAEyqdVLSIEFVEGIHQEGEYELVLEVYEQEDRF 396

                         410       420       430
                  ....*....|....*....|....*....|...
gi 641744967 1462 NGGLVYATDLFDRETILRVVGYVENILMAMADD 1494
Cdd:cd20484   397 TLNIKYNPDLFDASTIERMMEHYVKLAEELIAN 429
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
 2610-3097 1.21e-67

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 237.85  E-value: 1.21e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2610 ELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPT 2689
Cdd:cd05936     3 DLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2690 YPVERLRYMLDDAKPVALISqsahlgimngslpvillddgeTRPFDN--EPDTPLDaRKQGLTPRHLAYVIYTSGSTGKP 2767
Cdd:cd05936    83 YTPRELEHILNDSGAKALIV---------------------AVSFTDllAAGAPLG-ERVALTPEDVAVLQYTSGTTGVP 140

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2768 KGVMVEHANMV-NFL-CSMRKEPGIAQEDVLLGVTSL--SFDISIlEIFLPLLNGARLILATQAQAADAQQlamLIERHA 2843
Cdd:cd05936   141 KGAMLTHRNLVaNALqIKAWLEDLLEGDDVVLAALPLfhVFGLTV-ALLLPLALGATIVLIPRFRPIGVLK---EIRKHR 216

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2844 VSFMQATPSTWRMLVELRDF--ALPPGFK-ALCGGEALPENLATALLQKV-TTLWNLYGPTETTIWSTLNGLTTPTP--Y 2917
Cdd:cd05936   217 VTIFPGVPTMYIALLNAPEFkkRDFSSLRlCISGGAPLPVEVAERFEELTgVPIVEGYGLTETSPVVAVNPLDGPRKpgS 296

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFiTDPFsgapearmYKTGDLGRWLPDGTLEY 2997
Cdd:cd05936   297 IGIPLPGTEVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNRPEETAEAF-VDGW--------LRTGDIGYMDEDGYFFI 367

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2998 LGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSA 3077
Cdd:cd05936   368 VDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEGASLTEEEIIAFCREQLAGYKVPRQ 447

                         490       500
                  ....*....|....*....|
gi 641744967 3078 FVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05936   448 VEFRDELPKSAVGKILRREL 467
A_NRPS_alphaAR cd17647
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ...
 2631-3100 1.29e-66

Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.


Pssm-ID: 341302 [Multi-domain]  Cd Length: 520  Bit Score: 236.65  E-value: 1.29e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQ 2710
Cdd:cd17647    20 SFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPPARQNIYLGVAKPRGLIVI 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2711 SAhLGIMNGslpvillddgetrpfdnePDTpldarkqglTPRhlayVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGI 2790
Cdd:cd17647   100 RA-AGVVVG------------------PDS---------NPT----LSFTSGSEGIPKGVLGRHFSLAYYFPWMAKRFNL 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2791 AQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELRDFALPPGFK 2870
Cdd:cd17647   148 SENDKFTMLSGIAHDPIQRDMFTPLFLGAQLLVPTQDDIGTPGRLAEWMAKYGATVTHLTPAMGQLLTAQATTPFPKLHH 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2871 ALCGGEALPEN--LATALLQKVTTLWNLYGPTETT-------IWST------LNGLTTPTPyIGHPIANTQIYILDAQGR 2935
Cdd:cd17647   228 AFFVGDILTKRdcLRLQTLAENVRIVNMYGTTETQravsyfeVPSRssdptfLKNLKDVMP-AGRGMLNVQLLVVNRNDR 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2936 --VVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITD--------------------PFSGAPEARMYKTGDLGRWLPDG 2993
Cdd:cd17647   307 tqICGIGEVGEIYVRAGGLAEGYRGLPELNKEKFVNNwfvepdhwnyldkdnnepwrQFWLGPRDRLYRTGDLGRYLPNG 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2994 TLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPD--------TVLEPA------ 3059
Cdd:cd17647   387 DCECCGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRRDKDEEPTLVSYIVPRFDkpddesfaQEDVPKevstdp 466

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....
gi 641744967 3060 -------------DLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAP 3100
Cdd:cd17647   467 ivkgligyrklikDIREFLKKRLASYAIPSLIVVLDKLPLNPNGKVDKPKLQFP 520
A_NRPS_acs4 cd17654
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ...
 2620-3097 6.05e-66

acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341309 [Multi-domain]  Cd Length: 449  Bit Score: 232.36  E-value: 6.05e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGE----ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERL 2695
Cdd:cd17654     1 PDRPALIIDQttsdTTVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAAYAPIDPASPEQRS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2696 RYMLDDAKpVALISQSAHlgimNGSLPVILLDdgETRPFDNepdtpldarkqgLTPRHLAYVIYTSGSTGKPKGVMVEHA 2775
Cdd:cd17654    81 LTVMKKCH-VSYLLQNKE----LDNAPLSFTP--EHRHFNI------------RTDECLAYVIHTSGTTGTPKIVAVPHK 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2776 NMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILA-TQAQAADAQQLAMLIERHAVSFMQATPSTW 2854
Cdd:cd17654   142 CILPNIQHFRSLFNITSEDILFLTSPLTFDPSVVEIFLSLSSGATLLIVpTSVKVLPSKLADILFKRHRITVLQATPTLF 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2855 RML-VELRDFALPPGFKAL----CGGEALPENLATALLQKV---TTLWNLYGPTETTIWSTLN---GLTTPTPyIGHPIA 2923
Cdd:cd17654   222 RRFgSQSIKSTVLSATSSLrvlaLGGEPFPSLVILSSWRGKgnrTRIFNIYGITEVSCWALAYkvpEEDSPVQ-LGSPLL 300

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2924 NTQIYILDAQGRvvplGVAGEIHIagAGVVRGYlgrpdltaerfITDPFSGAPEARMYKTGDLGRwLPDGTLEYLGRNDF 3003
Cdd:cd17654   301 GTVIEVRDQNGS----EGTGQVFL--GGLNRVC-----------ILDDEVTVPKGTMRATGDFVT-VKDGELFFLGRKDS 362

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3004 QVKVRGFRIELGEIETRLARCHGVhDAVVIAREDspgDKRLVAYLLAQPDTVLEPADLRQRLsegVAEYMIPSAFVTLDA 3083
Cdd:cd17654   363 QIKRRGKRINLDLIQQVIESCLGV-ESCAVTLSD---QQRLIAFIVGESSSSRIHKELQLTL---LSSHAIPDTFVQIDK 435

                         490
                  ....*....|....
gi 641744967 3084 FPLTPNGKLDRKAL 3097
Cdd:cd17654   436 LPLTSHGKVDKSEL 449
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
 2753-3093 2.06e-65

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 226.40  E-value: 2.06e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2753 HLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADA 2832
Cdd:cd04433     1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPKFDPEAA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2833 QqlaMLIERHAVSFMQATPSTWRMLVEL---RDFALPPGFKALCGGEALPENLATALLQKV-TTLWNLYGPTETTIWSTL 2908
Cdd:cd04433    81 L---ELIEREKVTILLGVPTLLARLLKApesAGYDLSSLRALVSGGAPLPPELLERFEEAPgIKLVNGYGLTETGGTVAT 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 NGLTTPT---PYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFitdpfsgapEARMYKTGD 2985
Cdd:cd04433   158 GPPDDDArkpGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAAVD---------EDGWYRTGD 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2986 LGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRL 3065
Cdd:cd04433   229 LGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAEELRAHV 308

                         330       340
                  ....*....|....*....|....*...
gi 641744967 3066 SEGVAEYMIPSAFVTLDAFPLTPNGKLD 3093
Cdd:cd04433   309 RERLAPYKVPRRVVFVDALPRTASGKID 336
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
 1550-2016 2.23e-65

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 231.72  E-value: 2.23e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1550 EDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVAL 1629
Cdd:cd05911     7 TGKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVI 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1630 LTQAN-----QRAL-LTGDVPRI-LLDTADFSHLS-------------EDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMN 1689
Cdd:cd05911    87 FTDPDglekvKEAAkELGPKDKIiVLDDKPDGVLSiedllsptlgeedEDLPPPLKDGKDDTAAILYSSGTTGLPKGVCL 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRL--VWMQNTYRLTPDDRVLQKTPFsFDVS-VWEFFWPLLYGARLV-MARPdghkDAAYLAQLIERTGITTLH 1765
Cdd:cd05911   167 SHRNLIANLsqVQTFLYGNDGSNDVILGFLPL-YHIYgLFTTLASLLNGATVIiMPKF----DSELFLDLIEKYKITFLY 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1766 FVPSMLQQFVQWADADCA-CDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEaaidVTFWACQPDDHRSFVP-I 1842
Cdd:cd05911   242 LVPPIAAALAKSPLLDKYdLSSLRVILSGGAPLSKELQELLAKRFpNATIKQGYGMTE----TGGILTVNPDGDDKPGsV 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1843 GRPIANTQLYILDTLG-QPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEY 1921
Cdd:cd05911   318 GRLLPNVEAKIVDDDGkDSLGPNEPGEICVRGPQVMKGYYNNPEATKETFDEDGW--------LHTGDIGYFDEDGYLYI 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1922 LGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEY--MVP 1999
Cdd:cd05911   390 VDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEKLTEKEVKDYVAKKVASYkqLRG 469

                         490
                  ....*....|....*..
gi 641744967 2000 GAFVTlDAFPLTPNGKL 2016
Cdd:cd05911   470 GVVFV-DEIPKSASGKI 485
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
 1554-2021 2.51e-65

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 231.82  E-value: 2.51e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA 1633
Cdd:cd05926    15 LTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLTPK 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 N-----QRALLTgDVPRIL---LDTADFsHLSEDNPHVPGLDAHH-------------LAYVIYTSGSTGKPKGVMNSHR 1692
Cdd:cd05926    95 GelgpaSRAASK-LGLAILelaLDVGVL-IRAPSAESLSNLLADKknaksegvplpddLALILHTSGTTGRPKGVPLTHR 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1693 ALCNRLVWMQNTYRLTPDDRVLQKTPFsFDVS--VWEFFWPLLYGARLVMarPDGHkDAAYLAQLIERTGITTLHFVPSM 1770
Cdd:cd05926   173 NLAASATNITNTYKLTPDDRTLVVMPL-FHVHglVASLLSTLAAGGSVVL--PPRF-SASTFWPDVRDYNATWYTAVPTI 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1771 LQQFVQWADADC--ACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTfwaCQP--DDHRSFVPIGRPi 1846
Cdd:cd05926   249 HQILLNRPEPNPesPPPKLRFIRSCSASLPPAVLEALEATFGAPVLEAYGMTEAAHQMT---SNPlpPGPRKPGSVGKP- 324

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1847 ANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGRND 1926
Cdd:cd05926   325 VGVEVRILDEDGEILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGW--------FRTGDLGYLDADGYLFLTGRIK 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1927 FQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLD 2006
Cdd:cd05926   397 ELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASVTEEELRAFCRKHLAAFKVPKKVYFVD 476

                         490
                  ....*....|....*
gi 641744967 2007 AFPLTPNGKLDRKAL 2021
Cdd:cd05926   477 ELPKTATGKIQRRKV 491
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 496-964 3.87e-65

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 228.72  E-value: 3.87e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  496 DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALL 575
Cdd:cd05934     1 GRRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  576 TQsaqvaqlnstlptvlldtpaaaacpdtnpvvqglhaahLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALdHP 655
Cdd:cd05934    81 VD--------------------------------------PASILYTSGTTGPPKGVVITHANLTFAGYYSARRFGL-GE 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  656 SRIALNASIVF--DASVKNWIQ-LLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPA 732
Cdd:cd05934   122 DDVYLTVLPLFhiNAQAVSVLAaLSVGATLVLLP---RFSASRFWSDVRRYGATVTNYLGAMLSYLLAQPPSPDDRAHRL 198

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  733 QVlIGGEAISPAVWSRLQSLSDTRFINVYGPTEcTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELH 812
Cdd:cd05934   199 RA-AYGAPNPPELHEEFEERFGVRLLEGYGMTE-TIVGVIGPRDEPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELV 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  813 I---GGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLR 889
Cdd:cd05934   277 IrglRGWGFFKGYYNMPEATAEAM---------RNGWFHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILR 347

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 641744967  890 CPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALP 964
Cdd:cd05934   348 HPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
 483-960 1.76e-63

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 224.41  E-value: 1.76e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:cd17631     5 ARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPEV 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPvalltqsaqvaqlnstlpTVLLDTPAaaacpdtnpvvqglhaahlaYVIYTSGSTGRPKGVMVAHRNVINL 642
Cdd:cd17631    85 AYILADSGA------------------KVLFDDLA--------------------LLMYTSGTTGRPKGAMLTHRNLLWN 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  643 ATGLHTLLALDHPSRIALNASI--VFDASVKNWIQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLD 720
Cdd:cd17631   127 AVNALAALDLGPDDVLLVVAPLfhIGGLGVFTLPTLLRGGTVVILR---KFDPETVLDLIERHRVTSFFLVPTMIQALLQ 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  721 AGLGSDPAYQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLP-TIGKPLANTRLYILDAQ 799
Cdd:cd17631   204 HPRFATTDLSSLRAVIYGGAPMPERLLRALQARGVKFVQGYGMTETSPGVTFLSPEDHRRKLgSAGRPVFFVEVRIVDPD 283

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  800 DQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIE 879
Cdd:cd17631   284 GREVPPGEVGEIVVRGPHVMAGYWNRPEATAAAFRDGWF---------HTGDLGRLDEDGYLYIVDRKKDMIISGGENVY 354

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  880 AGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLD 959
Cdd:cd17631   355 PAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAELDEDELIAHCRERLARYKIPKSVEFVDALPRNATGKIL 434


                  .
gi 641744967  960 R 960
Cdd:cd17631   435 K 435
ligase_PEP_1 TIGR03098
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ...
 480-965 1.13e-61

acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.


Pssm-ID: 211788 [Multi-domain]  Cd Length: 517  Bit Score: 221.96  E-value: 1.13e-61
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   480 EAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPA 559
Cdd:TIGR03098    7 EDAAARLPDATALVHHDRTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKA 86

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   560 ERLAYILDDAAPVALLTQSAQVAQLNSTLPT-------VLLDTPA----------------AAACPDTNPVVQGLhAAHL 616
Cdd:TIGR03098   87 EQVAHILADCNVRLLVTSSERLDLLHPALPGchdlrtlIIVGDPAhaseghpgeepaswpkLLALGDADPPHPVI-DSDM 165

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   617 AYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKnwiQLLS----GHTLVLVPDALRAD 692
Cdd:TIGR03098  166 AAILYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYGFN---QLTTafyvGATVVLHDYLLPRD 242

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   693 ahqLWRYFARHAVDLFDCTP------VQLQWLLDAGlgsdpayQPAQVLIG-GEAISPAVWSRLQS-LSDTRFINVYGPT 764
Cdd:TIGR03098  243 ---VLKALEKHGITGLAAVPplwaqlAQLDWPESAA-------PSLRYLTNsGGAMPRATLSRLRSfLPNARLFLMYGLT 312

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   765 EcTVDATACVVDRTQPLPT-IGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSAD-- 841
Cdd:TIGR03098  313 E-AFRSTYLPPEEVDRRPDsIGKAIPNAEVLVLREDGSECAPGEEGELVHRGALVAMGYWNDPEKTAERFRPLPPFPGel 391

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   842 --PAARIYkTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPD 919
Cdd:TIGR03098  392 hlPELAVW-SGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTLGQAIVLVVTPPGG 470

                          490       500       510       520
                   ....*....|....*....|....*....|....*....|....*.
gi 641744967   920 AELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPA 965
Cdd:TIGR03098  471 EELDRAALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
DCL_NRPS cd19543
DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the ...
 28-399 3.86e-61

DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor; The DCL-type Condensation (C) domain catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. This domain is D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains in addition to the LCL- and DCL-types such as starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380465 [Multi-domain]  Cd Length: 423  Bit Score: 217.46  E-value: 3.86e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   28 IYPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHQPVQVVWRQAPLTVN 107
Cdd:cd19543     1 IYPLSPMQEGMLFHSLLDPGSGAYVEQMVITLEGPLDPDRFRAAWQAVVDRHPILRTSFVWEGLGEPLQVVLKDRKLPWR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  108 T--LTTTSSDTVPAQLRA-ATDPSNHRLNLSNAPLLSAT---TAHDpvcgEWLLSLSIHHLISDHITQALIIDEI----R 177
Cdd:cd19543    81 EldLSHLSEAEQEAELEAlAEEDRERGFDLARAPLMRLTlirLGDD----RYRLVWSFHHILLDGWSLPILLKELfaiyA 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  178 LLLEDRPEALPKPLPYRNFIAQILSVPLSEHEQYFRNRLADIDTPTA-PFDLVDVQGNGEDITEARLSLDSSLADALRRQ 256
Cdd:cd19543   157 ALGEGQPPSLPPVRPYRDYIAWLQRQDKEAAEAYWREYLAGFEEPTPlPKELPADADGSYEPGEVSFELSAELTARLQEL 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  257 ARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGADRVMGMFINTLPLRVSLRER-SVHDVVQATSHELM 335
Cdd:cd19543   237 ARQHGVTLNTVVQGAWALLLSRYSGRDDVVFGTTVSGRPAELPGIETMVGLFINTLPVRVRLDPDqTVLELLKDLQAQQL 316

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967  336 MLLAHEQAPLALAQQCSQVPPplPLFSTLF---NYRHSQKDASSQFWEG--MRQLSGRERTNYPITLSV 399
Cdd:cd19543   317 ELREHEYVPLYEIQAWSEGKQ--ALFDHLLvfeNYPVDESLEEEQDEDGlrITDVSAEEQTNYPLTVVA 383
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
 2607-3097 3.94e-61

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 220.44  E-value: 3.94e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2607 LIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPL 2686
Cdd:PRK06187    7 TIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPI 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2687 DPTYPVERLRYMLDDAKPVALISQSAHLGIMNGSLP-------VILLDDGETRPF-----------DNEPDTPLDARkqg 2748
Cdd:PRK06187   87 NIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPqlptvrtVIVEGDGPAAPLapevgeyeellAAASDTFDFPD--- 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2749 LTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLsFDISILEI-FLPLLNGARLILATQA 2827
Cdd:PRK06187  164 IDENDAAAMLYTSGTTGHPKGVVLSHRNLFLHSLAVCAWLKLSRDDVYLVIVPM-FHVHAWGLpYLALMAGAKQVIPRRF 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2828 QAADAQQlamLIERHAVSFMQATPSTWRMLVElrdfALPPGFK-------ALCGGEALPENLATALLQKV-TTLWNLYGP 2899
Cdd:PRK06187  243 DPENLLD---LIETERVTFFFAVPTIWQMLLK----APRAYFVdfsslrlVIYGGAALPPALLREFKEKFgIDLVQGYGM 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2900 TETTIWSTLN----GLTTPTPYI---GHPIANTQIYILDAQGRVVP--LGVAGEIHIAGAGVVRGYLGRPDLTAERFITD 2970
Cdd:PRK06187  316 TETSPVVSVLppedQLPGQWTKRrsaGRPLPGVEARIVDDDGDELPpdGGEVGEIIVRGPWLMQGYWNRPEATAETIDGG 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2971 pfsgapearMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLA 3050
Cdd:PRK06187  396 ---------WLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVL 466

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*..
gi 641744967 3051 QPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK06187  467 KPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVL 513
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
 1543-2021 4.26e-61

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 217.93  E-value: 4.26e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1543 DTTAVLFEDQHLTYDALNRRANQLAHHLIDLG-VKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:cd05941     1 DRIAIVDDGDSITYADLVARAARLANRLLALGkDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPvalltqanqralltgdvpRILLDtadfshlsednphvpgldahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM 1701
Cdd:cd05941    81 TDSEP------------------SLVLD---------------------PALILYTSGTTGRPKGVVLTHANLAANVRAL 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFsFDVS--VWEFFWPLLYGARLVMARPDghkDAAYLAQLIERTGITTLHFVPSMLQQFVQWAD 1779
Cdd:cd05941   122 VDAWRWTEDDVLLHVLPL-HHVHglVNALLCPLFAGASVEFLPKF---DPKEVAISRLMPSITVFMGVPTIYTRLLQYYE 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1780 ADC---------ACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTfwaCQPDDHRSFVPIGRPIANTQ 1850
Cdd:cd05941   198 AHFtdpqfaraaAAERLRLMVSGSAALPVPTLEEWEAITGHTLLERYGMTEIGMALS---NPLDGERRPGTVGMPLPGVQ 274

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1851 LYILDTL-GQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGR-NDFQ 1928
Cdd:cd05941   275 ARIVDEEtGEPLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDDGW--------FKTGDLGVVDEDGYYWILGRsSVDI 346

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1929 VKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTP-DPADLRQQLGQHLAEYMVPGAFVTLDA 2007
Cdd:cd05941   347 IKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGAAAlSLEELKEWAKQRLAPYKRPRRLILVDE 426

                         490
                  ....*....|....
gi 641744967 2008 FPLTPNGKLDRKAL 2021
Cdd:cd05941   427 LPRNAMGKVNKKEL 440
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1551-2022 4.36e-61

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 217.16  E-value: 4.36e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1551 DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALL 1630
Cdd:cd05934     1 GRRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1631 TQanqralltgdvprilldtadfshlsednphvpgldahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPD 1710
Cdd:cd05934    81 VD--------------------------------------PASILYTSGTTGPPKGVVITHANLTFAGYYSARRFGLGED 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1711 DRVLQKTP-FSFDVSVWEFFWPLLYGARLVMARPdgHKDAAYLAQlIERTGITTLHFVPSMLQQFVQWADADCACDSLRR 1789
Cdd:cd05934   123 DVYLTVLPlFHINAQAVSVLAALSVGATLVLLPR--FSASRFWSD-VRRYGATVTNYLGAMLSYLLAQPPSPDDRAHRLR 199

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1790 VICSGEAlPAELQQRFFARFNAQLHNLYGPTEAAIDVtfwACQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGEL 1869
Cdd:cd05934   200 AAYGAPN-PPELHEEFEERFGVRLLEGYGMTETIVGV---IGPRDEPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGEL 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1870 HIGGV---GVARGYLNRPDLTAERFiPDPFinqpgarlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLM 1946
Cdd:cd05934   276 VIRGLrgwGFFKGYYNMPEATAEAM-RNGW--------FHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAIL 346

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 1947 QCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALP 2022
Cdd:cd05934   347 RHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
A_NRPS_acs4 cd17654
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ...
 1542-2021 3.08e-60

acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341309 [Multi-domain]  Cd Length: 449  Bit Score: 215.41  E-value: 3.08e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFE----DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:cd17654     1 PDRPALIIDqttsDTTVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAAYAPIDPASPEQRS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLTQANQRALltgdvPRILLDTADFSHLSEDnphvpgldaHHLAYVIYTSGSTGKPKGVMNSHRALCNR 1697
Cdd:cd17654    81 LTVMKKCHVSYLLQNKELDNA-----PLSFTPEHRHFNIRTD---------ECLAYVIHTSGTTGTPKIVAVPHKCILPN 146

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1698 LVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQ-LIERTGITTLHFVPSMLQQFVQ 1776
Cdd:cd17654   147 IQHFRSLFNITSEDILFLTSPLTFDPSVVEIFLSLSSGATLLIVPTSVKVLPSKLADiLFKRHRITVLQATPTLFRRFGS 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1777 WADAD---CACDSLRRVICSGEALPAELQQRFFARFNAQLH--NLYGPTEAAIDVTFWACQPDDhrSFVPIGRPIANTQL 1851
Cdd:cd17654   227 QSIKStvlSATSSLRVLALGGEPFPSLVILSSWRGKGNRTRifNIYGITEVSCWALAYKVPEED--SPVQLGSPLLGTVI 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1852 YILDTLGQPvplgVAGELHIGgvGVARGYlnrpdltaerFIPDPfINQPGARLYKTGDLARwLPDGSLEYLGRNDFQVKL 1931
Cdd:cd17654   305 EVRDQNGSE----GTGQVFLG--GLNRVC----------ILDDE-VTVPKGTMRATGDFVT-VKDGELFFLGRKDSQIKR 366

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1932 RGFRIELGEIEARLMQCPGVqEAVVVAREDspgDTRLVAYLCPQPGVTPDPADLRQQLgqhLAEYMVPGAFVTLDAFPLT 2011
Cdd:cd17654   367 RGKRINLDLIQQVIESCLGV-ESCAVTLSD---QQRLIAFIVGESSSSRIHKELQLTL---LSSHAIPDTFVQIDKLPLT 439

                         490
                  ....*....|
gi 641744967 2012 PNGKLDRKAL 2021
Cdd:cd17654   440 SHGKVDKSEL 449
A_NRPS_alphaAR cd17647
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ...
 500-966 4.68e-60

Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.


Pssm-ID: 341302 [Multi-domain]  Cd Length: 520  Bit Score: 217.39  E-value: 4.68e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  500 TYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALltqsa 579
Cdd:cd17647    22 TYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPPARQNIYLGVAKPRGL----- 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  580 qvaqlnstlptVLLDTPAAAACPDTNPVVQglhaahlayviYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIA 659
Cdd:cd17647    97 -----------IVIRAAGVVVGPDSNPTLS-----------FTSGSEGIPKGVLGRHFSLAYYFPWMAKRFNLSENDKFT 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  660 LNASIVFDASVKN-WIQLLSGHTLvLVPDALR-ADAHQLWRYFARHAVDLFDCTPVQLQwLLDAGlGSDPAYQPAQVLIG 737
Cdd:cd17647   155 MLSGIAHDPIQRDmFTPLFLGAQL-LVPTQDDiGTPGRLAEWMAKYGATVTHLTPAMGQ-LLTAQ-ATTPFPKLHHAFFV 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  738 GEAISPAVWSRLQSLSDT-RFINVYGPTECTVDATACVVDRTQPLPTI----------GKPLANTRLYILDAQD--QPVP 804
Cdd:cd17647   232 GDILTKRDCLRLQTLAENvRIVNMYGTTETQRAVSYFEVPSRSSDPTFlknlkdvmpaGRGMLNVQLLVVNRNDrtQICG 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  805 IGVTGELHIGGAGVARGYLHRPDLTAERFI------PD--------------PFSADPAARIYKTGDLARWLPDGNIDYL 864
Cdd:cd17647   312 IGEVGEIYVRAGGLAEGYRGLPELNKEKFVnnwfvePDhwnyldkdnnepwrQFWLGPRDRLYRTGDLGRYLPNGDCECC 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  865 GRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAEL---------------------- 922
Cdd:cd17647   392 GRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRRDKDEEPTLVSYIVPRFDKPDdesfaqedvpkevstdpivkgl 471

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967  923 ---HPAA--LRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAP 966
Cdd:cd17647   472 igyRKLIkdIREFLKKRLASYAIPSLIVVLDKLPLNPNGKVDKPKLQFP 520
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
 1554-2016 5.40e-60

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 214.55  E-value: 5.40e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASpvalltqa 1633
Cdd:cd05903     2 LTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAK-------- 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 nqralltgdvPRILLDTADFSHLSednpHVPGLDAhhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRV 1713
Cdd:cd05903    74 ----------AKVFVVPERFRQFD----PAAMPDA--VALLLFTSGTTGEPKGVMHSHNTLSASIRQYAERLGLGPGDVF 137

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1714 LQKTPFS-FDVSVWEFFWPLLYGARLVMARPdghKDAAYLAQLIERTGITTLHFVPSMLQQFVQWAD-ADCACDSLRRVI 1791
Cdd:cd05903   138 LVASPMAhQTGFVYGFTLPLLLGAPVVLQDI---WDPDKALALMREHGVTFMMGATPFLTDLLNAVEeAGEPLSRLRTFV 214

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1792 CSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHRSFVPiGRPIANTQLYILDTLGQPVPLGVAGELHI 1871
Cdd:cd05903   215 CGGATVPRSLARRAAELLGAKVCSAYGSTECPGAVTSITPAPEDRRLYTD-GRPLPGVEIKVVDDTGATLAPGVEGELLS 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1872 GGVGVARGYLNRPDLTAeRFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGV 1951
Cdd:cd05903   294 RGPSVFLGYLDRPDLTA-DAAPEGW--------FRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGV 364

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 1952 QEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQL-GQHLAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:cd05903   365 IEAAVVALPDERLGERACAVVVTKSGALLTFDELVAYLdRQGVAKQYWPERLVHVDDLPRTPSGKV 430
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
 470-963 9.98e-60

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 215.92  E-value: 9.98e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  470 PREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAA 549
Cdd:PRK07656    2 NEWMTLPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  550 YVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLNST---LPT----VLLDTPAAA-------------ACPDTNPVVQ 609
Cdd:PRK07656   82 VVPLNTRYTADEAAYILARGDAKALFVLGLFLGVDYSAttrLPAlehvVICETEEDDphtekmktftdflAAGDPAERAP 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  610 GLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSR--IALNASIVFDASVkNWIQ-LLSGHTLVLVP 686
Cdd:PRK07656  162 EVDPDDVADILFTSGTTGRPKGAMLTHRQLLSNAADWAEYLGLTEGDRylAANPFFHVFGYKA-GVNApLMRGATILPLP 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  687 dalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLI-GGEAISPAVWSRLQSLSDTRFI-NVYGPT 764
Cdd:PRK07656  241 ---VFDPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAEDLSSLRLAVtGAASMPVALLERFESELGVDIVlTGYGLS 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  765 ECTVDATACVVDRTQPLP--TIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadp 842
Cdd:PRK07656  318 EASGVTTFNRLDDDRKTVagTIGTAIAGVENKIVNELGEEVPVGEVGELLVRGPNVMKGYYDDPEATAAAIDADGW---- 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  843 aarIYkTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIaredspG--DTRL----VAYLCA 916
Cdd:PRK07656  394 ---LH-TGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVI------GvpDERLgevgKAYVVL 463

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*..
gi 641744967  917 RPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK07656  464 KPGAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRAL 510
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
2609-3106 6.29e-59

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 215.36  E-value: 6.29e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2609 HELFEAQVACTPDAIAVVF-----GEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAY 2683
Cdd:COG0365    12 YNCLDRHAEGRGDKVALIWegedgEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVH 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2684 VPLDPTYPVERLRYMLDDAKPVALISQSAhlGIMNG-----------------SLPVILLDDGETRPFDNEPDTPLDARK 2746
Cdd:COG0365    92 SPVFPGFGAEALADRIEDAEAKVLITADG--GLRGGkvidlkekvdealeelpSLEHVIVVGRTGADVPMEGDLDWDELL 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2747 QGLTPR---------HLAYVIYTSGSTGKPKGVMVEHAN-MVNFLCSMRKEPGIAQEDVLLGVTSLSF-----DIsileI 2811
Cdd:COG0365   170 AAASAEfepeptdadDPLFILYTSGTTGKPKGVVHTHGGyLVHAATTAKYVLDLKPGDVFWCTADIGWatghsYI----V 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2812 FLPLLNGARLILATQAQAADAQQLAM-LIERHAVSFMQATPSTWRML-----VELRDFALPPgFKAL-CGGEALPENLAT 2884
Cdd:COG0365   246 YGPLLNGATVVLYEGRPDFPDPGRLWeLIEKYGVTVFFTAPTAIRALmkagdEPLKKYDLSS-LRLLgSAGEPLNPEVWE 324

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2885 ALLQKV-TTLWNLYGPTETT-IWSTLNGLTTPTP-YIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGA--GVVRGYLGR 2959
Cdd:COG0365   325 WWYEAVgVPIVDGWGQTETGgIFISNLPGLPVKPgSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGPwpGMFRGYWND 404

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2960 PDLTAERFiTDPFSGapearMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSP 3039
Cdd:COG0365   405 PERYRETY-FGRFPG-----WYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEI 478

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 3040 GDKRLVAYLLAQPDTVLEPA---DLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL-------PAPDQSAMA 3106
Cdd:COG0365   479 RGQVVKAFVVLKPGVEPSDElakELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLrkiaegrPLGDTSTLE 555
ligase_PEP_1 TIGR03098
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ...
 2607-3099 2.27e-58

acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.


Pssm-ID: 211788 [Multi-domain]  Cd Length: 517  Bit Score: 212.33  E-value: 2.27e-58
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2607 LIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPL 2686
Cdd:TIGR03098    1 LLHHLLEDAAARLPDATALVHHDRTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPI 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2687 DPTYPVERLRYMLDDAKPVALISQSAHLGIMNGSLP-------VILLDDGETRPFDNEPDTPLDARK-QGLTPRH----- 2753
Cdd:TIGR03098   81 NPLLKAEQVAHILADCNVRLLVTSSERLDLLHPALPgchdlrtLIIVGDPAHASEGHPGEEPASWPKlLALGDADpphpv 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2754 ----LAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQA 2829
Cdd:TIGR03098  161 idsdMAAILYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGATVVLHDYLLP 240

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2830 ADAQQlamLIERHAVSFMQATPSTWRMLVELrdfALPPG------FKALCGGeALPENLATALLQKV--TTLWNLYGPTE 2901
Cdd:TIGR03098  241 RDVLK---ALEKHGITGLAAVPPLWAQLAQL---DWPESaapslrYLTNSGG-AMPRATLSRLRSFLpnARLFLMYGLTE 313

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2902 TTIWSTLN-GLTTPTP-YIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERF-ITDPFSGA--- 2975
Cdd:TIGR03098  314 AFRSTYLPpEEVDRRPdSIGKAIPNAEVLVLREDGSECAPGEEGELVHRGALVAMGYWNDPEKTAERFrPLPPFPGElhl 393

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2976 PEARMYkTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTV 3055
Cdd:TIGR03098  394 PELAVW-SGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTLGQAIVLVVTPPGGEE 472

                          490       500       510       520
                   ....*....|....*....|....*....|....*....|....
gi 641744967  3056 LEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPA 3099
Cdd:TIGR03098  473 LDRAALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
 1545-2021 4.95e-58

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 208.86  E-value: 4.95e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1545 TAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDA 1624
Cdd:cd05919     2 TAFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDC 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1625 SPVALLTqanqralltgdvprilldtadfshlsednphvpglDAHHLAYVIYTSGSTGKPKGVMNSHRAL---CNRlvWM 1701
Cdd:cd05919    82 EARLVVT-----------------------------------SADDIAYLLYSSGTTGPPKGVMHAHRDPllfADA--MA 124

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVL--QKTPFSFDV--SVWeffWPLLYGARLVMArpDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQW 1777
Cdd:cd05919   125 REALGLTPGDRVFssAKMFFGYGLgnSLW---FPLAVGASAVLN--PGWPTAERVLATLARFRPTVLYGVPTFYANLLDS 199

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1778 ADADCACD-SLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIdvTFWACQPDDHRsFVPIGRPIANTQLYILDT 1856
Cdd:cd05919   200 CAGSPDALrSLRLCVSAGEALPRGLGERWMEHFGGPILDGIGATEVGH--IFLSNRPGAWR-LGSTGRPVPGYEIRLVDE 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1857 LGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFipdpfinQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRI 1936
Cdd:cd05919   277 EGHTIPPGEEGDLLVRGPSAAVGYWNNPEKSRATF-------NGG--WYRTGDKFCRDADGWYTHAGRADDMLKVGGQWV 347

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1937 ELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPAdLRQQLGQHLAE----YMVPGAFVTLDAFPLTP 2012
Cdd:cd05919   348 SPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQES-LARDIHRHLLErlsaHKVPRRIAFVDELPRTA 426


                  ....*....
gi 641744967 2013 NGKLDRKAL 2021
Cdd:cd05919   427 TGKLQRFKL 435
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
 1533-2021 1.07e-57

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 209.92  E-value: 1.07e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1533 LVEDQAARTPDTTAVLFEDQ-HLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:cd05959     8 LVDLNLNEGRGDKTAFIDDAgSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTL 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 YPAERLAYMLDD-------ASP--VALLTQANQRALLTG---DVPRILLDTADFSHLSEDNPHVPGLD------AHHLAY 1673
Cdd:cd05959    88 LTPDDYAYYLEDsrarvvvVSGelAPVLAAALTKSEHTLvvlIVSGGAGPEAGALLLAELVAAEAEQLkpaathADDPAF 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1674 VIYTSGSTGKPKGVMNSHRALcnrlVWMQNTY-----RLTPDDRVLQ--KTPFSFDVSVWEFFwPLLYGARLVM--ARPD 1744
Cdd:cd05959   168 WLYSSGSTGRPKGVVHLHADI----YWTAELYarnvlGIREDDVCFSaaKLFFAYGLGNSLTF-PLSVGATTVLmpERPT 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1745 ghkdAAYLAQLIERTGITTLHFVP----SMLQQFVQWADADcacDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPT 1820
Cdd:cd05959   243 ----PAAVFKRIRRYRPTVFFGVPtlyaAMLAAPNLPSRDL---SSLRLCVSAGEALPAEVGERWKARFGLDILDGIGST 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1821 EAA-IdvtFWACQPDDHRsFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIpdpfinq 1899
Cdd:cd05959   316 EMLhI---FLSNRPGRVR-YGTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKTRDTFQ------- 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1900 pgARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVT 1979
Cdd:cd05959   385 --GEWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRPGYE 462

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*
gi 641744967 1980 P---DPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05959   463 DseaLEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKL 507
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
 2619-3094 1.62e-57

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 207.08  E-value: 1.62e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:cd17631     8 HPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPEVAYI 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDakpvalisqsahlgimngSLPVILLDDgetrpfdnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMV 2778
Cdd:cd17631    88 LAD------------------SGAKVLFDD-------------------------LALLMYTSGTTGRPKGAMLTHRNLL 124

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2779 NFLCSMRKEPGIAQEDVLLGVTSLsFDISILEIFLP--LLNGARLILATQAQAADAQQlamLIERHAVSFMQATPSTWRM 2856
Cdd:cd17631   125 WNAVNALAALDLGPDDVLLVVAPL-FHIGGLGVFTLptLLRGGTVVILRKFDPETVLD---LIERHRVTSFFLVPTMIQA 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2857 LVELRDFALP--PGFKALC-GGEALPENLATALLQKVTTLWNLYGPTETTiwstlNGLTTPTP--------YIGHPIANT 2925
Cdd:cd17631   201 LLQHPRFATTdlSSLRAVIyGGAPMPERLLRALQARGVKFVQGYGMTETS-----PGVTFLSPedhrrklgSAGRPVFFV 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2926 QIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmyKTGDLGRWLPDGTLEYLGRNDFQV 3005
Cdd:cd17631   276 EVRIVDPDGREVPPGEVGEIVVRGPHVMAGYWNRPEATAAAFRDGWF---------HTGDLGRLDEDGYLYIVDRKKDMI 346

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3006 KVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFP 3085
Cdd:cd17631   347 ISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAELDEDELIAHCRERLARYKIPKSVEFVDALP 426


                  ....*....
gi 641744967 3086 LTPNGKLDR 3094
Cdd:cd17631   427 RNATGKILK 435
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
 1530-2021 1.92e-57

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 210.00  E-value: 1.92e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLd 1609
Cdd:COG1021    27 LGDLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIPVFA- 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1610 pgYPAER---LAYMLDDASPVALLTQA-----NQRAL---LTGDVPR----ILLDTAD----FSHLSED--NPHVPGLDA 1668
Cdd:COG1021   106 --LPAHRraeISHFAEQSEAVAYIIPDrhrgfDYRALareLQAEVPSlrhvLVVGDAGeftsLDALLAApaDLSEPRPDP 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1669 HHLAYVIYTSGSTGKPKGVMNSHralcnrlvwmqNTY-----------RLTPDDRVLQKTP--FSFDVSVWEFFWPLLYG 1735
Cdd:COG1021   184 DDVAFFQLSGGTTGLPKLIPRTH-----------DDYlysvrasaeicGLDADTVYLAALPaaHNFPLSSPGVLGVLYAG 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1736 ARLVMArPDGHKDAAYlaQLIERTGITTLHFVPSMLQ---QFVQWADADCAcdSLRRVICSGEALPAELQQRFFARFNAQ 1812
Cdd:COG1021   253 GTVVLA-PDPSPDTAF--PLIERERVTVTALVPPLALlwlDAAERSRYDLS--SLRVLQVGGAKLSPELARRVRPALGCT 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1813 LHNLYGPTEAAIDVTfwacQPDD--HRSFVPIGRPI-ANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAE 1889
Cdd:COG1021   328 LQQVFGMAEGLVNYT----RLDDpeEVILTTQGRPIsPDDEVRIVDEDGNPVPPGEVGELLTRGPYTIRGYYRAPEHNAR 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1890 RFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLV 1969
Cdd:COG1021   404 AFTPDGF--------YRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSC 475

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|...
gi 641744967 1970 AYLCPQpGVTPDPADLRQQL-GQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:COG1021   476 AFVVPR-GEPLTLAELRRFLrERGLAAFKLPDRLEFVDALPLTAVGKIDKKAL 527
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1561-2021 1.25e-56

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 205.37  E-value: 1.25e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1561 RRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAA----YVPLDPGYPAERLAYMLDDASPVALLTQANQR 1636
Cdd:cd05922     1 LGVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRlglvFVPLNPTLKESVLRYLVADAGGRIVLADAGAA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1637 ALL------TGDvPRILLDTADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPD 1710
Cdd:cd05922    81 DRLrdalpaSPD-PGTVLDADGIRAARASAPAHE-VSHEDLALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGITAD 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1711 DRVLQKTPFSFDVSVWEFFWPLLYGARLVMArPDGHKDAAYLaQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRV 1790
Cdd:cd05922   159 DRALTVLPLSYDYGLSVLNTHLLRGATLVLT-NDGVLDDAFW-EDLREHGATGLAGVPSTYAMLTRLGFDPAKLPSLRYL 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1791 ICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFwaCQPDD-HRSFVPIGRPIANTQLYILDTLGQPVPLGVAGE 1868
Cdd:cd05922   237 TQAGGRLPQETIARLRELLpGAQVYVMYGQTEATRRMTY--LPPERiLEKPGSIGLAIPGGEFEILDDDGTPTPPGEPGE 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1869 LHIGGVGVARGYLNRPdltaeRFIPDPfiNQPGARLYkTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQC 1948
Cdd:cd05922   315 IVHRGPNVMKGYWNDP-----PYRRKE--GRGGGVLH-TGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSI 386

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967 1949 PGVQEAVVVAREDSPGDtRLVAYLCPQPGVtpDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05922   387 GLIIEAAAVGLPDPLGE-KLALFVTAPDKI--DPKDVLRSLAERLPPYKVPATVRVVDELPLTASGKVDYAAL 456
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
 499-963 1.44e-56

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 206.39  E-value: 1.44e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:cd05926    15 LTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLTPK 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 A------------QVAQLNSTLPTVLLDTPAAA----ACPDTNPVVQGLHAAH---LAYVIYTSGSTGRPKGVMVAHRNV 639
Cdd:cd05926    95 GelgpasraasklGLAILELALDVGVLIRAPSAeslsNLLADKKNAKSEGVPLpddLALILHTSGTTGRPKGVPLTHRNL 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  640 INLATGLHTLLALDhPSRIALNASIVFD-----ASVKNwiQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQ 714
Cdd:cd05926   175 AASATNITNTYKLT-PDDRTLVVMPLFHvhglvASLLS--TLAAGGSVVLPP---RFSASTFWPDVRDYNATWYTAVPTI 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  715 LQWLLdaglgSDPAYQPAQVLI-------GGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATacvvdrTQPLP----- 782
Cdd:cd05926   249 HQILL-----NRPEPNPESPPPklrfirsCSASLPPAVLEALEATFGAPVLEAYGMTEAAHQMT------SNPLPpgprk 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  783 --TIGKPlANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGN 860
Cdd:cd05926   318 pgSVGKP-VGVEVRILDEDGEILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGW--------FRTGDLGYLDADGY 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  861 IDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMI 940
Cdd:cd05926   389 LFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASVTEEELRAFCRKHLAAFKV 468

                         490       500
                  ....*....|....*....|...
gi 641744967  941 PSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05926   469 PKKVYFVDELPKTATGKIQRRKV 491
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
 495-958 1.52e-56

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 205.91  E-value: 1.52e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  495 EDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAP--- 571
Cdd:cd05911     7 TGKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPkvi 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  572 -------------VALLTQSAQVAQLNSTLPTVL----LDTPAAAACPDTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMV 634
Cdd:cd05911    87 ftdpdglekvkeaAKELGPKDKIIVLDDKPDGVLsiedLLSPTLGEEDEDLPPPLKDGKDDTAAILYSSGTTGLPKGVCL 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  635 AHRNVINlatglHTLLALDHPSRIALNASIVFDASVKNWI--------QLLSGHTLVLVPdalRADAHQLWRYFARHAVD 706
Cdd:cd05911   167 SHRNLIA-----NLSQVQTFLYGNDGSNDVILGFLPLYHIyglfttlaSLLNGATVIIMP---KFDSELFLDLIEKYKIT 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  707 LFDCTPVQLQWLLDaglgsDPAYQPAQ------VLIGGEAISPAVWSRLQSL-SDTRFINVYGPTECTVdATACVVDRTQ 779
Cdd:cd05911   239 FLYLVPPIAAALAK-----SPLLDKYDlsslrvILSGGAPLSKELQELLAKRfPNATIKQGYGMTETGG-ILTVNPDGDD 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  780 PLPTIGKPLANTRLYILDAQ-DQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPD 858
Cdd:cd05911   313 KPGSVGRLLPNVEAKIVDDDgKDSLGPNEPGEICVRGPQVMKGYYNNPEATKETFDEDGW--------LHTGDIGYFDED 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  859 GNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADY 938
Cdd:cd05911   385 GYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEKLTEKEVKDYVAKKVASY 464

                         490       500
                  ....*....|....*....|....*.
gi 641744967  939 ------MIpsaFVtlDALPLTPNGKL 958
Cdd:cd05911   465 kqlrggVV---FV--DEIPKSASGKI 485
A_NRPS_acs4 cd17654
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ...
 499-963 1.07e-55

acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341309 [Multi-domain]  Cd Length: 449  Bit Score: 202.32  E-value: 1.07e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:cd17654    17 VSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAAYAPIDPASPEQRSLTVMKKCHVSYLLQNK 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 AQ-VAQLNSTLPTVLLDTPaaaacpdtnpvvqglHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDhPSR 657
Cdd:cd17654    97 ELdNAPLSFTPEHRHFNIR---------------TDECLAYVIHTSGTTGTPKIVAVPHKCILPNIQHFRSLFNIT-SED 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  658 IALNASI-VFDASVKNWI-QLLSGHTLVLVPDALRADAHQLWR-YFARHAVDLFDCTPVQLQWLLDAGLGSD--PAYQPA 732
Cdd:cd17654   161 ILFLTSPlTFDPSVVEIFlSLSSGATLLIVPTSVKVLPSKLADiLFKRHRITVLQATPTLFRRFGSQSIKSTvlSATSSL 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  733 QVL-IGGEA--ISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPvpigVTG 809
Cdd:cd17654   241 RVLaLGGEPfpSLVILSSWRGKGNRTRIFNIYGITEVSCWALAYKVPEEDSPVQLGSPLLGTVIEVRDQNGSE----GTG 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  810 ELHIGgaGVARGYlhrpdltaerFIPDPFSAdPAARIYKTGDLARwLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLR 889
Cdd:cd17654   317 QVFLG--GLNRVC----------ILDDEVTV-PKGTMRATGDFVT-VKDGELFFLGRKDSQIKRRGKRINLDLIQQVIES 382

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 641744967  890 CPGVQdAVVIAREDspgDTRLVAYLCARP-DAELHPAALRQQLAAsladYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd17654   383 CLGVE-SCAVTLSD---QQRLIAFIVGESsSSRIHKELQLTLLSS----HAIPDTFVQIDKLPLTSHGKVDKSEL 449
PRK07798 PRK07798
acyl-CoA synthetase; Validated
 479-959 1.53e-55

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 204.35  E-value: 1.53e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  479 FEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:PRK07798    9 FEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYV 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTQS---AQVAQLNSTLPTVLL-----DTPAAAACPDTNPVVQGLHAAHLA-----------YV 619
Cdd:PRK07798   89 EDELRYLLDDSDAVALVYERefaPRVAEVLPRLPKLRTlvvveDGSGNDLLPGAVDYEDALAAGSPErdfgerspddlYL 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  620 IYTSGSTGRPKGVMVAHRNV-------INLATG--LHTLlaLDHPSRIALN-ASIVFDAS--------VKNWIQLLSGHT 681
Cdd:PRK07798  169 LYTGGTTGMPKGVMWRQEDIfrvllggRDFATGepIEDE--EELAKRAAAGpGMRRFPAPplmhgagqWAAFAALFSGQT 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  682 LVLVPDAlRADAHQLWRYFARHAVDLFDCT------PvqlqwLLDAGLGSDPAYQPAQVLI--GGEAISPAVWSRLQSLS 753
Cdd:PRK07798  247 VVLLPDV-RFDADEVWRTIEREKVNVITIVgdamarP-----LLDALEARGPYDLSSLFAIasGGALFSPSVKEALLELL 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  754 DTR-FINVYGPTECTVDATACVVDRTQP--LPTIGkplANTRLYILDAQDQPVP--IGVTGELHIGGAgVARGYLHRPDL 828
Cdd:PRK07798  321 PNVvLTDSIGSSETGFGGSGTVAKGAVHtgGPRFT---IGPRTVVLDEDGNPVEpgSGEIGWIARRGH-IPLGYYKDPEK 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  829 TAERFipdpFSADpAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDT 908
Cdd:PRK07798  397 TAETF----PTID-GVRYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDERWGQ 471

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 641744967  909 RLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLD 959
Cdd:PRK07798  472 EVVAVVQLREGARPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGKAD 522
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
 1550-2021 2.69e-55

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 203.25  E-value: 2.69e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1550 EDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAicverSLDM-----VIGLLAILKAGAAYVPLDPGYPAERLAYMLDDA 1624
Cdd:cd12119    22 EVHRYTYAEVAERARRLANALRRLGVKPGDRVA-----TLAWnthrhLELYYAVPGMGAVLHTINPRLFPEQIAYIINHA 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1625 SPVALLTQANQRALLTGDVPR-------ILLDTADFSHLSEDNP---------------HVPGLDAHHLAYVIYTSGSTG 1682
Cdd:cd12119    97 EDRVVFVDRDFLPLLEAIAPRlptvehvVVMTDDAAMPEPAGVGvlayeellaaespeyDWPDFDENTAAAICYTSGTTG 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1683 KPKGVMNSHRALcnrlvWMQNTYRLTPD-------DRVLQKTPFsFDVSVWEF-FWPLLYGARLVMarPDGHKDAAYLAQ 1754
Cdd:cd12119   177 NPKGVVYSHRSL-----VLHAMAALLTDglglsesDVVLPVVPM-FHVNAWGLpYAAAMVGAKLVL--PGPYLDPASLAE 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1755 LIERTGITTLHFVPSMLQQFVQWADA-DCACDSLRRVICSGEALPAELQQRFFARFNAQLHnLYGPTEAAIDVTFwaCQP 1833
Cdd:cd12119   249 LIEREGVTFAAGVPTVWQGLLDHLEAnGRDLSSLRRVVIGGSAVPRSLIEAFEERGVRVIH-AWGMTETSPLGTV--ARP 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1834 DDHRSFVPI----------GRPIANTQLYILDTLGQPVPL-GVA-GELHIGGVGVARGYLNRPDlTAERFIPDPFInqpg 1901
Cdd:cd12119   326 PSEHSNLSEdeqlalrakqGRPVPGVELRIVDDDGRELPWdGKAvGELQVRGPWVTKSYYKNDE-ESEALTEDGWL---- 400

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1902 arlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPD 1981
Cdd:cd12119   401 ----RTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVT 476

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967 1982 PADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd12119   477 AEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKAL 516
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
 499-958 1.45e-53

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 195.68  E-value: 1.45e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTqs 578
Cdd:cd05903     2 LTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVV-- 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 aqvaqlnstlPTVLLDTPAAAAcPDTnpvvqglhaahLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRI 658
Cdd:cd05903    80 ----------PERFRQFDPAAM-PDA-----------VALLLFTSGTTGEPKGVMHSHNTLSASIRQYAERLGLGPGDVF 137

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  659 aLNASivfdaSVKNWIQLLSGHTLVLVpdaLRADAHQLWRYFARHAVDLFD---CTPVQ-----LQWLLDAGLGSDPAYQ 730
Cdd:cd05903   138 -LVAS-----PMAHQTGFVYGFTLPLL---LGAPVVLQDIWDPDKALALMRehgVTFMMgatpfLTDLLNAVEEAGEPLS 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  731 PAQVLI-GGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRT-QPLPTIGKPLANTRLYILDAQDQPVPIGVT 808
Cdd:cd05903   209 RLRTFVcGGATVPRSLARRAAELLGAKVCSAYGSTECPGAVTSITPAPEdRRLYTDGRPLPGVEIKVVDDTGATLAPGVE 288

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  809 GELHIGGAGVARGYLHRPDLTAeRFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLL 888
Cdd:cd05903   289 GELLSRGPSVFLGYLDRPDLTA-DAAPEGW--------FRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLL 359

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967  889 RCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQL-AASLADYMIPSAFVTLDALPLTPNGKL 958
Cdd:cd05903   360 GHPGVIEAAVVALPDERLGERACAVVVTKSGALLTFDELVAYLdRQGVAKQYWPERLVHVDDLPRTPSGKV 430
starter-C_NRPS cd19533
Starter Condensation domains, found in the first module of nonribosomal peptide synthetases ...
 1068-1498 1.62e-53

Starter Condensation domains, found in the first module of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. While standard C-domains catalyze peptide bond formation between two amino acids, an initial, ('starter') C-domain may instead acylate an amino acid with a fatty acid. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380456 [Multi-domain]  Cd Length: 419  Bit Score: 194.90  E-value: 1.62e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1068 LPLSFSQQRLWFLAQLDPAASQaYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLgFSLS 1147
Cdd:cd19533     2 LPLTSAQRGVWFAEQLDPEGSI-YNLAEYLEITGPVDLAVLERALRQVIAEAETLRLRFTEEEGEPYQWIDPYTP-VPIR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1148 SHDLRKlDEAARTTrVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALE 1227
Cdd:cd19533    80 HIDLSG-DPDPEGA-AQQWMQEDLRKPLPLDNDPLFRHALFTLGDNRHFWYQRVHHIVMDGFSFALFGQRVAEIYTALLK 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1228 GSEANLPPLPvQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAP--ALLEIPTDRPRPSVQRYAGDQVPfhLDAGQLRRL 1305
Cdd:cd19533   158 GRPAPPAPFG-SFLDLVEEEQAYRQSERFERDRAFWTEQFEDLPepVSLARRAPGRSLAFLRRTAELPP--ELTRTLLEA 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1306 HALNRQQGTTLFMTLLAAWsvvLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALR--TEPGRchAVADLLDQVRE 1383
Cdd:cd19533   235 AEAHGASWPSFFIALVAAY---LHRLTGANDVVLGVPVMGRLGAAARQTPGMVANTLPLRltVDPQQ--TFAELVAQVSR 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1384 RALDAYAHQALPFEQVVEILQPARSLsySPIFQVmlSLNNTPAQaltlPDLTLSAVERPQHsTHF-----DLSLSLIET- 1457
Cdd:cd19533   310 ELRSLLRHQRYRYEDLRRDLGLTGEL--HPLFGP--TVNYMPFD----YGLDFGGVVGLTH-NLSsgptnDLSIFVYDRd 380

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|..
gi 641744967 1458 -ENGLNGGLVYATDLFDRETILRvvgYVENILMAMADDVTQP 1498
Cdd:cd19533   381 dESGLRIDFDANPALYSGEDLAR---HQERLLRLLEEAAADP 419
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
 2631-3092 7.55e-53

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 193.75  E-value: 7.55e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQ 2710
Cdd:cd05903     1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2711 sahlgimngslpvillddGETRPFDNEPDtpldarkqgltPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGI 2790
Cdd:cd05903    81 ------------------ERFRQFDPAAM-----------PDAVALLLFTSGTTGEPKGVMHSHNTLSASIRQYAERLGL 131

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2791 AQEDVLLGVTSLSFDI-SILEIFLPLLNGARLILATQAQAADAQQlamLIERHAVSFMQATPSTWRMLVELRDFA---LP 2866
Cdd:cd05903   132 GPGDVFLVASPMAHQTgFVYGFTLPLLLGAPVVLQDIWDPDKALA---LMREHGVTFMMGATPFLTDLLNAVEEAgepLS 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2867 PGFKALCGGEALPENL----ATALLQKVTTLWnlyGPTETtiwSTLNGLTTPTPYI------GHPIANTQIYILDAQGRV 2936
Cdd:cd05903   209 RLRTFVCGGATVPRSLarraAELLGAKVCSAY---GSTEC---PGAVTSITPAPEDrrlytdGRPLPGVEIKVVDDTGAT 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2937 VPLGVAGEIHIAGAGVVRGYLGRPDLTAErfitdpfsgAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGE 3016
Cdd:cd05903   283 LAPGVEGELLSRGPSVFLGYLDRPDLTAD---------AAPEGWFRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLE 353

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 3017 IETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLS-EGVAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:cd05903   354 VEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTFDELVAYLDrQGVAKQYWPERLVHVDDLPRTPSGKV 430
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 1555-2021 1.07e-51

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 190.34  E-value: 1.07e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1555 TYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTqan 1634
Cdd:cd05971     8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT--- 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1635 qralltgdvprillDTADfshlsednphvpgldahHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVL 1714
Cdd:cd05971    85 --------------DGSD-----------------DPALIIYTSGTTGPPKGALHAHRVLLGHLPGVQFPFNLFPRDGDL 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1715 QKTPFS-------FDVsvweFFWPLLYGARLVMARPDGHkDAAYLAQLIERTGITTLHFVPSML----QQFVQWADADCa 1783
Cdd:cd05971   134 YWTPADwawigglLDV----LLPSLYFGVPVLAHRMTKF-DPKAALDLMSRYGVTTAFLPPTALkmmrQQGEQLKHAQV- 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1784 cdSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTfwacqpdDHRSFVPI-----GRPIANTQLYILDTLG 1858
Cdd:cd05971   208 --KLRAIATGGESLGEELLGWAREQFGVEVNEFYGQTECNLVIG-------NCSALFPIkpgsmGKPIPGHRVAIVDDNG 278

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1859 QPVPLGVAGElhiggVGVAR-------GYLNRPDLTAERFIPDPFinqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKL 1931
Cdd:cd05971   279 TPLPPGEVGE-----IAVELpdpvaflGYWNNPSATEKKMAGDWL---------LTGDLGRKDSDGYFWYVGRDDDVITS 344

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1932 RGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDAF 2008
Cdd:cd05971   345 SGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDAlarEIQELVKTRLAAHEYPREIEFVNEL 424

                         490
                  ....*....|...
gi 641744967 2009 PLTPNGKLDRKAL 2021
Cdd:cd05971   425 PRTATGKIRRREL 437
CT_NRPS-like cd19542
Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike ...
 2161-2573 1.26e-51

Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike bacterial NRPS, which typically have specialized terminal thioesterase (TE) domains to cyclize peptide products, many fungal NRPSs employ a terminal condensation-like (CT) domain to produce macrocyclic peptidyl products (e.g. cyclosporine and echinocandin). Domains in this subfamily (which includes both terminal and non-terminal domains) typically have a non-canonical conserved [SN]HxxxDx(14)Y motif at their active site compared to the standard Condensation (C) domain active site motif (HHxxxD). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380464 [Multi-domain]  Cd Length: 401  Bit Score: 189.05  E-value: 1.26e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2161 IYPLAPLQEGILFHHLlqEQGDTYLLRSMVAFthRERLDA--FLSALQQVIDRHDILRTA-VCWQDLSQPVQVVWRQAIL 2237
Cdd:cd19542     1 IYPCTPMQEGMLLSQL--RSPGLYFNHFVFDL--DSSVDVerLRNAWRQLVQRHDILRTVfVESSAEGTFLQVVLKSLDP 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2238 PINHFEptSPEDVLAQlqaHTEPRTRRIDLSQAPLFRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQA 2317
Cdd:cd19542    77 PIEEVE--TDEDSLDA---LTRDLLDDPTLFGQPPHRLTLLETS-SGEVYLVLRISHALYDGVSLPIILRDLAAAYNGQL 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2318 daLPTPLPYRNFIAQTLSVPNSAHEAYFRDKLADVdEPTAPFGLLNVQGSGGDIHearlVLDATLASaIRQQARHLGVSP 2397
Cdd:cd19542   151 --LPPAPPFSDYISYLQSQSQEESLQYWRKYLQGA-SPCAFPSLSPKRPAERSLS----STRRSLAK-LEAFCASLGVTL 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2398 GVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADRIMGMFINTLPLRISLA-DRGAAEVVERTSHDLMTLLEHEQA 2476
Cdd:cd19542   223 ASLFQAAWALVLARYTGSRDVVFGYVVSGRDLPVPGIDDIVGPCINTLPVRVKLDpDWTVLDLLRQLQQQYLRSLPHQHL 302

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2477 PLALAQRCSGVAPPMPLFSTLLNYRHTQA--SSTDNTLSDIRVLTSEERTNYPLTLAVDDRGEGFSLVAQTLEDIDPHRL 2554
Cdd:cd19542   303 SLREIQRALGLWPSGTLFNTLVSYQNFEAspESELSGSSVFELSAAEDPTEYPVAVEVEPSGDSLKVSLAYSTSVLSEEQ 382

                         410
                  ....*....|....*....
gi 641744967 2555 LNYLMTAISSLVDALETEP 2573
Cdd:cd19542   383 AEELLEQFDDILEALLANP 401
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
 488-963 2.76e-51

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 189.04  E-value: 2.76e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  488 EAIAVLFEDQHLTYRELNRRANQLAHHLIALG-VQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd05941     1 DRIAIVDDGDSITYADLVARAARLANRLLALGkDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPvalltqsaqvaqlnstlpTVLLDtpaaaacpdtnpvvqglhaahLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd05941    81 TDSEP------------------SLVLD---------------------PALILYTSGTTGRPKGVVLTHANLAANVRAL 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 ----------HTLLALD----HPSRIALNASivfdasvknwiqLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTP 712
Cdd:cd05941   122 vdawrwteddVLLHVLPlhhvHGLVNALLCP------------LFAGASVEFLP---KFDPKEVAISRLMPSITVFMGVP 186

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  713 ------VQLQWLLDAGLGSDPAYQPAQVLI---GGEAISPAVWSRLQSLSDTRFINVYGPTEcTVDATACVVDRTQPLPT 783
Cdd:cd05941   187 tiytrlLQYYEAHFTDPQFARAAAAERLRLmvsGSAALPVPTLEEWEAITGHTLLERYGMTE-IGMALSNPLDGERRPGT 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  784 IGKPLANTRLYILDAQ-DQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNID 862
Cdd:cd05941   266 VGMPLPGVQARIVDEEtGEPLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDDGW--------FKTGDLGVVDEDGYYW 337

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  863 YLGR-NDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAE-LHPAALRQQLAASLADYMI 940
Cdd:cd05941   338 ILGRsSVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGAAaLSLEELKEWAKQRLAPYKR 417

                         490       500
                  ....*....|....*....|...
gi 641744967  941 PSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05941   418 PRRLILVDELPRNAMGKVNKKEL 440
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
 1520-2021 5.80e-51

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 189.46  E-value: 5.80e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1520 TEADFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAIL 1599
Cdd:cd05920     7 RAAGYWQDEPLGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALL 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1600 KAGAAYVPLDPGYPAERLAYMLDDASPVALLTQANQRalltGDVPRilldtADFSHLSEDNPHVpgldahhlAYVIYTSG 1679
Cdd:cd05920    87 RLGAVPVLALPSHRRSELSAFCAHAEAVAYIVPDRHA----GFDHR-----ALARELAESIPEV--------ALFLLSGG 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1680 STGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTP--FSFDVSVWEFFWPLLYGARLVMArPDGHKDAAYlaQLIE 1757
Cdd:cd05920   150 TTGTPKLIPRTHNDYAYNVRASAEVCGLDQDTVYLAVLPaaHNFPLACPGVLGTLLAGGRVVLA-PDPSPDAAF--PLIE 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1758 RTGITTLHFVPSMLQQFVQ---WADADCAcdSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTfwacQPD 1834
Cdd:cd05920   227 REGVTVTALVPALVSLWLDaaaSRRADLS--SLRLLQVGGARLSPALARRVPPVLGCTLQQVFGMAEGLLNYT----RLD 300

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1835 D--HRSFVPIGRPI-ANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLA 1911
Cdd:cd05920   301 DpdEVIIHTQGRPMsPDDEIRVVDEEGNPVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPDGF--------YRTGDLV 372

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1912 RWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPgVTPDPADLRQQL-G 1990
Cdd:cd05920   373 RRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVLRD-PPPSAAQLRRFLrE 451

                         490       500       510
                  ....*....|....*....|....*....|.
gi 641744967 1991 QHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05920   452 RGLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
 483-963 6.43e-51

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 189.46  E-value: 6.43e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:cd05920    25 AARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLALPSHRRSEL 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLtqsaqVAQLNSTLPTVLLDTPAAAACPDTnpvvqglhaahlAYVIYTSGSTGRPKGVMVAHRNVINL 642
Cdd:cd05920   105 SAFCAHAEAVAYI-----VPDRHAGFDHRALARELAESIPEV------------ALFLLSGGTTGTPKLIPRTHNDYAYN 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  643 ATGLHTLLALDHPSR--IALNASIVFDASVKNWI-QLLSGHTLVLVPDALRADAHQLwryFARHAVDLFDCTPVQLQWLL 719
Cdd:cd05920   168 VRASAEVCGLDQDTVylAVLPAAHNFPLACPGVLgTLLAGGRVVLAPDPSPDAAFPL---IEREGVTVTALVPALVSLWL 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  720 DAGLGSDPAYQPAQVL-IGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATAC-----VVDRTQplptiGKPL-ANTR 792
Cdd:cd05920   245 DAAASRRADLSSLRLLqVGGARLSPALARRVPPVLGCTLQQVFGMAEGLLNYTRLddpdeVIIHTQ-----GRPMsPDDE 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  793 LYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIK 872
Cdd:cd05920   320 IRVVDEEGNPVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPDGF--------YRTGDLVRRTPDGYLVVEGRIKDQIN 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  873 VRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARpDAELHPAALRQQL-AASLADYMIPSAFVTLDALP 951
Cdd:cd05920   392 RGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVLR-DPPPSAAQLRRFLrERGLAAYKLPDRIEFVDSLP 470

                         490
                  ....*....|..
gi 641744967  952 LTPNGKLDRKAL 963
Cdd:cd05920   471 LTAVGKIDKKAL 482
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
 2631-3092 7.40e-51

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 189.35  E-value: 7.40e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQ 2710
Cdd:cd05911    10 ELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFTD 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2711 SAHL----GIMNGSLP---VILLDDGE---------TRPFDNEPDTPLDARKqGLTPRHLAYVIYTSGSTGKPKGVMVEH 2774
Cdd:cd05911    90 PDGLekvkEAAKELGPkdkIIVLDDKPdgvlsiedlLSPTLGEEDEDLPPPL-KDGKDDTAAILYSSGTTGLPKGVCLSH 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2775 ANMVN--FLCSMRKEPGIAQEDVLLGVTSLsFDISILEIFL-PLLNGARLILatqAQAADAQQLAMLIERHAVSFMQATP 2851
Cdd:cd05911   169 RNLIAnlSQVQTFLYGNDGSNDVILGFLPL-YHIYGLFTTLaSLLNGATVII---MPKFDSELFLDLIEKYKITFLYLVP 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2852 STWRMLVE---LRDFALPPGFKALCGGEALPENLAtALLQKVTTLWNL---YGPTETTIWSTLNGLTTPTP-YIGHPIAN 2924
Cdd:cd05911   245 PIAAALAKsplLDKYDLSSLRVILSGGAPLSKELQ-ELLAKRFPNATIkqgYGMTETGGILTVNPDGDDKPgSVGRLLPN 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2925 TQIYILDAQGR-VVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDF 3003
Cdd:cd05911   324 VEAKIVDDDGKdSLGPNEPGEICVRGPQVMKGYYNNPEATKETFDEDGW--------LHTGDIGYFDEDGYLYIVDRKKE 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3004 QVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEY------MIpsa 3077
Cdd:cd05911   396 LIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEKLTEKEVKDYVAKKVASYkqlrggVV--- 472

                         490
                  ....*....|....*
gi 641744967 3078 FVtlDAFPLTPNGKL 3092
Cdd:cd05911   473 FV--DEIPKSASGKI 485
PRK07798 PRK07798
acyl-CoA synthetase; Validated
 1533-2017 8.08e-51

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 190.48  E-value: 8.08e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1533 LVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGY 1612
Cdd:PRK07798    8 LFEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRY 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1613 PAERLAYMLDDASPVALLTQ-------ANQRALLTGdvPRILLDTADFSHLSEDNPHVPGLDAhhLA------------- 1672
Cdd:PRK07798   88 VEDELRYLLDDSDAVALVYErefaprvAEVLPRLPK--LRTLVVVEDGSGNDLLPGAVDYEDA--LAagsperdfgersp 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1673 ---YVIYTSGSTGKPKGVMNSH----RALCNRLVWMQNTYRLTPDDRVLQKT-----------PFSFDVSVWEFFWPLLY 1734
Cdd:PRK07798  164 ddlYLLYTGGTTGMPKGVMWRQedifRVLLGGRDFATGEPIEDEEELAKRAAagpgmrrfpapPLMHGAGQWAAFAALFS 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1735 GARLVMArPDGHKDAAYLAQLIERTGITTLHFV------PsMLQQFVQWADADCAcdSLRRVICSGEALPAELQQRFFAR 1808
Cdd:PRK07798  244 GQTVVLL-PDVRFDADEVWRTIEREKVNVITIVgdamarP-LLDALEARGPYDLS--SLFAIASGGALFSPSVKEALLEL 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1809 F-NAQLHNLYGPTEA---AIDVTFWACQPDDHRSFvPIGRPIAntqlyILDTLGQPVPLG--VAGELHIGGVgVARGYLN 1882
Cdd:PRK07798  320 LpNVVLTDSIGSSETgfgGSGTVAKGAVHTGGPRF-TIGPRTV-----VLDEDGNPVEPGsgEIGWIARRGH-IPLGYYK 392

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1883 RPDLTAERFipdPFINqpGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDS 1962
Cdd:PRK07798  393 DPEKTAETF---PTID--GVRYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDE 467

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1963 PGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:PRK07798  468 RWGQEVVAVVQLREGARPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGKAD 522
PRK08316 PRK08316
acyl-CoA synthetase; Validated
 1538-2016 9.44e-51

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 189.76  E-value: 9.44e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:PRK08316   21 ARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEEL 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLTQANQRALL-------------------TGDVPRILLDTADFSHLSEDNPHVPGLDAHHLAYVIYTS 1678
Cdd:PRK08316  101 AYILDHSGARAFLVDPALAPTAeaalallpvdtlilslvlgGREAPGGWLDFADWAEAGSVAEPDVELADDDLAQILYTS 180

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1679 GSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPF----SFDVsvweFFWPLLY-GAR-LVMARPdghkDAAYL 1752
Cdd:PRK08316  181 GTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIPLHALPLyhcaQLDV----FLGPYLYvGATnVILDAP----DPELI 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1753 AQLIERTGITTLhFVP-----SMLQ--QFvqwADADCAcdSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAI 1824
Cdd:PRK08316  253 LRTIEAERITSF-FAPptvwiSLLRhpDF---DTRDLS--SLRKGYYGASIMPVEVLKELRERLpGLRFYNCYGQTEIAP 326

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1825 DVTfwACQPDDH-RSFVPIGRPIANTQLYILDTLGQPVPLGVAGELhiggvgVAR------GYLNRPDLTAERFIPDPFi 1897
Cdd:PRK08316  327 LAT--VLGPEEHlRRPGSAGRPVLNVETRVVDDDGNDVAPGEVGEI------VHRspqlmlGYWDDPEKTAEAFRGGWF- 397

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1898 nqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPG 1977
Cdd:PRK08316  398 --------HSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAG 469

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967 1978 VTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:PRK08316  470 ATVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKI 508
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
 1554-2021 1.23e-50

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 186.77  E-value: 1.23e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTqa 1633
Cdd:cd05972     1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT-- 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 nqralltgdvprilldtadfshlsednphvpglDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRV 1713
Cdd:cd05972    79 ---------------------------------DAEDPALIYFTSGTTGLPKGVLHTHSYPLGHIPTAAYWLGLRPDDIH 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1714 LQKTPFSFDVSVW-EFFWPLLYGARLVMARPDGHKDAAYLaQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVIC 1792
Cdd:cd05972   126 WNIADPGWAKGAWsSFFGPWLLGATVFVYEGPRFDAERIL-ELLERYGVTSFCGPPTAYRMLIKQDLSSYKFSHLRLVVS 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1793 SGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHRSfvpIGRPIANTQLYILDTLGQPVPLGVAGEL--H 1870
Cdd:cd05972   205 AGEPLNPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMPVKPGS---MGRPTPGYDVAIIDDDGRELPPGEEGDIaiK 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1871 IGGVGVARGYLNRPDLTAERFIPDpfinqpgarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPG 1950
Cdd:cd05972   282 LPPPGLFLGYVGDPEKTEASIRGD---------YYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPA 352

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 1951 VQEAVVVAREDsPGDTRLV-AYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPG--AFVtlDAFPLTPNGKLDRKAL 2021
Cdd:cd05972   353 VAEAAVVGSPD-PVRGEVVkAFVVLTSGYEPSEElaeELQGHVKKVLAPYKYPReiEFV--EELPKTISGKIRRVEL 426
Condensation pfam00668
Condensation domain; This domain is found in many multi-domain enzymes which synthesize ...
 2158-2592 1.48e-50

Condensation domain; This domain is found in many multi-domain enzymes which synthesize peptide antibiotics. This domain catalyzes a condensation reaction to form peptide bonds in non- ribosomal peptide biosynthesis. It is usually found to the carboxy side of a phosphopantetheine binding domain (pfam00550). It has been shown that mutations in the HHXXXDG motif abolish activity suggesting this is part of the active site.


Pssm-ID: 395541 [Multi-domain]  Cd Length: 454  Bit Score: 187.54  E-value: 1.48e-50
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2158 IQDIYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQA-- 2235
Cdd:pfam00668    1 VQDEYPLSPAQKRMWFLEKLEPHSSAYNMPAVLKLTGELDPERLEKALQELINRHDALRTVFIRQENGEPVQVILEERpf 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2236 ---ILPINHFEPTSPEDVLAQLQahTEPRTRRIDLSQAPLFRADIaHDPLQNEWLLALSFHHLISDHMTLALIVGEIRLL 2312
Cdd:pfam00668   81 eleIIDISDLSESEEEEAIEAFI--QRDLQSPFDLEKGPLFRAGL-FRIAENRHHLLLSMHHIIVDGVSLGILLRDLADL 157

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2313 LQHQADALPTPLP----YRNFIAQTLSVPNSA----HEAYFRDKLADVDEPTApfgLLNV----QGSGGDIHEARLVLDA 2380
Cdd:pfam00668  158 YQQLLKGEPLPLPpktpYKDYAEWLQQYLQSEdyqkDAAYWLEQLEGELPVLQ---LPKDyarpADRSFKGDRLSFTLDE 234

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2381 TLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRI-SLADRGAAEV 2459
Cdd:pfam00668  235 DTEELLRKLAKAHGTTLNDVLLAAYGLLLSRYTGQDDIVVGTPGSGR--PSPDIERMVGMFVNTLPLRIdPKGGKTFSEL 312

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2460 VERTSHDLMTLLEHEQAPLALAQRCSGV---APPMPLFSTLL---NYRHTQASSTDNTLS--DIRVLTS-EERTNYPLTL 2530
Cdd:pfam00668  313 IKRVQEDLLSAEPHQGYPFGDLVNDLRLprdLSRHPLFDPMFsfqNYLGQDSQEEEFQLSelDLSVSSViEEEAKYDLSL 392

                          410       420       430       440       450       460
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967  2531 AVDDRGEGFSL--VAQT----LEDIDphRLLNYLMTAISSLVDALEtEPQRSILnlpVLPDSERQQML 2592
Cdd:pfam00668  393 TASERGGGLTIkiDYNTslfdEETIE--RFAEHFKELLEQAIAHPS-QPLSELD---LLSDAEKQKLL 454
CT_NRPS-like cd19542
Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike ...
 28-436 2.05e-50

Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike bacterial NRPS, which typically have specialized terminal thioesterase (TE) domains to cyclize peptide products, many fungal NRPSs employ a terminal condensation-like (CT) domain to produce macrocyclic peptidyl products (e.g. cyclosporine and echinocandin). Domains in this subfamily (which includes both terminal and non-terminal domains) typically have a non-canonical conserved [SN]HxxxDx(14)Y motif at their active site compared to the standard Condensation (C) domain active site motif (HHxxxD). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380464 [Multi-domain]  Cd Length: 401  Bit Score: 185.59  E-value: 2.05e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   28 IYPLAPLQEGILFhyQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHQP-VQVVWRQAPLTV 106
Cdd:cd19542     1 IYPCTPMQEGMLL--SQLRSPGLYFNHFVFDLDSSVDVERLRNAWRQLVQRHDILRTVFVESSAEGTfLQVVLKSLDPPI 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  107 NTLTTTSsdtvpAQLRAATDPSNHRLNLSNAPLLSATTAHDPVcGEWLLSLSIHHLISDHITQALIIDEIRLLLEDRPea 186
Cdd:cd19542    79 EEVETDE-----DSLDALTRDLLDDPTLFGQPPHRLTLLETSS-GEVYLVLRISHALYDGVSLPIILRDLAAAYNGQL-- 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  187 LPKPLPYRNFIAQILSVPLSEHEQYFRNRLADIdTPTAPFDLVDVQGngediTEARLSLDSSLADALRRQARHLGISSSV 266
Cdd:cd19542   151 LPPAPPFSDYISYLQSQSQEESLQYWRKYLQGA-SPCAFPSLSPKRP-----AERSLSSTRRSLAKLEAFCASLGVTLAS 224

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  267 LFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGADRVMGMFINTLPLRVSL-RERSVHDVVQATSHELMMLLAHEQAPL 345
Cdd:cd19542   225 LFQAAWALVLARYTGSRDVVFGYVVSGRDLPVPGIDDIVGPCINTLPVRVKLdPDWTVLDLLRQLQQQYLRSLPHQHLSL 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  346 ALAQQCSQVPPPLPLFSTLFNYRHSQKDASSQFWEG--MRQLSGRERTNYPITLSVDDLGDG--FNLTAKT-VMGVD-PE 419
Cdd:cd19542   305 REIQRALGLWPSGTLFNTLVSYQNFEASPESELSGSsvFELSAAEDPTEYPVAVEVEPSGDSlkVSLAYSTsVLSEEqAE 384

                         410
                  ....*....|....*..
gi 641744967  420 RIVHYMLTAIENLVTSL 436
Cdd:cd19542   385 ELLEQFDDILEALLANP 401
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
 491-963 2.12e-50

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 186.51  E-value: 2.12e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  491 AVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAA 570
Cdd:cd05919     3 AFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCE 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  571 PVALLTqsaqvaqlnstlptvlldtpaaaacpdtnpvvqglHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHT-L 649
Cdd:cd05919    83 ARLVVT-----------------------------------SADDIAYLLYSSGTTGPPKGVMHAHRDPLLFADAMAReA 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  650 LALDHPSRIALNASIVFDASVKN--WIQLLSGHTLVLVPDALRADAhqLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDP 727
Cdd:cd05919   128 LGLTPGDRVFSSAKMFFGYGLGNslWFPLAVGASAVLNPGWPTAER--VLATLARFRPTVLYGVPTFYANLLDSCAGSPD 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  728 AYQPAQVLI-GGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPlPTIGKPLANTRLYILDAQDQPVPIG 806
Cdd:cd05919   206 ALRSLRLCVsAGEALPRGLGERWMEHFGGPILDGIGATEVGHIFLSNRPGAWRL-GSTGRPVPGYEIRLVDEEGHTIPPG 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  807 VTGELHIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESR 886
Cdd:cd05919   285 EEGDLLVRGPSAAVGYWNNPEKSRATF---------NGGWYRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESL 355

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  887 LLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDA---ELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05919   356 IIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAapqESLARDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKL 435
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
 2621-3097 6.28e-50

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 185.19  E-value: 6.28e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2621 DAIAVVFGEASLSYDELNRRANRLAHHLI-SFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd05941     1 DRIAIVDDGDSITYADLVARAARLANRLLaLGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISqsahlgimngslpvillddgetrpfdnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVN 2779
Cdd:cd05941    81 TDSEPSLVLD--------------------------------------------PALILYTSGTTGRPKGVVLTHANLAA 116

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRKEPGIAQEDVLLGVTSLsFDIS--ILEIFLPLLNGARLILATQAQAADAQQLAMliERHAVSFMqATPSTWRML 2857
Cdd:cd05941   117 NVRALVDAWRWTEDDVLLHVLPL-HHVHglVNALLCPLFAGASVEFLPKFDPKEVAISRL--MPSITVFM-GVPTIYTRL 192

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2858 VELRDFALPPGFKAL-----------CGGEALPENLaTALLQKVT--TLWNLYGPTETtiwstlnGLTTPTPY------- 2917
Cdd:cd05941   193 LQYYEAHFTDPQFARaaaaerlrlmvSGSAALPVPT-LEEWEAITghTLLERYGMTEI-------GMALSNPLdgerrpg 264

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 -IGHPIANTQIYILDAQ-GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTL 2995
Cdd:cd05941   265 tVGMPLPGVQARIVDEEtGEPLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDDGW--------FKTGDLGVVDEDGYY 336

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2996 EYLGR-NDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPD-TVLEPADLRQRLSEGVAEYM 3073
Cdd:cd05941   337 WILGRsSVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGaAALSLEELKEWAKQRLAPYK 416

                         490       500
                  ....*....|....*....|....
gi 641744967 3074 IPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05941   417 RPRRLILVDELPRNAMGKVNKKEL 440
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
1534-2021 8.64e-50

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 186.22  E-value: 8.64e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1534 VEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLI-DLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGY 1612
Cdd:PRK06839    8 IEKRAYLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLIyELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRL 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1613 PAERLAYMLDDASPVALLTQ---ANQRALLTGDV----PRILLDTADFSHLSEDNPHVPGLDAHHLayVIYTSGSTGKPK 1685
Cdd:PRK06839   88 TENELIFQLKDSGTTVLFVEktfQNMALSMQKVSyvqrVISITSLKEIEDRKIDNFVEKNESASFI--ICYTSGTTGKPK 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1686 G-VMNSHRALCNRLvwmQNTYR--LTPDDRVLQKTPFSFDVSVWEFFWP-LLYGARLVMARpdgHKDAAYLAQLIERTGI 1761
Cdd:PRK06839  166 GaVLTQENMFWNAL---NNTFAidLTMHDRSIVLLPLFHIGGIGLFAFPtLFAGGVIIVPR---KFEPTKALSMIEKHKV 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1762 TTLHFVPSMLQQFVQWAD-ADCACDSLRRVICSGEALPAELQQRFFARfNAQLHNLYGPTEAAIDVtFWACQPDDHRSFV 1840
Cdd:PRK06839  240 TVVMGVPTIHQALINCSKfETTNLQSVRWFYNGGAPCPEELMREFIDR-GFLFGQGFGMTETSPTV-FMLSEEDARRKVG 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1841 PIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERfIPDPFINqpgarlykTGDLARWLPDGSLE 1920
Cdd:PRK06839  318 SIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRPDATEET-IQDGWLC--------TGDLARVDEDGFVY 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1921 YLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPG 2000
Cdd:PRK06839  389 IVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSVLIEKDVIEHCRLFLAKYKIPK 468

                         490       500
                  ....*....|....*....|.
gi 641744967 2001 AFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK06839  469 EIVFLKELPKNATGKIQKAQL 489
DCL_NRPS-like cd19536
DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal ...
 1068-1494 9.05e-50

DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal fungal CT domains and Dual Epimerization/Condensation (E/C) domains; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type [D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L))], which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380459 [Multi-domain]  Cd Length: 419  Bit Score: 184.19  E-value: 9.05e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1068 LPLSFSQQRLWFLAQLDPAASqAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLGFSLS 1147
Cdd:cd19536     2 YPLSSLQEGMLFHSLLNPGGS-VYLHNYTYTVGRRLNLDLLLEALQVLIDRHDILRTSFIEDGLGQPVQVVHRQAQVPVT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1148 SHDLRKLDEAarTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLL-LTQHHIISDGWSIGILARELAALYQAAL 1226
Cdd:cd19536    81 ELDLTPLEEQ--LDPLRAYKEETKIRRFDLGRAPLVRAALVRKDERERFLLvISDHHSILDGWSLYLLVKEILAVYNQLL 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1227 EGSEANLPPlPVQYADYAVWQR-QWLQGETLNdlrdYWRDQLQGA-----PALLEIPTDRP-RPSVQRyagdqVPFHLDA 1299
Cdd:cd19536   159 EYKPLSLPP-AQPYRDFVAHERaSIQQAASER----YWREYLAGAtlatlPALSEAVGGGPeQDSELL-----VSVPLPV 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1300 gqlrRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQ--ELEGMVGFFVNTLALRTEPGRcHAVADL 1377
Cdd:cd19536   229 ----RSRSLAKRSGIPLSTLLLAAWALVLSRHSGSDDVVFGTVVHGRSEEttGAERLLGLFLNTLPLRVTLSE-ETVEDL 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1378 LDQVRERALDAYAHQALPfeqVVEILQPARSLsysPIFQVMLSLNNTP-AQALTLPDLTLSAVERPQHS---THFDLSLS 1453
Cdd:cd19536   304 LKRAQEQELESLSHEQVP---LADIQRCSEGE---PLFDSIVNFRHFDlDFGLPEWGSDEGMRRGLLFSefkSNYDVNLS 377

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|.
gi 641744967 1454 LIETENGLNGGLVYATDLFDRETILRVVGYVENILMAMADD 1494
Cdd:cd19536   378 VLPKQDRLELKLAYNSQVLDEEQAQRLAAYYKSAIAELATA 418
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 506-963 1.40e-49

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 184.57  E-value: 1.40e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  506 RRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAA----YVPMDPAYPAERLAYILDDAAPVALLTQSAQV 581
Cdd:cd05922     1 LGVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRlglvFVPLNPTLKESVLRYLVADAGGRIVLADAGAA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  582 AQLNSTL-----PTVLLDTPAAAACPDTNPVVQGLHAaHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPS 656
Cdd:cd05922    81 DRLRDALpaspdPGTVLDADGIRAARASAPAHEVSHE-DLALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGITADD 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  657 RIALNASIVFDA--SVKNwIQLLSGHTLVLVPDALRADAhqLWRYFARHAVDLFDCTPVQLQWLLDAGLgsDPAYQP--- 731
Cdd:cd05922   160 RALTVLPLSYDYglSVLN-THLLRGATLVLTNDGVLDDA--FWEDLREHGATGLAGVPSTYAMLTRLGF--DPAKLPslr 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  732 --AQVligGEAISPAVWSRLQSL-SDTRFINVYGPTECTVDATACVVDRTQPLPT-IGKPLANTRLYILDAQDQPVPIGV 807
Cdd:cd05922   235 ylTQA---GGRLPQETIARLRELlPGAQVYVMYGQTEATRRMTYLPPERILEKPGsIGLAIPGGEFEILDDDGTPTPPGE 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  808 TGELHIGGAGVARGYLHRPdltaeRFIPDPfsADPAARIYkTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRL 887
Cdd:cd05922   312 PGEIVHRGPNVMKGYWNDP-----PYRRKE--GRGGGVLH-TGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAA 383

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967  888 LRCPGVQDAVVIAREDSPGDtRLVayLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05922   384 RSIGLIIEAAAVGLPDPLGE-KLA--LFVTAPDKIDPKDVLRSLAERLPPYKVPATVRVVDELPLTASGKVDYAAL 456
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
 479-963 2.13e-49

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 186.12  E-value: 2.13e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  479 FEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVpMdpAYP 558
Cdd:COG1021    31 LRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIPV-F--ALP 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AER---LAYILDDAAPVALLTQS-----------AQVAQLNSTLPTV--------------LLDTPAAAACPDTNPvvqg 610
Cdd:COG1021   108 AHRraeISHFAEQSEAVAYIIPDrhrgfdyralaRELQAEVPSLRHVlvvgdageftsldaLLAAPADLSEPRPDP---- 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  611 lhaAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSR------IALNASI----VFDAsvknwiqLLSGH 680
Cdd:COG1021   184 ---DDVAFFQLSGGTTGLPKLIPRTHDDYLYSVRASAEICGLDADTVylaalpAAHNFPLsspgVLGV-------LYAGG 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  681 TLVLVPDAlraDAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVL-IGGEAISPAVWSRLQSLSDTRFIN 759
Cdd:COG1021   254 TVVLAPDP---SPDTAFPLIERERVTVTALVPPLALLWLDAAERSRYDLSSLRVLqVGGAKLSPELARRVRPALGCTLQQ 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  760 VYGPTE----CT-VDATACVVDRTQplptiGKPLAN---TRlyILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAE 831
Cdd:COG1021   331 VFGMAEglvnYTrLDDPEEVILTTQ-----GRPISPddeVR--IVDEDGNPVPPGEVGELLTRGPYTIRGYYRAPEHNAR 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  832 RFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLV 911
Cdd:COG1021   404 AFTPDGF--------YRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSC 475

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|...
gi 641744967  912 AYLCARpDAELHPAALRQQLAAS-LADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:COG1021   476 AFVVPR-GEPLTLAELRRFLRERgLAAFKLPDRLEFVDALPLTAVGKIDKKAL 527
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
 1554-2021 4.69e-49

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 182.29  E-value: 4.69e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA 1633
Cdd:cd05935     2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGS 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 NQralltgdvprilldtadfshlsEDnphvpgldahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRV 1713
Cdd:cd05935    82 EL----------------------DD-----------LALIPYTSGTTGLPKGCMHTHFSAAANALQSAVWTGLTPSDVI 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1714 LQKTPFsFDVS--VWEFFWPLLYGARLV-MARPDghKDAAylAQLIERTGITTLHFVPSMLQQFV---QWADADCAcdSL 1787
Cdd:cd05935   129 LACLPL-FHVTgfVGSLNTAVYVGGTYVlMARWD--RETA--LELIEKYKVTFWTNIPTMLVDLLatpEFKTRDLS--SL 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1788 RRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFwacQPDDHRSFVPIGRPIANTQLYILD-TLGQPVPLGVA 1866
Cdd:cd05935   202 KVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGLTETMSQTHT---NPPLRPKLQCLGIP*FGVDARVIDiETGRELPPNEV 278

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1867 GELHIGGVGVARGYLNRPDLTAERFIPDPfinqpGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLM 1946
Cdd:cd05935   279 GEIVVRGPQIFKGYWNRPEETEESFIEIK-----GRRFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLY 353

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 1947 QCPGVQEAVVVAREDSPGDTRLVAYLCPQPGV--TPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05935   354 KHPAI*EVCVISVPDERVGEEVKAFIVLRPEYrgKVTEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
 2620-3097 6.45e-49

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 184.34  E-value: 6.45e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:PRK07656   19 GDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYTADEAAYIL 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQSAHLGI---MNGSLP----VILLDDGETRPFDNEPDT--------PLDARKQGLTPRHLAYVIYTSGST 2764
Cdd:PRK07656   99 ARGDAKALFVLGLFLGVdysATTRLPalehVVICETEEDDPHTEKMKTftdflaagDPAERAPEVDPDDVADILFTSGTT 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2765 GKPKGVMVEHANMVNflcsmrkepgiAQEDV--LLGVTSLSFDISILEIF----------LPLLNGARLILATQAQAADA 2832
Cdd:PRK07656  179 GRPKGAMLTHRQLLS-----------NAADWaeYLGLTEGDRYLAANPFFhvfgykagvnAPLMRGATILPLPVFDPDEV 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2833 QQlamLIERHAVSFMQATPSTWRML---VELRDFALPPGFKALCGGEALPENLATALLQK--VTTLWNLYGPTETTIWST 2907
Cdd:PRK07656  248 FR---LIETERITVLPGPPTMYNSLlqhPDRSAEDLSSLRLAVTGAASMPVALLERFESElgVDIVLTGYGLSEASGVTT 324

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2908 LNGL-----TTPTPyIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearMYk 2982
Cdd:PRK07656  325 FNRLdddrkTVAGT-IGTAIAGVENKIVNELGEEVPVGEVGELLVRGPNVMKGYYDDPEATAAAIDADGW-------LH- 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2983 TGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIaredspG--DKRL----VAYLLAQPDTVL 3056
Cdd:PRK07656  396 TGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVI------GvpDERLgevgKAYVVLKPGAEL 469

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 641744967 3057 EPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK07656  470 TEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRAL 510
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
 1503-2021 1.19e-48

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 184.49  E-value: 1.19e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1503 PILpDSERRQVMldfnaTEADFPHDALIHQLVEDQAARTPDTTAVL------FEDQHLTYDALNRRANQLAHHLIDLGVK 1576
Cdd:PRK13295    5 AVL-LPPRRAAS-----IAAGHWHDRTINDDLDACVASCPDKTAVTavrlgtGAPRRFTYRELAALVDRVAVGLARLGVG 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1577 PDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQANQR-----ALLTG------DVPR 1645
Cdd:PRK13295   79 RGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIFRERELSFMLKHAESKVLVVPKTFRgfdhaAMARRlrpelpALRH 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1646 ILL---DTAD-F-SHLS----EDNPHVPGL------DAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPD 1710
Cdd:PRK13295  159 VVVvggDGADsFeALLItpawEQEPDAPAIlarlrpGPDDVTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGAD 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1711 DRVLQKTPFSFDVSvweffwpLLYGARL-VMARPDG----HKDAAYLAQLIERTGIT-TLHFVP--SMLQQFVQWADADC 1782
Cdd:PRK13295  239 DVILMASPMAHQTG-------FMYGLMMpVMLGATAvlqdIWDPARAAELIRTEGVTfTMASTPflTDLTRAVKESGRPV 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1783 AcdSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIdVTFWACQPDDHRSFVPIGRPIANTQLYILDTLGQPVP 1862
Cdd:PRK13295  312 S--SLRTFLCAGAPIPGALVERARAALGAKIVSAWGMTENGA-VTLTKLDDPDERASTTDGCPLPGVEVRVVDADGAPLP 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1863 LGVAGELHIGGVGVARGYLNRPDLTAERFipDPFinqpgarlYKTGDLARWLPDGSLEYLGRNDfQVKLRGFR-IELGEI 1941
Cdd:PRK13295  389 AGQIGRLQVRGCSNFGGYLKRPQLNGTDA--DGW--------FDTGDLARIDADGYIRISGRSK-DVIIRGGEnIPVVEI 457

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1942 EARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLG-QHLAEYMVPGAFVTLDAFPLTPNGKLDRKA 2020
Cdd:PRK13295  458 EALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFEEMVEFLKaQKVAKQYIPERLVVRDALPRTPSGKIQKFR 537


                  .
gi 641744967 2021 L 2021
Cdd:PRK13295  538 L 538
PRK06188 PRK06188
acyl-CoA synthetase; Validated
 1539-2024 2.45e-48

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 182.88  E-value: 2.45e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1539 ARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLA 1618
Cdd:PRK06188   23 KRYPDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAAQLAGLRRTALHPLGSLDDHA 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1619 YMLDDASPVALLTQANQ---------------RALLT-GDVPrillDTADFSHLS---EDNPHVPGLDAHHLAYVIYTSG 1679
Cdd:PRK06188  103 YVLEDAGISTLIVDPAPfveralallarvpslKHVLTlGPVP----DGVDLLAAAakfGPAPLVAAALPPDIAGLAYTGG 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1680 STGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVweFFWP-LLYGARLVMARpdGHKDAAYLAqLIER 1758
Cdd:PRK06188  179 TTGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHAGGA--FFLPtLLRGGTVIVLA--KFDPAEVLR-AIEE 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1759 TGITTLHFVPSMLQQFVQWADADCA-CDSLRRVICSGEAL-PAELQQRFfARFNAQLHNLYGPTEAAIDVTFWACQ---P 1833
Cdd:PRK06188  254 QRITATFLVPTMIYALLDHPDLRTRdLSSLETVYYGASPMsPVRLAEAI-ERFGPIFAQYYGQTEAPMVITYLRKRdhdP 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1834 DDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFipdpfinqPGARLYkTGDLARW 1913
Cdd:PRK06188  333 DDPKRLTSCGRPTPGLRVALLDEDGREVAQGEVGEICVRGPLVMDGYWNRPEETAEAF--------RDGWLH-TGDVARE 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1914 LPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHL 1993
Cdd:PRK06188  404 DEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAAELQAHVKERK 483

                         490       500       510
                  ....*....|....*....|....*....|.
gi 641744967 1994 AEYMVPGAFVTLDAFPLTPNGKLDRKALPAP 2024
Cdd:PRK06188  484 GSVHAPKQVDFVDSLPLTALGKPDKKALRAR 514
PRK07787 PRK07787
acyl-CoA synthetase; Validated
 1538-2024 4.45e-48

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 180.57  E-value: 4.45e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHhlidlGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:PRK07787   10 AAAADIADAVRIGGRVLSRSDLAGAATAVAE-----RVAGARRVAVLATPTLATVLAVVGALIAGVPVVPVPPDSGVAER 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLTQANQRAlltGDVPRILLDTADFSHLSEDNPhvpglDAHHLAYVIYTSGSTGKPKGVMNSHRALCNR 1697
Cdd:PRK07787   85 RHILADSGAQAWLGPAPDDP---AGLPHVPVRLHARSWHRYPEP-----DPDAPALIVYTSGTTGPPKGVVLSRRAIAAD 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1698 LVWMQNTYRLTPDDRVLQKTPFsFDVS--VWEFFWPLLYGARLV-MARPdghKDAAYLAQLIERTgitTLHF-VPSMLQQ 1773
Cdd:PRK07787  157 LDALAEAWQWTADDVLVHGLPL-FHVHglVLGVLGPLRIGNRFVhTGRP---TPEAYAQALSEGG---TLYFgVPTVWSR 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1774 FVqwADADCAcDSLR--RVICSGEA-LPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWAcqpDDHRSFVPIGRPIANTQ 1850
Cdd:PRK07787  230 IA--ADPEAA-RALRgaRLLVSGSAaLPVPVFDRLAALTGHRPVERYGMTETLITLSTRA---DGERRPGWVGLPLAGVE 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1851 LYILDTLGQPVPLGVA--GELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGRN--D 1926
Cdd:PRK07787  304 TRLVDEDGGPVPHDGEtvGELQVRGPTLFDGYLNRPDATAAAFTADGW--------FRTGDVAVVDPDGMHRIVGREstD 375

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1927 FqVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDpaDLRQQLGQHLAEYMVPGAFVTLD 2006
Cdd:PRK07787  376 L-IKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVAAD--ELIDFVAQQLSVHKRPREVRFVD 452

                         490
                  ....*....|....*...
gi 641744967 2007 AFPLTPNGKLDRKALPAP 2024
Cdd:PRK07787  453 ALPRNAMGKVLKKQLLSE 470
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
 1523-2016 5.40e-48

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 182.47  E-value: 5.40e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1523 DFPHDALIHQLvEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLID-LGVKPDDRIAICVERSLDMVIGLLAILKA 1601
Cdd:PRK08314    6 TLPETSLFHNL-EVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQQeCGVRKGDRVLLYMQNSPQFVIAYYAILRA 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1602 GAAYVPLDPGYPAERLAYMLDDA-SPVALLTQ--ANQRALLTGD----------------------VPRILLDTADFSHL 1656
Cdd:PRK08314   85 NAVVVPVNPMNREEELAHYVTDSgARVAIVGSelAPKVAPAVGNlrlrhvivaqysdylpaepeiaVPAWLRAEPPLQAL 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1657 SEDN---------------PHVPGLDahHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFsF 1721
Cdd:PRK08314  165 APGGvvawkealaaglappPHTAGPD--DLAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLAVLPL-F 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1722 DVS--VWEFFWPLLYGARLV-MARPDghKDAAylAQLIERTGITTLHFVPSMLQQF---VQWADADCAcdSLRRVICSGE 1795
Cdd:PRK08314  242 HVTgmVHSMNAPIYAGATVVlMPRWD--REAA--ARLIERYRVTHWTNIPTMVVDFlasPGLAERDLS--SLRYIGGGGA 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1796 ALPAELQQRFFARFNAQLHNLYGPTEAAidvTFWACQPDDHRSFVPIGRPIANTQLYILD-TLGQPVPLGVAGELHIGGV 1874
Cdd:PRK08314  316 AMPEAVAERLKELTGLDYVEGYGLTETM---AQTHSNPPDRPKLQCLGIPTFGVDARVIDpETLEELPPGEVGEIVVHGP 392

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1875 GVARGYLNRPDLTAERFIPdpfINqpGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEA 1954
Cdd:PRK08314  393 QVFKGYWNRPEATAEAFIE---ID--GKRFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEA 467

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967 1955 VVVAREDSPGDTRLVAYLCPQPG--VTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:PRK08314  468 CVIATPDPRRGETVKAVVVLRPEarGKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKI 531
PRK07788 PRK07788
acyl-CoA synthetase; Validated
 1533-2025 8.18e-48

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 182.05  E-value: 8.18e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1533 LVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGY 1612
Cdd:PRK07788   54 LVAHAARRAPDRAALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGF 133

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1613 PAERLAYMLDDASPVALLTQANQRALLTG---DVPRILLDTADFSHLSEDNPHVPGLD--------------AHHLAYVI 1675
Cdd:PRK07788  134 SGPQLAEVAAREGVKALVYDDEFTDLLSAlppDLGRLRAWGGNPDDDEPSGSTDETLDdliagsstaplpkpPKPGGIVI 213

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1676 YTSGSTGKPKGVMNSHRALcnrlvwmqntyrLTPDDRVLQKTPF--------------SFDVSVWEFFWPLlyGARLVMA 1741
Cdd:PRK07788  214 LTSGTTGTPKGAPRPEPSP------------LAPLAGLLSRVPFragettllpapmfhATGWAHLTLAMAL--GSTVVLR 279

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1742 RpdgHKDAAYLAQLIERTGITTLHFVPSMLQQFV---QWADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYG 1818
Cdd:PRK07788  280 R---RFDPEATLEDIAKHKATALVVVPVMLSRILdlgPEVLAKYDTSSLKIIFVSGSALSPELATRALEAFGPVLYNLYG 356

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1819 PTEAAIDVTfwaCQPDDHRsFVP--IGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDltaerfipDPF 1896
Cdd:PRK07788  357 STEVAFATI---ATPEDLA-EAPgtVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGYTDGRD--------KQI 424

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1897 INQpgarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQP 1976
Cdd:PRK07788  425 IDG----LLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAP 500

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967 1977 GVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAPD 2025
Cdd:PRK07788  501 GAALDEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREMD 549
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 2633-3098 1.15e-47

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 177.87  E-value: 1.15e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2633 SYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQsa 2712
Cdd:cd05934     5 TYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVVD-- 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2713 hlgimngslpvillddgetrpfdnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQ 2792
Cdd:cd05934    83 -----------------------------------------PASILYTSGTTGPPKGVVITHANLTFAGYYSARRFGLGE 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2793 EDVLLGVTSLsFDISILEI-FLP-LLNGARLILATQAQAADAQQLamlIERHAVSFMQATPSTWRMLvelrdFALPPG-- 2868
Cdd:cd05934   122 DDVYLTVLPL-FHINAQAVsVLAaLSVGATLVLLPRFSASRFWSD---VRRYGATVTNYLGAMLSYL-----LAQPPSpd 192

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2869 -----FKALCGGEALPENLATALLQKVTTLWNLYGPTETT--IWSTLNGlTTPTPYIGHPIANTQIYILDAQGRVVPLGV 2941
Cdd:cd05934   193 drahrLRAAYGAPNPPELHEEFEERFGVRLLEGYGMTETIvgVIGPRDE-PRRPGSIGRPAPGYEVRIVDDDGQELPAGE 271

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2942 AGEIHIAGA---GVVRGYLGRPDLTAERFitdpfsgapEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIE 3018
Cdd:cd05934   272 PGELVIRGLrgwGFFKGYYNMPEATAEAM---------RNGWFHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVE 342

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3019 TRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALP 3098
Cdd:cd05934   343 RAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
menE TIGR01923
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ...
 500-963 1.21e-47

O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]


Pssm-ID: 162605 [Multi-domain]  Cd Length: 436  Bit Score: 178.41  E-value: 1.21e-47
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   500 TYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTqsA 579
Cdd:TIGR01923    1 TWQDLDCEAAHLAKALKAQGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLLLT--D 78

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   580 QVAQLNSTLPTVLLDTPAAAACPDTnpVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSR-- 657
Cdd:TIGR01923   79 SLLEEKDFQADSLDRIEAAGRYETS--LSASFNMDQIATLMFTSGTTGKPKAVPHTFRNHYASAVGSKENLGFTEDDNwl 156

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   658 IAL------NASIVFDAsvknwiqLLSGHTLVlVPDALRADAHQLwryfARHAVDLFDCTPVQLQWLLDAGLgsdPAYQP 731
Cdd:TIGR01923  157 LSLplyhisGLSILFRW-------LIEGATLR-IVDKFNQLLEMI----ANERVTHISLVPTQLNRLLDEGG---HNENL 221

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   732 AQVLIGGEAISPAVWSRLQSLSDTRFiNVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYIldaqDQPVPIGVtGEL 811
Cdd:TIGR01923  222 RKILLGGSAIPAPLIEEAQQYGLPIY-LSYGMTETCSQVTTATPEMLHARPDVGRPLAGREIKI----KVDNKEGH-GEI 295

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   812 HIGGAGVARGYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCP 891
Cdd:TIGR01923  296 MVKGANLMKGYLYQGELTPAFEQQGWF---------NTGDIGELDGEGFLYVLGRRDDLIISGGENIYPEEIETVLYQHP 366

                          410       420       430       440       450       460       470
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967   892 GVQDAVVIAREDSPGDTRLVAYLCARpdAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:TIGR01923  367 GIQEAVVVPKPDAEWGQVPVAYIVSE--SDISQAKLIAYLTEKLAKYKVPIAFEKLDELPYNASGKILRNQL 436
PRK06178 PRK06178
acyl-CoA synthetase; Validated
 1538-2021 3.09e-47

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 180.62  E-value: 3.09e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:PRK06178   43 ARERPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHEL 122

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLTQaNQRALLTGDV--------------------------------PRILL-DTADFSHLSEDNP--- 1661
Cdd:PRK06178  123 SYELNDAGAEVLLAL-DQLAPVVEQVraetslrhvivtsladvlpaeptlplpdslraPRLAAaGAIDLLPALRACTapv 201

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1662 --HVPGLDAhhLAYVIYTSGSTGKPKGVMNSHRalcnRLVWMQNTY-----RLTPDDRVLQKTPFsfdvsvwefFW---- 1730
Cdd:PRK06178  202 plPPPALDA--LAALNYTGGTTGMPKGCEHTQR----DMVYTAAAAyavavVGGEDSVFLSFLPE---------FWiage 266

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1731 ------PLLYGARLV-MARpdghKDAAYLAQLIERTGIT-TLHFVPSM--LQQFVQWADADCAcdSLRRVICSG--EALP 1798
Cdd:PRK06178  267 nfgllfPLFSGATLVlLAR----WDAVAFMAAVERYRVTrTVMLVDNAveLMDHPRFAEYDLS--SLRQVRVVSfvKKLN 340

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1799 AELQQRFFARFNAQLHNL-YGPTEAAIDVTFWA-CQPDDHR-SFVPI--GRPIANTQLYILD-TLGQPVPLGVAGELHIG 1872
Cdd:PRK06178  341 PDYRQRWRALTGSVLAEAaWGMTETHTCDTFTAgFQDDDFDlLSQPVfvGLPVPGTEFKICDfETGELLPLGAEGEIVVR 420

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1873 GVGVARGYLNRPDLTAERFIpDPFinqpgarlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQ 1952
Cdd:PRK06178  421 TPSLLKGYWNKPEATAEALR-DGW--------LHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAVL 491

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 1953 EAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVtLDAFPLTPNGKLDRKAL 2021
Cdd:PRK06178  492 GSAVVGRPDPDKGQVPVAFVQLKPGADLTAAALQAWCRENMAVYKVPEIRI-VDALPMTATGKVRKQDL 559
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
 1545-2021 4.42e-47

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 176.90  E-value: 4.42e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1545 TAVLFEDQHLTYDALNRRANQLAHHLID-LGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDD 1623
Cdd:cd05958     2 TCLRSPEREWTYRDLLALANRIANVLVGeLGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDK 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1624 ASPValltqanqRALLTGDVprilldTAdfshlSEDnphvpgldahhLAYVIYTSGSTGKPKGVMNSHR---ALCNRlvW 1700
Cdd:cd05958    82 ARIT--------VALCAHAL------TA-----SDD-----------ICILAFTSGTTGAPKATMHFHRdplASADR--Y 129

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1701 MQNTYRLTPDDRVLQKTP--FSFDVSVWEFFwPLLYGARLVM---ARPDGhkdaayLAQLIERTGITTLHFVPSMLQQFV 1775
Cdd:cd05958   130 AVNVLRLREDDRFVGSPPlaFTFGLGGVLLF-PFGVGASGVLleeATPDL------LLSAIARYKPTVLFTAPTAYRAML 202

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1776 QWADA---DCACdsLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAidVTFWACQPDDHRsfvP--IGRPIANTQ 1850
Cdd:cd05958   203 AHPDAagpDLSS--LRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEMF--HIFISARPGDAR---PgaTGKPVPGYE 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1851 LYILDTLGQPVPLGVAGELHIGGvgvARGYLNRPDLTAERFIPDPFInqpgarlyKTGDLARWLPDGSLEYLGRNDFQVK 1930
Cdd:cd05958   276 AKVVDDEGNPVPDGTIGRLAVRG---PTGCRYLADKRQRTYVQGGWN--------ITGDTYSRDPDGYFRHQGRSDDMIV 344

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1931 LRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDA 2007
Cdd:cd05958   345 SGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVlarELQDHAKAHIAPYKYPRAIEFVTE 424

                         490
                  ....*....|....
gi 641744967 2008 FPLTPNGKLDRKAL 2021
Cdd:cd05958   425 LPRTATGKLQRFAL 438
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
 490-963 4.44e-47

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 178.72  E-value: 4.44e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  490 IAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDA 569
Cdd:cd05959    21 TAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYLEDS 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  570 APVALLTQSAQVAQLNS-------TLPTVLLDTPA-------------AAACPDTNPVvqGLHAAHLAYVIYTSGSTGRP 629
Cdd:cd05959   101 RARVVVVSGELAPVLAAaltksehTLVVLIVSGGAgpeagalllaelvAAEAEQLKPA--ATHADDPAFWLYSSGSTGRP 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  630 KGVMVAHRNVINLA-TGLHTLLALdHPSRIALNASIVFDA-SVKN--WIQLLSGHTLVLVPDalRADAHQLWRYFARHAV 705
Cdd:cd05959   179 KGVVHLHADIYWTAeLYARNVLGI-REDDVCFSAAKLFFAyGLGNslTFPLSVGATTVLMPE--RPTPAAVFKRIRRYRP 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  706 DLFDCTPVqlqwLLDAGLGSDPAYQPAQVLI-----GGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQP 780
Cdd:cd05959   256 TVFFGVPT----LYAAMLAAPNLPSRDLSSLrlcvsAGEALPAEVGERWKARFGLDILDGIGSTEMLHIFLSNRPGRVRY 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  781 lPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIpdpfsadpaARIYKTGDLARWLPDGN 860
Cdd:cd05959   332 -GTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKTRDTFQ---------GEWTRTGDKYVRDDDGF 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  861 IDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARP---DAELHPAALRQQLAASLAD 937
Cdd:cd05959   402 YTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRPgyeDSEALEEELKEFVKDRLAP 481

                         490       500
                  ....*....|....*....|....*.
gi 641744967  938 YMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05959   482 YKYPRWIVFVDELPKTATGKIQRFKL 507
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
1537-2021 4.96e-47

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 177.85  E-value: 4.96e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1537 QAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAER 1616
Cdd:PRK03640   11 RAFLTPDRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREE 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1617 LAYMLDDASPVALLTQANQRALLTGDVPrilldtADFSHLSE-DNPHVPGLDAHHL---AYVIYTSGSTGKPKGVMNSHr 1692
Cdd:PRK03640   91 LLWQLDDAEVKCLITDDDFEAKLIPGIS------VKFAELMNgPKEEAEIQEEFDLdevATIMYTSGTTGKPKGVIQTY- 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1693 alcnrlvwmQNTYR----------LTPDDRVLQKTPFsFDVSVWE-FFWPLLYGARLVMARpdgHKDAAYLAQLIERTGI 1761
Cdd:PRK03640  164 ---------GNHWWsavgsalnlgLTEDDCWLAAVPI-FHISGLSiLMRSVIYGMRVVLVE---KFDAEKINKLLQTGGV 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1762 TTLHFVPSMLQQFVQWADADCACDSLRRVIC-SGEALPAELQQrffarfnAQLHNL-----YGPTEAAIDVTfwACQPDD 1835
Cdd:PRK03640  231 TIISVVSTMLQRLLERLGEGTYPSSFRCMLLgGGPAPKPLLEQ-------CKEKGIpvyqsYGMTETASQIV--TLSPED 301

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1836 HRSFV-PIGRPIANTQLYILDTlGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlyKTGDLARWL 1914
Cdd:PRK03640  302 ALTKLgSAGKPLFPCELKIEKD-GVVVPPFEEGEIVVKGPNVTKGYLNREDATRETFQDGWF---------KTGDIGYLD 371

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1915 PDGSLEYLGRndfqvklRGFRIELG-------EIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCpqPGVTPDPADLRQ 1987
Cdd:PRK03640  372 EEGFLYVLDR-------RSDLIISGgeniypaEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFVV--KSGEVTEEELRH 442

                         490       500       510
                  ....*....|....*....|....*....|....
gi 641744967 1988 QLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK03640  443 FCEEKLAKYKVPKRFYFVEELPRNASGKLLRHEL 476
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 2639-3097 5.78e-47

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 176.86  E-value: 5.78e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2639 RRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGA----YVPLDPTYPVERLRYMLDDAKP-VALISQSAh 2713
Cdd:cd05922     1 LGVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRlglvFVPLNPTLKESVLRYLVADAGGrIVLADAGA- 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2714 lgimngslpVILLDDGETRPFDnePDTPLDAR----KQGLTPRH------LAYVIYTSGSTGKPKGVMVEHANMVNFLCS 2783
Cdd:cd05922    80 ---------ADRLRDALPASPD--PGTVLDADgiraARASAPAHevshedLALLLYTSGSTGSPKLVRLSHQNLLANARS 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2784 MRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLamLIERHAVSFMQATPSTWRMLVEL-RD 2862
Cdd:cd05922   149 IAEYLGITADDRALTVLPLSYDYGLSVLNTHLLRGATLVLTNDGVLDDAFWE--DLREHGATGLAGVPSTYAMLTRLgFD 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2863 FALPPGFKALC-GGEALPENLATALLQKV--TTLWNLYGPTETTIWSTL----NGLTTPTPyIGHPIANTQIYILDAQGR 2935
Cdd:cd05922   227 PAKLPSLRYLTqAGGRLPQETIARLRELLpgAQVYVMYGQTEATRRMTYlppeRILEKPGS-IGLAIPGGEFEILDDDGT 305

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2936 VVPLGVAGEIHIAGAGVVRGYLGRPdltaeRFITDPfsGAPEARMYkTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELG 3015
Cdd:cd05922   306 PTPPGEPGEIVHRGPNVMKGYWNDP-----PYRRKE--GRGGGVLH-TGDLARRDEDGFLFIVGRRDRMIKLFGNRISPT 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3016 EIETRLARCHGVHDAVVIAREDSPGDkRLVAYLLAQPDtvLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRK 3095
Cdd:cd05922   378 EIEAAARSIGLIIEAAAVGLPDPLGE-KLALFVTAPDK--IDPKDVLRSLAERLPPYKVPATVRVVDELPLTASGKVDYA 454


                  ..
gi 641744967 3096 AL 3097
Cdd:cd05922   455 AL 456
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
477-921 9.61e-47

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 179.91  E-value: 9.61e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  477 QRFEAQAEQTPEAIAVLFED----QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVP 552
Cdd:COG1022    15 DLLRRRAARFPDRVALREKEdgiwQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVP 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  553 MDPAYPAERLAYILDDAAPVALLTQS-AQVAQLNS---TLPT----VLLDTPAAAACPDTNPV----------------- 607
Cdd:COG1022    95 IYPTSSAEEVAYILNDSGAKVLFVEDqEQLDKLLEvrdELPSlrhiVVLDPRGLRDDPRLLSLdellalgrevadpaele 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  608 --VQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSR-------------------IALNASIVF 666
Cdd:COG1022   175 arRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRtlsflplahvfertvsyyaLAAGATVAF 254

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  667 DASVKNWIQLLSG---HTLVLVP-------DALRADAHQ-------LWRYFARHAVDLFDctpvQLQWLLDAGLGSDPAY 729
Cdd:COG1022   255 AESPDTLAEDLREvkpTFMLAVPrvwekvyAGIQAKAEEagglkrkLFRWALAVGRRYAR----ARLAGKSPSLLLRLKH 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  730 QPAQVLI-----------------GGEAISPAVwsrlqslsdTRF-----INV---YGPTECTvdATACVVDRTQPLP-T 783
Cdd:COG1022   331 ALADKLVfsklrealggrlrfavsGGAALGPEL---------ARFfralgIPVlegYGLTETS--PVITVNRPGDNRIgT 399

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  784 IGKPLANTRlyildaqdqpVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDY 863
Cdd:COG1022   400 VGPPLPGVE----------VKIAEDGEILVRGPNVMKGYYKNPEATAEAFDADGW--------LHTGDIGELDEDGFLRI 461

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967  864 LGRNDFQIKVR-GFRIEAGEIESRLLRCPGVQDAVVIaredspGDTR--LVAYLCarPDAE 921
Cdd:COG1022   462 TGRKKDLIVTSgGKNVAPQPIENALKASPLIEQAVVV------GDGRpfLAALIV--PDFE 514
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
 495-963 1.81e-46

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 177.05  E-value: 1.81e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  495 EDQHLTYRELNRRANQLAHHLIALGVQPDDRVA-LCVE--RSLEMMVGLLGIlkaGAAYVPMDPAYPAERLAYILDDAAP 571
Cdd:cd12119    22 EVHRYTYAEVAERARRLANALRRLGVKPGDRVAtLAWNthRHLELYYAVPGM---GAVLHTINPRLFPEQIAYIINHAED 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  572 VALLTQS---AQVAQLNSTLPTV---LLDTPAAAACPDTNPVV----QGLHAA-----------HLAYVI-YTSGSTGRP 629
Cdd:cd12119    99 RVVFVDRdflPLLEAIAPRLPTVehvVVMTDDAAMPEPAGVGVlayeELLAAEspeydwpdfdeNTAAAIcYTSGTTGNP 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  630 KGVMVAHRNVInlatgLHTLlALDHPSRIALNAS-----IVFDASVKNW----IQLLSGHTLVLvPDAlRADAHQLWRYF 700
Cdd:cd12119   179 KGVVYSHRSLV-----LHAM-AALLTDGLGLSESdvvlpVVPMFHVNAWglpyAAAMVGAKLVL-PGP-YLDPASLAELI 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  701 ARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPA-QVLIGGEAISPAVWSRLQSLsDTRFINVYGPTE-CTVDATACVVDRT 778
Cdd:cd12119   251 EREGVTFAAGVPTVWQGLLDHLEANGRDLSSLrRVVIGGSAVPRSLIEAFEER-GVRVIHAWGMTEtSPLGTVARPPSEH 329

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  779 QPLP---------TIGKPLANTRLYILDAQDQPVPI-GVT-GELHIGGAGVARGYLHRPDlTAERFIPDPFsadpaariY 847
Cdd:cd12119   330 SNLSedeqlalraKQGRPVPGVELRIVDDDGRELPWdGKAvGELQVRGPWVTKSYYKNDE-ESEALTEDGW--------L 400

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  848 KTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAAL 927
Cdd:cd12119   401 RTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTAEEL 480

                         490       500       510
                  ....*....|....*....|....*....|....*.
gi 641744967  928 RQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd12119   481 LEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKAL 516
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
 1554-2021 4.12e-46

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 174.32  E-value: 4.12e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTqa 1633
Cdd:cd05907     6 ITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFV-- 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 nqralltgdvprillDTADfshlsednphvpgldahHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRV 1713
Cdd:cd05907    84 ---------------EDPD-----------------DLATIIYTSGTTGRPKGVMLSHRNILSNALALAERLPATEGDRH 131

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1714 LQKTPFS--FDVSVWEFFwPLLYGARLVMARPDghkdaAYLAQLIERTGITTLHFVPSMLQQFV------------QWAD 1779
Cdd:cd05907   132 LSFLPLAhvFERRAGLYV-PLLAGARIYFASSA-----ETLLDDLSEVRPTVFLAVPRVWEKVYaaikvkavpglkRKLF 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1780 ADCACDSLRRVICSGEALPAELqQRFFARFNAQLHNLYGPTEAAIDVTFWacQPDDHRsfvpigrpiantqlyiLDTLGQ 1859
Cdd:cd05907   206 DLAVGGRLRFAASGGAPLPAEL-LHFFRALGIPVYEGYGLTETSAVVTLN--PPGDNR----------------IGTVGK 266

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1860 PVP-----LGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGR-NDFQVKLRG 1933
Cdd:cd05907   267 PLPgvevrIADDGEILVRGPNVMLGYYKNPEATAEALDADGW--------LHTGDLGEIDEDGFLHITGRkKDLIITSGG 338

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1934 FRIELGEIEARLMQCPGVQEAVVVaredspGDTR--LVAYLCPQPGVTPDPA-----------------DLRQQLGQHLA 1994
Cdd:cd05907   339 KNISPEPIENALKASPLISQAVVI------GDGRpfLVALIVPDPEALEAWAeehgiaytdvaelaanpAVRAEIEAAVE 412

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 641744967 1995 E--------------YMVPGAFvTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05907   413 AanarlsryeqikkfLLLPEPF-TIENGELTPTLKLKRPVI 452
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
 1530-2021 8.55e-46

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 174.62  E-value: 8.55e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQ--HLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVP 1607
Cdd:cd05923     3 VFEMLRRAASRAPDACAIADPARglRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPAL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1608 LDPGYPAERLAYML-----------DDASPVALLTQANQRALLTGDVPRILLDTAdFSHLSEDNPHVPGLDahhlAYVIY 1676
Cdd:cd05923    83 INPRLKAAELAELIergemtaaviaVDAQVMDAIFQSGVRVLALSDLVGLGEPES-AGPLIEDPPREPEQP----AFVFY 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1677 TSGSTGKPKGVMNSHRALCNRLVWM--QNTYRLTPDDRVLQKTPFSFDVSVWEFF-WPLLYGARLVMARPDghkDAAYLA 1753
Cdd:cd05923   158 TSGTTGLPKGAVIPQRAAESRVLFMstQAGLRHGRHNVVLGLMPLYHVIGFFAVLvAALALDGTYVVVEEF---DPADAL 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1754 QLIERTGITTLHFVPSMLQQFVQWAD-ADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQ 1832
Cdd:cd05923   235 KLIEQERVTSLFATPTHLDALAAAAEfAGLKLSSLRHVTFAGATMPDAVLERVNQHLPGEKVNIYGTTEAMNSLYMRDAR 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1833 PDDhrsfvpIGRPIANTQLYILDTLGQPV---PLGVAGELHIGGVGVA--RGYLNRPDLTAERFIpdpfinqpgARLYKT 1907
Cdd:cd05923   315 TGT------EMRPGFFSEVRIVRIGGSPDealANGEEGELIVAAAADAafTGYLNQPEATAKKLQ---------DGWYRT 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1908 GDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGvtPDPADLRQ 1987
Cdd:cd05923   380 GDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREG--TLSADELD 457

                         490       500       510
                  ....*....|....*....|....*....|....*.
gi 641744967 1988 Q--LGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05923   458 QfcRASELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
 499-937 1.06e-45

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 173.17  E-value: 1.06e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQs 578
Cdd:cd05907     6 ITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVE- 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 aqvaqlnstlptvlldtpaaaacpdtnpvvqglHAAHLAYVIYTSGSTGRPKGVMVAHRNVI-NLATGLHTLLALDHPSR 657
Cdd:cd05907    85 ---------------------------------DPDDLATIIYTSGTTGRPKGVMLSHRNILsNALALAERLPATEGDRH 131

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  658 IA-LNASIVFDASVKNWIQLLSGHTLVLVPDA--LRAD-----------AHQLWR--YFARHAVDlfdcTPVQLQWLLDA 721
Cdd:cd05907   132 LSfLPLAHVFERRAGLYVPLLAGARIYFASSAetLLDDlsevrptvflaVPRVWEkvYAAIKVKA----VPGLKRKLFDL 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  722 GLGSDPAYqpaqVLIGGEAISPAVWSRLQSLSDTrFINVYGPTECTvdATACVVDRTQPLP-TIGKPLANTRlyildaqd 800
Cdd:cd05907   208 AVGGRLRF----AASGGAPLPAELLHFFRALGIP-VYEGYGLTETS--AVVTLNPPGDNRIgTVGKPLPGVE-------- 272

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  801 qpVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGR-NDFQIKVRGFRIE 879
Cdd:cd05907   273 --VRIADDGEILVRGPNVMLGYYKNPEATAEALDADGW--------LHTGDLGEIDEDGFLHITGRkKDLIITSGGKNIS 342

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  880 AGEIESRLLRCPGVQDAVVIaredspGDTR--LVAYLCARPDAELHPAALRQQLAASLAD 937
Cdd:cd05907   343 PEPIENALKASPLISQAVVI------GDGRpfLVALIVPDPEALEAWAEEHGIAYTDVAE 396
C_NRPS-like cd19537
Condensation family domain with an atypical active site motif; Condensation (C) domains of ...
 1067-1495 1.61e-45

Condensation family domain with an atypical active site motif; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily typically have a non-canonical conserved SHXXXDX(14)Y motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380460 [Multi-domain]  Cd Length: 395  Bit Score: 170.83  E-value: 1.61e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1067 PLPLSFSQQRLWFLAQLDpAASQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQqidpdtlgfSL 1146
Cdd:cd19537     1 DTALSPIEREWWHKYQLS-TGTSSFNVSFACRLSGDVDRDRLASAWNTVLARHRILRSRYVPRDGGLRR---------SY 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1147 SSHdlrkldeAARTTRVAEL-AEQEARARFDLTQGPLIRGqLLQLDdnthVLLLTQHHIISDGWSIGILARELAALYQAA 1225
Cdd:cd19537    71 SSS-------PPRVQRVDTLdVWKEINRPFDLEREDPIRV-FISPD----TLLVVMSHIICDLTTLQLLLREVSAAYNGK 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1226 LegseanLPPLPVQYADYAVWQRQWLQGEtlndlRDYWRDQLQGAPalleiPTDRPRP-SVQRYAGDQVPFHLDAGQLRR 1304
Cdd:cd19537   139 L------LPPVRREYLDSTAWSRPASPED-----LDFWSEYLSGLP-----LLNLPRRtSSKSYRGTSRVFQLPGSLYRS 202

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1305 LHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPRQELEGMVGFFVNTLALR--TEPGRCHAVADLLDQVR 1382
Cdd:cd19537   203 LLQFSTSSGITLHQLALAAVALALQDLSDRTDIVLGAPYLNRTSEEDMETVGLFLEPLPIRirFPSSSDASAADFLRAVR 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1383 ERALDAYAHqALPFEQVVEILQ-PARSLSYsPIFQVMLS--LNNTPAQALTLPDLTlsaverPQHsTH-----FDLSLSL 1454
Cdd:cd19537   283 RSSQAALAH-AIPWHQLLEHLGlPPDSPNH-PLFDVMVTfhDDRGVSLALPIPGVE------PLY-TWaegakFPLMFEF 353

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*
gi 641744967 1455 IETEnglNGGLV----YATDLFDRETILRVVGYVENILMAMADDV 1495
Cdd:cd19537   354 TALS---DDSLLlrleYDTDCFSEEEIDRIESLILAALELLVEGK 395
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
 2620-3097 2.75e-45

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 172.88  E-value: 2.75e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd05926     3 APALVVPGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALI---------SQSAH--------LGIMNGSLpvILLDDGETRPFDNEPDTPLDARKQGLtPRHLAYVIYTSG 2762
Cdd:cd05926    83 ADLGSKLVLtpkgelgpaSRAASklglaileLALDVGVL--IRAPSAESLSNLLADKKNAKSEGVPL-PDDLALILHTSG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2763 STGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLsFDISILEIFL--PLLNGARLILATQAqaadaqqlamlie 2840
Cdd:cd05926   160 TTGRPKGVPLTHRNLAASATNITNTYKLTPDDRTLVVMPL-FHVHGLVASLlsTLAAGGSVVLPPRF------------- 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2841 rHAVSF---MQATPSTWRMLVE-----LRDFALPPGFKAL-------CGGEALPENLATALLQKV-TTLWNLYGPTETTI 2904
Cdd:cd05926   226 -SASTFwpdVRDYNATWYTAVPtihqiLLNRPEPNPESPPpklrfirSCSASLPPAVLEALEATFgAPVLEAYGMTEAAH 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2905 WSTLNglttPTPYIGHPI------ANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapea 2978
Cdd:cd05926   305 QMTSN----PLPPGPRKPgsvgkpVGVEVRILDEDGEILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGW------ 374

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2979 rmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEP 3058
Cdd:cd05926   375 --FRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASVTE 452

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967 3059 ADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05926   453 EELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKV 491
PRK06164 PRK06164
acyl-CoA synthetase; Validated
 1533-2011 3.03e-45

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 174.16  E-value: 3.03e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1533 LVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGY 1612
Cdd:PRK06164   15 LLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRY 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1613 PAERLAYMLDDASPVALLTQANQRALltgDVPRILLDTADFSHLS----------------------------EDNPHVP 1664
Cdd:PRK06164   95 RSHEVAHILGRGRARWLVVWPGFKGI---DFAAILAAVPPDALPPlraiavvddaadatpapapgarvqlfalPDPAPPA 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1665 GL-----DAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLV 1739
Cdd:PRK06164  172 AAgeraaDPDAGALLFTTSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCGVFGFSTLLGALAGGAPLV 251

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1740 MarpDGHKDAAYLAQLIERTGITtlHFVPS--MLQQFVQWADADCACDSLRRV-----ICSGEALPAELQQRffarfNAQ 1812
Cdd:PRK06164  252 C---EPVFDAARTARALRRHRVT--HTFGNdeMLRRILDTAGERADFPSARLFgfasfAPALGELAALARAR-----GVP 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1813 LHNLYGPTEAAIDVTFWACQPDDHRSFVPIGRPI-ANTQLYILDTL-GQPVPLGVAGELHIGGVGVARGYLNRPDLTAER 1890
Cdd:PRK06164  322 LTGLYGSSEVQALVALQPATDPVSVRIEGGGRPAsPEARVRARDPQdGALLPDGESGEIEIRAPSLMRGYLDNPDATARA 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1891 FIPDPFinqpgarlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREdSPGDTRLVA 1970
Cdd:PRK06164  402 LTDDGY--------FRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGAT-RDGKTVPVA 472

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 641744967 1971 YLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLT 2011
Cdd:PRK06164  473 FVIPTDGASPDEAGLMAACREALAGFKVPARVQVVEAFPVT 513
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
 1524-2021 4.00e-45

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 172.80  E-value: 4.00e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1524 FPHDALIHQLVEDQAARTPDTTAvlFED----QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAIL 1599
Cdd:cd05904     1 LPTDLPLDSVSFLFASAHPSRPA--LIDaatgRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1600 KAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA-NQRALLTGDVPRILLDTADFSHLSED---------NPHVPGLDAH 1669
Cdd:cd05904    79 SLGAVVTTANPLSTPAEIAKQVKDSGAKLAFTTAeLAEKLASLALPVVLLDSAEFDSLSFSdllfeadeaEPPVVVIKQD 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1670 HLAYVIYTSGSTGKPKGVMNSHR---ALCNRLVWMQNTyRLTPDDRVLQKTPFSfdvSVWEFFWPLLYGARL-----VMA 1741
Cdd:cd05904   159 DVAALLYSSGTTGRSKGVMLTHRnliAMVAQFVAGEGS-NSDSEDVFLCVLPMF---HIYGLSSFALGLLRLgatvvVMP 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1742 RpdghKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADcACD--SLRRVICSGEALPAELQQRFFARF-NAQLHNLYG 1818
Cdd:cd05904   235 R----FDLEELLAAIERYKVTHLPVVPPIVLALVKSPIVD-KYDlsSLRQIMSGAAPLGKELIEAFRAKFpNVDLGQGYG 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1819 PTEA-AIDVTFwaCQPDDHRS-FVPIGRPIANTQLYILDT-LGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDP 1895
Cdd:cd05904   310 MTEStGVVAMC--FAPEKDRAkYGSVGRLVPNVEAKIVDPeTGESLPPNQTGELWIRGPSIMKGYLNNPEATAATIDKEG 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1896 FInqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQ 1975
Cdd:cd05904   388 WL--------HTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRK 459

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 641744967 1976 PGVTPDPADLRQQLGQHLAEY----MVpgAFVtlDAFPLTPNGKLDRKAL 2021
Cdd:cd05904   460 PGSSLTEDEIMDFVAKQVAPYkkvrKV--AFV--DAIPKSPSGKILRKEL 505
ACS-like cd17634
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ...
 1504-2016 8.65e-45

acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341289 [Multi-domain]  Cd Length: 587  Bit Score: 173.92  E-value: 8.65e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1504 ILPDSERRQVMLDFNATEADFPHDA---LIHQLVEDQAARTPDTTAVLFED------QHLTYDALNRRANQLAHHLIDLG 1574
Cdd:cd17634    26 ITPYQKVKNTSFAPGAPSIKWFEDAtlnLAANALDRHLRENGDRTAIIYEGddtsqsRTISYRELHREVCRFAGTLLDLG 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1575 VKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLT---------------QANQRALL 1639
Cdd:cd17634   106 VKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLITadggvragrsvplkkNVDDALNP 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1640 TGDVPR--ILLDTA------------DFSHLSEDNP--HVP-GLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVW-M 1701
Cdd:cd17634   186 NVTSVEhvIVLKRTgsdidwqegrdlWWRDLIAKASpeHQPeAMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATtM 265

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFSFDVS-VWEFFWPLLYGARLVM--ARPDgHKDAAYLAQLIERTGITTLHFVPSMLQQfVQWA 1778
Cdd:cd17634   266 KYVFDYGPGDIYWCTADVGWVTGhSYLLYGPLACGATTLLyeGVPN-WPTPARMWQVVDKHGVNILYTAPTAIRA-LMAA 343

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1779 DADCA----CDSLRRVICSGEALPAELQQRFFARFNAQ---LHNLYGPTEaaidvTFWACQPDdHRSFVPIG-----RPI 1846
Cdd:cd17634   344 GDDAIegtdRSSLRILGSVGEPINPEAYEWYWKKIGKEkcpVVDTWWQTE-----TGGFMITP-LPGAIELKagsatRPV 417

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1847 ANTQLYILDTLGQPVPLGVAGELHIGGV--GVARGYLNRPDltaeRFIPDPFINQPGarLYKTGDLARWLPDGSLEYLGR 1924
Cdd:cd17634   418 FGVQPAVVDNEGHPQPGGTEGNLVITDPwpGQTRTLFGDHE----RFEQTYFSTFKG--MYFSGDGARRDEDGYYWITGR 491

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1925 NDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDP---ADLRQQLGQHLAEYMVPGA 2001
Cdd:cd17634   492 SDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPelyAELRNWVRKEIGPLATPDV 571

                         570
                  ....*....|....*
gi 641744967 2002 FVTLDAFPLTPNGKL 2016
Cdd:cd17634   572 VHWVDSLPKTRSGKI 586
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
1518-2021 1.71e-44

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 171.85  E-value: 1.71e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1518 NATEADFPHDalihqlvedQAARTPDTTAVLfeDQH---LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIG 1594
Cdd:PRK06087   22 DASLADYWQQ---------TARAMPDKIAVV--DNHgasYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTII 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1595 LLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALL--TQANQRAL------LTGDVPR----ILLD-------TADFSH 1655
Cdd:PRK06087   91 YLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFFapTLFKQTRPvdlilpLQNQLPQlqqiVGVDklapatsSLSLSQ 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1656 LSEDNP---HVPGLDAHHLAYVIYTSGSTGKPKGVMNSH-------RALCNRLvwmqntyRLTPDDRVLQKTPFSFDVSv 1725
Cdd:PRK06087  171 IIADYEpltTAITTHGDELAAVLFTSGTEGLPKGVMLTHnnilaseRAYCARL-------NLTWQDVFMMPAPLGHATG- 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1726 weFFW----PLLYGARLVMARpdgHKDAAYLAQLIERTGITTLH----FVPSMLQQfVQWADADCAcdSLRRVICSGEAL 1797
Cdd:PRK06087  243 --FLHgvtaPFLIGARSVLLD---IFTPDACLALLEQQRCTCMLgatpFIYDLLNL-LEKQPADLS--ALRFFLCGGTTI 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1798 PAELQQRFFARfNAQLHNLYGPTEAAIDVTfwaCQPDD--HRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVG 1875
Cdd:PRK06087  315 PKKVARECQQR-GIKLLSVYGSTESSPHAV---VNLDDplSRFMHTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRGPN 390

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1876 VARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAV 1955
Cdd:PRK06087  391 VFMGYLDEPELTARALDEEGW--------YYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDAC 462

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 1956 VVAREDSPGDTRLVAYLCPQPGV-TPDPADLRQQLG-QHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK06087  463 VVAMPDERLGERSCAYVVLKAPHhSLTLEEVVAFFSrKRVAKYKYPEHIVVIDKLPRTASGKIQKFLL 530
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
 2631-3097 1.81e-44

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 168.81  E-value: 1.81e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQ 2710
Cdd:cd05935     1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2711 SAhlgimngslpvilLDDgetrpfdnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGI 2790
Cdd:cd05935    81 SE-------------LDD-------------------------LALIPYTSGTTGLPKGCMHTHFSAAANALQSAVWTGL 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2791 AQEDVLLGVTSLsFDIS--ILEIFLPLLNGARLILATQAQAADAQQlamLIERHAVSFMQATPStwrMLVELRDfalPPG 2868
Cdd:cd05935   123 TPSDVILACLPL-FHVTgfVGSLNTAVYVGGTYVLMARWDRETALE---LIEKYKVTFWTNIPT---MLVDLLA---TPE 192

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2869 FKALC---------GGEALPENLATALLQKVTTLWN-LYGPTETTIWSTLNGLTTP-TPYIGHPIANTQIYILDAQ-GRV 2936
Cdd:cd05935   193 FKTRDlsslkvltgGGAPMPPAVAEKLLKLTGLRFVeGYGLTETMSQTHTNPPLRPkLQCLGIP*FGVDARVIDIEtGRE 272

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2937 VPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDpfSGapeARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGE 3016
Cdd:cd05935   273 LPPNEVGEIVVRGPQIFKGYWNRPEETEESFIEI--KG---RRFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAE 347

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3017 IETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLE--PADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDR 3094
Cdd:cd05935   348 VEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYRGKvtEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILW 427


                  ...
gi 641744967 3095 KAL 3097
Cdd:cd05935   428 RLL 430
PRK07798 PRK07798
acyl-CoA synthetase; Validated
 2611-3093 1.03e-43

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 169.30  E-value: 1.03e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2611 LFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTY 2690
Cdd:PRK07798    8 LFEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRY 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2691 PVERLRYMLDDAKPVALISQSAHLGIMNGSLP-------VILLDDGETRPFDNEPDTPLDARKQGLTPRHLA-------Y 2756
Cdd:PRK07798   88 VEDELRYLLDDSDAVALVYEREFAPRVAEVLPrlpklrtLVVVEDGSGNDLLPGAVDYEDALAAGSPERDFGerspddlY 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2757 VIYTSGSTGKPKGVMVEHANM-------VNFLCS--MRKEPGIAQEdVLLGVTSLSFDISIL-------EIFLPLLNGAR 2820
Cdd:PRK07798  168 LLYTGGTTGMPKGVMWRQEDIfrvllggRDFATGepIEDEEELAKR-AAAGPGMRRFPAPPLmhgagqwAAFAALFSGQT 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2821 LILATQAQAADAQQLAmLIERHAVSFMQ------ATPstwrMLVELR---DFALPPGFKALCGGEALPENLATALLQKV- 2890
Cdd:PRK07798  247 VVLLPDVRFDADEVWR-TIEREKVNVITivgdamARP----LLDALEargPYDLSSLFAIASGGALFSPSVKEALLELLp 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2891 -TTLWNLYGPTET----TIWSTLNGLTTPTPYIGhpiANTQIYILDAQGRVVPLGVAGEIHIAGAGVV-RGYLGRPDLTA 2964
Cdd:PRK07798  322 nVVLTDSIGSSETgfggSGTVAKGAVHTGGPRFT---IGPRTVVLDEDGNPVEPGSGEIGWIARRGHIpLGYYKDPEKTA 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2965 ERFITdpFSGapeARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRL 3044
Cdd:PRK07798  399 ETFPT--IDG---VRYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDERWGQEV 473

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967 3045 VAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLD 3093
Cdd:PRK07798  474 VAVVQLREGARPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGKAD 522
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
 2620-3097 1.33e-43

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 168.32  E-value: 1.33e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:cd05959    18 GDKTAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYL 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKP-VALISQSAH------LGIMNGSLPVILLDDGE-----TRPFDNEPDTPLDARKQGLT-PRHLAYVIYTSGSTGK 2766
Cdd:cd05959    98 EDSRArVVVVSGELApvlaaaLTKSEHTLVVLIVSGGAgpeagALLLAELVAAEAEQLKPAAThADDPAFWLYSSGSTGR 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2767 PKGVMVEHANMVnFLCSMRKEP--GIAQEDVLLGVTSLSFDISILE-IFLPLLNGARLILATQAQAADAQQLAMLIERHA 2843
Cdd:cd05959   178 PKGVVHLHADIY-WTAELYARNvlGIREDDVCFSAAKLFFAYGLGNsLTFPLSVGATTVLMPERPTPAAVFKRIRRYRPT 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2844 VSF--------MQATP-STWRMLVELRdfalppgfKALCGGEALPEnlatallqKVTTLW-NLYGPTettiwsTLNGL-T 2912
Cdd:cd05959   257 VFFgvptlyaaMLAAPnLPSRDLSSLR--------LCVSAGEALPA--------EVGERWkARFGLD------ILDGIgS 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2913 TPTPYI--------------GHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFitdpfsgapEA 2978
Cdd:cd05959   315 TEMLHIflsnrpgrvrygttGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKTRDTF---------QG 385

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2979 RMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQP---DTV 3055
Cdd:cd05959   386 EWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRPgyeDSE 465

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|..
gi 641744967 3056 LEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05959   466 ALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKL 507
FUM14_C_NRPS-like cd19545
Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond ...
 2161-2568 1.80e-43

Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond forming Fusarium verticillioides FUM14 protein; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) typically catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. However, some C-domains have ester-bond forming activity. This subfamily includes Fusarium verticillioides FUM14 (also known as NRPS8), a bi-domain protein with an ester-bond forming NRPS C-domain, which catalyzes linkages between an aminoacyl/peptidyl-PCP donor and a hydroxyl-containing acceptor. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. FUM14 has an altered active site motif DHTHCD instead of the typical HHxxxD motif seen in other subfamily members. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380467 [Multi-domain]  Cd Length: 395  Bit Score: 164.78  E-value: 1.80e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2161 IYPLAPLQEGILfhHLLQEQGDTYLLRSmvAFTHRERLD--AFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILP 2238
Cdd:cd19545     1 IYPCTPLQEGLM--ALTARQPGAYVGQR--VFELPPDIDlaRLQAAWEQVVQANPILRTRIVQSDSGGLLQVVVKESPIS 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2239 INHFepTSPEDVLAQlqahtePRTRRIDLSQaPLFRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQAd 2318
Cdd:cd19545    77 WTES--TSLDEYLEE------DRAAPMGLGG-PLVRLALVEDP-DTERYFVWTIHHALYDGWSLPLILRQVLAAYQGEP- 145

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2319 aLPTPLPYRNFIAQTLSVPNSAHEAYFRDKLADVDEPTAP-FGLLNVQGSGGDIHEARLVLDATLASairqqarhlGVSP 2397
Cdd:cd19545   146 -VPQPPPFSRFVKYLRQLDDEAAAEFWRSYLAGLDPAVFPpLPSSRYQPRPDATLEHSISLPSSASS---------GVTL 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2398 GVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADRIMGMFINTLPLRISLADRGA-AEVVERTSHDLMTLLEHEQA 2476
Cdd:cd19545   216 ATVLRAAWALVLSRYTGSDDVVFGVTLSGRNAPVPGIEQIVGPTIATVPLRVRIDPEQSvEDFLQTVQKDLLDMIPFEHT 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2477 PLALAQRCSGVAPPMPLFSTLLNYRHTQASSTDNTLSDIRVLTSEER---TNYPLTLAVDDRGEGFSLVA----QTLEDI 2549
Cdd:cd19545   296 GLQNIRRLGPDARAACNFQTLLVVQPALPSSTSESLELGIEEESEDLedfSSYGLTLECQLSGSGLRVRArydsSVISEE 375

                         410
                  ....*....|....*....
gi 641744967 2550 DPHRLLNYLMTAISSLVDA 2568
Cdd:cd19545   376 QVERLLDQFEHVLQQLASA 394
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
2615-3097 2.12e-43

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 168.77  E-value: 2.12e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2615 QVACTPDAIAVVFGE-ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVE 2693
Cdd:PRK06087   32 TARAMPDKIAVVDNHgASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREA 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2694 RLRYMLDDAKPVALI------------------SQSAHLGimngslPVILLDDgetrpfdNEPDTPLDARKQGLT----- 2750
Cdd:PRK06087  112 ELVWVLNKCQAKMFFaptlfkqtrpvdlilplqNQLPQLQ------QIVGVDK-------LAPATSSLSLSQIIAdyepl 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2751 ----PRH---LAYVIYTSGSTGKPKGVMVEHANMvnfLCSMR---KEPGIAQEDVLLGVTSLSFDISILE-IFLPLLNGA 2819
Cdd:PRK06087  179 ttaiTTHgdeLAAVLFTSGTEGLPKGVMLTHNNI---LASERaycARLNLTWQDVFMMPAPLGHATGFLHgVTAPFLIGA 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2820 RLILATQAQAADAQQlamLIERHAVSF-MQATPSTWRMLVELRdfALPPGFKA----LCGGEALPENLATALLQKVTTLW 2894
Cdd:PRK06087  256 RSVLLDIFTPDACLA---LLEQQRCTCmLGATPFIYDLLNLLE--KQPADLSAlrffLCGGTTIPKKVARECQQRGIKLL 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2895 NLYGPTETTIWSTLNgLTTPTPYIGH----PIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITD 2970
Cdd:PRK06087  331 SVYGSTESSPHAVVN-LDDPLSRFMHtdgyAAAGVEIKVVDEARKTLPPGCEGEEASRGPNVFMGYLDEPELTARALDEE 409

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2971 PFsgapearmYKTGDLGRWLPDGTLEYLGRNDfQVKVRGFR-IELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAY-L 3048
Cdd:PRK06087  410 GW--------YYSGDLCRMDEAGYIKITGRKK-DIIVRGGEnISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYvV 480

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 641744967 3049 LAQPDTVLEPADLRQRLSE-GVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK06087  481 LKAPHHSLTLEEVVAFFSRkRVAKYKYPEHIVVIDKLPRTASGKIQKFLL 530
PRK06178 PRK06178
acyl-CoA synthetase; Validated
 481-963 2.57e-43

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 169.07  E-value: 2.57e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  481 AQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAE 560
Cdd:PRK06178   41 AWARERPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREH 120

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  561 RLAYILDDAAPVALLTQSAQVAQLNSTLPTV----LLDTPAAAACPD--TNPVVQGLHAAH------------------- 615
Cdd:PRK06178  121 ELSYELNDAGAEVLLALDQLAPVVEQVRAETslrhVIVTSLADVLPAepTLPLPDSLRAPRlaaagaidllpalractap 200

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  616 ----------LAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFdasvknWIQ---------L 676
Cdd:PRK06178  201 vplpppaldaLAALNYTGGTTGMPKGCEHTQRDMVYTAAAAYAVAVVGGEDSVFLSFLPEF------WIAgenfgllfpL 274

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  677 LSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSD---PAYQPAQVLIGGEAISPAVWSRLQSLS 753
Cdd:PRK06178  275 FSGATLVLLA---RWDAVAFMAAVERYRVTRTVMLVDNAVELMDHPRFAEydlSSLRQVRVVSFVKKLNPDYRQRWRALT 351

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  754 DTRFINV-YGPTEC-TVDA-TACVVD-----RTQPLpTIGKPLANTRLYILDAQD-QPVPIGVTGELHIGGAGVARGYLH 824
Cdd:PRK06178  352 GSVLAEAaWGMTEThTCDTfTAGFQDddfdlLSQPV-FVGLPVPGTEFKICDFETgELLPLGAEGEIVVRTPSLLKGYWN 430

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  825 RPDLTAERFIpDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDS 904
Cdd:PRK06178  431 KPEATAEALR-DGW--------LHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDP 501

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967  905 PGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVtLDALPLTPNGKLDRKAL 963
Cdd:PRK06178  502 DKGQVPVAFVQLKPGADLTAAALQAWCRENMAVYKVPEIRI-VDALPMTATGKVRKQDL 559
PRK08316 PRK08316
acyl-CoA synthetase; Validated
 483-958 3.68e-43

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 167.42  E-value: 3.68e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:PRK08316   21 ARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEEL 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNPVVQGLHA-------------------AHLAYVIYTS 623
Cdd:PRK08316  101 AYILDHSGARAFLVDPALAPTAEAALALLPVDTLILSLVLGGREAPGGWLDfadwaeagsvaepdveladDDLAQILYTS 180

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  624 GSTGRPKGVMVAHRNVInlATGLHTLLALDH-PSRIALNASIVFD-ASVKNWI--QLLSGHTLVLVPdalRADAHQLWRY 699
Cdd:PRK08316  181 GTESLPKGAMLTHRALI--AEYVSCIVAGDMsADDIPLHALPLYHcAQLDVFLgpYLYVGATNVILD---APDPELILRT 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  700 FARHAVDLFDCTPVqlQWLldaGLGSDPAYQPAQvLIG------GEAISP-AVWSRLQS-LSDTRFINVYGPTECTVDAT 771
Cdd:PRK08316  256 IEAERITSFFAPPT--VWI---SLLRHPDFDTRD-LSSlrkgyyGASIMPvEVLKELRErLPGLRFYNCYGQTEIAPLAT 329

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  772 AcvvdrTQP------LPTIGKPLANTRLYILDAQDQPVPIGVTGELhiggagVAR------GYLHRPDLTAERFIPDPFs 839
Cdd:PRK08316  330 V-----LGPeehlrrPGSAGRPVLNVETRVVDDDGNDVAPGEVGEI------VHRspqlmlGYWDDPEKTAEAFRGGWF- 397

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  840 adpaariyKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPD 919
Cdd:PRK08316  398 --------HSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAG 469

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967  920 AELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKL 958
Cdd:PRK08316  470 ATVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKI 508
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
1530-1992 4.95e-43

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 168.74  E-value: 4.95e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFED----QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAY 1605
Cdd:COG1022    13 LPDLLRRRAARFPDRVALREKEdgiwQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVT 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1606 VPLDPGYPAERLAYMLDDASPVALL--TQANQRALLT--GDVPR----ILLDT-------------------ADFSHLSE 1658
Cdd:COG1022    93 VPIYPTSSAEEVAYILNDSGAKVLFveDQEQLDKLLEvrDELPSlrhiVVLDPrglrddprllsldellalgREVADPAE 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1659 DNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFS--FDvSVWEFFWpLLYGA 1736
Cdd:COG1022   173 LEARRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAhvFE-RTVSYYA-LAAGA 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1737 RLVMARpdghkDAAYLAQLIERTGITTLHFVP-----------------SMLQQFV---------QWADADCACDS---- 1786
Cdd:COG1022   251 TVAFAE-----SPDTLAEDLREVKPTFMLAVPrvwekvyagiqakaeeaGGLKRKLfrwalavgrRYARARLAGKSpsll 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1787 -----------------------LRRVICSGEALPAELqQRFFARFNAQLHNLYGPTEAAIDVTfwaCQPDDHRSFVPIG 1843
Cdd:COG1022   326 lrlkhaladklvfsklrealggrLRFAVSGGAALGPEL-ARFFRALGIPVLEGYGLTETSPVIT---VNRPGDNRIGTVG 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1844 RPIANTQLYILDTlgqpvplgvaGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLG 1923
Cdd:COG1022   402 PPLPGVEVKIAED----------GEILVRGPNVMKGYYKNPEATAEAFDADGW--------LHTGDIGELDEDGFLRITG 463

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967 1924 RNDFQVKLR-GFRIELGEIEARLMQCPGVQEAVVVaredspGDTR--LVAyLcpqpgVTPDPADLRQQLGQH 1992
Cdd:COG1022   464 RKKDLIVTSgGKNVAPQPIENALKASPLIEQAVVV------GDGRpfLAA-L-----IVPDFEALGEWAEEN 523
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
 1536-2020 6.32e-43

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 167.42  E-value: 6.32e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1536 DQAARTPDTTAVLF------EDQHLTYDALNRRANQLAHHLIDLGvKPDDRIAICVERSLDMVIGLLAILKAGAAYVPL- 1608
Cdd:cd05931     1 RRAAARPDRPAYTFlddeggREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLp 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1609 --DPGYPAERLAYMLDDASPVALLTQANQRALLTGDV---------PRILLDTADFShlSEDNPHVPGLDAHHLAYVIYT 1677
Cdd:cd05931    80 ppTPGRHAERLAAILADAGPRVVLTTAAALAAVRAFAasrpaagtpRLLVVDLLPDT--SAADWPPPSPDPDDIAYLQYT 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1678 SGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEF-FWPLLYGARLVMARPdghkdAAYLA--- 1753
Cdd:cd05931   158 SGSTGTPKGVVVTHRNLLANVRQIRRAYGLDPGDVVVSWLPLYHDMGLIGGlLTPLYSGGPSVLMSP-----AAFLRrpl 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1754 ---QLIERTGITTlHFVPSM-LQQFVQWADADCACD----SLRRVICSGEALPAELQQRFFARFNAqlHNL--------Y 1817
Cdd:cd05931   233 rwlRLISRYRATI-SAAPNFaYDLCVRRVRDEDLEGldlsSWRVALNGAEPVRPATLRRFAEAFAP--FGFrpeafrpsY 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1818 GPTEAAIDVTF-----------------------WACQPDDHRSFVPIGRPIANTQLYILDT-LGQPVPLGVAGELHIGG 1873
Cdd:cd05931   310 GLAEATLFVSGgppgtgpvvlrvdrdalagravaVAADDPAARELVSCGRPLPDQEVRIVDPeTGRELPDGEVGEIWVRG 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1874 VGVARGYLNRPDLTAERFIPDPfiNQPGARLYKTGDLARwLPDGSLEYLGRndfqVK----LRGFRIELGEIEARLMQCP 1949
Cdd:cd05931   390 PSVASGYWGRPEATAETFGALA--ATDEGGWLRTGDLGF-LHDGELYITGR----LKdliiVRGRNHYPQDIEATAEEAH 462

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 1950 GVQE---AVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEY--MVPGAFVTL--DAFPLTPNGKLDRKA 2020
Cdd:cd05931   463 PALRpgcVAAFSVPDDGEERLVVVAEVERGADPADLAAIAAAIRAAVAREhgVAPADVVLVrpGSIPRTSSGKIQRRA 540
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 500-963 6.52e-43

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 164.53  E-value: 6.52e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  500 TYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSA 579
Cdd:cd05971     8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVTDGS 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  580 qvaqlnstlptvllDTPAaaacpdtnpvvqglhaahlaYVIYTSGSTGRPKGVMVAHRNVINLATGLHtlLALDHPSRia 659
Cdd:cd05971    88 --------------DDPA--------------------LIIYTSGTTGPPKGALHAHRVLLGHLPGVQ--FPFNLFPR-- 129

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  660 lNASIVFDASVKNWIQLLSGhtlVLVPDAL-----------RADAHQLWRYFARHAVDLFDCTPVQLQWLLDAG-LGSDP 727
Cdd:cd05971   130 -DGDLYWTPADWAWIGGLLD---VLLPSLYfgvpvlahrmtKFDPKAALDLMSRYGVTTAFLPPTALKMMRQQGeQLKHA 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  728 AYQPAQVLIGGEAISPA--VWSRLQsLSDTrfIN-VYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVP 804
Cdd:cd05971   206 QVKLRAIATGGESLGEEllGWAREQ-FGVE--VNeFYGQTECNLVIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLP 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  805 IGVTGELhiggaGVAR-------GYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPDGNIDYLGRNDFQIKVRGFR 877
Cdd:cd05971   283 PGEVGEI-----AVELpdpvaflGYWNNPSATEKKMAGDWL---------LTGDLGRKDSDGYFWYVGRDDDVITSSGYR 348

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  878 IEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARP---DAELHPAALRQQLAASLADYMIPSAFVTLDALPLTP 954
Cdd:cd05971   349 IGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPgetPSDALAREIQELVKTRLAAHEYPREIEFVNELPRTA 428


                  ....*....
gi 641744967  955 NGKLDRKAL 963
Cdd:cd05971   429 TGKIRRREL 437
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
 1530-2021 1.61e-42

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 165.24  E-value: 1.61e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFED-----QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAA 1604
Cdd:PRK08008    9 LRQMWDDLADVYGHKTALIFESsggvvRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAI 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1605 YVPLDPGYPAERLAYMLDDASPVALLTQAN-----QRALLTGDVP--RILL---------DTADFSHLSEDNP----HVP 1664
Cdd:PRK08008   89 MVPINARLLREESAWILQNSQASLLVTSAQfypmyRQIQQEDATPlrHICLtrvalpaddGVSSFTQLKAQQPatlcYAP 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1665 GLDAHHLAYVIYTSGSTGKPKGVMNSHralCNRL------VWmQNtyRLTPDDRVLQKTP-FSFDVSVWEFFWPLLYGAR 1737
Cdd:PRK08008  169 PLSTDDTAEILFTSGTTSRPKGVVITH---YNLRfagyysAW-QC--ALRDDDVYLTVMPaFHIDCQCTAAMAAFSAGAT 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1738 LVM-----ARpdghkdaAYLAQLIE-RTGITtlHFVPS-----MLQQFVQWADADCacdsLRRVICSgeaLPAELQQR-- 1804
Cdd:PRK08008  243 FVLlekysAR-------AFWGQVCKyRATIT--ECIPMmirtlMVQPPSANDRQHC----LREVMFY---LNLSDQEKda 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1805 FFARFNAQLHNLYGPTEAAIDVTfwACQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGV---GVARGYL 1881
Cdd:PRK08008  307 FEERFGVRLLTSYGMTETIVGII--GDRPGDKRRWPSIGRPGFCYEAEIRDDHNRPLPAGEIGEICIKGVpgkTIFKEYY 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1882 NRPDLTAERFIPDPFInqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVARED 1961
Cdd:PRK08008  385 LDPKATAKVLEADGWL--------HTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKD 456

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1962 SPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK08008  457 SIRDEAIKAFVVLNEGETLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
 499-963 5.92e-42

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 161.49  E-value: 5.92e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:cd05935     2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGS 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 AQvaqlnstlptvlldtpaaaacpdtnpvvqglhaAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHpSRI 658
Cdd:cd05935    82 EL---------------------------------DDLALIPYTSGTTGLPKGCMHTHFSAAANALQSAVWTGLTP-SDV 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  659 ALNASIVFdaSVKNWIQLLS-----GHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQ 733
Cdd:cd05935   128 ILACLPLF--HVTGFVGSLNtavyvGGTYVLMA---RWDRETALELIEKYKVTFWTNIPTMLVDLLATPEFKTRDLSSLK 202

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  734 VLIGGEA-ISPAVWSRLQSLSDTRFINVYGPTEcTVDATACVVDRTQPLPTIGKPLANTRLYILDAQD-QPVPIGVTGEL 811
Cdd:cd05935   203 VLTGGGApMPPAVAEKLLKLTGLRFVEGYGLTE-TMSQTHTNPPLRPKLQCLGIP*FGVDARVIDIETgRELPPNEVGEI 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  812 HIGGAGVARGYLHRPDLTAERFIpdpfsADPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCP 891
Cdd:cd05935   282 VVRGPQIFKGYWNRPEETEESFI-----EIKGRRFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHP 356

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967  892 GVQDAVVIAREDSPGDTRLVAYLCARPD--AELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05935   357 AI*EVCVISVPDERVGEEVKAFIVLRPEyrGKVTEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
PRK07787 PRK07787
acyl-CoA synthetase; Validated
 488-966 6.16e-42

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 162.47  E-value: 6.16e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  488 EAIAVLFEDQHLTYRELNRRANQLAHhlialGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDP-AYPAERlAYIL 566
Cdd:PRK07787   15 IADAVRIGGRVLSRSDLAGAATAVAE-----RVAGARRVAVLATPTLATVLAVVGALIAGVPVVPVPPdSGVAER-RHIL 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLtqsAQVAQLNSTLPTVLLDTPAAAACPDTNPvvqglHAAHLAYVIYTSGSTGRPKGVMVahrnvinlatgl 646
Cdd:PRK07787   89 ADSGAQAWL---GPAPDDPAGLPHVPVRLHARSWHRYPEP-----DPDAPALIVYTSGTTGPPKGVVL------------ 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 htllaldhpSRIALNASIvfDASVKNW-----------IQLLSGHTLVL-VPDALRADAHqlwryfARHAVDLfdcTPVQ 714
Cdd:PRK07787  149 ---------SRRAIAADL--DALAEAWqwtaddvlvhgLPLFHVHGLVLgVLGPLRIGNR------FVHTGRP---TPEA 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  715 LQWLLDAG-------------LGSDP----AYQPAQVLIGGEAISPA-VWSRLQSLSDTRFINVYGPTEcTVDATACVVD 776
Cdd:PRK07787  209 YAQALSEGgtlyfgvptvwsrIAADPeaarALRGARLLVSGSAALPVpVFDRLAALTGHRPVERYGMTE-TLITLSTRAD 287

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  777 RTQPLPTIGKPLANTRLYILDAQDQPVPI-GVT-GELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLAR 854
Cdd:PRK07787  288 GERRPGWVGLPLAGVETRLVDEDGGPVPHdGETvGELQVRGPTLFDGYLNRPDATAAAFTADGW--------FRTGDVAV 359

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  855 WLPDGNIDYLGRN--DFqIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAElhPAALRQQLA 932
Cdd:PRK07787  360 VDPDGMHRIVGREstDL-IKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVA--ADELIDFVA 436

                         490       500       510
                  ....*....|....*....|....*....|....
gi 641744967  933 ASLADYMIPSAFVTLDALPLTPNGKLDRKALPAP 966
Cdd:PRK07787  437 QQLSVHKRPREVRFVDALPRNAMGKVLKKQLLSE 470
PRK06164 PRK06164
acyl-CoA synthetase; Validated
 470-953 8.04e-42

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 163.76  E-value: 8.04e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  470 PREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLG------- 542
Cdd:PRK06164    7 PRADTLASLLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLAcarlgat 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  543 ----------------ILKAGAAYVPMDPAYPAERLAYILDDAAPVALLT--QSAQVAQLNSTLP-------TVLLDTPA 597
Cdd:PRK06164   87 viavntryrshevahiLGRGRARWLVVWPGFKGIDFAAILAAVPPDALPPlrAIAVVDDAADATPapapgarVQLFALPD 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  598 AAACPDTNPVVQGLHAAHLAYViyTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRI--ALNASIVFDASVKnWIQ 675
Cdd:PRK06164  167 PAPPAAAGERAADPDAGALLFT--TSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLlaALPFCGVFGFSTL-LGA 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  676 LLSGHTLVLVP--DALRAdAHQLWRYFARHAV---DLFDctpvqlqWLLDAGlGSDPAYQPAQVLiGGEAISPAvWSRLQ 750
Cdd:PRK06164  244 LAGGAPLVCEPvfDAART-ARALRRHRVTHTFgndEMLR-------RILDTA-GERADFPSARLF-GFASFAPA-LGELA 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  751 SLSDTRFI---NVYGPTECTV-----DATACVVDRTQPLPTIGKPLANTRlyILDAQDQPV-PIGVTGELHIGGAGVARG 821
Cdd:PRK06164  313 ALARARGVpltGLYGSSEVQAlvalqPATDPVSVRIEGGGRPASPEARVR--ARDPQDGALlPDGESGEIEIRAPSLMRG 390

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  822 YLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAR 901
Cdd:PRK06164  391 YLDNPDATARALTDDGY--------FRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGA 462

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967  902 EdSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLT 953
Cdd:PRK06164  463 T-RDGKTVPVAFVIPTDGASPDEAGLMAACREALAGFKVPARVQVVEAFPVT 513
Cyc_NRPS cd19535
Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 1103-1391 8.44e-42

Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Cyc (heterocyclization) domains catalyze two separate reactions in the creation of heterocyclized peptide products in nonribosomal peptide synthesis: amide bond formation followed by intramolecular cyclodehydration between a Cys, Ser, or Thr side chain and a carbonyl carbon on the peptide backbone to form a thiazoline, oxazoline, or methyloxazoline ring. Cyc-domains are homologous to standard NRPS Condensation (C) domains. C-domains typically have a conserved HHxxxD motif at the active site; Cyc-domains have an alternative, conserved DxxxxD active site motif, mutation of the aspartate residues in this motif can abolish or diminish condensation activity. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and Cyc-domains. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380458 [Multi-domain]  Cd Length: 423  Bit Score: 160.73  E-value: 8.44e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1103 LDRPALTTALNGLVARHESLRTRFTSiDGQpaQQIDPDTLGFSLSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPL 1182
Cdd:cd19535    37 LDPDRLERAWNKLIARHPMLRAVFLD-DGT--QQILPEVPWYGITVHDLRGLSEEEAEAALEELRERLSHRVLDVERGPL 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1183 IRGQLLQLDDNTHVLlltqhHI-----ISDGWSIGILARELAALYqaalEGSEANLPPLPVQYADYaVWQRQWLQGETLN 1257
Cdd:cd19535   114 FDIRLSLLPEGRTRL-----HLsidllVADALSLQILLRELAALY----EDPGEPLPPLELSFRDY-LLAEQALRETAYE 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1258 DLRDYWR---DQLQGAPAL--LEIPTDRPRPSVQRYAgdqvpFHLDAGQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLS 1332
Cdd:cd19535   184 RARAYWQerlPTLPPAPQLplAKDPEEIKEPRFTRRE-----HRLSAEQWQRLKERARQHGVTPSMVLLTAYAEVLARWS 258

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967 1333 GQDDIVIGTPVANRPR--QELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALDAYAH 1391
Cdd:cd19535   259 GQPRFLLNLTLFNRLPlhPDVNDVVGDFTSLLLLEVDGSEGQSFLERARRLQQQLWEDLDH 319
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
 1523-2019 1.05e-41

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 164.02  E-value: 1.05e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1523 DFPHDALIHqLVEDQAARTPDTTAVLFEDQHLTY---DALNRRAnqlAHHLIDLGVKPDDRIAICVERSLDMVIGLLAIL 1599
Cdd:PRK05605   28 DYGDTTLVD-LYDNAVARFGDRPALDFFGATTTYaelGKQVRRA---AAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVL 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1600 KAGAAYVPLDPGYPAERLAYMLDD-----------ASPVA----------------------LLTQ----------ANQR 1636
Cdd:PRK05605  104 RLGAVVVEHNPLYTAHELEHPFEDhgarvaivwdkVAPTVerlrrttpletivsvnmiaampLLQRlalrlpipalRKAR 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1637 ALLTGDVPrillDTADFSHLSE-------DNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLV----WMQNTY 1705
Cdd:PRK05605  184 AALTGPAP----GTVPWETLVDaaiggdgSDVSHPRPTPDDVALILYTSGTTGKPKGAQLTHRNLFANAAqgkaWVPGLG 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1706 RltPDDRVLQKTPF----------SFDVSVweffwpllyGARLV-MARPDghkdAAYLAQLIERTGITTLHFVPSMLQQF 1774
Cdd:PRK05605  260 D--GPERVLAALPMfhaygltlclTLAVSI---------GGELVlLPAPD----IDLILDAMKKHPPTWLPGVPPLYEKI 324

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1775 VQWADA-DCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVtfwACQP-DDHRSFVPIGRPIANTQLY 1852
Cdd:PRK05605  325 AEAAEErGVDLSGVRNAFSGAMALPVSTVELWEKLTGGLLVEGYGLTETSPII---VGNPmSDDRRPGYVGVPFPDTEVR 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1853 ILD--TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDpfinqpgarLYKTGDLARWLPDGSLEYLGRNDFQVK 1930
Cdd:PRK05605  402 IVDpeDPDETMPDGEEGELLVRGPQVFKGYWNRPEETAKSFLDG---------WFRTGDVVVMEEDGFIRIVDRIKELII 472

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1931 LRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPL 2010
Cdd:PRK05605  473 TGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALDPEGLRAYCREHLTRYKVPRRFYHVDELPR 552


                  ....*....
gi 641744967 2011 TPNGKLDRK 2019
Cdd:PRK05605  553 DQLGKVRRR 561
PRK07514 PRK07514
malonyl-CoA synthase; Validated
 483-963 1.08e-41

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 162.74  E-value: 1.08e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFED-QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAER 561
Cdd:PRK07514   12 AFADRDAPFIETPDgLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAE 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  562 LAYILDDAAPVALLTQSAQ---VAQLNSTLPTVLLDT----------PAAAACPDTNPVVQgLHAAHLAYVIYTSGSTGR 628
Cdd:PRK07514   92 LDYFIGDAEPALVVCDPANfawLSKIAAAAGAPHVETldadgtgsllEAAAAAPDDFETVP-RGADDLAAILYTSGTTGR 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  629 PKGVMVAHRNVINLATGLH---------TLL-ALD----HPSRIALNasivfdasvknwIQLLSGHTLVLVPdalRADAH 694
Cdd:PRK07514  171 SKGAMLSHGNLLSNALTLVdywrftpddVLIhALPifhtHGLFVATN------------VALLAGASMIFLP---KFDPD 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  695 QLWRYFARHAVdlFDCTP---VQLqwLLDAGLGSDpAYQPAQVLIGGEAisPAvwsrlqsLSDT----------RFINVY 761
Cdd:PRK07514  236 AVLALMPRATV--MMGVPtfyTRL--LQEPRLTRE-AAAHMRLFISGSA--PL-------LAEThrefqertghAILERY 301

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  762 GPTEcTVDATACVVDRTQPLPTIGKPLANTRLYILDAQD-QPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsa 840
Cdd:PRK07514  302 GMTE-TNMNTSNPYDGERRAGTVGFPLPGVSLRVTDPETgAELPPGEIGMIEVKGPNVFKGYWRMPEKTAEEFRADGF-- 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  841 dpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAredSP----GDTrLVAYLCA 916
Cdd:PRK07514  379 ------FITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIG---VPhpdfGEG-VTAVVVP 448

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*..
gi 641744967  917 RPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK07514  449 KPGAALDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLL 495
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
 480-958 1.53e-41

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 163.21  E-value: 1.53e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  480 EAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:PRK08314   17 EVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQqECGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNR 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDA-APVALLTQS--AQVAQLNSTL---------------------PTVLLDTPAAAACPDTNPVVQ----- 609
Cdd:PRK08314   97 EEELAHYVTDSgARVAIVGSElaPKVAPAVGNLrlrhvivaqysdylpaepeiaVPAWLRAEPPLQALAPGGVVAwkeal 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  610 ------GLHAAH---LAYVIYTSGSTGRPKGVMVAHRNVINLATGlHTLLALDHPSRIAL---------------NASIV 665
Cdd:PRK08314  177 aaglapPPHTAGpddLAVLPYTSGTTGVPKGCMHTHRTVMANAVG-SVLWSNSTPESVVLavlplfhvtgmvhsmNAPIY 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  666 fdasvknwiqllSGHTLVLVPDALRADAHQLwryFARHAVDLFDCTPVQLQWLLdaglgSDPAYQP------AQVLIGGE 739
Cdd:PRK08314  256 ------------AGATVVLMPRWDREAAARL---IERYRVTHWTNIPTMVVDFL-----ASPGLAErdlsslRYIGGGGA 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  740 AISPAVWSRLQSLSDTRFINVYGPTEcTVDATacvvdRTQP-----LPTIGKPLANTRLYILDAQD-QPVPIGVTGELHI 813
Cdd:PRK08314  316 AMPEAVAERLKELTGLDYVEGYGLTE-TMAQT-----HSNPpdrpkLQCLGIPTFGVDARVIDPETlEELPPGEVGEIVV 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  814 GGAGVARGYLHRPDLTAERFIpdpfSADpAARIYKTGDLARWLPDGN---IDYLGRndfQIKVRGFRIEAGEIESRLLRC 890
Cdd:PRK08314  390 HGPQVFKGYWNRPEATAEAFI----EID-GKRFFRTGDLGRMDEEGYffiTDRLKR---MINASGFKVWPAEVENLLYKH 461

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967  891 PGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAA------LRQQLAAsladYMIPSAFVTLDALPLTPNGKL 958
Cdd:PRK08314  462 PAIQEACVIATPDPRRGETVKAVVVLRPEARGKTTEeeiiawAREHMAA----YKYPRIVEFVDSLPKSGSGKI 531
Condensation pfam00668
Condensation domain; This domain is found in many multi-domain enzymes which synthesize ...
 25-459 1.68e-41

Condensation domain; This domain is found in many multi-domain enzymes which synthesize peptide antibiotics. This domain catalyzes a condensation reaction to form peptide bonds in non- ribosomal peptide biosynthesis. It is usually found to the carboxy side of a phosphopantetheine binding domain (pfam00550). It has been shown that mutations in the HHXXXDG motif abolish activity suggesting this is part of the active site.


Pssm-ID: 395541 [Multi-domain]  Cd Length: 454  Bit Score: 160.96  E-value: 1.68e-41
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    25 VQDIYPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHQPVQVVWRQAPL 104
Cdd:pfam00668    1 VQDEYPLSPAQKRMWFLEKLEPHSSAYNMPAVLKLTGELDPERLEKALQELINRHDALRTVFIRQENGEPVQVILEERPF 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   105 TVNTL---TTTSSDTVPAQLRAATDPSNHRLNLSNAPLLSA---TTAHDpvcgEWLLSLSIHHLISDHITQALIIDEIR- 177
Cdd:pfam00668   81 ELEIIdisDLSESEEEEAIEAFIQRDLQSPFDLEKGPLFRAglfRIAEN----RHHLLLSMHHIIVDGVSLGILLRDLAd 156

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   178 ----LLLEDRPEALPKPlPYRNFIAQILSVPLSE----HEQYFRNRLADIDTPTA-PFDLVDVQGNGEDITEARLSLDSS 248
Cdd:pfam00668  157 lyqqLLKGEPLPLPPKT-PYKDYAEWLQQYLQSEdyqkDAAYWLEQLEGELPVLQlPKDYARPADRSFKGDRLSFTLDED 235

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   249 LADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRlqGNLGADRVMGMFINTLPLRVSLRE-RSVHDVV 327
Cdd:pfam00668  236 TEELLRKLAKAHGTTLNDVLLAAYGLLLSRYTGQDDIVVGTPGSGR--PSPDIERMVGMFVNTLPLRIDPKGgKTFSELI 313

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   328 QATSHELMMLLAHEQAPLALAQQCSQVP---PPLPLFSTLFNYrhsQKDASSQFWEGMRQLSG---------RERTNYPI 395
Cdd:pfam00668  314 KRVQEDLLSAEPHQGYPFGDLVNDLRLPrdlSRHPLFDPMFSF---QNYLGQDSQEEEFQLSEldlsvssviEEEAKYDL 390

                          410       420       430       440       450       460       470
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967   396 TLSVDDLGDGFNLT---------AKTVmgvdpERIVHYMLTAIENLVtsleKTPQQPALSQPILPKSERQQVL 459
Cdd:pfam00668  391 SLTASERGGGLTIKidyntslfdEETI-----ERFAEHFKELLEQAI----AHPSQPLSELDLLSDAEKQKLL 454
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
 1554-2025 1.92e-41

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 160.36  E-value: 1.92e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDA-SPVALLTQ 1632
Cdd:cd05969     1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSeAKVLITTE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1633 AnqralltgdvpriLLDTADfshlsednPHVPgldahhlAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDR 1712
Cdd:cd05969    81 E-------------LYERTD--------PEDP-------TLLHYTSGTTGTPKGVLHVHDAMIFYYFTGKYVLDLHPDDI 132

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1713 V-LQKTPFSFDVSVWEFFWPLLYGARLVMArpDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCA---CDSLR 1788
Cdd:cd05969   133 YwCTADPGWVTGTVYGIWAPWLNGVTNVVY--EGRFDAESWYGIIERVKVTVWYTAPTAIRMLMKEGDELARkydLSSLR 210

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1789 RVICSGEALPAELQQRFFARFNAQLHNLYGPTE-AAIDVTFWACQPDDHRSfvpIGRPIANTQLYILDTLGQPVPLGVAG 1867
Cdd:cd05969   211 FIHSVGEPLNPEAIRWGMEVFGVPIHDTWWQTEtGSIMIANYPCMPIKPGS---MGKPLPGVKAAVVDENGNELPPGTKG 287

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1868 ELHI--GGVGVARGYLNRPdltaERFiPDPFINQpgarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARL 1945
Cdd:cd05969   288 ILALkpGWPSMFRGIWNDE----ERY-KNSFIDG----WYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESAL 358

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1946 MQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALP 2022
Cdd:cd05969   359 MEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDElkeEIINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLK 438


                  ...
gi 641744967 2023 APD 2025
Cdd:cd05969   439 AKE 441
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
 497-963 3.41e-41

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 161.25  E-value: 3.41e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  497 QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLT 576
Cdd:cd05904    31 RALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGAKLAFT 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  577 QSAQVAQLNST-LPTVLLDTPAA---------AACPDTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGL 646
Cdd:cd05904   111 TAELAEKLASLaLPVVLLDSAEFdslsfsdllFEADEAEPPVVVIKQDDVAALLYSSGTTGRSKGVMLTHRNLIAMVAQF 190

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  647 HTLLALDHPSRIALNASI----VFDASVKNWIQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAG 722
Cdd:cd05904   191 VAGEGSNSDSEDVFLCVLpmfhIYGLSSFALGLLRLGATVVVMP---RFDLEELLAAIERYKVTHLPVVPPIVLALVKSP 267

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  723 LGSDpaYQPA---QVLIGGEAISPAVWSR-LQSLSDTRFINVYGPTECTVDATACVVDRTQPLP--TIGKPLANTRLYIL 796
Cdd:cd05904   268 IVDK--YDLSslrQIMSGAAPLGKELIEAfRAKFPNVDLGQGYGMTESTGVVAMCFAPEKDRAKygSVGRLVPNVEAKIV 345

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  797 DAQD-QPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRG 875
Cdd:cd05904   346 DPETgESLPPNQTGELWIRGPSIMKGYLNNPEATAATIDKEGW--------LHTGDLCYIDEDGYLFIVDRLKELIKYKG 417

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  876 FRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPN 955
Cdd:cd05904   418 FQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKPGSSLTEDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPS 497


                  ....*...
gi 641744967  956 GKLDRKAL 963
Cdd:cd05904   498 GKILRKEL 505
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
 2616-3097 3.59e-41

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 160.57  E-value: 3.59e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2616 VACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERL 2695
Cdd:cd05920    25 AARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLALPSHRRSEL 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2696 RYMLDDAKPVALISqsahlgimngslpvilldDGETRPFDNEPDTPLDARKQgltpRHLAYVIYTSGSTGKPKGVMVEHA 2775
Cdd:cd05920   105 SAFCAHAEAVAYIV------------------PDRHAGFDHRALARELAESI----PEVALFLLSGGTTGTPKLIPRTHN 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2776 nmvNFLCSMRKEP---GIAQEDVLLGVTSLS--FDISILEIFLPLLNGARLILATQAQAADAQQlamLIERHAVSFMQAT 2850
Cdd:cd05920   163 ---DYAYNVRASAevcGLDQDTVYLAVLPAAhnFPLACPGVLGTLLAGGRVVLAPDPSPDAAFP---LIEREGVTVTALV 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2851 PSTWRMLVELRDF--ALPPGFKAL-CGGEALPENLATALLQKVT-TLWNLYGPTETTIwsTLNGLTTPTPYI----GHPI 2922
Cdd:cd05920   237 PALVSLWLDAAASrrADLSSLRLLqVGGARLSPALARRVPPVLGcTLQQVFGMAEGLL--NYTRLDDPDEVIihtqGRPM 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2923 -ANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRN 3001
Cdd:cd05920   315 sPDDEIRVVDEEGNPVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPDGF--------YRTGDLVRRTPDGYLVVEGRI 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3002 DFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQpDTVLEPADLRQRLSE-GVAEYMIPSAFVT 3080
Cdd:cd05920   387 KDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVLR-DPPPSAAQLRRFLRErGLAAYKLPDRIEF 465

                         490
                  ....*....|....*..
gi 641744967 3081 LDAFPLTPNGKLDRKAL 3097
Cdd:cd05920   466 VDSLPLTAVGKIDKKAL 482
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
 2608-3097 3.63e-41

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 161.85  E-value: 3.63e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGayVPLD 2687
Cdd:COG1021    27 LGDLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGA--IPVF 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2688 pTYPVER---LRYMLDDAKPVALISQSAHLGIMNGSL------------PVILLDD-GETRPFDNEPDTPLDARKQGLTP 2751
Cdd:COG1021   105 -ALPAHRraeISHFAEQSEAVAYIIPDRHRGFDYRALarelqaevpslrHVLVVGDaGEFTSLDALLAAPADLSEPRPDP 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2752 RHLAYVIYTSGSTGKPKGVMVEHANmvnFLCSMRKEPGIA---QEDVLLGVtsL----SFDISILEIFLPLLNGARLILA 2824
Cdd:COG1021   184 DDVAFFQLSGGTTGLPKLIPRTHDD---YLYSVRASAEICgldADTVYLAA--LpaahNFPLSSPGVLGVLYAGGTVVLA 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2825 TQAQAADAQQlamLIERHAVSF-----------MQATP------STWRMLvelrdfalppgfkaLCGGEALPENLATAL- 2886
Cdd:COG1021   259 PDPSPDTAFP---LIERERVTVtalvpplallwLDAAErsrydlSSLRVL--------------QVGGAKLSPELARRVr 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2887 ------LQKVttlwnlYGPTEttiwstlnGLTTPTPyIGHP---IANTQ---------IYILDAQGRVVPLGVAGEIHIA 2948
Cdd:COG1021   322 palgctLQQV------FGMAE--------GLVNYTR-LDDPeevILTTQgrpispddeVRIVDEDGNPVPPGEVGELLTR 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2949 GAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVH 3028
Cdd:COG1021   387 GPYTIRGYYRAPEHNARAFTPDGF--------YRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVH 458

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3029 DAVVIAREDSPGDKRLVAYLLAQPDTvLEPADLRQRLSE-GVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:COG1021   459 DAAVVAMPDEYLGERSCAFVVPRGEP-LTLAELRRFLRErGLAAFKLPDRLEFVDALPLTAVGKIDKKAL 527
CT_NRPS-like cd19542
Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike ...
 1075-1494 4.74e-41

Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike bacterial NRPS, which typically have specialized terminal thioesterase (TE) domains to cyclize peptide products, many fungal NRPSs employ a terminal condensation-like (CT) domain to produce macrocyclic peptidyl products (e.g. cyclosporine and echinocandin). Domains in this subfamily (which includes both terminal and non-terminal domains) typically have a non-canonical conserved [SN]HxxxDx(14)Y motif at their active site compared to the standard Condensation (C) domain active site motif (HHxxxD). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380464 [Multi-domain]  Cd Length: 401  Bit Score: 157.85  E-value: 4.74e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1075 QRLWFLAQL-DPAASQAYHlpaALHLTGRLDRPALTTALNGLVARHESLRTRF--TSIDGQPAQQIdpdtlgfslsshdL 1151
Cdd:cd19542     8 QEGMLLSQLrSPGLYFNHF---VFDLDSSVDVERLRNAWRQLVQRHDILRTVFveSSAEGTFLQVV-------------L 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1152 RKLDEAARTTRVAELAEQEARARFD----LTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALE 1227
Cdd:cd19542    72 KSLDPPIEEVETDEDSLDALTRDLLddptLFGQPPHRLTLLETSSGEVYLVLRISHALYDGVSLPIILRDLAAAYNGQLL 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1228 GSeanlpplPVQYADYAvwqrQWLQGETLNDLRDYWRDQLQGAPALLEiptdrprPSVQRYAGDQVPFHLDAGQLRRLHA 1307
Cdd:cd19542   152 PP-------APPFSDYI----SYLQSQSQEESLQYWRKYLQGASPCAF-------PSLSPKRPAERSLSSTRRSLAKLEA 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1308 LNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANR--PRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERA 1385
Cdd:cd19542   214 FCASLGVTLASLFQAAWALVLARYTGSRDVVFGYVVSGRdlPVPGIDDIVGPCINTLPVRVKLDPDWTVLDLLRQLQQQY 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1386 LDAYAHQALPFEQVVEILQPARSlsySPIFQVMLSLNNTPAQALTLPDLTLSAVERPQHSTH-FDLSLSLIETENGLNGG 1464
Cdd:cd19542   294 LRSLPHQHLSLREIQRALGLWPS---GTLFNTLVSYQNFEASPESELSGSSVFELSAAEDPTeYPVAVEVEPSGDSLKVS 370

                         410       420       430
                  ....*....|....*....|....*....|
gi 641744967 1465 LVYATDLFDRETILRVVGYVENILMAMADD 1494
Cdd:cd19542   371 LAYSTSVLSEEQAEELLEQFDDILEALLAN 400
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
 616-963 6.47e-41

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 155.18  E-value: 6.47e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  616 LAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSR--IALNASIVFDASVKnWIQLLSGHTLVLvPDALRADA 693
Cdd:cd17630     2 LATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSwlLSLPLYHVGGLAIL-VRSLLAGAELVL-LERNQALA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  694 HQLWRYFARHAvdlfDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRLQSLsDTRFINVYGPTECTVDATAC 773
Cdd:cd17630    80 EDLAPPGVTHV----SLVPTQLQRLLDSGQGPAALKSLRAVLLGGAPIPPELLERAADR-GIPLYTTYGMTETASQVATK 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  774 VVDRTQpLPTIGKPLANTRLYILDaqdqpvpigvTGELHIGGAGVARGYLHRPdltaerfIPDPFSADPaarIYKTGDLA 853
Cdd:cd17630   155 RPDGFG-RGGVGVLLPGRELRIVE----------DGEIWVGGASLAMGYLRGQ-------LVPEFNEDG---WFTTKDLG 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  854 RWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLcaRPDAELHPAALRQQLAA 933
Cdd:cd17630   214 ELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVI--VGRGPADPAELRAWLKD 291

                         330       340       350
                  ....*....|....*....|....*....|
gi 641744967  934 SLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd17630   292 KLARFKLPKRIYPVPELPRTGGGKVDRRAL 321
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
482-963 7.25e-41

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 159.74  E-value: 7.25e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  482 QAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAER 561
Cdd:PRK03640   11 RAFLTPDRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREE 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  562 LAYILDDAAPVALLTQSAQVAQLNSTLPtVLLDTPAAAACPDTNPvVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRN--- 638
Cdd:PRK03640   91 LLWQLDDAEVKCLITDDDFEAKLIPGIS-VKFAELMNGPKEEAEI-QEEFDLDEVATIMYTSGTTGKPKGVIQTYGNhww 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  639 -----VINLatGLH------TLLALDHPSRIalnaSIVFDasvknwiQLLSGHTLVLVPdalRADAHQLWRYFARHAVDL 707
Cdd:PRK03640  169 savgsALNL--GLTeddcwlAAVPIFHISGL----SILMR-------SVIYGMRVVLVE---KFDAEKINKLLQTGGVTI 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  708 FDCTPVQLQWLLdAGLGSDPaYQPA--QVLIGGEAISPAVwsrLQSLSDTRF--INVYGPTE-----CTVDATacvvDRT 778
Cdd:PRK03640  233 ISVVSTMLQRLL-ERLGEGT-YPSSfrCMLLGGGPAPKPL---LEQCKEKGIpvYQSYGMTEtasqiVTLSPE----DAL 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  779 QPLPTIGKPLANTRLYILDaQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPD 858
Cdd:PRK03640  304 TKLGSAGKPLFPCELKIEK-DGVVVPPFEEGEIVVKGPNVTKGYLNREDATRETFQDGWF---------KTGDIGYLDEE 373

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  859 GNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCArpDAELHPAALRQQLAASLADY 938
Cdd:PRK03640  374 GFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFVVK--SGEVTEEELRHFCEEKLAKY 451

                         490       500
                  ....*....|....*....|....*
gi 641744967  939 MIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK03640  452 KVPKRFYFVEELPRNASGKLLRHEL 476
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
 2581-3097 8.20e-41

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 160.99  E-value: 8.20e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2581 PVLPDSERQQMlvdfnaTDADIPRHALIHELFEAQVACTPDAIAVV-----FGEAS-LSYDELNRRANRLAHHLISFGVR 2654
Cdd:PRK13295    5 AVLLPPRRAAS------IAAGHWHDRTINDDLDACVASCPDKTAVTavrlgTGAPRrFTYRELAALVDRVAVGLARLGVG 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2655 PDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQSA-----HLGIMNG---SLP---- 2722
Cdd:PRK13295   79 RGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIFRERELSFMLKHAESKVLVVPKTfrgfdHAAMARRlrpELPalrh 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2723 VILLD-DGE--------TRPFDNEPDTP--LDARKQGltPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIA 2791
Cdd:PRK13295  159 VVVVGgDGAdsfealliTPAWEQEPDAPaiLARLRPG--PDDVTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLG 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2792 QEDVLLGVTSLS----FDISILeifLPLLNGARLILATQAQAADAQQlamLIERHAVSF-MQATPSTWRML--VELRDFA 2864
Cdd:PRK13295  237 ADDVILMASPMAhqtgFMYGLM---MPVMLGATAVLQDIWDPARAAE---LIRTEGVTFtMASTPFLTDLTraVKESGRP 310

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2865 LPPGFKALCGGEALPENLA----TALLQKVTTLWnlyGPTETTIwSTLNGLTTPTPYI----GHPIANTQIYILDAQGRV 2936
Cdd:PRK13295  311 VSSLRTFLCAGAPIPGALVerarAALGAKIVSAW---GMTENGA-VTLTKLDDPDERAsttdGCPLPGVEVRVVDADGAP 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2937 VPLGVAGEIHIAGAGVVRGYLGRPDLTAerfitDPFSGapearMYKTGDLGRWLPDGTLEYLGRNDfQVKVRGFR-IELG 3015
Cdd:PRK13295  387 LPAGQIGRLQVRGCSNFGGYLKRPQLNG-----TDADG-----WFDTGDLARIDADGYIRISGRSK-DVIIRGGEnIPVV 455

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3016 EIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRL-SEGVAEYMIPSAFVTLDAFPLTPNGKLDR 3094
Cdd:PRK13295  456 EIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFEEMVEFLkAQKVAKQYIPERLVVRDALPRTPSGKIQK 535


                  ...
gi 641744967 3095 KAL 3097
Cdd:PRK13295  536 FRL 538
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
 2631-3097 1.70e-40

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 157.76  E-value: 1.70e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQ 2710
Cdd:cd05907     5 PITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVE 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2711 sahlgimngslpvillddgetrpfdnepdtpldarkqglTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGI 2790
Cdd:cd05907    85 ---------------------------------------DPDDLATIIYTSGTTGRPKGVMLSHRNILSNALALAERLPA 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2791 AQEDVLLGVTSLSfdiSILE----IFLPLLNGARLILatqaQAADAQQLAMLIERHAVSFMQAtPSTWRMLVELRDFALP 2866
Cdd:cd05907   126 TEGDRHLSFLPLA---HVFErragLYVPLLAGARIYF----ASSAETLLDDLSEVRPTVFLAV-PRVWEKVYAAIKVKAV 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2867 PGFK--------------ALCGGEALPENLATALLQKVTTLWNLYGPTETTIWSTLNGLTTPTP-YIGHPIANTQIYIld 2931
Cdd:cd05907   198 PGLKrklfdlavggrlrfAASGGAPLPAELLHFFRALGIPVYEGYGLTETSAVVTLNPPGDNRIgTVGKPLPGVEVRI-- 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2932 aqgrvvplGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGR-NDFQVKVRGF 3010
Cdd:cd05907   276 --------ADDGEILVRGPNVMLGYYKNPEATAEALDADGW--------LHTGDLGEIDEDGFLHITGRkKDLIITSGGK 339

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3011 RIELGEIETRLARCHGVHDAVVIaredspGDKR--LVA------------------------YLLAQPDTVLEPADLRQR 3064
Cdd:cd05907   340 NISPEPIENALKASPLISQAVVI------GDGRpfLVAlivpdpealeawaeehgiaytdvaELAANPAVRAEIEAAVEA 413

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967 3065 LSEGVAEYMIPSAFVTL------DAFPLTPNGKLDRKAL 3097
Cdd:cd05907   414 ANARLSRYEQIKKFLLLpepftiENGELTPTLKLKRPVI 452
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
 1554-2021 1.96e-40

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 156.35  E-value: 1.96e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPvalltqa 1633
Cdd:cd05912     2 YTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDV------- 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 nqralltgdvprilldtadfshlsednphvpGLDAhhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRV 1713
Cdd:cd05912    75 -------------------------------KLDD--IATIMYTSGTTGKPKGVQQTFGNHWWSAIGSALNLGLTEDDNW 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1714 LQKTPFsFDVS-VWEFFWPLLYGARLVMARpdgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCAcDSLRRVIC 1792
Cdd:cd05912   122 LCALPL-FHISgLSILMRSVIYGMTVYLVD---KFDAEQVLHLINSGKVTIISVVPTMLQRLLEILGEGYP-NNLRCILL 196

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1793 SGEALPAEL----QQRffarfNAQLHNLYGPTEAAID-VTFwacQPDD-HRSFVPIGRPIANTQLYILDTLGQPvplGVA 1866
Cdd:cd05912   197 GGGPAPKPLleqcKEK-----GIPVYQSYGMTETCSQiVTL---SPEDaLNKIGSAGKPLFPVELKIEDDGQPP---YEV 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1867 GELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLM 1946
Cdd:cd05912   266 GEILLKGPNVTKGYLNRPDATEESFENGWF---------KTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLL 336

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1947 QCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPdpADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05912   337 SHPAIKEAGVVGIPDDKWGQVPVAFVVSERPISE--EELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHEL 409
PRK07514 PRK07514
malonyl-CoA synthase; Validated
 1538-2021 2.29e-40

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 158.50  E-value: 2.29e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFED-QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAER 1616
Cdd:PRK07514   12 AFADRDAPFIETPDgLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAE 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1617 LAYMLDDASP-VALLTQANQRAL--------------LTGDVPRILLDTAdfSHLSEDNPHVPgLDAHHLAYVIYTSGST 1681
Cdd:PRK07514   92 LDYFIGDAEPaLVVCDPANFAWLskiaaaagaphvetLDADGTGSLLEAA--AAAPDDFETVP-RGADDLAAILYTSGTT 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1682 GKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPF--------SFDVSvweffwpLLYGARLV-MARpdghKDAAYL 1752
Cdd:PRK07514  169 GRSKGAMLSHGNLLSNALTLVDYWRFTPDDVLIHALPIfhthglfvATNVA-------LLAGASMIfLPK----FDPDAV 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1753 AQLIERTgiTTLHFVPS----MLQQfvQWADADcACDSLRRVIcSGEA-LPAELQQRFFARFNAQLHNLYGPTEAAIDVT 1827
Cdd:PRK07514  238 LALMPRA--TVMMGVPTfytrLLQE--PRLTRE-AAAHMRLFI-SGSApLLAETHREFQERTGHAILERYGMTETNMNTS 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1828 fwacQP-DDHRSFVPIGRPIANTQLYILD-TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlY 1905
Cdd:PRK07514  312 ----NPyDGERRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMIEVKGPNVFKGYWRMPEKTAEEFRADGF--------F 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1906 KTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQE-AVV-VAREDSpGDTrLVAYLCPQPGVTPDPA 1983
Cdd:PRK07514  380 ITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVEsAVIgVPHPDF-GEG-VTAVVVPKPGAALDEA 457

                         490       500       510
                  ....*....|....*....|....*....|....*...
gi 641744967 1984 DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK07514  458 AILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLL 495
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
 2633-3097 2.44e-40

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 158.95  E-value: 2.44e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2633 SYDELNRRANRLAHHLISFGVRPDERVAIcvergLDM-----VVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVAL 2707
Cdd:cd12119    27 TYAEVAERARRLANALRRLGVKPGDRVAT-----LAWnthrhLELYYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRVV 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2708 ISQSAHLGI---MNGSLP----VILLDDGETRPFDNEPDTP----LDARKQGLTP-----RHLAYVI-YTSGSTGKPKGV 2770
Cdd:cd12119   102 FVDRDFLPLleaIAPRLPtvehVVVMTDDAAMPEPAGVGVLayeeLLAAESPEYDwpdfdENTAAAIcYTSGTTGNPKGV 181

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2771 MVEHANMV--NFLCSMRKEPGIAQEDVLLGVTSLsFDISILEI-FLPLLNGARLILatQAQAADAQQLAMLIERHAVSFM 2847
Cdd:cd12119   182 VYSHRSLVlhAMAALLTDGLGLSESDVVLPVVPM-FHVNAWGLpYAAAMVGAKLVL--PGPYLDPASLAELIEREGVTFA 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2848 QATPSTWRML---VELRDFALPPGFKALCGGEALPENLATALLQKVTTLWNLYGPTETTIWSTLNGLTT---PTPY---- 2917
Cdd:cd12119   259 AGVPTVWQGLldhLEANGRDLSSLRRVVIGGSAVPRSLIEAFEERGVRVIHAWGMTETSPLGTVARPPSehsNLSEdeql 338

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 -----IGHPIANTQIYILDAQGRVVPL-GVA-GEIHIAGAGVVRGYLGRPDlTAERFITDPFsgapearmYKTGDLGRWL 2990
Cdd:cd12119   339 alrakQGRPVPGVELRIVDDDGRELPWdGKAvGELQVRGPWVTKSYYKNDE-ESEALTEDGW--------LRTGDVATID 409

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2991 PDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVA 3070
Cdd:cd12119   410 EDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTAEELLEFLADKVA 489

                         490       500
                  ....*....|....*....|....*..
gi 641744967 3071 EYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd12119   490 KWWLPDDVVFVDEIPKTSTGKIDKKAL 516
PRK06188 PRK06188
acyl-CoA synthetase; Validated
 487-966 3.51e-40

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 158.61  E-value: 3.51e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVAL----CVERSLEMMVGLLgilkAGAAYVPMDPAYPAERL 562
Cdd:PRK06188   26 PDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALlslnRPEVLMAIGAAQL----AGLRRTALHPLGSLDDH 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAP--------------VALLTQSAQVAQLNSTLPT-VLLDTPAAAACPDTNPVVQGLHAAHLAYVIYTSGSTG 627
Cdd:PRK06188  102 AYVLEDAGIstlivdpapfveraLALLARVPSLKHVLTLGPVpDGVDLLAAAAKFGPAPLVAAALPPDIAGLAYTGGTTG 181

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  628 RPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQLLSGHTLVLVPdalRADAHQLWRYFARHAVDL 707
Cdd:PRK06188  182 KPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHAGGAFFLPTLLRGGTVIVLA---KFDPAEVLRAIEEQRITA 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  708 FDCTPVQLQWLLDAGLGSDPAYQPAQ-VLIGGEAISPAvwsRLQSLSDTR---FINVYGPTECTVdaTACVV-----DRT 778
Cdd:PRK06188  259 TFLVPTMIYALLDHPDLRTRDLSSLEtVYYGASPMSPV---RLAEAIERFgpiFAQYYGQTEAPM--VITYLrkrdhDPD 333

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  779 QP--LPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLARWL 856
Cdd:PRK06188  334 DPkrLTSCGRPTPGLRVALLDEDGREVAQGEVGEICVRGPLVMDGYWNRPEETAEAF---------RDGWLHTGDVARED 404

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  857 PDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLA 936
Cdd:PRK06188  405 EDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAAELQAHVKERKG 484

                         490       500       510
                  ....*....|....*....|....*....|
gi 641744967  937 DYMIPSAFVTLDALPLTPNGKLDRKALPAP 966
Cdd:PRK06188  485 SVHAPKQVDFVDSLPLTALGKPDKKALRAR 514
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
 2616-3096 4.66e-40

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 158.56  E-value: 4.66e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2616 VACTPDAIAVVF------GEASLSYDELNRRANRLAHHLISFGvRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPT 2689
Cdd:cd05931     3 AAARPDRPAYTFlddeggREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPP 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2690 YP---VERLRYMLDDAKPVALISQSAHLGIMNGSLPVILLDDGETRPF-DNEPDTPLDA-RKQGLTPRHLAYVIYTSGST 2764
Cdd:cd05931    82 TPgrhAERLAAILADAGPRVVLTTAAALAAVRAFAASRPAAGTPRLLVvDLLPDTSAADwPPPSPDPDDIAYLQYTSGST 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2765 GKPKGVMVEHANMVNFLCSMRKEPGIAQEDVllGVTSLSF--DIS-ILEIFLPLLNGARLILAtqaqaadaqqlamlier 2841
Cdd:cd05931   162 GTPKGVVVTHRNLLANVRQIRRAYGLDPGDV--VVSWLPLyhDMGlIGGLLTPLYSGGPSVLM----------------- 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2842 HAVSFMQAtPSTW-RMLVELR-------DFALppgfkALCGGEALPENLATALLQKVTTLWN------------------ 2895
Cdd:cd05931   223 SPAAFLRR-PLRWlRLISRYRatisaapNFAY-----DLCVRRVRDEDLEGLDLSSWRVALNgaepvrpatlrrfaeafa 296

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2896 -----------LYGPTETTIWSTLNGLTTPTPYI---------------------------GHPIANTQIYILDAQG-RV 2936
Cdd:cd05931   297 pfgfrpeafrpSYGLAEATLFVSGGPPGTGPVVLrvdrdalagravavaaddpaarelvscGRPLPDQEVRIVDPETgRE 376

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2937 VPLGVAGEIHIAGAGVVRGYLGRPDLTAERFitDPFSGAPEARMYKTGDLGRwLPDGTLEYLGRndfqVK----VRGFRI 3012
Cdd:cd05931   377 LPDGEVGEIWVRGPSVASGYWGRPEATAETF--GALAATDEGGWLRTGDLGF-LHDGELYITGR----LKdliiVRGRNH 449

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3013 ELGEIETRLARCHGV---HDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEY--MIPSAFVTL--DAFP 3085
Cdd:cd05931   450 YPQDIEATAEEAHPAlrpGCVAAFSVPDDGEERLVVVAEVERGADPADLAAIAAAIRAAVAREhgVAPADVVLVrpGSIP 529

                         570
                  ....*....|.
gi 641744967 3086 LTPNGKLDRKA 3096
Cdd:cd05931   530 RTSSGKIQRRA 540
PRK07788 PRK07788
acyl-CoA synthetase; Validated
 480-967 6.72e-40

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 158.17  E-value: 6.72e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  480 EAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPA 559
Cdd:PRK07788   56 AHAARRAPDRAALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSG 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  560 ERL----------AYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNPVVQGLHAA-------HLAYVIYT 622
Cdd:PRK07788  136 PQLaevaaregvkALVYDDEFTDLLSALPPDLGRLRAWGGNPDDDEPSGSTDETLDDLIAGSSTAplpkppkPGGIVILT 215

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  623 SGSTGRPKGVMVAHrnvinlATGLHTLLA-LDH-PSR----IALNASIVFDASVKNW-IQLLSGHTLVLvpdALRADAHQ 695
Cdd:PRK07788  216 SGTTGTPKGAPRPE------PSPLAPLAGlLSRvPFRagetTLLPAPMFHATGWAHLtLAMALGSTVVL---RRRFDPEA 286

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  696 LWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQ---VLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATA 772
Cdd:PRK07788  287 TLEDIAKHKATALVVVPVMLSRILDLGPEVLAKYDTSSlkiIFVSGSALSPELATRALEAFGPVLYNLYGSTEVAFATIA 366

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  773 CVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYlhrpdlTAERfipDPFSADPaarIYKTGDL 852
Cdd:PRK07788  367 TPEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGY------TDGR---DKQIIDG---LLSSGDV 434

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  853 ARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLA 932
Cdd:PRK07788  435 GYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGAALDEDAIKDYVR 514

                         490       500       510
                  ....*....|....*....|....*....|....*
gi 641744967  933 ASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPD 967
Cdd:PRK07788  515 DNLARYKVPRDVVFLDELPRNPTGKVLKRELREMD 549
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
 1549-2016 7.41e-40

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 156.99  E-value: 7.41e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1549 FEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVA 1628
Cdd:PRK08276    7 PSGEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKV 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1629 LLTQANQR-------ALLTGDVPRILLDTAD------FSHLSEDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNS----- 1690
Cdd:PRK08276   87 LIVSAALAdtaaelaAELPAGVPLLLVVAGPvpgfrsYEEALAAQPDTPIADETAGADMLYSSGTTGRPKGIKRPlpgld 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1691 -HRALCNRLVWMQNTYRLTPDDRVLQKTPF------SFDVSVweffwpLLYGARLV-MARPDGHKdaaYLAqLIERTGIT 1762
Cdd:PRK08276  167 pDEAPGMMLALLGFGMYGGPDSVYLSPAPLyhtaplRFGMSA------LALGGTVVvMEKFDAEE---ALA-LIERYRVT 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1763 TLHFVPSMLQQFVQWADA-----DCAcdSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEaAIDVTFwaCQPDD-- 1835
Cdd:PRK08276  237 HSQLVPTMFVRMLKLPEEvraryDVS--SLRVAIHAAAPCPVEVKRAMIDWWGPIIHEYYASSE-GGGVTV--ITSEDwl 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1836 -HRSFVpiGRPIAnTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAErfipdpfiNQPGARLYKTGDLArWL 1914
Cdd:PRK08276  312 aHPGSV--GKAVL-GEVRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKTAA--------ARNPHGWVTVGDVG-YL 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1915 -PDGSLeYL-GRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA---DLRQQL 1989
Cdd:PRK08276  380 dEDGYL-YLtDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPADGADAGDAlaaELIAWL 458

                         490       500
                  ....*....|....*....|....*..
gi 641744967 1990 GQHLAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:PRK08276  459 RGRLAHYKCPRSIDFEDELPRTPTGKL 485
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
 499-963 1.09e-39

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 154.80  E-value: 1.09e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:cd05972     1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTDA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 AQVAqlnstlptvlldtpaaaacpdtnpvvqglhaahlaYVIYTSGSTGRPKGVMVAHRnvINLATGLHTLLALD-HPSr 657
Cdd:cd05972    81 EDPA-----------------------------------LIYFTSGTTGLPKGVLHTHS--YPLGHIPTAAYWLGlRPD- 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  658 ialnaSIVFDASVKNWI---------QLLSGHTlVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLgsdPA 728
Cdd:cd05972   123 -----DIHWNIADPGWAkgawssffgPWLLGAT-VFVYEGPRFDAERILELLERYGVTSFCGPPTAYRMLIKQDL---SS 193

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  729 YQPAQ---VLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVdatACVVDRTQPL-P-TIGKPLANTRLYILDAQDQPV 803
Cdd:cd05972   194 YKFSHlrlVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETGL---TVGNFPDMPVkPgSMGRPTPGYDVAIIDDDGREL 270

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  804 PIGVTGEL--HIGGAGVARGYLHRPDLTAERFIPDpfsadpaarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAG 881
Cdd:cd05972   271 PPGEEGDIaiKLPPPGLFLGYVGDPEKTEASIRGD---------YYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPF 341

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  882 EIESRLLRCPGVQDAVVIAREDsPGDTRLV-AYLCARPDAELHPA---ALRQQLAASLADYMIPS--AFVtlDALPLTPN 955
Cdd:cd05972   342 EVESALLEHPAVAEAAVVGSPD-PVRGEVVkAFVVLTSGYEPSEElaeELQGHVKKVLAPYKYPReiEFV--EELPKTIS 418


                  ....*...
gi 641744967  956 GKLDRKAL 963
Cdd:cd05972   419 GKIRRVEL 426
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
 483-962 3.92e-39

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 155.86  E-value: 3.92e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLF------EDQHLTYRELNRRANQLAHHLIALGvQPDDRVALCVERSLEMMVGLLGILKAGAAYVPM--- 553
Cdd:cd05931     3 AAARPDRPAYTFlddeggREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLppp 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  554 DPAYPAERLAYILDDAAPVALLTQSAQVAQL---------NSTLPTVLLDTPAAAACPDTNPVVQGLHAahLAYVIYTSG 624
Cdd:cd05931    82 TPGRHAERLAAILADAGPRVVLTTAAALAAVrafaasrpaAGTPRLLVVDLLPDTSAADWPPPSPDPDD--IAYLQYTSG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  625 STGRPKGVMVAHRNVINLATGLHTLLALDHPSRIA--------------LNASIVFDAS---------VKN---WIQLLS 678
Cdd:cd05931   160 STGTPKGVVVTHRNLLANVRQIRRAYGLDPGDVVVswlplyhdmgliggLLTPLYSGGPsvlmspaafLRRplrWLRLIS 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  679 GHTLVL--VPDalradahqlwryFA-RHAVDLF---DCTPVQLQWLLDAGLGSDP-----------AYQPAqvligG--- 738
Cdd:cd05931   240 RYRATIsaAPN------------FAyDLCVRRVrdeDLEGLDLSSWRVALNGAEPvrpatlrrfaeAFAPF-----Gfrp 302

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  739 EAISPA--------------VWSRLQSLSDTRFINvygptECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQD-QPV 803
Cdd:cd05931   303 EAFRPSyglaeatlfvsggpPGTGPVVLRVDRDAL-----AGRAVAVAADDPAARELVSCGRPLPDQEVRIVDPETgREL 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  804 PIGVTGELHIGGAGVARGYLHRPDLTAERFipDPFSADPAARIYKTGDLARwLPDGNIDYLGRNDFQIKVRGFRIEAGEI 883
Cdd:cd05931   378 PDGEVGEIWVRGPSVASGYWGRPEATAETF--GALAATDEGGWLRTGDLGF-LHDGELYITGRLKDLIIVRGRNHYPQDI 454

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  884 ESRLLRCPGVQD---AVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADY--MIPSAFVTL--DALPLTPNG 956
Cdd:cd05931   455 EATAEEAHPALRpgcVAAFSVPDDGEERLVVVAEVERGADPADLAAIAAAIRAAVAREhgVAPADVVLVrpGSIPRTSSG 534


                  ....*.
gi 641744967  957 KLDRKA 962
Cdd:cd05931   535 KIQRRA 540
PRK06178 PRK06178
acyl-CoA synthetase; Validated
 2619-3097 5.46e-39

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 155.97  E-value: 5.46e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:PRK06178   46 RPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYE 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISQSAHLGIMNG-----------------------SLPV-------ILLDDGETRPFDNEPDTPLDARKQG 2748
Cdd:PRK06178  126 LNDAGAEVLLALDQLAPVVEQvraetslrhvivtsladvlpaepTLPLpdslrapRLAAAGAIDLLPALRACTAPVPLPP 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2749 LTPRHLAYVIYTSGSTGKPKGVMVEHANMV-----NFLCSMRkepgIAQEDVLLGVTSLsFDIS--ILEIFLPLLNGARL 2821
Cdd:PRK06178  206 PALDALAALNYTGGTTGMPKGCEHTQRDMVytaaaAYAVAVV----GGEDSVFLSFLPE-FWIAgeNFGLLFPLFSGATL 280

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2822 ILatqaqaadaqqlamLIERHAVSFMQATP----STWRMLV----ELRDFalpPGFKALcGGEALPENLATALLQKVTT- 2892
Cdd:PRK06178  281 VL--------------LARWDAVAFMAAVEryrvTRTVMLVdnavELMDH---PRFAEY-DLSSLRQVRVVSFVKKLNPd 342

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2893 ---LWN----------LYGPTETtiwSTLNGLTT------------PTpYIGHPIANTQIYILDAQ-GRVVPLGVAGEIH 2946
Cdd:PRK06178  343 yrqRWRaltgsvlaeaAWGMTET---HTCDTFTAgfqdddfdllsqPV-FVGLPVPGTEFKICDFEtGELLPLGAEGEIV 418

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2947 IAGAGVVRGYLGRPDLTAERFitdpfsgapEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHG 3026
Cdd:PRK06178  419 VRTPSLLKGYWNKPEATAEAL---------RDGWLHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPA 489

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967 3027 VHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVtLDAFPLTPNGKLDRKAL 3097
Cdd:PRK06178  490 VLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAAALQAWCRENMAVYKVPEIRI-VDALPMTATGKVRKQDL 559
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
 470-978 6.42e-39

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 155.30  E-value: 6.42e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  470 PREMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAA 549
Cdd:PRK06155   18 PSERTLPAMLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAI 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  550 YVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLNSTLPTV-------LLDTPAAAACP---DTNPVVQGLHAA----- 614
Cdd:PRK06155   98 AVPINTALRGPQLEHILRNSGARLLVVEAALLAALEAADPGDlplpavwLLDAPASVSVPagwSTAPLPPLDAPApaaav 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  615 ---HLAYVIYTSGSTGRPKGVMVAHRNV----INLATGLH--------TLLALDHPSriALNAsiVFDAsvknwiqLLSG 679
Cdd:PRK06155  178 qpgDTAAILYTSGTTGPSKGVCCPHAQFywwgRNSAEDLEigaddvlyTTLPLFHTN--ALNA--FFQA-------LLAG 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  680 HTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVliggeAISPAVWSRLQSLSDTRF-- 757
Cdd:PRK06155  247 ATYVLEP---RFSASGFWPAVRRHGATVTYLLGAMVSILLSQPARESDRAHRVRV-----ALGPGVPAALHAAFRERFgv 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  758 --INVYGPTECTVdatACVVDRTQPLP-TIGKPLANTRLYILDAQDQPVPIGVTGELHIGGA---GVARGYLHRPDLTAE 831
Cdd:PRK06155  319 dlLDGYGSTETNF---VIAVTHGSQRPgSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADepfAFATGYFGMPEKTVE 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  832 rfipdpfsadpAAR--IYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTR 909
Cdd:PRK06155  396 -----------AWRnlWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDE 464

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967  910 LVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAfATRDYEA 978
Cdd:PRK06155  465 VMAAVVLRDGTALEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQGVTA-DTWDREA 532
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
 2624-3097 6.44e-39

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 152.62  E-value: 6.44e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2624 AVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAK 2703
Cdd:cd05919     3 AFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCE 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2704 PVALISQSahlgimngslpvillDDgetrpfdnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVNFLCS 2783
Cdd:cd05919    83 ARLVVTSA---------------DD-------------------------IAYLLYSSGTTGPPKGVMHAHRDPLLFADA 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2784 MRKEP-GIAQEDVLLGVTSLSFDISI-LEIFLPLLNGARLILATQAQAADAQQLamLIERHAVSFMQATPSTWRMLVELR 2861
Cdd:cd05919   123 MAREAlGLTPGDRVFSSAKMFFGYGLgNSLWFPLAVGASAVLNPGWPTAERVLA--TLARFRPTVLYGVPTFYANLLDSC 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2862 DFAlPPGFKAL----CGGEALPENLATALLQkvTTLwnlyGPTETTIWSTLNGLT----TPTPY----IGHPIANTQIYI 2929
Cdd:cd05919   201 AGS-PDALRSLrlcvSAGEALPRGLGERWME--HFG----GPILDGIGATEVGHIflsnRPGAWrlgsTGRPVPGYEIRL 273

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2930 LDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFitdpfsgapEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRG 3009
Cdd:cd05919   274 VDEEGHTIPPGEEGDLLVRGPSAAVGYWNNPEKSRATF---------NGGWYRTGDKFCRDADGWYTHAGRADDMLKVGG 344

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3010 FRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPA---DLRQRLSEGVAEYMIPSAFVTLDAFPL 3086
Cdd:cd05919   345 QWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESlarDIHRHLLERLSAHKVPRRIAFVDELPR 424

                         490
                  ....*....|.
gi 641744967 3087 TPNGKLDRKAL 3097
Cdd:cd05919   425 TATGKLQRFKL 435
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
480-963 9.65e-39

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 153.48  E-value: 9.65e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  480 EAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:PRK06839    9 EKRAYLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLIyELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLT 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTQ------SAQVAQLNSTLPTVLLDTPAAAACPDTNPVVQGLHAAHLaYVIYTSGSTGRPKGV 632
Cdd:PRK06839   89 ENELIFQLKDSGTTVLFVEktfqnmALSMQKVSYVQRVISITSLKEIEDRKIDNFVEKNESASF-IICYTSGTTGKPKGA 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  633 MVAHRNVinLATGLHTLLALDHPSRialNASIVFDASVK-NWIQLLSGHTL-----VLVPDalRADAHQLWRYFARHAVD 706
Cdd:PRK06839  168 VLTQENM--FWNALNNTFAIDLTMH---DRSIVLLPLFHiGGIGLFAFPTLfaggvIIVPR--KFEPTKALSMIEKHKVT 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  707 LFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTEctvdaTACVV------DRTQP 780
Cdd:PRK06839  241 VVMGVPTIHQALINCSKFETTNLQSVRWFYNGGAPCPEELMREFIDRGFLFGQGFGMTE-----TSPTVfmlseeDARRK 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  781 LPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERfIPDPFsadpaariYKTGDLARWLPDGN 860
Cdd:PRK06839  316 VGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRPDATEET-IQDGW--------LCTGDLARVDEDGF 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  861 IDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMI 940
Cdd:PRK06839  387 VYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSVLIEKDVIEHCRLFLAKYKI 466

                         490       500
                  ....*....|....*....|...
gi 641744967  941 PSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK06839  467 PKEIVFLKELPKNATGKIQKAQL 489
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
 1551-2024 2.80e-38

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 152.55  E-value: 2.80e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1551 DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALL 1630
Cdd:PRK12406    9 DRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLI 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1631 TQANQRALLTGDVP------------------RILLD-------TADFSHLSEDNPHVPGLDAHHLAYVIYTSGSTGKPK 1685
Cdd:PRK12406   89 AHADLLHGLASALPagvtvlsvptppeiaaayRISPAlltppagAIDWEGWLAQQEPYDGPPVPQPQSMIYTSGTTGHPK 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1686 GVMNSHRALCNRLVWMQN---TYRLTPDDRVL------QKTPFSFDVSVWEFfwpllyGARLV-MARpdghKDAAYLAQL 1755
Cdd:PRK12406  169 GVRRAAPTPEQAAAAEQMralIYGLKPGIRALltgplyHSAPNAYGLRAGRL------GGVLVlQPR----FDPEELLQL 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1756 IERTGITTLHFVPSMLQQFVQWADADCA---CDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIdVTFwaCQ 1832
Cdd:PRK12406  239 IERHRITHMHMVPTMFIRLLKLPEEVRAkydVSSLRHVIHAAAPCPADVKRAMIEWWGPVIYEYYGSTESGA-VTF--AT 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1833 PDDHRSFvP--IGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVAR-GYLNRPDLTAErfipdpfINQPGarLYKTGD 1909
Cdd:PRK12406  316 SEDALSH-PgtVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGNPDfTYHNKPEKRAE-------IDRGG--FITSGD 385

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1910 LARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQL 1989
Cdd:PRK12406  386 VGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPGATLDEADIRAQL 465

                         490       500       510
                  ....*....|....*....|....*....|....*
gi 641744967 1990 GQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAP 2024
Cdd:PRK12406  466 KARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDP 500
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
 472-963 2.82e-38

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 152.28  E-value: 2.82e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  472 EMLiqqRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYV 551
Cdd:cd05923     5 EML---RRAASRAPDACAIADPARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPA 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  552 PMDPAYPAERLAY-ILDDAAPVALLTQSAQVAQLNSTLPTVLL----DTPAAAACPDTNPV-VQGLHAAHLAYVIYTSGS 625
Cdd:cd05923    82 LINPRLKAAELAElIERGEMTAAVIAVDAQVMDAIFQSGVRVLalsdLVGLGEPESAGPLIeDPPREPEQPAFVFYTSGT 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  626 TGRPKGVMVAHR----NVINLAT------GLHT----LLALDHPS--RIALNASIVFDAsvknwiqllsghTLVLVPDAL 689
Cdd:cd05923   162 TGLPKGAVIPQRaaesRVLFMSTqaglrhGRHNvvlgLMPLYHVIgfFAVLVAALALDG------------TYVVVEEFD 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  690 RADAHQLwryFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLI-GGEAISPAVWSRLQSLSDTRFINVYGPTEctv 768
Cdd:cd05923   230 PADALKL---IEQERVTSLFATPTHLDALAAAAEFAGLKLSSLRHVTfAGATMPDAVLERVNQHLPGEKVNIYGTTE--- 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  769 dATACVVDRTQPLPTIGKPLANTRLY---ILDAQDQPVPIGVTGELHIGGAGVA--RGYLHRPDLTAERFipdpfsadpA 843
Cdd:cd05923   304 -AMNSLYMRDARTGTEMRPGFFSEVRivrIGGSPDEALANGEEGELIVAAAADAafTGYLNQPEATAKKL---------Q 373

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  844 ARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPdAELH 923
Cdd:cd05923   374 DGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPRE-GTLS 452

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 641744967  924 PAALRQ-QLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05923   453 ADELDQfCRASELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
 1538-2021 3.02e-38

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 152.54  E-value: 3.02e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLF--EDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAE 1615
Cdd:PRK13391    7 AQTTPDKPAVIMasTGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPA 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1616 RLAYMLDDASPVALLTQANQR---ALLTGDVP----RILLDTAD-------FSHLSEDNPHVPGLDAHHLAYVIYTSGST 1681
Cdd:PRK13391   87 EAAYIVDDSGARALITSAAKLdvaRALLKQCPgvrhRLVLDGDGelegfvgYAEAVAGLPATPIADESLGTDMLYSSGTT 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1682 GKPKGVMN-----------SHRALCNRLvwmqntYRLTPDDRVLQKTPFSFDVsvweffwPLLY--------GARLVMAr 1742
Cdd:PRK13391  167 GRPKGIKRplpeqppdtplPLTAFLQRL------WGFRSDMVYLSPAPLYHSA-------PQRAvmlvirlgGTVIVME- 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1743 pdgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADA-----DCAcdSLRRVICSGEALPAELQQRFFARFNAQLHNLY 1817
Cdd:PRK13391  233 ---HFDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEvrdkyDLS--SLEVAIHAAAPCPPQVKEQMIDWWGPIIHEYY 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1818 GPTEAaidVTFWACQPDD---HRSFVpiGRPIANTqLYILDTLGQPVPLGVAGELHIGGvGVARGYLNRPDLTAERFIPD 1894
Cdd:PRK13391  308 AATEG---LGFTACDSEEwlaHPGTV--GRAMFGD-LHILDDDGAELPPGEPGTIWFEG-GRPFEYLNDPAKTAEARHPD 380

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1895 PfinqpgaRLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCP 1974
Cdd:PRK13391  381 G-------TWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQP 453

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 641744967 1975 QPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK13391  454 VDGVDPGPAlaaELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLL 503
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
 2602-3097 3.14e-38

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 152.39  E-value: 3.14e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2602 IPRHALIHELFEAQVACTPDAIAVVFGE--ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKA 2679
Cdd:cd05904     1 LPTDLPLDSVSFLFASAHPSRPALIDAAtgRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2680 GGAYVPLDPTYPVERLRYMLDDAKPVALISQSAHLG-IMNGSLPVILLDDGETRPFDNEPDT----PLDARKQGLTPRHL 2754
Cdd:cd05904    81 GAVVTTANPLSTPAEIAKQVKDSGAKLAFTTAELAEkLASLALPVVLLDSAEFDSLSFSDLLfeadEAEPPVVVIKQDDV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2755 AYVIYTSGSTGKPKGVMVEHANMVNFLCS--MRKEPGIAQEDVLLGVTSLsFDISILEIFL--PLLNGARLILATQAQAA 2830
Cdd:cd05904   161 AALLYSSGTTGRSKGVMLTHRNLIAMVAQfvAGEGSNSDSEDVFLCVLPM-FHIYGLSSFAlgLLRLGATVVVMPRFDLE 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2831 DAQQlamLIERHAVSFMQATPStwrMLVELRDFALPPGF------KALCGGEALPENLATALLQKVTT--LWNLYGPTET 2902
Cdd:cd05904   240 ELLA---AIERYKVTHLPVVPP---IVLALVKSPIVDKYdlsslrQIMSGAAPLGKELIEAFRAKFPNvdLGQGYGMTES 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2903 T--IWSTLNGLTTPTPY--IGHPIANTQIYILD-AQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgape 2977
Cdd:cd05904   314 TgvVAMCFAPEKDRAKYgsVGRLVPNVEAKIVDpETGESLPPNQTGELWIRGPSIMKGYLNNPEATAATIDKEGW----- 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2978 armYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLE 3057
Cdd:cd05904   389 ---LHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKPGSSLT 465

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967 3058 PADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05904   466 EDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
PRK06145 PRK06145
acyl-CoA synthetase; Validated
 1538-2021 4.63e-38

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 151.58  E-value: 4.63e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:PRK06145   12 ARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEV 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLTQANQRALLTGDVPRILLDT---ADFSHLSEdnPHVPGLDAH-----HLAYVIYTSGSTGKPKGVMN 1689
Cdd:PRK06145   92 AYILGDAGAKLLLVDEEFDAIVALETPKIVIDAaaqADSRRLAQ--GGLEIPPQAavaptDLVRLMYTSGTTDRPKGVMH 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1690 SHRALCNRLVWMQNTYRLTPDDRVLQKTPF----SFDVSVWEFFWpllYGARLVMARpdgHKDAAYLAQLIERTGITTLH 1765
Cdd:PRK06145  170 SYGNLHWKSIDHVIALGLTASERLLVVGPLyhvgAFDLPGIAVLW---VGGTLRIHR---EFDPEAVLAAIERHRLTCAW 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1766 FVPSMLQQFVQWADAD-CACDSLRRVICSGEALPaELQQRFFARF--NAQLHNLYGPTEAAIDVTFWAcQPDDHRSFVPI 1842
Cdd:PRK06145  244 MAPVMLSRVLTVPDRDrFDLDSLAWCIGGGEKTP-ESRIRDFTRVftRARYIDAYGLTETCSGDTLME-AGREIEKIGST 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1843 GRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlyKTGDLARWLPDGSLEYL 1922
Cdd:PRK06145  322 GRALAHVEIRIADGAGRWLPPNMKGEICMRGPKVTKGYWKDPEKTAEAFYGDWF---------RSGDVGYLDEEGFLYLT 392

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1923 GRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAF 2002
Cdd:PRK06145  393 DRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATLTLEALDRHCRQRLASFKVPRQL 472

                         490
                  ....*....|....*....
gi 641744967 2003 VTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK06145  473 KVRDELPRNPSGKVLKRVL 491
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
 2601-3092 6.74e-38

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 152.04  E-value: 6.74e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2601 DIPRHALIHELfEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLIS-FGVRPDERVAICVERGLDMVVGLLGILKA 2679
Cdd:PRK08314    6 TLPETSLFHNL-EVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQQeCGVRKGDRVLLYMQNSPQFVIAYYAILRA 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2680 GGAYVPLDPTYPVERLRYMLDDAKPVALISQS---------------AHLGIMNGS----------LPVILLDDGETRPF 2734
Cdd:PRK08314   85 NAVVVPVNPMNREEELAHYVTDSGARVAIVGSelapkvapavgnlrlRHVIVAQYSdylpaepeiaVPAWLRAEPPLQAL 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2735 DNEPDTPL-DARKQGLTPR-------HLAYVIYTSGSTGKPKGVMVEHAN-MVNFLCSMRKEpGIAQEDVLLGVTSLsFD 2805
Cdd:PRK08314  165 APGGVVAWkEALAAGLAPPphtagpdDLAVLPYTSGTTGVPKGCMHTHRTvMANAVGSVLWS-NSTPESVVLAVLPL-FH 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2806 ISILEIFL--PLLNGARLILATQAQAADAQQlamLIERHAVSFMQATPStwrMLVelrDFALPPGFKALC---------G 2874
Cdd:PRK08314  243 VTGMVHSMnaPIYAGATVVLMPRWDREAAAR---LIERYRVTHWTNIPT---MVV---DFLASPGLAERDlsslryiggG 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2875 GEALPEnlatALLQKVTTLWNL-----YGPTETTIWSTLNGLTTPTPY-IGHPIANTQIYILDAQ-GRVVPLGVAGEIHI 2947
Cdd:PRK08314  314 GAAMPE----AVAERLKELTGLdyvegYGLTETMAQTHSNPPDRPKLQcLGIPTFGVDARVIDPEtLEELPPGEVGEIVV 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2948 AGAGVVRGYLGRPDLTAERFITdpFSGapeARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGV 3027
Cdd:PRK08314  390 HGPQVFKGYWNRPEATAEAFIE--IDG---KRFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAI 464

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 3028 HDAVVIAREDSPGDKRLVAYLLAQPDTV--LEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:PRK08314  465 QEACVIATPDPRRGETVKAVVVLRPEARgkTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKI 531
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
 448-963 9.10e-38

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 151.74  E-value: 9.10e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  448 PILPKSERQQvlvdfnATDADFPREMLIQQRFEAQAEQTPEAIAVL------FEDQHLTYRELNRRANQLAHHLIALGVQ 521
Cdd:PRK13295    5 AVLLPPRRAA------SIAAGHWHDRTINDDLDACVASCPDKTAVTavrlgtGAPRRFTYRELAALVDRVAVGLARLGVG 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  522 PDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDA-APVALLTQS-------AQVAQLNSTLPTV-- 591
Cdd:PRK13295   79 RGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIFRERELSFMLKHAeSKVLVVPKTfrgfdhaAMARRLRPELPALrh 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  592 --------------LLDTPAAAACPDTNPVVQGLH--AAHLAYVIYTSGSTGRPKGVMVAHrnvinlatglHTLLALDHP 655
Cdd:PRK13295  159 vvvvggdgadsfeaLLITPAWEQEPDAPAILARLRpgPDDVTQLIYTSGTTGEPKGVMHTA----------NTLMANIVP 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  656 --SRIALNA-SIVFDASVknwiqlLSGHT----LVLVPDALRADA--HQLWRyfARHAVDLFDCTPVQLQ-----WLLD- 720
Cdd:PRK13295  229 yaERLGLGAdDVILMASP------MAHQTgfmyGLMMPVMLGATAvlQDIWD--PARAAELIRTEGVTFTmastpFLTDl 300

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  721 AGLGSDPAYQPAQV---LIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPL-PTIGKPLANTRLYIL 796
Cdd:PRK13295  301 TRAVKESGRPVSSLrtfLCAGAPIPGALVERARAALGAKIVSAWGMTENGAVTLTKLDDPDERAsTTDGCPLPGVEVRVV 380

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  797 DAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTaerfipdpfsADPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGF 876
Cdd:PRK13295  381 DADGAPLPAGQIGRLQVRGCSNFGGYLKRPQLN----------GTDADGWFDTGDLARIDADGYIRISGRSKDVIIRGGE 450

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  877 RIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQL-AASLADYMIPSAFVTLDALPLTPN 955
Cdd:PRK13295  451 NIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFEEMVEFLkAQKVAKQYIPERLVVRDALPRTPS 530


                  ....*...
gi 641744967  956 GKLDRKAL 963
Cdd:PRK13295  531 GKIQKFRL 538
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
 499-963 9.57e-38

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 148.65  E-value: 9.57e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPvalltqs 578
Cdd:cd05912     2 YTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDV------- 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 aqvaQLNSTlptvlldtpaaaacpdtnpvvqglhaahlAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSR- 657
Cdd:cd05912    75 ----KLDDI-----------------------------ATIMYTSGTTGKPKGVQQTFGNHWWSAIGSALNLGLTEDDNw 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  658 -IAL------NASIVFDasvknwiQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPaYQ 730
Cdd:cd05912   122 lCALplfhisGLSILMR-------SVIYGMTVYLVD---KFDAEQVLHLINSGKVTIISVVPTMLQRLLEILGEGYP-NN 190

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  731 PAQVLIGGEAISPAVWSRLQSLsDTRFINVYGPTE-CTVDATACVVDRTQPLPTIGKPLANTRLYIldAQDQPVPIGVtG 809
Cdd:cd05912   191 LRCILLGGGPAPKPLLEQCKEK-GIPVYQSYGMTEtCSQIVTLSPEDALNKIGSAGKPLFPVELKI--EDDGQPPYEV-G 266

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  810 ELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLR 889
Cdd:cd05912   267 EILLKGPNVTKGYLNRPDATEESFENGWF---------KTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLS 337

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967  890 CPGVQDAVVIAREDSPGDTRLVAYLCArpDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05912   338 HPAIKEAGVVGIPDDKWGQVPVAFVVS--ERPISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHEL 409
beta-lac_NRPS cd19547
Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis ...
 2161-2532 9.95e-38

Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis NocB which exhibits an unusual cyclization to form beta-lactam rings in pro-nocardicin G synthesis; Nocardia uniformis NRPS NocB acts centrally in the biosynthesis of the nocardicin monocyclic beta-lactam antibiotics. Along with another NRPS NocA, it mediates an unusual cyclization to form beta-lactam rings in the synthesis of the beta-lactam-containing pentapeptide pro-nocardicin G. This small subfamily is related to DCL-type Condensation (C) domains, which catalyze condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; domains belonging to this subfamily have an HHHxxxD motif at the active site.


Pssm-ID: 380469 [Multi-domain]  Cd Length: 422  Bit Score: 149.00  E-value: 9.95e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2161 IYPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVV-------Wr 2233
Cdd:cd19547     1 VYPLAPMQEGMLFRGLFWPDSDAYFNQNVLELVGGTDEDVLREAWRRVADRYEILRTGFTWRDRAEPLQYVrddlappW- 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2234 qAILPINHFEPTSPEDVLAQLQAhtEPRTRRIDLSQAPLFRADIAHDPLQNEWLLaLSFHHLISDHMTLALIVGEI-RL- 2311
Cdd:cd19547    80 -ALLDWSGEDPDRRAELLERLLA--DDRAAGLSLADCPLYRLTLVRLGGGRHYLL-WSHHHILLDGWCLSLIWGDVfRVy 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2312 --LLQHQADALPTPLPYRNFIA--QTLSVPNSAHEAYFRDKLADVdEPTaPFGLLNVQGSgGDIHEARLVLDATLASAIR 2387
Cdd:cd19547   156 eeLAHGREPQLSPCRPYRDYVRwiRARTAQSEESERFWREYLRDL-TPS-PFSTAPADRE-GEFDTVVHEFPEQLTRLVN 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2388 QQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEGADRIMGMFINTLPLRISL-ADRGAAEVVERTSHD 2466
Cdd:cd19547   233 EAARGYGVTTNAISQAAWSMLLALQTGARDVVHGLTIAGRPPELEGSEHMVGIFINTIPLRIRLdPDQTVTGLLETIHRD 312

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967 2467 LMTLLEHEQAPLAL------AQRCSGVAppmpLFSTLLNYR-HTQASSTDNTLS-DIRVLTSEERTNYPLTLAV 2532
Cdd:cd19547   313 LATTAAHGHVPLAQikswasGERLSGGR----VFDNLVAFEnYPEDNLPGDDLSiQIIDLHAQEKTEYPIGLIV 382
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
2619-3097 1.40e-37

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 149.73  E-value: 1.40e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:PRK03640   15 TPDRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELLWQ 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISQSAHLGIMNGSLPVIL--LDDGETRPFDNEPDTPLDArkqgltprhLAYVIYTSGSTGKPKGVMVEHAN 2776
Cdd:PRK03640   95 LDDAEVKCLITDDDFEAKLIPGISVKFaeLMNGPKEEAEIQEEFDLDE---------VATIMYTSGTTGKPKGVIQTYGN 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2777 --------MVNFlcsmrkepGIAQEDVLLGVTSLsFDISILEI-FLPLLNGARLILATQAQAADAQQlamLIERHAVSFM 2847
Cdd:PRK03640  166 hwwsavgsALNL--------GLTEDDCWLAAVPI-FHISGLSIlMRSVIYGMRVVLVEKFDAEKINK---LLQTGGVTII 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2848 QATPSTW-RMLVELRDFALPPGFKA--LCGGEALPENLATAlLQKVTTLWNLYGPTETTiwSTLngLTTPTPYI------ 2918
Cdd:PRK03640  234 SVVSTMLqRLLERLGEGTYPSSFRCmlLGGGPAPKPLLEQC-KEKGIPVYQSYGMTETA--SQI--VTLSPEDAltklgs 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2919 -GHPIANTQIYILDaQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmyKTGDLGRWLPDGTLEY 2997
Cdd:PRK03640  309 aGKPLFPCELKIEK-DGVVVPPFEEGEIVVKGPNVTKGYLNREDATRETFQDGWF---------KTGDIGYLDEEGFLYV 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2998 LGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAqpDTVLEPADLRQRLSEGVAEYMIPSA 3077
Cdd:PRK03640  379 LDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFVVK--SGEVTEEELRHFCEEKLAKYKVPKR 456

                         490       500
                  ....*....|....*....|
gi 641744967 3078 FVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK03640  457 FYFVEELPRNASGKLLRHEL 476
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
 1530-2018 1.42e-37

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 151.46  E-value: 1.42e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHL--TYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVP 1607
Cdd:PRK12583   20 IGDAFDATVARFPDREALVVRHQALryTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVN 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1608 LDPGYPAERLAYMLDDASPVALLTQANQR-----ALLTGDVPRIL------LDTADFSHLS-------EDNPHV------ 1663
Cdd:PRK12583  100 INPAYRASELEYALGQSGVRWVICADAFKtsdyhAMLQELLPGLAegqpgaLACERLPELRgvvslapAPPPGFlawhel 179

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1664 ----------------PGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPF--SFDVsV 1725
Cdd:PRK12583  180 qargetvsrealaerqASLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVPVPLyhCFGM-V 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1726 WEFFWPLLYGARLVMarPDGHKDAAYLAQLIERTGITTLHFVPSMlqqFV------QWADADCAcdSLRRVICSGEALPA 1799
Cdd:PRK12583  259 LANLGCMTVGACLVY--PNEAFDPLATLQAVEEERCTALYGVPTM---FIaeldhpQRGNFDLS--SLRTGIMAGAPCPI 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1800 ELQQRFFARFN-AQLHNLYGPTEAAiDVTFWACQPDD-HRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVA 1877
Cdd:PRK12583  332 EVMRRVMDEMHmAEVQIAYGMTETS-PVSLQTTAADDlERRVETVGRTQPHLEVKVVDPDGATVPRGEIGELCTRGYSVM 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1878 RGYLNRPDLTAERFIPDPFINqpgarlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVV 1957
Cdd:PRK12583  411 KGYWNNPEATAESIDEDGWMH--------TGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVF 482

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967 1958 AREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDR 2018
Cdd:PRK12583  483 GVPDEKYGEEIVAWVRLHPGHAASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQK 543
MACS_like_2 cd05973
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 1554-2021 1.88e-37

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341277 [Multi-domain]  Cd Length: 437  Bit Score: 148.44  E-value: 1.88e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA 1633
Cdd:cd05973     1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTDA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 NQRALLTGDVprilldtadFSHLSednphvpgldahhlayviyTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRv 1713
Cdd:cd05973    81 ANRHKLDSDP---------FVMMF-------------------TSGTTGLPKGVPVPLRALAAFGAYLRDAVDLRPEDS- 131

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1714 lqktpfsfdvsvwefFWPL--------LYGA---RLVMARPDGHKDAAYLAQL----IERTGITTLHFVPSMLQQFVQwA 1778
Cdd:cd05973   132 ---------------FWNAadpgwaygLYYAitgPLALGHPTILLEGGFSVEStwrvIERLGVTNLAGSPTAYRLLMA-A 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1779 DADCACD---SLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDV-TFWACQPDDHRSfvPIGRPIANTQLYIL 1854
Cdd:cd05973   196 GAEVPARpkgRLRRVSSAGEPLTPEVIRWFDAALGVPIHDHYGQTELGMVLaNHHALEHPVHAG--SAGRAMPGWRVAVL 273

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1855 DTLGQPVPLGVAGELHIggvGVAR-------GYLNRPDLTaerfipdpfinqPGARLYKTGDLARWLPDGSLEYLGRNDF 1927
Cdd:cd05973   274 DDDGDELGPGEPGRLAI---DIANsplmwfrGYQLPDTPA------------IDGGYYLTGDTVEFDPDGSFSFIGRADD 338

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1928 QVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDsPGDTRLV-AYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFV 2003
Cdd:cd05973   339 VITMSGYRIGPFDVESALIEHPAVAEAAVIGVPD-PERTEVVkAFVVLRGGHEGTPAladELQLHVKKRLSAHAYPRTIH 417

                         490
                  ....*....|....*...
gi 641744967 2004 TLDAFPLTPNGKLDRKAL 2021
Cdd:cd05973   418 FVDELPKTPSGKIQRFLL 435
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
482-963 4.79e-37

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 149.51  E-value: 4.79e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  482 QAEQTPEAIAVLfeDQH---LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:PRK06087   32 TARAMPDKIAVV--DNHgasYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWR 109

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTQS------------AQVAQLNSTLPTVLLD-------TPAAAACPDTNPVVQG---LHAAHL 616
Cdd:PRK06087  110 EAELVWVLNKCQAKMFFAPTlfkqtrpvdlilPLQNQLPQLQQIVGVDklapatsSLSLSQIIADYEPLTTaitTHGDEL 189

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  617 AYVIYTSGSTGRPKGVMVAHRNVI----NLATGLHtLLALD---HPSriALNASIVFDASVKnwIQLLSGHTLVLvpdal 689
Cdd:PRK06087  190 AAVLFTSGTEGLPKGVMLTHNNILaserAYCARLN-LTWQDvfmMPA--PLGHATGFLHGVT--APFLIGARSVL----- 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  690 radahqLWRYFARHAVDLFD---CTpvqlqWLLDAG---------LGSDPAYQPA-QVLIGGEAISPAVWSRLQSLSDTR 756
Cdd:PRK06087  260 ------LDIFTPDACLALLEqqrCT-----CMLGATpfiydllnlLEKQPADLSAlRFFLCGGTTIPKKVARECQQRGIK 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  757 FINVYGPTECTVDAtacVVDRTQPLP----TIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAER 832
Cdd:PRK06087  329 LLSVYGSTESSPHA---VVNLDDPLSrfmhTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRGPNVFMGYLDEPELTARA 405

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  833 FIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDfQIKVRGFR-IEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLV 911
Cdd:PRK06087  406 LDEEGW--------YYSGDLCRMDEAGYIKITGRKK-DIIVRGGEnISSREVEDILLQHPKIHDACVVAMPDERLGERSC 476

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967  912 AYlcARPDAELHPAALRQQLA----ASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK06087  477 AY--VVLKAPHHSLTLEEVVAffsrKRVAKYKYPEHIVVIDKLPRTASGKIQKFLL 530
FUM14_C_NRPS-like cd19545
Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond ...
 28-435 4.92e-37

Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond forming Fusarium verticillioides FUM14 protein; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) typically catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. However, some C-domains have ester-bond forming activity. This subfamily includes Fusarium verticillioides FUM14 (also known as NRPS8), a bi-domain protein with an ester-bond forming NRPS C-domain, which catalyzes linkages between an aminoacyl/peptidyl-PCP donor and a hydroxyl-containing acceptor. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. FUM14 has an altered active site motif DHTHCD instead of the typical HHxxxD motif seen in other subfamily members. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380467 [Multi-domain]  Cd Length: 395  Bit Score: 145.90  E-value: 4.92e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   28 IYPLAPLQEGiLFHYQLQEKGDtYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHQPVQVVWRQAPLTVN 107
Cdd:cd19545     1 IYPCTPLQEG-LMALTARQPGA-YVGQRVFELPPDIDLARLQAAWEQVVQANPILRTRIVQSDSGGLLQVVVKESPISWT 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  108 TltTTSSDTVPAQLRAATdpsnHRLNlsnAPLLSATTAHDPVCGEWLLsLSIHHLISDHITQALIIDEIRLLLEDRPeaL 187
Cdd:cd19545    79 E--STSLDEYLEEDRAAP----MGLG---GPLVRLALVEDPDTERYFV-WTIHHALYDGWSLPLILRQVLAAYQGEP--V 146

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  188 PKPLPYRNFIAQILSVPLSEHEQYFRNRLADIDTPTAPfdlvdvqgngeditEARLSLDSSLADALRRQARHL------G 261
Cdd:cd19545   147 PQPPPFSRFVKYLRQLDDEAAAEFWRSYLAGLDPAVFP--------------PLPSSRYQPRPDATLEHSISLpssassG 212

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  262 ISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRlqgNL---GADRVMGMFINTLPLRVSL-RERSVHDVVQATSHELMML 337
Cdd:cd19545   213 VTLATVLRAAWALVLSRYTGSDDVVFGVTLSGR---NApvpGIEQIVGPTIATVPLRVRIdPEQSVEDFLQTVQKDLLDM 289

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  338 LAHEQAPLALAQQCSQVPPPLPLFSTLFN--YRHSQKDASSQFWEGMRQLSGRER-TNYPITLSVDDLGDGFNLTA---K 411
Cdd:cd19545   290 IPFEHTGLQNIRRLGPDARAACNFQTLLVvqPALPSSTSESLELGIEEESEDLEDfSSYGLTLECQLSGSGLRVRArydS 369

                         410       420
                  ....*....|....*....|....*
gi 641744967  412 TVMGVDP-ERIVHYMLTAIENLVTS 435
Cdd:cd19545   370 SVISEEQvERLLDQFEHVLQQLASA 394
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
 499-967 8.84e-37

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 146.49  E-value: 8.84e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDA-APVALLTQ 577
Cdd:cd05969     1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSeAKVLITTE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  578 SAqvaqLNSTLPtvllDTPAaaacpdtnpvvqglhaahlaYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALdHPSR 657
Cdd:cd05969    81 EL----YERTDP----EDPT--------------------LLHYTSGTTGTPKGVLHVHDAMIFYYFTGKYVLDL-HPDD 131

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  658 I---ALNASIVFDASVKNWIQLLSGHTLVLVPDalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQ- 733
Cdd:cd05969   132 IywcTADPGWVTGTVYGIWAPWLNGVTNVVYEG--RFDAESWYGIIERVKVTVWYTAPTAIRMLMKEGDELARKYDLSSl 209

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  734 --VLIGGEAISPAV--WSrlQSLSDTRFINVYGPTECTVDATA-CVVDRTQPlPTIGKPLANTRLYILDAQDQPVPIGVT 808
Cdd:cd05969   210 rfIHSVGEPLNPEAirWG--MEVFGVPIHDTWWQTETGSIMIAnYPCMPIKP-GSMGKPLPGVKAAVVDENGNELPPGTK 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  809 GELHI--GGAGVARGYLHRPDLTAERFIPDpfsadpaarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESR 886
Cdd:cd05969   287 GILALkpGWPSMFRGIWNDEERYKNSFIDG---------WYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESA 357

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  887 LLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAA-------LRQQLAASLAdymiPSAFVTLDALPLTPNGKLD 959
Cdd:cd05969   358 LMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELkeeiinfVRQKLGAHVA----PREIEFVDNLPKTRSGKIM 433


                  ....*...
gi 641744967  960 RKALPAPD 967
Cdd:cd05969   434 RRVLKAKE 441
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 2633-3097 1.12e-36

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 146.04  E-value: 1.12e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2633 SYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISqsa 2712
Cdd:cd05971     8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT--- 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2713 hlgimngslpvillddgetrpfdNEPDTPldarkqgltprhlAYVIYTSGSTGKPKG------VMVEHANMVNFLCSMRK 2786
Cdd:cd05971    85 -----------------------DGSDDP-------------ALIIYTSGTTGPPKGalhahrVLLGHLPGVQFPFNLFP 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2787 EPGiaqeDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELRDFALP 2866
Cdd:cd05971   129 RDG----DLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRMTKFDPKAALDLMSRYGVTTAFLPPTALKMMRQQGEQLKH 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2867 PGFKAL---CGGEALPEN-LATALLQKVTTLWNLYGPTETT-IWSTLNGLTTPTP-YIGHPIANTQIYILDAQGRVVPLG 2940
Cdd:cd05971   205 AQVKLRaiaTGGESLGEElLGWAREQFGVEVNEFYGQTECNlVIGNCSALFPIKPgSMGKPIPGHRVAIVDDNGTPLPPG 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2941 VAGEIHIAGAGVVR--GYLGRPDLTAERFITDPFsgapearmyKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIE 3018
Cdd:cd05971   285 EVGEIAVELPDPVAflGYWNNPSATEKKMAGDWL---------LTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIE 355

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3019 TRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPA---DLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRK 3095
Cdd:cd05971   356 ECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDAlarEIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRR 435


                  ..
gi 641744967 3096 AL 3097
Cdd:cd05971   436 EL 437
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
 2632-3097 1.42e-36

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 145.56  E-value: 1.42e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQS 2711
Cdd:cd05972     1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTDA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2712 ahlgimngslpvillDDgetrpfdnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIA 2791
Cdd:cd05972    81 ---------------ED-------------------------PALIYFTSGTTGLPKGVLHTHSYPLGHIPTAAYWLGLR 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2792 QEDVLLGVTSLSFDISIL-EIFLPLLNGARLILATQAQAADAQQLAmLIERHAVSFMQATPSTWRMLVELrdfaLPPGFK 2870
Cdd:cd05972   121 PDDIHWNIADPGWAKGAWsSFFGPWLLGATVFVYEGPRFDAERILE-LLERYGVTSFCGPPTAYRMLIKQ----DLSSYK 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2871 ------ALCGGEAL-PENLATALLQKVTTLWNLYGPTETTIW-STLNGLTTPTPYIGHPIANTQIYILDAQGRVVPLGVA 2942
Cdd:cd05972   196 fshlrlVVSAGEPLnPEVIEWWRAATGLPIRDGYGQTETGLTvGNFPDMPVKPGSMGRPTPGYDVAIIDDDGRELPPGEE 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2943 GEI--HIAGAGVVRGYLGRPDLTAERFITDpfsgapearMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETR 3020
Cdd:cd05972   276 GDIaiKLPPPGLFLGYVGDPEKTEASIRGD---------YYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESA 346

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3021 LARCHGVHDAVVIAredSPGDKR---------LVAYLLAQPDTVLEPADL-RQRLSEGVAEYMIpsAFVtlDAFPLTPNG 3090
Cdd:cd05972   347 LLEHPAVAEAAVVG---SPDPVRgevvkafvvLTSGYEPSEELAEELQGHvKKVLAPYKYPREI--EFV--EELPKTISG 419


                  ....*..
gi 641744967 3091 KLDRKAL 3097
Cdd:cd05972   420 KIRRVEL 426
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
 489-958 1.71e-36

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 146.97  E-value: 1.71e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  489 AIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDD 568
Cdd:PRK08276    2 AVIMAPSGEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDD 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  569 AAPVALLTQ---SAQVAQLNSTLP---TVLLDTP-----------AAAACPDTNPVVQGLhAAHLAyviYTSGSTGRPKG 631
Cdd:PRK08276   82 SGAKVLIVSaalADTAAELAAELPagvPLLLVVAgpvpgfrsyeeALAAQPDTPIADETA-GADML---YSSGTTGRPKG 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  632 VMVA--HRNVINLATGLHTLLALDH---PSRIALNASIVFDASVKNWIQ--LLSGHTLVLVPdalRADAHQLWRYFARHA 704
Cdd:PRK08276  158 IKRPlpGLDPDEAPGMMLALLGFGMyggPDSVYLSPAPLYHTAPLRFGMsaLALGGTVVVME---KFDAEEALALIERYR 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  705 VDLFDCTPVQLQWLLdaglgsdpayqpaqvliggeAISPAVWSRLqSLSDTRF----------------INVYGPT---- 764
Cdd:PRK08276  235 VTHSQLVPTMFVRML--------------------KLPEEVRARY-DVSSLRVaihaaapcpvevkramIDWWGPIihey 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  765 -ECTVDATACVVDRTQPL--P-TIGKPLAnTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSA 840
Cdd:PRK08276  294 yASSEGGGVTVITSEDWLahPgSVGKAVL-GEVRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKTAAARNPHGWVT 372

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  841 dpaariykTGDLArWLPDGNIDYL-GRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPD 919
Cdd:PRK08276  373 --------VGDVG-YLDEDGYLYLtDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPADG 443

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|..
gi 641744967  920 AELHPA---ALRQQLAASLADYMIPSAFVTLDALPLTPNGKL 958
Cdd:PRK08276  444 ADAGDAlaaELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKL 485
PRK06164 PRK06164
acyl-CoA synthetase; Validated
 2603-3087 2.68e-36

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 147.20  E-value: 2.68e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2603 PRHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGA 2682
Cdd:PRK06164    7 PRADTLASLLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGAT 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2683 YVPLDPTYPVERLRYMLDDAKPVALISQSAHLGI-----MNGSLP--------VILLDDGETR-----PFD--NEPDTPL 2742
Cdd:PRK06164   87 VIAVNTRYRSHEVAHILGRGRARWLVVWPGFKGIdfaaiLAAVPPdalpplraIAVVDDAADAtpapaPGArvQLFALPD 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2743 DARKQGLTPRH-----LAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLS--FDISILEIFLPl 2815
Cdd:PRK06164  167 PAPPAAAGERAadpdaGALLFTTSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCgvFGFSTLLGALA- 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2816 lNGARLILATQAQAADAQQlamLIERHAVSFMQATPSTWRMLVEL----RDFALPP--GFKALCGGEAlpeNLATALLQK 2889
Cdd:PRK06164  246 -GGAPLVCEPVFDAARTAR---ALRRHRVTHTFGNDEMLRRILDTagerADFPSARlfGFASFAPALG---ELAALARAR 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2890 VTTLWNLYGPTETTIWSTLNGLTTPTPYI----GHPI-ANTQIYILDAQ-GRVVPLGVAGEIHIAGAGVVRGYLGRPDLT 2963
Cdd:PRK06164  319 GVPLTGLYGSSEVQALVALQPATDPVSVRieggGRPAsPEARVRARDPQdGALLPDGESGEIEIRAPSLMRGYLDNPDAT 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2964 AERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREdSPGDKR 3043
Cdd:PRK06164  399 ARALTDDGY--------FRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGAT-RDGKTV 469

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 641744967 3044 LVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLT 3087
Cdd:PRK06164  470 PVAFVIPTDGASPDEAGLMAACREALAGFKVPARVQVVEAFPVT 513
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
2613-3097 3.71e-36

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 145.77  E-value: 3.71e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2613 EAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLI-SFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYP 2691
Cdd:PRK06839    9 EKRAYLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLIyELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLT 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2692 VERLRYMLDDAKPVALISQ------SAHLGIMNGSLPVILLDDgetrpfdnePDTPLDARKQGLTPRH--LAYVI-YTSG 2762
Cdd:PRK06839   89 ENELIFQLKDSGTTVLFVEktfqnmALSMQKVSYVQRVISITS---------LKEIEDRKIDNFVEKNesASFIIcYTSG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2763 STGKPKGVMVEHANMvnFLCSMRKEPGI--AQEDVLLGVTSLsFDISILEIF-LP-LLNGARLILATQAQAADAQQlamL 2838
Cdd:PRK06839  160 TTGKPKGAVLTQENM--FWNALNNTFAIdlTMHDRSIVLLPL-FHIGGIGLFaFPtLFAGGVIIVPRKFEPTKALS---M 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2839 IERHAVSFMQATPSTWRMLVELRDFAlPPGFKAL----CGGEALPENLATALLQKVTTLWNLYGPTET--TIWSTLNGLT 2912
Cdd:PRK06839  234 IEKHKVTVVMGVPTIHQALINCSKFE-TTNLQSVrwfyNGGAPCPEELMREFIDRGFLFGQGFGMTETspTVFMLSEEDA 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2913 TPTP-YIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERfITDPFsgapearmYKTGDLGRWLP 2991
Cdd:PRK06839  313 RRKVgSIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRPDATEET-IQDGW--------LCTGDLARVDE 383

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2992 DGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAE 3071
Cdd:PRK06839  384 DGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSVLIEKDVIEHCRLFLAK 463

                         490       500
                  ....*....|....*....|....*.
gi 641744967 3072 YMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK06839  464 YKIPKEIVFLKELPKNATGKIQKAQL 489
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
 1671-2021 3.73e-36

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 141.31  E-value: 3.73e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1671 LAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPfSFDVSVWEFFWP-LLYGARLVMARPDghkda 1749
Cdd:cd17630     2 LATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLP-LYHVGGLAILVRsLLAGAELVLLERN----- 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1750 AYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARfNAQLHNLYGPTEAAIDVTFW 1829
Cdd:cd17630    76 QALAEDLAPPGVTHVSLVPTQLQRLLDSGQGPAALKSLRAVLLGGAPIPPELLERAADR-GIPLYTTYGMTETASQVATK 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1830 ACQPDDHRSfvpIGRPIANTQLYILDTlgqpvplgvaGELHIGGVGVARGYLNRPdltaerfIPDPFINQPgarLYKTGD 1909
Cdd:cd17630   155 RPDGFGRGG---VGVLLPGRELRIVED----------GEIWVGGASLAMGYLRGQ-------LVPEFNEDG---WFTTKD 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1910 LARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGvtPDPADLRQQL 1989
Cdd:cd17630   212 LGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGP--ADPAELRAWL 289

                         330       340       350
                  ....*....|....*....|....*....|..
gi 641744967 1990 GQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd17630   290 KDKLARFKLPKRIYPVPELPRTGGGKVDRRAL 321
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
 1667-2021 4.76e-36

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 145.55  E-value: 4.76e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1667 DAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTP----FSFDVSVWeffWPLLYGARLVMAr 1742
Cdd:cd05909   145 QPDDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPffhsFGLTGCLW---LPLLSGIKVVFH- 220

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1743 PDGhKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCAcDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEA 1822
Cdd:cd05909   221 PNP-LDYKKIPELIYDKKATILLGTPTFLRGYARAAHPEDF-SSLRLVVAGAEKLKDTLRQEFQEKFGIRILEGYGTTEC 298

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1823 AIDVTFWACQPDDHRSFVpiGRPIANTQLYILDTLG-QPVPLGVAGELHIGGVGVARGYLNRPDLTAERFipdpfinqpG 1901
Cdd:cd05909   299 SPVISVNTPQSPNKEGTV--GRPLPGMEVKIVSVEThEEVPIGEGGLLLVRGPNVMLGYLNEPELTSFAF---------G 367

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1902 ARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQ-CPGVQEAVVVARED-SPGDtRLVAYLCPQpgvT 1979
Cdd:cd05909   368 DGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEiLPEDNEVAVVSVPDgRKGE-KIVLLTTTT---D 443

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|...
gi 641744967 1980 PDPADLRQQLGQH-LAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05909   444 TDPSSLNDILKNAgISNLAKPSYIHQVEEIPLLGTGKPDYVTL 486
PRK09088 PRK09088
acyl-CoA synthetase; Validated
 1537-2021 7.14e-36

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 144.95  E-value: 7.14e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1537 QAARTPDTTAV--LFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPA 1614
Cdd:PRK09088    4 HARLQPQRLAAvdLALGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSA 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1615 ERLAYMLDDASPVALLTQANQRALLTgdvprILLDTADF--SHLSEDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNSHR 1692
Cdd:PRK09088   84 SELDALLQDAEPRLLLGDDAVAAGRT-----DVEDLAAFiaSADALEPADTPSIPPERVSLILFTSGTSGQPKGVMLSER 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1693 ALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLY--GARLVmarPDGHKDAAYLAQLIERT-GITTLHFVPS 1769
Cdd:PRK09088  159 NLQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAvgGSILV---SNGFEPKRTLGRLGDPAlGITHYFCVPQ 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1770 MLQQFVQWADADCAcdSLRRV--ICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIdVTFWACQPDDHRSFV-PIGRPI 1846
Cdd:PRK09088  236 MAQAFRAQPGFDAA--ALRHLtaLFTGGAPHAAEDILGWLDDGIPMVDGFGMSEAGT-VFGMSVDCDVIRAKAgAAGIPT 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1847 ANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGRND 1926
Cdd:PRK09088  313 PTVQTRVVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAFTGDGW--------FRTGDIARRDADGFFWVVDRKK 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1927 FQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLD 2006
Cdd:PRK09088  385 DMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDLERIRSHLSTRLAKYKVPKHLRLVD 464

                         490
                  ....*....|....*
gi 641744967 2007 AFPLTPNGKLDRKAL 2021
Cdd:PRK09088  465 ALPRTASGKLQKARL 479
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
 496-963 7.29e-36

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 143.77  E-value: 7.29e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  496 DQHLTYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAapval 574
Cdd:cd05958     8 EREWTYRDLLALANRIANVLVgELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKA----- 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  575 ltqsaqvaqlnstlptvlldTPAAAACPDtnpvvQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHT-LLALD 653
Cdd:cd05958    83 --------------------RITVALCAH-----ALTASDDICILAFTSGTTGAPKATMHFHRDPLASADRYAVnVLRLR 137

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  654 HPSRI----------ALNASIVFDASVknwiqllsGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQwlldAGL 723
Cdd:cd05958   138 EDDRFvgspplaftfGLGGVLLFPFGV--------GASGVLLE---EATPDLLLSAIARYKPTVLFTAPTAYR----AML 202

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  724 GSDPAYQP-----AQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPlPTIGKPLANTRLYILDA 798
Cdd:cd05958   203 AHPDAAGPdlsslRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEMFHIFISARPGDARP-GATGKPVPGYEAKVVDD 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  799 QDQPVPIGVTGELHIGGAgvaRGYLHRPDLTAERFIPDPFSAdpaariykTGDLARWLPDGNIDYLGRNDFQIKVRGFRI 878
Cdd:cd05958   282 EGNPVPDGTIGRLAVRGP---TGCRYLADKRQRTYVQGGWNI--------TGDTYSRDPDGYFRHQGRSDDMIVSGGYNI 350

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  879 EAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPA---ALRQQLAASLADYMIPSAFVTLDALPLTPN 955
Cdd:cd05958   351 APPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVlarELQDHAKAHIAPYKYPRAIEFVTELPRTAT 430


                  ....*...
gi 641744967  956 GKLDRKAL 963
Cdd:cd05958   431 GKLQRFAL 438
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
 479-961 7.63e-36

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 146.30  E-value: 7.63e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  479 FEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:PRK05605   38 YDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVVVEHNPLYT 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDD-AAPVA--------LLTQSAQVAQLN--------STLPTVL-----LDTPAA--------AACPDTNP-- 606
Cdd:PRK05605  118 AHELEHPFEDhGARVAivwdkvapTVERLRRTTPLEtivsvnmiAAMPLLQrlalrLPIPALrkaraaltGPAPGTVPwe 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  607 -------VVQGLHAAH-------LAYVIYTSGSTGRPKGVMVAHRNVI-NLATGLHTLLAL-DHPSRIaLNASIVFDA-- 668
Cdd:PRK05605  198 tlvdaaiGGDGSDVSHprptpddVALILYTSGTTGKPKGAQLTHRNLFaNAAQGKAWVPGLgDGPERV-LAALPMFHAyg 276

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  669 -SVKNWIQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLI-GGEAISPAVW 746
Cdd:PRK05605  277 lTLCLTLAVSIGGELVLLP---APDIDLILDAMKKHPPTWLPGVPPLYEKIAEAAEERGVDLSGVRNAFsGAMALPVSTV 353

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  747 SRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQD--QPVPIGVTGELHIGGAGVARGYLH 824
Cdd:PRK05605  354 ELWEKLTGGLLVEGYGLTETSPIIVGNPMSDDRRPGYVGVPFPDTEVRIVDPEDpdETMPDGEEGELLVRGPQVFKGYWN 433

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  825 RPDLTAERFIPDpfsadpaarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDS 904
Cdd:PRK05605  434 RPEETAKSFLDG---------WFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPRE 504

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967  905 PGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRK 961
Cdd:PRK05605  505 DGSEEVVAAVVLEPGAALDPEGLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRR 561
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
1530-2021 3.18e-35

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 143.87  E-value: 3.18e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQH--LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVP 1607
Cdd:PRK05852   18 IADLVEVAATRLPEAPALVVTADRiaISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVP 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1608 LDPGYPAERLAYMLDDASPVALLTQAN---QRALLTGDVPRILLDTADFSHLSEDNPHVPgLDA---------------H 1669
Cdd:PRK05852   98 LDPALPIAEQRVRSQAAGARVVLIDADgphDRAEPTTRWWPLTVNVGGDSGPSGGTLSVH-LDAateptpatstpeglrP 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1670 HLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDA 1749
Cdd:PRK05852  177 DDAMIMFTGGTTGLPKMVPWTHANIASSVRAIITGYRLSPRDATVAVMPLYHGHGLIAALLATLASGGAVLLPARGRFSA 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1750 AYLAQLIERTGITTLHFVPSMLQQFVQWADADCACD---SLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDV 1826
Cdd:PRK05852  257 HTFWDDIKAVGATWYTAVPTIHQILLERAATEPSGRkpaALRFIRSCSAPLTAETAQALQTEFAAPVVCAFGMTEATHQV 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1827 T----FWACQPDDHRSFV-PIGRPIAnTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpg 1901
Cdd:PRK05852  337 TttqiEGIGQTENPVVSTgLVGRSTG-AQIRIVGSDGLPLPAGAVGEVWLRGTTVVRGYLGDPTITAANFTDGWL----- 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1902 arlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPD 1981
Cdd:PRK05852  411 ----RTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPRESAPPT 486

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967 1982 PADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK05852  487 AEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAV 526
ACS-like cd17634
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ...
 449-958 4.19e-35

acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341289 [Multi-domain]  Cd Length: 587  Bit Score: 144.26  E-value: 4.19e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  449 ILPKSERQQVLVDFNATDADFPREMLIQQRFEA---QAEQTPEAIAVLFED------QHLTYRELNRRANQLAHHLIALG 519
Cdd:cd17634    26 ITPYQKVKNTSFAPGAPSIKWFEDATLNLAANAldrHLRENGDRTAIIYEGddtsqsRTISYRELHREVCRFAGTLLDLG 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  520 VQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS---------------AQVAQL 584
Cdd:cd17634   106 VKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLITADggvragrsvplkknvDDALNP 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  585 NSTLP-TVLL------------------DTPAAAACPDTNPVvqGLHAAHLAYVIYTSGSTGRPKGVMVAHRN-VINLAT 644
Cdd:cd17634   186 NVTSVeHVIVlkrtgsdidwqegrdlwwRDLIAKASPEHQPE--AMNAEDPLFILYTSGTTGKPKGVLHTTGGyLVYAAT 263

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  645 GLHTLLALdHPSRIALNASIVFDASVKNWI---QLLSGHTLVL---VPDALRADAhqLWRYFARHAVDLFDCTPVQLQWL 718
Cdd:cd17634   264 TMKYVFDY-GPGDIYWCTADVGWVTGHSYLlygPLACGATTLLyegVPNWPTPAR--MWQVVDKHGVNILYTAPTAIRAL 340

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  719 LDAGLGSDPAYQPA--QVLIG-GEAISPAVWS---RLQSLSDTRFINVYGPTECtvdATACVVDRTQPLPTIG----KPL 788
Cdd:cd17634   341 MAAGDDAIEGTDRSslRILGSvGEPINPEAYEwywKKIGKEKCPVVDTWWQTET---GGFMITPLPGAIELKAgsatRPV 417

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  789 ANTRLYILDAQDQPVPIGVTGELHIGGA--GVARGYLHRPDltaeRFIPDPFSAdpAARIYKTGDLARWLPDGNIDYLGR 866
Cdd:cd17634   418 FGVQPAVVDNEGHPQPGGTEGNLVITDPwpGQTRTLFGDHE----RFEQTYFST--FKGMYFSGDGARRDEDGYYWITGR 491

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  867 NDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHP---AALRQQLAASLADYMIPSA 943
Cdd:cd17634   492 SDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPelyAELRNWVRKEIGPLATPDV 571

                         570
                  ....*....|....*
gi 641744967  944 FVTLDALPLTPNGKL 958
Cdd:cd17634   572 VHWVDSLPKTRSGKI 586
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
 2608-3097 4.30e-35

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 142.65  E-value: 4.30e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGEAS--LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVP 2685
Cdd:cd05923     3 VFEMLRRAASRAPDACAIADPARGlrLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPAL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2686 LDPTYPVERLRYMLDDAKPVALISQSAHL---GIMNGSLPVILLDDgETRPFDNEPDTPLDARKQGlTPRHLAYVIYTSG 2762
Cdd:cd05923    83 INPRLKAAELAELIERGEMTAAVIAVDAQvmdAIFQSGVRVLALSD-LVGLGEPESAGPLIEDPPR-EPEQPAFVFYTSG 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2763 STGKPKGVMVEHANMVNFLCSMRKEPGI--AQEDVLLGVTSLSFDISILEIFL-PLLNGARLILATQAQAADAQQlamLI 2839
Cdd:cd05923   161 TTGLPKGAVIPQRAAESRVLFMSTQAGLrhGRHNVVLGLMPLYHVIGFFAVLVaALALDGTYVVVEEFDPADALK---LI 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2840 ERHAVSFMQATPSTWRMLVELRDFAlPPGFKAL----CGGEALPENLATALLQKVTTLW-NLYGPTEttiwsTLNGLTTP 2914
Cdd:cd05923   238 EQERVTSLFATPTHLDALAAAAEFA-GLKLSSLrhvtFAGATMPDAVLERVNQHLPGEKvNIYGTTE-----AMNSLYMR 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2915 TPYIG---HPIANTQIYILDAQGRVV---PLGVAGEIHIAGAG--VVRGYLGRPDLTAERfITDpfsgapeaRMYKTGDL 2986
Cdd:cd05923   312 DARTGtemRPGFFSEVRIVRIGGSPDealANGEEGELIVAAAAdaAFTGYLNQPEATAKK-LQD--------GWYRTGDV 382

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2987 GRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLS 3066
Cdd:cd05923   383 GYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREGTLSADELDQFCRA 462

                         490       500       510
                  ....*....|....*....|....*....|.
gi 641744967 3067 EGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05923   463 SELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
PRK07638 PRK07638
acyl-CoA synthetase; Validated
 1536-2021 7.38e-35

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 141.84  E-value: 7.38e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1536 DQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDdRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAE 1615
Cdd:PRK07638    9 KHASLQPNKIAIKENDRVLTYKDWFESVCKVANWLNEKESKNK-TIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQD 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1616 RLAYMLDDASPVALLTQANQRALLTG-DVPRILLDTADFSHLSEDNPHVPGLDAHHLA-YVIYTSGSTGKPKGVMNSHRA 1693
Cdd:PRK07638   88 ELKERLAISNADMIVTERYKLNDLPDeEGRVIEIDEWKRMIEKYLPTYAPIENVQNAPfYMGFTSGSTGKPKAFLRAQQS 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1694 lcnrlvWMQN------TYRLTPDDRVLqkTPFSFDVSVWEF--FWPLLYGARLVMAR---PDGHKDAaylaqlIERTGIT 1762
Cdd:PRK07638  168 ------WLHSfdcnvhDFHMKREDSVL--IAGTLVHSLFLYgaISTLYVGQTVHLMRkfiPNQVLDK------LETENIS 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1763 TLHFVPSMLQQFVQwadADCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIdVTFWACQpDDHRSFVP 1841
Cdd:PRK07638  234 VMYTVPTMLESLYK---ENRVIENKMKIISSGAKWEAEAKEKIKNIFpYAKLYEFYGASELSF-VTALVDE-ESERRPNS 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1842 IGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNrpdltaerfipdpfinqpGARLYKTGDLARWLP------ 1915
Cdd:PRK07638  309 VGRPFHNVQVRICNEAGEEVQKGEIGTVYVKSPQFFMGYII------------------GGVLARELNADGWMTvrdvgy 370

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1916 ---DGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPgvtpDPADLRQQLGQH 1992
Cdd:PRK07638  371 edeEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAIIKGSA----TKQQLKSFCLQR 446

                         490       500
                  ....*....|....*....|....*....
gi 641744967 1993 LAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK07638  447 LSSFKIPKEWHFVDEIPYTNSGKIARMEA 475
PRK09274 PRK09274
peptide synthase; Provisional
 1536-2021 9.02e-35

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 142.73  E-value: 9.02e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1536 DQAARTPDTTAVLFED----------QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAY 1605
Cdd:PRK09274   14 RAAQERPDQLAVAVPGgrgadgklayDELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKAGAVP 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1606 VPLDPGYPAERLAYMLDDASPVALLTQ--------------ANQRALLTGDvPRILLDTADFSHLSEDNPHVPG----LD 1667
Cdd:PRK09274   94 VLVDPGMGIKNLKQCLAEAQPDAFIGIpkahlarrlfgwgkPSVRRLVTVG-GRLLWGGTTLATLLRDGAAAPFpmadLA 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1668 AHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTP-FSfdvsvweFFWPLLyGARLVM-----A 1741
Cdd:PRK09274  173 PDDMAAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIDLPTFPlFA-------LFGPAL-GMTSVIpdmdpT 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1742 RPdGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADA-DCACDSLRRVICSGEALPAELQQRFFARFN--AQLHNLYG 1818
Cdd:PRK09274  245 RP-ATVDPAKLFAAIERYGVTNLFGSPALLERLGRYGEAnGIKLPSLRRVISAGAPVPIAVIERFRAMLPpdAEILTPYG 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1819 PTEA---------AIDVTFWAcQPDDHRSfVPIGRPIANTQLYIL-----------DTLgqPVPLGVAGELHIGGVGVAR 1878
Cdd:PRK09274  324 ATEAlpissiesrEILFATRA-ATDNGAG-ICVGRPVDGVEVRIIaisdapipewdDAL--RLATGEIGEIVVAGPMVTR 399

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1879 GYLNRPDLTAERFIPDPfinQPGARlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVA 1958
Cdd:PRK09274  400 SYYNRPEATRLAKIPDG---QGDVW-HRMGDLGYLDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALVG 475

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1959 REdSPGDTRLVayLC--PQPGVTPDPADLRQQLGQHLAEY-MVPG--AFVTLDAFPLTP--NGKLDRKAL 2021
Cdd:PRK09274  476 VG-VPGAQRPV--LCveLEPGVACSKSALYQELRALAAAHpHTAGieRFLIHPSFPVDIrhNAKIFREKL 542
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
2597-3053 9.26e-35

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 143.70  E-value: 9.26e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2597 ATDADIPRHALIHELFEAQVACTPDAIAVVF----GEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVG 2672
Cdd:COG1022     2 SEFSDVPPADTLPDLLRRRAARFPDRVALREkedgIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIA 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2673 LLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALI-SQSAHLGIMN---GSLP----VILLDDGETRPFDN-------- 2736
Cdd:COG1022    82 DLAILAAGAVTVPIYPTSSAEEVAYILNDSGAKVLFvEDQEQLDKLLevrDELPslrhIVVLDPRGLRDDPRllsldell 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2737 ------EPDTPLDARKQGLTPRHLAYVIYTSGSTGKPKGVMVEHANMV-NFLCSMRKEPGIAQEDVLLgvtslsfdisil 2809
Cdd:COG1022   162 algrevADPAELEARRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLsNARALLERLPLGPGDRTLS------------ 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2810 eiFLPLLN-GARLIlatqaqaadaqQLAMLIERHAVSF-------------------------------------MQATP 2851
Cdd:COG1022   230 --FLPLAHvFERTV-----------SYYALAAGATVAFaespdtlaedlrevkptfmlavprvwekvyagiqakaEEAGG 296

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2852 STWRML----------VELRDFALPPGFK--------------------------ALCGGEALPENLAT-------ALLQ 2888
Cdd:COG1022   297 LKRKLFrwalavgrryARARLAGKSPSLLlrlkhaladklvfsklrealggrlrfAVSGGAALGPELARffralgiPVLE 376

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2889 kvttlwnLYGPTETTIWSTLNGLTTPTP-YIGHPIANTQIYIldaqgrvvplGVAGEIHIAGAGVVRGYLGRPDLTAERF 2967
Cdd:COG1022   377 -------GYGLTETSPVITVNRPGDNRIgTVGPPLPGVEVKI----------AEDGEILVRGPNVMKGYYKNPEATAEAF 439

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2968 ITDPFsgapearmYKTGDLGRWLPDGTLEYLGR--------NdfqvkvrGFRIELGEIETRLARCHGVHDAVVIaredsp 3039
Cdd:COG1022   440 DADGW--------LHTGDIGELDEDGFLRITGRkkdlivtsG-------GKNVAPQPIENALKASPLIEQAVVV------ 498

                         570
                  ....*....|....*.
gi 641744967 3040 GDKR--LVAylLAQPD 3053
Cdd:COG1022   499 GDGRpfLAA--LIVPD 512
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1676-2018 1.00e-34

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 138.18  E-value: 1.00e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1676 YTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPF--SFDvSVWEFFWPLLYGARLVMARPDGHKDAAYLA 1753
Cdd:cd05917     9 FTSGTTGSPKGATLTHHNIVNNGYFIGERLGLTEQDRLCIPVPLfhCFG-SVLGVLACLTHGATMVFPSPSFDPLAVLEA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1754 qlIERTGITTLHFVPSMlqqFV----QWADADCACDSLRRVICSGEALPAELQQRFFARFN-AQLHNLYGPTEAAiDVTF 1828
Cdd:cd05917    88 --IEKEKCTALHGVPTM---FIaeleHPDFDKFDLSSLRTGIMAGAPCPPELMKRVIEVMNmKDVTIAYGMTETS-PVST 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1829 wACQPDD--HRSFVPIGRPIANTQLYILDTLGQPVP-LGVAGELHIGGVGVARGYLNRPDLTAERFIPDpfinqpgaRLY 1905
Cdd:cd05917   162 -QTRTDDsiEKRVNTVGRIMPHTEAKIVDPEGGIVPpVGVPGELCIRGYSVMKGYWNDPEKTAEAIDGD--------GWL 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1906 KTGDLARWLPDGSLEYLGR-NDFQVKlRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPAD 1984
Cdd:cd05917   233 HTGDLAVMDEDGYCRIVGRiKDMIIR-GGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEGAELTEED 311

                         330       340       350
                  ....*....|....*....|....*....|....
gi 641744967 1985 LRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDR 2018
Cdd:cd05917   312 IKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQK 345
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
 475-963 1.06e-34

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 142.13  E-value: 1.06e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  475 IQQRFEAQAEQTPEAIAVLFED-----QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAA 549
Cdd:PRK08008    9 LRQMWDDLADVYGHKTALIFESsggvvRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAI 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  550 YVPMDPAYPAERLAYILDDAAPVALLTQSA------QVAQLNSTLPTVLLDTPAAAacPDTNPVV-----QGLHAAHLAY 618
Cdd:PRK08008   89 MVPINARLLREESAWILQNSQASLLVTSAQfypmyrQIQQEDATPLRHICLTRVAL--PADDGVSsftqlKAQQPATLCY 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  619 -----------VIYTSGSTGRPKGVMVAHRNVinLATGLHTLLaldhpsRIALNASIVF---------DASVKNWIQLLS 678
Cdd:PRK08008  167 applstddtaeILFTSGTTSRPKGVVITHYNL--RFAGYYSAW------QCALRDDDVYltvmpafhiDCQCTAAMAAFS 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  679 -GHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLdaglgsdpaYQPaqvliggeaisPAVWSRLQSLSD--- 754
Cdd:PRK08008  239 aGATFVLLE---KYSARAFWGQVCKYRATITECIPMMIRTLM---------VQP-----------PSANDRQHCLREvmf 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  755 -------------TRF----INVYGPTECTVDAtacVVDR---TQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIG 814
Cdd:PRK08008  296 ylnlsdqekdafeERFgvrlLTSYGMTETIVGI---IGDRpgdKRRWPSIGRPGFCYEAEIRDDHNRPLPAGEIGEICIK 372

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  815 G-AG--VARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCP 891
Cdd:PRK08008  373 GvPGktIFKEYYLDPKATAKVLEADGW--------LHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHP 444

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967  892 GVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK08008  445 KIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
PRK08315 PRK08315
AMP-binding domain protein; Validated
 1530-2015 1.14e-34

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 142.64  E-value: 1.14e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHL--TYDALNRRANQLAHHLIDLGVKPDDRIAI----CVErsldMVIGLLAILKAGA 1603
Cdd:PRK08315   18 IGQLLDRTAARYPDREALVYRDQGLrwTYREFNEEVDALAKGLLALGIEKGDRVGIwapnVPE----WVLTQFATAKIGA 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1604 AYVPLDPGYPAERLAYMLDDASPVALLT-----QANQRALLTGDVPRI------LLDTADFSHLSE----DNPHVPGL-- 1666
Cdd:PRK08315   94 ILVTINPAYRLSELEYALNQSGCKALIAadgfkDSDYVAMLYELAPELatcepgQLQSARLPELRRviflGDEKHPGMln 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1667 ---------DAHHLAY-----------VI---YTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPF---- 1719
Cdd:PRK08315  174 fdellalgrAVDDAELaarqatldpddPIniqYTSGTTGFPKGATLTHRNILNNGYFIGEAMKLTEEDRLCIPVPLyhcf 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1720 -------------SFDVSVWEFFWPLLygarlVMarpdghkdaaylaQLIERTGITTLHFVPSMlqqFV------QWADA 1780
Cdd:PRK08315  254 gmvlgnlacvthgATMVYPGEGFDPLA-----TL-------------AAVEEERCTALYGVPTM---FIaeldhpDFARF 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1781 DCAcdSLRRVICSGEALPAELQQRFFARFN-AQLHNLYGPTEA-------AIDvtfwacQPDDHR-SFVpiGRPIANTQL 1851
Cdd:PRK08315  313 DLS--SLRTGIMAGSPCPIEVMKRVIDKMHmSEVTIAYGMTETspvstqtRTD------DPLEKRvTTV--GRALPHLEV 382

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1852 YILD-TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINqpgarlykTGDLARWLPDGSLEYLGRndfqVK 1930
Cdd:PRK08315  383 KIVDpETGETVPRGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGWMH--------TGDLAVMDEEGYVNIVGR----IK 450

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1931 ---LRGfrielGE------IEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGA 2001
Cdd:PRK08315  451 dmiIRG-----GEniypreIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEEDVRDFCRGKIAHYKIPRY 525

                         570
                  ....*....|....
gi 641744967 2002 FVTLDAFPLTPNGK 2015
Cdd:PRK08315  526 IRFVDEFPMTVTGK 539
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
 2631-3097 1.19e-34

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 139.40  E-value: 1.19e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPValisq 2710
Cdd:cd05912     1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVK----- 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2711 sahlgimngslpvilLDDgetrpfdnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGI 2790
Cdd:cd05912    76 ---------------LDD-------------------------IATIMYTSGTTGKPKGVQQTFGNHWWSAIGSALNLGL 115

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2791 AQEDVLLGVTSLsFDISILEIFL-PLLNGARLILATQAQAADAQQlamLIERHAVSFMQATPSTWRMLVELRDFALPPGF 2869
Cdd:cd05912   116 TEDDNWLCALPL-FHISGLSILMrSVIYGMTVYLVDKFDAEQVLH---LINSGKVTIISVVPTMLQRLLEILGEGYPNNL 191

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2870 KA-LCGGEALPENLATALLQKVTTLWNLYGPTETTIWS-TLNGLTTPTPY--IGHPIANTQIYILDAQGrvvPLGVAGEI 2945
Cdd:cd05912   192 RCiLLGGGPAPKPLLEQCKEKGIPVYQSYGMTETCSQIvTLSPEDALNKIgsAGKPLFPVELKIEDDGQ---PPYEVGEI 268

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2946 HIAGAGVVRGYLGRPDLTAERFITDPFsgapearmyKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCH 3025
Cdd:cd05912   269 LLKGPNVTKGYLNRPDATEESFENGWF---------KTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHP 339

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967 3026 GVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEpaDLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05912   340 AIKEAGVVGIPDDKWGQVPVAFVVSERPISEE--ELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHEL 409
C_NRPS-like cd19066
Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of ...
 2162-2566 2.05e-34

Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long, with various activities such as antibiotic, antifungal, antitumor and immunosuppression. There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380453 [Multi-domain]  Cd Length: 427  Bit Score: 139.08  E-value: 2.05e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2162 YPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCwQDLSQPVQVVwrqailpinh 2241
Cdd:cd19066     2 IPLSPMQRGMWFLKKLATDPSAFNVAIEMFLTGSLDLARLKQALDAVMERHDVLRTRFC-EEAGRYEQVV---------- 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2242 FEPTSPEDV-LAQLQAHTEPRTRRIDLSQAPLFRA-DIAHDPL---------QNEWLLALSFHHLISDHMTLALIVGEIR 2310
Cdd:cd19066    71 LDKTVRFRIeIIDLRNLADPEARLLELIDQIQQTIyDLERGPLvrvalfrlaDERDVLVVAIHHIIVDGGSFQILFEDIS 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2311 LL---LQHQADALPTPL-PYRNFIA----QTLSVPNSAHEAYFRDKLADVDEPtAPFGLLNVQG--SGGDIHEARLVLDA 2380
Cdd:cd19066   151 SVydaAERQKPTLPPPVgSYADYAAwlekQLESEAAQADLAYWTSYLHGLPPP-LPLPKAKRPSqvASYEVLTLEFFLRS 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2381 TLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLagAEGADRIMGMFINTLPLRISLA-DRGAAEV 2459
Cdd:cd19066   230 EETKRLREVARESGTTPTQLLLAAFALALKRLTASIDVVIGLTFLNRP--DEAVEDTIGLFLNLLPLRIDTSpDATFPEL 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2460 VERTSHDLMTLLEHEQAPLALAQRCSGVAPPM---PLFSTLLNYRHTQA--SSTDNTLSDIRVLTSEERTNYPLTLAVDD 2534
Cdd:cd19066   308 LKRTKEQSREAIEHQRVPFIELVRHLGVVPEApkhPLFEPVFTFKNNQQqlGKTGGFIFTTPVYTSSEGTVFDLDLEASE 387

                         410       420       430
                  ....*....|....*....|....*....|....*..
gi 641744967 2535 RGEG-----FSLVAQTLEDIDPHRLLNYLMTAISSLV 2566
Cdd:cd19066   388 DPDGdlllrLEYSRGVYDERTIDRFAERYMTALRQLI 424
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
1540-2021 3.33e-34

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 140.90  E-value: 3.33e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1540 RTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAyvPLDPGYPAERL-- 1617
Cdd:PRK10946   35 AASDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--PVNALFSHQRSel 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 -AYMlDDASPvALLTQANQRALLTGDV-----------PRILL---DTADFS---HLSEDNPHVPGLD--AHHLAYVIYT 1677
Cdd:PRK10946  113 nAYA-SQIEP-ALLIADRQHALFSDDDflntlvaehssLRVVLllnDDGEHSlddAINHPAEDFTATPspADEVAFFQLS 190

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1678 SGSTGKPKGVMNSHralcnrlvwmqNTY-----------RLTPDDRVLQKTP--FSFDVSVWEFFWPLLYGARLVMArPD 1744
Cdd:PRK10946  191 GGSTGTPKLIPRTH-----------NDYyysvrrsveicGFTPQTRYLCALPaaHNYPMSSPGALGVFLAGGTVVLA-PD 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1745 ghKDAAYLAQLIERTGITTLHFVP---SMLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTE 1821
Cdd:PRK10946  259 --PSATLCFPLIEKHQVNVTALVPpavSLWLQAIAEGGSRAQLASLKLLQVGGARLSETLARRIPAELGCQLQQVFGMAE 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1822 AAIDVTfwACQPDDHRSFVPIGRPIA-NTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqp 1900
Cdd:PRK10946  337 GLVNYT--RLDDSDERIFTTQGRPMSpDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGF---- 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1901 garlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLcpqpgVTP 1980
Cdd:PRK10946  411 ----YCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFL-----VVK 481

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*
gi 641744967 1981 DP---ADLRQQL-GQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK10946  482 EPlkaVQLRRFLrEQGIAEFKLPDRVECVDSLPLTAVGKVDKKQL 526
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
 1552-1957 3.43e-34

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 139.42  E-value: 3.43e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1552 QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLT 1631
Cdd:cd17640     4 KRITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVV 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1632 QAnqralltgdvprilldtadfshlSEDNphvpgldahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDD 1711
Cdd:cd17640    84 EN-----------------------DSDD----------LATIIYTSGTTGNPKGVMLTHANLLHQIRSLSDIVPPQPGD 130

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1712 RVLQKTP--FSFDVSVWEFFwpLLYGARLVMARPDGHKD--AAYLAQLIE---------RTGITTLHFVPSMLQQFVqwA 1778
Cdd:cd17640   131 RFLSILPiwHSYERSAEYFI--FACGCSQAYTSIRTLKDdlKRVKPHYIVsvprlweslYSGIQKQVSKSSPIKQFL--F 206

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1779 DADCACDSLRRVICSGEALPAELQqRFFARFNAQLHNLYGPTEAAIDVTfwaCQPDDHRSFVPIGRPIANTQLYILDTLG 1858
Cdd:cd17640   207 LFFLSGGIFKFGISGGGALPPHVD-TFFEAIGIEVLNGYGLTETSPVVS---ARRLKCNVRGSVGRPLPGTEIKIVDPEG 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1859 Q-PVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINqpgarlykTGDLARWLPDGSLEYLGR-NDFQVKLRGFRI 1936
Cdd:cd17640   283 NvVLPPGEKGIVWVRGPQVMKGYYKNPEATSKVLDSDGWFN--------TGDLGWLTCGGELVLTGRaKDTIVLSNGENV 354

                         410       420
                  ....*....|....*....|.
gi 641744967 1937 ELGEIEARLMQCPGVQEAVVV 1957
Cdd:cd17640   355 EPQPIEEALMRSPFIEQIMVV 375
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
480-963 3.63e-34

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 140.79  E-value: 3.63e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  480 EAQAEQTPEAIAVLFEDQH--LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAY 557
Cdd:PRK05852   23 EVAATRLPEAPALVVTADRiaISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPAL 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  558 P-AERLAYILDDAAPVALLTQSAQVAQLNSTLP-----------------TVLLDTPAAAACPDTNPVVQGLHAAHlAYV 619
Cdd:PRK05852  103 PiAEQRVRSQAAGARVVLIDADGPHDRAEPTTRwwpltvnvggdsgpsggTLSVHLDAATEPTPATSTPEGLRPDD-AMI 181

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  620 IYTSGSTGRPKGVMVAHRNVinlATGLHTLLAL----DHPSRIALNASIVFDASVKNWIQLLSGHTLVLVPDALRADAHQ 695
Cdd:PRK05852  182 MFTGGTTGLPKMVPWTHANI---ASSVRAIITGyrlsPRDATVAVMPLYHGHGLIAALLATLASGGAVLLPARGRFSAHT 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  696 LWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRLQSLSDT---RFINVYGPTECTVDATA 772
Cdd:PRK05852  259 FWDDIKAVGATWYTAVPTIHQILLERAATEPSGRKPAALRFIRSCSAPLTAETAQALQTEfaaPVVCAFGMTEATHQVTT 338

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  773 CVVD---RTQ-PLPTIGKPLANT--RLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaari 846
Cdd:PRK05852  339 TQIEgigQTEnPVVSTGLVGRSTgaQIRIVGSDGLPLPAGAVGEVWLRGTTVVRGYLGDPTITAANFTDGWL-------- 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  847 yKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAA 926
Cdd:PRK05852  411 -RTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPRESAPPTAEE 489

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 641744967  927 LRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK05852  490 LVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAV 526
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
 472-941 3.83e-34

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 141.55  E-value: 3.83e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  472 EMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYV 551
Cdd:PRK08279   36 KRSLGDVFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVA 115

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  552 PMDPAYPAERLAYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTP--------------------AAAACPDTNPVV-QG 610
Cdd:PRK08279  116 LLNTQQRGAVLAHSLNLVDAKHLIVGEELVEAFEEARADLARPPRlwvaggdtlddpegyedlaaAAAGAPTTNPASrSG 195

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  611 LHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIAL-------NASIVFDASVknwiqLLSGHTLV 683
Cdd:PRK08279  196 VTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMGGFGGLLRLTPDDVLYCclplyhnTGGTVAWSSV-----LAAGATLA 270

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  684 LVPdalRADAHQLWRYFARHavdlfDCTPVQ-----LQWLLDaglgsdpayQPAQ--------VLIGGEAISPAVWSRLQ 750
Cdd:PRK08279  271 LRR---KFSASRFWDDVRRY-----RATAFQyigelCRYLLN---------QPPKptdrdhrlRLMIGNGLRPDIWDEFQ 333

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  751 slsdTRF-----INVYGPTECTV--------DATACVVdrtqplPTIGK-PLA------NTRLYILDAQD--QPVPIGVT 808
Cdd:PRK08279  334 ----QRFgipriLEFYAASEGNVgfinvfnfDGTVGRV------PLWLAhPYAivkydvDTGEPVRDADGrcIKVKPGEV 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  809 GEL--HIGGAGVARGYLhRPDLTAERFIPDPFSadPAARIYKTGDLARwlPDGN-----IDYLGrnD-FQIKvrGFRIEA 880
Cdd:PRK08279  404 GLLigRITDRGPFDGYT-DPEASEKKILRDVFK--KGDAWFNTGDLMR--DDGFghaqfVDRLG--DtFRWK--GENVAT 474

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967  881 GEIESRLLRCPGVQDAVVIAREdSPG-DTRL-VAYLCARPDAELHPAALRQQLAASLADYMIP 941
Cdd:PRK08279  475 TEVENALSGFPGVEEAVVYGVE-VPGtDGRAgMAAIVLADGAEFDLAALAAHLYERLPAYAVP 536
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
 1531-2021 4.30e-34

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 141.54  E-value: 4.30e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQAARTPDTTAVLFE------DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAA 1604
Cdd:cd05966    56 YNCLDRHLKERGDKVAIIWEgdepdqSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAV 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1605 YVPLDPGYPAERLAYMLDDASPVALLTqANQraLLTGDVPRILLDTAD--------------FSHLSEDNPHVPG----- 1665
Cdd:cd05966   136 HSVVFAGFSAESLADRINDAQCKLVIT-ADG--GYRGGKVIPLKEIVDealekcpsvekvlvVKRTGGEVPMTEGrdlww 212

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1666 ---------------LDAHHLAYVIYTSGSTGKPKGVMNSHRALcnrLVW----MQNTYRLTPDDRvlqktpfsfdvsvw 1726
Cdd:cd05966   213 hdlmakqspecepewMDSEDPLFILYTSGSTGKPKGVVHTTGGY---LLYaattFKYVFDYHPDDI-------------- 275

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1727 efFW-----------------PLLYGARLVM--ARPDgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADC-ACD- 1785
Cdd:cd05966   276 --YWctadigwitghsyivygPLANGATTVMfeGTPT-YPDPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVkKHDl 352

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1786 -SLRrVICS-GEALPAELQQRFfarfnaqlHNLYGPTEAAIDVTFW-------------ACQPDDHRSfvpIGRPIANTQ 1850
Cdd:cd05966   353 sSLR-VLGSvGEPINPEAWMWY--------YEVIGKERCPIVDTWWqtetggimitplpGATPLKPGS---ATRPFFGIE 420

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1851 LYILDTLGQPVPLGVAGELHIGGV--GVARGYLNRPdltaERFIPDPFINQPGarLYKTGDLARWLPDGSLEYLGRNDFQ 1928
Cdd:cd05966   421 PAILDEEGNEVEGEVEGYLVIKRPwpGMARTIYGDH----ERYEDTYFSKFPG--YYFTGDGARRDEDGYYWITGRVDDV 494

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1929 VKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPaDLRQQLGQHLAEymVPGAFVTLDAF 2008
Cdd:cd05966   495 INVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSD-ELRKELRKHVRK--EIGPIATPDKI 571

                         570
                  ....*....|....*....
gi 641744967 2009 ------PLTPNGKLDRKAL 2021
Cdd:cd05966   572 qfvpglPKTRSGKIMRRIL 590
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
 1533-2021 8.45e-34

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 139.51  E-value: 8.45e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1533 LVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAI-CVERS--LDMVIGLLAIlkaGAAYVPLD 1609
Cdd:PRK06155   26 MLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALmCGNRIefLDVFLGCAWL---GAIAVPIN 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1610 PGYPAERLAYMLDDASPVALLTQANQ----RALLTGDVPR---ILLDTADFSHLSE--DNPHVPGLDAH---------HL 1671
Cdd:PRK06155  103 TALRGPQLEHILRNSGARLLVVEAALlaalEAADPGDLPLpavWLLDAPASVSVPAgwSTAPLPPLDAPapaaavqpgDT 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1672 AYVIYTSGSTGKPKGVMNSHRALcnrLVWMQNTYR---LTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMAR---PDG 1745
Cdd:PRK06155  183 AAILYTSGTTGPSKGVCCPHAQF---YWWGRNSAEdleIGADDVLYTTLPLFHTNALNAFFQALLAGATYVLEPrfsASG 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1746 HKDAAylaqliERTGITTLHFVPSMLQQFV-QWADADCACDSLRRVIcsGEALPAELQQRFFARFNAQLHNLYGPTEAai 1824
Cdd:PRK06155  260 FWPAV------RRHGATVTYLLGAMVSILLsQPARESDRAHRVRVAL--GPGVPAALHAAFRERFGVDLLDGYGSTET-- 329

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1825 DVTFWACQPDDHRSFvpIGRPIANTQLYILDTLGQPVPLGVAGEL---HIGGVGVARGYLNRPDLTAERFIPDPFinqpg 1901
Cdd:PRK06155  330 NFVIAVTHGSQRPGS--MGRLAPGFEARVVDEHDQELPDGEPGELllrADEPFAFATGYFGMPEKTVEAWRNLWF----- 402

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1902 arlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPD 1981
Cdd:PRK06155  403 ----HTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLRDGTALE 478

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967 1982 PADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK06155  479 PVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVL 518
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
 1537-2021 1.17e-33

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 139.34  E-value: 1.17e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1537 QAARTPDTTAVLFED-----QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:cd05906    18 RAAERGPTKGITYIDadgseEFQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLTVP 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 ----YPAERLAY------MLDdaSPVALLTQANQ---RALLTGD-VPRILLDTADFSHLSEDNPHVPGLDAHHLAYVIYT 1677
Cdd:cd05906    98 ptydEPNARLRKlrhiwqLLG--SPVVLTDAELVaefAGLETLSgLPGIRVLSIEELLDTAADHDLPQSRPDDLALLMLT 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1678 SGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEF-FWPLLYGARLVMARPDghkdaAYLAQ-- 1754
Cdd:cd05906   176 SGSTGFPKAVPLTHRNILARSAGKIQHNGLTPQDVFLNWVPLDHVGGLVELhLRAVYLGCQQVHVPTE-----EILADpl 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1755 ----LIERTGITTL---HFVPSMLQQFVQWADADCA-CDSLRRVICSGEALPAELQQRF---FARFNAQ---LHNLYGPT 1820
Cdd:cd05906   251 rwldLIDRYRVTITwapNFAFALLNDLLEEIEDGTWdLSSLRYLVNAGEAVVAKTIRRLlrlLEPYGLPpdaIRPAFGMT 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1821 EAAIDVTFWA-CQPDDHR---SFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPF 1896
Cdd:cd05906   331 ETCSGVIYSRsFPTYDHSqalEFVSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPEANAEAFTEDGW 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1897 inqpgarlYKTGDLArWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVA---REDSPGDTRLVAYLC 1973
Cdd:cd05906   411 --------FRTGDLG-FLDNGNLTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPSFTAAfavRDPGAETEELAIFFV 481

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1974 PQPGVTPDPADLRQQLGQHLA-------EYMVPgafVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05906   482 PEYDLQDALSETLRAIRSVVSrevgvspAYLIP---LPKEEIPKTSLGKIQRSKL 533
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
 480-968 1.26e-33

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 139.40  E-value: 1.26e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  480 EAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPA 559
Cdd:PRK06710   31 EQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTE 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  560 ERLAYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNPVVQGL-------------------HAAHL---- 616
Cdd:PRK06710  111 RELEYQLHDSGAKVILCLDLVFPRVTNVQSATKIEHVIVTRIADFLPFPKNLlypfvqkkqsnlvvkvsesETIHLwnsv 190

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  617 ------------------AYVIYTSGSTGRPKGVMVAHRNVI-NLATGLHTLLALDHPSRIALNASIVFD----ASVKNw 673
Cdd:PRK06710  191 ekevntgvevpcdpendlALLQYTGGTTGFPKGVMLTHKNLVsNTLMGVQWLYNCKEGEEVVLGVLPFFHvygmTAVMN- 269

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  674 IQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPA-VWSRLQSL 752
Cdd:PRK06710  270 LSIMQGYKMVLIP---KFDMKMVFEAIKKHKVTLFPGAPTIYIALLNSPLLKEYDISSIRACISGSAPLPVeVQEKFETV 346

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  753 SDTRFINVYGPTECT-VDATACVVDRTQPlPTIGKPLANTRLYILDAQD-QPVPIGVTGELHIGGAGVARGYLHRPDLTA 830
Cdd:PRK06710  347 TGGKLVEGYGLTESSpVTHSNFLWEKRVP-GSIGVPWPDTEAMIMSLETgEALPPGEIGEIVVKGPQIMKGYWNKPEETA 425

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  831 ErfipdpfsadpaarIYKTGdlarWLPDGNIDYLGRNDF---------QIKVRGFRIEAGEIESRLLRCPGVQDAVVIAR 901
Cdd:PRK06710  426 A--------------VLQDG----WLHTGDVGYMDEDGFfyvkdrkkdMIVASGFNVYPREVEEVLYEHEKVQEVVTIGV 487

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967  902 EDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQ 968
Cdd:PRK06710  488 PDPYRGETVKAFVVLKEGTECSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIEEEK 554
PRK07470 PRK07470
acyl-CoA synthetase; Validated
 1532-2021 1.30e-33

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 139.02  E-value: 1.30e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1532 QLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:PRK07470   11 HFLRQAARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFR 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 YPAERLAYMLDDASPVALLTQAN-------QRALLTGDVPRILLDTADFSH----LSEDNPHVPGLDAH----HLAYVIY 1676
Cdd:PRK07470   91 QTPDEVAYLAEASGARAMICHADfpehaaaVRAASPDLTHVVAIGGARAGLdyeaLVARHLGARVANAAvdhdDPCWFFF 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1677 TSGSTGKPKGVMNSHRALC----NRLV-WMQNTyrlTPDDRVLQKTPFSFDVSVWEffwpLLYGARLVMA--RPDGHKDA 1749
Cdd:PRK07470  171 TSGTTGRPKAAVLTHGQMAfvitNHLAdLMPGT---TEQDASLVVAPLSHGAGIHQ----LCQVARGAATvlLPSERFDP 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1750 AYLAQLIERTGITTLHFVPSMLQQFVQWADADcACD--SLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVT 1827
Cdd:PRK07470  244 AEVWALVERHRVTNLFTVPTILKMLVEHPAVD-RYDhsSLRYVIYAGAPMYRADQKRALAKLGKVLVQYFGLGEVTGNIT 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1828 -----FWACQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpga 1902
Cdd:PRK07470  323 vlppaLHDAEDGPDARIGTCGFERTGMEVQIQDDEGRELPPGETGEICVIGPAVFAGYYNNPEANAKAFRDGWF------ 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1903 rlyKTGDLARWLPDGSLEYLGR-NDFQVKlRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPD 1981
Cdd:PRK07470  397 ---RTGDLGHLDARGFLYITGRaSDMYIS-GGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVD 472

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967 1982 PADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK07470  473 EAELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMV 512
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
487-963 1.52e-33

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 138.97  E-value: 1.52e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAyvPMDPAYPAERL---A 563
Cdd:PRK10946   37 SDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--PVNALFSHQRSelnA 114

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  564 YIlDDAAPvALLTQSAQ---------VAQLNSTLPTV----------------LLDTPAAAACPDTNPvvqglhAAHLAY 618
Cdd:PRK10946  115 YA-SQIEP-ALLIADRQhalfsdddfLNTLVAEHSSLrvvlllnddgehslddAINHPAEDFTATPSP------ADEVAF 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  619 VIYTSGSTGRPKGVMVAH--------RNVINLATGLHT--LLALDHPSRIALN---ASIVFDAsvknwiqllsGHTLVLV 685
Cdd:PRK10946  187 FQLSGGSTGTPKLIPRTHndyyysvrRSVEICGFTPQTryLCALPAAHNYPMSspgALGVFLA----------GGTVVLA 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  686 PDAlraDAHQLWRYFARHAVDLFDCTP--VQLqWLLDAGL-GSDPAYQPAQVL-IGGEAISPAVWSRLQSLSDTRFINVY 761
Cdd:PRK10946  257 PDP---SATLCFPLIEKHQVNVTALVPpaVSL-WLQAIAEgGSRAQLASLKLLqVGGARLSETLARRIPAELGCQLQQVF 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  762 GPTECTVDATACVVDRTQPLPTIGKPLA-NTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsa 840
Cdd:PRK10946  333 GMAEGLVNYTRLDDSDERIFTTQGRPMSpDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGF-- 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  841 dpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARpdA 920
Cdd:PRK10946  411 ------YCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVK--E 482

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 641744967  921 ELHPAALRQQL-AASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK10946  483 PLKAVQLRRFLrEQGIAEFKLPDRVECVDSLPLTAVGKVDKKQL 526
MACS_like_2 cd05973
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 499-963 1.66e-33

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341277 [Multi-domain]  Cd Length: 437  Bit Score: 136.88  E-value: 1.66e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:cd05973     1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTDA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 AQVAQLnstlptvlldtpaaaacpDTNPVVQglhaahlayvIYTSGSTGRPKGVMVAHRNVinLATGLHTLLALDHPSri 658
Cdd:cd05973    81 ANRHKL------------------DSDPFVM----------MFTSGTTGLPKGVPVPLRAL--AAFGAYLRDAVDLRP-- 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  659 alnASIVFDASVKNWI---------QLLSGHTLVLVPDALRADAhqLWRYFARHAVDLFDCTPVQLQWLLDAGLGS--DP 727
Cdd:cd05973   129 ---EDSFWNAADPGWAyglyyaitgPLALGHPTILLEGGFSVES--TWRVIERLGVTNLAGSPTAYRLLMAAGAEVpaRP 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  728 AYQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTEC-TVDATACVVDRTQPLPTIGKPLANTRLYILD-AQDQPVPi 805
Cdd:cd05973   204 KGRLRRVSSAGEPLTPEVIRWFDAALGVPIHDHYGQTELgMVLANHHALEHPVHAGSAGRAMPGWRVAVLDdDGDELGP- 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  806 GVTGELHIGGAGVA----RGYLHRPDltaerfipdpfsADPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAG 881
Cdd:cd05973   283 GEPGRLAIDIANSPlmwfRGYQLPDT------------PAIDGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPF 350

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  882 EIESRLLRCPGVQDAVVIAREDsPGDTRLV-AYLCARPDAELHPA---ALRQQLAASLADYMIPSAFVTLDALPLTPNGK 957
Cdd:cd05973   351 DVESALIEHPAVAEAAVIGVPD-PERTEVVkAFVVLRGGHEGTPAladELQLHVKKRLSAHAYPRTIHFVDELPKTPSGK 429


                  ....*.
gi 641744967  958 LDRKAL 963
Cdd:cd05973   430 IQRFLL 435
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
 1529-2023 1.77e-33

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 139.93  E-value: 1.77e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1529 LIHQLVEDQAARTPDTTAVLFEDQH-----LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGA 1603
Cdd:cd05968    62 IVEQLLDKWLADTRTRPALRWEGEDgtsrtLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGG 141

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1604 AYVPLDPGYPAERLAYMLDDASPVALLTqanQRALLTGDVPRILLDTADFS--------------HLSEDNPHVPGLD-- 1667
Cdd:cd05968   142 IVVPIFSGFGKEAAATRLQDAEAKALIT---ADGFTRRGREVNLKEEADKAcaqcptvekvvvvrHLGNDFTPAKGRDls 218

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1668 --------AHHLA--------YVIYTSGSTGKPKGVMNSHRALCNRLVW-MQNTYRLTPDDRVLQKTPFSFDVSVWEFFW 1730
Cdd:cd05968   219 ydeeketaGDGAErtesedplMIIYTSGTTGKPKGTVHVHAGFPLKAAQdMYFQFDLKPGDLLTWFTDLGWMMGPWLIFG 298

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1731 PLLYGARLVM--ARPDgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLR--RVICS-GEALPAELQQRF 1805
Cdd:cd05968   299 GLILGATMVLydGAPD-HPKADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSslRVLGStGEPWNPEPWNWL 377

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1806 FARF---NAQLHNLYGPTEaaIDVTFWACQPddHRSFVPIG--RPIANTQLYILDTLGQPVPlGVAGELHIGG--VGVAR 1878
Cdd:cd05968   378 FETVgkgRNPIINYSGGTE--ISGGILGNVL--IKPIKPSSfnGPVPGMKADVLDESGKPAR-PEVGELVLLApwPGMTR 452

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1879 GYLNRPDltaeRFIPDPFINQPGARLYktGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVA 1958
Cdd:cd05968   453 GFWRDED----RYLETYWSRFDNVWVH--GDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIG 526

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 1959 REDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:cd05968   527 VPHPVKGEAIVCFVVLKPGVTPTEAlaeELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIRA 594
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
 2597-3095 2.09e-33

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 138.98  E-value: 2.09e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2597 ATDADIPRHALIHeLFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGI 2676
Cdd:PRK05605   24 PHDLDYGDTTLVD-LYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAV 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2677 LKAGGAYVPLDPTYPVERLRYMLDD--AK-------------------------PVALISQSAHLGIMNGSLPVILLDDG 2729
Cdd:PRK05605  103 LRLGAVVVEHNPLYTAHELEHPFEDhgARvaivwdkvaptverlrrttpletivSVNMIAAMPLLQRLALRLPIPALRKA 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2730 E---------TRPFDNEPDTPLDARKQGL-----TPRHLAYVIYTSGSTGKPKGVMVEHAN-MVNFLCSMRKEPGIA-QE 2793
Cdd:PRK05605  183 RaaltgpapgTVPWETLVDAAIGGDGSDVshprpTPDDVALILYTSGTTGKPKGAQLTHRNlFANAAQGKAWVPGLGdGP 262

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2794 DVLLGVTSL--SFDISILEIFLPLLnGARLILATQAQAADAQQlamLIERHAVSFMQATPSTWRMLVEL---RDFALPPG 2868
Cdd:PRK05605  263 ERVLAALPMfhAYGLTLCLTLAVSI-GGELVLLPAPDIDLILD---AMKKHPPTWLPGVPPLYEKIAEAaeeRGVDLSGV 338

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2869 FKALCGGEALPENLaTALLQKVT--TLWNLYGPTETTIWSTLNGLTTP--TPYIGHPIANTQIYILDAQ--GRVVPLGVA 2942
Cdd:PRK05605  339 RNAFSGAMALPVST-VELWEKLTggLLVEGYGLTETSPIIVGNPMSDDrrPGYVGVPFPDTEVRIVDPEdpDETMPDGEE 417

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2943 GEIHIAGAGVVRGYLGRPDLTAERFITDpfsgapearMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLA 3022
Cdd:PRK05605  418 GELLVRGPQVFKGYWNRPEETAKSFLDG---------WFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLR 488

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967 3023 RCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRK 3095
Cdd:PRK05605  489 EHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALDPEGLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRR 561
E_NRPS cd19534
Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 1067-1339 2.52e-33

Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Epimerization (E) domains of nonribosomal peptide synthetases (NRPS) flip the chirality of the end amino acid of a peptide being manufactured by the NRPS. E-domains are homologous to the Condensation (C) domains. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Specialized tailoring NRPS domains such as E-domains greatly increase the range of possible peptide products created by the NRPS machinery. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the E-domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380457 [Multi-domain]  Cd Length: 428  Bit Score: 135.84  E-value: 2.52e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1067 PLPLSFSQQrlWFLAQlDPAASQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLG-FS 1145
Cdd:cd19534     1 EVPLTPIQR--WFFEQ-NLAGRHHFNQSVLLRVPQGLDPDALRQALRALVEHHDALRMRFRREDGGWQQRIRGDVEElFR 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1146 LSSHDLRKLDEAARttrVAELAEqEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAA 1225
Cdd:cd19534    78 LEVVDLSSLAQAAA---IEALAA-EAQSSLDLEEGPLLAAALFDGTDGGDRLLLVIHHLVVDGVSWRILLEDLEAAYEQA 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1226 LEGSEANLPPLPvQYADYAVWQRQWLQGETLNDLRDYWRDQLQGAPAllEIPTDRPrpsvQRYAG-DQVPFHLDAGQLRR 1304
Cdd:cd19534   154 LAGEPIPLPSKT-SFQTWAELLAEYAQSPALLEELAYWRELPAADYW--GLPKDPE----QTYGDaRTVSFTLDEEETEA 226

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 641744967 1305 L-HALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVI 1339
Cdd:cd19534   227 LlQEANAAYRTEINDLLLAALALAFQDWTGRAPPAI 262
PRK13382 PRK13382
bile acid CoA ligase;
1538-2024 3.14e-33

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 137.97  E-value: 3.14e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:PRK13382   53 AQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPAL 132

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLTQANqralLTGDVPRILLDTADFSHLSE--DNPH---VPGLDAHHLA-----------YVIYTSGST 1681
Cdd:PRK13382  133 AEVVTREGVDTVIYDEE----FSATVDRALADCPQATRIVAwtDEDHdltVEVLIAAHAGqrpeptgrkgrVILLTSGTT 208

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1682 GKPKGVMNSHRAlcnrlvwmqntyrlTPDD--RVLQKTPFSFDVSV---------WEFFWPLLYGA---RLVMARpdgHK 1747
Cdd:PRK13382  209 GTPKGARRSGPG--------------GIGTlkAILDRTPWRAEEPTvivapmfhaWGFSQLVLAASlacTIVTRR---RF 271

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1748 DAAYLAQLIERTGITTLHFVPSMLQQFVQWAD---ADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAI 1824
Cdd:PRK13382  272 DPEATLDLIDRHRATGLAVVPVMFDRIMDLPAevrNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEAGM 351

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1825 DVTfwaCQPDDHRSFVPI-GRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYlnRPDLTAErfIPDPFINqpgar 1903
Cdd:PRK13382  352 IAT---ATPADLRAAPDTaGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGY--TSGSTKD--FHDGFMA----- 419

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1904 lykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA 1983
Cdd:PRK13382  420 ---SGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGASATPE 496

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 641744967 1984 DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAP 2024
Cdd:PRK13382  497 TLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQAR 537
PRK09029 PRK09029
O-succinylbenzoic acid--CoA ligase; Provisional
 1538-2021 5.62e-33

O-succinylbenzoic acid--CoA ligase; Provisional


Pssm-ID: 236363 [Multi-domain]  Cd Length: 458  Bit Score: 135.38  E-value: 5.62e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:PRK09029   13 AQVRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQPLL 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AymlddaspvALLTQANQRALLTGDVPRILLDTADFSHLSEDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNSHRA-LCN 1696
Cdd:PRK09029   93 E---------ELLPSLTLDFALVLEGENTFSALTSLHLQLVEGAHAVAWQPQRLATMTLTSGSTGLPKAAVHTAQAhLAS 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1697 R---LVWMQntyrLTPDDRVLQKTPFsFDVS----VWEffWpLLYGARLVMarPDGHKDAAYLAqliertGITTLHFVPS 1769
Cdd:PRK09029  164 AegvLSLMP----FTAQDSWLLSLPL-FHVSgqgiVWR--W-LYAGATLVV--RDKQPLEQALA------GCTHASLVPT 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1770 MLQQFVQWADADCacdSLRRVICSGEALPAELQQRffarfnAQLHNL-----YGPTEAAIDVTfwACQPDDHRSfvpIGR 1844
Cdd:PRK09029  228 QLWRLLDNRSEPL---SLKAVLLGGAAIPVELTEQ------AEQQGIrcwcgYGLTEMASTVC--AKRADGLAG---VGS 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1845 PIANTQLYIldtlgqpvplgVAGELHIGGVGVARGYLNRPDLTaerfipdPFINQPGarLYKTGDLARWLpDGSLEYLGR 1924
Cdd:PRK09029  294 PLPGREVKL-----------VDGEIWLRGASLALGYWRQGQLV-------PLVNDEG--WFATRDRGEWQ-NGELTILGR 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1925 NDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLcpQPGVTPDPADLRQQLGQHLAEYMVPGAFVT 2004
Cdd:PRK09029  353 LDNLFFSGGEGIQPEEIERVINQHPLVQQVFVVPVADAEFGQRPVAVV--ESDSEAAVVNLAEWLQDKLARFQQPVAYYL 430

                         490
                  ....*....|....*..
gi 641744967 2005 LDAFPLTPNGKLDRKAL 2021
Cdd:PRK09029  431 LPPELKNGGIKISRQAL 447
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
 483-958 6.61e-33

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 136.83  E-value: 6.61e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:PRK07786   27 ALMQPDAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEI 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLTQS------AQVAQLNSTLPTVL------------LDTPAAAACPDTNPVVQGLHAAhlAYVIYTSG 624
Cdd:PRK07786  107 AFLVSDCGAHVVVTEAalapvaTAVRDIVPLLSTVVvaggssddsvlgYEDLLAEAGPAHAPVDIPNDSP--ALIMYTSG 184

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  625 STGRPKGVMVAHRNVINLA-TGLHTlLALDHPSRIALNASIVFD-ASVKNWIQ-LLSGHTLVLVPdaLRA-DAHQLWRYF 700
Cdd:PRK07786  185 TTGRPKGAVLTHANLTGQAmTCLRT-NGADINSDVGFVGVPLFHiAGIGSMLPgLLLGAPTVIYP--LGAfDPGQLLDVL 261

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  701 ARHAVDLFDCTPVQLQWLLDAGlGSDPAYQPAQVLIGGEAisPAVWSRLQSLS----DTRFINVYGPTEctVDATACVV- 775
Cdd:PRK07786  262 EAEKVTGIFLVPAQWQAVCAEQ-QARPRDLALRVLSWGAA--PASDTLLRQMAatfpEAQILAAFGQTE--MSPVTCMLl 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  776 --DRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLA 853
Cdd:PRK07786  337 geDAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAF---------AGGWFHSGDLV 407

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  854 RWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPD-AELHPAALRQQLA 932
Cdd:PRK07786  408 RQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDdAALTLEDLAEFLT 487

                         490       500
                  ....*....|....*....|....*.
gi 641744967  933 ASLADYMIPSAFVTLDALPLTPNGKL 958
Cdd:PRK07786  488 DRLARYKHPKALEIVDALPRNPAGKV 513
C_PKS-NRPS cd19532
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 2163-2542 7.71e-33

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHxxxD motif, a few such as Monascus pilosus lovastatin nonaketide synthase MokA have a non-canonical HRxxxD motif in the C-domain and are unable to catalyze amide-bond formation. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380455 [Multi-domain]  Cd Length: 421  Bit Score: 134.51  E-value: 7.71e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2163 PLAPLQEGILF-HHLLQEQgDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQ-DLSQPVQVVWRQAILPIN 2240
Cdd:cd19532     3 PMSFGQSRFWFlQQYLEDP-TTFNVTFSYRLTGPLDVARLERAVRAVGQRHEALRTCFFTDpEDGEPMQGVLASSPLRLE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2241 HFEPTSPEDVLAQLQAHtepRTRRIDLSQAPLFRA-DIAHDPlqNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADa 2319
Cdd:cd19532    82 HVQISDEAEVEEEFERL---KNHVYDLESGETMRIvLLSLSP--TEHYLIFGYHHIAMDGVSFQIFLRDLERAYNGQPL- 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2320 LPTPLPYRNF-IAQTLSVPNSAHE---AYFRDKLADVDEPTAPFGLLNVQG----SGGDIHEARLVLDATLASAIRQQAR 2391
Cdd:cd19532   156 LPPPLQYLDFaARQRQDYESGALDedlAYWKSEFSTLPEPLPLLPFAKVKSrpplTRYDTHTAERRLDAALAARIKEASR 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2392 HLGVSPgvlFHV---AWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISLaDRGA--AEVVERTSHD 2466
Cdd:cd19532   236 KLRVTP---FHFylaALQVLLARLLDVDDICIGIADANR--TDEDFMETIGFFLNLLPLRFRR-DPSQtfADVLKETRDK 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2467 LMTLLEHEQAPLAL------AQRCSGvapPMPLFSTLLNYRhtQASSTDNTLSDIR---VLTSEERTNYPLTLAVDDRGE 2537
Cdd:cd19532   310 AYAALAHSRVPFDVlldelgVPRSAT---HSPLFQVFINYR--QGVAESRPFGDCElegEEFEDARTPYDLSLDIIDNPD 384


                  ....*
gi 641744967 2538 GFSLV 2542
Cdd:cd19532   385 GDCLL 389
PrpE cd05967
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ...
 1536-2021 7.79e-33

Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.


Pssm-ID: 341271 [Multi-domain]  Cd Length: 617  Bit Score: 137.83  E-value: 7.79e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1536 DQAARTPDTtAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAE 1615
Cdd:cd05967    66 DQIALIYDS-PVTGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAK 144

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1616 RLAYMLDDASPVALLTqAN--------------------------------QRALLTGDVPRIL--LDTADFSHLSEDNP 1661
Cdd:cd05967   145 ELASRIDDAKPKLIVT-AScgiepgkvvpykplldkalelsghkphhvlvlNRPQVPADLTKPGrdLDWSELLAKAEPVD 223

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1662 HVPgLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVW-MQNTYRLTPDDrvlqktpfsfdvsVW----EFFW------ 1730
Cdd:cd05967   224 CVP-VAATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWsMRNIYGIKPGD-------------VWwaasDVGWvvghsy 289

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1731 ----PLLYGARLVM--ARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQwADAD------CACDSLRRVICSGEALP 1798
Cdd:cd05967   290 ivygPLLHGATTVLyeGKPVGTPDPGAFWRVIEKYQVNALFTAPTAIRAIRK-EDPDgkyikkYDLSSLRTLFLAGERLD 368

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1799 AELQQRFFARFNAQLHNLYGPTEaaidvTFWA--CQPddhRSFVPI-------GRPIANTQLYILDTLGQPVPLGVAGEL 1869
Cdd:cd05967   369 PPTLEWAENTLGVPVIDHWWQTE-----TGWPitANP---VGLEPLpikagspGKPVPGYQVQVLDEDGEPVGPNELGNI 440

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1870 HIGGvGVARGYLNRPDLTAERFIPDPFINQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCP 1949
Cdd:cd05967   441 VIKL-PLPPGCLLTLWKNDERFKKLYLSKFPG--YYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHP 517

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 1950 GVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVP-GAF---VTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05967   518 AVAECAVVGVRDELKGQVPLGLVVLKEGVKITAEELEKELVALVREQIGPvAAFrlvIFVKRLPKTRSGKILRRTL 593
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
 487-957 1.39e-32

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 135.12  E-value: 1.39e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:cd12118    18 PDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEEIAFIL 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALL--TQSAQVAQLNSTLPTVLLDTPAAaacpDTNPVVqglhaahlayVIYTSGSTGRPKGVMVAHRN--VINL 642
Cdd:cd12118    98 RHSEAKVLFvdREFEYEDLLAEGDPDFEWIPPAD----EWDPIA----------LNYTSGTTGRPKGVVYHHRGayLNAL 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  643 ATGLHTLLaldHPSRIALNASIVFDAS--VKNWIQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLD 720
Cdd:cd12118   164 ANILEWEM---KQHPVYLWTLPMFHCNgwCFPWTVAAVGGTNVCLR---KVDAKAIYDLIEKHKVTHFCGAPTVLNMLAN 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  721 AG-LGSDPAYQPAQVLIGGEAISPAVWSRLQSLSdTRFINVYGPTECTVDATACVvdrTQP----LPTIGKPLANTR--- 792
Cdd:cd12118   238 APpSDARPLPHRVHVMTAGAPPPAAVLAKMEELG-FDVTHVYGLTETYGPATVCA---WKPewdeLPTEERARLKARqgv 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  793 -------LYILDAQ-DQPVPI-GVT-GELHIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLARWLPDGNID 862
Cdd:cd12118   314 ryvgleeVDVLDPEtMKPVPRdGKTiGEIVFRGNIVMKGYLKNPEATAEAF---------RGGWFHSGDLAVIHPDGYIE 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  863 YLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPS 942
Cdd:cd12118   385 IKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEEEIIAFCREHLAGFMVPK 464

                         490
                  ....*....|....*
gi 641744967  943 AFVTLDaLPLTPNGK 957
Cdd:cd12118   465 TVVFGE-LPKTSTGK 478
beta-lac_NRPS cd19547
Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis ...
 28-434 1.99e-32

Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis NocB which exhibits an unusual cyclization to form beta-lactam rings in pro-nocardicin G synthesis; Nocardia uniformis NRPS NocB acts centrally in the biosynthesis of the nocardicin monocyclic beta-lactam antibiotics. Along with another NRPS NocA, it mediates an unusual cyclization to form beta-lactam rings in the synthesis of the beta-lactam-containing pentapeptide pro-nocardicin G. This small subfamily is related to DCL-type Condensation (C) domains, which catalyze condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; domains belonging to this subfamily have an HHHxxxD motif at the active site.


Pssm-ID: 380469 [Multi-domain]  Cd Length: 422  Bit Score: 133.21  E-value: 1.99e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   28 IYPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHQPVQVVWR--QAPLT 105
Cdd:cd19547     1 VYPLAPMQEGMLFRGLFWPDSDAYFNQNVLELVGGTDEDVLREAWRRVADRYEILRTGFTWRDRAEPLQYVRDdlAPPWA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  106 VNTLTTTSSDTVPAQL-RAATDPSNHRLNLSNAPLLSATTAHDPVCGEWLLsLSIHHLISDHITQALIIDEIRLLLED-- 182
Cdd:cd19547    81 LLDWSGEDPDRRAELLeRLLADDRAAGLSLADCPLYRLTLVRLGGGRHYLL-WSHHHILLDGWCLSLIWGDVFRVYEEla 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  183 --RPEALPKPLPYRNFIAQIL--SVPLSEHEQYFRNRLADIdTPTaPFDLVDVQGNGEDITEARlSLDSSLADALRRQAR 258
Cdd:cd19547   160 hgREPQLSPCRPYRDYVRWIRarTAQSEESERFWREYLRDL-TPS-PFSTAPADREGEFDTVVH-EFPEQLTRLVNEAAR 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  259 HLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLGADRVMGMFINTLPLRVSLR-ERSVHDVVQATSHELMML 337
Cdd:cd19547   237 GYGVTTNAISQAAWSMLLALQTGARDVVHGLTIAGRPPELEGSEHMVGIFINTIPLRIRLDpDQTVTGLLETIHRDLATT 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  338 LAHEQAPLALAQQCS--------QVPPPLPLFSTLFNYRHSQKDASSQFWEgmrqLSGRERTNYPITLSV---DDLGDGF 406
Cdd:cd19547   317 AAHGHVPLAQIKSWAsgerlsggRVFDNLVAFENYPEDNLPGDDLSIQIID----LHAQEKTEYPIGLIVlplQKLAFHF 392

                         410       420
                  ....*....|....*....|....*...
gi 641744967  407 NLTAKTVMGVDPERIVHYMLTAIENLVT 434
Cdd:cd19547   393 NYDTTHFTRAQVDRFIEVFRLLTEQLCR 420
FACL_like_5 cd05924
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 618-959 3.22e-32

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341248 [Multi-domain]  Cd Length: 364  Bit Score: 130.97  E-value: 3.22e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  618 YVIYTSGSTGRPKGVMVAHR-------NVINLATGLHTLLALDHPSRIAlNASIVF--------DASVKNWIQLLSGHTL 682
Cdd:cd05924     7 YILYTGGTTGMPKGVMWRQEdifrmlmGGADFGTGEFTPSEDAHKAAAA-AAGTVMfpapplmhGTGSWTAFGGLLGGQT 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  683 VLVPDaLRADAHQLWRYFARHAVDLFDCT-PVQLQWLLDAGLGSDPAYQPAQVLI--GGEAISPAVWSRLQ-SLSDTRFI 758
Cdd:cd05924    86 VVLPD-DRFDPEEVWRTIEKHKVTSMTIVgDAMARPLIDALRDAGPYDLSSLFAIssGGALLSPEVKQGLLeLVPNITLV 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  759 NVYGPTEctVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAG-VARGYLHRPDLTAERFipdp 837
Cdd:cd05924   165 DAFGSSE--TGFTGSGHSAGSGPETGPFTRANPDTVVLDDDGRVVPPGSGGVGWIARRGhIPLGYYGDEAKTAETF---- 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  838 FSADpAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCAR 917
Cdd:cd05924   239 PEVD-GVRYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQEVVAVVQLR 317

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 641744967  918 PDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLD 959
Cdd:cd05924   318 EGAGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGKAD 359
PRK06060 PRK06060
p-hydroxybenzoic acid--AMP ligase FadD22;
1554-2023 6.10e-32

p-hydroxybenzoic acid--AMP ligase FadD22;


Pssm-ID: 180374 [Multi-domain]  Cd Length: 705  Bit Score: 135.93  E-value: 6.10e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA 1633
Cdd:PRK06060   31 VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALAARNTEPALVVTSD 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 NQRALLTgdvPRILLDTAD-FSHLSEDNP---HVPGLDAHhlAYVIYTSGSTGKPKGVMNSH-------RALCnrlvwmQ 1702
Cdd:PRK06060  111 ALRDRFQ---PSRVAEAAElMSEAARVAPggyEPMGGDAL--AYATYTSGTTGPPKAAIHRHadpltfvDAMC------R 179

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1703 NTYRLTPDDRVLQKTPFSFDV----SVWeffWPLLYGARLVMAR-PDGHKDAAYLAQLIERTgitTLHFVPSMlqqFVQW 1777
Cdd:PRK06060  180 KALRLTPEDTGLCSARMYFAYglgnSVW---FPLATGGSAVINSaPVTPEAAAILSARFGPS---VLYGVPNF---FARV 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1778 ADAdCACDSLRRVIC---SGEALPAELQQRFfARFNAQLHNLYGPTEAAIDVTFWACQPDDHRSFVpIGRPIANTQLYIL 1854
Cdd:PRK06060  251 IDS-CSPDSFRSLRCvvsAGEALELGLAERL-MEFFGGIPILDGIGSTEVGQTFVSNRVDEWRLGT-LGRVLPPYEIRVV 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1855 DTLGQPVPLGVAGELHIGGVGVARGYLNRPDltaerfipdPFINQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGF 1934
Cdd:PRK06060  328 APDGTTAGPGVEGDLWVRGPAIAKGYWNRPD---------SPVANEG--WLDTRDRVCIDSDGWVTYRCRADDTEVIGGV 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1935 RIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDAFPLT 2011
Cdd:PRK06060  397 NVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSvmrDLHRGLLNRLSAFKVPHRFAVVDRLPRT 476

                         490
                  ....*....|..
gi 641744967 2012 PNGKLDRKALPA 2023
Cdd:PRK06060  477 PNGKLVRGALRK 488
PRK07514 PRK07514
malonyl-CoA synthase; Validated
 2620-3097 1.19e-31

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 132.31  E-value: 1.19e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAV-VFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:PRK07514   16 RDAPFIeTPDGLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAELDYF 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISQSAHLGIMN------GSLPVILLDDGETRPF----DNEPDTPLDARKQgltPRHLAYVIYTSGSTGKPK 2768
Cdd:PRK07514   96 IGDAEPALVVCDPANFAWLSkiaaaaGAPHVETLDADGTGSLleaaAAAPDDFETVPRG---ADDLAAILYTSGTTGRSK 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2769 GVMVEHANMVNFLCSMRKEPGIAQEDVLlgvtslsfdISILEIF----------LPLLNGARLILATQAQAADAQQlamL 2838
Cdd:PRK07514  173 GAMLSHGNLLSNALTLVDYWRFTPDDVL---------IHALPIFhthglfvatnVALLAGASMIFLPKFDPDAVLA---L 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2839 IERhAVSFMqATPSTW-RMLVElrdfalpPGF-KALCG-------GEA--LPENLAT-------ALLQKvttlwnlYGPT 2900
Cdd:PRK07514  241 MPR-ATVMM-GVPTFYtRLLQE-------PRLtREAAAhmrlfisGSAplLAETHREfqertghAILER-------YGMT 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2901 ETtiwstlnGLTTPTPY--------IGHPIANTQIYILD-AQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDP 2971
Cdd:PRK07514  305 ET-------NMNTSNPYdgerragtVGFPLPGVSLRVTDpETGAELPPGEIGMIEVKGPNVFKGYWRMPEKTAEEFRADG 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2972 FsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQ 3051
Cdd:PRK07514  378 F--------FITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEGVTAVVVPK 449

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 3052 PDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK07514  450 PGAALDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLL 495
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
 442-960 1.59e-31

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 132.97  E-value: 1.59e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  442 QPALSQPILPKSERQQVLVD-FNATDADFPREmliqqrfeaqaeqtpEAIAVLFEDQHLTYRELNRRANQLAHHLIALGV 520
Cdd:PRK12583    3 QPSYYQGGGDKPLLTQTIGDaFDATVARFPDR---------------EALVVRHQALRYTWRQLADAVDRLARGLLALGV 67

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  521 QPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLT-----QSAQVAQLNSTLPTVLLDT 595
Cdd:PRK12583   68 QPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWVICadafkTSDYHAMLQELLPGLAEGQ 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  596 PAAAACP-----------------------------------DTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVI 640
Cdd:PRK12583  148 PGALACErlpelrgvvslapapppgflawhelqargetvsreALAERQASLDRDDPINIQYTSGTTGFPKGATLSHHNIL 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  641 NLATGLHTLLALDHPSRIALNASI--VFDASVKNWIQLLSGHTLVLVPDALRADAhqlwryfARHAVDLFDCTpvqlqwl 718
Cdd:PRK12583  228 NNGYFVAESLGLTEHDRLCVPVPLyhCFGMVLANLGCMTVGACLVYPNEAFDPLA-------TLQAVEEERCT------- 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  719 ldaGLGSDPAYQPAQV----------------LIGGEAISPAVWSR-LQSLSDTRFINVYGPTECT-VDATACVVDrtqP 780
Cdd:PRK12583  294 ---ALYGVPTMFIAELdhpqrgnfdlsslrtgIMAGAPCPIEVMRRvMDEMHMAEVQIAYGMTETSpVSLQTTAAD---D 367

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  781 LP----TIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAErfipdpfSADPAARIYkTGDLARWL 856
Cdd:PRK12583  368 LErrveTVGRTQPHLEVKVVDPDGATVPRGEIGELCTRGYSVMKGYWNNPEATAE-------SIDEDGWMH-TGDLATMD 439

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  857 PDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLA 936
Cdd:PRK12583  440 EQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASEEELREFCKARIA 519

                         570       580
                  ....*....|....*....|....
gi 641744967  937 DYMIPSAFVTLDALPLTPNGKLDR 960
Cdd:PRK12583  520 HFKVPRYFRFVDEFPMTVTGKVQK 543
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
2608-3097 1.65e-31

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 132.70  E-value: 1.65e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGE--ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVP 2685
Cdd:PRK05852   18 IADLVEVAATRLPEAPALVVTAdrIAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVP 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2686 LDPTYPVERL---------RYMLDDAK-------------PVAlISQSAHLGIMNGSLPVILLDDGETrpfdnepdTPLD 2743
Cdd:PRK05852   98 LDPALPIAEQrvrsqaagaRVVLIDADgphdraepttrwwPLT-VNVGGDSGPSGGTLSVHLDAATEP--------TPAT 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2744 ARKQGLTPRHlAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLIL 2823
Cdd:PRK05852  169 STPEGLRPDD-AMIMFTGGTTGLPKMVPWTHANIASSVRAIITGYRLSPRDATVAVMPLYHGHGLIAALLATLASGGAVL 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2824 ATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELRDFALPPG------FKALCGGEALPENLATALLQKVTTLWNLY 2897
Cdd:PRK05852  248 LPARGRFSAHTFWDDIKAVGATWYTAVPTIHQILLERAATEPSGRkpaalrFIRSCSAPLTAETAQALQTEFAAPVVCAF 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2898 GPTETTIWSTlnglTTPTPYIGH---PIANT---------QIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAE 2965
Cdd:PRK05852  328 GMTEATHQVT----TTQIEGIGQtenPVVSTglvgrstgaQIRIVGSDGLPLPAGAVGEVWLRGTTVVRGYLGDPTITAA 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2966 RFiTDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLV 3045
Cdd:PRK05852  404 NF-TDGW--------LRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVA 474

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967 3046 AYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK05852  475 AVIVPRESAPPTAEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAV 526
PRK07787 PRK07787
acyl-CoA synthetase; Validated
 2611-3100 2.04e-31

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 131.27  E-value: 2.04e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2611 LFEAQVACTPD-AIAVVFGEASLSYDELNRRANRLAHhlisfGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPT 2689
Cdd:PRK07787    4 LNPAAVAAAADiADAVRIGGRVLSRSDLAGAATAVAE-----RVAGARRVAVLATPTLATVLAVVGALIAGVPVVPVPPD 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2690 YPVERLRYMLDDAKPVALIsqsahlgimnGSLPvillDDGETRP-FDNEPDTPLDARKQGLTPRHLAYVIYTSGSTGKPK 2768
Cdd:PRK07787   79 SGVAERRHILADSGAQAWL----------GPAP----DDPAGLPhVPVRLHARSWHRYPEPDPDAPALIVYTSGTTGPPK 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2769 GVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLsFDIS--ILEIFLPLLNGARLilatqaqaadaqqlamlieRHAVSF 2846
Cdd:PRK07787  145 GVVLSRRAIAADLDALAEAWQWTADDVLVHGLPL-FHVHglVLGVLGPLRIGNRF-------------------VHTGRP 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2847 ---------------MQATPSTWRMLVELRDF--ALPPGFKALCGGEALP----ENLATALLQKVTtlwNLYGPTETTIW 2905
Cdd:PRK07787  205 tpeayaqalseggtlYFGVPTVWSRIAADPEAarALRGARLLVSGSAALPvpvfDRLAALTGHRPV---ERYGMTETLIT 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2906 STLNGLTTPTP-YIGHPIANTQIYILDAQGRVVPLGVA--GEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYK 2982
Cdd:PRK07787  282 LSTRADGERRPgWVGLPLAGVETRLVDEDGGPVPHDGEtvGELQVRGPTLFDGYLNRPDATAAAFTADGW--------FR 353

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2983 TGDLGRWLPDGTLEYLGRN--DFqVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTvlEPAD 3060
Cdd:PRK07787  354 TGDVAVVDPDGMHRIVGREstDL-IKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDV--AADE 430

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967 3061 LRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAP 3100
Cdd:PRK07787  431 LIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLLSE 470
Ac_CoA_lig_AcsA TIGR02188
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ...
 1534-2035 2.46e-31

acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.


Pssm-ID: 274022 [Multi-domain]  Cd Length: 626  Bit Score: 133.14  E-value: 2.46e-31
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1534 VEDQAARTPDTTAVLFE------DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVP 1607
Cdd:TIGR02188   63 VDRHLEARPDKVAIIWEgdepgeVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSV 142

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1608 LDPGYPAERLAYMLDDASPVALLTqANQ-----RAL-LTGDVPRILLDTAD-------FSHLSEDNPH-VPGLD------ 1667
Cdd:TIGR02188  143 VFGGFSAEALADRINDAGAKLVIT-ADEglrggKVIpLKAIVDEALEKCPVsvehvlvVRRTGNPVVPwVEGRDvwwhdl 221

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1668 --------------AHHLAYVIYTSGSTGKPKGVMNSHRALcnrLVWMQNTYRLTpddrvlqktpfsFDVSVWEFFW--- 1730
Cdd:TIGR02188  222 makasaycepepmdSEDPLFILYTSGSTGKPKGVLHTTGGY---LLYAAMTMKYV------------FDIKDGDIFWcta 286

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1731 --------------PLLYGARLVM--ARPDgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCAC---DSLrRVI 1791
Cdd:TIGR02188  287 dvgwitghsyivygPLANGATTVMfeGVPT-YPDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKhdlSSL-RLL 364

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1792 CS-GEALPAELQQRFfarfnaqlHNLYGPTEAAIDVTFWACQPDDHR-----SFVPI-----GRPIANTQLYILDTLGQP 1860
Cdd:TIGR02188  365 GSvGEPINPEAWMWY--------YKVVGKERCPIVDTWWQTETGGIMitplpGATPTkpgsaTLPFFGIEPAVVDEEGNP 436

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1861 VP-LGVAGELHIGGV--GVARGYLNRPdltaERFIPDPFINQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIE 1937
Cdd:TIGR02188  437 VEgPGEGGYLVIKQPwpGMLRTIYGDH----ERFVDTYFSPFPG--YYFTGDGARRDKDGYIWITGRVDDVINVSGHRLG 510

                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  1938 LGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPaDLRQQLGQHLAEYMVPGA------FVtlDAFPLT 2011
Cdd:TIGR02188  511 TAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDD-ELRKELRKHVRKEIGPIAkpdkirFV--PGLPKT 587

                          570       580
                   ....*....|....*....|....
gi 641744967  2012 PNGKLDRKALpapdqSAVATRDYE 2035
Cdd:TIGR02188  588 RSGKIMRRLL-----RKIAAGEAE 606
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
 497-899 2.75e-31

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 130.56  E-value: 2.75e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  497 QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLT 576
Cdd:cd17640     4 KRITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVV 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  577 QSAqvaqlnstlptvlldtpaaaacPDTnpvvqglhaahLAYVIYTSGSTGRPKGVMVAHRNVI----NLATGLHT---- 648
Cdd:cd17640    84 END----------------------SDD-----------LATIIYTSGTTGNPKGVMLTHANLLhqirSLSDIVPPqpgd 130

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  649 ----LLALDHPSR-------IALNASIVFDA--SVKNWIQLLSGHTLVLVP---DALRADAHQLWRyfarhavdlfDCTP 712
Cdd:cd17640   131 rflsILPIWHSYErsaeyfiFACGCSQAYTSirTLKDDLKRVKPHYIVSVPrlwESLYSGIQKQVS----------KSSP 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  713 VQLQWLLDAGLGSdpayQPAQVLIGGEAISPAVwsrlqslsDTRF-------INVYGPTECTVDATAcvvdRTQPLP--- 782
Cdd:cd17640   201 IKQFLFLFFLSGG----IFKFGISGGGALPPHV--------DTFFeaigievLNGYGLTETSPVVSA----RRLKCNvrg 264

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  783 TIGKPLANTRLYILDAQ-DQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNI 861
Cdd:cd17640   265 SVGRPLPGTEIKIVDPEgNVVLPPGEKGIVWVRGPQVMKGYYKNPEATSKVLDSDGW--------FNTGDLGWLTCGGEL 336

                         410       420       430
                  ....*....|....*....|....*....|....*....
gi 641744967  862 DYLGRNDFQIKVR-GFRIEAGEIESRLLRCPGVQDAVVI 899
Cdd:cd17640   337 VLTGRAKDTIVLSnGENVEPQPIEEALMRSPFIEQIMVV 375
FACL_like_5 cd05924
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1673-2017 5.43e-31

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341248 [Multi-domain]  Cd Length: 364  Bit Score: 127.50  E-value: 5.43e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1673 YVIYTSGSTGKPKGVMNSH----RALCNRLVWMQNTYrlTPDDRVLQKT------------PFSFDVSVWEFFWPLLYGA 1736
Cdd:cd05924     7 YILYTGGTTGMPKGVMWRQedifRMLMGGADFGTGEF--TPSEDAHKAAaaaagtvmfpapPLMHGTGSWTAFGGLLGGQ 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1737 RLVMarPDGHKDAAYLAQLIERTGITTLHFV------PsMLQQFVQWADADCAcdSLRRVICSGEALPAELQQRFFARF- 1809
Cdd:cd05924    85 TVVL--PDDRFDPEEVWRTIEKHKVTSMTIVgdamarP-LIDALRDAGPYDLS--SLFAISSGGALLSPEVKQGLLELVp 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1810 NAQLHNLYGPTEAAIDVT-FWACQPDDHRSFVpigrpIANTQLYILDTLGQPVPLGVAGELHIGGVG-VARGYLNRPDLT 1887
Cdd:cd05924   160 NITLVDAFGSSETGFTGSgHSAGSGPETGPFT-----RANPDTVVLDDDGRVVPPGSGGVGWIARRGhIPLGYYGDEAKT 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1888 AERFipdPFINqpGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTR 1967
Cdd:cd05924   235 AETF---PEVD--GVRYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQE 309

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|
gi 641744967 1968 LVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:cd05924   310 VVAVVQLREGAGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGKAD 359
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
 2632-3101 5.60e-31

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 129.16  E-value: 5.60e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISqs 2711
Cdd:cd05969     1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLIT-- 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2712 ahlgimngslpvillddgetrpfdnepdTPLDARKqgLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIA 2791
Cdd:cd05969    79 ----------------------------TEELYER--TDPEDPTLLHYTSGTTGTPKGVLHVHDAMIFYYFTGKYVLDLH 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2792 QEDVLLG------VTSLSFDIsileiFLPLLNGARLILATQAQAADAQQLAmlIERHAVSFMQATPSTWRML----VEL- 2860
Cdd:cd05969   129 PDDIYWCtadpgwVTGTVYGI-----WAPWLNGVTNVVYEGRFDAESWYGI--IERVKVTVWYTAPTAIRMLmkegDELa 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2861 RDFALPPGFKALCGGEAL-PENLATALLQKVTTLWNLYGPTETTIWSTLNGLTTPTP--YIGHPIANTQIYILDAQGRVV 2937
Cdd:cd05969   202 RKYDLSSLRFIHSVGEPLnPEAIRWGMEVFGVPIHDTWWQTETGSIMIANYPCMPIKpgSMGKPLPGVKAAVVDENGNEL 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2938 PLGVAGEIHIAGA--GVVRGYLGRPDLTAERFITDpfsgapearMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELG 3015
Cdd:cd05969   282 PPGTKGILALKPGwpSMFRGIWNDEERYKNSFIDG---------WYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPF 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3016 EIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDtvLEPAD-----LRQRLSEGVAEYMIPSAFVTLDAFPLTPNG 3090
Cdd:cd05969   353 EVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEG--FEPSDelkeeIINFVRQKLGAHVAPREIEFVDNLPKTRSG 430

                         490
                  ....*....|.
gi 641744967 3091 KLDRKALPAPD 3101
Cdd:cd05969   431 KIMRRVLKAKE 441
PRK09088 PRK09088
acyl-CoA synthetase; Validated
 482-963 6.52e-31

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 129.93  E-value: 6.52e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  482 QAEQTPEAIAV--LFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPA 559
Cdd:PRK09088    4 HARLQPQRLAAvdLALGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSA 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  560 ERLAYILDDAAPvALLTQSAQVAQLnSTLPTVLLDTPAAAAC--PDTNPvvqGLHAAHLAYVIYTSGSTGRPKGVMVAHR 637
Cdd:PRK09088   84 SELDALLQDAEP-RLLLGDDAVAAG-RTDVEDLAAFIASADAlePADTP---SIPPERVSLILFTSGTSGQPKGVMLSER 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  638 NVINLATGLHTLLALDHPSRIALNAS----IVFDASVKNwiQLLSGHTlVLVPDALRADAHQLWRYFARHAVDLFDCTPv 713
Cdd:PRK09088  159 NLQQTAHNFGVLGRVDAHSSFLCDAPmfhiIGLITSVRP--VLAVGGS-ILVSNGFEPKRTLGRLGDPALGITHYFCVP- 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  714 QLQWLLDAGLGSDP-AYQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTEC-TV---DATACVVDRTQPLPTIGKPL 788
Cdd:PRK09088  235 QMAQAFRAQPGFDAaALRHLTALFTGGAPHAAEDILGWLDDGIPMVDGFGMSEAgTVfgmSVDCDVIRAKAGAAGIPTPT 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  789 ANTRlyILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRND 868
Cdd:PRK09088  315 VQTR--VVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAFTGDGW--------FRTGDIARRDADGFFWVVDRKK 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  869 FQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLD 948
Cdd:PRK09088  385 DMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDLERIRSHLSTRLAKYKVPKHLRLVD 464

                         490
                  ....*....|....*
gi 641744967  949 ALPLTPNGKLDRKAL 963
Cdd:PRK09088  465 ALPRTASGKLQKARL 479
PRK06145 PRK06145
acyl-CoA synthetase; Validated
 483-963 8.24e-31

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 130.01  E-value: 8.24e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:PRK06145   12 ARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEV 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLTQSAQVAQLNSTLPTVLLDtpaAAACPDTNPVVQGLHAAHLAYV---------IYTSGSTGRPKGVM 633
Cdd:PRK06145   92 AYILGDAGAKLLLVDEEFDAIVALETPKIVID---AAAQADSRRLAQGGLEIPPQAAvaptdlvrlMYTSGTTDRPKGVM 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  634 VAHRNVinlatglhTLLALDHPSRIALNAS---IVFDASVKNWIQLLSGHTLVLVPDALRA----DAHQLWRYFARHAVD 706
Cdd:PRK06145  169 HSYGNL--------HWKSIDHVIALGLTASerlLVVGPLYHVGAFDLPGIAVLWVGGTLRIhrefDPEAVLAAIERHRLT 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  707 LFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAvwSRLQSLSD----TRFINVYGPTE-CTVDATACVVDRTQPL 781
Cdd:PRK06145  241 CAWMAPVMLSRVLTVPDRDRFDLDSLAWCIGGGEKTPE--SRIRDFTRvftrARYIDAYGLTEtCSGDTLMEAGREIEKI 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  782 PTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPDGNI 861
Cdd:PRK06145  319 GSTGRALAHVEIRIADGAGRWLPPNMKGEICMRGPKVTKGYWKDPEKTAEAFYGDWF---------RSGDVGYLDEEGFL 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  862 DYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIP 941
Cdd:PRK06145  390 YLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATLTLEALDRHCRQRLASFKVP 469

                         490       500
                  ....*....|....*....|..
gi 641744967  942 SAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK06145  470 RQLKVRDELPRNPSGKVLKRVL 491
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
 1534-1999 1.77e-30

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 130.38  E-value: 1.77e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1534 VEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYP 1613
Cdd:PRK08279   43 FEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQR 122

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1614 AERLAYMLDDASPVALLTQANQ-------RALLTGDVPRILLDTADF-------------SHLSEDNPHV-PGLDAHHLA 1672
Cdd:PRK08279  123 GAVLAHSLNLVDAKHLIVGEELveafeeaRADLARPPRLWVAGGDTLddpegyedlaaaaAGAPTTNPASrSGVTAKDTA 202

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1673 YVIYTSGSTGKPK-GVMNSHRALCNrLVWMQNTYRLTPDDRVLQKTPFSFDV-------SVweffwpLLYGARLVMAR-- 1742
Cdd:PRK08279  203 FYIYTSGTTGLPKaAVMSHMRWLKA-MGGFGGLLRLTPDDVLYCCLPLYHNTggtvawsSV------LAAGATLALRRkf 275

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1743 ------PDghkdaaylaqlIERTGITTLHFVPSM----LQQFVQWADADcacDSLRRVIcsGEALPAELQQRFFARFN-A 1811
Cdd:PRK08279  276 sasrfwDD-----------VRRYRATAFQYIGELcrylLNQPPKPTDRD---HRLRLMI--GNGLRPDIWDEFQQRFGiP 339

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1812 QLHNLYGPTEAaiDVTFW-------ACqpddhrSFVPI--GRPIA------NTQLYILDTLG--QPVPLGVAGELhIGGV 1874
Cdd:PRK08279  340 RILEFYAASEG--NVGFInvfnfdgTV------GRVPLwlAHPYAivkydvDTGEPVRDADGrcIKVKPGEVGLL-IGRI 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1875 GVAR---GYlNRPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGR--NDFQVKlrGFRIELGEIEARLMQCP 1949
Cdd:PRK08279  411 TDRGpfdGY-TDPEASEKKILRDVF--KKGDAWFNTGDLMRDDGFGHAQFVDRlgDTFRWK--GENVATTEVENALSGFP 485

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967 1950 GVQEAVV--VAREDSPGDTRLVAyLCPQPGVTPDPADLRQQLGQHLAEYMVP 1999
Cdd:PRK08279  486 GVEEAVVygVEVPGTDGRAGMAA-IVLADGAEFDLAALAAHLYERLPAYAVP 536
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
467-963 2.74e-30

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 129.40  E-value: 2.74e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  467 ADFPREMLIQQ------RFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVG 539
Cdd:PRK08974   11 ADVPAEINPDRyqslvdMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQnGLGLKKGDRVALMMPNLLQYPIA 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  540 LLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTqsaqVAQLNSTLPTVLLDTP-------------AAAACPDTNP 606
Cdd:PRK08974   91 LFGILRAGMIVVNVNPLYTPRELEHQLNDSGAKAIVI----VSNFAHTLEKVVFKTPvkhviltrmgdqlSTAKGTLVNF 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  607 VV-------------------QGLHAAH-------------LAYVIYTSGSTGRPKGVMVAHRNVI-NLATGLHTLLALD 653
Cdd:PRK08974  167 VVkyikrlvpkyhlpdaisfrSALHKGRrmqyvkpelvpedLAFLQYTGGTTGVAKGAMLTHRNMLaNLEQAKAAYGPLL 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  654 HPSR----IALNASIVFDASVKNWIQLLSGHTLVLV--PDALRADAHQLWRY--FARHAVD-LF-------DCTPVQLQW 717
Cdd:PRK08974  247 HPGKelvvTALPLYHIFALTVNCLLFIELGGQNLLItnPRDIPGFVKELKKYpfTAITGVNtLFnallnneEFQELDFSS 326

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  718 L-LDAGlgsdpayqpaqvliGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYIL 796
Cdd:PRK08974  327 LkLSVG--------------GGMAVQQAVAERWVKLTGQYLLEGYGLTECSPLVSVNPYDLDYYSGSIGLPVPSTEIKLV 392

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  797 DAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAErFIPDPFSAdpaariykTGDLARWLPDGNIDYLGRNDFQIKVRGF 876
Cdd:PRK08974  393 DDDGNEVPPGEPGELWVKGPQVMLGYWQRPEATDE-VIKDGWLA--------TGDIAVMDEEGFLRIVDRKKDMILVSGF 463

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  877 RIEAGEIESRLLRCPGVQDAVVIARE-DSPGDTrlVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPN 955
Cdd:PRK08974  464 NVYPNEIEDVVMLHPKVLEVAAVGVPsEVSGEA--VKIFVVKKDPSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNV 541


                  ....*...
gi 641744967  956 GKLDRKAL 963
Cdd:PRK08974  542 GKILRREL 549
LCL_NRPS-like cd19531
LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar ...
 2162-2542 2.87e-30

LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar domains including the C-domain of SgcC5, a free-standing NRPS with both ester- and amide- bond forming activity; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. Streptomyces globisporus SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380454 [Multi-domain]  Cd Length: 427  Bit Score: 126.70  E-value: 2.87e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2162 YPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDlSQPVQVVWRQAILPINH 2241
Cdd:cd19531     2 LPLSFAQQRLWFLDQLEPGSAAYNIPGALRLRGPLDVAALERALNELVARHEALRTTFVEVD-GEPVQVILPPLPLPLPV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2242 --FEPTSPEDVLAQLQAHTEPRTRR-IDLSQAPLFRADIahdpLQ---NEWLLALSFHHLISDHMTLALIVGEIRLL--- 2312
Cdd:cd19531    81 vdLSGLPEAEREAEAQRLAREEARRpFDLARGPLLRATL----LRlgeDEHVLLLTMHHIVSDGWSMGVLLRELAALyaa 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2313 -LQHQADALPtPLP--YRNFIA---QTLSVPN-SAHEAYFRDKLADVDEPtapfglLN---------VQGSGGDIHeaRL 2376
Cdd:cd19531   157 fLAGRPSPLP-PLPiqYADYAVwqrEWLQGEVlERQLAYWREQLAGAPPV------LElptdrprpaVQSFRGARV--RF 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2377 VLDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGR-LAGAEGadrIMGMFINTLPLRISLA-DR 2454
Cdd:cd19531   228 TLPAELTAALRALARREGATLFMTLLAAFQVLLHRYSGQDDIVVGTPVAGRnRAELEG---LIGFFVNTLVLRTDLSgDP 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2455 GAAEVVERTSHDLMTLLEHEQAPL-----ALaqrcsgvAPP-----MPLFSTLLNYRHTQASSTDNTLSDIRVLTSEERT 2524
Cdd:cd19531   305 TFRELLARVRETALEAYAHQDLPFeklveAL-------QPErdlsrSPLFQVMFVLQNAPAAALELPGLTVEPLEVDSGT 377

                         410
                  ....*....|....*....
gi 641744967 2525 -NYPLTLAVDDRGEGFSLV 2542
Cdd:cd19531   378 aKFDLTLSLTETDGGLRGS 396
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
 2611-3097 3.02e-30

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 128.72  E-value: 3.02e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2611 LFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAI-CVERG--LDMVVGLLGIlkaGGAYVPLD 2687
Cdd:PRK06155   26 MLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALmCGNRIefLDVFLGCAWL---GAIAVPIN 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2688 PTYPVERLRYMLDDAKPVALISQSAHLGIMNG------SLPVILLDDGET-----RPFDNEPDTPLDAR--KQGLTPRHL 2754
Cdd:PRK06155  103 TALRGPQLEHILRNSGARLLVVEAALLAALEAadpgdlPLPAVWLLDAPAsvsvpAGWSTAPLPPLDAPapAAAVQPGDT 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2755 AYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLsFDISILEIFLP-LLNGARLILATQAQAADAQ 2833
Cdd:PRK06155  183 AAILYTSGTTGPSKGVCCPHAQFYWWGRNSAEDLEIGADDVLYTTLPL-FHTNALNAFFQaLLAGATYVLEPRFSASGFW 261

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2834 QLamlIERHA----------VSFMQATPSTWRMLVELRDFALPPGFKALCgGEALPENLATALLQKvttlwnlYGPTETT 2903
Cdd:PRK06155  262 PA---VRRHGatvtyllgamVSILLSQPARESDRAHRVRVALGPGVPAAL-HAAFRERFGVDLLDG-------YGSTETN 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2904 IwstLNGLTTPTP---YIGHPIANTQIYILDAQGRVVPLGVAGEIhiagagVVR---------GYLGRPDLTAErfitdp 2971
Cdd:PRK06155  331 F---VIAVTHGSQrpgSMGRLAPGFEARVVDEHDQELPDGEPGEL------LLRadepfafatGYFGMPEKTVE------ 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2972 fsgAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQ 3051
Cdd:PRK06155  396 ---AWRNLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLR 472

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 3052 PDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK06155  473 DGTALEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVL 518
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
 499-963 3.26e-30

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 129.11  E-value: 3.26e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIAL-GVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQ 577
Cdd:PRK05677   50 LTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKALVCL 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  578 S--------------------AQVAQLNSTLPTVLLDT--------------PAAAACPDTNPVVQG-------LHAAHL 616
Cdd:PRK05677  130 AnmahlaekvlpktgvkhvivTEVADMLPPLKRLLINAvvkhvkkmvpayhlPQAVKFNDALAKGAGqpvteanPQADDV 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  617 AYVIYTSGSTGRPKGVMVAHRNVI------------NLATGLHTLLA---LDHPSRIALNASIVfdasvknwiqLLSGHT 681
Cdd:PRK05677  210 AVLQYTGGTTGVAKGAMLTHRNLVanmlqcralmgsNLNEGCEILIAplpLYHIYAFTFHCMAM----------MLIGNH 279

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  682 LVLVPDALRADAH----QLWRYFArhavdlFdctpVQLQWLLDAgLGSDPAYQPAQ------VLIGGEAISPAVWSRLQS 751
Cdd:PRK05677  280 NILISNPRDLPAMvkelGKWKFSG------F----VGLNTLFVA-LCNNEAFRKLDfsalklTLSGGMALQLATAERWKE 348

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  752 LSDTRFINVYGPTECTVDATACVVDRTQpLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAE 831
Cdd:PRK05677  349 VTGCAICEGYGMTETSPVVSVNPSQAIQ-VGTIGIPVPSTLCKVIDDDGNELPLGEVGELCVKGPQVMKGYWQRPEATDE 427

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  832 RFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLV 911
Cdd:PRK05677  428 ILDSDGW--------LKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGEAIK 499

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967  912 AYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK05677  500 VFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRREL 551
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1552-2021 3.26e-30

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 127.19  E-value: 3.26e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1552 QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLt 1631
Cdd:cd05910     1 SRLSFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKNLKQCLQEAEPDAFI- 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1632 qanqralltgDVPRilldtadfshlsednphvpgldAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDD 1711
Cdd:cd05910    80 ----------GIPK----------------------ADEPAAILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGE 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1712 RVLQKTPfsfdvsVWEFFWPLLYGARLVMA----RPdGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADA-DCACDS 1786
Cdd:cd05910   128 VDLATFP------LFALFGPALGLTSVIPDmdptRP-ARADPQKLVGAIRQYGVSIVFGSPALLERVARYCAQhGITLPS 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1787 LRRVICSGEALPAELQQRF--FARFNAQLHNLYGPTEA----AID----VTFWACQPDDHRSfVPIGRPIANTQLYILDT 1856
Cdd:cd05910   201 LRRVLSAGAPVPIALAARLrkMLSDEAEILTPYGATEAlpvsSIGsrelLATTTAATSGGAG-TCVGRPIPGVRVRIIEI 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1857 LGQP---------VPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPfinqPGARLYKTGDLARWLPDGSLEYLGRNDF 1927
Cdd:cd05910   280 DDEPiaewddtleLPRGEIGEITVTGPTVTPTYVNRPVATALAKIDDN----SEGFWHRMGDLGYLDDEGRLWFCGRKAH 355

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1928 QVKLRGFRIELGEIEARLMQCPGVQEAVVVAReDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVP---GAFVT 2004
Cdd:cd05910   356 RVITTGGTLYTEPVERVFNTHPGVRRSALVGV-GKPGCQLPVLCVEPLPGTITPRARLEQELRALAKDYPHTqriGRFLI 434

                         490
                  ....*....|....*....
gi 641744967 2005 LDAFPLTP--NGKLDRKAL 2021
Cdd:cd05910   435 HPSFPVDIrhNAKIFREKL 453
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
 1549-2018 3.39e-30

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 127.17  E-value: 3.39e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1549 FEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVA 1628
Cdd:cd05914     3 YGGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKA 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1629 LLTQANqralltgdvprilldtadfshlsEDnphvpgldahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLT 1708
Cdd:cd05914    83 IFVSDE-----------------------DD-----------VALINYTSGTTGNSKGVMLTYRNIVSNVDGVKEVVLLG 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1709 PDDRVLQKTPFSFDVS-VWEFFWPLLYGARLV-MARPDGHK-DAAYLAQLIERTGITTLHFV----------PSMLQQFV 1775
Cdd:cd05914   129 KGDKILSILPLHHIYPlTFTLLLPLLNGAHVVfLDKIPSAKiIALAFAQVTPTLGVPVPLVIekifkmdiipKLTLKKFK 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1776 QWADADCACDSLR---------------RVICSGEA-LPAELQQrFFARFNAQLHNLYGPTEAAIDVTFwacQPDDHRSF 1839
Cdd:cd05914   209 FKLAKKINNRKIRklafkkvheafggniKEFVIGGAkINPDVEE-FLRTIGFPYTIGYGMTETAPIISY---SPPNRIRL 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1840 VPIGRPIANTQLYILDtlgqPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSL 1919
Cdd:cd05914   285 GSAGKVIDGVEVRIDS----PDPATGEGEIIVRGPNVMKGYYKNPEATAEAFDKDGW--------FHTGDLGKIDAEGYL 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1920 EYLGR-NDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDspgdtRLVAYlcpqpgVTPDPADL------------- 1985
Cdd:cd05914   353 YIRGRkKEMIVLSSGKNIYPEEIEAKINNMPFVLESLVVVQEK-----KLVAL------AYIDPDFLdvkalkqrniida 421

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967 1986 -----RQQLGQHLAEY-MVPGAFVTLDAFPLTPNGKLDR 2018
Cdd:cd05914   422 ikwevRDKVNQKVPNYkKISKVKIVKEEFEKTPKGKIKR 460
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
 1530-2026 3.81e-30

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 129.00  E-value: 3.81e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLD 1609
Cdd:PRK06710   26 LHKYVEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTN 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1610 PGYPAERLAYMLDDASPVALL--------------TQANQRALLTG-----DVPRILL---------------DTADFSH 1655
Cdd:PRK06710  106 PLYTERELEYQLHDSGAKVILcldlvfprvtnvqsATKIEHVIVTRiadflPFPKNLLypfvqkkqsnlvvkvSESETIH 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1656 L-----SEDNP--HVPGLDAHHLAYVIYTSGSTGKPKGVMNSHRALC-NRLVWMQNTYRLTPDDRVLqktpfsfdVSVWE 1727
Cdd:PRK06710  186 LwnsveKEVNTgvEVPCDPENDLALLQYTGGTTGFPKGVMLTHKNLVsNTLMGVQWLYNCKEGEEVV--------LGVLP 257

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1728 FFWplLYGARLVM--ARPDGHK-------DAAYLAQLIERTGITTLHFVPSMLQQFVQ---WADADCAcdSLRRVICSGE 1795
Cdd:PRK06710  258 FFH--VYGMTAVMnlSIMQGYKmvlipkfDMKMVFEAIKKHKVTLFPGAPTIYIALLNsplLKEYDIS--SIRACISGSA 333

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1796 ALPAELQQRFFARFNAQLHNLYGPTEAAidvtfwacqPDDHRSF-----VP--IGRPIANTQLYILD-TLGQPVPLGVAG 1867
Cdd:PRK06710  334 PLPVEVQEKFETVTGGKLVEGYGLTESS---------PVTHSNFlwekrVPgsIGVPWPDTEAMIMSlETGEALPPGEIG 404

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1868 ELHIGGVGVARGYLNRPDLTAErFIPDPFINqpgarlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQ 1947
Cdd:PRK06710  405 EIVVKGPQIMKGYWNKPEETAA-VLQDGWLH--------TGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYE 475

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 1948 CPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPAPDQ 2026
Cdd:PRK06710  476 HEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIEEEK 554
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
 1538-2023 4.24e-30

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 128.19  E-value: 4.24e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERL 1617
Cdd:PRK13383   45 AARWPGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDAL 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDdASPVALLTQANQ---RALLTGDVPRILLDTADFSHLSEDNPHV--PGldahhlAYVIYTSGSTGKPKGVMNSHR 1692
Cdd:PRK13383  125 AAALR-AHHISTVVADNEfaeRIAGADDAVAVIDPATAGAEESGGRPAVaaPG------RIVLLTSGTTGKPKGVPRAPQ 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1693 ALCNRLVWMQ--NTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARpdgHKDA-AYLAQliertgiTTLH---- 1765
Cdd:PRK13383  198 LRSAVGVWVTilDRTRLRTGSRISVAMPMFHGLGLGMLMLTIALGGTVLTHR---HFDAeAALAQ-------ASLHrada 267

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1766 --FVPSMLQQFVQWAD---ADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTfwaCQPDDHRSFV 1840
Cdd:PRK13383  268 ftAVPVVLARILELPPrvrARNPLPQLRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGSTEVGIGAL---ATPADLRDAP 344

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1841 -PIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNrpdlTAERFIPDPfinqpgarLYKTGDLARWLPDGSL 1919
Cdd:PRK13383  345 eTVGKPVAGCPVRILDRNNRPVGPRVTGRIFVGGELAGTRYTD----GGGKAVVDG--------MTSTGDMGYLDNAGRL 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1920 EYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVP 1999
Cdd:PRK13383  413 FIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSGVDAAQLRDYLKDRVSRFEQP 492

                         490       500
                  ....*....|....*....|....
gi 641744967 2000 GAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK13383  493 RDINIVSSIPRNPTGKVLRKELPG 516
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
 483-965 4.43e-30

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 127.81  E-value: 4.43e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:PRK13383   45 AARWPGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDAL 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTN--PVVqglhAAHLAYVIYTSGSTGRPKGVMVAH--RN 638
Cdd:PRK13383  125 AAALRAHHISTVVADNEFAERIAGADDAVAVIDPATAGAEESGgrPAV----AAPGRIVLLTSGTTGKPKGVPRAPqlRS 200

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  639 VINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQLLSGHTLVLVPDALraDAHQLWRYFARHAVDLFDCTPVQLQWL 718
Cdd:PRK13383  201 AVGVWVTILDRTRLRTGSRISVAMPMFHGLGLGMLMLTIALGGTVLTHRHF--DAEAALAQASLHRADAFTAVPVVLARI 278

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  719 LDAG---LGSDPAYQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYI 795
Cdd:PRK13383  279 LELPprvRARNPLPQLRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGSTEVGIGALATPADLRDAPETVGKPVAGCPVRI 358

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  796 LDAQDQPVPIGVTGELHIGGAGVARGYLHrpdlTAERFIPDPFSAdpaariykTGDLARWLPDGNIDYLGRNDFQIKVRG 875
Cdd:PRK13383  359 LDRNNRPVGPRVTGRIFVGGELAGTRYTD----GGGKAVVDGMTS--------TGDMGYLDNAGRLFIVGREDDMIISGG 426

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  876 FRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPN 955
Cdd:PRK13383  427 ENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSGVDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPT 506

                         490
                  ....*....|
gi 641744967  956 GKLDRKALPA 965
Cdd:PRK13383  507 GKVLRKELPG 516
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
 1533-2015 5.79e-30

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 127.03  E-value: 5.79e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1533 LVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGY 1612
Cdd:cd12118     9 FLERAAAVYPDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRL 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1613 PAERLAYMLDDASPVALL--TQANQRALLTGDVPrilldtadfshlseDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNS 1690
Cdd:cd12118    89 DAEEIAFILRHSEAKVLFvdREFEYEDLLAEGDP--------------DFEWIPPADEWDPIALNYTSGTTGRPKGVVYH 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1691 HR-----ALCNRLVWmqntyRLTPDDRVLQKTPFsFDVSVWEFFW-PLLYGARLVMARpdgHKDAAYLAQLIERTGITtl 1764
Cdd:cd12118   155 HRgaylnALANILEW-----EMKQHPVYLWTLPM-FHCNGWCFPWtVAAVGGTNVCLR---KVDAKAIYDLIEKHKVT-- 223

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1765 HF--VPSMLQQFVQWADADCACDSLR-RVICSGEALPAELQQRFfARFNAQLHNLYGPTEAAIDVTFWACQPD-DH---- 1836
Cdd:cd12118   224 HFcgAPTVLNMLANAPPSDARPLPHRvHVMTAGAPPPAAVLAKM-EELGFDVTHVYGLTETYGPATVCAWKPEwDElpte 302

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1837 -------RSFVPIgrpIANTQLYILDT-LGQPVPL-GV-AGELHIGGVGVARGYLNRPDLTAERFipdpfinQPGarLYK 1906
Cdd:cd12118   303 erarlkaRQGVRY---VGLEEVDVLDPeTMKPVPRdGKtIGEIVFRGNIVMKGYLKNPEATAEAF-------RGG--WFH 370

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1907 TGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLR 1986
Cdd:cd12118   371 SGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEEEII 450

                         490       500
                  ....*....|....*....|....*....
gi 641744967 1987 QQLGQHLAEYMVPGAFVTLDaFPLTPNGK 2015
Cdd:cd12118   451 AFCREHLAGFMVPKTVVFGE-LPKTSTGK 478
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
 499-965 7.91e-30

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 128.38  E-value: 7.91e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:cd05968    92 LTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAEAKALITAD 171

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 AQVAQLNSTLPTVLLDTpAAAACPDTNPVV-------------------------QGLHAAHLA-----YVIYTSGSTGR 628
Cdd:cd05968   172 GFTRRGREVNLKEEADK-ACAQCPTVEKVVvvrhlgndftpakgrdlsydeeketAGDGAERTEsedplMIIYTSGTTGK 250

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  629 PKGVMVAHrnvinlatglhtllaLDHPSRIALNASIVFDasVKN-----WIQ--------------LLSGHTLVL---VP 686
Cdd:cd05968   251 PKGTVHVH---------------AGFPLKAAQDMYFQFD--LKPgdlltWFTdlgwmmgpwlifggLILGATMVLydgAP 313

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  687 DALRADahQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQV-LIG--GEAISPAVWSRL-QSLSDTR--FINV 760
Cdd:cd05968   314 DHPKAD--RLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLrVLGstGEPWNPEPWNWLfETVGKGRnpIINY 391

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  761 YGPTECTVDATACVVDRtqPLPTIG--KPLANTRLYILDAQDQPVPiGVTGELHIGGA--GVARGYLHRPDltaeRFIPD 836
Cdd:cd05968   392 SGGTEISGGILGNVLIK--PIKPSSfnGPVPGMKADVLDESGKPAR-PEVGELVLLAPwpGMTRGFWRDED----RYLET 464

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  837 PFSADPAARIYktGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCA 916
Cdd:cd05968   465 YWSRFDNVWVH--GDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVL 542

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|...
gi 641744967  917 RPDAELHPaALRQQLAASLADYM----IPSAFVTLDALPLTPNGKLDRKALPA 965
Cdd:cd05968   543 KPGVTPTE-ALAEELMERVADELgkplSPERILFVKDLPKTRNAKVMRRVIRA 594
PRK06188 PRK06188
acyl-CoA synthetase; Validated
 2620-3100 7.98e-30

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 127.41  E-value: 7.98e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAI----CVERGLDMVVGLLgilkAGGAYVPLDPTYPVERL 2695
Cdd:PRK06188   26 PDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALlslnRPEVLMAIGAAQL----AGLRRTALHPLGSLDDH 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2696 RYMLDDAKPVALISQSAH-------LGIMNGSLPVIL-LDDGETRP-----FDNEPDTPLDARKQgltPRHLAYVIYTSG 2762
Cdd:PRK06188  102 AYVLEDAGISTLIVDPAPfveralaLLARVPSLKHVLtLGPVPDGVdllaaAAKFGPAPLVAAAL---PPDIAGLAYTGG 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2763 STGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILeiFLP-LLNGARLILATQAQAADAQQlamLIER 2841
Cdd:PRK06188  179 TTGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHAGGAF--FLPtLLRGGTVIVLAKFDPAEVLR---AIEE 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2842 HAVSFMQATPSTWRMLVE---LRDFALPPGFKALCGGEAL-PENLATALLQKVTTLWNLYGPTET--TIwSTL----NGL 2911
Cdd:PRK06188  254 QRITATFLVPTMIYALLDhpdLRTRDLSSLETVYYGASPMsPVRLAEAIERFGPIFAQYYGQTEApmVI-TYLrkrdHDP 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2912 TTPTPYI--GHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDpfsgapearMYKTGDLGRW 2989
Cdd:PRK06188  333 DDPKRLTscGRPTPGLRVALLDEDGREVAQGEVGEICVRGPLVMDGYWNRPEETAEAFRDG---------WLHTGDVARE 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2990 LPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGV 3069
Cdd:PRK06188  404 DEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAAELQAHVKERK 483

                         490       500       510
                  ....*....|....*....|....*....|.
gi 641744967 3070 AEYMIPSAFVTLDAFPLTPNGKLDRKALPAP 3100
Cdd:PRK06188  484 GSVHAPKQVDFVDSLPLTALGKPDKKALRAR 514
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
 2628-3033 8.06e-30

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 126.32  E-value: 8.06e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2628 GEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVAL 2707
Cdd:cd17640     2 PPKRITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVAL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2708 IsqsahlgimngslpvillddgetrpFDNEPDTpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHAN----MVNFLCS 2783
Cdd:cd17640    82 V-------------------------VENDSDD-------------LATIIYTSGTTGNPKGVMLTHANllhqIRSLSDI 123

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2784 MRKEPGiaqedvllgvtslsfDISILeiFLPLLNGA-RL----ILATQAQAADAQQLAML--IERHAVSFMQATPSTWRM 2856
Cdd:cd17640   124 VPPQPG---------------DRFLS--ILPIWHSYeRSaeyfIFACGCSQAYTSIRTLKddLKRVKPHYIVSVPRLWES 186

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2857 LVE---------------LRDFALPPG-FK-ALCGGEALPENLATALLQKVTTLWNLYGPTETTIWSTLNGLTTPT-PYI 2918
Cdd:cd17640   187 LYSgiqkqvsksspikqfLFLFFLSGGiFKfGISGGGALPPHVDTFFEAIGIEVLNGYGLTETSPVVSARRLKCNVrGSV 266

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2919 GHPIANTQIYILDAQGRVV-PLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEY 2997
Cdd:cd17640   267 GRPLPGTEIKIVDPEGNVVlPPGEKGIVWVRGPQVMKGYYKNPEATSKVLDSDGW--------FNTGDLGWLTCGGELVL 338

                         410       420       430
                  ....*....|....*....|....*....|....*..
gi 641744967 2998 LGR-NDFQVKVRGFRIELGEIETRLARCHGVHDAVVI 3033
Cdd:cd17640   339 TGRaKDTIVLSNGENVEPQPIEEALMRSPFIEQIMVV 375
PRK08633 PRK08633
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
 1664-2021 9.17e-30

2-acyl-glycerophospho-ethanolamine acyltransferase; Validated


Pssm-ID: 236315 [Multi-domain]  Cd Length: 1146  Bit Score: 130.43  E-value: 9.17e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1664 PGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPF--SFDVSVWEFFwPLLYGARLVmA 1741
Cdd:PRK08633  777 PTFKPDDTATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFfhSFGLTVTLWL-PLLEGIKVV-Y 854

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1742 RPDGhKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCAC-DSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPT 1820
Cdd:PRK08633  855 HPDP-TDALGIAKLVAKHRATILLGTPTFLRLYLRNKKLHPLMfASLRLVVAGAEKLKPEVADAFEEKFGIRILEGYGAT 933

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1821 E----AAI------DVTFWAcQPDDHRSFVpiGRPIANTQLYILD-TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAE 1889
Cdd:PRK08633  934 EtspvASVnlpdvlAADFKR-QTGSKEGSV--GMPLPGVAVRIVDpETFEELPPGEDGLILIGGPQVMKGYLGDPEKTAE 1010

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1890 rFIPDPfinqPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQ--CPGVQEAVVVAREDSPGDTR 1967
Cdd:PRK08633 1011 -VIKDI----DGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEEELAKalGGEEVVFAVTAVPDEKKGEK 1085

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1968 LVAYLCPQPGvtpDPADLRQQLGQ-HLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK08633 1086 LVVLHTCGAE---DVEELKRAIKEsGLPNLWKPSRYFKVEALPLLGSGKLDLKGL 1137
starter-C_NRPS cd19533
Starter Condensation domains, found in the first module of nonribosomal peptide synthetases ...
 2162-2500 1.01e-29

Starter Condensation domains, found in the first module of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. While standard C-domains catalyze peptide bond formation between two amino acids, an initial, ('starter') C-domain may instead acylate an amino acid with a fatty acid. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380456 [Multi-domain]  Cd Length: 419  Bit Score: 125.17  E-value: 1.01e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2162 YPLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDLsQPVQVVWRQAILPINH 2241
Cdd:cd19533     2 LPLTSAQRGVWFAEQLDPEGSIYNLAEYLEITGPVDLAVLERALRQVIAEAETLRLRFTEEEG-EPYQWIDPYTPVPIRH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2242 FEPTSPEDVLAQLQAHTEPRTRR-IDLSQAPLFRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADAL 2320
Cdd:cd19533    81 IDLSGDPDPEGAAQQWMQEDLRKpLPLDNDPLFRHALFTLG-DNRHFWYQRVHHIVMDGFSFALFGQRVAEIYTALLKGR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2321 PTP----LPYRNFIAQTLSVPNSAH----EAYFRDKLADVDEPTapfgLLNVQGSGG--DIHEARLVLDATLASAIRQQA 2390
Cdd:cd19533   160 PAPpapfGSFLDLVEEEQAYRQSERferdRAFWTEQFEDLPEPV----SLARRAPGRslAFLRRTAELPPELTRTLLEAA 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2391 RHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRLAGAEgadRIM-GMFINTLPLRISLA-DRGAAEVVERTSHDLM 2468
Cdd:cd19533   236 EAHGASWPSFFIALVAAYLHRLTGANDVVLGVPVMGRLGAAA---RQTpGMVANTLPLRLTVDpQQTFAELVAQVSRELR 312

                         330       340       350
                  ....*....|....*....|....*....|...
gi 641744967 2469 TLLEHEQAPLALAQRCSGVAPPM-PLFSTLLNY 2500
Cdd:cd19533   313 SLLRHQRYRYEDLRRDLGLTGELhPLFGPTVNY 345
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
 496-966 1.18e-29

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 126.35  E-value: 1.18e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  496 DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALL 575
Cdd:PRK12406    9 DRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLI 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  576 TQSAQVAQLNSTLP---TVL-LDTPA--------AAACPDTNPVVQGLH---AAHLAY----------VIYTSGSTGRPK 630
Cdd:PRK12406   89 AHADLLHGLASALPagvTVLsVPTPPeiaaayriSPALLTPPAGAIDWEgwlAQQEPYdgppvpqpqsMIYTSGTTGHPK 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  631 GV---------------MVAHrnVINLATGLHTLLA--LDH--PSRIALNASIVfdasvknwiqllsGHTLVLVPdalRA 691
Cdd:PRK12406  169 GVrraaptpeqaaaaeqMRAL--IYGLKPGIRALLTgpLYHsaPNAYGLRAGRL-------------GGVLVLQP---RF 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  692 DAHQLWRYFARHAVDLFDCTPVQLQWLLDAglgsdpayqPAQV-----------LIGGEAISPA-VWSRLQSLSDTRFIN 759
Cdd:PRK12406  231 DPEELLQLIERHRITHMHMVPTMFIRLLKL---------PEEVrakydvsslrhVIHAAAPCPAdVKRAMIEWWGPVIYE 301

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  760 VYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVargylhrPDLTaerfipdpFS 839
Cdd:PRK12406  302 YYGSTESGAVTFATSEDALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGN-------PDFT--------YH 366

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  840 ADPAARiyKTGDLARWLPDGNIDYLG----------RNDFQIKvRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTR 909
Cdd:PRK12406  367 NKPEKR--AEIDRGGFITSGDVGYLDadgylflcdrKRDMVIS-GGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEA 443

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967  910 LVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAP 966
Cdd:PRK12406  444 LMAVVEPQPGATLDEADIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDP 500
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
 2754-3097 1.21e-29

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 122.44  E-value: 1.21e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2754 LAYVIYTSGSTGKPKGVMVEHANMV-NFLCSMRKEPGIAQEDVLLgvtSL-SFDISILEI-FLPLLNGARLILATQAQAA 2830
Cdd:cd17630     2 LATVILTSGSTGTPKAVVHTAANLLaSAAGLHSRLGFGGGDSWLL---SLpLYHVGGLAIlVRSLLAGAELVLLERNQAL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2831 DAQqlamlIERHAVSFMQATPST-WRMLVELRDFALPPGFKA-LCGGEALPENLATALLQKVTTLWNLYGPTETTiwSTL 2908
Cdd:cd17630    79 AED-----LAPPGVTHVSLVPTQlQRLLDSGQGPAALKSLRAvLLGGAPIPPELLERAADRGIPLYTTYGMTETA--SQV 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 NGLTTPTP---YIGHPIANTQIYILDAqgrvvplgvaGEIHIAGAGVVRGYLGRPdltaerfITDPFsgaPEARMYKTGD 2985
Cdd:cd17630   152 ATKRPDGFgrgGVGVLLPGRELRIVED----------GEIWVGGASLAMGYLRGQ-------LVPEF---NEDGWFTTKD 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2986 LGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAylLAQPDTVLEPADLRQRL 3065
Cdd:cd17630   212 LGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVA--VIVGRGPADPAELRAWL 289

                         330       340       350
                  ....*....|....*....|....*....|..
gi 641744967 3066 SEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd17630   290 KDKLARFKLPKRIYPVPELPRTGGGKVDRRAL 321
beta-lac_NRPS cd19547
Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis ...
 1069-1481 1.34e-29

Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis NocB which exhibits an unusual cyclization to form beta-lactam rings in pro-nocardicin G synthesis; Nocardia uniformis NRPS NocB acts centrally in the biosynthesis of the nocardicin monocyclic beta-lactam antibiotics. Along with another NRPS NocA, it mediates an unusual cyclization to form beta-lactam rings in the synthesis of the beta-lactam-containing pentapeptide pro-nocardicin G. This small subfamily is related to DCL-type Condensation (C) domains, which catalyze condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; domains belonging to this subfamily have an HHHxxxD motif at the active site.


Pssm-ID: 380469 [Multi-domain]  Cd Length: 422  Bit Score: 124.73  E-value: 1.34e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1069 PLSFSQQRLWFLAQLDPAaSQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSID-GQPAQQIDPD------T 1141
Cdd:cd19547     3 PLAPMQEGMLFRGLFWPD-SDAYFNQNVLELVGGTDEDVLREAWRRVADRYEILRTGFTWRDrAEPLQYVRDDlappwaL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1142 LGFSLSSHDlrkldeaarttRVAELAEQ----EARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARE 1217
Cdd:cd19547    82 LDWSGEDPD-----------RRAELLERlladDRAAGLSLADCPLYRLTLVRLGGGRHYLLWSHHHILLDGWCLSLIWGD 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1218 LAALYQAALEGSEANLPPLPvQYADYAVWQR-QWLQGEtlnDLRDYWRDQLQG-APA-LLEIPTDRprpsvqryAGD-QV 1293
Cdd:cd19547   151 VFRVYEELAHGREPQLSPCR-PYRDYVRWIRaRTAQSE---ESERFWREYLRDlTPSpFSTAPADR--------EGEfDT 218

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1294 PFHLDAGQLRRLHA-LNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVANRPrQELEG---MVGFFVNTLALRTEPG 1369
Cdd:cd19547   219 VVHEFPEQLTRLVNeAARGYGVTTNAISQAAWSMLLALQTGARDVVHGLTIAGRP-PELEGsehMVGIFINTIPLRIRLD 297

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1370 RCHAVADLLDQVRERALDAYAHQALPFEQVVEILQPARsLSYSPIFQVMLSLNNTPAQALTLPDLTLSAVE-RPQHSTHF 1448
Cdd:cd19547   298 PDQTVTGLLETIHRDLATTAAHGHVPLAQIKSWASGER-LSGGRVFDNLVAFENYPEDNLPGDDLSIQIIDlHAQEKTEY 376

                         410       420       430
                  ....*....|....*....|....*....|...
gi 641744967 1449 DLSLSLIETENgLNGGLVYATDLFDRETILRVV 1481
Cdd:cd19547   377 PIGLIVLPLQK-LAFHFNYDTTHFTRAQVDRFI 408
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
 1670-2018 2.66e-29

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 121.36  E-value: 2.66e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1670 HLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMarpDGHKDA 1749
Cdd:cd17633     1 NPFYIGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSHSLFLYGAISALYLGGTFIG---QRKFNP 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1750 AYLAQLIERTGITTLHFVPSMLQQFVQwadADCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIdVTF 1828
Cdd:cd17633    78 KSWIRKINQYNATVIYLVPTMLQALAR---TLEPESKIKSIFSSGQKLFESTKKKLKNIFpKANLIEFYGTSELSF-ITY 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1829 WAcqPDDHRSFVPIGRPIANTQLYILDTLGqpvplGVAGELHIGGVGVARGYLNRPDLTAERFipdpfinqpgarlYKTG 1908
Cdd:cd17633   154 NF--NQESRPPNSVGRPFPNVEIEIRNADG-----GEIGKIFVKSEMVFSGYVRGGFSNPDGW-------------MSVG 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1909 DLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLcpqPGVTPDPADLRQQ 1988
Cdd:cd17633   214 DIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALY---SGDKLTYKQLKRF 290

                         330       340       350
                  ....*....|....*....|....*....|
gi 641744967 1989 LGQHLAEYMVPGAFVTLDAFPLTPNGKLDR 2018
Cdd:cd17633   291 LKQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
 2622-3097 8.17e-29

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 122.59  E-value: 8.17e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2622 AIAVVFGEASLSYDELNRRANRLAHHLIS-FGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLD 2700
Cdd:cd05958     1 RTCLRSPEREWTYRDLLALANRIANVLVGeLGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2701 DAKPValisqsahlgimngslpVILLDDGETRPFDnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMV-N 2779
Cdd:cd05958    81 KARIT-----------------VALCAHALTASDD------------------ICILAFTSGTTGAPKATMHFHRDPLaS 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRKEPGIAQEDVLLGVTSLSFDISI-LEIFLPLLNGARLILATQAQAADAQQlamLIERHAVSFMQATPSTWRMLV 2858
Cdd:cd05958   126 ADRYAVNVLRLREDDRFVGSPPLAFTFGLgGVLLFPFGVGASGVLLEEATPDLLLS---AIARYKPTVLFTAPTAYRAML 202

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2859 ELRDFA---LPPGFKALCGGEALPENLATALLQKV-TTLWNLYGPTET--TIWSTLNGLTTPTPyIGHPIANTQIYILDA 2932
Cdd:cd05958   203 AHPDAAgpdLSSLRKCVSAGEALPAALHRAWKEATgIPIIDGIGSTEMfhIFISARPGDARPGA-TGKPVPGYEAKVVDD 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2933 QGRVVPlgvAGEIhiaGAGVVRGylgrPdlTAERFITDPfsgapEARMY------KTGDLGRWLPDGTLEYLGRNDFQVK 3006
Cdd:cd05958   282 EGNPVP---DGTI---GRLAVRG----P--TGCRYLADK-----RQRTYvqggwnITGDTYSRDPDGYFRHQGRSDDMIV 344

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3007 VRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPA---DLRQRLSEGVAEYMIPSAFVTLDA 3083
Cdd:cd05958   345 SGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVlarELQDHAKAHIAPYKYPRAIEFVTE 424

                         490
                  ....*....|....
gi 641744967 3084 FPLTPNGKLDRKAL 3097
Cdd:cd05958   425 LPRTATGKLQRFAL 438
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
 496-963 8.96e-29

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 124.36  E-value: 8.96e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  496 DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALL 575
Cdd:PRK07059   46 GKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIV 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  576 TqsaqVAQLNSTLPTVLLDTPA----AAACPD--------TNPVV---------------------------QGLHAAHL 616
Cdd:PRK07059  126 V----LENFATTVQQVLAKTAVkhvvVASMGDllgfkghiVNFVVrrvkkmvpawslpghvrfndalaegarQTFKPVKL 201

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  617 -----AYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLL--ALDHPSRI-------ALNASIVFDASVKNWIQLLSGHTL 682
Cdd:PRK07059  202 gpddvAFLQYTGGTTGVSKGATLLHRNIVANVLQMEAWLqpAFEKKPRPdqlnfvcALPLYHIFALTVCGLLGMRTGGRN 281

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  683 VLVPDALraDAHQLWRYFARHAVDLFdctPVqLQWLLDAgLGSDPAYQP---AQVLI---GGEAISPAVWSRLQSLSDTR 756
Cdd:PRK07059  282 ILIPNPR--DIPGFIKELKKYQVHIF---PA-VNTLYNA-LLNNPDFDKldfSKLIVangGGMAVQRPVAERWLEMTGCP 354

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  757 FINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPD 836
Cdd:PRK07059  355 ITEGYGLSETSPVATCNPVDATEFSGTIGLPLPSTEVSIRDDDGNDLPLGEPGEICIRGPQVMAGYWNRPDETAKVMTAD 434

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  837 PFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDS-PGDTrlVAYLC 915
Cdd:PRK07059  435 GF--------FRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLEVAAVGVPDEhSGEA--VKLFV 504

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 641744967  916 ARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK07059  505 VKKDPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRREL 552
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
 1543-2023 1.03e-28

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 124.62  E-value: 1.03e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1543 DTTAVLFEDQH----LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLA 1618
Cdd:PRK04319   59 DKVALRYLDASrkekYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVR 138

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1619 YMLDDASPVALLTQAN-------------QRALLTGDVPRILLDTADFSHLSE---DNPHVPGLDAHHLAYVIYTSGSTG 1682
Cdd:PRK04319  139 DRLEDSEAKVLITTPAllerkpaddlpslKHVLLVGEDVEEGPGTLDFNALMEqasDEFDIEWTDREDGAILHYTSGSTG 218

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1683 KPKGVMNSHRALCNRLV---WMQNtyrLTPDDR---------VlqkTPFSFDVsvwefFWPLLYGARLVMARPDGHKDAA 1750
Cdd:PRK04319  219 KPKGVLHVHNAMLQHYQtgkYVLD---LHEDDVywctadpgwV---TGTSYGI-----FAPWLNGATNVIDGGRFSPERW 287

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1751 YlaQLIERTGITTLHFVPS---MLQQfvqwADADCA----CDSLRRVICSGEALPAE--------LQQRFfarfnaqlHN 1815
Cdd:PRK04319  288 Y--RILEDYKVTVWYTAPTairMLMG----AGDDLVkkydLSSLRHILSVGEPLNPEvvrwgmkvFGLPI--------HD 353

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1816 LYGPTE-AAIDVTFWACQPDDHRSfvpIGRPIANTQLYILDTLGQPVPLGVAGELHI--GGVGVARGYLNRPDLTAERFI 1892
Cdd:PRK04319  354 NWWMTEtGGIMIANYPAMDIKPGS---MGKPLPGIEAAIVDDQGNELPPNRMGNLAIkkGWPSMMRGIWNNPEKYESYFA 430

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1893 PDpfinqpgarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYL 1972
Cdd:PRK04319  431 GD---------WYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFV 501

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1973 CPQPGVTPDPAdLRQQLGQH----LAEYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK04319  502 ALRPGYEPSEE-LKEEIRGFvkkgLGAHAAPREIEFKDKLPKTRSGKIMRRVLKA 555
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 621-960 1.08e-28

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 120.46  E-value: 1.08e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  621 YTSGSTGRPKGVMVAHRNVINLA--TGLHTLLALDHpsRIALNASI--VFDASVKNWIQLLSGHTLVLVPDALRADAhql 696
Cdd:cd05917     9 FTSGTTGSPKGATLTHHNIVNNGyfIGERLGLTEQD--RLCIPVPLfhCFGSVLGVLACLTHGATMVFPSPSFDPLA--- 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  697 wryfARHAVDLFDCT-----PVQLQWLLDagLGSDPAYQPAQV---LIGGEAISPAVWSRLQS-LSDTRFINVYGPTECT 767
Cdd:cd05917    84 ----VLEAIEKEKCTalhgvPTMFIAELE--HPDFDKFDLSSLrtgIMAGAPCPPELMKRVIEvMNMKDVTIAYGMTETS 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  768 VDATACVVDRTQP--LPTIGKPLANTRLYILDAQDQPVP-IGVTGELHIGGAGVARGYLHRPDLTAERFIPDpfsadpaa 844
Cdd:cd05917   158 PVSTQTRTDDSIEkrVNTVGRIMPHTEAKIVDPEGGIVPpVGVPGELCIRGYSVMKGYWNDPEKTAEAIDGD-------- 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  845 RIYKTGDLARWLPDGNIDYLGRndfqIK---VRGFR-IEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDA 920
Cdd:cd05917   230 GWLHTGDLAVMDEDGYCRIVGR----IKdmiIRGGEnIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEGA 305

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|
gi 641744967  921 ELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDR 960
Cdd:cd05917   306 ELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQK 345
C_PKS-NRPS cd19532
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 64-405 1.78e-28

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHxxxD motif, a few such as Monascus pilosus lovastatin nonaketide synthase MokA have a non-canonical HRxxxD motif in the C-domain and are unable to catalyze amide-bond formation. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380455 [Multi-domain]  Cd Length: 421  Bit Score: 121.41  E-value: 1.78e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   64 RLDAFLDVLQQVIARHDILRTAICWQ-GLHQPVQVVWRQAPLTVNTLTTTSSDTVPAQLRAAtdpSNHRLNLSNAPLLSA 142
Cdd:cd19532    37 DVARLERAVRAVGQRHEALRTCFFTDpEDGEPMQGVLASSPLRLEHVQISDEAEVEEEFERL---KNHVYDLESGETMRI 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  143 TtahdpvcgewLLSLS---------IHHLISDHITQALIIDEIRLLLEDRPEaLPKPLPYRNFIAQ----ILSVPLSEHE 209
Cdd:cd19532   114 V----------LLSLSptehylifgYHHIAMDGVSFQIFLRDLERAYNGQPL-LPPPLQYLDFAARqrqdYESGALDEDL 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  210 QYFRNRLADIDTPTAPFDLVDVQG----NGEDITEARLSLDSSLADALRRQARHLGISSsvlFHV---AWAQVLALTSGR 282
Cdd:cd19532   183 AYWKSEFSTLPEPLPLLPFAKVKSrpplTRYDTHTAERRLDAALAARIKEASRKLRVTP---FHFylaALQVLLARLLDV 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  283 DDVVFGSVLSGRlqGNLGADRVMGMFINTLPLRVSL-RERSVHDVVQATSHELMMLLAHEQAPL-ALAQQCsQVP---PP 357
Cdd:cd19532   260 DDICIGIADANR--TDEDFMETIGFFLNLLPLRFRRdPSQTFADVLKETRDKAYAALAHSRVPFdVLLDEL-GVPrsaTH 336

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967  358 LPLFSTLFNYRhsQKDASSQFWEGMrQLSGRE----RTNYPITLSVDDLGDG 405
Cdd:cd19532   337 SPLFQVFINYR--QGVAESRPFGDC-ELEGEEfedaRTPYDLSLDIIDNPDG 385
PRK07529 PRK07529
AMP-binding domain protein; Validated
 1521-2021 2.01e-28

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 124.30  E-value: 2.01e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1521 EADFPHDalIHQLVEDQAARTPDTTAVLF--------EDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMV 1592
Cdd:PRK07529   20 ARDLPAS--TYELLSRAAARHPDAPALSFlldadpldRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETH 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1593 IGLLAILKAGAAyVPLDPGYPAERLAYMLDDASPVALLTQA-------------------NQRALLTGDVPRILLDTADF 1653
Cdd:PRK07529   98 FALWGGEAAGIA-NPINPLLEPEQIAELLRAAGAKVLVTLGpfpgtdiwqkvaevlaalpELRTVVEVDLARYLPGPKRL 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1654 S-HLSEDNPHVPGLD--------------------AHHLAYVIYTSGSTGKPKGVMNSHRALCNRlVWMQNTY-RLTPDD 1711
Cdd:PRK07529  177 AvPLIRRKAHARILDfdaelarqpgdrlfsgrpigPDDVAAYFHTGGTTGMPKLAQHTHGNEVAN-AWLGALLlGLGPGD 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1712 RVLQKTP-FSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLA---QLIERTGITTLHFVPSMLQQFVQWADADCACDSL 1787
Cdd:PRK07529  256 TVFCGLPlFHVNALLVTGLAPLARGAHVVLATPQGYRGPGVIAnfwKIVERYRINFLSGVPTVYAALLQVPVDGHDISSL 335

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1788 RRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVtfwACQP-DDHRSFVPIGRPIANTQLYI--LDTLG---QPV 1861
Cdd:PRK07529  336 RYALCGAAPLPVEVFRRFEAATGVRIVEGYGLTEATCVS---SVNPpDGERRIGSVGLRLPYQRVRVviLDDAGrylRDC 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1862 PLGVAGELHIGGVGVARGYLNrPDLTAERFIPDPFINqpgarlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEI 1941
Cdd:PRK07529  413 AVDEVGVLCIAGPNVFSGYLE-AAHNKGLWLEDGWLN--------TGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAI 483

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1942 EARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAE-YMVPGAFVTLDAFPLTPNGKLDRKA 2020
Cdd:PRK07529  484 EEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASATEAELLAFARDHIAErAAVPKHVRILDALPKTAVGKIFKPA 563


                  .
gi 641744967 2021 L 2021
Cdd:PRK07529  564 L 564
PRK07470 PRK07470
acyl-CoA synthetase; Validated
 483-963 2.23e-28

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 122.84  E-value: 2.23e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:PRK07470   17 ARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFRQTPDEV 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLTQSA---QVAQLNSTLPTVLLDTPAAAAcpDTNPVVQGLHAAHL--------------AYVIYTSGS 625
Cdd:PRK07470   97 AYLAEASGARAMICHADfpeHAAAVRAASPDLTHVVAIGGA--RAGLDYEALVARHLgarvanaavdhddpCWFFFTSGT 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  626 TGRPKGVMVAHRNVINLATGlHtlLALDHPSRIALNASIVF-----DASVKNWIQLLSGHTLVLVPdALRADAHQLWRYF 700
Cdd:PRK07470  175 TGRPKAAVLTHGQMAFVITN-H--LADLMPGTTEQDASLVVaplshGAGIHQLCQVARGAATVLLP-SERFDPAEVWALV 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  701 ARHAVDLFDCTPVQLQWLLDaglgsDPAYQPAQ------VLIGGEAISPAVWSR-LQSLSDTrFINVYGPTECTVDATAC 773
Cdd:PRK07470  251 ERHRVTNLFTVPTILKMLVE-----HPAVDRYDhsslryVIYAGAPMYRADQKRaLAKLGKV-LVQYFGLGEVTGNITVL 324

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  774 ------VVDRTQP-LPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaari 846
Cdd:PRK07470  325 ppalhdAEDGPDArIGTCGFERTGMEVQIQDDEGRELPPGETGEICVIGPAVFAGYYNNPEANAKAFRDGWF-------- 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  847 yKTGDLARWLPDGNIDYLGR-NDFQIKvRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPA 925
Cdd:PRK07470  397 -RTGDLGHLDARGFLYITGRaSDMYIS-GGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEA 474

                         490       500       510
                  ....*....|....*....|....*....|....*...
gi 641744967  926 ALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK07470  475 ELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMV 512
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
 2622-3092 2.38e-28

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 122.32  E-value: 2.38e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2622 AIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDD 2701
Cdd:PRK08276    2 AVIMAPSGEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDD 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2702 AKPVALISQSAHL-------GIMNGSLPVILLDDGETRPF-------DNEPDTPLDARKQGltprhlAYVIYTSGSTGKP 2767
Cdd:PRK08276   82 SGAKVLIVSAALAdtaaelaAELPAGVPLLLVVAGPVPGFrsyeealAAQPDTPIADETAG------ADMLYSSGTTGRP 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2768 KGVmvehanmvnflcsMRKEPGIAQEDVLLG-VTSLSFDISILE--IFL---PLLNGARLILATQAQAADAQQLAM---- 2837
Cdd:PRK08276  156 KGI-------------KRPLPGLDPDEAPGMmLALLGFGMYGGPdsVYLspaPLYHTAPLRFGMSALALGGTVVVMekfd 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2838 ------LIERHAVSFMQATPSTW-RMLvelrdfALPPGFK----------ALCGGEALPENLATALLQkvttlWnlYGPT 2900
Cdd:PRK08276  223 aeealaLIERYRVTHSQLVPTMFvRML------KLPEEVRarydvsslrvAIHAAAPCPVEVKRAMID-----W--WGPI 289

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2901 --ETTIWSTLNGLTTPTP--YIGHP-----IANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFItdp 2971
Cdd:PRK08276  290 ihEYYASSEGGGVTVITSedWLAHPgsvgkAVLGEVRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKTAAARN--- 366

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2972 fsgapEARMYKTGDLGrWL-PDGTLeYL-GRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAyll 3049
Cdd:PRK08276  367 -----PHGWVTVGDVG-YLdEDGYL-YLtDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKA--- 436

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 3050 aqpdtVLEPAD---------------LRQRLsegvAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:PRK08276  437 -----VVQPADgadagdalaaeliawLRGRL----AHYKCPRSIDFEDELPRTPTGKL 485
PRK08316 PRK08316
acyl-CoA synthetase; Validated
 2619-3092 2.41e-28

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 122.73  E-value: 2.41e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:PRK08316   24 YPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEELAYI 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISQSAHLGI------MNGSLPVILLD-------DGETRPFD----NEPDTPLDARkqgLTPRHLAYVIYTS 2761
Cdd:PRK08316  104 LDHSGARAFLVDPALAPTaeaalaLLPVDTLILSLvlggreaPGGWLDFAdwaeAGSVAEPDVE---LADDDLAQILYTS 180

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2762 GSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLsFDISILEIFL-P-LLNGARLILATQAQAADAQQlamLI 2839
Cdd:PRK08316  181 GTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIPLHALPL-YHCAQLDVFLgPyLYVGATNVILDAPDPELILR---TI 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2840 ERHAVSFMQATPSTWRMLVELRDFA---LPPGFKALCGGEALPENLATALLQKVTTL--WNLYGPTETTIWSTLNG---- 2910
Cdd:PRK08316  257 EAERITSFFAPPTVWISLLRHPDFDtrdLSSLRKGYYGASIMPVEVLKELRERLPGLrfYNCYGQTEIAPLATVLGpeeh 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2911 LTTPTPyIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmyKTGDLGRWL 2990
Cdd:PRK08316  337 LRRPGS-AGRPVLNVETRVVDDDGNDVAPGEVGEIVHRSPQLMLGYWDDPEKTAEAFRGGWF---------HSGDLGVMD 406

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2991 PDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADL----RQRLs 3066
Cdd:PRK08316  407 EEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTEDELiahcRARL- 485

                         490       500
                  ....*....|....*....|....*.
gi 641744967 3067 egvAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:PRK08316  486 ---AGFKVPKRVIFVDELPRNPSGKI 508
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
 494-921 3.45e-28

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 121.40  E-value: 3.45e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  494 FEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVA 573
Cdd:cd05914     3 YGGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKA 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  574 LLTqsaqvaqlnstlptvlldtpaaaacpdTNPvvqglhaAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALD 653
Cdd:cd05914    83 IFV---------------------------SDE-------DDVALINYTSGTTGNSKGVMLTYRNIVSNVDGVKEVVLLG 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  654 HPSRIA--LNASIVFDASVKNWIQLLSGHTLVLV--PDALRADAHQlwryFARHAVDLFDCTPVQLQ------------- 716
Cdd:cd05914   129 KGDKILsiLPLHHIYPLTFTLLLPLLNGAHVVFLdkIPSAKIIALA----FAQVTPTLGVPVPLVIEkifkmdiipkltl 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  717 ----WLLDAGLGSDPAYQPA-------------QVLIGGEAISPAVWSRLQSLSDTrFINVYGPTECTVDATACVVDRTQ 779
Cdd:cd05914   205 kkfkFKLAKKINNRKIRKLAfkkvheafggnikEFVIGGAKINPDVEEFLRTIGFP-YTIGYGMTETAPIISYSPPNRIR 283

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  780 pLPTIGKPLANTRLYIldaqDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDG 859
Cdd:cd05914   284 -LGSAGKVIDGVEVRI----DSPDPATGEGEIIVRGPNVMKGYYKNPEATAEAFDKDGW--------FHTGDLGKIDAEG 350

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967  860 NIDYLGR-NDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDspgdtRLVAYLCARPDAE 921
Cdd:cd05914   351 YLYIRGRkKEMIVLSSGKNIYPEEIEAKINNMPFVLESLVVVQEK-----KLVALAYIDPDFL 408
PRK07470 PRK07470
acyl-CoA synthetase; Validated
 2620-3097 3.84e-28

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 122.07  E-value: 3.84e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:PRK07470   21 PDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFRQTPDEVAYLA 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQSA---HLGIMNGSLP----VILLDDGE-TRPFDNEPDTPLDARKQGLTPRH--LAYVIYTSGSTGKPKG 2769
Cdd:PRK07470  101 EASGARAMICHADfpeHAAAVRAASPdlthVVAIGGARaGLDYEALVARHLGARVANAAVDHddPCWFFFTSGTTGRPKA 180

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2770 VMVEHANM----VNFLCSMRkePGIAQEDVLLGVTSLSFDISILEIfLPLLNGARLILATQAQAADAQQLAmLIERHAVS 2845
Cdd:PRK07470  181 AVLTHGQMafviTNHLADLM--PGTTEQDASLVVAPLSHGAGIHQL-CQVARGAATVLLPSERFDPAEVWA-LVERHRVT 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2846 FMQATPSTWRMLVE-----------LRD--FALPPGFKAlcggealPENLATALLQKVttLWNLYGPTETTiwstlNGLT 2912
Cdd:PRK07470  257 NLFTVPTILKMLVEhpavdrydhssLRYviYAGAPMYRA-------DQKRALAKLGKV--LVQYFGLGEVT-----GNIT 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2913 TPTPYI--------------GHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapea 2978
Cdd:PRK07470  323 VLPPALhdaedgpdarigtcGFERTGMEVQIQDDEGRELPPGETGEICVIGPAVFAGYYNNPEANAKAFRDGWF------ 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2979 rmyKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEP 3058
Cdd:PRK07470  397 ---RTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDE 473

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967 3059 ADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK07470  474 AELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMV 512
PRK08633 PRK08633
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
 2747-3097 8.31e-28

2-acyl-glycerophospho-ethanolamine acyltransferase; Validated


Pssm-ID: 236315 [Multi-domain]  Cd Length: 1146  Bit Score: 124.27  E-value: 8.31e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2747 QGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSL--SFDISIlEIFLPLLNGARLILA 2824
Cdd:PRK08633  777 PTFKPDDTATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFfhSFGLTV-TLWLPLLEGIKVVYH 855

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2825 TQAQAADAQQLamLIERHAVSFMQATPSTWRMLveLRDF-ALPPGFKAL----CGGEALPENLATALLQKV-TTLWNLYG 2898
Cdd:PRK08633  856 PDPTDALGIAK--LVAKHRATILLGTPTFLRLY--LRNKkLHPLMFASLrlvvAGAEKLKPEVADAFEEKFgIRILEGYG 931

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2899 PTETTIWSTLN------GLTTPTPY-----IGHPIANTQIYILDAQ-GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAEr 2966
Cdd:PRK08633  932 ATETSPVASVNlpdvlaADFKRQTGskegsVGMPLPGVAVRIVDPEtFEELPPGEDGLILIGGPQVMKGYLGDPEKTAE- 1010

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2967 FITDpfsgAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGvHDAVVIAREDSPGDKR--L 3044
Cdd:PRK08633 1011 VIKD----IDGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEEELAKALG-GEEVVFAVTAVPDEKKgeK 1085

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....
gi 641744967 3045 VAYLLAQPDTvlEPADLRQRLSE-GVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK08633 1086 LVVLHTCGAE--DVEELKRAIKEsGLPNLWKPSRYFKVEALPLLGSGKLDLKGL 1137
FUM14_C_NRPS-like cd19545
Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond ...
 1088-1399 9.52e-28

Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond forming Fusarium verticillioides FUM14 protein; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) typically catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. However, some C-domains have ester-bond forming activity. This subfamily includes Fusarium verticillioides FUM14 (also known as NRPS8), a bi-domain protein with an ester-bond forming NRPS C-domain, which catalyzes linkages between an aminoacyl/peptidyl-PCP donor and a hydroxyl-containing acceptor. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. FUM14 has an altered active site motif DHTHCD instead of the typical HHxxxD motif seen in other subfamily members. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380467 [Multi-domain]  Cd Length: 395  Bit Score: 118.55  E-value: 9.52e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1088 SQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLGFSLSSHDLrkLDEAarttrvaeLA 1167
Cdd:cd19545    19 PGAYVGQRVFELPPDIDLARLQAAWEQVVQANPILRTRIVQSDSGGLLQVVVKESPISWTESTS--LDEY--------LE 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1168 EQEARaRFDLTQgPLIRGQLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQaaleGSEanlPPLPVQYADYAvwq 1247
Cdd:cd19545    89 EDRAA-PMGLGG-PLVRLALVEDPDTERYFVWTIHHALYDGWSLPLILRQVLAAYQ----GEP---VPQPPPFSRFV--- 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1248 rQWLQGETLNDLRDYWRDQLQGAPALL--EIPTDRPRPSVQRYAgdqvpfhldagQLRRLHALNRQQGTTLFMTLLAAWS 1325
Cdd:cd19545   157 -KYLRQLDDEAAAEFWRSYLAGLDPAVfpPLPSSRYQPRPDATL-----------EHSISLPSSASSGVTLATVLRAAWA 224

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 1326 VVLSRLSGQDDIVIGTPVANR--PRQELEGMVGFFVNTLALRTepgRCHA---VADLLDQVRERALDayahqALPFEQV 1399
Cdd:cd19545   225 LVLSRYTGSDDVVFGVTLSGRnaPVPGIEQIVGPTIATVPLRV---RIDPeqsVEDFLQTVQKDLLD-----MIPFEHT 295
EntF2 COG3319
Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase ...
 1531-2128 1.16e-27

Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442548 [Multi-domain]  Cd Length: 855  Bit Score: 123.27  E-value: 1.16e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDP 1610
Cdd:COG3319     4 AAAAAAAAAAAAAAAAAAAAAAALAAAAAAAAAAALLLLAAALLVALAALALAALALAALLAVALLAAALALAALAALAA 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1611 GYPAERLAYMLDDASPVALLTQANQRALLTGDVPRILLDTADFSHLSEDNPHVPGLDAHH----LAYVIYTSGSTGKPKG 1686
Cdd:COG3319    84 LALALAAAAAALLLAALALLLALLAALALALLALLLAALLLALAALAAAAAAAALAAAAAaaaaLAAAAGLGGGGGGAGV 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1687 VMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDGHKDAAYLAQLIERTGITTLHF 1766
Cdd:COG3319   164 LVLVLAALLALLLAALLALALALAALLLLALAAALALALLLLLALLLLLLLLLALLLLLLLALLAAAALLALLLALLLLL 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1767 VPSMLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHRSfVPIGRPI 1846
Cdd:COG3319   244 LAALLLLLALALLLLLALLLLLGLLALLLALLLLLALLLLAAAAALAAGGTATTAAVTTTAAAAAPGVAGAL-GPIGGGP 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1847 ANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINQPGARLYKTGDLARWLPDGSLEYLGRND 1926
Cdd:COG3319   323 GLLVLLVLLVLLLPLLLGVGGGGGGGGGGGGAGGLAGRGLRAAAALRDPAGAGARGRLRRGGDRGRRLGGGLLLGLGRLR 402

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1927 FQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLcPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLD 2006
Cdd:COG3319   403 LQRLRRGLREELEEAEAALAEAAAVAAAVAAAAAAAAAAAALAAAV-VAAAALAAAALLLLLLLLLLPPPLPPALLLLLL 481

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2007 AFPLTPNGKLDRKALPAPDqsAVATRDYEAPQGEVETALAAVWQDLLGLTRVGRHDHFFALGGHSLLIVSLIERLRRAGL 2086
Cdd:COG3319   482 LLLLLLLAALLLAAAAPAA--AAAAAAAPAPAAALELALALLLLLLLGLGLVGDDDDFFGGGGGSLLALLLLLLLLALLL 559

                         570       580       590       600
                  ....*....|....*....|....*....|....*....|..
gi 641744967 2087 ALDVRGVFSTPVLSDMAQAILAHQDKPAVVVPPNRIPADSTA 2128
Cdd:COG3319   560 RLLLLLALLLAPTLAALAAALAAAAAAAALSPLVPLRAGGSG 601
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
 2608-3094 1.41e-27

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 121.03  E-value: 1.41e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGEASL--SYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVP 2685
Cdd:PRK12583   20 IGDAFDATVARFPDREALVVRHQALryTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVN 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2686 LDPTYPVERLRYMLDDAKPVALIS-----QSAHLGIMNGSLP--------------------VILLDDGETRPFDNEPD- 2739
Cdd:PRK12583  100 INPAYRASELEYALGQSGVRWVICadafkTSDYHAMLQELLPglaegqpgalacerlpelrgVVSLAPAPPPGFLAWHEl 179

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2740 ---------TPLDARKQGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSL--SFDIsI 2808
Cdd:PRK12583  180 qargetvsrEALAERQASLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVPVPLyhCFGM-V 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2809 LEIFLPLLNGARLILATQAQAADAQQLAmlIERHAVSFMQATPSTWRMLVELRDF------ALPPGFKAlcGGEALPEnl 2882
Cdd:PRK12583  259 LANLGCMTVGACLVYPNEAFDPLATLQA--VEEERCTALYGVPTMFIAELDHPQRgnfdlsSLRTGIMA--GAPCPIE-- 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2883 ataLLQKVTTLWNL------YGPTETTIWSTLNGLTTPTPY----IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGV 2952
Cdd:PRK12583  333 ---VMRRVMDEMHMaevqiaYGMTETSPVSLQTTAADDLERrvetVGRTQPHLEVKVVDPDGATVPRGEIGELCTRGYSV 409

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2953 VRGYLGRPDLTAERFITDPFsgapearMYkTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVV 3032
Cdd:PRK12583  410 MKGYWNNPEATAESIDEDGW-------MH-TGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQV 481

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967 3033 IAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDR 3094
Cdd:PRK12583  482 FGVPDEKYGEEIVAWVRLHPGHAASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQK 543
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
 1674-2018 1.57e-27

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 116.60  E-value: 1.57e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1674 VIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFsFDVSVWEFFWPLLY--GARLVMARpdghKDAAY 1751
Cdd:cd17637     5 IIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEADVYLNMLPL-FHIAGLNLALATFHagGANVVMEK----FDPAE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1752 LAQLIERTGITTLHFVPSMLQQFVQWADADCA-CDSLRRVicSGEALPaELQQRFFARFNAQLHNLYGPTEAAIDVTFwa 1830
Cdd:cd17637    80 ALELIEEEKVTLMGSFPPILSNLLDAAEKSGVdLSSLRHV--LGLDAP-ETIQRFEETTGATFWSLYGQTETSGLVTL-- 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1831 cQPDDHRSfVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFipdpfinqpgaR--LYKTG 1908
Cdd:cd17637   155 -SPYRERP-GSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYTF-----------RngWHHTG 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1909 DLARWLPDGSLEYLGRNDFQ--VKLRGFRIELGEIEARLMQCPGVQEAVVVAREDsPGDTRLVAYLCP-QPGVTPDPADL 1985
Cdd:cd17637   222 DLGRFDEDGYLWYAGRKPEKelIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPD-PKWGEGIKAVCVlKPGATLTADEL 300

                         330       340       350
                  ....*....|....*....|....*....|...
gi 641744967 1986 RQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDR 2018
Cdd:cd17637   301 IEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
 1538-2021 2.08e-27

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 119.02  E-value: 2.08e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICverSLDMVIGLLAILKAGAAYVPldpgyPAERL 1617
Cdd:cd05929     2 EARDLDRAQVFHQRRLLLLDVYSIALNRNARAAAAEGVWIADGVYIY---LINSILTVFAAAAAWKCGAC-----PAYKS 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1618 AYMLDDASPVALLTQANQRALLTGDVPRILLDTADFSHLSEDNPHVPGLDAHHLAYVIYTSGSTGKPKGVM--------- 1688
Cdd:cd05929    74 SRAPRAEACAIIEIKAAALVCGLFTGGGALDGLEDYEAAEGGSPETPIEDEAAGWKMLYSGGTTGRPKGIKrglpggppd 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1689 NSHRALCNRLVWMqntyrlTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARpdgHKDAAYLAQLIERTGITTLHFVP 1768
Cdd:cd05929   154 NDTLMAAALGFGP------GADSVYLSPAPLYHAAPFRWSMTALFMGGTLVLME---KFDPEEFLRLIERYRVTFAQFVP 224

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1769 SMLQQFVQWADA-----DCAcdSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEA----AIDVTFWACQPDDhrsf 1839
Cdd:cd05929   225 TMFVRLLKLPEAvrnayDLS--SLKRVIHAAAPCPPWVKEQWIDWGGPIIWEYYGGTEGqgltIINGEEWLTHPGS---- 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1840 vpIGRPIANtQLYILDTLGQPVPLGVAGELHIGGvGVARGYLNRPDLTAERfipdpfINQPGARlyKTGDLARWLPDGSL 1919
Cdd:cd05929   299 --VGRAVLG-KVHILDEDGNEVPPGEIGEVYFAN-GPGFEYTNDPEKTAAA------RNEGGWS--TLGDVGYLDEDGYL 366

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1920 EYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEY 1996
Cdd:cd05929   367 YLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPGADAGTAlaeELIAFLRDRLSRY 446

                         490       500
                  ....*....|....*....|....*
gi 641744967 1997 MVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05929   447 KCPRSIEFVAELPRDDTGKLYRRLL 471
PRK09029 PRK09029
O-succinylbenzoic acid--CoA ligase; Provisional
 483-963 2.31e-27

O-succinylbenzoic acid--CoA ligase; Provisional


Pssm-ID: 236363 [Multi-domain]  Cd Length: 458  Bit Score: 118.82  E-value: 2.31e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:PRK09029   13 AQVRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQPLL 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLTQSAQVAQLNSTLPTVLLDTPAAAACPDtnpvvqglhAAHLAYVIYTSGSTGRPKGVMVAHRNVINL 642
Cdd:PRK09029   93 EELLPSLTLDFALVLEGENTFSALTSLHLQLVEGAHAVAWQ---------PQRLATMTLTSGSTGLPKAAVHTAQAHLAS 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  643 ATGLHTL------------LALDHPSRIAlnasIVfdasvknWIQLLSGHTLVLvpdalrADAHQLWRyfarhavDLFDC 710
Cdd:PRK09029  164 AEGVLSLmpftaqdswllsLPLFHVSGQG----IV-------WRWLYAGATLVV------RDKQPLEQ-------ALAGC 219

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  711 T-----PVQLQWLLDaglgsDPAYQPA--QVLIGGEAISPAVWSRLQSLSDTRFINvYGPTEctVDATACVVdRTQPLPT 783
Cdd:PRK09029  220 ThaslvPTQLWRLLD-----NRSEPLSlkAVLLGGAAIPVELTEQAEQQGIRCWCG-YGLTE--MASTVCAK-RADGLAG 290

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  784 IGKPLANTRLYIldaqdqpvpigVTGELHIGGAGVARGYLHRPDLTaerfipdPFSADPAAriYKTGDLARWLpDGNIDY 863
Cdd:PRK09029  291 VGSPLPGREVKL-----------VDGEIWLRGASLALGYWRQGQLV-------PLVNDEGW--FATRDRGEWQ-NGELTI 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  864 LGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLcaRPDAELHPAALRQQLAASLADYMIPSA 943
Cdd:PRK09029  350 LGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVFVVPVADAEFGQRPVAVV--ESDSEAAVVNLAEWLQDKLARFQQPVA 427

                         490       500
                  ....*....|....*....|
gi 641744967  944 FVTLDALPLTPNGKLDRKAL 963
Cdd:PRK09029  428 YYLLPPELKNGGIKISRQAL 447
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
 490-965 4.21e-27

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 119.61  E-value: 4.21e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  490 IAVLFEDQH----LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYI 565
Cdd:PRK04319   61 VALRYLDASrkekYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDR 140

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  566 LDDAAPVALLTQSAQ-----VAQLnSTLPTVLL------------DTPAAAACPDTNPVVQGLHAAHLAYVIYTSGSTGR 628
Cdd:PRK04319  141 LEDSEAKVLITTPALlerkpADDL-PSLKHVLLvgedveegpgtlDFNALMEQASDEFDIEWTDREDGAILHYTSGSTGK 219

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  629 PKGVMVAHRNVI-NLATGLHtllALD-HPSRI---ALNASIVFDASVKNWIQLLSGHTLVLvpDALRADAHQLWRYFARH 703
Cdd:PRK04319  220 PKGVLHVHNAMLqHYQTGKY---VLDlHEDDVywcTADPGWVTGTSYGIFAPWLNGATNVI--DGGRFSPERWYRILEDY 294

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  704 AVDLFDCTPVQLQWLLDAglGSDPAYQ---PA--QVLIGGEAISP-AVWSRLQSLsDTRFINVYGPTEctvdaTACVVDR 777
Cdd:PRK04319  295 KVTVWYTAPTAIRMLMGA--GDDLVKKydlSSlrHILSVGEPLNPeVVRWGMKVF-GLPIHDNWWMTE-----TGGIMIA 366

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  778 TQPLPTI-----GKPLANTRLYILDAQDQPVPIGVTGELHI--GGAGVARGYLHRPDLTAERFIPDpfsadpaarIYKTG 850
Cdd:PRK04319  367 NYPAMDIkpgsmGKPLPGIEAAIVDDQGNELPPNRMGNLAIkkGWPSMMRGIWNNPEKYESYFAGD---------WYVSG 437

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  851 DLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAElhPA-ALRQ 929
Cdd:PRK04319  438 DSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYE--PSeELKE 515

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967  930 QLAA----SLADYMIPSAFVTLDALPLTPNGKLDRKALPA 965
Cdd:PRK04319  516 EIRGfvkkGLGAHAAPREIEFKDKLPKTRSGKIMRRVLKA 555
PRK07638 PRK07638
acyl-CoA synthetase; Validated
 473-963 5.11e-27

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 118.34  E-value: 5.11e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  473 MLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDdRVALCVERSLEMMVGLLGILKAGAAYVP 552
Cdd:PRK07638    1 MGITKEYKKHASLQPNKIAIKENDRVLTYKDWFESVCKVANWLNEKESKNK-TIAILLENRIEFLQLFAGAAMAGWTCVP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  553 MDPAYPAERLAYILDDAAPVALLTQSAQVAQL-NSTLPTVLLDT---PAAAACPDTNPVVQGLHAAHlaYVIYTSGSTGR 628
Cdd:PRK07638   80 LDIKWKQDELKERLAISNADMIVTERYKLNDLpDEEGRVIEIDEwkrMIEKYLPTYAPIENVQNAPF--YMGFTSGSTGK 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  629 PKGVMVAHRnvinlaTGLHTllaldhpsrialnasivFDASVKNWiqLLSGHTLVLVPDALRadaHQLWRYFARHAvdLF 708
Cdd:PRK07638  158 PKAFLRAQQ------SWLHS-----------------FDCNVHDF--HMKREDSVLIAGTLV---HSLFLYGAIST--LY 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  709 DCTPVQLQwlldaglgsdPAYQPAQVL--IGGEAI----------------------------SPAVWS-----RLQSL- 752
Cdd:PRK07638  208 VGQTVHLM----------RKFIPNQVLdkLETENIsvmytvptmleslykenrvienkmkiisSGAKWEaeakeKIKNIf 277

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  753 SDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHrpdltaER 832
Cdd:PRK07638  278 PYAKLYEFYGASELSFVTALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTVYVKSPQFFMGYII------GG 351

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  833 FIPDPFSADPAARIYKTGDLARwlpDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVA 912
Cdd:PRK07638  352 VLARELNADGWMTVRDVGYEDE---EGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVA 428

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 641744967  913 YLCARPDAELHPAALRQQLAAsladYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK07638  429 IIKGSATKQQLKSFCLQRLSS----FKIPKEWHFVDEIPYTNSGKIARMEA 475
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
 2631-3097 7.07e-27

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 117.82  E-value: 7.07e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHhLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDA--KPV--- 2705
Cdd:cd05909     7 SLTYRKLLTGAIALAR-KLAKMTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAgiKTVlts 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2706 -ALISQSAHLGIMNGSLP--VILLDD----------------GETRPfdnepdtPLDARKQGLTPRH---LAYVIYTSGS 2763
Cdd:cd05909    86 kQFIEKLKLHHLFDVEYDarIVYLEDlrakiskadkckaflaGKFPP-------KWLLRIFGVAPVQpddPAVILFTSGS 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2764 TGKPKGVMVEHANMVNFL--CSMRKEPgiAQEDVLLGVTSL--SFDISIlEIFLPLLNGARLILATQAQAADAQQLamLI 2839
Cdd:cd05909   159 EGLPKGVVLSHKNLLANVeqITAIFDP--NPEDVVFGALPFfhSFGLTG-CLWLPLLSGIKVVFHPNPLDYKKIPE--LI 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2840 ERHAVSFMQATPSTWRMLVElrdFALPPGFKAL----CGGEALPENLATALLQKV-TTLWNLYGPTETtiwSTLNGLTTP 2914
Cdd:cd05909   234 YDKKATILLGTPTFLRGYAR---AAHPEDFSSLrlvvAGAEKLKDTLRQEFQEKFgIRILEGYGTTEC---SPVISVNTP 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2915 TP-----YIGHPIANTQIYILDAQGRV-VPLGVAGEIHIAGAGVVRGYLGRPDLTAErfitdpfsgAPEARMYKTGDLGR 2988
Cdd:cd05909   308 QSpnkegTVGRPLPGMEVKIVSVETHEeVPIGEGGLLLVRGPNVMLGYLNEPELTSF---------AFGDGWYDTGDIGK 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2989 WLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLA---RCHGVHDAVVIAREdSPGDKRLVAYLlaqpDTVLEPADLRQRL 3065
Cdd:cd05909   379 IDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSeilPEDNEVAVVSVPDG-RKGEKIVLLTT----TTDTDPSSLNDIL 453

                         490       500       510
                  ....*....|....*....|....*....|...
gi 641744967 3066 SE-GVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05909   454 KNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTL 486
C_NRPS-like cd19066
Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of ...
 29-440 7.14e-27

Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long, with various activities such as antibiotic, antifungal, antitumor and immunosuppression. There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380453 [Multi-domain]  Cd Length: 427  Bit Score: 116.74  E-value: 7.14e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   29 YPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICwQGLHQPVQVVWRQapltvnt 108
Cdd:cd19066     2 IPLSPMQRGMWFLKKLATDPSAFNVAIEMFLTGSLDLARLKQALDAVMERHDVLRTRFC-EEAGRYEQVVLDK------- 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  109 ltTTSSDTVPAQLRAATDPSNHRLNLSNAPLLSAT-TAHDPVC---------GEWLLSLSIHHLISDHIT-QALIIDEIR 177
Cdd:cd19066    74 --TVRFRIEIIDLRNLADPEARLLELIDQIQQTIYdLERGPLVrvalfrladERDVLVVAIHHIIVDGGSfQILFEDISS 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  178 L---LLEDRPEALPKPLPYRNFIAQILSVPLSEHEQ----YFRNRLADIdTPTAPF--DLVDVQGNGEDITEARLSLDSS 248
Cdd:cd19066   152 VydaAERQKPTLPPPVGSYADYAAWLEKQLESEAAQadlaYWTSYLHGL-PPPLPLpkAKRPSQVASYEVLTLEFFLRSE 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  249 LADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLqgNLGADRVMGMFINTLPLRVSL-RERSVHDVV 327
Cdd:cd19066   231 ETKRLREVARESGTTPTQLLLAAFALALKRLTASIDVVIGLTFLNRP--DEAVEDTIGLFLNLLPLRIDTsPDATFPELL 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  328 QATSHELMMLLAHEQAPLALAQQCSQVPPPL---PLFSTLFNYR--HSQKDASSQFWEGMRQLSGRERTNYPITLSV-DD 401
Cdd:cd19066   309 KRTKEQSREAIEHQRVPFIELVRHLGVVPEApkhPLFEPVFTFKnnQQQLGKTGGFIFTTPVYTSSEGTVFDLDLEAsED 388

                         410       420       430
                  ....*....|....*....|....*....|....*....
gi 641744967  402 LGDGFNLTAKTVMGVDPERIVHYMLTAIENLVTSLEKTP 440
Cdd:cd19066   389 PDGDLLLRLEYSRGVYDERTIDRFAERYMTALRQLIENP 427
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
 2608-3102 8.13e-27

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 118.60  E-value: 8.13e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2608 IHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLD 2687
Cdd:PRK06710   26 LHKYVEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTN 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2688 PTYPVERLRYMLDDAKPVALI------SQSAHLGIMNGSLPVILLDDGETRPFDNEPDTPLDARKQG------------- 2748
Cdd:PRK06710  106 PLYTERELEYQLHDSGAKVILcldlvfPRVTNVQSATKIEHVIVTRIADFLPFPKNLLYPFVQKKQSnlvvkvsesetih 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2749 ----------------LTPRH-LAYVIYTSGSTGKPKGVMVEHANMV-NFLCSMRKEPGIAQ-EDVLLGVTSLSFDISIL 2809
Cdd:PRK06710  186 lwnsvekevntgvevpCDPENdLALLQYTGGTTGFPKGVMLTHKNLVsNTLMGVQWLYNCKEgEEVVLGVLPFFHVYGMT 265

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2810 EIF-LPLLNGARLILATQAQAADAQQLamlIERHAVSFMQATPSTWRMLVE---LRDFALPPGFKALCGGEALPENLATA 2885
Cdd:PRK06710  266 AVMnLSIMQGYKMVLIPKFDMKMVFEA---IKKHKVTLFPGAPTIYIALLNsplLKEYDISSIRACISGSAPLPVEVQEK 342

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2886 LlQKVT--TLWNLYGPTETTIWSTLNGL---TTPTPyIGHPIANTQIYILDAQ-GRVVPLGVAGEIHIAGAGVVRGYLGR 2959
Cdd:PRK06710  343 F-ETVTggKLVEGYGLTESSPVTHSNFLwekRVPGS-IGVPWPDTEAMIMSLEtGEALPPGEIGEIVVKGPQIMKGYWNK 420

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2960 PDLTAErFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSP 3039
Cdd:PRK06710  421 PEETAA-VLQDGW--------LHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPY 491

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967 3040 GDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPDQ 3102
Cdd:PRK06710  492 RGETVKAFVVLKEGTECSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIEEEK 554
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 2630-3097 8.65e-27

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 116.79  E-value: 8.65e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2630 ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALIs 2709
Cdd:cd05910     1 SRLSFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKNLKQCLQEAEPDAFI- 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2710 qsahlGImngslpvillddgetrPFDNEPdtpldarkqgltprhlAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPG 2789
Cdd:cd05910    80 -----GI----------------PKADEP----------------AAILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYG 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2790 IAQEDVLLGVTSLsfdisiLEIFLPLLNGARLILATQAQAADAQQLAML---IERHAVSFMQATPSTWRMLV---ELRDF 2863
Cdd:cd05910   123 IRPGEVDLATFPL------FALFGPALGLTSVIPDMDPTRPARADPQKLvgaIRQYGVSIVFGSPALLERVArycAQHGI 196

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2864 ALPPGFKALCGGEALPENLATAL---LQKVTTLWNLYGPTETTIWSTLNG---LTTPTPY--------IGHPIANTQIYI 2929
Cdd:cd05910   197 TLPSLRRVLSAGAPVPIALAARLrkmLSDEAEILTPYGATEALPVSSIGSrelLATTTAAtsggagtcVGRPIPGVRVRI 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2930 LDA---------QGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGApearMYKTGDLGRWLPDGTLEYLGR 3000
Cdd:cd05910   277 IEIddepiaewdDTLELPRGEIGEITVTGPTVTPTYVNRPVATALAKIDDNSEGF----WHRMGDLGYLDDEGRLWFCGR 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3001 NDFQVKVRGFRIELGEIEtrlaRCHGVHDAV---VIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEY---MI 3074
Cdd:cd05910   353 KAHRVITTGGTLYTEPVE----RVFNTHPGVrrsALVGVGKPGCQLPVLCVEPLPGTITPRARLEQELRALAKDYphtQR 428

                         490       500
                  ....*....|....*....|....*
gi 641744967 3075 PSAFVTLDAFPLTP--NGKLDRKAL 3097
Cdd:cd05910   429 IGRFLIHPSFPVDIrhNAKIFREKL 453
PRK08315 PRK08315
AMP-binding domain protein; Validated
 471-957 9.93e-27

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 118.37  E-value: 9.93e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  471 REMLIQQRFEAQAEQTPEAIAVLFEDQHL--TYRELNRRANQLAHHLIALGVQPDDRVAL----CVERSLEMmvglLGIL 544
Cdd:PRK08315   14 LEQTIGQLLDRTAARYPDREALVYRDQGLrwTYREFNEEVDALAKGLLALGIEKGDRVGIwapnVPEWVLTQ----FATA 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  545 KAGAAYVPMDPAYPAERLAYILDDAAPVALLT--------------------QSAQVAQLNS-TLP----TVLLDTPAAA 599
Cdd:PRK08315   90 KIGAILVTINPAYRLSELEYALNQSGCKALIAadgfkdsdyvamlyelapelATCEPGQLQSaRLPelrrVIFLGDEKHP 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  600 ACPDTNPVVQ-GLHAAHLAY-----------VI---YTSGSTGRPKGVMVAHRNVINlaTGLHT--LLALDHPSRIALNa 662
Cdd:PRK08315  170 GMLNFDELLAlGRAVDDAELaarqatldpddPIniqYTSGTTGFPKGATLTHRNILN--NGYFIgeAMKLTEEDRLCIP- 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  663 siV-----FDASVKNWIQLLSGHTLVLVPDA------LRADAHQlwRYFARHAV-------------DLFDctpvqlqwl 718
Cdd:PRK08315  247 --VplyhcFGMVLGNLACVTHGATMVYPGEGfdplatLAAVEEE--RCTALYGVptmfiaeldhpdfARFD--------- 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  719 ldagLGSdpayqpaqvLIGG-EAISP---AVWSRLQSLSDTRFIN-VYGPTECTVDATACVVD-----RTQplpTIGKPL 788
Cdd:PRK08315  314 ----LSS---------LRTGiMAGSPcpiEVMKRVIDKMHMSEVTiAYGMTETSPVSTQTRTDdplekRVT---TVGRAL 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  789 ANTRLYILDAQD-QPVPIGVTGELHIGGAGVARGYLHRPDLTAErfipdpfsADPAARIYKTGDLARWLPDGNIDYLGRn 867
Cdd:PRK08315  378 PHLEVKIVDPETgETVPRGEQGELCTRGYSVMKGYWNDPEKTAE--------AIDADGWMHTGDLAVMDEEGYVNIVGR- 448

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  868 dfqIK---VRGfrieaGE------IESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADY 938
Cdd:PRK08315  449 ---IKdmiIRG-----GEniypreIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEEDVRDFCRGKIAHY 520

                         570
                  ....*....|....*....
gi 641744967  939 MIPSAFVTLDALPLTPNGK 957
Cdd:PRK08315  521 KIPRYIRFVDEFPMTVTGK 539
PRK07008 PRK07008
long-chain-fatty-acid--CoA ligase; Validated
 500-958 1.15e-26

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235908 [Multi-domain]  Cd Length: 539  Bit Score: 117.89  E-value: 1.15e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  500 TYRELNRRANQLAHHLIALGVQPDDRVALCV---ERSLEMMVGLLGilkAGAAYVPMDPAYPAERLAYILDDAAPVAL-- 574
Cdd:PRK07008   41 TYRDCERRAKQLAQALAALGVEPGDRVGTLAwngYRHLEAYYGVSG---SGAVCHTINPRLFPEQIAYIVNHAEDRYVlf 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  575 -LTQSAQVAQLNSTLPTV---LLDTPAAAACPDTNPVV--QGLHAAH-------------LAYVIYTSGSTGRPKGVMVA 635
Cdd:PRK07008  118 dLTFLPLVDALAPQCPNVkgwVAMTDAAHLPAGSTPLLcyETLVGAQdgdydwprfdenqASSLCYTSGTTGNPKGALYS 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  636 HRnvinlATGLHTLLALdHPSRIALNAS-----IVFDASVKNW----IQLLSGHTLVLVPDALraDAHQLWRYFARHAVD 706
Cdd:PRK07008  198 HR-----STVLHAYGAA-LPDAMGLSARdavlpVVPMFHVNAWglpySAPLTGAKLVLPGPDL--DGKSLYELIEAERVT 269

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  707 LFDCTPVQLQWLLD----AGLGSDPAyqpAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACVV------- 775
Cdd:PRK07008  270 FSAGVPTVWLGLLNhmreAGLRFSTL---RRTVIGGSACPPAMIRTFEDEYGVEVIHAWGMTEMSPLGTLCKLkwkhsql 346

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  776 ---DRTQPLPTIGKPLANTRLYILDAQDQPVPI-GVT-GELHIGGAGVARGYLHRpdltaerfipdpfSADPAAR-IYKT 849
Cdd:PRK07008  347 pldEQRKLLEKQGRVIYGVDMKIVGDDGRELPWdGKAfGDLQVRGPWVIDRYFRG-------------DASPLVDgWFPT 413

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  850 GDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQ 929
Cdd:PRK07008  414 GDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGAEVTREELLA 493

                         490       500
                  ....*....|....*....|....*....
gi 641744967  930 QLAASLADYMIPSAFVTLDALPLTPNGKL 958
Cdd:PRK07008  494 FYEGKVAKWWIPDDVVFVDAIPHTATGKL 522
PRK07008 PRK07008
long-chain-fatty-acid--CoA ligase; Validated
 1555-2016 1.54e-26

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235908 [Multi-domain]  Cd Length: 539  Bit Score: 117.50  E-value: 1.54e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1555 TYDALNRRANQLAHHLIDLGVKPDDRIAICV---ERSLDMVIGllaILKAGAAYVPLDPGYPAERLAYMLDDASPVALLT 1631
Cdd:PRK07008   41 TYRDCERRAKQLAQALAALGVEPGDRVGTLAwngYRHLEAYYG---VSGSGAVCHTINPRLFPEQIAYIVNHAEDRYVLF 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1632 QANQRALLTGDVPR-------ILLdtADFSHLSEDN-PHV---------------PGLDAHHLAYVIYTSGSTGKPKGVM 1688
Cdd:PRK07008  118 DLTFLPLVDALAPQcpnvkgwVAM--TDAAHLPAGStPLLcyetlvgaqdgdydwPRFDENQASSLCYTSGTTGNPKGAL 195

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1689 NSHRA--LCNRLVWMQNTYRLTPDDRVLQKTPFsFDVSVWEFFWPL-LYGARLVMARPDghKDAAYLAQLIERTGITTLH 1765
Cdd:PRK07008  196 YSHRStvLHAYGAALPDAMGLSARDAVLPVVPM-FHVNAWGLPYSApLTGAKLVLPGPD--LDGKSLYELIEAERVTFSA 272

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1766 FVPS---MLQQFVQWADADCAcdSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTF----WACQ--PDD- 1835
Cdd:PRK07008  273 GVPTvwlGLLNHMREAGLRFS--TLRRTVIGGSACPPAMIRTFEDEYGVEVIHAWGMTEMSPLGTLcklkWKHSqlPLDe 350

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1836 -HRSFVPIGRPIANTQLYILDTLGQPVPL-GVA-GELHIGGVGVARGYLNRPDltaerfipDPFINQpgarLYKTGDLAR 1912
Cdd:PRK07008  351 qRKLLEKQGRVIYGVDMKIVGDDGRELPWdGKAfGDLQVRGPWVIDRYFRGDA--------SPLVDG----WFPTGDVAT 418

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1913 WLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGvtpdpADL-RQQLGQ 1991
Cdd:PRK07008  419 IDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPG-----AEVtREELLA 493

                         490       500
                  ....*....|....*....|....*....
gi 641744967 1992 H----LAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:PRK07008  494 FyegkVAKWWIPDDVVFVDAIPHTATGKL 522
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
 1674-2017 1.86e-26

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 113.17  E-value: 1.86e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1674 VIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFsFDVSVWEFFWPLLY--GARLVMARpdghKDAAY 1751
Cdd:cd17636     5 AIYTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGTVFLNSGPL-FHIGTLMFTLATFHagGTNVFVRR----VDAEE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1752 LAQLIERTGITTLHFVPSMLQQFVQwADADCACD--SLRRVICSGE--ALPAELQQRFFARFNAqlhnlYGPTEAAIDVT 1827
Cdd:cd17636    80 VLELIEAERCTHAFLLPPTIDQIVE-LNADGLYDlsSLRSSPAAPEwnDMATVDTSPWGRKPGG-----YGQTEVMGLAT 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1828 FWACQPDDHRSFvpiGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFipdpfinqpGARLYKT 1907
Cdd:cd17636   154 FAALGGGAIGGA---GRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRT---------RGGWHHT 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1908 GDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQ 1987
Cdd:cd17636   222 NDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGASVTEAELIE 301

                         330       340       350
                  ....*....|....*....|....*....|
gi 641744967 1988 QLGQHLAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:cd17636   302 HCRARIASYKKPKSVEFADALPRTAGGADD 331
PRK07788 PRK07788
acyl-CoA synthetase; Validated
 2620-3101 2.03e-26

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 117.34  E-value: 2.03e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:PRK07788   63 PDRAALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQLAEVA 142

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQSAHLGIM------NGSLPVILLDDGETRPFDNEPDTpLD-------ARKQGLTPRHLAYVIYTSGSTGK 2766
Cdd:PRK07788  143 AREGVKALVYDDEFTDLLsalppdLGRLRAWGGNPDDDEPSGSTDET-LDdliagssTAPLPKPPKPGGIVILTSGTTGT 221

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2767 PKGVMvehanmvnflcsmRKEPGIAQEDVLL---------GVTSLSFDI------SILEIFLPLlnGARLILATQAQAAD 2831
Cdd:PRK07788  222 PKGAP-------------RPEPSPLAPLAGLlsrvpfragETTLLPAPMfhatgwAHLTLAMAL--GSTVVLRRRFDPEA 286

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2832 AQQlamLIERHAVSFMQATPSTWRMLVELRDFALP-PGFKAL----CGGEALPENLATALLQKV-TTLWNLYGPTE---T 2902
Cdd:PRK07788  287 TLE---DIAKHKATALVVVPVMLSRILDLGPEVLAkYDTSSLkiifVSGSALSPELATRALEAFgPVLYNLYGSTEvafA 363

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2903 TIWSTLNGLTTPTPyIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYlgrpdltaerfiTDPFSGAPEARMYK 2982
Cdd:PRK07788  364 TIATPEDLAEAPGT-VGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGY------------TDGRDKQIIDGLLS 430

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2983 TGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLR 3062
Cdd:PRK07788  431 SGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGAALDEDAIK 510

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967 3063 QRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPD 3101
Cdd:PRK07788  511 DYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREMD 549
PRK09274 PRK09274
peptide synthase; Provisional
 475-866 2.17e-26

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 116.92  E-value: 2.17e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  475 IQQRFEAQAEQTPEAIAVLFED----------QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGIL 544
Cdd:PRK09274    8 IARHLPRAAQERPDQLAVAVPGgrgadgklayDELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALF 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  545 KAGAAYVPMDPAYPAERLAYILDDAAPVALLTQ-SAQVAQL----------------------NSTLPTVLLDTPAAA-A 600
Cdd:PRK09274   88 KAGAVPVLVDPGMGIKNLKQCLAEAQPDAFIGIpKAHLARRlfgwgkpsvrrlvtvggrllwgGTTLATLLRDGAAAPfP 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  601 CPDTNPvvqglhaAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSR------------IAL-NASIVFD 667
Cdd:PRK09274  168 MADLAP-------DDMAAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIdlptfplfalfgPALgMTSVIPD 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  668 AsvknwiqllsghtlvlvpDALR---ADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSD---PAYQpaQVLIGGEAI 741
Cdd:PRK09274  241 M------------------DPTRpatVDPAKLFAAIERYGVTNLFGSPALLERLGRYGEANGiklPSLR--RVISAGAPV 300

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  742 SPAVWSRLQSL--SDTRFINVYGPTEC--------------TVDATA-----CVvdrtqplptiGKPLANTRLYILD--- 797
Cdd:PRK09274  301 PIAVIERFRAMlpPDAEILTPYGATEAlpissiesreilfaTRAATDngagiCV----------GRPVDGVEVRIIAisd 370

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967  798 ------AQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPdpfsaDPAARIY-KTGDLARWLPDGNIDYLGR 866
Cdd:PRK09274  371 apipewDDALRLATGEIGEIVVAGPMVTRSYYNRPEATRLAKIP-----DGQGDVWhRMGDLGYLDAQGRLWFCGR 441
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
 2600-3097 4.33e-26

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 116.27  E-value: 4.33e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2600 ADIP--RHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGIL 2677
Cdd:PRK07059   15 AEIDasQYPSLADLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVL 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2678 KAGGAYVPLDPTYPVERLRYMLDD--AKPVALISQSAH------------------LGIMNG------------------ 2719
Cdd:PRK07059   95 RAGYVVVNVNPLYTPRELEHQLKDsgAEAIVVLENFATtvqqvlaktavkhvvvasMGDLLGfkghivnfvvrrvkkmvp 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2720 --SLPVIL-----LDDGETRPFdnepdtpldaRKQGLTPRHLAYVIYTSGSTGKPKGVMVEHANMV-NFLCS-------M 2784
Cdd:PRK07059  175 awSLPGHVrfndaLAEGARQTF----------KPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVaNVLQMeawlqpaF 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2785 RKEPGIaqeDVLLGVTSLS-FDISILEI--FLPLLNGARLILATQAQAADAQQLAMliERHAVSFMQATPSTWRMLVELR 2861
Cdd:PRK07059  245 EKKPRP---DQLNFVCALPlYHIFALTVcgLLGMRTGGRNILIPNPRDIPGFIKEL--KKYQVHIFPAVNTLYNALLNNP 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2862 DFA---LPPGFKALCGGEALPENLATALLQKV-TTLWNLYGPTETTIWSTLNGLTTP--TPYIGHPIANTQIYILDAQGR 2935
Cdd:PRK07059  320 DFDkldFSKLIVANGGGMAVQRPVAERWLEMTgCPITEGYGLSETSPVATCNPVDATefSGTIGLPLPSTEVSIRDDDGN 399

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2936 VVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELG 3015
Cdd:PRK07059  400 DLPLGEPGEICIRGPQVMAGYWNRPDETAKVMTADGF--------FRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPN 471

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3016 EIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQpDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRK 3095
Cdd:PRK07059  472 EIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFVVKK-DPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRR 550


                  ..
gi 641744967 3096 AL 3097
Cdd:PRK07059  551 EL 552
PRK13382 PRK13382
bile acid CoA ligase;
472-966 6.30e-26

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 115.63  E-value: 6.30e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  472 EMLIQQRFEAQAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYV 551
Cdd:PRK13382   42 GMGPTSGFAIAAQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADIL 121

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  552 PMDPAYPAERLAYILDDAAPVALLTQSaqvaQLNSTLPTVLLDTPAAA---ACPDT--NPVVQGLHAAHLA--------- 617
Cdd:PRK13382  122 LLNTSFAGPALAEVVTREGVDTVIYDE----EFSATVDRALADCPQATrivAWTDEdhDLTVEVLIAAHAGqrpeptgrk 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  618 --YVIYTSGSTGRPKGvmvAHRNVINLATGLHTLLaldhpSRIALNAS--IVFDASV-KNWiqllsGHTLVLVPDALRAD 692
Cdd:PRK13382  198 grVILLTSGTTGTPKG---ARRSGPGGIGTLKAIL-----DRTPWRAEepTVIVAPMfHAW-----GFSQLVLAASLACT 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  693 AHQLWRYFARHAVDLFD--------CTPVQLQWLLDAglgsdpayqPAQVL------------IGGEAISPAVWSRLQSL 752
Cdd:PRK13382  265 IVTRRRFDPEATLDLIDrhratglaVVPVMFDRIMDL---------PAEVRnrysgrslrfaaASGSRMRPDVVIAFMDQ 335

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  753 SDTRFINVYGPTECTVDATACVVD-RTQPlPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYlhRPDLTAE 831
Cdd:PRK13382  336 FGDVIYNNYNATEAGMIATATPADlRAAP-DTAGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGY--TSGSTKD 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  832 rfIPDPFSAdpaariykTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLV 911
Cdd:PRK13382  413 --FHDGFMA--------SGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLA 482

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967  912 AYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAP 966
Cdd:PRK13382  483 AFVVLKPGASATPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQAR 537
PRK07529 PRK07529
AMP-binding domain protein; Validated
 483-963 6.54e-26

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 116.21  E-value: 6.54e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLF--------EDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAyVPMD 554
Cdd:PRK07529   35 AARHPDAPALSFlldadpldRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGGEAAGIA-NPIN 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  555 PAYPAERLAYILDDAAPVALLT----------QSAQ--VAQLNSTLPTVLLDTPAAAACPDTNPVVQGLHAAHLAYVIY- 621
Cdd:PRK07529  114 PLLEPEQIAELLRAAGAKVLVTlgpfpgtdiwQKVAevLAALPELRTVVEVDLARYLPGPKRLAVPLIRRKAHARILDFd 193

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  622 ---------------------------TSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRI--ALNASIVFDASVKN 672
Cdd:PRK07529  194 aelarqpgdrlfsgrpigpddvaayfhTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPGDTVfcGLPLFHVNALLVTG 273

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  673 WIQLLSGHTLVL-VPDALRADA--HQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRL 749
Cdd:PRK07529  274 LAPLARGAHVVLaTPQGYRGPGviANFWKIVERYRINFLSGVPTVYAALLQVPVDGHDISSLRYALCGAAPLPVEVFRRF 353

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  750 QSLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYI--LDAQD---QPVPIGVTGELHIGGAGVARGYL- 823
Cdd:PRK07529  354 EAATGVRIVEGYGLTEATCVSSVNPPDGERRIGSVGLRLPYQRVRVviLDDAGrylRDCAVDEVGVLCIAGPNVFSGYLe 433

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  824 --HRPDLTAERfipdpfsadpaaRIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAR 901
Cdd:PRK07529  434 aaHNKGLWLED------------GWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLRHPAVALAAAVGR 501

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967  902 EDSPGDTRLVAYLCARPDAELHPAALRQQLAASLAD-YMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK07529  502 PDAHAGELPVAYVQLKPGASATEAELLAFARDHIAErAAVPKHVRILDALPKTAVGKIFKPAL 564
PLN02246 PLN02246
4-coumarate--CoA ligase
 1554-2023 6.57e-26

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 115.46  E-value: 6.57e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA 1633
Cdd:PLN02246   51 YTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKASGAKLIITQS 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 NQRALLTG-----DVPRILLDTA-----DFSHLSE-DNPHVPGLDAHHLAYVI--YTSGSTGKPKGVMNSHRALCNRlVW 1700
Cdd:PLN02246  131 CYVDKLKGlaeddGVTVVTIDDPpegclHFSELTQaDENELPEVEISPDDVVAlpYSSGTTGLPKGVMLTHKGLVTS-VA 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1701 MQ------NTYrLTPDDRVLQKTP----FSFDvSVweffwpLLYGAR-----LVMARpdghKDAAYLAQLIERTGITTLH 1765
Cdd:PLN02246  210 QQvdgenpNLY-FHSDDVILCVLPmfhiYSLN-SV------LLCGLRvgaaiLIMPK----FEIGALLELIQRHKVTIAP 277

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1766 FVPSMLQQFVQWADADCACDSLRRVICSGEA-LPAELQQRFFARF-NAQLHNLYGPTEA----AIDVTFwACQPDDHRSF 1839
Cdd:PLN02246  278 FVPPIVLAIAKSPVVEKYDLSSIRMVLSGAApLGKELEDAFRAKLpNAVLGQGYGMTEAgpvlAMCLAF-AKEPFPVKSG 356

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1840 vPIGRPIANTQLYILDT-LGQPVPLGVAGELHIGGVGVARGYLNRPDLTAErfipdpfinqpgarlykTGDLARWLPDGS 1918
Cdd:PLN02246  357 -SCGTVVRNAELKIVDPeTGASLPRNQPGEICIRGPQIMKGYLNDPEATAN-----------------TIDKDGWLHTGD 418

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1919 LEYLGRND--FQV-------KLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQL 1989
Cdd:PLN02246  419 IGYIDDDDelFIVdrlkeliKYKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITEDEIKQFV 498

                         490       500       510
                  ....*....|....*....|....*....|....
gi 641744967 1990 GQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PLN02246  499 AKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLRA 532
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 2759-3094 6.65e-26

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 111.99  E-value: 6.65e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2759 YTSGSTGKPKGVMVEHANMVN--FLCSMRKepGIAQEDVLLGVTSLsFDI--SILEIFLPLLNGARLILATQAQAADAQQ 2834
Cdd:cd05917     9 FTSGTTGSPKGATLTHHNIVNngYFIGERL--GLTEQDRLCIPVPL-FHCfgSVLGVLACLTHGATMVFPSPSFDPLAVL 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2835 LAmlIERHAVSFMQATPSTWRMLVELRDFALPPgFKALCGGEALPENLATALLQKVTTLWNL------YGPTETTIWSTL 2908
Cdd:cd05917    86 EA--IEKEKCTALHGVPTMFIAELEHPDFDKFD-LSSLRTGIMAGAPCPPELMKRVIEVMNMkdvtiaYGMTETSPVSTQ 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 NGLTTPTPY----IGHPIANTQIYILDAQGRVVP-LGVAGEIHIAGAGVVRGYLGRPDLTAERFITDpfsgapeaRMYKT 2983
Cdd:cd05917   163 TRTDDSIEKrvntVGRIMPHTEAKIVDPEGGIVPpVGVPGELCIRGYSVMKGYWNDPEKTAEAIDGD--------GWLHT 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2984 GDLGRWLPDGTLEYLGRndfqVK---VRGFR-IELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPA 3059
Cdd:cd05917   235 GDLAVMDEDGYCRIVGR----IKdmiIRGGEnIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEGAELTEE 310

                         330       340       350
                  ....*....|....*....|....*....|....*
gi 641744967 3060 DLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDR 3094
Cdd:cd05917   311 DIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQK 345
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 1554-2021 7.45e-26

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 113.82  E-value: 7.45e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQA 1633
Cdd:cd05974     1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRVDRGGAVYAAVDE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1634 NQRAlltgDVPRILLdtadfshlsednphvpgldahhlayviYTSGSTGKPKGVMNSHRAL----CNRLVWMQntyrLTP 1709
Cdd:cd05974    81 NTHA----DDPMLLY---------------------------FTSGTTSKPKLVEHTHRSYpvghLSTMYWIG----LKP 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1710 DDRVLQKTPFSFDVSVWE-FFWPLLYGArLVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQwADADCACDSLR 1788
Cdd:cd05974   126 GDVHWNISSPGWAKHAWScFFAPWNAGA-TVFLFNYARFDAKRVLAALVRYGVTTLCAPPTVWRMLIQ-QDLASFDVKLR 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1789 RVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHRSfvpIGRPIANTQLYILDTLGQPVPLGVAGe 1868
Cdd:cd05974   204 EVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQTETTALVGNSPGQPVKAGS---MGRPLPGYRVALLDPDGAPATEGEVA- 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1869 LHIGG---VGVARGYLNRPDLTAERFipdpfinqpGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARL 1945
Cdd:cd05974   280 LDLGDtrpVGLMKGYAGDPDKTAHAM---------RGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVL 350

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1946 MQCPGVQEAVVVAredSPGDTRLV---AYLCPQPGVTPDPaDLRQQLGQHLAEYMVPGAFVTLDAF---PLTPNGKLDRK 2019
Cdd:cd05974   351 IEHPAVAEAAVVP---SPDPVRLSvpkAFIVLRAGYEPSP-ETALEIFRFSRERLAPYKRIRRLEFaelPKTISGKIRRV 426


                  ..
gi 641744967 2020 AL 2021
Cdd:cd05974   427 EL 428
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
 482-963 7.97e-26

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 115.74  E-value: 7.97e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  482 QAEQTPEAIAVLFE------DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDP 555
Cdd:cd05966    62 HLKERGDKVAIIWEgdepdqSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFA 141

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  556 AYPAERLAYILDDAAPVALLTQSA---------------QVAQLNSTLPTVL----LDTPAA-------------AACPD 603
Cdd:cd05966   142 GFSAESLADRINDAQCKLVITADGgyrggkviplkeivdEALEKCPSVEKVLvvkrTGGEVPmtegrdlwwhdlmAKQSP 221

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  604 TNPVVQgLHAAHLAYVIYTSGSTGRPKGVMvaHrnvinlATGLHTLLAldhpsriALNASIVFD----------ASVkNW 673
Cdd:cd05966   222 ECEPEW-MDSEDPLFILYTSGSTGKPKGVV--H------TTGGYLLYA-------ATTFKYVFDyhpddiywctADI-GW 284

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  674 IqllSGHT-LVLVPDALRA------------DAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQV-LIG-- 737
Cdd:cd05966   285 I---TGHSyIVYGPLANGAttvmfegtptypDPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLrVLGsv 361

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  738 GEAISPAVWsrlqslsdTRFINVYGPTECTVdatacvVD---RTQ-------PLP--TIGKPLANTRLY------ILDAQ 799
Cdd:cd05966   362 GEPINPEAW--------MWYYEVIGKERCPI------VDtwwQTEtggimitPLPgaTPLKPGSATRPFfgiepaILDEE 427

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  800 DQPVPIGVTGELHIGGA--GVARGyLHRPDltaERFIPDPFSADPAarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFR 877
Cdd:cd05966   428 GNEVEGEVEGYLVIKRPwpGMART-IYGDH---ERYEDTYFSKFPG--YYFTGDGARRDEDGYYWITGRVDDVINVSGHR 501

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  878 IEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPA---ALRQQLAASLADYMIPSAFVTLDALPLTP 954
Cdd:cd05966   502 LGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSDElrkELRKHVRKEIGPIATPDKIQFVPGLPKTR 581


                  ....*....
gi 641744967  955 NGKLDRKAL 963
Cdd:cd05966   582 SGKIMRRIL 590
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
 1534-2016 1.21e-25

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 114.87  E-value: 1.21e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1534 VEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYP 1613
Cdd:PRK07786   23 LARHALMQPDAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLT 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1614 AERLAYMLDDASPVALLTQANQRALLTG---DVPRILL------DTADFS-----HLSEDNPHVPGLDAHH--LAYVIYT 1677
Cdd:PRK07786  103 PPEIAFLVSDCGAHVVVTEAALAPVATAvrdIVPLLSTvvvaggSSDDSVlgyedLLAEAGPAHAPVDIPNdsPALIMYT 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1678 SGSTGKPKGVMNSHRALCNRLVWMQNTYRL-TPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMaRPDGHKDAAYLAQLI 1756
Cdd:PRK07786  183 SGTTGRPKGAVLTHANLTGQAMTCLRTNGAdINSDVGFVGVPLFHIAGIGSMLPGLLLGAPTVI-YPLGAFDPGQLLDVL 261

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1757 ERTGITTLHFVPsmlqqfVQWaDADCACDSLR------RVICSGEAlPAE--LQQRFFARF-NAQLHNLYGPTEAAiDVT 1827
Cdd:PRK07786  262 EAEKVTGIFLVP------AQW-QAVCAEQQARprdlalRVLSWGAA-PASdtLLRQMAATFpEAQILAAFGQTEMS-PVT 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1828 FWACQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlyKT 1907
Cdd:PRK07786  333 CMLLGEDAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAFAGGWF---------HS 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1908 GDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTP-DPADLR 1986
Cdd:PRK07786  404 GDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDDAAlTLEDLA 483

                         490       500       510
                  ....*....|....*....|....*....|
gi 641744967 1987 QQLGQHLAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:PRK07786  484 EFLTDRLARYKHPKALEIVDALPRNPAGKV 513
PRK07638 PRK07638
acyl-CoA synthetase; Validated
 2620-3097 1.44e-25

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 113.72  E-value: 1.44e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPdERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:PRK07638   15 PNKIAIKENDRVLTYKDWFESVCKVANWLNEKESKN-KTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERL 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQSAHLG-IMNGSLPVILLDDGEtRPFDNEPDTPLDARKQGLTPrhlAYVIYTSGSTGKPKG-VMVEHANM 2777
Cdd:PRK07638   94 AISNADMIVTERYKLNdLPDEEGRVIEIDEWK-RMIEKYLPTYAPIENVQNAP---FYMGFTSGSTGKPKAfLRAQQSWL 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2778 VNFLCSMRkEPGIAQEDVLL--G--VTSLSFDISILEIFLpllnGARLILATQAQAADAQQLamlIERHAVSFMQATPST 2853
Cdd:PRK07638  170 HSFDCNVH-DFHMKREDSVLiaGtlVHSLFLYGAISTLYV----GQTVHLMRKFIPNQVLDK---LETENISVMYTVPTM 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2854 WRMLVELRDFALPPgFKALCGGEALP----ENLATALLQkvTTLWNLYGPTETTIWSTL--NGLTTPTPYIGHPIANTQI 2927
Cdd:PRK07638  242 LESLYKENRVIENK-MKIISSGAKWEaeakEKIKNIFPY--AKLYEFYGASELSFVTALvdEESERRPNSVGRPFHNVQV 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2928 YILDAQGRVVPLGVAGEIHIAG----AGVVRGYLGRPDLTAERFITdpfsgapearmykTGDLGRWLPDGTLEYLGRNDF 3003
Cdd:PRK07638  319 RICNEAGEEVQKGEIGTVYVKSpqffMGYIIGGVLARELNADGWMT-------------VRDVGYEDEEGFIYIVGREKN 385

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3004 QVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTvlepADLRQRLSEGVAEYMIPSAFVTLDA 3083
Cdd:PRK07638  386 MILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAIIKGSATK----QQLKSFCLQRLSSFKIPKEWHFVDE 461

                         490
                  ....*....|....
gi 641744967 3084 FPLTPNGKLDRKAL 3097
Cdd:PRK07638  462 IPYTNSGKIARMEA 475
PrpE cd05967
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ...
 484-963 1.62e-25

Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.


Pssm-ID: 341271 [Multi-domain]  Cd Length: 617  Bit Score: 115.11  E-value: 1.62e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  484 EQTPEAIAVLFE------DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAY 557
Cdd:cd05967    62 AGRGDQIALIYDspvtgtERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGF 141

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  558 PAERLAYILDDAAPVALLTQSAQV----------------AQLNSTLPTVLL----DTPAAAACPDTN------------ 605
Cdd:cd05967   142 AAKELASRIDDAKPKLIVTASCGIepgkvvpykplldkalELSGHKPHHVLVlnrpQVPADLTKPGRDldwsellakaep 221

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  606 -PVVQgLHAAHLAYVIYTSGSTGRPKGV-------MVA----HRNVINLATGlhtllaldhpsrialnaSIVFDASVKNW 673
Cdd:cd05967   222 vDCVP-VAATDPLYILYTSGTTGKPKGVvrdngghAVAlnwsMRNIYGIKPG-----------------DVWWAASDVGW 283

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  674 I---------QLLSGHTLVLV---PDALrADAHQLWRYFARHAVDLFDCTPVQLQWL----LDAGLGSDPAYQPAQVL-I 736
Cdd:cd05967   284 VvghsyivygPLLHGATTVLYegkPVGT-PDPGAFWRVIEKYQVNALFTAPTAIRAIrkedPDGKYIKKYDLSSLRTLfL 362

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  737 GGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATA---CVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHI 813
Cdd:cd05967   363 AGERLDPPTLEWAENTLGVPVIDHWWQTETGWPITAnpvGLEPLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVI 442

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  814 GGAgVARGYLHRPDLTAERFIPDPFSADPAarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGV 893
Cdd:cd05967   443 KLP-LPPGCLLTLWKNDERFKKLYLSKFPG--YYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAV 519

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967  894 QDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIP-SAF---VTLDALPLTPNGKLDRKAL 963
Cdd:cd05967   520 AECAVVGVRDELKGQVPLGLVVLKEGVKITAEELEKELVALVREQIGPvAAFrlvIFVKRLPKTRSGKILRRTL 593
SgcC5_NRPS-like cd19539
SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- ...
 30-370 1.68e-25

SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- bond forming activity and similar C-domains of modular NRPSs; SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. This subfamily also includes similar C-domains of modular NRPSs such as Penicillium chrysogenum N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase PCBAB. Condensation (C) domains of NRPSs normally catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380462 [Multi-domain]  Cd Length: 427  Bit Score: 112.47  E-value: 1.68e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLHQPVQVV--WRQAPLTVN 107
Cdd:cd19539     3 PLSFAQERLWFIDQGEDGGPAYNIPGAWRLTGPLDVEALREALRDVVARHEALRTLLVRDDGGVPRQEIlpPGPAPLEVR 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  108 TLTTTSSDTVPAQLRAATDPSNHRLNLSNAPLLSATT-AHDPvcGEWLLSLSIHHLISDHITQALIIDEIRLLLEDRPEA 186
Cdd:cd19539    83 DLSDPDSDRERRLEELLRERESRGFDLDEEPPIRAVLgRFDP--DDHVLVLVAHHTAFDAWSLDVFARDLAALYAARRKG 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  187 LPKPLP-----YRNFIA---QILSVP-LSEHEQYFRNRLADIDTPTAPFDLVDVQGNGEDITEARLSLDSSLADALRRQA 257
Cdd:cd19539   161 PAAPLPelrqqYKEYAAwqrEALAAPrAAELLDFWRRRLRGAEPTALPTDRPRPAGFPYPGADLRFELDAELVAALRELA 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  258 RHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRlqGNLGADRVMGMFINTLPLRVSLRER-SVHDVVQATSHELMM 336
Cdd:cd19539   241 KRARSSLFMVLLAAYCVLLRRYTGQTDIVVGTPVAGR--NHPRFESTVGFFVNLLPLRVDVSDCaTFRDLIARVRKALVD 318

                         330       340       350
                  ....*....|....*....|....*....|....*..
gi 641744967  337 LLAHEQAPLA-LAQQCSQVPPPL--PLFSTLFNYRHS 370
Cdd:cd19539   319 AQRHQELPFQqLVAELPVDRDAGrhPLVQIVFQVTNA 355
PRK07867 PRK07867
acyl-CoA synthetase; Validated
 491-965 1.80e-25

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 114.01  E-value: 1.80e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  491 AVLFEDQHLTYRELNRRANQLAHHLIALgvQPDDR---VALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILD 567
Cdd:PRK07867   21 GLYFEDSFTSWREHIRGSAARAAALRAR--LDPTRpphVGVLLDNTPEFSLLLGAAALSGIVPVGLNPTRRGAALARDIA 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  568 DAAPVALLTQSAQVAQLNSTLPTV-LLDTPAA------AACPDTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVI 640
Cdd:PRK07867   99 HADCQLVLTESAHAELLDGLDPGVrVINVDSPawadelAAHRDAEPPFRVADPDDLFMLIFTSGTSGDPKAVRCTHRKVA 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  641 NLATGLHTLLALDhPSRIALNASIVF--DASVKNWIQLLSGHTLVlvpdALRA--DAHQLWRYFARHAVDLFDCTPVQLQ 716
Cdd:PRK07867  179 SAGVMLAQRFGLG-PDDVCYVSMPLFhsNAVMAGWAVALAAGASI----ALRRkfSASGFLPDVRRYGATYANYVGKPLS 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  717 WLLDAGLGSDPAYQPAQVLIGGEAISPAVwSRLQSLSDTRFINVYGPTEctvdaTACVVDRTQPLPT--IGKPLANTRly 794
Cdd:PRK07867  254 YVLATPERPDDADNPLRIVYGNEGAPGDI-ARFARRFGCVVVDGFGSTE-----GGVAITRTPDTPPgaLGPLPPGVA-- 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  795 ILDAQD-QPVPIGV------------TGEL-HIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLARWLPDGN 860
Cdd:PRK07867  326 IVDPDTgTECPPAEdadgrllnadeaIGELvNTAGPGGFEGYYNDPEADAERM---------RGGVYWSGDLAYRDADGY 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  861 IDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAAS--LADY 938
Cdd:PRK07867  397 AYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKFDPDAFAEFLAAQpdLGPK 476

                         490       500
                  ....*....|....*....|....*..
gi 641744967  939 MIPSAFVTLDALPLTPNGKLDRKALPA 965
Cdd:PRK07867  477 QWPSYVRVCAELPRTATFKVLKRQLSA 503
MACS_like_2 cd05973
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 2632-3097 1.89e-25

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341277 [Multi-domain]  Cd Length: 437  Bit Score: 112.61  E-value: 1.89e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQS 2711
Cdd:cd05973     1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTDA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2712 AHLGimngslpviLLDDGetrPFdnepdtpldarkqgltprhlaYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIA 2791
Cdd:cd05973    81 ANRH---------KLDSD---PF---------------------VMMFTSGTTGLPKGVPVPLRALAAFGAYLRDAVDLR 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2792 QEDVLLGVTSLSFDISIL-EIFLPLLNGARLILATQAQAADAQQLamLIERHAVSFMQATPSTWRMLVEL-RDFALPPGF 2869
Cdd:cd05973   128 PEDSFWNAADPGWAYGLYyAITGPLALGHPTILLEGGFSVESTWR--VIERLGVTNLAGSPTAYRLLMAAgAEVPARPKG 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2870 K---ALCGGEAL-PENLATALLQKVTTLWNLYGPTET-TIWSTLNGLTTPTPY--IGHPIANTQIYILDAQGRVVPLGVA 2942
Cdd:cd05973   206 RlrrVSSAGEPLtPEVIRWFDAALGVPIHDHYGQTELgMVLANHHALEHPVHAgsAGRAMPGWRVAVLDDDGDELGPGEP 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2943 GEIHIAGAGV----VRGYLGRPDltaerfitdpfsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIE 3018
Cdd:cd05973   286 GRLAIDIANSplmwFRGYQLPDT------------PAIDGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVE 353

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3019 TRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPA---DLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRK 3095
Cdd:cd05973   354 SALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPAladELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRF 433


                  ..
gi 641744967 3096 AL 3097
Cdd:cd05973   434 LL 435
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 497-963 1.95e-25

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 112.94  E-value: 1.95e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  497 QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLT 576
Cdd:cd05910     1 SRLSFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKNLKQCLQEAEPDAFIG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  577 QSaqvaqlnstlptvLLDTPAAaacpdtnpvvqglhaahlayVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPS 656
Cdd:cd05910    81 IP-------------KADEPAA--------------------ILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGE 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  657 R-------IALnasivFDASVknwiqllsGHTLVLVP-DALR---ADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGS 725
Cdd:cd05910   128 VdlatfplFAL-----FGPAL--------GLTSVIPDmDPTRparADPQKLVGAIRQYGVSIVFGSPALLERVARYCAQH 194

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  726 D-PAYQPAQVLIGGEAISPAVWSRLQSL--SDTRFINVYGPTEC---TVDATACVVDRTQPLPT------IGKPLANTRL 793
Cdd:cd05910   195 GiTLPSLRRVLSAGAPVPIALAARLRKMlsDEAEILTPYGATEAlpvSSIGSRELLATTTAATSggagtcVGRPIPGVRV 274

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  794 YILDAQDQP---------VPIGVTGELHIGGAGVARGYLHRPDLTAERFIPdpfsaDPAARI-YKTGDLARWLPDGNIDY 863
Cdd:cd05910   275 RIIEIDDEPiaewddtleLPRGEIGEITVTGPTVTPTYVNRPVATALAKID-----DNSEGFwHRMGDLGYLDDEGRLWF 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  864 LGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAReDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADY---MI 940
Cdd:cd05910   350 CGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSALVGV-GKPGCQLPVLCVEPLPGTITPRARLEQELRALAKDYphtQR 428

                         490       500
                  ....*....|....*....|....*
gi 641744967  941 PSAFVTLDALPLTP--NGKLDRKAL 963
Cdd:cd05910   429 IGRFLIHPSFPVDIrhNAKIFREKL 453
LCL_NRPS-like cd19531
LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar ...
 29-409 2.47e-25

LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar domains including the C-domain of SgcC5, a free-standing NRPS with both ester- and amide- bond forming activity; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. Streptomyces globisporus SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380454 [Multi-domain]  Cd Length: 427  Bit Score: 112.06  E-value: 2.47e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   29 YPLAPLQEGILFHYQLQEKGDTYllNSLLAFdsqtRLDAFLDV------LQQVIARHDILRTAICWQGlHQPVQVVWRQA 102
Cdd:cd19531     2 LPLSFAQQRLWFLDQLEPGSAAY--NIPGAL----RLRGPLDVaaleraLNELVARHEALRTTFVEVD-GEPVQVILPPL 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  103 PLTVNT--LTTTSSDTVPAQLRA-ATDPSNHRLNLSNAPLLSAT---TAHDpvcgEWLLSLSIHHLISDHITQALIIDEI 176
Cdd:cd19531    75 PLPLPVvdLSGLPEAEREAEAQRlAREEARRPFDLARGPLLRATllrLGED----EHVLLLTMHHIVSDGWSMGVLLREL 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  177 RLL----LEDRPEALPkPLP--YRNFIA---QILSVP-LSEHEQYFRNRLADIDTPTA-PFDLV--DVQ-GNGEditEAR 242
Cdd:cd19531   151 AALyaafLAGRPSPLP-PLPiqYADYAVwqrEWLQGEvLERQLAYWREQLAGAPPVLElPTDRPrpAVQsFRGA---RVR 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  243 LSLDSSLADALRRQARHLGISssvLFHV---AWAQVLALTSGRDDVVFGSVLSGRLQGNLgaDRVMGMFINTLPLRVSLR 319
Cdd:cd19531   227 FTLPAELTAALRALARREGAT---LFMTllaAFQVLLHRYSGQDDIVVGTPVAGRNRAEL--EGLIGFFVNTLVLRTDLS 301

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  320 ER-SVHDVVQATSHELMMLLAHEQAPL-----ALaqqcsQVPPPL---PLFSTLFNYRHSQKDASSQFWEGMRQLSGRER 390
Cdd:cd19531   302 GDpTFRELLARVRETALEAYAHQDLPFeklveAL-----QPERDLsrsPLFQVMFVLQNAPAAALELPGLTVEPLEVDSG 376

                         410       420
                  ....*....|....*....|
gi 641744967  391 T-NYPITLSVDDLGDGFNLT 409
Cdd:cd19531   377 TaKFDLTLSLTETDGGLRGS 396
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
 499-963 2.83e-25

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 113.76  E-value: 2.83e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIA-LGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVAL--- 574
Cdd:PRK12492   50 LSYAELERHSAAFAAYLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTAREMRHQFKDSGARALvyl 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  575 -----LTQS------------AQVAQLNSTLPTVLLDT--------------PAAAACPDT---------NPVVQGLHaa 614
Cdd:PRK12492  130 nmfgkLVQEvlpdtgieylieAKMGDLLPAAKGWLVNTvvdkvkkmvpayhlPQAVPFKQAlrqgrglslKPVPVGLD-- 207

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  615 HLAYVIYTSGSTGRPKGVMVAHRN-VINLATGL----------HTLLALDHPSRIA-LNASIVFDASVKNWIQLLSGHTL 682
Cdd:PRK12492  208 DIAVLQYTGGTTGLAKGAMLTHGNlVANMLQVRaclsqlgpdgQPLMKEGQEVMIApLPLYHIYAFTANCMCMMVSGNHN 287

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  683 VLV--PDALRADAHQL--WRYFARHAVD-LFdctpvqlqwlldAGLGSDPAYQPAQVLI------GGEAISPAVWSRLQS 751
Cdd:PRK12492  288 VLItnPRDIPGFIKELgkWRFSALLGLNtLF------------VALMDHPGFKDLDFSAlkltnsGGTALVKATAERWEQ 355

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  752 LSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAE 831
Cdd:PRK12492  356 LTGCTIVEGYGLTETSPVASTNPYGELARLGTVGIPVPGTALKVIDDDGNELPLGERGELCIKGPQVMKGYWQQPEATAE 435

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  832 rfipdpfsADPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLV 911
Cdd:PRK12492  436 --------ALDAEGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVK 507

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967  912 AYLCARpDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK12492  508 LFVVAR-DPGLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRREL 558
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
 1536-2021 2.94e-25

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 113.39  E-value: 2.94e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1536 DQAARTPDTTAvlFEDQH----LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:cd17642    25 KRYASVPGTIA--FTDAHtgvnYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDI 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 YPAERLAYMLDDASPVALLTQAN--QRAL-LTGDVPR----ILLDT-ADFSHLSEDN-----PHVPGLDAHH-------- 1670
Cdd:cd17642   103 YNERELDHSLNISKPTIVFCSKKglQKVLnVQKKLKIiktiIILDSkEDYKGYQCLYtfitqNLPPGFNEYDfkppsfdr 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1671 ---LAYVIYTSGSTGKPKGVMNSHRALCNRLV-WMQNTY--RLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMArpd 1744
Cdd:cd17642   183 deqVALIMNSSGSTGLPKGVQLTHKNIVARFShARDPIFgnQIIPDTAILTVIPFHHGFGMFTTLGYLICGFRVVLM--- 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1745 gHK-DAAYLAQLIERTGITTLHFVPSMLQQFVQWADAD-CACDSLRRVICSGEALPAELQQRFFARFNAQ-LHNLYGPTE 1821
Cdd:cd17642   260 -YKfEEELFLRSLQDYKVQSALLVPTLFAFFAKSTLVDkYDLSNLHEIASGGAPLSKEVGEAVAKRFKLPgIRQGYGLTE 338

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1822 --AAIDVTfwaCQPDDHRSFVPIGRPIANTQLYILDTlGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFINq 1899
Cdd:cd17642   339 ttSAILIT---PEGDDKPGAVGKVVPFFYAKVVDLDT-GKTLGPNERGELCVKGPMIMKGYVNNPEATKALIDKDGWLH- 413

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1900 pgarlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVT 1979
Cdd:cd17642   414 -------SGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLEAGKT 486

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|...
gi 641744967 1980 PDPADLRQQL-GQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd17642   487 MTEKEVMDYVaSQVSTAKRLRGGVKFVDEVPKGLTGKIDRRKI 529
ACS-like cd17634
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ...
 2607-3092 2.99e-25

acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341289 [Multi-domain]  Cd Length: 587  Bit Score: 113.83  E-value: 2.99e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2607 LIHELFEAQVACTPDAIAVVFGEA------SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAG 2680
Cdd:cd17634    54 LAANALDRHLRENGDRTAIIYEGDdtsqsrTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIG 133

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2681 GAYVPLDPTYPVERLRYMLDDAKPVALISQSAHL--GIMNGSLPV--------------ILLDDGETRPFDNEPDTPLDA 2744
Cdd:cd17634   134 AVHSVIFGGFAPEAVAGRIIDSSSRLLITADGGVraGRSVPLKKNvddalnpnvtsvehVIVLKRTGSDIDWQEGRDLWW 213

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2745 RKQ--GLTPRHLA---------YVIYTSGSTGKPKGVMVEHAN-MVNFLCSMRKEPGIAQEDVLLGVTSLSFDI--SILe 2810
Cdd:cd17634   214 RDLiaKASPEHQPeamnaedplFILYTSGTTGKPKGVLHTTGGyLVYAATTMKYVFDYGPGDIYWCTADVGWVTghSYL- 292

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2811 IFLPLLNGARLILATQAQAADAQQLAM-LIERHAVSFMQATPSTWRMLVELRDFALP----PGFKALCG-GEAL-PENLA 2883
Cdd:cd17634   293 LYGPLACGATTLLYEGVPNWPTPARMWqVVDKHGVNILYTAPTAIRALMAAGDDAIEgtdrSSLRILGSvGEPInPEAYE 372

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2884 TA---LLQKVTTLWNLYGPTET-----TIWSTLNGLTTPTPYIghPIANTQIYILDAQGRVVPLGVAGEIHIAGA--GVV 2953
Cdd:cd17634   373 WYwkkIGKEKCPVVDTWWQTETggfmiTPLPGAIELKAGSATR--PVFGVQPAVVDNEGHPQPGGTEGNLVITDPwpGQT 450

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2954 RGYLGRPDLTAERFITDpFSGapearMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVI 3033
Cdd:cd17634   451 RTLFGDHERFEQTYFST-FKG-----MYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVV 524

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967 3034 AREDSPGDKRLVAYLLAQPDtVLEPADLRQRLSEGVAEYM----IPSAFVTLDAFPLTPNGKL 3092
Cdd:cd17634   525 GIPHAIKGQAPYAYVVLNHG-VEPSPELYAELRNWVRKEIgplaTPDVVHWVDSLPKTRSGKI 586
EntF2 COG3319
Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase ...
 2609-3193 3.27e-25

Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442548 [Multi-domain]  Cd Length: 855  Bit Score: 115.19  E-value: 3.27e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2609 HELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDP 2688
Cdd:COG3319     4 AAAAAAAAAAAAAAAAAAAAAAALAAAAAAAAAAALLLLAAALLVALAALALAALALAALLAVALLAAALALAALAALAA 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2689 TYPVERLRYMLDDAKPVALISQSAHLGIMNGSLPVILLDDGETRPFDNEPDTPLDARKQGLTPRHLAYVIYTSGSTGKPK 2768
Cdd:COG3319    84 LALALAAAAAALLLAALALLLALLAALALALLALLLAALLLALAALAAAAAAAALAAAAAAAAALAAAAGLGGGGGGAGV 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2769 GVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQ 2848
Cdd:COG3319   164 LVLVLAALLALLLAALLALALALAALLLLALAAALALALLLLLALLLLLLLLLALLLLLLLALLAAAALLALLLALLLLL 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2849 ATPSTWRMLVELRDFALPPGFKALCGGEA-----LPENLATALLQKVTTLWNLYGPTETTIWSTLNGLTTPTPYIGHPIA 2923
Cdd:COG3319   244 LAALLLLLALALLLLLALLLLLGLLALLLallllLALLLLAAAAALAAGGTATTAAVTTTAAAAAPGVAGALGPIGGGPG 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2924 NTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGAPEARMYKTGDLGRWLPDGTLEYLGRNDF 3003
Cdd:COG3319   324 LLVLLVLLVLLLPLLLGVGGGGGGGGGGGGAGGLAGRGLRAAAALRDPAGAGARGRLRRGGDRGRRLGGGLLLGLGRLRL 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3004 QVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDkRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDA 3083
Cdd:COG3319   404 QRLRRGLREELEEAEAALAEAAAVAAAVAAAAAAAAAA-AALAAAVVAAAALAAAALLLLLLLLLLPPPLPPALLLLLLL 482

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3084 FPLTPNGKLDRKALPAPDQSAMATRgyEAPQGDLEHALAQIWQTLLGVERVGRHDHFFELGGHSLLAVQLNARIRAEFLT 3163
Cdd:COG3319   483 LLLLLLAALLLAAAAPAAAAAAAAA--PAPAAALELALALLLLLLLGLGLVGDDDDFFGGGGGSLLALLLLLLLLALLLR 560

                         570       580       590
                  ....*....|....*....|....*....|
gi 641744967 3164 DIPIVAIFQHPQLSALAEVILAAQIHAAWG 3193
Cdd:COG3319   561 LLLLLALLLAPTLAALAAALAAAAAAAALS 590
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
 495-963 3.45e-25

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 113.15  E-value: 3.45e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  495 EDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPA----YPAERLAYI----- 565
Cdd:cd05906    36 SEEFQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLTVPptydEPNARLRKLrhiwq 115

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  566 -LDDaaPVaLLTQSAQVAQLN-----STLPTVLLDTPAAAACPDTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNV 639
Cdd:cd05906   116 lLGS--PV-VLTDAELVAEFAgletlSGLPGIRVLSIEELLDTAADHDLPQSRPDDLALLMLTSGSTGFPKAVPLTHRNI 192

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  640 INLATGLHTL------------LALDHPsrialnASIVFD--ASVKNWIQLLSGHTLVLVPDALR----ADAHQLWRYFA 701
Cdd:cd05906   193 LARSAGKIQHngltpqdvflnwVPLDHV------GGLVELhlRAVYLGCQQVHVPTEEILADPLRwldlIDRYRVTITWA 266

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  702 -----RHAVDLFdCTPVQLQWLLDAGLgsdpayqpaQVLIGGEAISPAVWSRLQS------LSDTRFINVYGPTECTVDA 770
Cdd:cd05906   267 pnfafALLNDLL-EEIEDGTWDLSSLR---------YLVNAGEAVVAKTIRRLLRllepygLPPDAIRPAFGMTETCSGV 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  771 TACVVDRTQPLPT------IGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaa 844
Cdd:cd05906   337 IYSRSFPTYDHSQalefvsLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPEANAEAFTEDGW------ 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  845 riYKTGDLArWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQD--AVVIAREDSPGDTRLVAYLCArPDAEL 922
Cdd:cd05906   411 --FRTGDLG-FLDNGNLTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPsfTAAFAVRDPGAETEELAIFFV-PEYDL 486

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 641744967  923 HPA------ALRQQLAASL---ADYMIPsafVTLDALPLTPNGKLDRKAL 963
Cdd:cd05906   487 QDAlsetlrAIRSVVSREVgvsPAYLIP---LPKEEIPKTSLGKIQRSKL 533
PRK06145 PRK06145
acyl-CoA synthetase; Validated
 2619-3097 4.25e-25

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 112.29  E-value: 4.25e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:PRK06145   15 TPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEVAYI 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISQSAHLGIMNGSLPVILLD-----DGETRPFDNEPDTPLDARKqgltPRHLAYVIYTSGSTGKPKGVMVE 2773
Cdd:PRK06145   95 LGDAGAKLLLVDEEFDAIVALETPKIVIDaaaqaDSRRLAQGGLEIPPQAAVA----PTDLVRLMYTSGTTDRPKGVMHS 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2774 HANMVNFLCSMRKEPGIAQEDVLLGVTSL----SFDISILEIflpLLNGARLILATQAQAADAQQLamlIERHAVSFMQA 2849
Cdd:PRK06145  171 YGNLHWKSIDHVIALGLTASERLLVVGPLyhvgAFDLPGIAV---LWVGGTLRIHREFDPEAVLAA---IERHRLTCAWM 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2850 TP-STWRML-VELRD-FALPPGFKALCGGEALPENLATALLQKVTT--LWNLYGPTETTIWSTLNGLTTPTPYIG---HP 2921
Cdd:PRK06145  245 APvMLSRVLtVPDRDrFDLDSLAWCIGGGEKTPESRIRDFTRVFTRarYIDAYGLTETCSGDTLMEAGREIEKIGstgRA 324

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2922 IANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmyKTGDLGRWLPDGTLEYLGRN 3001
Cdd:PRK06145  325 LAHVEIRIADGAGRWLPPNMKGEICMRGPKVTKGYWKDPEKTAEAFYGDWF---------RSGDVGYLDEEGFLYLTDRK 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3002 DFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTL 3081
Cdd:PRK06145  396 KDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATLTLEALDRHCRQRLASFKVPRQLKVR 475

                         490
                  ....*....|....*.
gi 641744967 3082 DAFPLTPNGKLDRKAL 3097
Cdd:PRK06145  476 DELPRNPSGKVLKRVL 491
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
 1674-2016 4.69e-25

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 109.13  E-value: 4.69e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1674 VIYTSGSTGKPKGVMNSHR-ALCNRLVWMQNTyRLTPDDRVLQKTPF--SFDVSVwEFFWPLLYGARLVmarPDGHKDAA 1750
Cdd:cd17638     5 IMFTSGTTGRSKGVMCAHRqTLRAAAAWADCA-DLTEDDRYLIINPFfhTFGYKA-GIVACLLTGATVV---PVAVFDVD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1751 YLAQLIERTGITTLHFVPSMLQQFVQWADADCA-CDSLRRVICSGEALPAELQQRFFAR--FNAQLhNLYGPTEAaidVT 1827
Cdd:cd17638    80 AILEAIERERITVLPGPPTLFQSLLDHPGRKKFdLSSLRAAVTGAATVPVELVRRMRSElgFETVL-TAYGLTEA---GV 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1828 FWACQPDDHRSFVP--IGRPIANTQLYILDtlgqpvplgvAGELHIGGVGVARGYLNRPDLTAERFIPDPFINqpgarly 1905
Cdd:cd17638   156 ATMCRPGDDAETVAttCGRACPGFEVRIAD----------DGEVLVRGYNVMQGYLDDPEATAEAIDADGWLH------- 218

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1906 kTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADL 1985
Cdd:cd17638   219 -TGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGVTLTEEDV 297

                         330       340       350
                  ....*....|....*....|....*....|.
gi 641744967 1986 RQQLGQHLAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:cd17638   298 IAWCRERLANYKVPRFVRFLDELPRNASGKV 328
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1671-2021 5.46e-25

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 109.49  E-value: 5.46e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1671 LAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTP-FSFDVSVWEFFWPLLYGARLVMARPDGHKDA 1749
Cdd:cd05944     4 VAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPlFHVNGSVVTLLTPLASGAHVVLAGPAGYRNP 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1750 AY---LAQLIERTGITTLHFVPSMLQQFVQW-ADADCAcdSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAID 1825
Cdd:cd05944    84 GLfdnFWKLVERYRITSLSTVPTVYAALLQVpVNADIS--SLRFAMSGAAPLPVELRARFEDATGLPVVEGYGLTEATCL 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1826 VTfwACQPDDHRSFVPIGRPIANTQLYI--LDTLGQ---PVPLGVAGELHIGGVGVARGYLNRpDLTAERFIPDPFINqp 1900
Cdd:cd05944   162 VA--VNPPDGPKRPGSVGLRLPYARVRIkvLDGVGRllrDCAPDEVGEICVAGPGVFGGYLYT-EGNKNAFVADGWLN-- 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1901 garlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTP 1980
Cdd:cd05944   237 ------TGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVV 310

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 641744967 1981 DPADLRQQLGQHLAEY-MVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05944   311 EEEELLAWARDHVPERaAVPKHIEVLEELPVTAVGKVFKPAL 352
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
2618-3097 5.74e-25

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 112.39  E-value: 5.74e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2618 CTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGgaYVPLDPTYPVER--L 2695
Cdd:PRK10946   35 AASDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLG--VAPVNALFSHQRseL 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2696 RYMLDDAKPVALISQSAH-----------LGIMNGSLPVILL-DDGETRPFDNEPDTPLDARKQGLTPR-HLAYVIYTSG 2762
Cdd:PRK10946  113 NAYASQIEPALLIADRQHalfsdddflntLVAEHSSLRVVLLlNDDGEHSLDDAINHPAEDFTATPSPAdEVAFFQLSGG 192

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2763 STGKPKGVMVEHANM----------------VNFLCS-------MRKEPGIaqedvlLGVtslsfdisileiflpLLNGA 2819
Cdd:PRK10946  193 STGTPKLIPRTHNDYyysvrrsveicgftpqTRYLCAlpaahnyPMSSPGA------LGV---------------FLAGG 251

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2820 RLILATQAQAADAQQlamLIERHAVSFMQATP---STWrmlveLRDFALPPGFKAL-------CGGEALPENLATAL--- 2886
Cdd:PRK10946  252 TVVLAPDPSATLCFP---LIEKHQVNVTALVPpavSLW-----LQAIAEGGSRAQLaslkllqVGGARLSETLARRIpae 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2887 ----LQKVttlwnlYGPTETTIWSTlnGLTTPTPYI----GHPIA-NTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYL 2957
Cdd:PRK10946  324 lgcqLQQV------FGMAEGLVNYT--RLDDSDERIfttqGRPMSpDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYY 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2958 GRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIARED 3037
Cdd:PRK10946  396 KSPQHNASAFDANGF--------YCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMED 467

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967 3038 SPGDKRLVAYLLAQPDtvLEPADLRQRLSE-GVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK10946  468 ELMGEKSCAFLVVKEP--LKAVQLRRFLREqGIAEFKLPDRVECVDSLPLTAVGKVDKKQL 526
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
 2624-3097 5.79e-25

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 112.08  E-value: 5.79e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2624 AVVF----GEAS-LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYM 2698
Cdd:PRK08008   25 ALIFessgGVVRrYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESAWI 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISQSAHLGIMNGSLP--------VILLDDGE---------TRPFDNEPDTPLDARKqgLTPRHLAYVIYTS 2761
Cdd:PRK08008  105 LQNSQASLLVTSAQFYPMYRQIQQedatplrhICLTRVALpaddgvssfTQLKAQQPATLCYAPP--LSTDDTAEILFTS 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2762 GSTGKPKGVMVEHANMV------NFLCSMRKEpgiaqeDVLLGVTSlSFDISI-LEIFLPLLN-GARLILA--------- 2824
Cdd:PRK08008  183 GTTSRPKGVVITHYNLRfagyysAWQCALRDD------DVYLTVMP-AFHIDCqCTAAMAAFSaGATFVLLekysarafw 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2825 ---TQAQAADAQQLAMLIErhavSFMQATPSTWRMLVELRD--FALPpgfkalcggeaLPENLATALLQKV-TTLWNLYG 2898
Cdd:PRK08008  256 gqvCKYRATITECIPMMIR----TLMVQPPSANDRQHCLREvmFYLN-----------LSDQEKDAFEERFgVRLLTSYG 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2899 PTETTIWstLNGlTTPT-----PYIGHPIANTQIYILDAQGRVVPLGVAGEIHI---AGAGVVRGYLGRPDLTAERFitd 2970
Cdd:PRK08008  321 MTETIVG--IIG-DRPGdkrrwPSIGRPGFCYEAEIRDDHNRPLPAGEIGEICIkgvPGKTIFKEYYLDPKATAKVL--- 394

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2971 pfsgAPEARMYkTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLA 3050
Cdd:PRK08008  395 ----EADGWLH-TGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVL 469

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*..
gi 641744967 3051 QPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK08008  470 NEGETLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
499-963 8.59e-25

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 112.28  E-value: 8.59e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIA-LGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLT- 576
Cdd:PRK08751   51 ITYREADQLVEQFAAYLLGeLQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVi 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  577 -----------QSAQVAQLNSTLPTVLLDTPAAAA-----------CPD-------------------TNPVVQgLHAAH 615
Cdd:PRK08751  131 dnfgttvqqviADTPVKQVITTGLGDMLGFPKAALvnfvvkyvkklVPEyringairfrealalgrkhSMPTLQ-IEPDD 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  616 LAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDhpSRIALNASIVFDASVKNWIQLLSGHTLVLV---------- 685
Cdd:PRK08751  210 IAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQWLAGT--GKLEEGCEVVITALPLYHIFALTANGLVFMkiggcnhlis 287

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  686 -PDALRADAHQL--WRYFARHAVD-LFDctpvqlqwlldaGLGSDPAYQPAQ------VLIGGEAISPAVWSRLQSLSDT 755
Cdd:PRK08751  288 nPRDMPGFVKELkkTRFTAFTGVNtLFN------------GLLNTPGFDQIDfsslkmTLGGGMAVQRSVAERWKQVTGL 355

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  756 RFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIP 835
Cdd:PRK08751  356 TLVEAYGLTETSPAACINPLTLKEYNGSIGLPIPSTDACIKDDAGTVLAIGEIGELCIKGPQVMKGYWKRPEETAKVMDA 435

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  836 DPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDtRLVAYLC 915
Cdd:PRK08751  436 DGW--------LHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVPDEKSG-EIVKVVI 506

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 641744967  916 ARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK08751  507 VKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRREL 554
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
 480-963 1.04e-24

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 111.82  E-value: 1.04e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  480 EAQAEQTPEAIAVLFEDQH-----LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMD 554
Cdd:cd05970    24 DAMAKEYPDKLALVWCDDAgeeriFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPAT 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  555 PAYPAERLAYILDDA---APVAL----LTQSAQVAQLNSTLPTVLL----DTPAA-----AACPDTNPVVQGLHAA---- 614
Cdd:cd05970   104 HQLTAKDIVYRIESAdikMIVAIaednIPEEIEKAAPECPSKPKLVwvgdPVPEGwidfrKLIKNASPDFERPTANsypc 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  615 --HLAYVIYTSGSTGRPKgvMVAHRNVINLAtglHTLLA-----LDHPSRIALNASIVFDASV--KNWIQLLSGhTLVLV 685
Cdd:cd05970   184 geDILLVYFSSGTTGMPK--MVEHDFTYPLG---HIVTAkywqnVREGGLHLTVADTGWGKAVwgKIYGQWIAG-AAVFV 257

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  686 PDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTE 765
Cdd:cd05970   258 YDYDKFDPKALLEKLSKYGVTTFCAPPTIYRFLIREDLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIKLMEGFGQTE 337

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  766 CTVdaTACVVDRTQPLP-TIGKPLANTRLYILDAQDQPVPIGVTGELHIGGA-----GVARGYLHRPDLTAERFIPDpfs 839
Cdd:cd05970   338 TTL--TIATFPWMEPKPgSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIRTSkgkpvGLFGGYYKDAEKTAEVWHDG--- 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  840 adpaarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPD 919
Cdd:cd05970   413 ------YYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLAKG 486

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967  920 AElhPA-ALRQQLAASL----ADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05970   487 YE--PSeELKKELQDHVkkvtAPYKYPRIVEFVDELPKTISGKIRRVEI 533
Ac_CoA_lig_AcsA TIGR02188
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ...
 482-963 1.11e-24

acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.


Pssm-ID: 274022 [Multi-domain]  Cd Length: 626  Bit Score: 112.34  E-value: 1.11e-24
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   482 QAEQTPEAIAVLFE------DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDP 555
Cdd:TIGR02188   66 HLEARPDKVAIIWEgdepgeVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFG 145

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   556 AYPAERLAYILDDAAPVALLTQSAQVAQlNSTLPTVLLDTPAAAACPDT-----------NPV---VQG----------- 610
Cdd:TIGR02188  146 GFSAEALADRINDAGAKLVITADEGLRG-GKVIPLKAIVDEALEKCPVSvehvlvvrrtgNPVvpwVEGrdvwwhdlmak 224

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   611 ---------LHAAHLAYVIYTSGSTGRPKGVMvaHrnvinlATGLHTLLAldhpsriALNASIVFD----------ASVk 671
Cdd:TIGR02188  225 asaycepepMDSEDPLFILYTSGSTGKPKGVL--H------TTGGYLLYA-------AMTMKYVFDikdgdifwctADV- 288

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   672 NWIqllSGHT-LVLVPDALRA------------DAHQLWRYFARHAVDLFDCTPVQLQWLLDAG--------------LG 724
Cdd:TIGR02188  289 GWI---TGHSyIVYGPLANGAttvmfegvptypDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGdewvkkhdlsslrlLG 365

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   725 SdpayqpaqVligGEAISPAVWSrlqslsdtRFINVYGPTECT-VDA-----TACVVdrTQPLP--TIGKPLANTRLY-- 794
Cdd:TIGR02188  366 S--------V---GEPINPEAWM--------WYYKVVGKERCPiVDTwwqteTGGIM--ITPLPgaTPTKPGSATLPFfg 424

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   795 ----ILDAQDQPVPI-GVTGELHIGGA--GVARGYLHRPdltaERFIPDPFSadPAARIYKTGDLARWLPDGNIDYLGRN 867
Cdd:TIGR02188  425 iepaVVDEEGNPVEGpGEGGYLVIKQPwpGMLRTIYGDH----ERFVDTYFS--PFPGYYFTGDGARRDKDGYIWITGRV 498

                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   868 DFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHP---AALRQQLAASLADYMIPSAF 944
Cdd:TIGR02188  499 DDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDDelrKELRKHVRKEIGPIAKPDKI 578

                          570
                   ....*....|....*....
gi 641744967   945 VTLDALPLTPNGKLDRKAL 963
Cdd:TIGR02188  579 RFVPGLPKTRSGKIMRRLL 597
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
 483-963 2.84e-24

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 110.17  E-value: 2.84e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLF--EDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAE 560
Cdd:PRK13391    7 AQTTPDKPAVIMasTGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPA 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  561 RLAYILDDAAPVALLTQSAQ---VAQLNSTLPTVLLD---------------TPAAAACPDTNPVVQGLHAAHLayviYT 622
Cdd:PRK13391   87 EAAYIVDDSGARALITSAAKldvARALLKQCPGVRHRlvldgdgelegfvgyAEAVAGLPATPIADESLGTDML----YS 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  623 SGSTGRPKGVM--VAHRNV---INLATGLHTLLALDHPSrIALNASIVFDASVKNW---IQLLSGHTLVLVpdalRADAH 694
Cdd:PRK13391  163 SGTTGRPKGIKrpLPEQPPdtpLPLTAFLQRLWGFRSDM-VYLSPAPLYHSAPQRAvmlVIRLGGTVIVME----HFDAE 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  695 QLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPA--QVLIGGEAISPAVWSRlqslsdtRFINVYGPT-----ECT 767
Cdd:PRK13391  238 QYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKYDLSslEVAIHAAAPCPPQVKE-------QMIDWWGPIiheyyAAT 310

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  768 VDATACVVDRTQPLP---TIGKPLANTrLYILDAQDQPVPIGVTGELHIGGaGVARGYLHRPDLTAErfipdpfSADPAA 844
Cdd:PRK13391  311 EGLGFTACDSEEWLAhpgTVGRAMFGD-LHILDDDGAELPPGEPGTIWFEG-GRPFEYLNDPAKTAE-------ARHPDG 381

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  845 RIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIA--REDSPGDTRLVAYLC--ARPDA 920
Cdd:PRK13391  382 TWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGvpNEDLGEEVKAVVQPVdgVDPGP 461

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|...
gi 641744967  921 ELhPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK13391  462 AL-AAELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLL 503
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
 1674-2018 3.66e-24

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 106.58  E-value: 3.66e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1674 VIYTSGSTGKPKGVMNSHRALCNRLV-WMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPdgHKDAAYL 1752
Cdd:cd17635     6 VIFTSGTTGEPKAVLLANKTFFAVPDiLQKEGLNWVVGDVTYLPLPATHIGGLWWILTCLIHGGLCVTGGE--NTTYKSL 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1753 AQLIERTGITTLHFVPSMLQQFV-QWADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEaaidVTFWAC 1831
Cdd:cd17635    84 FKILTTNAVTTTCLVPTLLSKLVsELKSANATVPSLRLIGYGGSRAIAADVRFIEATGLTNTAQVYGLSE----TGTALC 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1832 QP--DDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlyKTGD 1909
Cdd:cd17635   160 LPtdDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTAEVLIDGWV---------NTGD 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1910 LARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA-DLRQQ 1988
Cdd:cd17635   231 LGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASAELDENAIrALKHT 310

                         330       340       350
                  ....*....|....*....|....*....|
gi 641744967 1989 LGQHLAEYMVPGAFVTLDAFPLTPNGKLDR 2018
Cdd:cd17635   311 IRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
PRK08162 PRK08162
acyl-CoA synthetase; Validated
 483-963 4.64e-24

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 109.65  E-value: 4.64e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERL 562
Cdd:PRK08162   28 AEVYPDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASI 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  563 AYILDDAAPVALLTQS--AQVAQlnstlptvlldtPAAAACPDTNPVV---------QGLHAAHLAY------------- 618
Cdd:PRK08162  108 AFMLRHGEAKVLIVDTefAEVAR------------EALALLPGPKPLVidvddpeypGGRFIGALDYeaflasgdpdfaw 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  619 -----------VIYTSGSTGRPKGVMVAHR--------NVINLATGLHTL----LALDH------PSRIALNASI-VFda 668
Cdd:PRK08162  176 tlpadewdaiaLNYTSGTTGNPKGVVYHHRgaylnalsNILAWGMPKHPVylwtLPMFHcngwcfPWTVAARAGTnVC-- 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  669 svknwiqllsghtlvlvpdaLR-ADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAY-QPAQVLIGGEAISPAVW 746
Cdd:PRK08162  254 --------------------LRkVDPKLIFDLIREHGVTHYCGAPIVLSALINAPAEWRAGIdHPVHAMVAGAAPPAAVI 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  747 SRLQSLSdTRFINVYGPTECTVDATACVvdrTQP----LPTIGKPLANTR----------LYILDAQD-QPVPI-GVT-G 809
Cdd:PRK08162  314 AKMEEIG-FDLTHVYGLTETYGPATVCA---WQPewdaLPLDERAQLKARqgvryplqegVTVLDPDTmQPVPAdGETiG 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  810 ELHIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLARWLPDGNIdylgrndfQIKVR--------GFRIEAG 881
Cdd:PRK08162  390 EIMFRGNIVMKGYLKNPKATEEAF---------AGGWFHTGDLAVLHPDGYI--------KIKDRskdiiisgGENISSI 452

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  882 EIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAfVTLDALPLTPNGKLDRK 961
Cdd:PRK08162  453 EVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEEEIIAHCREHLAGFKVPKA-VVFGELPKTSTGKIQKF 531


                  ..
gi 641744967  962 AL 963
Cdd:PRK08162  532 VL 533
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
 619-959 5.02e-24

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 106.23  E-value: 5.02e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  619 VIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSrIALNASIVFDASVKNWI--QLLSGHTLVLVPdalRADAHQL 696
Cdd:cd17636     5 AIYTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGT-VFLNSGPLFHIGTLMFTlaTFHAGGTNVFVR---RVDAEEV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  697 WRYFARHAVDL-FDCTPVQLQWL-LDAGLGSDpayqpaqvLIGGEAISPA-VWSRLQSLSDTRFINV---YGPTECTVDA 770
Cdd:cd17636    81 LELIEAERCTHaFLLPPTIDQIVeLNADGLYD--------LSSLRSSPAApEWNDMATVDTSPWGRKpggYGQTEVMGLA 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  771 TACVVDRtQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTG 850
Cdd:cd17636   153 TFAALGG-GAIGGAGRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRT---------RGGWHHTN 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  851 DLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQ 930
Cdd:cd17636   223 DLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGASVTEAELIEH 302

                         330       340
                  ....*....|....*....|....*....
gi 641744967  931 LAASLADYMIPSAFVTLDALPLTPNGKLD 959
Cdd:cd17636   303 CRARIASYKKPKSVEFADALPRTAGGADD 331
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
 2616-3097 5.09e-24

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 109.40  E-value: 5.09e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2616 VACTPDAIAVVFGE--ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVE 2693
Cdd:PRK13391    7 AQTTPDKPAVIMAStgEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPA 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2694 RLRYMLDDAKPVALISQSAHLGIM--------NGSLPVILLDDGETRPFDN-------EPDTPLDARKQGltprhlAYVI 2758
Cdd:PRK13391   87 EAAYIVDDSGARALITSAAKLDVArallkqcpGVRHRLVLDGDGELEGFVGyaeavagLPATPIADESLG------TDML 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2759 YTSGSTGKPKGVMVEHAnmvnflcsmrkEPGIAQEDVLLGVTSLSFDISILEIFL---PLLNGARLILATQAQAADAQQL 2835
Cdd:PRK13391  161 YSSGTTGRPKGIKRPLP-----------EQPPDTPLPLTAFLQRLWGFRSDMVYLspaPLYHSAPQRAVMLVIRLGGTVI 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2836 AM----------LIERHAVSFMQATPSTW-RMLV---ELRD-FALPPGFKALCGGEALPENLATALLQKV-TTLWNLYGP 2899
Cdd:PRK13391  230 VMehfdaeqylaLIEEYGVTHTQLVPTMFsRMLKlpeEVRDkYDLSSLEVAIHAAAPCPPQVKEQMIDWWgPIIHEYYAA 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2900 TE---TTIWSTLNGLTTPTPyIGHPIANTqIYILDAQGRVVPLGVAGEIHIAGAGVVRgYLGRPDLTAErfitdpfSGAP 2976
Cdd:PRK13391  310 TEglgFTACDSEEWLAHPGT-VGRAMFGD-LHILDDDGAELPPGEPGTIWFEGGRPFE-YLNDPAKTAE-------ARHP 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2977 EARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIA--REDSPGDKRLVAYLL--AQP 3052
Cdd:PRK13391  380 DGTWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGvpNEDLGEEVKAVVQPVdgVDP 459

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*
gi 641744967 3053 DTVLEpADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK13391  460 GPALA-AELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLL 503
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
 495-963 8.96e-24

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 108.19  E-value: 8.96e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  495 EDQH---LTYRELnRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAP 571
Cdd:cd05909     1 EDTLgtsLTYRKL-LTGAIALARKLAKMTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  572 VALLTQSAQVAQLNST-LPTVLLD--------------------TPAAAACPDTNPVVQ-GLH---AAHLAYVIYTSGST 626
Cdd:cd05909    80 KTVLTSKQFIEKLKLHhLFDVEYDarivyledlrakiskadkckAFLAGKFPPKWLLRIfGVApvqPDDPAVILFTSGSE 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  627 GRPKGVMVAHRNVINLATGLHTLLALDhPSRIALNASIVFDA---SVKNWIQLLSGHTLVLVPDALraDAHQLWRYFARH 703
Cdd:cd05909   160 GLPKGVVLSHKNLLANVEQITAIFDPN-PEDVVFGALPFFHSfglTGCLWLPLLSGIKVVFHPNPL--DYKKIPELIYDK 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  704 AVDLFDCTPVqlqwLLDAGLGSDPAYQPAQ---VLIGGEAISPAVWSRLQSLSDTRFINVYGPTECtvdatACVVDRTQP 780
Cdd:cd05909   237 KATILLGTPT----FLRGYARAAHPEDFSSlrlVVAGAEKLKDTLRQEFQEKFGIRILEGYGTTEC-----SPVISVNTP 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  781 LP-----TIGKPLANTRLYILDAQD-QPVPIGVTGELHIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLAR 854
Cdd:cd05909   308 QSpnkegTVGRPLPGMEVKIVSVEThEEVPIGEGGLLLVRGPNVMLGYLNEPELTSFAF---------GDGWYDTGDIGK 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  855 WLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLL-RCPG-VQDAVVIAREDSPGDtRLVAyLCARPDAElhPAALRQQL- 931
Cdd:cd05909   379 IDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSeILPEdNEVAVVSVPDGRKGE-KIVL-LTTTTDTD--PSSLNDILk 454

                         490       500       510
                  ....*....|....*....|....*....|..
gi 641744967  932 AASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05909   455 NAGISNLAKPSYIHQVEEIPLLGTGKPDYVTL 486
PRK08315 PRK08315
AMP-binding domain protein; Validated
 2599-3091 1.41e-23

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 108.36  E-value: 1.41e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2599 DADIP-RHALIHELFEAQVACTPDAIAVVFGEASL--SYDELNRRANRLAHHLISFGVRPDERVAI----CVErgldMVV 2671
Cdd:PRK08315    8 PTDVPlLEQTIGQLLDRTAARYPDREALVYRDQGLrwTYREFNEEVDALAKGLLALGIEKGDRVGIwapnVPE----WVL 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2672 GLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALIS-----QSAHLGIMNGSLP--------------------VILL 2726
Cdd:PRK08315   84 TQFATAKIGAILVTINPAYRLSELEYALNQSGCKALIAadgfkDSDYVAMLYELAPelatcepgqlqsarlpelrrVIFL 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2727 DDGETRPFDN----------EPDTPLDARKQGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVN--FLCSMRKepGIAQED 2794
Cdd:PRK08315  164 GDEKHPGMLNfdellalgraVDDAELAARQATLDPDDPINIQYTSGTTGFPKGATLTHRNILNngYFIGEAM--KLTEED 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2795 -----------------VLLGVTSLSFDISILEIFLPLlngarLILATQAQaadaqqlamliER----HAVSFMqatpst 2853
Cdd:PRK08315  242 rlcipvplyhcfgmvlgNLACVTHGATMVYPGEGFDPL-----ATLAAVEE-----------ERctalYGVPTM------ 299

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2854 wrMLVEL--RDFA------LPPGFKAlcgGEALPEnlatALLQKVTTLWNL------YGPTETTIWSTLNGLTTP----T 2915
Cdd:PRK08315  300 --FIAELdhPDFArfdlssLRTGIMA---GSPCPI----EVMKRVIDKMHMsevtiaYGMTETSPVSTQTRTDDPlekrV 370

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2916 PYIGHPIANTQIYILD-AQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearMYkTGDLGRWLPDGT 2994
Cdd:PRK08315  371 TTVGRALPHLEVKIVDpETGETVPRGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGW-------MH-TGDLAVMDEEGY 442

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2995 LEYLGRndfqVK---VRGfrielG------EIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRL 3065
Cdd:PRK08315  443 VNIVGR----IKdmiIRG-----GeniyprEIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEEDVRDFC 513

                         570       580
                  ....*....|....*....|....*.
gi 641744967 3066 SEGVAEYMIPSAFVTLDAFPLTPNGK 3091
Cdd:PRK08315  514 RGKIAHYKIPRYIRFVDEFPMTVTGK 539
E-C_NRPS cd19544
Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); ...
 1090-1399 1.53e-23

Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); Dual function Epimerization/Condensation (E/C) domains have both an epimerization and a DCL condensation activity. Dual E/C domains first epimerize the substrate amino acid to produce a D-configuration, then catalyze the condensation between the D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. They are D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. These Dual E/C domains contain an extended His-motif (HHx(N)GD) near the N-terminus of the domain in addition to the standard Condensation (C) domain active site motif (HHxxxD). C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains, these include the DCL-type, LCL-type, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C domains, and the X-domain.


Pssm-ID: 380466 [Multi-domain]  Cd Length: 413  Bit Score: 106.37  E-value: 1.53e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1090 AYHLPAALHLTGR--LDRpaLTTALNGLVARHESLRTRFTSiDG--QPAQQIdpdtlgfslsshdLRK-------LDEAA 1158
Cdd:cd19544    23 PYLLRSLLAFDSRarLDA--FLAALQQVIDRHDILRTAILW-EGlsEPVQVV-------------WRQaelpveeLTLDP 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1159 RTTRVAELAE--QEARARFDLTQGPLIRGQLLQLDDN-THVLLLTQHHIISDGWSIGILARELAALyqaaLEGSEANLPP 1235
Cdd:cd19544    87 GDDALAQLRArfDPRRYRLDLRQAPLLRAHVAEDPANgRWLLLLLFHHLISDHTSLELLLEEIQAI----LAGRAAALPP 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1236 lPVQYADYAVwqrQWLQGETLNDLRDYWRDQLQG-----AP-ALLEIPTDRPRPSVQRYAgdqvpfhLDAGQLRRLHALN 1309
Cdd:cd19544   163 -PVPYRNFVA---QARLGASQAEHEAFFREMLGDvdeptAPfGLLDVQGDGSDITEARLA-------LDAELAQRLRAQA 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1310 RQQGT---TLFMtllAAWSVVLSRLSGQDDIVIGTPVANRpRQELEG---MVGFFVNTLALRTEPGRChAVADLLDQVRE 1383
Cdd:cd19544   232 RRLGVspaSLFH---LAWALVLARCSGRDDVVFGTVLSGR-MQGGAGadrALGMFINTLPLRVRLGGR-SVREAVRQTHA 306

                         330
                  ....*....|....*...
gi 641744967 1384 R--ALdayahqaLPFEQV 1399
Cdd:cd19544   307 RlaEL-------LRHEHA 317
PRK07867 PRK07867
acyl-CoA synthetase; Validated
 1528-2023 1.58e-23

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 107.84  E-value: 1.58e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1528 ALIHQLVEDqaartpDTTAVLFEDQHLTYDALNRRANQLAHHLIDLgvKPDDR---IAICVERSLDMVIGLLAILKAGAA 1604
Cdd:PRK07867    9 ELLLPLAED------DDRGLYFEDSFTSWREHIRGSAARAAALRAR--LDPTRpphVGVLLDNTPEFSLLLGAAALSGIV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1605 YVPLDPGYPAERLAYMLDDASPVALLTQANQRALLTGD---VPRILLDTADFSHLSEDNPHVP----GLDAHHLAYVIYT 1677
Cdd:PRK07867   81 PVGLNPTRRGAALARDIAHADCQLVLTESAHAELLDGLdpgVRVINVDSPAWADELAAHRDAEppfrVADPDDLFMLIFT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1678 SGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLY-GARLVMAR--------PDghkd 1748
Cdd:PRK07867  161 SGTSGDPKAVRCTHRKVASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVALAaGASIALRRkfsasgflPD---- 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1749 aaylaqlIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSGEALPAELQqRFFARFNAQLHNLYGPTEAAIDVTF 1828
Cdd:PRK07867  237 -------VRRYGATYANYVGKPLSYVLATPERPDDADNPLRIVYGNEGAPGDIA-RFARRFGCVVVDGFGSTEGGVAITR 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1829 WACQPDDhrsfvPIGRPIANTQLYILDTlGQPVPLGVA------------GEL-HIGGVGVARGYLNRPDLTAERFipdp 1895
Cdd:PRK07867  309 TPDTPPG-----ALGPLPPGVAIVDPDT-GTECPPAEDadgrllnadeaiGELvNTAGPGGFEGYYNDPEADAERM---- 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1896 finqPGARlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQ 1975
Cdd:PRK07867  379 ----RGGV-YWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLA 453

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 641744967 1976 PGVTPDPADLRQQLGQH--LAEYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK07867  454 PGAKFDPDAFAEFLAAQpdLGPKQWPSYVRVCAELPRTATFKVLKRQLSA 503
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
 1533-2021 2.02e-23

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 107.58  E-value: 2.02e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1533 LVEDQAARTPDTTAVLFEDQH-----LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVP 1607
Cdd:cd05970    22 VVDAMAKEYPDKLALVWCDDAgeeriFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIP 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1608 LDPGYPAERLAYMLDDASPVALLTQANQ------------------RALLTGDVP-------RILLDTADFSHLSEDNPH 1662
Cdd:cd05970   102 ATHQLTAKDIVYRIESADIKMIVAIAEDnipeeiekaapecpskpkLVWVGDPVPegwidfrKLIKNASPDFERPTANSY 181

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1663 VPGLDahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVW-EFFWPLLYGARlVMA 1741
Cdd:cd05970   182 PCGED---ILLVYFSSGTTGMPKMVEHDFTYPLGHIVTAKYWQNVREGGLHLTVADTGWGKAVWgKIYGQWIAGAA-VFV 257

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1742 RPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTE 1821
Cdd:cd05970   258 YDYDKFDPKALLEKLSKYGVTTFCAPPTIYRFLIREDLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIKLMEGFGQTE 337

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1822 AAIDVTFWACQPDDHRSfvpIGRPIANTQLYILDTLGQPVPLGVAGELHIG-----GVGVARGYLNRPDLTAERFipdpf 1896
Cdd:cd05970   338 TTLTIATFPWMEPKPGS---MGKPAPGYEIDLIDREGRSCEAGEEGEIVIRtskgkPVGLFGGYYKDAEKTAEVW----- 409

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1897 inQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQP 1976
Cdd:cd05970   410 --HDG--YYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLAK 485

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967 1977 GVTPDPAdLRQQLGQHL----AEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05970   486 GYEPSEE-LKKELQDHVkkvtAPYKYPRIVEFVDELPKTISGKIRRVEI 533
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
 1555-2021 2.27e-23

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 107.53  E-value: 2.27e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1555 TYDALNRRANQLAHHLIDLGVKPDDRIAIC---VERSLDMVIGLLAIlkaGAAYVPLDPGYPAERLAYMLDDASPVALLT 1631
Cdd:PRK06018   41 TYAQIHDRALKVSQALDRDGIKLGDRVATIawnTWRHLEAWYGIMGI---GAICHTVNPRLFPEQIAWIINHAEDRVVIT 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1632 QANQRALLTGDVPRI-----LLDTADFSHLSEdnPHVPGL-------------------DAHHLAYVIYTSGSTGKPKGV 1687
Cdd:PRK06018  118 DLTFVPILEKIADKLpsverYVVLTDAAHMPQ--TTLKNAvayeewiaeadgdfawktfDENTAAGMCYTSGTTGDPKGV 195

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1688 MNSHRA--LCNRLVWMQNTYRLTPDDRVLQKTPFsFDVSVW--EFFWPLLyGARLVMarPDGHKDAAYLAQLIERTGITT 1763
Cdd:PRK06018  196 LYSHRSnvLHALMANNGDALGTSAADTMLPVVPL-FHANSWgiAFSAPSM-GTKLVM--PGAKLDGASVYELLDTEKVTF 271

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1764 LHFVPSMLQQFVQWADADCA-CDSLRRVICSGEALPAELQqRFFARFNAQLHNLYGPTEAAIDVTFWACQP------DDH 1836
Cdd:PRK06018  272 TAGVPTVWLMLLQYMEKEGLkLPHLKMVVCGGSAMPRSMI-KAFEDMGVEVRHAWGMTEMSPLGTLAALKPpfsklpGDA 350

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1837 RSFVPI--GRPIANTQLYILDTLGQPVPL-GVA-GELHIGGVGVARGYLNRPD--LTAERFipdpfinqpgarlYKTGDL 1910
Cdd:PRK06018  351 RLDVLQkqGYPPFGVEMKITDDAGKELPWdGKTfGRLKVRGPAVAAAYYRVDGeiLDDDGF-------------FDTGDV 417

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1911 ARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLG 1990
Cdd:PRK06018  418 ATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLIVQLKPGETATREEILKYMD 497

                         490       500       510
                  ....*....|....*....|....*....|.
gi 641744967 1991 QHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK06018  498 GKIAKWWMPDDVAFVDAIPHTATGKILKTAL 528
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
 1554-2021 2.55e-23

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 107.54  E-value: 2.55e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDL-GVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQ 1632
Cdd:PRK05677   50 LTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKALVCL 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1633 ANQRALLTGDVPR------ILLDTADF-------------SHLSEDNP--HVPG--------------------LDAHHL 1671
Cdd:PRK05677  130 ANMAHLAEKVLPKtgvkhvIVTEVADMlpplkrllinavvKHVKKMVPayHLPQavkfndalakgagqpvteanPQADDV 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1672 AYVIYTSGSTGKPKGVMNSHRAL-CNRLVWMQNTYRLTPDDRVLQKTP--------FSFDVSVWeffwpLLYGARLVMar 1742
Cdd:PRK05677  210 AVLQYTGGTTGVAKGAMLTHRNLvANMLQCRALMGSNLNEGCEILIAPlplyhiyaFTFHCMAM-----MLIGNHNIL-- 282

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1743 pdghkdaaylaqliertgITTLHFVPSMLQQFVQWA-----------DADCACDSLRRV--------ICSGEALPAELQQ 1803
Cdd:PRK05677  283 ------------------ISNPRDLPAMVKELGKWKfsgfvglntlfVALCNNEAFRKLdfsalkltLSGGMALQLATAE 344

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1804 RFFARFNAQLHNLYGPTEAAIDVTFwacQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNR 1883
Cdd:PRK05677  345 RWKEVTGCAICEGYGMTETSPVVSV---NPSQAIQVGTIGIPVPSTLCKVIDDDGNELPLGEVGELCVKGPQVMKGYWQR 421

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1884 PDLTAERFIPDPFInqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSP 1963
Cdd:PRK05677  422 PEATDEILDSDGWL--------KTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEK 493

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 1964 GDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK05677  494 SGEAIKVFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRREL 551
PLN02574 PLN02574
4-coumarate--CoA ligase-like
 1554-2021 3.16e-23

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 107.24  E-value: 3.16e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLID-LGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDP-GYPAERLAYMLDDASPVALLT 1631
Cdd:PLN02574   67 ISYSELQPLVKSMAAGLYHvMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPsSSLGEIKKRVVDCSVGLAFTS 146

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1632 QANQRALLTGDVPRILL-DTADFSHLSEDNPHV-------------PGLDAHHLAYVIYTSGSTGKPKGVMNSHR---AL 1694
Cdd:PLN02574  147 PENVEKLSPLGVPVIGVpENYDFDSKRIEFPKFyelikedfdfvpkPVIKQDDVAAIMYSSGTTGASKGVVLTHRnliAM 226

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1695 CNRLVWMQNT-YRLTPDDRV-LQKTPFSFDVSVWEFFWPLL-YGARLVMARpdgHKDAAYLAQLIERTGITTLHFVPSML 1771
Cdd:PLN02574  227 VELFVRFEASqYEYPGSDNVyLAALPMFHIYGLSLFVVGLLsLGSTIVVMR---RFDASDMVKVIDRFKVTHFPVVPPIL 303

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1772 QQFVQWADADCA--CDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIdVTFWACQPDDHRSFVPIGRPIAN 1848
Cdd:PLN02574  304 MALTKKAKGVCGevLKSLKQVSCGAAPLSGKFIQDFVQTLpHVDFIQGYGMTESTA-VGTRGFNTEKLSKYSSVGLLAPN 382

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1849 TQLYILD-TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFInqpgarlyKTGDLARWLPDGSLEYLGRNDF 1927
Cdd:PLN02574  383 MQAKVVDwSTGCLLPPGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGWL--------RTGDIAYFDEDGYLYIVDRLKE 454

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1928 QVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDA 2007
Cdd:PLN02574  455 IIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQEAVINYVAKQVAPYKKVRKVVFVQS 534

                         490
                  ....*....|....
gi 641744967 2008 FPLTPNGKLDRKAL 2021
Cdd:PLN02574  535 IPKSPAGKILRREL 548
PRK05850 PRK05850
acyl-CoA synthetase; Validated
 2606-3000 4.42e-23

acyl-CoA synthetase; Validated


Pssm-ID: 235624 [Multi-domain]  Cd Length: 578  Bit Score: 106.95  E-value: 4.42e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2606 ALIHELFEAQvactPDAIAVVF--------GEA-SLSYDELNRRANRLAHHLISFGVrPDERVAICVERGLDMVVGLLGI 2676
Cdd:PRK05850    5 SLLRERASLQ----PDDAAFTFidyeqdpaGVAeTLTWSQLYRRTLNVAEELRRHGS-TGDRAVILAPQGLEYIVAFLGA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2677 LKAGGAYVPLD-PTYPV--ERLRYMLDDAKPVALISQSAhlgIMNGSLPVILLDDGETRPFDNEPDTP-LDA-RKQGLTP 2751
Cdd:PRK05850   80 LQAGLIAVPLSvPQGGAhdERVSAVLRDTSPSVVLTTSA---VVDDVTEYVAPQPGQSAPPVIEVDLLdLDSpRGSDARP 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2752 RHL---AYVIYTSGSTGKPKGVMVEHAN-MVNFLCSMR----KEPGIAQEDVLLgVTSLSF--DIS-ILEIFLPLLNGAR 2820
Cdd:PRK05850  157 RDLpstAYLQYTSGSTRTPAGVMVSHRNvIANFEQLMSdyfgDTGGVPPPDTTV-VSWLPFyhDMGlVLGVCAPILGGCP 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2821 LILATqaqaadaqqlamlierhAVSFMQaTPSTW-RMLVE-LRDFALPPGFK-ALCGGEALPENLATALLQKVTTL---- 2893
Cdd:PRK05850  236 AVLTS-----------------PVAFLQ-RPARWmQLLASnPHAFSAAPNFAfELAVRKTSDDDMAGLDLGGVLGIisgs 297

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2894 -----------------WNL--------YGPTETTIW-STLNGLTTP-----------------------TPYIGHPIAN 2924
Cdd:PRK05850  298 ervhpatlkrfadrfapFNLretairpsYGLAEATVYvATREPGQPPesvrfdyeklsaghakrcetgggTPLVSYGSPR 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2925 TQIY-ILDAQGRV-VPLGVAGEIHIAGAGVVRGYLGRPDLTAERF---ITDPFSGAPEARMYKTGDLGrWLPDGTLEYLG 2999
Cdd:PRK05850  378 SPTVrIVDPDTCIeCPAGTVGEIWVHGDNVAAGYWQKPEETERTFgatLVDPSPGTPEGPWLRTGDLG-FISEGELFIVG 456


                  .
gi 641744967 3000 R 3000
Cdd:PRK05850  457 R 457
AcpA COG3433
Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites ...
 780-1056 4.45e-23

Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442659 [Multi-domain]  Cd Length: 295  Bit Score: 102.52  E-value: 4.45e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  780 PLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFSADPaariYKTGDLARWLPDG 859
Cdd:COG3433    16 PPPVIPPAIVQARALLLIVDLQGYFGGFGGEGGLLGAGLLLRIRLLAAAARAPFIPVPYPAQP----GRQADDLRLLLRR 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  860 NIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCA--RPDAELHPAALRQQLAASLAD 937
Cdd:COG3433    92 GLGPGGGLERLVQQVVIRAERGEEEELLLVLRAAAVVRVAVLAALRGAGVGLLLIVGavAALDGLAAAAALAALDKVPPD 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  938 YMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAFATRDYEAP---QGGIETALAALWQELLGL--DRVGRHDQFFALGGH 1012
Cdd:COG3433   172 VVAASAVVALDALLLLALKVVARAAPALAAAEALLAAASPAPaleTALTEEELRADVAELLGVdpEEIDPDDNLFDLGLD 251

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....
gi 641744967 1013 SLLAVQLLNRMNKAGMDVALATLFAHPTLCDLAAAVTATAHDAP 1056
Cdd:COG3433   252 SIRLMQLVERWRKAGLDVSFADLAEHPTLAAWWALLAAAQAAAA 295
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
 1532-2021 5.46e-23

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 106.64  E-value: 5.46e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1532 QLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:PRK07059   27 DLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPL 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 YPAERLAYMLDDASPVALLTQAN-----QRALLTGDVPRILL----DTADF----------------------------S 1654
Cdd:PRK07059  107 YTPRELEHQLKDSGAEAIVVLENfattvQQVLAKTAVKHVVVasmgDLLGFkghivnfvvrrvkkmvpawslpghvrfnD 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1655 HLSED-----NPhvPGLDAHHLAYVIYTSGSTGKPKGVMNSHR-ALCNRL---VWMQNTYRLTPDDRVLQ---KTPFS-- 1720
Cdd:PRK07059  187 ALAEGarqtfKP--VKLGPDDVAFLQYTGGTTGVSKGATLLHRnIVANVLqmeAWLQPAFEKKPRPDQLNfvcALPLYhi 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1721 FDVSVWEFFWPLLYGARLVMARPdghKDAAYLAQLIERTGITTLHFVPSMLQQFVQWAD-ADCACDSLRRVICSGEALPA 1799
Cdd:PRK07059  265 FALTVCGLLGMRTGGRNILIPNP---RDIPGFIKELKKYQVHIFPAVNTLYNALLNNPDfDKLDFSKLIVANGGGMAVQR 341

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1800 ELQQRFFARFNAQLHNLYGPTEAAIDVTfwaCQPDDHRSFV-PIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVAR 1878
Cdd:PRK07059  342 PVAERWLEMTGCPITEGYGLSETSPVAT---CNPVDATEFSgTIGLPLPSTEVSIRDDDGNDLPLGEPGEICIRGPQVMA 418

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1879 GYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVA 1958
Cdd:PRK07059  419 GYWNRPDETAKVMTADGF--------FRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLEVAAVG 490

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 1959 REDSPGDTRLVAYLcpqpgVTPDPADLRQQLGQHLAE----YMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK07059  491 VPDEHSGEAVKLFV-----VKKDPALTEEDVKAFCKErltnYKRPKFVEFRTELPKTNVGKILRREL 552
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
 2757-3094 5.59e-23

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 103.12  E-value: 5.59e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2757 VIYTSGSTGKPKGVMVEHANMVnfLCSMRKEP--GIAQEDVLLGVTSLsFDISILEIFLPLLN--GARLILATQAQAADA 2832
Cdd:cd17637     5 IIHTAAVAGRPRGAVLSHGNLI--AANLQLIHamGLTEADVYLNMLPL-FHIAGLNLALATFHagGANVVMEKFDPAEAL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2833 QqlamLIERHAVSFMQATPSTWRMLVELRDfALPPGFKAL--CGGEALPENLAtaLLQKVT--TLWNLYGPTETTiwstl 2908
Cdd:cd17637    82 E----LIEEEKVTLMGSFPPILSNLLDAAE-KSGVDLSSLrhVLGLDAPETIQ--RFEETTgaTFWSLYGQTETS----- 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 nGLTTPTPY------IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDpfsgapearMYK 2982
Cdd:cd17637   150 -GLVTLSPYrerpgsAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYTFRNG---------WHH 219

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2983 TGDLGRWLPDGTLEYLGRNDFQ--VKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPAD 3060
Cdd:cd17637   220 TGDLGRFDEDGYLWYAGRKPEKelIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPGATLTADE 299

                         330       340       350
                  ....*....|....*....|....*....|....
gi 641744967 3061 LRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDR 3094
Cdd:cd17637   300 LIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
 619-960 6.02e-23

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 103.12  E-value: 6.02e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  619 VIYTSGSTGRPKGVMVAHRNVI------NLATGLH------TLLALDHPSriALNASI-VFDASVKNwiqllsghtlVLV 685
Cdd:cd17637     5 IIHTAAVAGRPRGAVLSHGNLIaanlqlIHAMGLTeadvylNMLPLFHIA--GLNLALaTFHAGGAN----------VVM 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  686 PdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAisPAVWSRLQSLSDTRFINVYGPTE 765
Cdd:cd17637    73 E---KFDPAEALELIEEEKVTLMGSFPPILSNLLDAAEKSGVDLSSLRHVLGLDA--PETIQRFEETTGATFWSLYGQTE 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  766 ctvdaTACVVD----RTQPlPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFipdpfsad 841
Cdd:cd17637   148 -----TSGLVTlspyRERP-GSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYTF-------- 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  842 paaR--IYKTGDLARWLPDGNIDYLGRNDFQ--IKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDsPGDTRLVAYLCA- 916
Cdd:cd17637   214 ---RngWHHTGDLGRFDEDGYLWYAGRKPEKelIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPD-PKWGEGIKAVCVl 289

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....
gi 641744967  917 RPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDR 960
Cdd:cd17637   290 KPGATLTADELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
 2625-3097 6.27e-23

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 106.22  E-value: 6.27e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2625 VVFGEA----SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLD 2700
Cdd:PLN02330   45 VAFVEAvtgkAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTALESEIKKQAE 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2701 DAKPVALISQSAHLGIMNG-SLPVILLDDGETRPFDNEPDTPLDARKQGLTPRH-------LAYVIYTSGSTGKPKGVMV 2772
Cdd:PLN02330  125 AAGAKLIVTNDTNYGKVKGlGLPVIVLGEEKIEGAVNWKELLEAADRAGDTSDNeeilqtdLCALPFSSGTTGISKGVML 204

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2773 EHANMVNFLCS--MRKEPGIAQEDVLLGVTSLSFDISILEI-FLPLLNGARLILATQAQAADAQQLAMlieRHAVSFMQA 2849
Cdd:PLN02330  205 THRNLVANLCSslFSVGPEMIGQVVTLGLIPFFHIYGITGIcCATLRNKGKVVVMSRFELRTFLNALI---TQEVSFAPI 281

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2850 TPSTWRMLVE---LRDFALPP-GFKALCGGEA-LPENLATALLQKV--TTLWNLYGPTETTIWStlngLTTPTPYIGHPI 2922
Cdd:PLN02330  282 VPPIILNLVKnpiVEEFDLSKlKLQAIMTAAApLAPELLTAFEAKFpgVQVQEAYGLTEHSCIT----LTHGDPEKGHGI 357

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2923 A----------NTQIYILDAQ-GRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLP 2991
Cdd:PLN02330  358 AkknsvgfilpNLEVKFIDPDtGRSLPKNTPGELCVRSQCVMQGYYNNKEETDRTIDEDGW--------LHTGDIGYIDD 429

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2992 DGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAE 3071
Cdd:PLN02330  430 DGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACVVINPKAKESEEDILNFVAANVAH 509

                         490       500
                  ....*....|....*....|....*.
gi 641744967 3072 YMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PLN02330  510 YKKVRVVQFVDSIPKSLSGKIMRRLL 535
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
 2625-3094 1.03e-22

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 104.45  E-value: 1.03e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2625 VVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKP 2704
Cdd:cd05914     1 LYYGGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2705 VALisqsahlgimngslpvillddgetrpFDNEPDtpldarkqgltprHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSM 2784
Cdd:cd05914    81 KAI--------------------------FVSDED-------------DVALINYTSGTTGNSKGVMLTYRNIVSNVDGV 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2785 RKEPGIAQEDVLLGVTSLSFDISILEIFL-PLLNGA-----------RLILATQAQAADAQQLAMLIERHAVSFMQATPS 2852
Cdd:cd05914   122 KEVVLLGKGDKILSILPLHHIYPLTFTLLlPLLNGAhvvfldkipsaKIIALAFAQVTPTLGVPVPLVIEKIFKMDIIPK 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2853 T------WRMLVELRDF------------ALPPGFKALC-GGEALPENLATALLQKVTTLWNLYGPTET------TIWST 2907
Cdd:cd05914   202 LtlkkfkFKLAKKINNRkirklafkkvheAFGGNIKEFViGGAKINPDVEEFLRTIGFPYTIGYGMTETapiisySPPNR 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2908 LNGLTTptpyiGHPIANTQIYILDAQgrvvPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLG 2987
Cdd:cd05914   282 IRLGSA-----GKVIDGVEVRIDSPD----PATGEGEIIVRGPNVMKGYYKNPEATAEAFDKDGW--------FHTGDLG 344

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2988 RWLPDGTLEYLGR-NDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREdspgdKRLVAYLLAQPDTVLEPADLRQRLS 3066
Cdd:cd05914   345 KIDAEGYLYIRGRkKEMIVLSSGKNIYPEEIEAKINNMPFVLESLVVVQE-----KKLVALAYIDPDFLDVKALKQRNII 419

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 641744967 3067 EGVAEYMIPSAFVTLDA-------------FPLTPNGKLDR 3094
Cdd:cd05914   420 DAIKWEVRDKVNQKVPNykkiskvkivkeeFEKTPKGKIKR 460
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
 1552-1977 1.05e-22

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 104.99  E-value: 1.05e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1552 QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGA----AYVPLdpGYPAerLAYMLDDASPV 1627
Cdd:cd17639     4 KYMSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIpivtVYATL--GEDA--LIHSLNETECS 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1628 ALLTQANqralltgdvprilldtadfshlSEDnphvpgldahhLAYVIYTSGSTGKPKGVMNSHR-------ALCNRLVw 1700
Cdd:cd17639    80 AIFTDGK----------------------PDD-----------LACIMYTSGSTGNPKGVMLTHGnlvagiaGLGDRVP- 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1701 mqntYRLTPDDRVLQKTP----FSFDVSVWEFFWpllyGARL-----------VMARPDGhkDA---------------- 1749
Cdd:cd17639   126 ----ELLGPDDRYLAYLPlahiFELAAENVCLYR----GGTIgygsprtltdkSKRGCKG--DLtefkptlmvgvpaiwd 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1750 ----AYLAQLIERTGI--TTLHFVPSMLQQFVQWADADCACDSL-----R-------RVICSGEALPAELQQRFFARFNA 1811
Cdd:cd17639   196 tirkGVLAKLNPMGGLkrTLFWTAYQSKLKALKEGPGTPLLDELvfkkvRaalggrlRYMLSGGAPLSADTQEFLNIVLC 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1812 QLHNLYGPTE--AAIDVTFWACQPDDHrsfvpIGRPIANTQLYILDT--LG----QPVPlgvAGELHIGGVGVARGYLNR 1883
Cdd:cd17639   276 PVIQGYGLTEtcAGGTVQDPGDLETGR-----VGPPLPCCEIKLVDWeeGGystdKPPP---RGEILIRGPNVFKGYYKN 347

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1884 PDLTAERFIPDpfinqpgaRLYKTGDLARWLPDGSLEYLGRNDFQVKLR-GFRIELGEIEARLMQCPGVQEAVVVAredS 1962
Cdd:cd17639   348 PEKTKEAFDGD--------GWFHTGDIGEFHPDGTLKIIDRKKDLVKLQnGEYIALEKLESIYRSNPLVNNICVYA---D 416

                         490
                  ....*....|....*
gi 641744967 1963 PGDTRLVAYLCPQPG 1977
Cdd:cd17639   417 PDKSYPVAIVVPNEK 431
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
 615-960 1.42e-22

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 101.71  E-value: 1.42e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  615 HLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQLL-SGHTLVLVPdalRADA 693
Cdd:cd17633     1 NPFYIGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSHSLFLYGAISALyLGGTFIGQR---KFNP 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  694 HQLWRYFARHAVDLFDCTPVQLQWLLDAGlgsDPAYQPAQVLIGGEAISPAVWSRLQSLS-DTRFINVYGPTECTVdATA 772
Cdd:cd17633    78 KSWIRKINQYNATVIYLVPTMLQALARTL---EPESKIKSIFSSGQKLFESTKKKLKNIFpKANLIEFYGTSELSF-ITY 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  773 CVVDRTQPLPTIGKPLANTRLYILDAQDqpvpiGVTGELHIGGAGVARGYLHRPDLTAERFipdpfsadpaariYKTGDL 852
Cdd:cd17633   154 NFNQESRPPNSVGRPFPNVEIEIRNADG-----GEIGKIFVKSEMVFSGYVRGGFSNPDGW-------------MSVGDI 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  853 ARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARpdaELHPAALRQQLA 932
Cdd:cd17633   216 GYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALYSGD---KLTYKQLKRFLK 292

                         330       340
                  ....*....|....*....|....*...
gi 641744967  933 ASLADYMIPSAFVTLDALPLTPNGKLDR 960
Cdd:cd17633   293 QKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
FACL_like_5 cd05924
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 2756-3093 1.52e-22

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341248 [Multi-domain]  Cd Length: 364  Bit Score: 102.46  E-value: 1.52e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2756 YVIYTSGSTGKPKGVMVEH----------ANMVNFLCSMRKEPGIAQED----VLLGVTSLSFDISILEIFLPLLNGARL 2821
Cdd:cd05924     7 YILYTGGTTGMPKGVMWRQedifrmlmggADFGTGEFTPSEDAHKAAAAaagtVMFPAPPLMHGTGSWTAFGGLLGGQTV 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2822 ILATQAQAADAQQLamLIERHAVSFMQ------ATPstwrMLVELRD---FALPPGFKALCGGEALPENLATALLQKV-- 2890
Cdd:cd05924    87 VLPDDRFDPEEVWR--TIEKHKVTSMTivgdamARP----LIDALRDagpYDLSSLFAISSGGALLSPEVKQGLLELVpn 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2891 TTLWNLYGPTETTiwSTLNGLTTPTPYIGHP--IANTQIYILDAQGRVVPLGVAGEIHIAGAGVV-RGYLGRPDLTAERF 2967
Cdd:cd05924   161 ITLVDAFGSSETG--FTGSGHSAGSGPETGPftRANPDTVVLDDDGRVVPPGSGGVGWIARRGHIpLGYYGDEAKTAETF 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2968 ITdpfsgAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAY 3047
Cdd:cd05924   239 PE-----VDGVRYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQEVVAV 313

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 3048 LLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLD 3093
Cdd:cd05924   314 VQLREGAGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGKAD 359
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
 1537-2016 1.58e-22

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 104.71  E-value: 1.58e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1537 QAARTPDTTAVLFED--QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPA 1614
Cdd:PRK13390    6 HAQIAPDRPAVIVAEtgEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTA 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1615 ERLAYMLDDASPVALLTQANQRALLT---GDVP-RILLDT-----ADF-SHLSEDNPhvPGLDAHHLAYVIYTSGSTGKP 1684
Cdd:PRK13390   86 PEADYIVGDSGARVLVASAALDGLAAkvgADLPlRLSFGGeidgfGSFeAALAGAGP--RLTEQPCGAVMLYSSGTTGFP 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1685 KGVMNShraLCNRLVwmqntyrLTPDDRVLQKTPFSFDVSVWEFFW---------PLLY-------GARLVMARpdgHKD 1748
Cdd:PRK13390  164 KGIQPD---LPGRDV-------DAPGDPIVAIARAFYDISESDIYYssapiyhaaPLRWcsmvhalGGTVVLAK---RFD 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1749 AAYLAQLIERTGITTLHFVPSMLQQFVQW-ADADCACD--SLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEA--- 1822
Cdd:PRK13390  231 AQATLGHVERYRITVTQMVPTMFVRLLKLdADVRTRYDvsSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEYYSSTEAhgm 310

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1823 -AIDVTFWACQPDDhrsfvpIGRPIANTqLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAErfipdpfINQPG 1901
Cdd:PRK13390  311 tFIDSPDWLAHPGS------VGRSVLGD-LHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAA-------AQHPA 376

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1902 ARLYKT-GDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTP 1980
Cdd:PRK13390  377 HPFWTTvGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRG 456

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967 1981 DPADLRQQLG---QHLAEYMVPGAFVTLDAFPLTPNGKL 2016
Cdd:PRK13390  457 SDELARELIDytrSRIAHYKAPRSVEFVDELPRTPTGKL 495
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
 619-958 2.09e-22

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 101.42  E-value: 2.09e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  619 VIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVK-NWIQ-LLSGHTlvLVPDALrADAHQL 696
Cdd:cd17638     5 IMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYLIINPFFHTFGYKaGIVAcLLTGAT--VVPVAV-FDVDAI 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  697 WRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVW-SRLQS-LSDTRFINVYGPTECTVdATACV 774
Cdd:cd17638    82 LEAIERERITVLPGPPTLFQSLLDHPGRKKFDLSSLRAAVTGAATVPVELvRRMRSeLGFETVLTAYGLTEAGV-ATMCR 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  775 V--DRTQPLPTIGKPLANTRLYILDAqdqpvpigvtGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDL 852
Cdd:cd17638   161 PgdDAETVATTCGRACPGFEVRIADD----------GEVLVRGYNVMQGYLDDPEATAEAIDADGW--------LHTGDV 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  853 ARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLA 932
Cdd:cd17638   223 GELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGVTLTEEDVIAWCR 302

                         330       340
                  ....*....|....*....|....*.
gi 641744967  933 ASLADYMIPSAFVTLDALPLTPNGKL 958
Cdd:cd17638   303 ERLANYKVPRFVRFLDELPRNASGKV 328
PLN02574 PLN02574
4-coumarate--CoA ligase-like
 499-963 2.32e-22

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 104.54  E-value: 2.32e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQ 577
Cdd:PLN02574   67 ISYSELQPLVKSMAAGLYhVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTS 146

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  578 SAQVAQLNST-LPTVLL------DTPAAAACP-------DTNPVVQGLHAAH-LAYVIYTSGSTGRPKGVMVAHRNVINL 642
Cdd:PLN02574  147 PENVEKLSPLgVPVIGVpenydfDSKRIEFPKfyelikeDFDFVPKPVIKQDdVAAIMYSSGTTGASKGVVLTHRNLIAM 226

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  643 atgLHTLLALDHPSRIALNASIVFDASVKNW---------IQLLS-GHTLVLVPdalRADAHQLWRYFARHAVDLFDCTP 712
Cdd:PLN02574  227 ---VELFVRFEASQYEYPGSDNVYLAALPMFhiyglslfvVGLLSlGSTIVVMR---RFDASDMVKVIDRFKVTHFPVVP 300

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  713 VQLQWLLDA--GLGSDPAYQPAQVLIGGEAIS-PAVWSRLQSLSDTRFINVYGPTECTVDATACV-VDRTQPLPTIGKPL 788
Cdd:PLN02574  301 PILMALTKKakGVCGEVLKSLKQVSCGAAPLSgKFIQDFVQTLPHVDFIQGYGMTESTAVGTRGFnTEKLSKYSSVGLLA 380

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  789 ANTRLYILDAQDQP-VPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRN 867
Cdd:PLN02574  381 PNMQAKVVDWSTGClLPPGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGW--------LRTGDIAYFDEDGYLYIVDRL 452

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  868 DFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTL 947
Cdd:PLN02574  453 KEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQEAVINYVAKQVAPYKKVRKVVFV 532

                         490
                  ....*....|....*.
gi 641744967  948 DALPLTPNGKLDRKAL 963
Cdd:PLN02574  533 QSIPKSPAGKILRREL 548
FATP_FACS cd05940
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ...
 496-941 2.81e-22

Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341263 [Multi-domain]  Cd Length: 449  Bit Score: 103.20  E-value: 2.81e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  496 DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALL 575
Cdd:cd05940     1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  576 tqsaqvaqlnstlptvlldtpaaaacpdtnpvvqglhaAHLAYVIYTSGSTGRPKGVMVAHRNVINLATG---------- 645
Cdd:cd05940    81 --------------------------------------VDAALYIYTSGTTGLPKAAIISHRRAWRGGAFfagsggalps 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  646 --LHTLLALDHPsrialNASIVFDASVknwiqLLSGHTLVLvpdALRADAHQLWRYFARHavdlfDCTPVQ-----LQWL 718
Cdd:cd05940   123 dvLYTCLPLYHS-----TALIVGWSAC-----LASGATLVI---RKKFSASNFWDDIRKY-----QATIFQyigelCRYL 184

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  719 LDaglgsdpayQPAQV--------LIGGEAISPAVWSRLQslsdTRFiNV------YGPTECT-----VDATACVVDRTQ 779
Cdd:cd05940   185 LN---------QPPKPterkhkvrMIFGNGLRPDIWEEFK----ERF-GVpriaefYAATEGNsgfinFFGKPGAIGRNP 250

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  780 PLPTIGKPLANTRlYILDAQD---------QPVPIGVTGEL--HIGGAGVARGYLhRPDLTAERFIPDPFSADPAAriYK 848
Cdd:cd05940   251 SLLRKVAPLALVK-YDLESGEpirdaegrcIKVPRGEPGLLisRINPLEPFDGYT-DPAATEKKILRDVFKKGDAW--FN 326

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  849 TGDLARWLPDGNIDYLGR--NDFQIKvrGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRL-VAYLCARPDAELHPA 925
Cdd:cd05940   327 TGDLMRLDGEGFWYFVDRlgDTFRWK--GENVSTTEVAAVLGAFPGVEEANVYGVQVPGTDGRAgMAAIVLQPNEEFDLS 404

                         490
                  ....*....|....*.
gi 641744967  926 ALRQQLAASLADYMIP 941
Cdd:cd05940   405 ALAAHLEKNLPGYARP 420
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
 2624-3100 3.91e-22

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 103.24  E-value: 3.91e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2624 AVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAK 2703
Cdd:PRK12406    4 TIISGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSG 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2704 PVALIsqsAHLGIMNG-------SLPVILL--------------DDGETRPFDNEPDTPLDARK--QGLTPRHLAYVIYT 2760
Cdd:PRK12406   84 ARVLI---AHADLLHGlasalpaGVTVLSVptppeiaaayrispALLTPPAGAIDWEGWLAQQEpyDGPPVPQPQSMIYT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2761 SGSTGKPKGVmvehanmvnflcsmRKEPG-----IAQEDVLLGVTSLSFDISILeIFLPLLN-------------GARLI 2822
Cdd:PRK12406  161 SGTTGHPKGV--------------RRAAPtpeqaAAAEQMRALIYGLKPGIRAL-LTGPLYHsapnayglragrlGGVLV 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2823 LATQAQAADAQQlamLIERHAVSFMQATPStwrMLVELRDfaLPPGFKA----------LCGGEALPENLATALLQkvtt 2892
Cdd:PRK12406  226 LQPRFDPEELLQ---LIERHRITHMHMVPT---MFIRLLK--LPEEVRAkydvsslrhvIHAAAPCPADVKRAMIE---- 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2893 lW------NLYGPTET---TIWSTLNGLTTPTPyIGHPIANTQIYILDAQGRVVPLGVAGEI--HIAGAGVVRgYLGRPD 2961
Cdd:PRK12406  294 -WwgpviyEYYGSTESgavTFATSEDALSHPGT-VGKAAPGAELRFVDEDGRPLPQGEIGEIysRIAGNPDFT-YHNKPE 370

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2962 LTAE----RFITdpfsgapearmykTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIARED 3037
Cdd:PRK12406  371 KRAEidrgGFIT-------------SGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPD 437

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967 3038 SPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAP 3100
Cdd:PRK12406  438 AEFGEALMAVVEPQPGATLDEADIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDP 500
PRK13382 PRK13382
bile acid CoA ligase;
2611-3100 4.57e-22

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 103.30  E-value: 4.57e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2611 LFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTY 2690
Cdd:PRK13382   48 GFAIAAQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSF 127

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2691 PVERLRYMLDDAKPVALISQSAHLGimngSLPVILLDDGETRPFDNEPDTPLDARKQGL-----------TPRHLAYVIY 2759
Cdd:PRK13382  128 AGPALAEVVTREGVDTVIYDEEFSA----TVDRALADCPQATRIVAWTDEDHDLTVEVLiaahagqrpepTGRKGRVILL 203

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2760 TSGSTGKPKGVMVEHANMVNFLCS-MRKEPGIAQEDVLLgVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQlamL 2838
Cdd:PRK13382  204 TSGTTGTPKGARRSGPGGIGTLKAiLDRTPWRAEEPTVI-VAPMFHAWGFSQLVLAASLACTIVTRRRFDPEATLD---L 279

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2839 IERHAVSFMQATPSTWRMLVELRDFALPP----GFK-ALCGGEALPENLATALLQKV-TTLWNLYGPTETTIWSTLN--G 2910
Cdd:PRK13382  280 IDRHRATGLAVVPVMFDRIMDLPAEVRNRysgrSLRfAAASGSRMRPDVVIAFMDQFgDVIYNNYNATEAGMIATATpaD 359

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2911 LTTPTPYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYL-GRPDLTAERFITdpfsgapearmykTGDLGRW 2989
Cdd:PRK13382  360 LRAAPDTAGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGYTsGSTKDFHDGFMA-------------SGDVGYL 426

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2990 LPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGV 3069
Cdd:PRK13382  427 DENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGASATPETLKQHVRDNL 506

                         490       500       510
                  ....*....|....*....|....*....|.
gi 641744967 3070 AEYMIPSAFVTLDAFPLTPNGKLDRKALPAP 3100
Cdd:PRK13382  507 ANYKVPRDIVVLDELPRGATGKILRRELQAR 537
SgcC5_NRPS-like cd19539
SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- ...
 2163-2509 4.65e-22

SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- bond forming activity and similar C-domains of modular NRPSs; SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. This subfamily also includes similar C-domains of modular NRPSs such as Penicillium chrysogenum N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase PCBAB. Condensation (C) domains of NRPSs normally catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380462 [Multi-domain]  Cd Length: 427  Bit Score: 102.07  E-value: 4.65e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2163 PLAPLQEGILFHHllqeQGDTYLLRSMVAFTHRER----LDAFLSALQQVIDRHDILRTAVCWQDLSQPVQVVWRQAILP 2238
Cdd:cd19539     3 PLSFAQERLWFID----QGEDGGPAYNIPGAWRLTgpldVEALREALRDVVARHEALRTLLVRDDGGVPRQEILPPGPAP 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2239 INHF-EPTSPEDVLAQLQAH-TEPRTRRIDLSQAPLFRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQ 2316
Cdd:cd19539    79 LEVRdLSDPDSDRERRLEELlRERESRGFDLDEEPPIRAVLGRFD-PDDHVLVLVAHHTAFDAWSLDVFARDLAALYAAR 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2317 ADALPTPLP-----YRNFIA---QTLSVPNSAHE-AYFRDKLADVDEPTAPFGLLNVQGSGGDIHEARLVLDATLASAIR 2387
Cdd:cd19539   158 RKGPAAPLPelrqqYKEYAAwqrEALAAPRAAELlDFWRRRLRGAEPTALPTDRPRPAGFPYPGADLRFELDAELVAALR 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2388 QQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISLADRGA-AEVVERTSHD 2466
Cdd:cd19539   238 ELAKRARSSLFMVLLAAYCVLLRRYTGQTDIVVGTPVAGR--NHPRFESTVGFFVNLLPLRVDVSDCATfRDLIARVRKA 315

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*...
gi 641744967 2467 LMTLLEHEQAPlaLAQRCSGVAPPM-----PLFSTLLNYRHTQASSTD 2509
Cdd:cd19539   316 LVDAQRHQELP--FQQLVAELPVDRdagrhPLVQIVFQVTNAPAGELE 361
PRK07529 PRK07529
AMP-binding domain protein; Validated
 2609-3134 4.71e-22

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 104.27  E-value: 4.71e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2609 HELFEAQVACTPDAIAVVF---GEA-----SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLG----- 2675
Cdd:PRK07529   28 YELLSRAAARHPDAPALSFlldADPldrpeTWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGgeaag 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2676 ----------------ILKAGGA--YVPLDPTyP-------VERLRYMLDDAKPVALISQSAHLGIMNGSLPVILLDDGE 2730
Cdd:PRK07529  108 ianpinpllepeqiaeLLRAAGAkvLVTLGPF-PgtdiwqkVAEVLAALPELRTVVEVDLARYLPGPKRLAVPLIRRKAH 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2731 TRPFD------NEPDTPLDARKQgLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLsF 2804
Cdd:PRK07529  187 ARILDfdaelaRQPGDRLFSGRP-IGPDDVAAYFHTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPGDTVFCGLPL-F 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2805 DI--SILEIFLPLLNGARLILATQA---QAADAQQLAMLIERHAVSFMQATPSTWRMLVelrdfALPPGFK-------AL 2872
Cdd:PRK07529  265 HVnaLLVTGLAPLARGAHVVLATPQgyrGPGVIANFWKIVERYRINFLSGVPTVYAALL-----QVPVDGHdisslryAL 339

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2873 CGGEALPENLATAlLQKVT--TLWNLYGPTETTIWSTLNGLTTP--TPYIGHPIANTQ--IYILDAQGRVV---PLGVAG 2943
Cdd:PRK07529  340 CGAAPLPVEVFRR-FEAATgvRIVEGYGLTEATCVSSVNPPDGErrIGSVGLRLPYQRvrVVILDDAGRYLrdcAVDEVG 418

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2944 EIHIAGAGVVRGYLgRPDLTAERFItdpfsgapEARMYKTGDLGRWLPDGTLEYLGRndfqVK---VR-GFRIELGEIET 3019
Cdd:PRK07529  419 VLCIAGPNVFSGYL-EAAHNKGLWL--------EDGWLNTGDLGRIDADGYFWLTGR----AKdliIRgGHNIDPAAIEE 485

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3020 RLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAE-YMIPSAFVTLDAFPLTPNGKLDRKALp 3098
Cdd:PRK07529  486 ALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASATEAELLAFARDHIAErAAVPKHVRILDALPKTAVGKIFKPAL- 564

                         570       580       590
                  ....*....|....*....|....*....|....*.
gi 641744967 3099 apdQSAMATRGYEApqgdlehALAQIWQTLLGVERV 3134
Cdd:PRK07529  565 ---RRDAIRRVLRA-------ALRDAGVEAEVVDVV 590
AACS cd05943
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ...
 486-957 5.50e-22

Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.


Pssm-ID: 341265 [Multi-domain]  Cd Length: 629  Bit Score: 103.89  E-value: 5.50e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  486 TPEAIAVLF-----EDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPA- 559
Cdd:cd05943    81 DADDPAAIYaaedgERTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVp 160

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  560 ---ERLAYIlddaAPVALLT------------QSAQVAQLNSTLPT----VLLDTPAAAACPDTNPVVQGLH-------- 612
Cdd:cd05943   161 gvlDRFGQI----EPKVLFAvdaytyngkrhdVREKVAELVKGLPSllavVVVPYTVAAGQPDLSKIAKALTledflatg 236

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  613 -----------AAHLAYVIYTSGSTGRPKGVMvaHRnvinlATGlhTLLalDHPSRIALNASI-----VFDASVKNWIQ- 675
Cdd:cd05943   237 aagelefeplpFDHPLYILYSSGTTGLPKCIV--HG-----AGG--TLL--QHLKEHILHCDLrpgdrLFYYTTCGWMMw 305

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  676 ------LLSGHTLVLV---PdaLRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLgsdpayQPA---------QVLIG 737
Cdd:cd05943   306 nwlvsgLAVGATIVLYdgsP--FYPDTNALWDLADEEGITVFGTSAKYLDALEKAGL------KPAethdlsslrTILST 377

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  738 G------------EAISPAVWsrLQSLSdtrfinvyGPTectvDATACVV--DRTQPL--PTIGKPLANTRLYILDAQDQ 801
Cdd:cd05943   378 GsplkpesfdyvyDHIKPDVL--LASIS--------GGT----DIISCFVggNPLLPVyrGEIQCRGLGMAVEAFDEEGK 443

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  802 PVpIGVTGELHIggagvARGYLHRP-----DLTAERFIPDPFSADPAarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGF 876
Cdd:cd05943   444 PV-WGEKGELVC-----TKPFPSMPvgfwnDPDGSRYRAAYFAKYPG--VWAHGDWIEITPRGGVVILGRSDGTLNPGGV 515

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  877 RIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPA---ALRQQLAASLADYMIPSAFVTLDALPLT 953
Cdd:cd05943   516 RIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDDElrkRIRSTIRSALSPRHVPAKIIAVPDIPRT 595


                  ....
gi 641744967  954 PNGK 957
Cdd:cd05943   596 LSGK 599
PRK05850 PRK05850
acyl-CoA synthetase; Validated
 483-852 5.81e-22

acyl-CoA synthetase; Validated


Pssm-ID: 235624 [Multi-domain]  Cd Length: 578  Bit Score: 103.48  E-value: 5.81e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEAIAVLFED---------QHLTYRELNRRANQLAHHLIALGVqPDDRVALCVERSLEMMVGLLGILKAGAAYVPM 553
Cdd:PRK05850   11 ASLQPDDAAFTFIDyeqdpagvaETLTWSQLYRRTLNVAEELRRHGS-TGDRAVILAPQGLEYIVAFLGALQAGLIAVPL 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  554 DPAYPA---ERLAYILDDAAPVALLTQSA---------QVAQLNSTLPTVLLDTPAAAACPDTNPVVQGLHAAhlAYVIY 621
Cdd:PRK05850   90 SVPQGGahdERVSAVLRDTSPSVVLTTSAvvddvteyvAPQPGQSAPPVIEVDLLDLDSPRGSDARPRDLPST--AYLQY 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  622 TSGSTGRPKGVMVAHRNVI-NLATGLHTLLAlDHPSRIALNASIVfdasvkNW--------------IQLLSGHTLVLV- 685
Cdd:PRK05850  168 TSGSTRTPAGVMVSHRNVIaNFEQLMSDYFG-DTGGVPPPDTTVV------SWlpfyhdmglvlgvcAPILGGCPAVLTs 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  686 PDA-LRADA---HQLWRYfaRHAvdlFDCTPvQLQWLLDAGLGSD---PAYQPAQVLI---GGEAISPAVWSRLQ----- 750
Cdd:PRK05850  241 PVAfLQRPArwmQLLASN--PHA---FSAAP-NFAFELAVRKTSDddmAGLDLGGVLGiisGSERVHPATLKRFAdrfap 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  751 -SLSDTRFINVYGPTECTV------------------------DATACVVDRTQPLPTIGKPLANTrLYILDAQDQ-PVP 804
Cdd:PRK05850  315 fNLRETAIRPSYGLAEATVyvatrepgqppesvrfdyeklsagHAKRCETGGGTPLVSYGSPRSPT-VRIVDPDTCiECP 393

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|.
gi 641744967  805 IGVTGELHIGGAGVARGYLHRPDLTAERF---IPDPFSADPAARIYKTGDL 852
Cdd:PRK05850  394 AGTVGEIWVHGDNVAAGYWQKPEETERTFgatLVDPSPGTPEGPWLRTGDL 444
PRK09274 PRK09274
peptide synthase; Provisional
 2620-3000 7.00e-22

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 103.05  E-value: 7.00e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEA----------SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPT 2689
Cdd:PRK09274   20 PDQLAVAVPGGrgadgklaydELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKAGAVPVLVDPG 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2690 YPVERLRYMLDDAKPVALISQ-SAHLG---------------------IMNGSLPVILLDDGETRPFDnEPDTPLDArkq 2747
Cdd:PRK09274  100 MGIKNLKQCLAEAQPDAFIGIpKAHLArrlfgwgkpsvrrlvtvggrlLWGGTTLATLLRDGAAAPFP-MADLAPDD--- 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2748 gltprhLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDV-------------LLGVTSLsfdisileifLP 2814
Cdd:PRK09274  176 ------MAAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIdlptfplfalfgpALGMTSV----------IP 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2815 --------LLNGARLILAtqaqaadaqqlamlIERHAVSFMQATPSTWRMLV---ELRDFALPPGFKALCGGEALP---- 2879
Cdd:PRK09274  240 dmdptrpaTVDPAKLFAA--------------IERYGVTNLFGSPALLERLGrygEANGIKLPSLRRVISAGAPVPiavi 305

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2880 ENLaTALLQKVTTLWNLYGPTE----TTIWST--LNGLTTPTPY-----IGHPIANTQIYILD---------AQGRVVPL 2939
Cdd:PRK09274  306 ERF-RAMLPPDAEILTPYGATEalpiSSIESReiLFATRAATDNgagicVGRPVDGVEVRIIAisdapipewDDALRLAT 384

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967 2940 GVAGEIHIAGAGVVRGYLGRPDLTAERFITDPfSGAPEARMyktGDLGRWLPDGTLEYLGR 3000
Cdd:PRK09274  385 GEIGEIVVAGPMVTRSYYNRPEATRLAKIPDG-QGDVWHRM---GDLGYLDAQGRLWFCGR 441
PRK09088 PRK09088
acyl-CoA synthetase; Validated
 2619-3097 7.23e-22

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 102.19  E-value: 7.23e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVV--FGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLR 2696
Cdd:PRK09088    8 QPQRLAAVdlALGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELD 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2697 YMLDDAKPvALISQSAHLGimNGSLPVILLDDgetrpFDNEPDTPLDARKQGLTPRHLAYVIYTSGSTGKPKGVMVEHAN 2776
Cdd:PRK09088   88 ALLQDAEP-RLLLGDDAVA--AGRTDVEDLAA-----FIASADALEPADTPSIPPERVSLILFTSGTSGQPKGVMLSERN 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2777 M----VNFLCSMRkepgIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPs 2852
Cdd:PRK09088  160 LqqtaHNFGVLGR----VDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGFEPKRTLGRLGDPALGITHYFCVP- 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2853 twRMLVELRDfalPPGFKA---------LCGGEALPENLATALLQKVTTLWNLYGPTETtiwSTLNGLTTPTPYI----- 2918
Cdd:PRK09088  235 --QMAQAFRA---QPGFDAaalrhltalFTGGAPHAAEDILGWLDDGIPMVDGFGMSEA---GTVFGMSVDCDVIrakag 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2919 --GHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLE 2996
Cdd:PRK09088  307 aaGIPTPTVQTRVVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAFTGDGW--------FRTGDIARRDADGFFW 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2997 YLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPS 3076
Cdd:PRK09088  379 VVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDLERIRSHLSTRLAKYKVPK 458

                         490       500
                  ....*....|....*....|.
gi 641744967 3077 AFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK09088  459 HLRLVDALPRTASGKLQKARL 479
PRK08633 PRK08633
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
 616-963 7.88e-22

2-acyl-glycerophospho-ethanolamine acyltransferase; Validated


Pssm-ID: 236315 [Multi-domain]  Cd Length: 1146  Bit Score: 104.62  E-value: 7.88e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  616 LAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIaLNASIVFDA---SVKNWIQLLSGHTLVLVPDALraD 692
Cdd:PRK08633  784 TATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVI-LSSLPFFHSfglTVTLWLPLLEGIKVVYHPDPT--D 860

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  693 AHQLWRYFARHAVDLFDCTPVQLQwlldaglgsdpAYQ------PAQ------VLIGGEAISPAVWSRLQSLSDTRFINV 760
Cdd:PRK08633  861 ALGIAKLVAKHRATILLGTPTFLR-----------LYLrnkklhPLMfaslrlVVAGAEKLKPEVADAFEEKFGIRILEG 929

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  761 YGPTECTVDATACVVDRTQP---------LPTIGKPLANTRLYILDAQD-QPVPIGVTGELHIGGAGVARGYLHRPDLTA 830
Cdd:PRK08633  930 YGATETSPVASVNLPDVLAAdfkrqtgskEGSVGMPLPGVAVRIVDPETfEELPPGEDGLILIGGPQVMKGYLGDPEKTA 1009

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  831 ErFIPDPfsadPAARIYKTGDLARWLPDGNI---DYLGRndFQiKVRGFRIEAGEIESRLLRCPGVQDAVVIAR--EDSP 905
Cdd:PRK08633 1010 E-VIKDI----DGIGWYVTGDKGHLDEDGFLtitDRYSR--FA-KIGGEMVPLGAVEEELAKALGGEEVVFAVTavPDEK 1081

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967  906 GDTRLVAyLCARPDAElhPAALRQQLAAS-LADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK08633 1082 KGEKLVV-LHTCGAED--VEELKRAIKESgLPNLWKPSRYFKVEALPLLGSGKLDLKGL 1137
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
 2753-3094 8.92e-22

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 99.02  E-value: 8.92e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2753 HLAYVIYTSGSTGKPKGVM-VEHANMVNFLCSmRKEPGIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAAD 2831
Cdd:cd17633     1 NPFYIGFTSGTTGLPKAYYrSERSWIESFVCN-EDLFNISGEDAILAPGPLSHSLFLYGAISALYLGGTFIGQRKFNPKS 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2832 AQQlamLIERHAVSFMQATPSTWRMLVeLRDFALPPGFKALCGGEALPENLATALLQ--KVTTLWNLYGPTETT-IWSTL 2908
Cdd:cd17633    80 WIR---KINQYNATVIYLVPTMLQALA-RTLEPESKIKSIFSSGQKLFESTKKKLKNifPKANLIEFYGTSELSfITYNF 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 NGLTTPTPYIGHPIANTQIYILDAQGRVVplgvaGEIHIAGAGVVRGYLGRPDLTAERFITdpfsgapearmykTGDLGR 2988
Cdd:cd17633   156 NQESRPPNSVGRPFPNVEIEIRNADGGEI-----GKIFVKSEMVFSGYVRGGFSNPDGWMS-------------VGDIGY 217

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2989 WLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAylLAQPDTVLEPaDLRQRLSEG 3068
Cdd:cd17633   218 VDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVA--LYSGDKLTYK-QLKRFLKQK 294

                         330       340
                  ....*....|....*....|....*.
gi 641744967 3069 VAEYMIPSAFVTLDAFPLTPNGKLDR 3094
Cdd:cd17633   295 LSRYEIPKKIIFVDSLPYTSSGKIAR 320
PRK06060 PRK06060
p-hydroxybenzoic acid--AMP ligase FadD22;
483-965 9.05e-22

p-hydroxybenzoic acid--AMP ligase FadD22;


Pssm-ID: 180374 [Multi-domain]  Cd Length: 705  Bit Score: 103.57  E-value: 9.05e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  483 AEQTPEA----IAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP 558
Cdd:PRK06060   11 AEQASEAgwydRPAFYAADVVTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELH 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  559 AERLAYILDDAAPVALLTQSA---QVAQLNSTLPTVLLDTPAAAACPDTNPVvqGLHAAhlAYVIYTSGSTGRPKGVMVA 635
Cdd:PRK06060   91 RDDHALAARNTEPALVVTSDAlrdRFQPSRVAEAAELMSEAARVAPGGYEPM--GGDAL--AYATYTSGTTGPPKAAIHR 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  636 HRNVINLATGLHTLLALDHPSRIAL-NASIVFDASVKN--WIQLLSGHTLVLVPDALRADAHQLWRyfARHAVDLFDCTP 712
Cdd:PRK06060  167 HADPLTFVDAMCRKALRLTPEDTGLcSARMYFAYGLGNsvWFPLATGGSAVINSAPVTPEAAAILS--ARFGPSVLYGVP 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  713 VQLQWLLDAgLGSDPAYQPAQVLIGGEAISPAVWSRL-QSLSDTRFINVYGPTECTVDATACVVDRTQPlPTIGKPLANT 791
Cdd:PRK06060  245 NFFARVIDS-CSPDSFRSLRCVVSAGEALELGLAERLmEFFGGIPILDGIGSTEVGQTFVSNRVDEWRL-GTLGRVLPPY 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  792 RLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPD--LTAERFIpdpfsadpaariyKTGDLARWLPDGNIDYLGRNDF 869
Cdd:PRK06060  323 EIRVVAPDGTTAGPGVEGDLWVRGPAIAKGYWNRPDspVANEGWL-------------DTRDRVCIDSDGWVTYRCRADD 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  870 QIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALR---QQLAASLADYMIPSAFVT 946
Cdd:PRK06060  390 TEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRdlhRGLLNRLSAFKVPHRFAV 469

                         490
                  ....*....|....*....
gi 641744967  947 LDALPLTPNGKLDRKALPA 965
Cdd:PRK06060  470 VDRLPRTPNGKLVRGALRK 488
COG4908 COG4908
Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General ...
 2164-2397 1.69e-21

Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General function prediction only];


Pssm-ID: 443936 [Multi-domain]  Cd Length: 243  Bit Score: 96.26  E-value: 1.69e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2164 LAPLQEGILFhhlLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCWQDlSQPVQVVWRQAILPINHFE 2243
Cdd:COG4908     1 LSPAQKRFLF---LEPGSNAYNIPAVLRLEGPLDVEALERALRELVRRHPALRTRFVEED-GEPVQRIDPDADLPLEVVD 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2244 PTS--PEDVLAQLQAH-TEPRTRRIDLSQAPLFRADIAHDpLQNEWLLALSFHHLISDHMTLALIVGEI----RLLLQHQ 2316
Cdd:COG4908    77 LSAlpEPEREAELEELvAEEASRPFDLARGPLLRAALIRL-GEDEHVLLLTIHHIISDGWSLGILLRELaalyAALLEGE 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2317 ADALPT-PLPYRNFIA---QTLSVPN-SAHEAYFRDKLADVDEPTA-PFGLLNVQGSGGDIHEARLVLDATLASAIRQQA 2390
Cdd:COG4908   156 PPPLPElPIQYADYAAwqrAWLQSEAlEKQLEYWRQQLAGAPPVLElPTDRPRPAVQTFRGATLSFTLPAELTEALKALA 235


                  ....*..
gi 641744967 2391 RHLGVSP 2397
Cdd:COG4908   236 KAHGATV 242
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
 2620-3097 3.13e-21

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 100.14  E-value: 3.13e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRyML 2699
Cdd:cd05929     6 LDRAQVFHQRRLLLLDVYSIALNRNARAAAAEGVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKSSRAPRAEAC-AI 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALisqsahlgimngslPVILLDDGETRPFDNEPDTPLDARKQGLTPRHLAY--VIYTSGSTGKPKGVMVEH--- 2774
Cdd:cd05929    85 IEIKAAAL--------------VCGLFTGGGALDGLEDYEAAEGGSPETPIEDEAAGwkMLYSGGTTGRPKGIKRGLpgg 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2775 --ANMVNFLCSMRKEPGiaQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQlamLIERHAVSFMQATPS 2852
Cdd:cd05929   151 ppDNDTLMAAALGFGPG--ADSVYLSPAPLYHAAPFRWSMTALFMGGTLVLMEKFDPEEFLR---LIERYRVTFAQFVPT 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2853 TW-RMLV---ELR---DFAlppGFKALCGGEA-----LPENLATALLQKVttlWNLYGPTE---TTIWSTLNGLTTPTPy 2917
Cdd:cd05929   226 MFvRLLKlpeAVRnayDLS---SLKRVIHAAApcppwVKEQWIDWGGPII---WEYYGGTEgqgLTIINGEEWLTHPGS- 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 IGHPIANtQIYILDAQGRVVPLGVAGEIHIAGAGVVRgYLGRPDLTAERFITDPFSgapearmyKTGDLGRWLPDGTLEY 2997
Cdd:cd05929   299 VGRAVLG-KVHILDEDGNEVPPGEIGEVYFANGPGFE-YTNDPEKTAAARNEGGWS--------TLGDVGYLDEDGYLYL 368

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2998 LGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPAD-------LRQRLSegva 3070
Cdd:cd05929   369 TDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPGADAGTALaeeliafLRDRLS---- 444

                         490       500
                  ....*....|....*....|....*..
gi 641744967 3071 EYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05929   445 RYKCPRSIEFVAELPRDDTGKLYRRLL 471
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
 2620-3099 3.63e-21

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 100.46  E-value: 3.63e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:PRK13383   49 PGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDALAAAL 128

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQSAHLGIMNGSLPVILLDDGETrpfdnepdtpLDARKQGLTPRHLA---YVIYTSGSTGKPKGVMVehan 2776
Cdd:PRK13383  129 RAHHISTVVADNEFAERIAGADDAVAVIDPAT----------AGAEESGGRPAVAApgrIVLLTSGTTGKPKGVPR---- 194

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2777 mvnflcSMRKEPGIAQEDVLLGVTSLSFDiSILEIFLPLLNGARLILATQAQAADAQqlaMLIERH--------AVSFMQ 2848
Cdd:PRK13383  195 ------APQLRSAVGVWVTILDRTRLRTG-SRISVAMPMFHGLGLGMLMLTIALGGT---VLTHRHfdaeaalaQASLHR 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2849 ATPSTWRMLVELRDFALPPGFKA----------LCGGEALPENLATALLQKV-TTLWNLYGPTETTIwstlNGLTTPTPY 2917
Cdd:PRK13383  265 ADAFTAVPVVLARILELPPRVRArnplpqlrvvMSSGDRLDPTLGQRFMDTYgDILYNGYGSTEVGI----GALATPADL 340

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 ------IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGagvvrgylgrpDLTAERFiTDPFSGAPEARMYKTGDLGRWLP 2991
Cdd:PRK13383  341 rdapetVGKPVAGCPVRILDRNNRPVGPRVTGRIFVGG-----------ELAGTRY-TDGGGKAVVDGMTSTGDMGYLDN 408

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2992 DGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAE 3071
Cdd:PRK13383  409 AGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSGVDAAQLRDYLKDRVSR 488

                         490       500
                  ....*....|....*....|....*...
gi 641744967 3072 YMIPSAFVTLDAFPLTPNGKLDRKALPA 3099
Cdd:PRK13383  489 FEQPRDINIVSSIPRNPTGKVLRKELPG 516
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
 2618-3094 5.33e-21

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 100.26  E-value: 5.33e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2618 CTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRY 2697
Cdd:PLN02860   19 LRGNAVVTISGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGIVAPLNYRWSFEEAKS 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2698 MLDDAKPVALIS----QSAHLGIMNGSLPV----ILLDDGETR--PFDNEPDTPLDARKQGLTPRHLAY---------VI 2758
Cdd:PLN02860   99 AMLLVRPVMLVTdetcSSWYEELQNDRLPSlmwqVFLESPSSSvfIFLNSFLTTEMLKQRALGTTELDYawapddavlIC 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2759 YTSGSTGKPKGVMVEHANMVnfLCSMRKEP--GIAQEDVLLGVTSL------SFDISIL-----EIFLPLLNgARLILAT 2825
Cdd:PLN02860  179 FTSGTTGRPKGVTISHSALI--VQSLAKIAivGYGEDDVYLHTAPLchigglSSALAMLmvgacHVLLPKFD-AKAALQA 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2826 qaqaadaqqlamlIERHAVSFMQATPSTWRMLVEL-RDFALPPGF----KALCGGEALPENL--ATALLQKVTTLWNLYG 2898
Cdd:PLN02860  256 -------------IKQHNVTSMITVPAMMADLISLtRKSMTWKVFpsvrKILNGGGSLSSRLlpDAKKLFPNAKLFSAYG 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2899 PTETTiwSTLNGLTTPTPYIGHPIANTQIYILDAQGRV--------------VPLGVA-------GEIHIAGAGVVRGYL 2957
Cdd:PLN02860  323 MTEAC--SSLTFMTLHDPTLESPKQTLQTVNQTKSSSVhqpqgvcvgkpaphVELKIGldessrvGRILTRGPHVMLGYW 400

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2958 GRPDLTAERFITDPFsgapearmYKTGDLGrWLPD-GTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIARE 3036
Cdd:PLN02860  401 GQNSETASVLSNDGW--------LDTGDIG-WIDKaGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVP 471

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967 3037 DSPGDKRLVAYL--------------LAQPDTVLEPADLRQRLSE-GVAEYMIPSAFVTL-DAFPLTPNGKLDR 3094
Cdd:PLN02860  472 DSRLTEMVVACVrlrdgwiwsdnekeNAKKNLTLSSETLRHHCREkNLSRFKIPKLFVQWrKPFPLTTTGKIRR 545
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
 482-958 7.57e-21

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 99.31  E-value: 7.57e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  482 QAEQTPEAIAVLFED--QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPA 559
Cdd:PRK13390    6 HAQIAPDRPAVIVAEtgEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTA 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  560 ERLAYILDDAAPVALLTQSAQ---VAQLNSTLPTVL-----------LDTPAAAACPDTNPVVQGlhaahlAYVIYTSGS 625
Cdd:PRK13390   86 PEADYIVGDSGARVLVASAALdglAAKVGADLPLRLsfggeidgfgsFEAALAGAGPRLTEQPCG------AVMLYSSGT 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  626 TGRPKGVM--VAHRNV-------INLATGLHTLLALDhpsrIALNASIVFDASVKNWIQLLS--GHTLVLvpdALRADAH 694
Cdd:PRK13390  160 TGFPKGIQpdLPGRDVdapgdpiVAIARAFYDISESD----IYYSSAPIYHAAPLRWCSMVHalGGTVVL---AKRFDAQ 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  695 QLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDATACV 774
Cdd:PRK13390  233 ATLGHVERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEYYSSTEAHGMTF 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  775 VDRTQPLP---TIGKPLANTrLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAErfipdpfSADPAARIYKT-G 850
Cdd:PRK13390  313 IDSPDWLAhpgSVGRSVLGD-LHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAA-------AQHPAHPFWTTvG 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  851 DLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSP-GD-TRLVAYLCA--RPDAELhPAA 926
Cdd:PRK13390  385 DLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEmGEqVKAVIQLVEgiRGSDEL-ARE 463

                         490       500       510
                  ....*....|....*....|....*....|..
gi 641744967  927 LRQQLAASLADYMIPSAFVTLDALPLTPNGKL 958
Cdd:PRK13390  464 LIDYTRSRIAHYKAPRSVEFVDELPRTPTGKL 495
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
 497-935 8.35e-21

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 99.08  E-value: 8.35e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  497 QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLT 576
Cdd:cd05932     5 VEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFV 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  577 -----QSAQVAQLNSTLPTVLLDTPAAAACPDTNPVVQGLH----------AAHLAYVIYTSGSTGRPKGVM-------- 633
Cdd:cd05932    85 gklddWKAMAPGVPEGLISISLPPPSAANCQYQWDDLIAQHppleerptrfPEQLATLIYTSGTTGQPKGVMltfgsfaw 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  634 VAHRNVINLATG----LHTLLALDH-PSRIALNAS-------IVFDASVKNWIQLLSGH--TLVL-VPdalradahQLWR 698
Cdd:cd05932   165 AAQAGIEHIGTEendrMLSYLPLAHvTERVFVEGGslyggvlVAFAESLDTFVEDVQRArpTLFFsVP--------RLWT 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  699 YFaRHAVdlFDCTPVQ-LQWLLD-------------AGLGSDpayqPAQVLIGGEA-ISPAV--WSRLQSLSdtrFINVY 761
Cdd:cd05932   237 KF-QQGV--QDKIPQQkLNLLLKipvvnslvkrkvlKGLGLD----QCRLAGCGSApVPPALleWYRSLGLN---ILEAY 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  762 GPTECTVDATACVVDRTQpLPTIGKPLantrlyildaQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsad 841
Cdd:cd05932   307 GMTENFAYSHLNYPGRDK-IGTVGNAG----------PGVEVRISEDGEILVRSPALMMGYYKDPEATAEAFTADGF--- 372

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  842 paariYKTGDLARWLPDGNIDYLGRNDFQIKV-RGFRIEAGEIESRLLRCPGVQDAVVIARedspGDTRLVAyLCArPDA 920
Cdd:cd05932   373 -----LRTGDKGELDADGNLTITGRVKDIFKTsKGKYVAPAPIENKLAEHDRVEMVCVIGS----GLPAPLA-LVV-LSE 441

                         490
                  ....*....|....*...
gi 641744967  921 ELHPAAL---RQQLAASL 935
Cdd:cd05932   442 EARLRADafaRAELEASL 459
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
 497-903 1.03e-20

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 98.83  E-value: 1.03e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  497 QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGaayVPMDPAYP---AERLAYILDDAAPVA 573
Cdd:cd17639     4 KYMSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQN---IPIVTVYAtlgEDALIHSLNETECSA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  574 LLTQSaqvaqlnstlptvlldtpaaaaCPDTnpvvqglhaahLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLaLD 653
Cdd:cd17639    81 IFTDG----------------------KPDD-----------LACIMYTSGSTGNPKGVMLTHGNLVAGIAGLGDRV-PE 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  654 HPSR----IA-LNASIVFDASVKNwIQLLSG------HTLVLVPDALR---ADAHQL-----------W-RYfaRHAV-- 705
Cdd:cd17639   127 LLGPddryLAyLPLAHIFELAAEN-VCLYRGgtigygSPRTLTDKSKRgckGDLTEFkptlmvgvpaiWdTI--RKGVla 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  706 ----------DLFD--------------CTPV---QLQWLLDAGLGSDPAYqpaqVLIGGEAISPavwsrlqslsDT-RF 757
Cdd:cd17639   204 klnpmgglkrTLFWtayqsklkalkegpGTPLldeLVFKKVRAALGGRLRY----MLSGGAPLSA----------DTqEF 269

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  758 INV--------YGPTEcTVdATACVVDRTQPLP-TIGKPLANTRLYILDAQ------DQPVPigvTGELHIGGAGVARGY 822
Cdd:cd17639   270 LNIvlcpviqgYGLTE-TC-AGGTVQDPGDLETgRVGPPLPCCEIKLVDWEeggystDKPPP---RGEILIRGPNVFKGY 344

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  823 LHRPDLTAERFIPDpfsadpaaRIYKTGDLARWLPDGNIDYLGRNDFQIKVR-GFRIEAGEIESRLLRCPGVQDAVVIAR 901
Cdd:cd17639   345 YKNPEKTKEAFDGD--------GWFHTGDIGEFHPDGTLKIIDRKKDLVKLQnGEYIALEKLESIYRSNPLVNNICVYAD 416


                  ..
gi 641744967  902 ED 903
Cdd:cd17639   417 PD 418
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
1525-2021 1.17e-20

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 99.36  E-value: 1.17e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1525 PHDALIHqLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLI-DLGVKPDDRIAICVERSLDMVIGLLAILKAGA 1603
Cdd:PRK08974   21 RYQSLVD-MFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQnGLGLKKGDRVALMMPNLLQYPIALFGILRAGM 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1604 AYVPLDPGYPAERLAYMLDDASPVALLTQAN--------------QRALLT--GD--------------------VPRIL 1647
Cdd:PRK08974  100 IVVNVNPLYTPRELEHQLNDSGAKAIVIVSNfahtlekvvfktpvKHVILTrmGDqlstakgtlvnfvvkyikrlVPKYH 179

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1648 LDTA----DFSHLSEDNPHV-PGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYR-LTPDDRVLQKTPFS- 1720
Cdd:PRK08974  180 LPDAisfrSALHKGRRMQYVkPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQAKAAYGpLLHPGKELVVTALPl 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1721 ---FDVSVWEFFWPLLYGARLVMARPdghKDAAYLAQLIER------TGITTLHfvpSMLQQFVQWADADCAcdSLRRVI 1791
Cdd:PRK08974  260 yhiFALTVNCLLFIELGGQNLLITNP---RDIPGFVKELKKypftaiTGVNTLF---NALLNNEEFQELDFS--SLKLSV 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1792 CSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTfwaCQPDDHRSFV-PIGRPIANTQLYILDTLGQPVPLGVAGELH 1870
Cdd:PRK08974  332 GGGMAVQQAVAERWVKLTGQYLLEGYGLTECSPLVS---VNPYDLDYYSgSIGLPVPSTEIKLVDDDGNEVPPGEPGELW 408

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1871 IGGVGVARGYLNRPDLTAErFIPDPFInqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPG 1950
Cdd:PRK08974  409 VKGPQVMLGYWQRPEATDE-VIKDGWL--------ATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMLHPK 479

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967 1951 VQEAVVVARE-DSPGDTRLVAYLCPQPGVTPDpaDLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK08974  480 VLEVAAVGVPsEVSGEAVKIFVVKKDPSLTEE--ELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRREL 549
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
 2752-3094 1.63e-20

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 95.79  E-value: 1.63e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2752 RHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEP-GIAQEDVLLGVTSLSFDISILEIFLPLL-NGARLILATQAQA 2829
Cdd:cd17635     1 EDPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGlNWVVGDVTYLPLPATHIGGLWWILTCLIhGGLCVTGGENTTY 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2830 ADAQQLamlIERHAVSFMQATPSTWRMLVELRDFALPPGFKALC---GGE-ALPENLATALLQKVTTLWNLYGPTETTIW 2905
Cdd:cd17635    81 KSLFKI---LTTNAVTTTCLVPTLLSKLVSELKSANATVPSLRLigyGGSrAIAADVRFIEATGLTNTAQVYGLSETGTA 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2906 STL---NGLTTPTPyIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmyK 2982
Cdd:cd17635   158 LCLptdDDSIEINA-VGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTAEVLIDGWV---------N 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2983 TGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQP-DTVLEPADL 3061
Cdd:cd17635   228 TGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASAeLDENAIRAL 307

                         330       340       350
                  ....*....|....*....|....*....|...
gi 641744967 3062 RQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDR 3094
Cdd:cd17635   308 KHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
 2630-3097 1.86e-20

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 98.68  E-value: 1.86e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2630 ASLSYDELNRRANRLAHHLISF-GVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDD--AKPVA 2706
Cdd:PRK05677   48 KTLTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDsgAKALV 127

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2707 LISQSAHLgiMNGSLP------VILLDDGETRPFD-------------------NEPD----TPLDARKQGLT------- 2750
Cdd:PRK05677  128 CLANMAHL--AEKVLPktgvkhVIVTEVADMLPPLkrllinavvkhvkkmvpayHLPQavkfNDALAKGAGQPvteanpq 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2751 PRHLAYVIYTSGSTGKPKGVMVEHANMVnflcsmrkePGIAQEDVLLGVtSLSFDISILEIFLPLLNGARLILATQAQaa 2830
Cdd:PRK05677  206 ADDVAVLQYTGGTTGVAKGAMLTHRNLV---------ANMLQCRALMGS-NLNEGCEILIAPLPLYHIYAFTFHCMAM-- 273

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2831 daqqlaMLIERHAV--SFMQATPStwrMLVELRDF------ALPPGFKALCGGE--------ALPENLA--TALLQKVTT 2892
Cdd:PRK05677  274 ------MLIGNHNIliSNPRDLPA---MVKELGKWkfsgfvGLNTLFVALCNNEafrkldfsALKLTLSggMALQLATAE 344

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2893 LWNL---------YGPTETTIWSTLNGLTTPTP-YIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDL 2962
Cdd:PRK05677  345 RWKEvtgcaicegYGMTETSPVVSVNPSQAIQVgTIGIPVPSTLCKVIDDDGNELPLGEVGELCVKGPQVMKGYWQRPEA 424

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2963 TAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDK 3042
Cdd:PRK05677  425 TDEILDSDGW--------LKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGE 496

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 3043 RLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK05677  497 AIKVFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRREL 551
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
 2757-3093 2.41e-20

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 95.06  E-value: 2.41e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2757 VIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLsFDISILEIFLP--LLNGARLILATQAQAADAQq 2834
Cdd:cd17636     5 AIYTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGTVFLNSGPL-FHIGTLMFTLAtfHAGGTNVFVRRVDAEEVLE- 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2835 lamLIERHAVSFMQATPSTWRMLVELRDfALPPGFKALCGGEALPE--NLATALlqkvTTLWN----LYGPTETTIWSTL 2908
Cdd:cd17636    83 ---LIEAERCTHAFLLPPTIDQIVELNA-DGLYDLSSLRSSPAAPEwnDMATVD----TSPWGrkpgGYGQTEVMGLATF 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 NGLTTPTPYI-GHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFitdpfsgapEARMYKTGDLG 2987
Cdd:cd17636   155 AALGGGAIGGaGRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRT---------RGGWHHTNDLG 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2988 RWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSE 3067
Cdd:cd17636   226 RREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGASVTEAELIEHCRA 305

                         330       340
                  ....*....|....*....|....*.
gi 641744967 3068 GVAEYMIPSAFVTLDAFPLTPNGKLD 3093
Cdd:cd17636   306 RIASYKKPKSVEFADALPRTAGGADD 331
PRK08180 PRK08180
feruloyl-CoA synthase; Reviewed
 467-936 2.72e-20

feruloyl-CoA synthase; Reviewed


Pssm-ID: 236175 [Multi-domain]  Cd Length: 614  Bit Score: 98.41  E-value: 2.72e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  467 ADFPREMLiqQRFEAQAEQTPEAIAVLFED-----QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLL 541
Cdd:PRK08180   35 GDYPRRLT--DRLVHWAQEAPDRVFLAERGadggwRRLTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEHALLAL 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  542 GILKAGAAYVPMDPAYPA-----ERLAYIL----------DDAAPVA-------------LLTQSAQVAQLNSTLPTvLL 593
Cdd:PRK08180  113 AAMYAGVPYAPVSPAYSLvsqdfGKLRHVLelltpglvfaDDGAAFAralaavvpadvevVAVRGAVPGRAATPFAA-LL 191

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  594 DTPAAAAcpdtnpvVQGLHAA----HLAYVIYTSGSTGRPKGVMVAHRNV-INLATGLHTL--LALDHPSRI-------- 658
Cdd:PRK08180  192 ATPPTAA-------VDAAHAAvgpdTIAKFLFTSGSTGLPKAVINTHRMLcANQQMLAQTFpfLAEEPPVLVdwlpwnht 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  659 ---ALNASIV------------------FDASVKNwIQLLSGHTLVLVP-------DALRADAHQLWRYFARhaVDLFdc 710
Cdd:PRK08180  265 fggNHNLGIVlynggtlyiddgkptpggFDETLRN-LREISPTVYFNVPkgwemlvPALERDAALRRRFFSR--LKLL-- 339

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  711 tpvqlqwlldaglgsdpAYqpaqvliGGEAISPAVWSRLQSLS------DTRFINVYGPTECTVDATACvvdrTQPLPT- 783
Cdd:PRK08180  340 -----------------FY-------AGAALSQDVWDRLDRVAeatcgeRIRMMTGLGMTETAPSATFT----TGPLSRa 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  784 --IGKPLANTRLYIldaqdqpVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWL----P 857
Cdd:PRK08180  392 gnIGLPAPGCEVKL-------VPVGGKLEVRVKGPNVTPGYWRAPELTAEAFDEEGY--------YRSGDAVRFVdpadP 456

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  858 DGNIDYLGR--NDFQIkVRGFRIEAGEIESRLLR--CPGVQDAVVIA--RE--------DSPGDTRLVAYLCARPDAE-L 922
Cdd:PRK08180  457 ERGLMFDGRiaEDFKL-SSGTWVSVGPLRARAVSagAPLVQDVVITGhdRDeigllvfpNLDACRRLAGLLADASLAEvL 535

                         570
                  ....*....|....
gi 641744967  923 HPAALRQQLAASLA 936
Cdd:PRK08180  536 AHPAVRAAFRERLA 549
PRK05850 PRK05850
acyl-CoA synthetase; Validated
 1533-1924 2.78e-20

acyl-CoA synthetase; Validated


Pssm-ID: 235624 [Multi-domain]  Cd Length: 578  Bit Score: 98.09  E-value: 2.78e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1533 LVEDQAARTPDTTAVLFED---------QHLTYDALNRRANQLAHHLIDLGVkPDDRIAICVERSLDMVIGLLAILKAGA 1603
Cdd:PRK05850    6 LLRERASLQPDDAAFTFIDyeqdpagvaETLTWSQLYRRTLNVAEELRRHGS-TGDRAVILAPQGLEYIVAFLGALQAGL 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1604 AYVPLD---PGYPAERLAYMLDDASPVALLTQAN--------QRALLTGDVPRIL-LDTADFShlSEDNPHVPGLDAHHL 1671
Cdd:PRK05850   85 IAVPLSvpqGGAHDERVSAVLRDTSPSVVLTTSAvvddvteyVAPQPGQSAPPVIeVDLLDLD--SPRGSDARPRDLPST 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1672 AYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTY------RLTPDDRVLQKTPFSFDVS-VWEFFWPLLYGARLVMARPD 1744
Cdd:PRK05850  163 AYLQYTSGSTRTPAGVMVSHRNVIANFEQLMSDYfgdtggVPPPDTTVVSWLPFYHDMGlVLGVCAPILGGCPAVLTSPV 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1745 G--HKDAAYLAQLIERTGittlhfVPSMLQQFV------QWADADCACDSLRRV--ICSG-EAL-PAELqQRF---FARF 1809
Cdd:PRK05850  243 AflQRPARWMQLLASNPH------AFSAAPNFAfelavrKTSDDDMAGLDLGGVlgIISGsERVhPATL-KRFadrFAPF 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1810 NAQLHNL---YGPTEAAIDVT------------FWA----------CQPDDHRSFVPIGRPIANTqLYILD--TLGQpVP 1862
Cdd:PRK05850  316 NLRETAIrpsYGLAEATVYVAtrepgqppesvrFDYeklsaghakrCETGGGTPLVSYGSPRSPT-VRIVDpdTCIE-CP 393

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1863 LGVAGELHIGGVGVARGYLNRPDLTAERF---IPDPFINQPGARLYKTGDLArWLPDGSLEYLGR 1924
Cdd:PRK05850  394 AGTVGEIWVHGDNVAAGYWQKPEETERTFgatLVDPSPGTPEGPWLRTGDLG-FISEGELFIVGR 457
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
 1566-2018 3.02e-20

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 97.95  E-value: 3.02e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1566 LAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQANQRA----LLTG 1641
Cdd:PLN02860   45 LAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGIVAPLNYRWSFEEAKSAMLLVRPVMLVTDETCSSwyeeLQND 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1642 DVP----RILLDT-------ADFSHLSEDNPHVPGLDAHHLAY---------VIYTSGSTGKPKGVMNSHRALCnrlvwM 1701
Cdd:PLN02860  125 RLPslmwQVFLESpsssvfiFLNSFLTTEMLKQRALGTTELDYawapddavlICFTSGTTGRPKGVTISHSALI-----V 199

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLT-----PDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMArPDGHKDAAYlaQLIERTGITTLHFVPSMLQQFVQ 1776
Cdd:PLN02860  200 QSLAKIAivgygEDDVYLHTAPLCHIGGLSSALAMLMVGACHVLL-PKFDAKAAL--QAIKQHNVTSMITVPAMMADLIS 276

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1777 WA---DADCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNLYGPTEAAIDVTFWA-----CQPDDHRSFVPIGRPIA 1847
Cdd:PLN02860  277 LTrksMTWKVFPSVRKILNGGGSLSSRLLPDAKKLFpNAKLFSAYGMTEACSSLTFMTlhdptLESPKQTLQTVNQTKSS 356

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1848 NTQLYILDTLGQPVP-----LGVAGELHIG-----GVGVARGYLNRPDLTAERFIPDPFINqpgarlykTGDLArWLPD- 1916
Cdd:PLN02860  357 SVHQPQGVCVGKPAPhvelkIGLDESSRVGriltrGPHVMLGYWGQNSETASVLSNDGWLD--------TGDIG-WIDKa 427

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1917 GSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLG------ 1990
Cdd:PLN02860  428 GNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACVRLRDGWIWSDNEKENAKKnltlss 507

                         490       500       510
                  ....*....|....*....|....*....|....*...
gi 641744967 1991 ---------QHLAEYMVPGAFVTL-DAFPLTPNGKLDR 2018
Cdd:PLN02860  508 etlrhhcreKNLSRFKIPKLFVQWrKPFPLTTTGKIRR 545
PRK12582 PRK12582
acyl-CoA synthetase; Provisional
 470-937 3.49e-20

acyl-CoA synthetase; Provisional


Pssm-ID: 237144 [Multi-domain]  Cd Length: 624  Bit Score: 98.19  E-value: 3.49e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  470 PREMLIQQRFEAQAEQTPEAIAVLFEDQH------LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGI 543
Cdd:PRK12582   46 PYPRSIPHLLAKWAAEAPDRPWLAQREPGhgqwrkVTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAA 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  544 LKAGAAYVPMDPAYPA-----ERLAYILDDAAPVALLTQSAQ-----VAQLNSTLPTVLLDTPAA-----------AACP 602
Cdd:PRK12582  126 MQAGVPAAPVSPAYSLmshdhAKLKHLFDLVKPRVVFAQSGApfaraLAALDLLDVTVVHVTGPGegiasiafadlAATP 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  603 DTNPVVQ---GLHAAHLAYVIYTSGSTGRPKGVMVAHR---NVINLATGLHTLLALDHPSrIALN---------ASIVFD 667
Cdd:PRK12582  206 PTAAVAAaiaAITPDTVAKYLFTSGSTGMPKAVINTQRmmcANIAMQEQLRPREPDPPPP-VSLDwmpwnhtmgGNANFN 284

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  668 ASvknwiqLLSGHTLVLvpDALRADAHQlwryFARHAVDLFDCTPV------QLQWLLDAGLGSDPAYQPA------QVL 735
Cdd:PRK12582  285 GL------LWGGGTLYI--DDGKPLPGM----FEETIRNLREISPTvygnvpAGYAMLAEAMEKDDALRRSffknlrLMA 352

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  736 IGGEAISPAVWSRLQSLSDTR------FINVYGPTEcTVDATACVVDRTQPLPTIGKPLANTRLYIldaqdqpVPIGVTG 809
Cdd:PRK12582  353 YGGATLSDDLYERMQALAVRTtghripFYTGYGATE-TAPTTTGTHWDTERVGLIGLPLPGVELKL-------APVGDKY 424

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  810 ELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWL----PDGNIDYLGR--NDFQIKVrGFRIEAGEI 883
Cdd:PRK12582  425 EVRVKGPNVTPGYHKDPELTAAAFDEEGF--------YRLGDAARFVdpddPEKGLIFDGRvaEDFKLST-GTWVSVGTL 495

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967  884 ESRLLRC--PGVQDAVViAREDSPGDTRLV-------AYLCARPDAELHPAALRQQLAASLAD 937
Cdd:PRK12582  496 RPDAVAAcsPVIHDAVV-AGQDRAFIGLLAwpnpaacRQLAGDPDAAPEDVVKHPAVLAILRE 557
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 2632-3044 4.06e-20

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 96.10  E-value: 4.06e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPV-ALISQ 2710
Cdd:cd05974     1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRVDRGGAVyAAVDE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2711 SAHlgimngslpvillddgetrpfdnePDTPLdarkqgltprhLAYviYTSGSTGKPKGVMVEHANM-VNFLCSMRkEPG 2789
Cdd:cd05974    81 NTH------------------------ADDPM-----------LLY--FTSGTTSKPKLVEHTHRSYpVGHLSTMY-WIG 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2790 IAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVE--LRDFALPP 2867
Cdd:cd05974   123 LKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLFNYARFDAKRVLAALVRYGVTTLCAPPTVWRMLIQqdLASFDVKL 202

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2868 gfKALCG-GEAL-PEnlataLLQKVTTLWNL-----YGPTETTIW-STLNGLTTPTPYIGHPIANTQIYILDAQGRVVPL 2939
Cdd:cd05974   203 --REVVGaGEPLnPE-----VIEQVRRAWGLtirdgYGQTETTALvGNSPGQPVKAGSMGRPLPGYRVALLDPDGAPATE 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2940 G----VAGEIHiaGAGVVRGYLGRPDLTAerfitdpfsGAPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELG 3015
Cdd:cd05974   276 GevalDLGDTR--PVGLMKGYAGDPDKTA---------HAMRGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPF 344

                         410       420
                  ....*....|....*....|....*....
gi 641744967 3016 EIETRLARCHGVHDAVVIAredSPGDKRL 3044
Cdd:cd05974   345 ELESVLIEHPAVAEAAVVP---SPDPVRL 370
PRK12582 PRK12582
acyl-CoA synthetase; Provisional
 1525-2001 4.77e-20

acyl-CoA synthetase; Provisional


Pssm-ID: 237144 [Multi-domain]  Cd Length: 624  Bit Score: 97.81  E-value: 4.77e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1525 PHDALIHQLVEDQAARTPDTTAVLFEDQH------LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAI 1598
Cdd:PRK12582   46 PYPRSIPHLLAKWAAEAPDRPWLAQREPGhgqwrkVTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAA 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1599 LKAGAAYVPLDPGYPA-----ERLAYMLDDASPVALLTQAN---QRALLT---GDVPRILLDTA----DFSHLSE--DNP 1661
Cdd:PRK12582  126 MQAGVPAAPVSPAYSLmshdhAKLKHLFDLVKPRVVFAQSGapfARALAAldlLDVTVVHVTGPgegiASIAFADlaATP 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1662 HVPGLDAHH-------LAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVlqktPFSFDVSVWEF------ 1728
Cdd:PRK12582  206 PTAAVAAAIaaitpdtVAKYLFTSGSTGMPKAVINTQRMMCANIAMQEQLRPREPDPPP----PVSLDWMPWNHtmggna 281

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1729 -FWPLLY-GARLVM--ARPdghkdaayLAQLIERTgITTLH--------FVP---SMLQQFVQWADADCAC--DSLRRVI 1791
Cdd:PRK12582  282 nFNGLLWgGGTLYIddGKP--------LPGMFEET-IRNLReisptvygNVPagyAMLAEAMEKDDALRRSffKNLRLMA 352

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1792 CSGEALPAELQQRFFARFNAQ------LHNLYGPTEAA--IDVTFWacqpdDHRSFVPIGRPIANTQLYIldtlgqpVPL 1863
Cdd:PRK12582  353 YGGATLSDDLYERMQALAVRTtghripFYTGYGATETAptTTGTHW-----DTERVGLIGLPLPGVELKL-------APV 420

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1864 GVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWL----PDGSLEYLGR--NDFqvKL-RGFRI 1936
Cdd:PRK12582  421 GDKYEVRVKGPNVTPGYHKDPELTAAAFDEEGF--------YRLGDAARFVdpddPEKGLIFDGRvaEDF--KLsTGTWV 490

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1937 ELGEIEARLMQ-CPGVQEAVVVAREDSPGDTRLV-------AYLCPQPGVTPDP--------ADLRQQLGQHLAEymVPG 2000
Cdd:PRK12582  491 SVGTLRPDAVAaCSPVIHDAVVAGQDRAFIGLLAwpnpaacRQLAGDPDAAPEDvvkhpavlAILREGLSAHNAE--AGG 568


                  .
gi 641744967 2001 A 2001
Cdd:PRK12582  569 S 569
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
 2632-3097 6.41e-20

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 96.58  E-value: 6.41e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLD--PTYP--------VERLRYMLDd 2701
Cdd:cd05906    40 QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLTvpPTYDepnarlrkLRHIWQLLG- 118

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2702 aKPVALISQSAHLGIMnGSLPVILLDDGETRPFDNEPDTPLDARKQGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFL 2781
Cdd:cd05906   119 -SPVVLTDAELVAEFA-GLETLSGLPGIRVLSIEELLDTAADHDLPQSRPDDLALLMLTSGSTGFPKAVPLTHRNILARS 196

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2782 CSMRKEPGIAQEDVLLG------VTSLSFdISILEIFLpllnGARLILATQAQAADAQQLAM-LIERHAVSFMQATPSTW 2854
Cdd:cd05906   197 AGKIQHNGLTPQDVFLNwvpldhVGGLVE-LHLRAVYL----GCQQVHVPTEEILADPLRWLdLIDRYRVTITWAPNFAF 271

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2855 RMLVEL------RDFALPPGFKALCGGEALPENLATALLQ-------KVTTLWNLYGPTET---TIWS---TLNGLTTPT 2915
Cdd:cd05906   272 ALLNDLleeiedGTWDLSSLRYLVNAGEAVVAKTIRRLLRllepyglPPDAIRPAFGMTETcsgVIYSrsfPTYDHSQAL 351

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2916 PY--IGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGrWLPDG 2993
Cdd:cd05906   352 EFvsLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPEANAEAFTEDGW--------FRTGDLG-FLDNG 422

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2994 TLEYLGRNDFQVKVRGFRIELGEIETRLARCHGV---HDAVVIAREDSPGDKRLVAYLL---AQPDTVLEPAD-LRQRLS 3066
Cdd:cd05906   423 NLTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVepsFTAAFAVRDPGAETEELAIFFVpeyDLQDALSETLRaIRSVVS 502

                         490       500       510
                  ....*....|....*....|....*....|....
gi 641744967 3067 E--GVA-EYMIPsafVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05906   503 RevGVSpAYLIP---LPKEEIPKTSLGKIQRSKL 533
COG4908 COG4908
Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General ...
 31-258 9.81e-20

Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General function prediction only];


Pssm-ID: 443936 [Multi-domain]  Cd Length: 243  Bit Score: 91.25  E-value: 9.81e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   31 LAPLQEGILFhyqLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGlHQPVQVVWRQAPLTVNTLT 110
Cdd:COG4908     1 LSPAQKRFLF---LEPGSNAYNIPAVLRLEGPLDVEALERALRELVRRHPALRTRFVEED-GEPVQRIDPDADLPLEVVD 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  111 TTS---SDTVPAQLRAATDPSNHRLNLSNAPLLSAT---TAHDpvcgEWLLSLSIHHLISDHITQALIIDEI----RLLL 180
Cdd:COG4908    77 LSAlpePEREAELEELVAEEASRPFDLARGPLLRAAlirLGED----EHVLLLTIHHIISDGWSLGILLRELaalyAALL 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  181 EDRPEALP-KPLPYRNFIA----QILSVPLSEHEQYFRNRLADIDTPTA-PFDLVDVQGNGEDITEARLSLDSSLADALR 254
Cdd:COG4908   153 EGEPPPLPeLPIQYADYAAwqraWLQSEALEKQLEYWRQQLAGAPPVLElPTDRPRPAVQTFRGATLSFTLPAELTEALK 232


                  ....
gi 641744967  255 RQAR 258
Cdd:COG4908   233 ALAK 236
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
1519-2021 1.16e-19

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 96.10  E-value: 1.16e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1519 ATEADFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLI-DLGVKPDDRIAICVERSLDMVIGLLA 1597
Cdd:PRK08751   16 AAEIDLEQFRTVAEVFATSVAKFADRPAYHSFGKTITYREADQLVEQFAAYLLgELQLKKGDRVALMMPNCLQYPIATFG 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1598 ILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQAN-----QRALLTGDVPRI----LLDTADFSH------------- 1655
Cdd:PRK08751   96 VLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNfgttvQQVIADTPVKQVittgLGDMLGFPKaalvnfvvkyvkk 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1656 ----------------LSEDNPH-VPGLDAHH--LAYVIYTSGSTGKPKGVMNSHRALCNRLV----WMQNTYRLTP-DD 1711
Cdd:PRK08751  176 lvpeyringairfreaLALGRKHsMPTLQIEPddIAFLQYTGGTTGVAKGAMLTHRNLVANMQqahqWLAGTGKLEEgCE 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1712 RVLQKTPFS--FDVSVWEFFWPLLYGARLVMARPdghKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADAD-CACDSLR 1788
Cdd:PRK08751  256 VVITALPLYhiFALTANGLVFMKIGGCNHLISNP---RDMPGFVKELKKTRFTAFTGVNTLFNGLLNTPGFDqIDFSSLK 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1789 RVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTFWACQPDDHRSfvPIGRPIANTQLYILDTLGQPVPLGVAGE 1868
Cdd:PRK08751  333 MTLGGGMAVQRSVAERWKQVTGLTLVEAYGLTETSPAACINPLTLKEYNG--SIGLPIPSTDACIKDDAGTVLAIGEIGE 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1869 LHIGGVGVARGYLNRPDLTAERFIPDPFINqpgarlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQC 1948
Cdd:PRK08751  411 LCIKGPQVMKGYWKRPEETAKVMDADGWLH--------TGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMM 482

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1949 PGVQE--AVVVAREDSpGDTRLVAYLCPQPGVTPDpaDLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK08751  483 PGVLEvaAVGVPDEKS-GEIVKVVIVKKDPALTAE--DVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRREL 554
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
 2607-3099 1.27e-19

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 96.41  E-value: 1.27e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2607 LIHELFEAQVACTPDAIAVVF-GE----ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGG 2681
Cdd:cd05968    62 IVEQLLDKWLADTRTRPALRWeGEdgtsRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGG 141

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2682 AYVPLDPTYPVERLRYMLDDAKPVALISQSahlGIMNGSLPVILLDDGET------------------RPFDNEPDTPL- 2742
Cdd:cd05968   142 IVVPIFSGFGKEAAATRLQDAEAKALITAD---GFTRRGREVNLKEEADKacaqcptvekvvvvrhlgNDFTPAKGRDLs 218

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2743 --------DARKQGLTPRHLAYVIYTSGSTGKPKGVMVEHANM-------VNFLCSMRkepgiaQEDVLLGVTSLSFDIS 2807
Cdd:cd05968   219 ydeeketaGDGAERTESEDPLMIIYTSGTTGKPKGTVHVHAGFplkaaqdMYFQFDLK------PGDLLTWFTDLGWMMG 292

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2808 ILEIFLPLLNGARLILATQAQAADAQQLAM-LIERHAVSFMQATPSTWRMLVELRDFALPPGFKA---LCGGEALPENL- 2882
Cdd:cd05968   293 PWLIFGGLILGATMVLYDGAPDHPKADRLWrMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSslrVLGSTGEPWNPe 372

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2883 ------ATALLQKVTTLwNLYGPTETTIWSTLNGLTTPTPYIGH--PIANTQIYILDAQGRVVPlGVAGEIHIAGA--GV 2952
Cdd:cd05968   373 pwnwlfETVGKGRNPII-NYSGGTEISGGILGNVLIKPIKPSSFngPVPGMKADVLDESGKPAR-PEVGELVLLAPwpGM 450

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2953 VRGYLGRPDltaeRFITDPFSGAPEARMYktGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVV 3032
Cdd:cd05968   451 TRGFWRDED----RYLETYWSRFDNVWVH--GDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAA 524

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967 3033 IAREDSPGDKRLVAYLLAQPDtVLEPADLRQRLSEGVAEYM----IPSAFVTLDAFPLTPNGKLDRKALPA 3099
Cdd:cd05968   525 IGVPHPVKGEAIVCFVVLKPG-VTPTEALAEELMERVADELgkplSPERILFVKDLPKTRNAKVMRRVIRA 594
PRK09192 PRK09192
fatty acyl-AMP ligase;
499-960 1.52e-19

fatty acyl-AMP ligase;


Pssm-ID: 236403 [Multi-domain]  Cd Length: 579  Bit Score: 95.84  E-value: 1.52e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMD-PAYPAERLAYI------LDDAAP 571
Cdd:PRK09192   50 LPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGLVPVPLPlPMGFGGRESYIaqlrgmLASAQP 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  572 VALLT---------QSAQVAQLNSTLPTVLLDTPAAAACPDTNPVvqglhAAHLAYVIYTSGSTGRPKGVMVAHRNVI-N 641
Cdd:PRK09192  130 AAIITpdellpwvnEATHGNPLLHVLSHAWFKALPEADVALPRPT-----PDDIAYLQYSSGSTRFPRGVIITHRALMaN 204

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  642 LATGLHTLLALDHPSRIAlnasivfdasvkNWIQLLsgHTLVLVPDALRADAHQL------WRYFARHAvdlfdctpvqL 715
Cdd:PRK09192  205 LRAISHDGLKVRPGDRCV------------SWLPFY--HDMGLVGFLLTPVATQLsvdylpTRDFARRP----------L 260

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  716 QWL--------------------------------LD------AGLGSDPAyqPAQVLIG-GEAISPAvwsrlqSLSDTR 756
Cdd:PRK09192  261 QWLdlisrnrgtisysppfgyelcarrvnskdlaeLDlscwrvAGIGADMI--RPDVLHQfAEAFAPA------GFDDKA 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  757 FINVYGPTECT----------------VDATAC--------VVDRTQPLPTI---GKPLANTRLYILDAQDQPVPIGVTG 809
Cdd:PRK09192  333 FMPSYGLAEATlavsfsplgsgivveeVDRDRLeyqgkavaPGAETRRVRTFvncGKALPGHEIEIRNEAGMPLPERVVG 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  810 ELHIGGAGVARGYLHRPDlTAERFIPDPFsadpaariYKTGDLArWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLR 889
Cdd:PRK09192  413 HICVRGPSLMSGYFRDEE-SQDVLAADGW--------LDTGDLG-YLLDGYLYITGRAKDLIIINGRNIWPQDIEWIAEQ 482

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967  890 CPGVQ--DAVVIAREDSPGDTRLVAYLCARPDAELHpAALRQQLAASLadYMIP--SAFVTL---DALPLTPNGKLDR 960
Cdd:PRK09192  483 EPELRsgDAAAFSIAQENGEKIVLLVQCRISDEERR-GQLIHALAALV--RSEFgvEAAVELvppHSLPRTSSGKLSR 557
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
617-963 1.84e-19

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 93.19  E-value: 1.84e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  617 AYVIYTSGSTGRPKGVMVAHRNVINLATGLHT--------LLALDhPSRIA----LNASIVfdASVKNWIQLLSGHtlvL 684
Cdd:PRK07824   38 ALVVATSGTTGTPKGAMLTAAALTASADATHDrlggpgqwLLALP-AHHIAglqvLVRSVI--AGSEPVELDVSAG---F 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  685 VPDALRADAHQL---WRYFArhavdlfdCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRLQSLsDTRFINVY 761
Cdd:PRK07824  112 DPTALPRAVAELgggRRYTS--------LVPMQLAKALDDPAATAALAELDAVLVGGGPAPAPVLDAAAAA-GINVVRTY 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  762 GPTECtvdATACVVDrtqplptiGKPLANTRLYILDaqdqpvpigvtGELHIGGAGVARGYLHRPDltaerfiPDPFsAD 841
Cdd:PRK07824  183 GMSET---SGGCVYD--------GVPLDGVRVRVED-----------GRIALGGPTLAKGYRNPVD-------PDPF-AE 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  842 PAAriYKTGDLARwLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAE 921
Cdd:PRK07824  233 PGW--FRTDDLGA-LDDGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDGGPA 309

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 641744967  922 LHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK07824  310 PTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRAL 351
AACS cd05943
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ...
 1540-2015 2.11e-19

Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.


Pssm-ID: 341265 [Multi-domain]  Cd Length: 629  Bit Score: 95.80  E-value: 2.11e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1540 RTPDTTAVLF-----EDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPA 1614
Cdd:cd05943    80 ADADDPAAIYaaedgERTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGV 159

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1615 ERLAYMLDDASPVALLT-----------------QANQRALLTGD----VPRILLD-------TADFSHLSEDNPHVPG- 1665
Cdd:cd05943   160 PGVLDRFGQIEPKVLFAvdaytyngkrhdvrekvAELVKGLPSLLavvvVPYTVAAgqpdlskIAKALTLEDFLATGAAg 239

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1666 ------LDAHHLAYVIYTSGSTGKPKGVMNSH----------RALCNRLvwmqntyrlTPDDRVlqktpFSFDVSVWeFF 1729
Cdd:cd05943   240 elefepLPFDHPLYILYSSGTTGLPKCIVHGAggtllqhlkeHILHCDL---------RPGDRL-----FYYTTCGW-MM 304

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1730 W-----PLLYGARLVMArpDG---HKDAAYLAQLIERTGITTLHFVPSMLQ-----QFVQWADADCacDSLRRVICSGEA 1796
Cdd:cd05943   305 WnwlvsGLAVGATIVLY--DGspfYPDTNALWDLADEEGITVFGTSAKYLDalekaGLKPAETHDL--SSLRTILSTGSP 380

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1797 LPAElQQRFF---ARFNAQLHNLYGPTEaaIDVTFWACQPDdhrsfVPIGR-----PIANTQLYILDTLGQPVPlGVAGE 1868
Cdd:cd05943   381 LKPE-SFDYVydhIKPDVLLASISGGTD--IISCFVGGNPL-----LPVYRgeiqcRGLGMAVEAFDEEGKPVW-GEKGE 451

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1869 LHIggvgvARGYLNRP-----DLTAERFIPDPFINQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEA 1943
Cdd:cd05943   452 LVC-----TKPFPSMPvgfwnDPDGSRYRAAYFAKYPG--VWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYR 524

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 1944 RLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPAdLRQQLGQHLAEYM----VPGAFVTLDAFPLTPNGK 2015
Cdd:cd05943   525 VVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDDE-LRKRIRSTIRSALsprhVPAKIIAVPDIPRTLSGK 599
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
 2599-3097 2.16e-19

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 95.27  E-value: 2.16e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2599 DADIPRHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISF-GVRPDERVAICVERGLDMVVGLLGIL 2677
Cdd:PRK12492   17 TIDLAAYKSVVEVFERSCKKFADRPAFSNLGVTLSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNVLQYPIAVFGAL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2678 KAGGAYVPLDPTYPVERLRYMLDDAKPVALIsqsaHLGIMNGSLPVILLDDG-----ETRPFDNEP-------DTPLDAR 2745
Cdd:PRK12492   97 RAGLIVVNTNPLYTAREMRHQFKDSGARALV----YLNMFGKLVQEVLPDTGieyliEAKMGDLLPaakgwlvNTVVDKV 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2746 KQGLTPRHL----------------------------AYVIYTSGSTGKPKGVMVEH----ANMVNFLCSMRKEPGIAQE 2793
Cdd:PRK12492  173 KKMVPAYHLpqavpfkqalrqgrglslkpvpvglddiAVLQYTGGTTGLAKGAMLTHgnlvANMLQVRACLSQLGPDGQP 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2794 DVLLGVTSLSFDISILEIFLPLLNGARLILATQAQaadaqqlaMLIE--RHAVSFMQATpSTWR---------MLVELRD 2862
Cdd:PRK12492  253 LMKEGQEVMIAPLPLYHIYAFTANCMCMMVSGNHN--------VLITnpRDIPGFIKEL-GKWRfsallglntLFVALMD 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2863 FalpPGFKAL---------CGGEALPEnlATA-LLQKVT--TLWNLYGPTETTIWSTLNglttptPY--------IGHPI 2922
Cdd:PRK12492  324 H---PGFKDLdfsalkltnSGGTALVK--ATAeRWEQLTgcTIVEGYGLTETSPVASTN------PYgelarlgtVGIPV 392

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2923 ANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRND 3002
Cdd:PRK12492  393 PGTALKVIDDDGNELPLGERGELCIKGPQVMKGYWQQPEATAEALDAEGW--------FKTGDIAVIDPDGFVRIVDRKK 464

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3003 FQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQpDTVLEPADLRQRLSEGVAEYMIPSAFVTLD 3082
Cdd:PRK12492  465 DLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVAR-DPGLSVEELKAYCKENFTGYKVPKHIVLRD 543

                         570
                  ....*....|....*
gi 641744967 3083 AFPLTPNGKLDRKAL 3097
Cdd:PRK12492  544 SLPMTPVGKILRREL 558
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
 2633-3097 2.26e-19

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 95.20  E-value: 2.26e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2633 SYDELNRRANRLAHHLISFGVRPDERVAIC---VERGLDMVVGLLGIlkaGGAYVPLDPTYPVERLRYMLDDAKPVALIS 2709
Cdd:PRK06018   41 TYAQIHDRALKVSQALDRDGIKLGDRVATIawnTWRHLEAWYGIMGI---GAICHTVNPRLFPEQIAWIINHAEDRVVIT 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2710 QSAHLGIMN---GSLP----VILLDDGETRPfdnepDTPL---------------DARKQGLTPRHLAYVIYTSGSTGKP 2767
Cdd:PRK06018  118 DLTFVPILEkiaDKLPsverYVVLTDAAHMP-----QTTLknavayeewiaeadgDFAWKTFDENTAAGMCYTSGTTGDP 192

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2768 KGVMVEH-ANMVNFLCSMRKEP-GIAQEDVLLGVTSL----SFDISileiFLPLLNGARLILATQAQAADAQQLamLIER 2841
Cdd:PRK06018  193 KGVLYSHrSNVLHALMANNGDAlGTSAADTMLPVVPLfhanSWGIA----FSAPSMGTKLVMPGAKLDGASVYE--LLDT 266

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2842 HAVSFMQATPSTWRMLV---ELRDFALPPGFKALCGGEALPENLATALLQKVTTLWNLYGPTETTIWSTLNGLTTPTPYI 2918
Cdd:PRK06018  267 EKVTFTAGVPTVWLMLLqymEKEGLKLPHLKMVVCGGSAMPRSMIKAFEDMGVEVRHAWGMTEMSPLGTLAALKPPFSKL 346

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2919 ------------GHPIANTQIYILDAQGRVVPL-GVA-GEIHIAGAGVVRGYLGrpdltAERFITDpfsgapEARMYKTG 2984
Cdd:PRK06018  347 pgdarldvlqkqGYPPFGVEMKITDDAGKELPWdGKTfGRLKVRGPAVAAAYYR-----VDGEILD------DDGFFDTG 415

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2985 DLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETrLARCH-GVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQ 3063
Cdd:PRK06018  416 DVATIDAYGYMRITDRSKDVIKSGGEWISSIDLEN-LAVGHpKVAEAAVIGVYHPKWDERPLLIVQLKPGETATREEILK 494

                         490       500       510
                  ....*....|....*....|....*....|....
gi 641744967 3064 RLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK06018  495 YMDGKIAKWWMPDDVAFVDAIPHTATGKILKTAL 528
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
 2617-3091 2.30e-19

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 94.67  E-value: 2.30e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2617 ACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLR 2696
Cdd:cd12118    15 AVYPDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEEIA 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2697 YMLDDAKPVALISQSAHLGIMngslpviLLDDGETRPfdnEPDTPLDARkQGLTprhlayVIYTSGSTGKPKGVMVEH-- 2774
Cdd:cd12118    95 FILRHSEAKVLFVDREFEYED-------LLAEGDPDF---EWIPPADEW-DPIA------LNYTSGTTGRPKGVVYHHrg 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2775 ------ANMVNFlcSMRKEP------------------GIAQedvlLGVTSlsfdisileIFLPLLNgARLILAtqaqaa 2830
Cdd:cd12118   158 aylnalANILEW--EMKQHPvylwtlpmfhcngwcfpwTVAA----VGGTN---------VCLRKVD-AKAIYD------ 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2831 daqqlamLIERHAVSFMQATPSTWRMLVELRDFA---LPPGFKALCGGEALPEnlatALLQKVTTLW----NLYGPTETT 2903
Cdd:cd12118   216 -------LIEKHKVTHFCGAPTVLNMLANAPPSDarpLPHRVHVMTAGAPPPA----AVLAKMEELGfdvtHVYGLTETY 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2904 ------IWS-TLNGLTTPTPYI-----GHP-IANTQIYILDAQG-RVVPL-GV-AGEIHIAGAGVVRGYLGRPDLTAERF 2967
Cdd:cd12118   285 gpatvcAWKpEWDELPTEERARlkarqGVRyVGLEEVDVLDPETmKPVPRdGKtIGEIVFRGNIVMKGYLKNPEATAEAF 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2968 itdpfsgapEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAY 3047
Cdd:cd12118   365 ---------RGGWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAF 435

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 641744967 3048 LLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDaFPLTPNGK 3091
Cdd:cd12118   436 VELKEGAKVTEEEIIAFCREHLAGFMVPKTVVFGE-LPKTSTGK 478
PrpE cd05967
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ...
 2595-3097 2.46e-19

Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.


Pssm-ID: 341271 [Multi-domain]  Cd Length: 617  Bit Score: 95.46  E-value: 2.46e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2595 FNATDadipRHALihelfeaqvACTPDAIAVVF------GEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLD 2668
Cdd:cd05967    53 YNALD----RHVE---------AGRGDQIALIYdspvtgTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPE 119

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2669 MVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQSAhlGI-----------------MNGSLP--VILLDDG 2729
Cdd:cd05967   120 AAIAMLACARIGAIHSVVFGGFAAKELASRIDDAKPKLIVTASC--GIepgkvvpykplldkaleLSGHKPhhVLVLNRP 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2730 ETRPFDNEPDTPLD-------ARKQGLTP---RHLAYVIYTSGSTGKPKGVMVE---HANMVNFlcSMRKEPGIAQEDVL 2796
Cdd:cd05967   198 QVPADLTKPGRDLDwsellakAEPVDCVPvaaTDPLYILYTSGTTGKPKGVVRDnggHAVALNW--SMRNIYGIKPGDVW 275

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2797 LGVTSLSFDISILEI-FLPLLNGARLILATQAQAADAQQLAM--LIERHAVSFMQATPSTWRML-------VELRDFALP 2866
Cdd:cd05967   276 WAASDVGWVVGHSYIvYGPLLHGATTVLYEGKPVGTPDPGAFwrVIEKYQVNALFTAPTAIRAIrkedpdgKYIKKYDLS 355

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2867 pGFKAL-CGGEAL-PENLATAllQKVTTL------WNlygpTET-----TIWSTLNGLTTPTPYIGHPIANTQIYILDAQ 2933
Cdd:cd05967   356 -SLRTLfLAGERLdPPTLEWA--ENTLGVpvidhwWQ----TETgwpitANPVGLEPLPIKAGSPGKPVPGYQVQVLDED 428

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2934 GRVVPLGVAGEIHIAGA---GVVRGYLGRPdltaERFITDPFSGAPEarMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGF 3010
Cdd:cd05967   429 GEPVGPNELGNIVIKLPlppGCLLTLWKND----ERFKKLYLSKFPG--YYDTGDAGYKDEDGYLFIMGRTDDVINVAGH 502

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3011 RIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIP-SAF---VTLDAFPL 3086
Cdd:cd05967   503 RLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVKITAEELEKELVALVREQIGPvAAFrlvIFVKRLPK 582

                         570
                  ....*....|.
gi 641744967 3087 TPNGKLDRKAL 3097
Cdd:cd05967   583 TRSGKILRRTL 593
FATP_FACS cd05940
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ...
 1551-1999 7.19e-19

Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341263 [Multi-domain]  Cd Length: 449  Bit Score: 92.80  E-value: 7.19e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1551 DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAayvpldpgypaerlaymlddaspVALL 1630
Cdd:cd05940     1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGA-----------------------VAAL 57

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1631 TQANQRalltGDVPRILLDTADFSHLSEDnphvpgldahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPD 1710
Cdd:cd05940    58 INYNLR----GESLAHCLNVSSAKHLVVD-----------AALYIYTSGTTGLPKAAIISHRRAWRGGAFFAGSGGALPS 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1711 DRVLQKTP-FSFDVSVWEFFWPLLYGARLVMAR--------PDGHKDAAYLAQLIERTgITTLHFVPsmlqqfvqwadad 1781
Cdd:cd05940   123 DVLYTCLPlYHSTALIVGWSACLASGATLVIRKkfsasnfwDDIRKYQATIFQYIGEL-CRYLLNQP------------- 188

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1782 cACDSLR----RVICsGEALPAELQQRFFARFN-AQLHNLYGPTEAAIDVTFWACQPDD---HRSFVPIGRPIANTQlYI 1853
Cdd:cd05940   189 -PKPTERkhkvRMIF-GNGLRPDIWEEFKERFGvPRIAEFYAATEGNSGFINFFGKPGAigrNPSLLRKVAPLALVK-YD 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1854 LDTlGQP----------VPLGVAGEL--HIGGVGVARGYLNrPDLTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEY 1921
Cdd:cd05940   266 LES-GEPirdaegrcikVPRGEPGLLisRINPLEPFDGYTD-PAATEKKILRDVF--KKGDAWFNTGDLMRLDGEGFWYF 341

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 1922 LGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRL-VAYLCPQPGVTPDPADLRQQLGQHLAEYMVP 1999
Cdd:cd05940   342 VDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQVPGTDGRAgMAAIVLQPNEEFDLSALAAHLEKNLPGYARP 420
PLN03102 PLN03102
acyl-activating enzyme; Provisional
 1542-2045 8.02e-19

acyl-activating enzyme; Provisional


Pssm-ID: 215576 [Multi-domain]  Cd Length: 579  Bit Score: 93.55  E-value: 8.02e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:PLN03102   28 PNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAIL 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVAL--------LTQANQRALLTGDV---PRILL------------DTADFSHLSEDNPHVPGL--------DAHH 1670
Cdd:PLN03102  108 RHAKPKILfvdrsfepLAREVLHLLSSEDSnlnLPVIFiheidfpkrpssEELDYECLIQRGEPTPSLvarmfriqDEHD 187

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1671 LAYVIYTSGSTGKPKGVMNSHR-----ALCNRLVWMQNTYRLtpddrVLQKTPFsFDVSVWEFFWPL-LYGARLVMARpd 1744
Cdd:PLN03102  188 PISLNYTSGTTADPKGVVISHRgaylsTLSAIIGWEMGTCPV-----YLWTLPM-FHCNGWTFTWGTaARGGTSVCMR-- 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1745 gHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLR-RVICSGEALPAELQQRfFARFNAQLHNLYGPTEAA 1823
Cdd:PLN03102  260 -HVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRSGPvHVLTGGSPPPAALVKK-VQRLGFQVMHAYGLTEAT 337

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1824 IDVTFwaCQPDDHRSFVPIGRPIANTQLYILDTLG------------QPVPLG--VAGELHIGGVGVARGYLNRPDLTAE 1889
Cdd:PLN03102  338 GPVLF--CEWQDEWNRLPENQQMELKARQGVSILGladvdvknketqESVPRDgkTMGEIVIKGSSIMKGYLKNPKATSE 415

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1890 RFiPDPFINqpgarlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLV 1969
Cdd:PLN03102  416 AF-KHGWLN--------TGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPC 486

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1970 AYLCPQPGVTPDPA----------DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDR-------KALPAPDQSAVATR 2032
Cdd:PLN03102  487 AFVVLEKGETTKEDrvdklvtrerDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKpklrdiaKGLVVEDEDNVIKK 566

                         570
                  ....*....|...
gi 641744967 2033 DYEAPQGEVETAL 2045
Cdd:PLN03102  567 VHQRPVEHFSSRL 579
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 2754-3097 8.62e-19

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 91.00  E-value: 8.62e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2754 LAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLsFDI--SILEIFLPLLNGARLILATQAQAAD 2831
Cdd:cd05944     4 VAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPL-FHVngSVVTLLTPLASGAHVVLAGPAGYRN 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2832 AQQLA---MLIERHAVSFMQATPSTWRMLVELRDFALPPGFK-ALCGGEALPENLATALLQKV-TTLWNLYGPTETTIWS 2906
Cdd:cd05944    83 PGLFDnfwKLVERYRITSLSTVPTVYAALLQVPVNADISSLRfAMSGAAPLPVELRARFEDATgLPVVEGYGLTEATCLV 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2907 TLNGLTTPTPyIGH-----PIANTQIYILDAQGRVV-PLGV--AGEIHIAGAGVVRGYLgRPDLTAERFITDPFsgapea 2978
Cdd:cd05944   163 AVNPPDGPKR-PGSvglrlPYARVRIKVLDGVGRLLrDCAPdeVGEICVAGPGVFGGYL-YTEGNKNAFVADGW------ 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2979 rmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEP 3058
Cdd:cd05944   235 --LNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVVEE 312

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|
gi 641744967 3059 ADLRQRLSEGVAEY-MIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05944   313 EELLAWARDHVPERaAVPKHIEVLEELPVTAVGKVFKPAL 352
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
 619-960 9.42e-19

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 90.78  E-value: 9.42e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  619 VIYTSGSTGRPKGVMVAHR-----------NVINLATG--LHTLLALDHPSRIALNASIVFDA-------------SVKN 672
Cdd:cd17635     6 VIFTSGTTGEPKAVLLANKtffavpdilqkEGLNWVVGdvTYLPLPATHIGGLWWILTCLIHGglcvtggenttykSLFK 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  673 WIQLLSGHTLVLVPDAlradahqlWRYFARHAVDLFDCTPvQLQWlldaglgsdpayqpaqVLIGGEAISPAVWSRLQSL 752
Cdd:cd17635    86 ILTTNAVTTTCLVPTL--------LSKLVSELKSANATVP-SLRL----------------IGYGGSRAIAADVRFIEAT 140

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  753 SDTRFINVYGPTECTvdaTACVVDRTQPLPTI---GKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLT 829
Cdd:cd17635   141 GLTNTAQVYGLSETG---TALCLPTDDDSIEInavGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERT 217

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  830 AERFIPDPFsadpaariyKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTR 909
Cdd:cd17635   218 AEVLIDGWV---------NTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGEL 288

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967  910 LVAYLCARP-DAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDR 960
Cdd:cd17635   289 VGLAVVASAeLDENAIRALKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
 1530-2023 9.73e-19

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 93.17  E-value: 9.73e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1530 IHQLVEDQAarTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGvKPDDR--IAICVERSLDMVIGLLAILKAGAAYVP 1607
Cdd:PRK13388    5 IAQLLRDRA--GDDTIAVRYGDRTWTWREVLAEAAARAAALIALA-DPDRPlhVGVLLGNTPEMLFWLAAAALGGYVLVG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1608 LDPGYPAERLAYMLDDASPVALLTQANQRALLTG----DVPRILLDTADFSHL----SEDNPHVPgLDAHHLAYVIYTSG 1679
Cdd:PRK13388   82 LNTTRRGAALAADIRRADCQLLVTDAEHRPLLDGldlpGVRVLDVDTPAYAELvaaaGALTPHRE-VDAMDPFMLIFTSG 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1680 STGKPKGVMNSH-------RALCNRlvwmqntYRLTPDDRVLQKTPFSFDVSVWEFFWPLL-YGARlvMARPDGHKDAAY 1751
Cdd:PRK13388  161 TTGAPKAVRCSHgrlafagRALTER-------FGLTRDDVCYVSMPLFHSNAVMAGWAPAVaSGAA--VALPAKFSASGF 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1752 LAQlIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSGEALPaELQQRFFARFNAQLHNLYGPTEAAIDVTfwac 1831
Cdd:PRK13388  232 LDD-VRRYGATYFNYVGKPLAYILATPERPDDADNPLRVAFGNEASP-RDIAEFSRRFGCQVEDGYGSSEGAVIVV---- 305

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1832 qPDDHRSFVPIGRPIANTQLYILDTLgqpVPLGVA---------------GEL-HIGGVGVARGYLNRPDLTAERFipdp 1895
Cdd:PRK13388  306 -REPGTPPGSIGRGAPGVAIYNPETL---TECAVArfdahgallnadeaiGELvNTAGAGFFEGYYNNPEATAERM---- 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1896 finqpgaR--LYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLC 1973
Cdd:PRK13388  378 -------RhgMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALV 450

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 641744967 1974 PQPGVTPDPADLRQQLG--QHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK13388  451 LRDGATFDPDAFAAFLAaqPDLGTKAWPRYVRIAADLPSTATNKVLKRELIA 502
AcpA COG3433
Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites ...
 1840-2114 1.01e-18

Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442659 [Multi-domain]  Cd Length: 295  Bit Score: 89.81  E-value: 1.01e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1840 VPIGRPIANTQLYILDTLGQPVPLGVAGelhiGGVGVARGYLNRPDLTAERFIPDPFINQPGARLYKTGDLARWLPDGSL 1919
Cdd:COG3433    18 PVIPPAIVQARALLLIVDLQGYFGGFGG----EGGLLGAGLLLRIRLLAAAARAPFIPVPYPAQPGRQADDLRLLLRRGL 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1920 EYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQ--PGVTPDPADLRQQLGQHLAEYM 1997
Cdd:COG3433    94 GPGGGLERLVQQVVIRAERGEEEELLLVLRAAAVVRVAVLAALRGAGVGLLLIVGAvaALDGLAAAAALAALDKVPPDVV 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1998 VPGAFVTLDAFPLTPNGKLDRKALPAPDQSAVATRDYEAPQGE---VETALAAVWQDLLGLT--RVGRHDHFFALGGHSL 2072
Cdd:COG3433   174 AASAVVALDALLLLALKVVARAAPALAAAEALLAAASPAPALEtalTEEELRADVAELLGVDpeEIDPDDNLFDLGLDSI 253

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|..
gi 641744967 2073 LIVSLIERLRRAGLALDVRGVFSTPVLSDMAQAILAHQDKPA 2114
Cdd:COG3433   254 RLMQLVERWRKAGLDVSFADLAEHPTLAAWWALLAAAQAAAA 295
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
 503-963 1.08e-18

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 92.44  E-value: 1.08e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  503 ELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPaERLAYILDDAAPVALLTQSAQVA 582
Cdd:cd05929    22 VYSIALNRNARAAAAEGVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKSSRAP-RAEACAIIEIKAAALVCGLFTGG 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  583 QLNSTLPTvllDTPAAAACPDT--NPVVQGlhaahlAYVIYTSGSTGRPKGVMVAHR-NVINLATGLHTLLALDHPS-RI 658
Cdd:cd05929   101 GALDGLED---YEAAEGGSPETpiEDEAAG------WKMLYSGGTTGRPKGIKRGLPgGPPDNDTLMAAALGFGPGAdSV 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  659 ALNASIVFDASVKNW--IQLLSGHTLVLVPdalRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPA--QV 734
Cdd:cd05929   172 YLSPAPLYHAAPFRWsmTALFMGGTLVLME---KFDPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEAVRNAYDLSslKR 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  735 LIGGEA-ISPAVWSRLQSLSDTRFINVYGPTECTvdaTACVVDRTQPLP---TIGKPLANtRLYILDAQDQPVPIGVTGE 810
Cdd:cd05929   249 VIHAAApCPPWVKEQWIDWGGPIIWEYYGGTEGQ---GLTIINGEEWLThpgSVGRAVLG-KVHILDEDGNEVPPGEIGE 324

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  811 LHIGGAGvARGYLHRPDLTAERFIPDPFSAdpaariykTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRC 890
Cdd:cd05929   325 VYFANGP-GFEYTNDPEKTAAARNEGGWST--------LGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAH 395

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967  891 PGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPA---ALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05929   396 PKVLDAAVVGVPDEELGQRVHAVVQPAPGADAGTAlaeELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLL 471
starter-C_NRPS cd19533
Starter Condensation domains, found in the first module of nonribosomal peptide synthetases ...
 29-345 1.49e-18

Starter Condensation domains, found in the first module of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. While standard C-domains catalyze peptide bond formation between two amino acids, an initial, ('starter') C-domain may instead acylate an amino acid with a fatty acid. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380456 [Multi-domain]  Cd Length: 419  Bit Score: 91.28  E-value: 1.49e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   29 YPLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICWQGLhQPVQVV--WRQAPLTV 106
Cdd:cd19533     2 LPLTSAQRGVWFAEQLDPEGSIYNLAEYLEITGPVDLAVLERALRQVIAEAETLRLRFTEEEG-EPYQWIdpYTPVPIRH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  107 ntlTTTSSDTVP--AQLRAATDPSNHRLNLSNAPLLSAT--TAHDpvcGEWLLSLSIHHLISDHITQAL----IIDEIRL 178
Cdd:cd19533    81 ---IDLSGDPDPegAAQQWMQEDLRKPLPLDNDPLFRHAlfTLGD---NRHFWYQRVHHIVMDGFSFALfgqrVAEIYTA 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  179 LLEDRPealPKPLPYRNFIAQILS-VPLSEHEQYFRNRLADIDTPTAPFDLVDVQGNGE----DITEARLSLDSSLADAL 253
Cdd:cd19533   155 LLKGRP---APPAPFGSFLDLVEEeQAYRQSERFERDRAFWTEQFEDLPEPVSLARRAPgrslAFLRRTAELPPELTRTL 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  254 RRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLqgNLGADRVMGMFINTLPLRVSLR-ERSVHDVVQATSH 332
Cdd:cd19533   232 LEAAEAHGASWPSFFIALVAAYLHRLTGANDVVLGVPVMGRL--GAAARQTPGMVANTLPLRLTVDpQQTFAELVAQVSR 309

                         330
                  ....*....|...
gi 641744967  333 ELMMLLAHEQAPL 345
Cdd:cd19533   310 ELRSLLRHQRYRY 322
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
2597-3097 1.79e-18

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 92.25  E-value: 1.79e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2597 ATDADIPRHALIHELFEAQVACTPDAIAVV-FGEaSLSYDELNRRANRLAHHLIS-FGVRPDERVAICVERGLDMVVGLL 2674
Cdd:PRK08751   16 AAEIDLEQFRTVAEVFATSVAKFADRPAYHsFGK-TITYREADQLVEQFAAYLLGeLQLKKGDRVALMMPNCLQYPIATF 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2675 GILKAGGAYVPLDPTYPVERLRYMLDDAKPVAL------------------ISQ--SAHLGIMNGSLPVILLDDGETRPF 2734
Cdd:PRK08751   95 GVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLvvidnfgttvqqviadtpVKQviTTGLGDMLGFPKAALVNFVVKYVK 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2735 DNEPDTPL-------DARKQG---------LTPRHLAYVIYTSGSTGKPKGVMVEHANMVnflCSMRKE------PGIAQ 2792
Cdd:PRK08751  175 KLVPEYRIngairfrEALALGrkhsmptlqIEPDDIAFLQYTGGTTGVAKGAMLTHRNLV---ANMQQAhqwlagTGKLE 251

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2793 EDVLLGVTSLSfdisILEIFLPLLNGarLILatqaqaadaqqlaMLIE--RHAVSFMQATPSTWRMLVELRDFALP---- 2866
Cdd:PRK08751  252 EGCEVVITALP----LYHIFALTANG--LVF-------------MKIGgcNHLISNPRDMPGFVKELKKTRFTAFTgvnt 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2867 --------PGFK---------ALCGGEALPENLATALlQKVT--TLWNLYGPTETTIWSTLNGLTTP--TPYIGHPIANT 2925
Cdd:PRK08751  313 lfngllntPGFDqidfsslkmTLGGGMAVQRSVAERW-KQVTglTLVEAYGLTETSPAACINPLTLKeyNGSIGLPIPST 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2926 QIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQV 3005
Cdd:PRK08751  392 DACIKDDAGTVLAIGEIGELCIKGPQVMKGYWKRPEETAKVMDADGW--------LHTGDIARMDEQGFVYIVDRKKDMI 463

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3006 KVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKrLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFP 3085
Cdd:PRK08751  464 LVSGFNVYPNEIEDVIAMMPGVLEVAAVGVPDEKSGE-IVKVVIVKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELP 542

                         570
                  ....*....|..
gi 641744967 3086 LTPNGKLDRKAL 3097
Cdd:PRK08751  543 KTNVGKILRREL 554
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
2601-3097 2.14e-18

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 92.04  E-value: 2.14e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2601 DIPRHALIHELFEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLIS-FGVRPDERVAICVERGLDMVVGLLGILKA 2679
Cdd:PRK08974   18 NPDRYQSLVDMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNgLGLKKGDRVALMMPNLLQYPIALFGILRA 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2680 GGAYVPLDPTYPVERLRYMLDD--AKPVALISQSAHL---GIMNGSLP-VILLDDGETRPFD------------------ 2735
Cdd:PRK08974   98 GMIVVNVNPLYTPRELEHQLNDsgAKAIVIVSNFAHTlekVVFKTPVKhVILTRMGDQLSTAkgtlvnfvvkyikrlvpk 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2736 -NEPD--TPLDARKQG---------LTPRHLAYVIYTSGSTGKPKGVMVEHANMV-NFL-CSMRKEPGIaQEDVLLGVTS 2801
Cdd:PRK08974  178 yHLPDaiSFRSALHKGrrmqyvkpeLVPEDLAFLQYTGGTTGVAKGAMLTHRNMLaNLEqAKAAYGPLL-HPGKELVVTA 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2802 LSfdisILEIFLPLLN-------GARLILATQAQAADAQQLAMliERHAVSFMQATPSTWRMLVELRDFAlPPGFKAL-- 2872
Cdd:PRK08974  257 LP----LYHIFALTVNcllfielGGQNLLITNPRDIPGFVKEL--KKYPFTAITGVNTLFNALLNNEEFQ-ELDFSSLkl 329

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2873 --CGGEALPENLATALlQKVT--TLWNLYGPTETTiwstlnGLTTPTPY--------IGHPIANTQIYILDAQGRVVPLG 2940
Cdd:PRK08974  330 svGGGMAVQQAVAERW-VKLTgqYLLEGYGLTECS------PLVSVNPYdldyysgsIGLPVPSTEIKLVDDDGNEVPPG 402

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2941 VAGEIHIAGAGVVRGYLGRPDLTAErFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETR 3020
Cdd:PRK08974  403 EPGELWVKGPQVMLGYWQRPEATDE-VIKDGW--------LATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDV 473

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 3021 LARCHGVHDAVVIARE-DSPGDkrLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK08974  474 VMLHPKVLEVAAVGVPsEVSGE--AVKIFVVKKDPSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRREL 549
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 499-933 2.15e-18

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 91.09  E-value: 2.15e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPmdpaypaerlayilddaaPVALLTQs 578
Cdd:cd05974     1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIP------------------ATTLLTP- 61

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 aqvAQLNSTLptvllDTPAAAACPdtnpVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRN--VINLAT----GLHtllal 652
Cdd:cd05974    62 ---DDLRDRV-----DRGGAVYAA----VDENTHADDPMLLYFTSGTTSKPKLVEHTHRSypVGHLSTmywiGLK----- 124

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  653 dhPSRIALNASIVFDA--SVKNWIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSdPAYQ 730
Cdd:cd05974   125 --PGDVHWNISSPGWAkhAWSCFFAPWNAGATVFLFNYARFDAKRVLAALVRYGVTTLCAPPTVWRMLIQQDLAS-FDVK 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  731 PAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTEctvdaTACVVDRT--QPLPT--IGKPLANTRLYILDAQDQPVPIG 806
Cdd:cd05974   202 LREVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQTE-----TTALVGNSpgQPVKAgsMGRPLPGYRVALLDPDGAPATEG 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  807 VTGeLHIGG---AGVARGYLHRPDLTAERFipdpfsadpAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEI 883
Cdd:cd05974   277 EVA-LDLGDtrpVGLMKGYAGDPDKTAHAM---------RGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFEL 346

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  884 ESRLLRCPGVQDAVVIAredSPGDTRLV---AYLCARPDAELHPAA-------LRQQLAA 933
Cdd:cd05974   347 ESVLIEHPAVAEAAVVP---SPDPVRLSvpkAFIVLRAGYEPSPETaleifrfSRERLAP 403
PRK07769 PRK07769
long-chain-fatty-acid--CoA ligase; Validated
 1523-1743 2.63e-18

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 181109 [Multi-domain]  Cd Length: 631  Bit Score: 92.10  E-value: 2.63e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1523 DFPHDALIHQLVEDQAARTPDTTAVLFED---------QHLTYDALNRRANQLAHHLIDLGvKPDDRIAICVERSLDMVI 1593
Cdd:PRK07769   16 RFPPNTNLVRHVERWAKVRGDKLAYRFLDfsterdgvaRDLTWSQFGARNRAVGARLQQVT-KPGDRVAILAPQNLDYLI 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1594 GLLAILKAGAAYVPL-DPGYP--AERLAYMLDDASPVALLTQANQ--------RALLTGDVPRIL-LDTADFSHLSEDNP 1661
Cdd:PRK07769   95 AFFGALYAGRIAVPLfDPAEPghVGRLHAVLDDCTPSAILTTTDSaegvrkffRARPAKERPRVIaVDAVPDEVGATWVP 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1662 HVPGLDAhhLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMA 1741
Cdd:PRK07769  175 PEANEDT--IAYLQYTSGSTRIPAGVQITHLNLPTNVLQVIDALEGQEGDRGVSWLPFFHDMGLITVLLPALLGHYITFM 252


                  ..
gi 641744967 1742 RP 1743
Cdd:PRK07769  253 SP 254
PRK12476 PRK12476
putative fatty-acid--CoA ligase; Provisional
 499-962 3.25e-18

putative fatty-acid--CoA ligase; Provisional


Pssm-ID: 171527 [Multi-domain]  Cd Length: 612  Bit Score: 91.73  E-value: 3.25e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALgVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPM-DPAYP--AERLAYILDDAAPVALL 575
Cdd:PRK12476   69 LTWTQLGVRLRAVGARLQQV-AGPGDRVAILAPQGIDYVAGFFAAIKAGTIAVPLfAPELPghAERLDTALRDAEPTVVL 147

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  576 TQSA---QVAQLNSTLPT------VLLDTPAAAACPDTNPVvqGLHAAHLAYVIYTSGSTGRPKGVMVAHRNV-INLatg 645
Cdd:PRK12476  148 TTTAaaeAVEGFLRNLPRlrrprvIAIDAIPDSAGESFVPV--ELDTDDVSHLQYTSGSTRPPVGVEITHRAVgTNL--- 222

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  646 LHTLLALDHPSRIALNAS------------IVFDASVKNWIQLLSGHTLVLVP----DALRADAHQlWRYFArhAVDLFd 709
Cdd:PRK12476  223 VQMILSIDLLDRNTHGVSwlplyhdmglsmIGFPAVYGGHSTLMSPTAFVRRPqrwiKALSEGSRT-GRVVT--AAPNF- 298

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  710 ctpvQLQWLLDAGL---GSDPAYQPAQVLIGGEAISPAVWSRLQS------LSDTRFINVYGPTECTV-------DATAC 773
Cdd:PRK12476  299 ----AYEWAAQRGLpaeGDDIDLSNVVLIIGSEPVSIDAVTTFNKafapygLPRTAFKPSYGIAEATLfvatiapDAEPS 374

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  774 VV--DRTQ-------PLP----------TIGKPLANTRLYILDA-QDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERF 833
Cdd:PRK12476  375 VVylDREQlgagravRVAadapnavahvSCGQVARSQWAVIVDPdTGAELPDGEVGEIWLHGDNIGRGYWGRPEETERTF 454

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  834 -------IPDPFSAD---PAARIYKTGDLARWLpDGNIDYLGRNDFQIKVRGFR-----IEAGEIE-SRLLR-------- 889
Cdd:PRK12476  455 gaklqsrLAEGSHADgaaDDGTWLRTGDLGVYL-DGELYITGRIADLIVIDGRNhypqdIEATVAEaSPMVRrgyvtaft 533

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967  890 CPGVQ-DAVVIAREDSPGDTRLVaylcARPDAELHPAALRQQLAASLADYMipsaFVTLDALPLTPNGKLDRKA 962
Cdd:PRK12476  534 VPAEDnERLVIVAERAAGTSRAD----PAPAIDAIRAAVSRRHGLAVADVR----LVPAGAIPRTTSGKLARRA 599
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
 499-898 4.62e-18

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 90.74  E-value: 4.62e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGV--QPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDA-APVALL 575
Cdd:cd05927     6 ISYKEVAERADNIGSALRSLGGkpAPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAeISIVFC 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  576 TQSAQVAQLNstlptVLLDTPAAAACPDTNPvvqglHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTL------ 649
Cdd:cd05927    86 DAGVKVYSLE-----EFEKLGKKNKVPPPPP-----KPEDLATICYTSGTTGNPKGVMLTHGNIVSNVAGVFKIleilnk 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  650 ----------LALDHP-SRIALNASIVFDASVKNW----------IQLLSGHTLVLVP-------DALRADAHQ---LWR 698
Cdd:cd05927   156 inptdvyisyLPLAHIfERVVEALFLYHGAKIGFYsgdirlllddIKALKPTVFPGVPrvlnriyDKIFNKVQAkgpLKR 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  699 YFARHAVDLfdctpvQLQWLLDAGLGSDP-----AYQPAQVLIGGE---------AISPAVWSRLQSLSDTRFINVYGPT 764
Cdd:cd05927   236 KLFNFALNY------KLAELRSGVVRASPfwdklVFNKIKQALGGNvrlmltgsaPLSPEVLEFLRVALGCPVLEGYGQT 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  765 ECTVDATACVVDRTQPlPTIGKPLANT--RL-------YilDAQDqPVPigvTGELHIGGAGVARGYLHRPDLTAERFIP 835
Cdd:cd05927   310 ECTAGATLTLPGDTSV-GHVGGPLPCAevKLvdvpemnY--DAKD-PNP---RGEVCIRGPNVFSGYYKDPEKTAEALDE 382

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 641744967  836 DPFsadpaariYKTGDLARWLPDGNIDYLGR--NDFQIkVRGFRIEAGEIESRLLRCPGVQDAVV 898
Cdd:cd05927   383 DGW--------LHTGDIGEWLPNGTLKIIDRkkNIFKL-SQGEYVAPEKIENIYARSPFVAQIFV 438
PRK07769 PRK07769
long-chain-fatty-acid--CoA ligase; Validated
 2628-3000 5.72e-18

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 181109 [Multi-domain]  Cd Length: 631  Bit Score: 90.94  E-value: 5.72e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2628 GEA-SLSYDELNRRANRLAHHLISFGvRPDERVAICVERGLDMVVGLLGILKAGGAYVPL-DPTYP--VERLRYMLDDAK 2703
Cdd:PRK07769   51 GVArDLTWSQFGARNRAVGARLQQVT-KPGDRVAILAPQNLDYLIAFFGALYAGRIAVPLfDPAEPghVGRLHAVLDDCT 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2704 PVA-LISQSAHLGIMN--GSLP------VILLDD-----GET-RPFDNEPDTpldarkqgltprhLAYVIYTSGSTGKPK 2768
Cdd:PRK07769  130 PSAiLTTTDSAEGVRKffRARPakerprVIAVDAvpdevGATwVPPEANEDT-------------IAYLQYTSGSTRIPA 196

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2769 GVMVEH----ANMVNFLCSMRKEPGIAqedvllGVTSLSF--DISILEIFLPLLNGarlilatqaqaadaqqlamlierH 2842
Cdd:PRK07769  197 GVQITHlnlpTNVLQVIDALEGQEGDR------GVSWLPFfhDMGLITVLLPALLG-----------------------H 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2843 AVSFMqaTPSTW-----RMLVEL--------RDFALPPGFK-ALCGGEALP-------------------ENLATALLQK 2889
Cdd:PRK07769  248 YITFM--SPAAFvrrpgRWIRELarkpggtgGTFSAAPNFAfEHAAARGLPkdgeppldlsnvkgllngsEPVSPASMRK 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2890 VTTLWNLYGPTETTI-------WSTLNGLTTPT---PYIGH------------------PIANTQI-----------YIL 2930
Cdd:PRK07769  326 FNEAFAPYGLPPTAIkpsygmaEATLFVSTTPMdeePTVIYvdrdelnagrfvevpadaPNAVAQVsagkvgvsewaVIV 405

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2931 DA-QGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERF---ITDPFS-----GAPE-ARMYKTGDLGRWLpDGTLEYLGR 3000
Cdd:PRK07769  406 DPeTASELPDGQIGEIWLHGNNIGTGYWGKPEETAATFqniLKSRLSeshaeGAPDdALWVRTGDYGVYF-DGELYITGR 484
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 616-963 6.04e-18

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 88.69  E-value: 6.04e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  616 LAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRI--ALNASIVFDASVKNWIQLLSG-HTLVLVPDALRAD 692
Cdd:cd05944     4 VAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLlcGLPLFHVNGSVVTLLTPLASGaHVVLAGPAGYRNP 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  693 A--HQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAyQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVDA 770
Cdd:cd05944    84 GlfDNFWKLVERYRITSLSTVPTVYAALLQVPVNADIS-SLRFAMSGAAPLPVELRARFEDATGLPVVEGYGLTEATCLV 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  771 TACVVDRTQPLPTIGKPL--ANTRLYILDAQDQPV-PIGV--TGELHIGGAGVARGYLHRPDltaerfipdPFSADPAAR 845
Cdd:cd05944   163 AVNPPDGPKRPGSVGLRLpyARVRIKVLDGVGRLLrDCAPdeVGEICVAGPGVFGGYLYTEG---------NKNAFVADG 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  846 IYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPA 925
Cdd:cd05944   234 WLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVVEEE 313

                         330       340       350
                  ....*....|....*....|....*....|....*....
gi 641744967  926 ALRQQLAASLADY-MIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05944   314 ELLAWARDHVPERaAVPKHIEVLEELPVTAVGKVFKPAL 352
PRK05857 PRK05857
fatty acid--CoA ligase;
1522-2033 6.05e-18

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 90.45  E-value: 6.05e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1522 ADFPhdALIHQLVEDQAARTPDTTAVLFED--QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAIL 1599
Cdd:PRK05857   10 PQLP--STVLDRVFEQARQQPEAIALRRCDgtSALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1600 KAGAAYVPLDPGYPAERLAYMLDDASPVALLTQ------ANQRALLTGDVPRILLDTADFSHLSEDNPHV------PGLD 1667
Cdd:PRK05857   88 KLGAIAVMADGNLPIAAIERFCQITDPAAALVApgskmaSSAVPEALHSIPVIAVDIAAVTRESEHSLDAaslagnADQG 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1668 AHHLAYVIYTSGSTGKPKGVMNSHRAL-----------CNRLVWMQNTYRLTPddrvlqkTPFSFDVSVWEFFWPLLYGA 1736
Cdd:PRK05857  168 SEDPLAMIFTSGTTGEPKAVLLANRTFfavpdilqkegLNWVTWVVGETTYSP-------LPATHIGGLWWILTCLMHGG 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1737 RLVMarpdGHKDAAYLAQLIERTGITTLHFVPSMLQQFV-QWADADCACDSLRRVICSG-EALPAELqqRFFARFNAQLH 1814
Cdd:PRK05857  241 LCVT----GGENTTSLLEILTTNAVATTCLVPTLLSKLVsELKSANATVPSLRLVGYGGsRAIAADV--RFIEATGVRTA 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1815 NLYGPTEAAIDVTfwaCQPDDHRSFVPI-----GRPIANTQLYILDTLG-QPVPLGVA-----GELHIGGVGVARGYLNR 1883
Cdd:PRK05857  315 QVYGLSETGCTAL---CLPTDDGSIVKIeagavGRPYPGVDVYLAATDGiGPTAPGAGpsasfGTLWIKSPANMLGYWNN 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1884 PDLTAERFIpDPFINqpgarlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDsP 1963
Cdd:PRK05857  392 PERTAEVLI-DGWVN--------TGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPD-E 461

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1964 GDTRLVAyLCPQPGVTPDPA---DLRQQLGQHL---AEYMV-PGAFVTLDAFPLTPNGKLDRKALPAP---DQSAVATRD 2033
Cdd:PRK05857  462 EFGALVG-LAVVASAELDESaarALKHTIAARFrreSEPMArPSTIVIVTDIPRTQSGKVMRASLAAAataDKARVVVRG 540
AMP-binding_C pfam13193
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ...
 1940-2015 7.54e-18

AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.


Pssm-ID: 463804 [Multi-domain]  Cd Length: 76  Bit Score: 80.28  E-value: 7.54e-18
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967  1940 EIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGK 2015
Cdd:pfam13193    1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
PRK06060 PRK06060
p-hydroxybenzoic acid--AMP ligase FadD22;
2627-3099 9.63e-18

p-hydroxybenzoic acid--AMP ligase FadD22;


Pssm-ID: 180374 [Multi-domain]  Cd Length: 705  Bit Score: 90.48  E-value: 9.63e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2627 FGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTypVERLRYMLDDAkpva 2706
Cdd:PRK06060   26 YAADVVTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPE--LHRDDHALAAR---- 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2707 liSQSAHLGIMNGSL-----------PVILLDDG-ETRPFDNEPdtpldarkqgLTPRHLAYVIYTSGSTGKPKGVMVEH 2774
Cdd:PRK06060  100 --NTEPALVVTSDALrdrfqpsrvaeAAELMSEAaRVAPGGYEP----------MGGDALAYATYTSGTTGPPKAAIHRH 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2775 ANMVNFLCSM-RKEPGIAQEDVLLGVTSLSFDISI-LEIFLPLLNGARLILatQAQAADAQQLAMLIERHAVSFMQATPS 2852
Cdd:PRK06060  168 ADPLTFVDAMcRKALRLTPEDTGLCSARMYFAYGLgNSVWFPLATGGSAVI--NSAPVTPEAAAILSARFGPSVLYGVPN 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2853 TWRMLVelrDFALPPGFKAL-C---GGEALPENLATALLQKVTTLWNLYGPTETTIWSTL--NGLTTPTP-YIGHPIANT 2925
Cdd:PRK06060  246 FFARVI---DSCSPDSFRSLrCvvsAGEALELGLAERLMEFFGGIPILDGIGSTEVGQTFvsNRVDEWRLgTLGRVLPPY 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2926 QIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPD--LTAERFItdpfsgapearmyKTGDLGRWLPDGTLEYLGRNDF 3003
Cdd:PRK06060  323 EIRVVAPDGTTAGPGVEGDLWVRGPAIAKGYWNRPDspVANEGWL-------------DTRDRVCIDSDGWVTYRCRADD 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3004 QVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPA---DLRQRLSEGVAEYMIPSAFVT 3080
Cdd:PRK06060  390 TEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSvmrDLHRGLLNRLSAFKVPHRFAV 469

                         490
                  ....*....|....*....
gi 641744967 3081 LDAFPLTPNGKLDRKALPA 3099
Cdd:PRK06060  470 VDRLPRTPNGKLVRGALRK 488
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
 2614-3092 1.05e-17

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 89.68  E-value: 1.05e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2614 AQVActPDAIAVVFGEA--SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYP 2691
Cdd:PRK13390    7 AQIA--PDRPAVIVAETgeQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLT 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2692 VERLRYMLDDAKPVALISQSAHLGIM---NGSLPVILLDDGETRPFDNEPDTpLDARKQGLTPRHL-AYVIYTSGSTGKP 2767
Cdd:PRK13390   85 APEADYIVGDSGARVLVASAALDGLAakvGADLPLRLSFGGEIDGFGSFEAA-LAGAGPRLTEQPCgAVMLYSSGTTGFP 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2768 KGVmveHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIF--LPL-------LNGARLILATQAQAADAQQLaml 2838
Cdd:PRK13390  164 KGI---QPDLPGRDVDAPGDPIVAIARAFYDISESDIYYSSAPIYhaAPLrwcsmvhALGGTVVLAKRFDAQATLGH--- 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2839 IERHAVSFMQATPSTWRMLVELRD-----FALPPGFKALCGGEALPENLATALLQKV-TTLWNLYGPTET---TIWSTLN 2909
Cdd:PRK13390  238 VERYRITVTQMVPTMFVRLLKLDAdvrtrYDVSSLRAVIHAAAPCPVDVKHAMIDWLgPIVYEYYSSTEAhgmTFIDSPD 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2910 GLTTPTPyIGHPIANTqIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAErfitdpfSGAPEARMYKT-GDLGR 2988
Cdd:PRK13390  318 WLAHPGS-VGRSVLGD-LHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAA-------AQHPAHPFWTTvGDLGS 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2989 WLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSP-GDK-RLVAYLLAQPDTVLEPA-DLRQRL 3065
Cdd:PRK13390  389 VDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEmGEQvKAVIQLVEGIRGSDELArELIDYT 468

                         490       500
                  ....*....|....*....|....*..
gi 641744967 3066 SEGVAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:PRK13390  469 RSRIAHYKAPRSVEFVDELPRTPTGKL 495
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
 2757-3092 1.40e-17

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 86.79  E-value: 1.40e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2757 VIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVT----SLSFDISILeifLPLLNGARLIlatQAQAADA 2832
Cdd:cd17638     5 IMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYLIINpffhTFGYKAGIV---ACLLTGATVV---PVAVFDV 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2833 QQLAMLIERHAVSFMQATPSTWRMLV---ELRDFALPPGFKALCGGEALPENLATALLQKVT--TLWNLYGPTET---TI 2904
Cdd:cd17638    79 DAILEAIERERITVLPGPPTLFQSLLdhpGRKKFDLSSLRAAVTGAATVPVELVRRMRSELGfeTVLTAYGLTEAgvaTM 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2905 WSTLNGLTTPTPYIGHPIANTQIYILDaqgrvvplgvAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTG 2984
Cdd:cd17638   159 CRPGDDAETVATTCGRACPGFEVRIAD----------DGEVLVRGYNVMQGYLDDPEATAEAIDADGW--------LHTG 220

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2985 DLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQR 3064
Cdd:cd17638   221 DVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGVTLTEEDVIAW 300

                         330       340
                  ....*....|....*....|....*...
gi 641744967 3065 LSEGVAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:cd17638   301 CRERLANYKVPRFVRFLDELPRNASGKV 328
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
 439-963 1.47e-17

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 89.27  E-value: 1.47e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  439 TPQQPALSQPILPKSERQQVLVDFNATDADFpremlIQQRFEAQAEQTPEAIAVlfEDQHLTYRELNRRANQLAHHLIAL 518
Cdd:PLN02330    3 MEIQKQEDNEHIFRSRYPSVPVPDKLTLPDF-----VLQDAELYADKVAFVEAV--TGKAVTYGEVVRDTRRFAKALRSL 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  519 GVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQLNST-LPTVLLDTP- 596
Cdd:PLN02330   76 GLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTALESEIKKQAEAAGAKLIVTNDTNYGKVKGLgLPVIVLGEEk 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  597 ------------AAAACPDTNpVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVI-NLATGLH----------TLLALD 653
Cdd:PLN02330  156 iegavnwkelleAADRAGDTS-DNEEILQTDLCALPFSSGTTGISKGVMLTHRNLVaNLCSSLFsvgpemigqvVTLGLI 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  654 HPSRIALNASIVFdASVKNwiqllSGHTLVLVPDALRADAHQLWRYFARHA---------------VDLFDCTPVQLQWL 718
Cdd:PLN02330  235 PFFHIYGITGICC-ATLRN-----KGKVVVMSRFELRTFLNALITQEVSFApivppiilnlvknpiVEEFDLSKLKLQAI 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  719 LDAGLGSDPayqpaQVLIGGEAISPAVwsRLQSlsdtrfinVYGPTECT-VDATACVVDRTQPLP---TIGKPLANTRLY 794
Cdd:PLN02330  309 MTAAAPLAP-----ELLTAFEAKFPGV--QVQE--------AYGLTEHScITLTHGDPEKGHGIAkknSVGFILPNLEVK 373

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  795 ILDAQD-QPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKV 873
Cdd:PLN02330  374 FIDPDTgRSLPKNTPGELCVRSQCVMQGYYNNKEETDRTIDEDGW--------LHTGDIGYIDDDGDIFIVDRIKELIKY 445

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  874 RGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLT 953
Cdd:PLN02330  446 KGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACVVINPKAKESEEDILNFVAANVAHYKKVRVVQFVDSIPKS 525

                         570
                  ....*....|
gi 641744967  954 PNGKLDRKAL 963
Cdd:PLN02330  526 LSGKIMRRLL 535
AFD_CAR-like cd17632
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ...
 499-958 1.67e-17

adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.


Pssm-ID: 341287 [Multi-domain]  Cd Length: 588  Bit Score: 89.44  E-value: 1.67e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAH-HLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPvALLTQ 577
Cdd:cd17632    68 ITYAELWERVGAVAAaHDPEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEP-RLLAV 146

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  578 S------AQVAQLNSTLP-------------------------------TVLLDTPAAAACPDTNP---VVQGLHAAHLA 617
Cdd:cd17632   147 SaehldlAVEAVLEGGTPprlvvfdhrpevdahraalesarerlaavgiPVTTLTLIAVRGRDLPPaplFRPEPDDDPLA 226

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  618 YVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWI--QLLSGHTLVLVPdalRADAHQ 695
Cdd:cd17632   227 LLIYTSGSTGTPKGAMYTERLVATFWLKVSSIQDIRPPASITLNFMPMSHIAGRISLygTLARGGTAYFAA---ASDMST 303

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  696 LWRYFA-----------RHAVDLFDCTPVQLQWLLDAglGSDPAYQPAQV----------------LIGGEAISPAVWSR 748
Cdd:cd17632   304 LFDDLAlvrptelflvpRVCDMLFQRYQAELDRRSVA--GADAETLAERVkaelrervlggrllaaVCGSAPLSAEMKAF 381

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  749 LQSLSDTRFINVYGPTEctvdATACVVD-RTQPLPTIGKPLANTRLYILDAQDQPVPigvTGELHIGGAGVARGYLHRPD 827
Cdd:cd17632   382 MESLLDLDLHDGYGSTE----AGAVILDgVIVRPPVLDYKLVDVPELGYFRTDRPHP---RGELLVKTDTLFPGYYKRPE 454

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  828 LTAERFIPDPFsadpaariYKTGD-LARWLPDgNIDYLGRNDFQIK------VRGFRIEAGEIESRLLRCPGVQD----- 895
Cdd:cd17632   455 VTAEVFDEDGF--------YRTGDvMAELGPD-RLVYVDRRNNVLKlsqgefVTVARLEAVFAASPLVRQIFVYGnsera 525

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967  896 ---AVVIaredsPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVtLDALPLTP-NGKL 958
Cdd:cd17632   526 yllAVVV-----PTQDALAGEDTARLRAALAESLQRIAREAGLQSYEIPRDFL-IETEPFTIaNGLL 586
PLN02479 PLN02479
acetate-CoA ligase
 1676-2023 1.70e-17

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 89.13  E-value: 1.70e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1676 YTSGSTGKPKGVMNSHR-----ALCNRLVWMqntyrlTPDDRVLQKTPFSFDVSVWEFFWPL--LYGARLVMArpdgHKD 1748
Cdd:PLN02479  202 YTSGTTASPKGVVLHHRgaylmALSNALIWG------MNEGAVYLWTLPMFHCNGWCFTWTLaaLCGTNICLR----QVT 271

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1749 AAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLR--RVICSGEALPAEL-----QQRFFARFNAQLHNLYGPTE 1821
Cdd:PLN02479  272 AKAIYSAIANYGVTHFCAAPVVLNTIVNAPKSETILPLPRvvHVMTAGAAPPPSVlfamsEKGFRVTHTYGLSETYGPST 351

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1822 AAIDVTFWACQPDDHRSFVPIG---RPIANTQLYILDT-LGQPVPL--GVAGELHIGGVGVARGYLNRPDLTAERFipdp 1895
Cdd:PLN02479  352 VCAWKPEWDSLPPEEQARLNARqgvRYIGLEGLDVVDTkTMKPVPAdgKTMGEIVMRGNMVMKGYLKNPKANEEAF---- 427

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1896 finQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQ 1975
Cdd:PLN02479  428 ---ANG--WFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLK 502

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|...
gi 641744967 1976 PGV-TPDPADLRQQLGQ----HLAEYMVPGAfVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PLN02479  503 PGVdKSDEAALAEDIMKfcreRLPAYWVPKS-VVFGPLPKTATGKIQKHVLRA 554
FCS cd05921
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ...
 480-936 2.41e-17

Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.


Pssm-ID: 341245 [Multi-domain]  Cd Length: 561  Bit Score: 88.64  E-value: 2.41e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  480 EAQAEQTPEAIAVLFED-----QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMD 554
Cdd:cd05921     2 AHWARQAPDRTWLAEREgnggwRRVTYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVS 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  555 PAYPA-----ERLAYILD----------DAAP------------VALLTQSAQVAQLNSTLPTVLLDTPAAAACPDTNPV 607
Cdd:cd05921    82 PAYSLmsqdlAKLKHLFEllkpglvfaqDAAPfaralaaifplgTPLVVSRNAVAGRGAISFAELAATPPTAAVDAAFAA 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  608 VQglhAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLAL--DHPSRIA--LNASIVFDASVKNWIQLLSGHTLV 683
Cdd:cd05921   162 VG---PDTVAKFLFTSGSTGLPKAVINTQRMLCANQAMLEQTYPFfgEEPPVLVdwLPWNHTFGGNHNFNLVLYNGGTLY 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  684 LvpDALRADAHQlwryFARHAVDLFDCTP-----VQLQW-LLDAGLGSDPA-----YQPAQVLI-GGEAISPAVWSRLQS 751
Cdd:cd05921   239 I--DDGKPMPGG----FEETLRNLREISPtvyfnVPAGWeMLVAALEKDEAlrrrfFKRLKLMFyAGAGLSQDVWDRLQA 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  752 LSDT------RFINVYGPTECTVDATACVVDRTQPlPTIGKPLANTRLYIldaqdqpVPIGVTGELHIGGAGVARGYLHR 825
Cdd:cd05921   313 LAVAtvgeriPMMAGLGATETAPTATFTHWPTERS-GLIGLPAPGTELKL-------VPSGGKYEVRVKGPNVTPGYWRQ 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  826 PDLTAERFIPDPFsadpaariYKTGDLARWL----PDGNIDYLGR--NDFQIKvRGFRIEAGEIESRLLRC--PGVQDAV 897
Cdd:cd05921   385 PELTAQAFDEEGF--------YCLGDAAKLAdpddPAKGLVFDGRvaEDFKLA-SGTWVSVGPLRARAVAAcaPLVHDAV 455

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967  898 VIA--RE--------DSPGDTRLVAYLCARPDAELHPAALRQQLAASLA 936
Cdd:cd05921   456 VAGedRAevgalvfpDLLACRRLVGLQEASDAEVLRHAKVRAAFRDRLA 504
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
 2602-3092 2.56e-17

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 88.68  E-value: 2.56e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2602 IPRHALIHelfeaqvactPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGG 2681
Cdd:PRK07786   23 LARHALMQ----------PDAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGA 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2682 AYVPLDPTYPVERLRYMLDDAKPVALISQSAHLGIMNG------SLPVILLDDGETRPF----------DNEPDTPLDAR 2745
Cdd:PRK07786   93 IAVPVNFRLTPPEIAFLVSDCGAHVVVTEAALAPVATAvrdivpLLSTVVVAGGSSDDSvlgyedllaeAGPAHAPVDIP 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2746 KQglTPrhlAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLP-LLNGARLILA 2824
Cdd:PRK07786  173 ND--SP---ALIMYTSGTTGRPKGAVLTHANLTGQAMTCLRTNGADINSDVGFVGVPLFHIAGIGSMLPgLLLGAPTVIY 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2825 TQAQAADAQQLAMLiERHAVSFMQATPSTWRML-----VELRDFALppgfKALCGGEA-LPENLATALLQKVTTLWNL-- 2896
Cdd:PRK07786  248 PLGAFDPGQLLDVL-EAEKVTGIFLVPAQWQAVcaeqqARPRDLAL----RVLSWGAApASDTLLRQMAATFPEAQILaa 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2897 YGPTETT-IWSTLNG--LTTPTPYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFitdpfs 2973
Cdd:PRK07786  323 FGQTEMSpVTCMLLGedAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAF------ 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2974 gapEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYL-LAQP 3052
Cdd:PRK07786  397 ---AGGWFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAaVRND 473

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967 3053 DTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:PRK07786  474 DAALTLEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKV 513
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
 2612-2785 2.63e-17

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 88.78  E-value: 2.63e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2612 FEAQVACTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYP 2691
Cdd:PRK08279   43 FEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQR 122

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2692 VERLRYMLDDAKPVALI----------SQSAHLGIMngslPVILLDDGETRPF-----------DNEPDTPLDARkQGLT 2750
Cdd:PRK08279  123 GAVLAHSLNLVDAKHLIvgeelveafeEARADLARP----PRLWVAGGDTLDDpegyedlaaaaAGAPTTNPASR-SGVT 197

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 641744967 2751 PRHLAYVIYTSGSTGKPKGVMVEHAnmvNFLCSMR 2785
Cdd:PRK08279  198 AKDTAFYIYTSGTTGLPKAAVMSHM---RWLKAMG 229
AcpA COG3433
Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites ...
 2899-3187 3.27e-17

Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442659 [Multi-domain]  Cd Length: 295  Bit Score: 85.19  E-value: 3.27e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2899 PTETTIWSTLNGLTTPTPYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFSGAPea 2978
Cdd:COG3433     1 IAIATPPPAPPTPDEPPPVIPPAIVQARALLLIVDLQGYFGGFGGEGGLLGAGLLLRIRLLAAAARAPFIPVPYPAQP-- 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2979 rmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIARED--SPGDKRLVAYLLAQPDTVL 3056
Cdd:COG3433    79 --GRQADDLRLLLRRGLGPGGGLERLVQQVVIRAERGEEEELLLVLRAAAVVRVAVLAAlrGAGVGLLLIVGAVAALDGL 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3057 EPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPAPDQSAMATRGYEAP---QGDLEHALAQIWQTLLGV-- 3131
Cdd:COG3433   157 AAAAALAALDKVPPDVVAASAVVALDALLLLALKVVARAAPALAAAEALLAAASPAPaleTALTEEELRADVAELLGVdp 236

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 3132 ERVGRHDHFFELGGHSLLAVQLNARIRAEFLtDIPIVAIFQHPQLSALAEVILAAQ 3187
Cdd:COG3433   237 EEIDPDDNLFDLGLDSIRLMQLVERWRKAGL-DVSFADLAEHPTLAAWWALLAAAQ 291
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
 2757-3097 3.91e-17

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 87.91  E-value: 3.91e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2757 VIYTSGSTGKPKgvMVEHAN---MVNFLCSMRKEPGIAQEDVLLGVTSLSFDISIL-EIFLPLLNGARLILATQAQAADA 2832
Cdd:cd05928   179 IYFTSGTTGSPK--MAEHSHsslGLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWsSLFEPWIQGACVFVHHLPRFDPL 256

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2833 QQLAMLIERHAVSFMQAtPSTWRMLVE--LRDFALPPGFKALCGGEAL-PENLATALLQKVTTLWNLYGPTETT-IWSTL 2908
Cdd:cd05928   257 VILKTLSSYPITTFCGA-PTVYRMLVQqdLSSYKFPSLQHCVTGGEPLnPEVLEKWKAQTGLDIYEGYGQTETGlICANF 335

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 NGLTTPTPYIGHPIANTQIYILDAQGRVVPLGVAGEIHI-----AGAGVVRGYLGRPDLTAERFITDpfsgapearMYKT 2983
Cdd:cd05928   336 KGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIrvkpiRPFGLFSGYVDNPEKTAATIRGD---------FYLT 406

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2984 GDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYL-LAQPDTVLEPADLR 3062
Cdd:cd05928   407 GDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAFVvLAPQFLSHDPEQLT 486

                         330       340       350
                  ....*....|....*....|....*....|....*....
gi 641744967 3063 QRLSEGV----AEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05928   487 KELQQHVksvtAPYKYPRKVEFVQELPKTVTGKIQRNEL 525
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
 487-960 4.43e-17

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 87.93  E-value: 4.43e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYIL 566
Cdd:PLN02860   21 GNAVVTISGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGIVAPLNYRWSFEEAKSAM 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  567 DDAAPVALLT----QSAQVAQLNSTLPT----VLLDTPAAAACPDTNPVV-------QGLHAAHLAY---------VIYT 622
Cdd:PLN02860  101 LLVRPVMLVTdetcSSWYEELQNDRLPSlmwqVFLESPSSSVFIFLNSFLttemlkqRALGTTELDYawapddavlICFT 180

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  623 SGSTGRPKGVMVAHRNVI--NLA----TG-------LHTlLALDH----PSRIAL-------------NASIVFDASVKN 672
Cdd:PLN02860  181 SGTTGRPKGVTISHSALIvqSLAkiaiVGygeddvyLHT-APLCHigglSSALAMlmvgachvllpkfDAKAALQAIKQH 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  673 WIQllsghTLVLVPdALRADahqLWRYFARHAVDlfDCTPvQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRLQSL 752
Cdd:PLN02860  260 NVT-----SMITVP-AMMAD---LISLTRKSMTW--KVFP-SVRKILNGGGSLSSRLLPDAKKLFPNAKLFSAYGMTEAC 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  753 SDTRFINVYGPTECTVDATACVVDRTQPLPT-------IGKPLANTRLYIldAQDQPVPigvTGELHIGGAGVARGYLHR 825
Cdd:PLN02860  328 SSLTFMTLHDPTLESPKQTLQTVNQTKSSSVhqpqgvcVGKPAPHVELKI--GLDESSR---VGRILTRGPHVMLGYWGQ 402

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  826 PDLTAERFIPDPFSAdpaariykTGDLArWLPD-GNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDS 904
Cdd:PLN02860  403 NSETASVLSNDGWLD--------TGDIG-WIDKaGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDS 473

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967  905 PGDTRLVAylCAR-------PDAELHPAALRQQLAAS----------LADYMIPSAFVTL-DALPLTPNGKLDR 960
Cdd:PLN02860  474 RLTEMVVA--CVRlrdgwiwSDNEKENAKKNLTLSSEtlrhhcreknLSRFKIPKLFVQWrKPFPLTTTGKIRR 545
PRK08308 PRK08308
acyl-CoA synthetase; Validated
 1551-2023 4.77e-17

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 86.63  E-value: 4.77e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1551 DQHLTYDALNRRANQLAHHLIdLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALL 1630
Cdd:PRK08308    6 DEEYSKSDFDLRLQRYEEMEQ-FQEAAGNRFAVCLKDPFDIITLVFFLKEKGASVLPIHPDTPKEAAIRMAKRAGCHGLL 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1631 TQanqrallTGDVPRILLDTADfshlsednPHVPGLdahhlayVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPD 1710
Cdd:PRK08308   85 YG-------ESDFTKLEAVNYL--------AEEPSL-------LQYSSGTTGEPKLIRRSWTEIDREIEAYNEALNCEQD 142

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1711 DRVLQKTPFSFDvsvweffWPLLYGARLVMARPD-----GHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADcacD 1785
Cdd:PRK08308  143 ETPIVACPVTHS-------YGLICGVLAALTRGSkpviiTNKNPKFALNILRNTPQHILYAVPLMLHILGRLLPGT---F 212

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1786 SLRRVICSGEALPAELQQRFFARFNaQLHNLYGPTEAAidvtfwaCqpddhrsfVPIgrpiaNTQLYILDTLGQPVPlgv 1865
Cdd:PRK08308  213 QFHAVMTSGTPLPEAWFYKLRERTT-YMMQQYGCSEAG-------C--------VSI-----CPDMKSHLDLGNPLP--- 268

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1866 agelHiggVGVARGylnrpdltAERFIPDPFINQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARL 1945
Cdd:PRK08308  269 ----H---VSVSAG--------SDENAPEEIVVKMGDKEIFTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVM 333

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 1946 MQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVtpDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK08308  334 LRLPGVQEAVVYRGKDPVAGERVKAKVISHEEI--DPVQLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLLEL 409
PLN02574 PLN02574
4-coumarate--CoA ligase-like
 2631-3097 4.84e-17

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 87.59  E-value: 4.84e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLI-SFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALIS 2709
Cdd:PLN02574   66 SISYSELQPLVKSMAAGLYhVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFT 145

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2710 QSAHLGIMNG-SLPVILLDDG---ETRPFDNEPDTPLDARKQGLTPRHL------AYVIYTSGSTGKPKGVMVEHANM-- 2777
Cdd:PLN02574  146 SPENVEKLSPlGVPVIGVPENydfDSKRIEFPKFYELIKEDFDFVPKPVikqddvAAIMYSSGTTGASKGVVLTHRNLia 225

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2778 -----VNFLCSMRKEPGiaQEDVLLGVTSLsFDISILEIF-LPLLNGARLILATQAQAADAQQLamLIERHAVSFMQATP 2851
Cdd:PLN02574  226 mvelfVRFEASQYEYPG--SDNVYLAALPM-FHIYGLSLFvVGLLSLGSTIVVMRRFDASDMVK--VIDRFKVTHFPVVP 300

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2852 StwrMLVELRDFALPPG---FKAL----CGGEALPENLATALLQKV--TTLWNLYGPTETTIWSTlNGLTTPT----PYI 2918
Cdd:PLN02574  301 P---ILMALTKKAKGVCgevLKSLkqvsCGAAPLSGKFIQDFVQTLphVDFIQGYGMTESTAVGT-RGFNTEKlskySSV 376

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2919 GHPIANTQIYILD-AQGRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEY 2997
Cdd:PLN02574  377 GLLAPNMQAKVVDwSTGCLLPPGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGW--------LRTGDIAYFDEDGYLYI 448

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2998 LGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSA 3077
Cdd:PLN02574  449 VDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQEAVINYVAKQVAPYKKVRK 528

                         490       500
                  ....*....|....*....|
gi 641744967 3078 FVTLDAFPLTPNGKLDRKAL 3097
Cdd:PLN02574  529 VVFVQSIPKSPAGKILRREL 548
PRK09192 PRK09192
fatty acyl-AMP ligase;
1554-2018 5.12e-17

fatty acyl-AMP ligase;


Pssm-ID: 236403 [Multi-domain]  Cd Length: 579  Bit Score: 87.75  E-value: 5.12e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLD-PGYPAERLAY------MLDDASP 1626
Cdd:PRK09192   50 LPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGLVPVPLPlPMGFGGRESYiaqlrgMLASAQP 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1627 VALLTQANQRALL----TGDVPRILLDTADFSHLSEDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM- 1701
Cdd:PRK09192  130 AAIITPDELLPWVneatHGNPLLHVLSHAWFKALPEADVALPRPTPDDIAYLQYSSGSTRFPRGVIITHRALMANLRAIs 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1702 QNTYRLTPDDRVLQKTPFSFDVSVWEFFW-PL-------LYGARLVMARPdghkdAAYLaQLIERTGiTTLHFVPS---- 1769
Cdd:PRK09192  210 HDGLKVRPGDRCVSWLPFYHDMGLVGFLLtPVatqlsvdYLPTRDFARRP-----LQWL-DLISRNR-GTISYSPPfgye 282

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1770 MLQQFVQWAD-ADCACDSLRRVICSGEALPAELQQRFFARFnAQLH-------NLYGPTEAAIDVTF------------- 1828
Cdd:PRK09192  283 LCARRVNSKDlAELDLSCWRVAGIGADMIRPDVLHQFAEAF-APAGfddkafmPSYGLAEATLAVSFsplgsgivveevd 361

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1829 ------------WACQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDlTAERFIPDPF 1896
Cdd:PRK09192  362 rdrleyqgkavaPGAETRRVRTFVNCGKALPGHEIEIRNEAGMPLPERVVGHICVRGPSLMSGYFRDEE-SQDVLAADGW 440

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1897 INqpgarlykTGDLArWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQ--EAVVVAREDSPGDTRLVAYLCP 1974
Cdd:PRK09192  441 LD--------TGDLG-YLLDGYLYITGRAKDLIIINGRNIWPQDIEWIAEQEPELRsgDAAAFSIAQENGEKIVLLVQCR 511

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 641744967 1975 qpgvTPDPADlRQQLGQHLAE--YMVPGAFVTLD-----AFPLTPNGKLDR 2018
Cdd:PRK09192  512 ----ISDEER-RGQLIHALAAlvRSEFGVEAAVElvpphSLPRTSSGKLSR 557
PRK08162 PRK08162
acyl-CoA synthetase; Validated
 1542-2021 5.50e-17

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 87.31  E-value: 5.50e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1542 PDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML 1621
Cdd:PRK08162   32 PDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFML 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1622 DDASPVALLT-----QANQRALLTGDVPRIL-----------------LDTADFshLSEDNPHVPGL------DAHHLAy 1673
Cdd:PRK08162  112 RHGEAKVLIVdtefaEVAREALALLPGPKPLvidvddpeypggrfigaLDYEAF--LASGDPDFAWTlpadewDAIALN- 188

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1674 viYTSGSTGKPKGVMNSHR-----ALCNRLVWmqntyRLTPDDRVLQKTPFsFDVSVWEFFWPLLYGA------RLVmar 1742
Cdd:PRK08162  189 --YTSGTTGNPKGVVYHHRgaylnALSNILAW-----GMPKHPVYLWTLPM-FHCNGWCFPWTVAARAgtnvclRKV--- 257

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1743 pdghkDAAYLAQLIERTGITtlHF-----VPSMLqqfvqwADADcacDSLRRVI-------CSGEALPAELQQRfFARFN 1810
Cdd:PRK08162  258 -----DPKLIFDLIREHGVT--HYcgapiVLSAL------INAP---AEWRAGIdhpvhamVAGAAPPAAVIAK-MEEIG 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1811 AQLHNLYGPTEAAIDVTFWACQPDDHRsfVPIG-----------RPIANTQLYILDT-LGQPVPLG--VAGELHIGGVGV 1876
Cdd:PRK08162  321 FDLTHVYGLTETYGPATVCAWQPEWDA--LPLDeraqlkarqgvRYPLQEGVTVLDPdTMQPVPADgeTIGEIMFRGNIV 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1877 ARGYLNRPDLTAERFipdpfinQPGarLYKTGDLARWLPDGsleYLgrndfQVKLR--------GFRIELGEIEARLMQC 1948
Cdd:PRK08162  399 MKGYLKNPKATEEAF-------AGG--WFHTGDLAVLHPDG---YI-----KIKDRskdiiisgGENISSIEVEDVLYRH 461

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967 1949 PGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAfVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK08162  462 PAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEEEIIAHCREHLAGFKVPKA-VVFGELPKTSTGKIQKFVL 533
PRK12582 PRK12582
acyl-CoA synthetase; Provisional
 2631-3063 5.72e-17

acyl-CoA synthetase; Provisional


Pssm-ID: 237144 [Multi-domain]  Cd Length: 624  Bit Score: 87.79  E-value: 5.72e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPV-----ERLRYMLDDAKPV 2705
Cdd:PRK12582   80 KVTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAAMQAGVPAAPVSPAYSLmshdhAKLKHLFDLVKPR 159

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2706 ALISQS--------AHLGIMNGSLPVI--LLDDGETRPFDNEPDTP----LDARKQGLTPRHLAYVIYTSGSTGKPKGVM 2771
Cdd:PRK12582  160 VVFAQSgapfaralAALDLLDVTVVHVtgPGEGIASIAFADLAATPptaaVAAAIAAITPDTVAKYLFTSGSTGMPKAVI 239

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2772 VEHANMvnflCSMRKEPgiaqedvlLGVTSLSFD--ISILEIFLP--------------LLNGARLILATQAQAADAQQL 2835
Cdd:PRK12582  240 NTQRMM----CANIAMQ--------EQLRPREPDppPPVSLDWMPwnhtmggnanfnglLWGGGTLYIDDGKPLPGMFEE 307

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2836 AM--LIERHAVSFMQAtPSTWRMLVEL--RDFALPPG-FKAL----CGGEALPENLAT---ALLQKVT----TLWNLYGP 2899
Cdd:PRK12582  308 TIrnLREISPTVYGNV-PAGYAMLAEAmeKDDALRRSfFKNLrlmaYGGATLSDDLYErmqALAVRTTghriPFYTGYGA 386

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2900 TET------TIWSTLN-GLttptpyIGHPIANTQIyildaqgRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPF 2972
Cdd:PRK12582  387 TETaptttgTHWDTERvGL------IGLPLPGVEL-------KLAPVGDKYEVRVKGPNVTPGYHKDPELTAAAFDEEGF 453

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2973 sgapearmYKTGDLGRWL----PDGTLEYLGR--NDFQ------VKVRGFRIELgeietrLARCHGV-HDAVViaredsP 3039
Cdd:PRK12582  454 --------YRLGDAARFVdpddPEKGLIFDGRvaEDFKlstgtwVSVGTLRPDA------VAACSPViHDAVV------A 513

                         490       500
                  ....*....|....*....|....
gi 641744967 3040 GDKRLVAYLLAQPDtvlePADLRQ 3063
Cdd:PRK12582  514 GQDRAFIGLLAWPN----PAACRQ 533
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
 1674-2021 5.99e-17

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 87.14  E-value: 5.99e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1674 VIYTSGSTGKPKGVMNSHRAL------CNRLvWMQntyrLTPDDRVLQKTPFSFDVSVW-EFFWPLLYGArLVMARPDGH 1746
Cdd:cd05928   179 IYFTSGTTGSPKMAEHSHSSLglglkvNGRY-WLD----LTASDIMWNTSDTGWIKSAWsSLFEPWIQGA-CVFVHHLPR 252

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1747 KDAAYLAQLIERTGITTLHFVPSMLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAA-ID 1825
Cdd:cd05928   253 FDPLVILKTLSSYPITTFCGAPTVYRMLVQQDLSSYKFPSLQHCVTGGEPLNPEVLEKWKAQTGLDIYEGYGQTETGlIC 332

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1826 VTFWA--CQPDDhrsfvpIGRPIANTQLYILDTLGQPVPLGVAGELHI-----GGVGVARGYLNRPDLTAERFIPDpfin 1898
Cdd:cd05928   333 ANFKGmkIKPGS------MGKASPPYDVQIIDDNGNVLPPGTEGDIGIrvkpiRPFGLFSGYVDNPEKTAATIRGD---- 402

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1899 qpgarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAredSPGDTR---LVAYLCPQ 1975
Cdd:cd05928   403 -----FYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVS---SPDPIRgevVKAFVVLA 474

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|.
gi 641744967 1976 PGV-TPDPADLRQQLGQHL----AEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05928   475 PQFlSHDPEQLTKELQQHVksvtAPYKYPRKVEFVQELPKTVTGKIQRNEL 525
PLN03102 PLN03102
acyl-activating enzyme; Provisional
 2618-3124 7.30e-17

acyl-activating enzyme; Provisional


Pssm-ID: 215576 [Multi-domain]  Cd Length: 579  Bit Score: 87.38  E-value: 7.30e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2618 CTPDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRY 2697
Cdd:PLN03102   26 CYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAA 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2698 MLDDAKP---------VALISQSAHLGIMNGS---LPVILLD--DGETRPFDNEPDTPLDARKQGLTPRHLAYVI----- 2758
Cdd:PLN03102  106 ILRHAKPkilfvdrsfEPLAREVLHLLSSEDSnlnLPVIFIHeiDFPKRPSSEELDYECLIQRGEPTPSLVARMFriqde 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2759 -------YTSGSTGKPKGVMVEHANMvnFLCSMrkepgiaqeDVLLGVTSLSFDISI--LEIF------LPLLNGARLIL 2823
Cdd:PLN03102  186 hdpislnYTSGTTADPKGVVISHRGA--YLSTL---------SAIIGWEMGTCPVYLwtLPMFhcngwtFTWGTAARGGT 254

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2824 ATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELRDFALPPG---FKALCGGEALPenlaTALLQKVTTL----WNL 2896
Cdd:PLN03102  255 SVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRsgpVHVLTGGSPPP----AALVKKVQRLgfqvMHA 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2897 YGPTETT----------IWSTLNglttptpyighpiANTQIYILDAQGrVVPLGVA--------------------GEIH 2946
Cdd:PLN03102  331 YGLTEATgpvlfcewqdEWNRLP-------------ENQQMELKARQG-VSILGLAdvdvknketqesvprdgktmGEIV 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2947 IAGAGVVRGYLGRPDLTAERFitdpfsgapEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHG 3026
Cdd:PLN03102  397 IKGSSIMKGYLKNPKATSEAF---------KHGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPK 467

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3027 VHDAVVIAREDSPGDKRLVAYLLAQ----------PDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDR-- 3094
Cdd:PLN03102  468 VLETAVVAMPHPTWGETPCAFVVLEkgettkedrvDKLVTRERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKpk 547

                         570       580       590
                  ....*....|....*....|....*....|....*
gi 641744967 3095 -----KALPAPDQSAMATRGYEAPqgdLEHALAQI 3124
Cdd:PLN03102  548 lrdiaKGLVVEDEDNVIKKVHQRP---VEHFSSRL 579
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
 2617-3097 7.93e-17

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 86.81  E-value: 7.93e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2617 ACTPDAIAVV--FGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVER 2694
Cdd:cd17642    28 ASVPGTIAFTdaHTGVNYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERE 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2695 LRYMLDDAKP-VALISQSAHLGIMN--GSLP----VILLDDGE-------TRPFDNEPDTPLDARKQGLTP-----RHLA 2755
Cdd:cd17642   108 LDHSLNISKPtIVFCSKKGLQKVLNvqKKLKiiktIIILDSKEdykgyqcLYTFITQNLPPGFNEYDFKPPsfdrdEQVA 187

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2756 YVIYTSGSTGKPKGVMVEHANMVNFLCSMRkEP----GIAQEDVLLGVTSLSFDISILEIFLPLLNGARLILATQAQAAD 2831
Cdd:cd17642   188 LIMNSSGSTGLPKGVQLTHKNIVARFSHAR-DPifgnQIIPDTAILTVIPFHHGFGMFTTLGYLICGFRVVLMYKFEEEL 266

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2832 AQQLamlIERHAVSFMQATPSTWRMLVE---LRDFALPPGFKALCGGEALPENLATALLQ--KVTTLWNLYGPTETT--I 2904
Cdd:cd17642   267 FLRS---LQDYKVQSALLVPTLFAFFAKstlVDKYDLSNLHEIASGGAPLSKEVGEAVAKrfKLPGIRQGYGLTETTsaI 343

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2905 WSTLNGLTTPtpyighpiantqiyilDAQGRVVP--------------LGV--AGEIHIAGAGVVRGYLGRPDLTAERFI 2968
Cdd:cd17642   344 LITPEGDDKP----------------GAVGKVVPffyakvvdldtgktLGPneRGELCVKGPMIMKGYVNNPEATKALID 407

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2969 TDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYL 3048
Cdd:cd17642   408 KDGW--------LHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVV 479

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 3049 LAQP----------DTVLEPADLRQRLSEGVaeymipsafVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd17642   480 VLEAgktmtekevmDYVASQVSTAKRLRGGV---------KFVDEVPKGLTGKIDRRKI 529
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
 2631-3105 9.16e-17

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 86.87  E-value: 9.16e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQ 2710
Cdd:PRK04319   73 KYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITT 152

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2711 SAHLG-IMNGSLP----VILLDDGEtrpfDNEPDT-PLDARKQG---------LTPRHLAYVIYTSGSTGKPKGVMVEHA 2775
Cdd:PRK04319  153 PALLErKPADDLPslkhVLLVGEDV----EEGPGTlDFNALMEQasdefdiewTDREDGAILHYTSGSTGKPKGVLHVHN 228

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2776 NMVN----------------FLCSmrKEPGiaqedvllGVTSLSFDisileIFLPLLNGARLILATQAQAADAQQLamLI 2839
Cdd:PRK04319  229 AMLQhyqtgkyvldlheddvYWCT--ADPG--------WVTGTSYG-----IFAPWLNGATNVIDGGRFSPERWYR--IL 291

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2840 ERHAVSFMQATPSTWRMLV----EL-RDFALPPGFKALCGGEAL-PEnlATALLQKVTTLwnlygPTETTIWSTLNG--- 2910
Cdd:PRK04319  292 EDYKVTVWYTAPTAIRMLMgagdDLvKKYDLSSLRHILSVGEPLnPE--VVRWGMKVFGL-----PIHDNWWMTETGgim 364

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2911 -LTTPTPYI-----GHPIANTQIYILDAQGRVVPLGVAGEIHIAgAG---VVRGYLGRPDLTAERFITDpfsgapearMY 2981
Cdd:PRK04319  365 iANYPAMDIkpgsmGKPLPGIEAAIVDDQGNELPPNRMGNLAIK-KGwpsMMRGIWNNPEKYESYFAGD---------WY 434

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2982 KTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTvlEPAD- 3060
Cdd:PRK04319  435 VSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGY--EPSEe 512

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3061 --------LRQRLSEGVAeymiPSAFVTLDAFPLTPNGKLDR---KA----LPAPDQSAM 3105
Cdd:PRK04319  513 lkeeirgfVKKGLGAHAA----PREIEFKDKLPKTRSGKIMRrvlKAwelgLPEGDLSTM 568
PRK05620 PRK05620
long-chain fatty-acid--CoA ligase;
1543-2021 9.78e-17

long-chain fatty-acid--CoA ligase;


Pssm-ID: 180167 [Multi-domain]  Cd Length: 576  Bit Score: 86.76  E-value: 9.78e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1543 DTTAVLFED---QHLTYDALNRRANQLAHHLID-LGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLA 1618
Cdd:PRK05620   25 DTTVTTWGGaeqEQTTFAAIGARAAALAHALHDeLGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIV 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1619 YMLDDASP---VALLTQANQRALLTGDVPR----ILLDTADFSH-LSEDNPHV------------------PGLDAHHLA 1672
Cdd:PRK05620  105 HIINHAEDeviVADPRLAEQLGEILKECPCvravVFIGPSDADSaAAHMPEGIkvysyealldgrstvydwPELDETTAA 184

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1673 YVIYTSGSTGKPKGVMNSHRALcnrlvWMQNTYRLTPDD-RVLQKTPFSFDVSVWEFF-W--PL---LYGARLVMarPDG 1745
Cdd:PRK05620  185 AICYSTGTTGAPKGVVYSHRSL-----YLQSLSLRTTDSlAVTHGESFLCCVPIYHVLsWgvPLaafMSGTPLVF--PGP 257

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1746 HKDAAYLAQLIERTGITTLHFVPSM-LQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAI 1824
Cdd:PRK05620  258 DLSAPTLAKIIATAMPRVAHGVPTLwIQLMVHYLKNPPERMSLQEIYVGGSAVPPILIKAWEERYGVDVVHVWGMTETSP 337

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1825 DVTFwACQP--------DDHRsfVPIGRPIANTQLYILDTlGQPVPLG--VAGELHIGGVGVARGYLNRP---------- 1884
Cdd:PRK05620  338 VGTV-ARPPsgvsgearWAYR--VSQGRFPASLEYRIVND-GQVMESTdrNEGEIQVRGNWVTASYYHSPteegggaast 413

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1885 ------DLTAERFIPDPFInqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVA 1958
Cdd:PRK05620  414 frgedvEDANDRFTADGWL--------RTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIG 485

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 1959 REDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK05620  486 YPDDKWGERPLAVTVLAPGIEPTREtaeRLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDL 551
PLN02246 PLN02246
4-coumarate--CoA ligase
 2599-3099 1.16e-16

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 86.57  E-value: 1.16e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2599 DADIPRHALIHE-LFE--AQVACTPDAIAVVFGEaSLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLG 2675
Cdd:PLN02246   16 DIYIPNHLPLHDyCFErlSEFSDRPCLIDGATGR-VYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLG 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2676 ILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQSAH------LGIMNGsLPVILLDDGE---------TRPFDNEPDT 2740
Cdd:PLN02246   95 ASRRGAVTTTANPFYTPAEIAKQAKASGAKLIITQSCYvdklkgLAEDDG-VTVVTIDDPPegclhfselTQADENELPE 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2741 PldarkqGLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNflcsmrkepGIAQ-------------EDVLLGVTSLsFDI- 2806
Cdd:PLN02246  174 V------EISPDDVVALPYSSGTTGLPKGVMLTHKGLVT---------SVAQqvdgenpnlyfhsDDVILCVLPM-FHIy 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2807 ---SILeiFLPLLNGARLILATQAQAADAQQlamLIERHAVSFMQATP-----------------STWRMLvelrdfalp 2866
Cdd:PLN02246  238 slnSVL--LCGLRVGAAILIMPKFEIGALLE---LIQRHKVTIAPFVPpivlaiakspvvekydlSSIRMV--------- 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2867 pgfkaLCGGEALPENLATALLQKV--TTLWNLYGPTE--TTIWSTLNGLTTPTPY----IGHPIANTQIYILDAQ-GRVV 2937
Cdd:PLN02246  304 -----LSGAAPLGKELEDAFRAKLpnAVLGQGYGMTEagPVLAMCLAFAKEPFPVksgsCGTVVRNAELKIVDPEtGASL 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2938 PLGVAGEIHIAGAGVVRGYLGRPDLTAErfitdpfsgapearmykTGDLGRWLPDGTLEYLGRND--FQV-------KVR 3008
Cdd:PLN02246  379 PRNQPGEICIRGPQIMKGYLNDPEATAN-----------------TIDKDGWLHTGDIGYIDDDDelFIVdrlkeliKYK 441

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3009 GFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEY-MIPSAFVTlDAFPLT 3087
Cdd:PLN02246  442 GFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITEDEIKQFVAKQVVFYkRIHKVFFV-DSIPKA 520

                         570
                  ....*....|..
gi 641744967 3088 PNGKLDRKALPA 3099
Cdd:PLN02246  521 PSGKILRKDLRA 532
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
 2632-2995 1.52e-16

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 86.12  E-value: 1.52e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVR--PDERVAI----CVErgldMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAK-P 2704
Cdd:cd05927     6 ISYKEVAERADNIGSALRSLGGKpaPASFVGIysinRPE----WIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEiS 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2705 VALISqsahlgimnGSLPVILLDD----GETRPFDNEPDTPLDarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVNF 2780
Cdd:cd05927    82 IVFCD---------AGVKVYSLEEfeklGKKNKVPPPPPKPED----------LATICYTSGTTGNPKGVMLTHGNIVSN 142

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2781 LCSMRKEPGIA----QEDVLLGVTSLS--FDISILEIFlpLLNGA---------RLILATQAQAADAQQLAM--LIER-H 2842
Cdd:cd05927   143 VAGVFKILEILnkinPTDVYISYLPLAhiFERVVEALF--LYHGAkigfysgdiRLLLDDIKALKPTVFPGVprVLNRiY 220

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2843 A--VSFMQATPSTWRMLVelrDFAL-------------PPGF---------KALCGGE-------ALP------ENLATA 2885
Cdd:cd05927   221 DkiFNKVQAKGPLKRKLF---NFALnyklaelrsgvvrASPFwdklvfnkiKQALGGNvrlmltgSAPlspevlEFLRVA 297

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2886 LlqKVTTLWNlYGPTETTIWSTLN--GLTTPTpYIGHPIANTQI----------YILDAQGRvvplgvaGEIHIAGAGVV 2953
Cdd:cd05927   298 L--GCPVLEG-YGQTECTAGATLTlpGDTSVG-HVGGPLPCAEVklvdvpemnyDAKDPNPR-------GEVCIRGPNVF 366

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|..
gi 641744967 2954 RGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTL 2995
Cdd:cd05927   367 SGYYKDPEKTAEALDEDGW--------LHTGDIGEWLPNGTL 400
PLN02654 PLN02654
acetate-CoA ligase
 1543-2021 1.71e-16

acetate-CoA ligase


Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 86.49  E-value: 1.71e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1543 DTTAVLFE------DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAER 1616
Cdd:PLN02654  104 DKIAIYWEgnepgfDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAES 183

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1617 LAYMLDDASPVALLT----QANQRALLTGDVPRILLDTADFSHLS---------------EDNPHVPG------------ 1665
Cdd:PLN02654  184 LAQRIVDCKPKVVITcnavKRGPKTINLKDIVDAALDESAKNGVSvgicltyenqlamkrEDTKWQEGrdvwwqdvvpny 263

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1666 --------LDAHHLAYVIYTSGSTGKPKGVMNSHRALcnrLVWMQNTYRltpddrvlqktpFSFDVSVWEFFW------- 1730
Cdd:PLN02654  264 ptkcevewVDAEDPLFLLYTSGSTGKPKGVLHTTGGY---MVYTATTFK------------YAFDYKPTDVYWctadcgw 328

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1731 ----------PLLYGAR-LVMARPDGHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWAD---ADCACDSLRRVICSGEA 1796
Cdd:PLN02654  329 itghsyvtygPMLNGATvLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDeyvTRHSRKSLRVLGSVGEP 408

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1797 LPAELQQRFFarfnaqlhNLYGPTEAAIDVTFW-------------ACQPDDHRSFVpigRPIANTQLYILDTLGQPVPL 1863
Cdd:PLN02654  409 INPSAWRWFF--------NVVGDSRCPISDTWWqtetggfmitplpGAWPQKPGSAT---FPFFGVQPVIVDEKGKEIEG 477

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1864 GVAGELHIGGV--GVARGYLNrpdlTAERFIPDPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEI 1941
Cdd:PLN02654  478 ECSGYLCVKKSwpGAFRTLYG----DHERYETTYF--KPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEV 551

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1942 EARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVtPDPADLRQQLGQHLAEYMvpGAFVTLDA------FPLTPNGK 2015
Cdd:PLN02654  552 ESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVEGV-PYSEELRKSLILTVRNQI--GAFAAPDKihwapgLPKTRSGK 628


                  ....*.
gi 641744967 2016 LDRKAL 2021
Cdd:PLN02654  629 IMRRIL 634
PLN02246 PLN02246
4-coumarate--CoA ligase
 499-965 2.09e-16

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 85.42  E-value: 2.09e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:PLN02246   51 YTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKASGAKLIITQS 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 AQVAQL-----NSTLPTVLLDTPA---------AAACPDTNPVVQgLHAAHLAYVIYTSGSTGRPKGVMVAHRNVI---- 640
Cdd:PLN02246  131 CYVDKLkglaeDDGVTVVTIDDPPegclhfselTQADENELPEVE-ISPDDVVALPYSSGTTGLPKGVMLTHKGLVtsva 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  641 --------NLatGLHT------LLALDHpsrI-ALNaSIVFDAsvknwiqLLSGHTLVLVPdalRADAHQLWRYFARHAV 705
Cdd:PLN02246  210 qqvdgenpNL--YFHSddvilcVLPMFH---IySLN-SVLLCG-------LRVGAAILIMP---KFEIGALLELIQRHKV 273

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  706 DLFDCTPvqlqwlldaglgsdpayqPAQVLIggeAISPAVWSR-LQS--------------LSDT---RFINV-----YG 762
Cdd:PLN02246  274 TIAPFVP------------------PIVLAI---AKSPVVEKYdLSSirmvlsgaaplgkeLEDAfraKLPNAvlgqgYG 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  763 PTECTVDATACVVDRTQPLPTigKPLA------NTRLYILD-------AQDQPvpigvtGELHIGGAGVARGYLHRPDLT 829
Cdd:PLN02246  333 MTEAGPVLAMCLAFAKEPFPV--KSGScgtvvrNAELKIVDpetgaslPRNQP------GEICIRGPQIMKGYLNDPEAT 404

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  830 AErfipdpfsadpaariykTGDLARWLPDGNIDYLGRND--F-------QIKVRGFRIEAGEIESRLLRCPGVQDAVVIA 900
Cdd:PLN02246  405 AN-----------------TIDKDGWLHTGDIGYIDDDDelFivdrlkeLIKYKGFQVAPAELEALLISHPSIADAAVVP 467

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967  901 REDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADY-MIPSAFVTlDALPLTPNGKLDRKALPA 965
Cdd:PLN02246  468 MKDEVAGEVPVAFVVRSNGSEITEDEIKQFVAKQVVFYkRIHKVFFV-DSIPKAPSGKILRKDLRA 532
AFD_CAR-like cd17632
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ...
 1536-1995 3.11e-16

adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.


Pssm-ID: 341287 [Multi-domain]  Cd Length: 588  Bit Score: 85.20  E-value: 3.11e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1536 DQAARTPDTTAVLFEDQHLTYDALNRRANQLAH-HLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPA 1614
Cdd:cd17632    50 TDPATGRTTLRLLPRFETITYAELWERVGAVAAaHDPEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASA 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1615 ERLAYMLDDASP------VALLTQANQRALLTGDVPRILLdtadFSHLSEDNPH-------------------------- 1662
Cdd:cd17632   130 AQLAPILAETEPrllavsAEHLDLAVEAVLEGGTPPRLVV----FDHRPEVDAHraalesarerlaavgipvttltliav 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1663 -----------VPGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKtpfsfdvsvwefFWP 1731
Cdd:cd17632   206 rgrdlppaplfRPEPDDDPLALLIYTSGSTGTPKGAMYTERLVATFWLKVSSIQDIRPPASITLN------------FMP 273

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1732 LLY-GARLVMARPDGHKDAAY------LAQLIERTGI---TTLHFVP---SMLQQ---------FVQWADADCACDSLR- 1788
Cdd:cd17632   274 MSHiAGRISLYGTLARGGTAYfaaasdMSTLFDDLALvrpTELFLVPrvcDMLFQryqaeldrrSVAGADAETLAERVKa 353

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1789 ---------RV---ICSGEALPAELQQRFFARFNAQLHNLYGPTEAAIDVTfwacqpdDHRSFVPigrPIANTQLYILDT 1856
Cdd:cd17632   354 elrervlggRLlaaVCGSAPLSAEMKAFMESLLDLDLHDGYGSTEAGAVIL-------DGVIVRP---PVLDYKLVDVPE 423

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1857 LG-----QPVPlgvAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGD-LARWLPDgSLEYLGRNDFQVK 1930
Cdd:cd17632   424 LGyfrtdRPHP---RGELLVKTDTLFPGYYKRPEVTAEVFDEDGF--------YRTGDvMAELGPD-RLVYVDRRNNVLK 491

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 1931 L-RGFRIELGEIEARLMQCPGVQEAVVVAredSPGDTRLVAYLCPQPGVTP--DPADLRQQLGQHLAE 1995
Cdd:cd17632   492 LsQGEFVTVARLEAVFAASPLVRQIFVYG---NSERAYLLAVVVPTQDALAgeDTARLRAALAESLQR 556
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
 2619-3097 3.11e-16

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 85.24  E-value: 3.11e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVF-----GEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPL------- 2686
Cdd:cd05970    30 YPDKLALVWcddagEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPAthqltak 109

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2687 DPTYPVERLrymldDAKPVALISQSAHLGIMNGSLPVILLDDGETRPFDNEPDTPLDARKQ------GLTPRH------- 2753
Cdd:cd05970   110 DIVYRIESA-----DIKMIVAIAEDNIPEEIEKAAPECPSKPKLVWVGDPVPEGWIDFRKLiknaspDFERPTansypcg 184

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2754 --LAYVIYTSGSTGKPKgvMVEHANM--VNFLCSMRKEPGIAQEDVLLGVTSLSFDISIL-EIFLPLLNGARLILATQAQ 2828
Cdd:cd05970   185 edILLVYFSSGTTGMPK--MVEHDFTypLGHIVTAKYWQNVREGGLHLTVADTGWGKAVWgKIYGQWIAGAAVFVYDYDK 262

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2829 AADAQqlaML--IERHAVSFMQATPSTWRMLV--ELRDFALPPGFKALCGGEALPENLATALLQKV-TTLWNLYGPTETT 2903
Cdd:cd05970   263 FDPKA---LLekLSKYGVTTFCAPPTIYRFLIreDLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTgIKLMEGFGQTETT 339

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2904 IW-STLNGLTTPTPYIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGA-----GVVRGYLGRPDLTAERFitdpFSGape 2977
Cdd:cd05970   340 LTiATFPWMEPKPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIRTSkgkpvGLFGGYYKDAEKTAEVW----HDG--- 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2978 arMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARchgvHDAVV-IAREDSPGDKR-------LVAYLL 3049
Cdd:cd05970   413 --YYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQ----HPAVLeCAVTGVPDPIRgqvvkatIVLAKG 486

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 641744967 3050 AQPDTVLEpADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05970   487 YEPSEELK-KELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEI 533
PaaK COG1541
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ...
 1677-1993 3.25e-16

Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];


Pssm-ID: 441150 [Multi-domain]  Cd Length: 423  Bit Score: 84.05  E-value: 3.25e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1677 TSGSTGKPKGVMNSHRALcnrLVWMQNTYR------LTPDDRVLqkTPFSFDVSVWefFWPLLYGARLV--MARPDGHKD 1748
Cdd:COG1541    91 SSGTTGKPTVVGYTRKDL---DRWAELFARslraagVRPGDRVQ--NAFGYGLFTG--GLGLHYGAERLgaTVIPAGGGN 163

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1749 AAYLAQLIERTGITTLHFVPSMLQQFVQWADA---DCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAAID 1825
Cdd:COG1541   164 TERQLRLMQDFGPTVLVGTPSYLLYLAEVAEEegiDPRDLSLKKGIFGGEPWSEEMRKEIEERWGIKAYDIYGLTEVGPG 243

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1826 VtFWACQ-------PDDHRsFVPIGRPiantqlyilDTlGQPVPLGVAGELHIGGvgvargylnrpdLTAERFipdPFIN 1898
Cdd:COG1541   244 V-AYECEaqdglhiWEDHF-LVEIIDP---------ET-GEPVPEGEEGELVVTT------------LTKEAM---PLIR 296

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1899 qpgarlYKTGDLARWLPDGS--------LEY-LGRNDFQVKLRGFRIELGEIEARLMQCPGVQEA--VVVAREDspGDTR 1967
Cdd:COG1541   297 ------YRTGDLTRLLPEPCpcgrthprIGRiLGRADDMLIIRGVNVFPSQIEEVLLRIPEVGPEyqIVVDREG--GLDE 368

                         330       340
                  ....*....|....*....|....*.
gi 641744967 1968 LVAYLCPQPGVtpDPADLRQQLGQHL 1993
Cdd:COG1541   369 LTVRVELAPGA--SLEALAEAIAAAL 392
PRK00174 PRK00174
acetyl-CoA synthetase; Provisional
 1543-2021 4.43e-16

acetyl-CoA synthetase; Provisional


Pssm-ID: 234677 [Multi-domain]  Cd Length: 637  Bit Score: 84.81  E-value: 4.43e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1543 DTTAVLFE------DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAER 1616
Cdd:PRK00174   82 DKVAIIWEgddpgdSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVVFGGFSAEA 161

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1617 LAYMLDDASPVALLT-------------QAN-QRALLTGD-------VPRILLDTA-----DF------SHLSEDNPHVP 1664
Cdd:PRK00174  162 LADRIIDAGAKLVITadegvrggkpiplKANvDEALANCPsvekvivVRRTGGDVDwvegrDLwwhelvAGASDECEPEP 241

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1665 gLDAHHLAYVIYTSGSTGKPKGVMNSHRALcnrLVWMQNTYRLTpddrvlqktpfsFDVSVWEFFW-------------- 1730
Cdd:PRK00174  242 -MDAEDPLFILYTSGSTGKPKGVLHTTGGY---LVYAAMTMKYV------------FDYKDGDVYWctadvgwvtghsyi 305

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1731 ---PLLYGARLVM--ARPDgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQWADA-----DCAcdSLRrVICS-GEALPA 1799
Cdd:PRK00174  306 vygPLANGATTLMfeGVPN-YPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEhpkkyDLS--SLR-LLGSvGEPINP 381

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1800 ELQQRFfarfnaqlHNLYGPTEAAIDVTFW--------------AcqpddhrsfVPI-----GRPIANTQLYILDTLGQP 1860
Cdd:PRK00174  382 EAWEWY--------YKVVGGERCPIVDTWWqtetggimitplpgA---------TPLkpgsaTRPLPGIQPAVVDEEGNP 444

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1861 VPLGVAGELHIGGV--GVARGYLNRPdltaERFIPDPFINQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIEL 1938
Cdd:PRK00174  445 LEGGEGGNLVIKDPwpGMMRTIYGDH----ERFVKTYFSTFKG--MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGT 518

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1939 GEIEARLMQCPGVQEAVVVARedsPGDTR---LVAYLCPQPGVTPDPAdLRQQLGQHLAEYMVPGA------FVtlDAFP 2009
Cdd:PRK00174  519 AEIESALVAHPKVAEAAVVGR---PDDIKgqgIYAFVTLKGGEEPSDE-LRKELRNWVRKEIGPIAkpdviqFA--PGLP 592

                         570
                  ....*....|..
gi 641744967 2010 LTPNGKLDRKAL 2021
Cdd:PRK00174  593 KTRSGKIMRRIL 604
AMP-binding_C pfam13193
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ...
 882-957 5.28e-16

AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.


Pssm-ID: 463804 [Multi-domain]  Cd Length: 76  Bit Score: 74.89  E-value: 5.28e-16
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967   882 EIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGK 957
Cdd:pfam13193    1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
 1554-1976 5.76e-16

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 84.19  E-value: 5.76e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVK--PDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLT 1631
Cdd:cd05927     6 ISYKEVAERADNIGSALRSLGGKpaPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVFC 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1632 QAnqralltgDVPRILLDtaDFSHLSEDN--PHVPGlDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLV----WMQNTY 1705
Cdd:cd05927    86 DA--------GVKVYSLE--EFEKLGKKNkvPPPPP-KPEDLATICYTSGTTGNPKGVMLTHGNIVSNVAgvfkILEILN 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1706 RLTPDDRVLQKTPFS--FDVSVWEFFwpLLYGARLvmarpdG--HKDAAYLA---------------QLIER--TGITTL 1764
Cdd:cd05927   155 KINPTDVYISYLPLAhiFERVVEALF--LYHGAKI------GfySGDIRLLLddikalkptvfpgvpRVLNRiyDKIFNK 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1765 HFVPSMLQQFVqwadADCACDS----LR--------------------------RVICSGEA-LPAELQQRFFARFNAQL 1813
Cdd:cd05927   227 VQAKGPLKRKL----FNFALNYklaeLRsgvvraspfwdklvfnkikqalggnvRLMLTGSApLSPEVLEFLRVALGCPV 302

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1814 HNLYGPTE--AAIDVTFwacqPDDHrSFVPIGRPIANTQLYILDtlgqpVP-LGV-------AGELHIGGVGVARGYLNR 1883
Cdd:cd05927   303 LEGYGQTEctAGATLTL----PGDT-SVGHVGGPLPCAEVKLVD-----VPeMNYdakdpnpRGEVCIRGPNVFSGYYKD 372

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1884 PDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYLGR--NDFqvKL-RGFRIELGEIEARLMQCPGVQEAVVVare 1960
Cdd:cd05927   373 PEKTAEALDEDGW--------LHTGDIGEWLPNGTLKIIDRkkNIF--KLsQGEYVAPEKIENIYARSPFVAQIFVY--- 439

                         490
                  ....*....|....*....
gi 641744967 1961 dspGD---TRLVAYLCPQP 1976
Cdd:cd05927   440 ---GDslkSFLVAIVVPDP 455
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
 475-965 5.83e-16

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 84.31  E-value: 5.83e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  475 IQQRFEAQAEQTpeAIAVLFEDQHLTYRELNRRANQLAHHLIALGvQPDDR--VALCVERSLEMMVGLLGILKAGAAYVP 552
Cdd:PRK13388    5 IAQLLRDRAGDD--TIAVRYGDRTWTWREVLAEAAARAAALIALA-DPDRPlhVGVLLGNTPEMLFWLAAAALGGYVLVG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  553 MDPAYPAERLAYILDDAAPVALLTQSAQVAQLN----STLPTVLLDTPA----AAACPDTNPVVQgLHAAHLAYVIYTSG 624
Cdd:PRK13388   82 LNTTRRGAALAADIRRADCQLLVTDAEHRPLLDgldlPGVRVLDVDTPAyaelVAAAGALTPHRE-VDAMDPFMLIFTSG 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  625 STGRPKGVMVAHRNVINLATGLHTLLALdHPSRIALNASIVF--DASVKNW-IQLLSGHTLVLVPdalRADAHQLWRYFA 701
Cdd:PRK13388  161 TTGAPKAVRCSHGRLAFAGRALTERFGL-TRDDVCYVSMPLFhsNAVMAGWaPAVASGAAVALPA---KFSASGFLDDVR 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  702 RHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAiSPAVWSRLQSLSDTRFINVYGPTEctvDATACVVDRTQPL 781
Cdd:PRK13388  237 RYGATYFNYVGKPLAYILATPERPDDADNPLRVAFGNEA-SPRDIAEFSRRFGCQVEDGYGSSE---GAVIVVREPGTPP 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  782 PTIGKPLANTRLYILDA-QDQPV-----------PIGVTGEL-HIGGAGVARGYLHRPDLTAERFipdpfsadpAARIYK 848
Cdd:PRK13388  313 GSIGRGAPGVAIYNPETlTECAVarfdahgallnADEAIGELvNTAGAGFFEGYYNNPEATAERM---------RHGMYW 383

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  849 TGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALR 928
Cdd:PRK13388  384 SGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDPDAFA 463

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 641744967  929 QQLAAS--LADYMIPSAFVTLDALPLTPNGKLDRKALPA 965
Cdd:PRK13388  464 AFLAAQpdLGTKAWPRYVRIAADLPSTATNKVLKRELIA 502
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
 1842-2021 7.02e-16

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 84.10  E-value: 7.02e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1842 IGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEY 1921
Cdd:PRK12492  388 VGIPVPGTALKVIDDDGNELPLGERGELCIKGPQVMKGYWQQPEATAEALDAEGW--------FKTGDIAVIDPDGFVRI 459

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1922 LGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQ-PGVTPDpaDLRQQLGQHLAEYMVPG 2000
Cdd:PRK12492  460 VDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARdPGLSVE--ELKAYCKENFTGYKVPK 537

                         170       180
                  ....*....|....*....|.
gi 641744967 2001 AFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK12492  538 HIVLRDSLPMTPVGKILRREL 558
FATP_chFAT1_like cd05937
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ...
 1549-1957 8.24e-16

Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.


Pssm-ID: 341260 [Multi-domain]  Cd Length: 468  Bit Score: 83.25  E-value: 8.24e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1549 FEDQHLTYDALNRRANQLAHHLID-LGVKPDDRIAICVERSLDMVIGLLAILKAGAAyvpldpgyPAeRLAYMLDDASPV 1627
Cdd:cd05937     1 FEGKTWTYSETYDLVLRYAHWLHDdLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAA--------PA-FINYNLSGDPLI 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1628 ALLTQANQRALL-TGDVPRILldtadfshlsednphvpgldahhlayvIYTSGSTGKPKGVMNSHRaLCNRLVW-MQNTY 1705
Cdd:cd05937    72 HCLKLSGSRFVIvDPDDPAIL---------------------------IYTSGTTGLPKAAAISWR-RTLVTSNlLSHDL 123

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1706 RLTPDDRVLQKTP-FSFDVSVWEFFWPLLYGARLVMAR--------PDGHKDAAYLAQLIERTGITTLHFVPSMLqqfvq 1776
Cdd:cd05937   124 NLKNGDRTYTCMPlYHGTAAFLGACNCLMSGGTLALSRkfsasqfwKDVRDSGATIIQYVGELCRYLLSTPPSPY----- 198

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1777 wadadcacDSLRRVICS-GEALPAELQQRFFARFN-AQLHNLYGPTEAAIdvTFWACQPDD-------HRSfvPIGRPIA 1847
Cdd:cd05937   199 --------DRDHKVRVAwGNGLRPDIWERFRERFNvPEIGEFYAATEGVF--ALTNHNVGDfgagaigHHG--LIRRWKF 266

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1848 NTQLYI----LDTlGQP-----------VPLGVAGE----LHIGGVGVARGYLNRPDLTAERFIPDPFinQPGARLYKTG 1908
Cdd:cd05937   267 ENQVVLvkmdPET-DDPirdpktgfcvrAPVGEPGEmlgrVPFKNREAFQGYLHNEDATESKLVRDVF--RKGDIYFRTG 343

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967 1909 DLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVV 1957
Cdd:cd05937   344 DLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEANVY 392
A_NRPS_MycA_like cd05908
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ...
 1668-1958 1.00e-15

The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341234 [Multi-domain]  Cd Length: 499  Bit Score: 83.31  E-value: 1.00e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1668 AHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWEF-FWPLLYGA-------RLV 1739
Cdd:cd05908   105 ADELAFIQFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTHDMGLIAFhLAPLIAGMnqylmptRLF 184

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1740 MARPdghkdAAYLAQLIER--TGITTLHFVPS-MLQQFVQWADADCACDSLRRVICSGEALPAELQQRFFARFNA----- 1811
Cdd:cd05908   185 IRRP-----ILWLKKASEHkaTIVSSPNFGYKyFLKTLKPEKANDWDLSSIRMILNGAEPIDYELCHEFLDHMSKyglkr 259

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1812 -QLHNLYGPTEAAIDVTFWACQPD------DHR-------------------SFVPIGRPIANTQLYILDTLGQPVPLGV 1865
Cdd:cd05908   260 nAILPVYGLAEASVGASLPKAQSPfktitlGRRhvthgepepevdkkdseclTFVEVGKPIDETDIRICDEDNKILPDGY 339

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1866 AGELHIGGVGVARGYLNRPDLTAERFIPDPFInqpgarlyKTGDLArWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARL 1945
Cdd:cd05908   340 IGHIQIRGKNVTPGYYNNPEATAKVFTDDGWL--------KTGDLG-FIRNGRLVITGREKDIIFVNGQNVYPHDIERIA 410

                         330
                  ....*....|...
gi 641744967 1946 MQCPGVQEAVVVA 1958
Cdd:cd05908   411 EELEGVELGRVVA 423
FATP_chFAT1_like cd05937
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ...
 494-963 1.27e-15

Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.


Pssm-ID: 341260 [Multi-domain]  Cd Length: 468  Bit Score: 82.87  E-value: 1.27e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  494 FEDQHLTYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVGLLGILKAGAAyvpmdPAYpaerLAYILDDAAPV 572
Cdd:cd05937     1 FEGKTWTYSETYDLVLRYAHWLHdDLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAA-----PAF----INYNLSGDPLI 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  573 ALLTQSaqvaqlNSTLPTVLLDTPAAaacpdtnpvvqglhaahlayVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLAL 652
Cdd:cd05937    72 HCLKLS------GSRFVIVDPDDPAI--------------------LIYTSGTTGLPKAAAISWRRTLVTSNLLSHDLNL 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  653 DHPSRI--------ALNASIVFDASvknwiqLLSGHTLVLVPdalRADAHQLWRyfarhAVDLFDCTPVQ-----LQWLL 719
Cdd:cd05937   126 KNGDRTytcmplyhGTAAFLGACNC------LMSGGTLALSR---KFSASQFWK-----DVRDSGATIIQyvgelCRYLL 191

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  720 DAGLGSDPAYQPAQVLIGgEAISPAVWSRLQSlsdtRFiNV------YGPTECTV--------DATACVVDRTQPL---- 781
Cdd:cd05937   192 STPPSPYDRDHKVRVAWG-NGLRPDIWERFRE----RF-NVpeigefYAATEGVFaltnhnvgDFGAGAIGHHGLIrrwk 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  782 ----PTIGKPLANTRLYILDAQD---QPVPIGVTGE----LHIGGAGVARGYLHRPDLTAERFIPDPFSADPAarIYKTG 850
Cdd:cd05937   266 fenqVVLVKMDPETDDPIRDPKTgfcVRAPVGEPGEmlgrVPFKNREAFQGYLHNEDATESKLVRDVFRKGDI--YFRTG 343

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  851 DLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAALRQQ 930
Cdd:cd05937   344 DLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEANVYGVKVPGHDGRAGCAAITLEESSAVPTEFTKS 423

                         490       500       510
                  ....*....|....*....|....*....|....*...
gi 641744967  931 LAASLADYMIPSAFVTL-----DALPLTPNGKLDRKAL 963
Cdd:cd05937   424 LLASLARKNLPSYAVPLflrltEEVATTDNHKQQKGVL 461
FCS cd05921
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ...
 2633-3070 1.38e-15

Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.


Pssm-ID: 341245 [Multi-domain]  Cd Length: 561  Bit Score: 83.25  E-value: 1.38e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2633 SYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPV-----ERLRYMLDDAKPVAL 2707
Cdd:cd05921    27 TYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVSPAYSLmsqdlAKLKHLFELLKPGLV 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2708 ISQSAHL------GIMNGSLPVI----LLDDGETRPFDNEPDTP----LDARKQGLTPRHLAYVIYTSGSTGKPKGVMVE 2773
Cdd:cd05921   107 FAQDAAPfaralaAIFPLGTPLVvsrnAVAGRGAISFAELAATPptaaVDAAFAAVGPDTVAKFLFTSGSTGLPKAVINT 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2774 HANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISI---LEIFLPLLNGARLILATQAQAADAQQLAM--LIERHAVSFMQ 2848
Cdd:cd05921   187 QRMLCANQAMLEQTYPFFGEEPPVLVDWLPWNHTFggnHNFNLVLYNGGTLYIDDGKPMPGGFEETLrnLREISPTVYFN 266

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2849 AtPSTWRMLVEL--RDFALPPGF----KALC-GGEALPENLATAlLQKVT--------TLWNLYGPTETTIWSTL-NGLT 2912
Cdd:cd05921   267 V-PAGWEMLVAAleKDEALRRRFfkrlKLMFyAGAGLSQDVWDR-LQALAvatvgeriPMMAGLGATETAPTATFtHWPT 344

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2913 TPTPYIGHPIANTQIyildaqgRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWL-- 2990
Cdd:cd05921   345 ERSGLIGLPAPGTEL-------KLVPSGGKYEVRVKGPNVTPGYWRQPELTAQAFDEEGF--------YCLGDAAKLAdp 409

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2991 --PDGTLEYLGR--NDFQVKvRGFRIELGEIETRL-ARCHG-VHDAVVIA--RE--------DSPGDKRLVAYLLAQPDT 3054
Cdd:cd05921   410 ddPAKGLVFDGRvaEDFKLA-SGTWVSVGPLRARAvAACAPlVHDAVVAGedRAevgalvfpDLLACRRLVGLQEASDAE 488

                         490
                  ....*....|....*.
gi 641744967 3055 VLEPADLRQRLSEGVA 3070
Cdd:cd05921   489 VLRHAKVRAAFRDRLA 504
A_NRPS_MycA_like cd05908
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ...
 2751-3029 1.50e-15

The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341234 [Multi-domain]  Cd Length: 499  Bit Score: 82.54  E-value: 1.50e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2751 PRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFL-PLLNGARLILATQAQA 2829
Cdd:cd05908   105 ADELAFIQFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTHDMGLIAFHLaPLIAGMNQYLMPTRLF 184

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2830 ADAQQLAML-IERHAVSFMQATPSTWRMLVE------LRDFALPPGFKALCGGEALPENLATALLQ-------KVTTLWN 2895
Cdd:cd05908   185 IRRPILWLKkASEHKATIVSSPNFGYKYFLKtlkpekANDWDLSSIRMILNGAEPIDYELCHEFLDhmskyglKRNAILP 264

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2896 LYGPTETTIWSTLNGLTTP--TPYIGH---------------------------PIANTQIYILDAQGRVVPLGVAGEIH 2946
Cdd:cd05908   265 VYGLAEASVGASLPKAQSPfkTITLGRrhvthgepepevdkkdsecltfvevgkPIDETDIRICDEDNKILPDGYIGHIQ 344

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2947 IAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGrWLPDGTLEYLGRNDFQVKVRGFRIELGEIET------- 3019
Cdd:cd05908   345 IRGKNVTPGYYNNPEATAKVFTDDGW--------LKTGDLG-FIRNGRLVITGREKDIIFVNGQNVYPHDIERiaeeleg 415

                         330
                  ....*....|....
gi 641744967 3020 ----RLARChGVHD 3029
Cdd:cd05908   416 velgRVVAC-GVNN 428
PLN02479 PLN02479
acetate-CoA ligase
 621-965 1.77e-15

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 82.97  E-value: 1.77e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  621 YTSGSTGRPKGVMVAHRNVInlatglhtLLALDHPSRIALNASIVF---------DASVKNW-IQLLSGHTLVLvpdaLR 690
Cdd:PLN02479  202 YTSGTTASPKGVVLHHRGAY--------LMALSNALIWGMNEGAVYlwtlpmfhcNGWCFTWtLAALCGTNICL----RQ 269

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  691 ADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSD--PAYQPAQVLIGGEAISPAVWSRLqSLSDTRFINVYGPTECTV 768
Cdd:PLN02479  270 VTAKAIYSAIANYGVTHFCAAPVVLNTIVNAPKSETilPLPRVVHVMTAGAAPPPSVLFAM-SEKGFRVTHTYGLSETYG 348

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  769 DATACVvdrTQP----LPTIGKPLANTR----------LYILDAQD-QPVPI-GVT-GELHIGGAGVARGYLHRPDLTAE 831
Cdd:PLN02479  349 PSTVCA---WKPewdsLPPEEQARLNARqgvryiglegLDVVDTKTmKPVPAdGKTmGEIVMRGNMVMKGYLKNPKANEE 425

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  832 RFipdpfsadpAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLV 911
Cdd:PLN02479  426 AF---------ANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPC 496

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967  912 AYLCARPDAELH-PAALRQQLAA----SLADYMIPSAfVTLDALPLTPNGKLDRKALPA 965
Cdd:PLN02479  497 AFVTLKPGVDKSdEAALAEDIMKfcreRLPAYWVPKS-VVFGPLPKTATGKIQKHVLRA 554
hsFATP4_like cd05939
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ...
 496-963 1.81e-15

Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341262 [Multi-domain]  Cd Length: 474  Bit Score: 82.09  E-value: 1.81e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  496 DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALL 575
Cdd:cd05939     1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  576 TQsaqvaqlnstlptvlLDTPAAAACPDTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALdHP 655
Cdd:cd05939    81 FN---------------LLDPLLTQSSTEPPSQDDVNFRDKLFYIYTSGTTGLPKAAVIVHSRYYRIAAGAYYAFGM-RP 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  656 SRIALN--------ASIVFDASVknwiqLLSGHTLVLVPdalRADAHQLWRYFARHavdlfDCTPVQL-----QWLLDAG 722
Cdd:cd05939   145 EDVVYDclplyhsaGGIMGVGQA-----LLHGSTVVIRK---KFSASNFWDDCVKY-----NCTIVQYigeicRYLLAQP 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  723 LgsDPAYQPAQV-LIGGEAISPAVWSRLQSlsdtRFiNV------YGPTECT-----VDAT--ACVVdrtqpLPTIGKPL 788
Cdd:cd05939   212 P--SEEEQKHNVrLAVGNGLRPQIWEQFVR----RF-GIpqigefYGATEGNsslvnIDNHvgACGF-----NSRILPSV 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  789 ANTRLYILDAqdqpvpigVTGELHIGGAGVA------------------------RGYLHRPDlTAERFIPDPF-SADPA 843
Cdd:cd05939   280 YPIRLIKVDE--------DTGELIRDSDGLCipcqpgepgllvgkiiqndplrrfDGYVNEGA-TNKKIARDVFkKGDSA 350

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  844 ariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELH 923
Cdd:cd05939   351 ---FLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVYGVEVPGVEGRAGMAAIVDPERKVD 427

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 641744967  924 PAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05939   428 LDRFSAVLAKSLPPYARPQFIRLLPEVDKTGTFKLQKTDL 467
PRK08308 PRK08308
acyl-CoA synthetase; Validated
 496-970 2.10e-15

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 81.62  E-value: 2.10e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  496 DQHLTYRELNRRANQLAHHLIaLGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYP---AERLAyilDDAAPV 572
Cdd:PRK08308    6 DEEYSKSDFDLRLQRYEEMEQ-FQEAAGNRFAVCLKDPFDIITLVFFLKEKGASVLPIHPDTPkeaAIRMA---KRAGCH 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  573 ALLTQSAQvaqlnstlpTVLLDTPAAAACPDTnpVVQglhaahlayviYTSGSTGRPKGVmvaHRNVINLATGLHTLLAL 652
Cdd:PRK08308   82 GLLYGESD---------FTKLEAVNYLAEEPS--LLQ-----------YSSGTTGEPKLI---RRSWTEIDREIEAYNEA 136

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  653 dhPSRIALNASIVFdASVKNWIQLLSGhtlVLVpdALRADA--HQLWRYFARHAVDLFDCTPVQLqwlldagLGSDPA-- 728
Cdd:PRK08308  137 --LNCEQDETPIVA-CPVTHSYGLICG---VLA--ALTRGSkpVIITNKNPKFALNILRNTPQHI-------LYAVPLml 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  729 YQPAQVLIGGEAIS---------PAVW-SRLQSLSdTRFINVYGPTEctvdaTACVV---DRTQPLpTIGKPLANTRLYI 795
Cdd:PRK08308  202 HILGRLLPGTFQFHavmtsgtplPEAWfYKLRERT-TYMMQQYGCSE-----AGCVSicpDMKSHL-DLGNPLPHVSVSA 274

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  796 LDAQDQPVPIGVTgelhiggagvargylhrpdlTAERfipdpfsadpaaRIYkTGDLARWLPDGNIDYLGRNDFQIKVRG 875
Cdd:PRK08308  275 GSDENAPEEIVVK--------------------MGDK------------EIF-TKDLGYKSERGTLHFMGRMDDVINVSG 321

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  876 FRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCArpDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPN 955
Cdd:PRK08308  322 LNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVIS--HEEIDPVQLREWCIQHLAPYQVPHEIESVTEIPKNAN 399

                         490
                  ....*....|....*
gi 641744967  956 GKLDRKALPAPDQTA 970
Cdd:PRK08308  400 GKVSRKLLELGEVTA 414
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
 500-963 2.44e-15

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 82.11  E-value: 2.44e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  500 TYRELNRRANQLAHHLIALGVQPDDRVALC---VERSLEMMVGLLGIlkaGAAYVPMDPAYPAERLAYILDDAAPVALLT 576
Cdd:PRK06018   41 TYAQIHDRALKVSQALDRDGIKLGDRVATIawnTWRHLEAWYGIMGI---GAICHTVNPRLFPEQIAWIINHAEDRVVIT 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  577 QSAQV---AQLNSTLPTV-----LLDtpaAAACPDT---NPVV--QGLHAAH------------LAYVIYTSGSTGRPKG 631
Cdd:PRK06018  118 DLTFVpilEKIADKLPSVeryvvLTD---AAHMPQTtlkNAVAyeEWIAEADgdfawktfdentAAGMCYTSGTTGDPKG 194

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  632 VMVAHR-NVinlatgLHTLLALDHPSRIALNASIV------FDASvkNWIQLLS----GHTLVLvPDAlRADAHQLWRYF 700
Cdd:PRK06018  195 VLYSHRsNV------LHALMANNGDALGTSAADTMlpvvplFHAN--SWGIAFSapsmGTKLVM-PGA-KLDGASVYELL 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  701 ARHAVDLFDCTPVQLQWLLDAgLGSDPAYQP--AQVLIGGEAISPAVWSRLQSLsDTRFINVYGPTEctvdatacvvdrT 778
Cdd:PRK06018  265 DTEKVTFTAGVPTVWLMLLQY-MEKEGLKLPhlKMVVCGGSAMPRSMIKAFEDM-GVEVRHAWGMTE------------M 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  779 QPLPTIGK---PLANT----RLYILDAQDQPvPIGV------------------TGELHIGGAGVARGYLHrpdltAERF 833
Cdd:PRK06018  331 SPLGTLAAlkpPFSKLpgdaRLDVLQKQGYP-PFGVemkitddagkelpwdgktFGRLKVRGPAVAAAYYR-----VDGE 404

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  834 IPDpfsadpAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAY 913
Cdd:PRK06018  405 ILD------DDGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLI 478

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 641744967  914 LCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:PRK06018  479 VQLKPGETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTAL 528
PRK12476 PRK12476
putative fatty-acid--CoA ligase; Provisional
 1539-2020 3.07e-15

putative fatty-acid--CoA ligase; Provisional


Pssm-ID: 171527 [Multi-domain]  Cd Length: 612  Bit Score: 82.10  E-value: 3.07e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1539 ARTPDTTAVlfedqHLTYDALNRRANQLAHHLIDLgVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLD----PGYpA 1614
Cdd:PRK12476   59 SHSAAGCAV-----ELTWTQLGVRLRAVGARLQQV-AGPGDRVAILAPQGIDYVAGFFAAIKAGTIAVPLFapelPGH-A 131

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1615 ERLAYMLDDASPVALLTQANQ--------RALLTGDVPRILLDTADFSHLSEDNPHVPgLDAHHLAYVIYTSGSTGKPKG 1686
Cdd:PRK12476  132 ERLDTALRDAEPTVVLTTTAAaeavegflRNLPRLRRPRVIAIDAIPDSAGESFVPVE-LDTDDVSHLQYTSGSTRPPVG 210

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1687 VMNSHRALCNRLVWM-QNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLVMARPDG--HKDAAYLAQLIErtGITT 1763
Cdd:PRK12476  211 VEITHRAVGTNLVQMiLSIDLLDRNTHGVSWLPLYHDMGLSMIGFPAVYGGHSTLMSPTAfvRRPQRWIKALSE--GSRT 288

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1764 LHFVPSMLQQFVQWA-----DADCACDSLRRV--ICSGEALPAELQQRF---FARFN---AQLHNLYGPTEAAIDVTFWA 1830
Cdd:PRK12476  289 GRVVTAAPNFAYEWAaqrglPAEGDDIDLSNVvlIIGSEPVSIDAVTTFnkaFAPYGlprTAFKPSYGIAEATLFVATIA 368

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1831 CQ---------------------PDDHRSFVP---IGRPIANTQLYILD-TLGQPVPLGVAGELHIGGVGVARGYLNRPD 1885
Cdd:PRK12476  369 PDaepsvvyldreqlgagravrvAADAPNAVAhvsCGQVARSQWAVIVDpDTGAELPDGEVGEIWLHGDNIGRGYWGRPE 448

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1886 LTAErfipdPFINQPGARL---------------YKTGDLARWLpDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQC-- 1948
Cdd:PRK12476  449 ETER-----TFGAKLQSRLaegshadgaaddgtwLRTGDLGVYL-DGELYITGRIADLIVIDGRNHYPQDIEATVAEAsp 522

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1949 ------------PGV--QEAVVVArEDSPGDTRLVaylcPQPGVTPDPADLRQQLGQHLAEYMvpgaFVTLDAFPLTPNG 2014
Cdd:PRK12476  523 mvrrgyvtaftvPAEdnERLVIVA-ERAAGTSRAD----PAPAIDAIRAAVSRRHGLAVADVR----LVPAGAIPRTTSG 593


                  ....*.
gi 641744967 2015 KLDRKA 2020
Cdd:PRK12476  594 KLARRA 599
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
 1552-1957 3.23e-15

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 81.75  E-value: 3.23e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1552 QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLT 1631
Cdd:cd05932     5 VEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFV 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1632 -----QANQRALLTGDVPRILL---DTADFSHLSED-----NPHV--PGLDAHHLAYVIYTSGSTGKPKGVMNSHRAL-- 1694
Cdd:cd05932    85 gklddWKAMAPGVPEGLISISLpppSAANCQYQWDDliaqhPPLEerPTRFPEQLATLIYTSGTTGQPKGVMLTFGSFaw 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1695 -CNRLVwmqNTYRLTPDDRVLQKTPFSFDVSVWEFFWPLLYGARLV--------------MARPDGHKDAAYLAQL---- 1755
Cdd:cd05932   165 aAQAGI---EHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVafaesldtfvedvqRARPTLFFSVPRLWTKfqqg 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1756 -IERTGITTLH------FVPSMLQQFVQwadADCACDSLRRVICSGEALPAELQQrFFARFNAQLHNLYGPTEAAIDVTF 1828
Cdd:cd05932   242 vQDKIPQQKLNlllkipVVNSLVKRKVL---KGLGLDQCRLAGCGSAPVPPALLE-WYRSLGLNILEAYGMTENFAYSHL 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1829 waCQPDDHRsfvpIGrpiantqlyildTLGQPVP-----LGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFInqpgar 1903
Cdd:cd05932   318 --NYPGRDK----IG------------TVGNAGPgvevrISEDGEILVRSPALMMGYYKDPEATAEAFTADGFL------ 373

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1904 lyKTGDLARWLPDGSLEYLGR-NDFQVKLRGFRIELGEIEARLMQCPGVQEAVVV 1957
Cdd:cd05932   374 --RTGDKGELDADGNLTITGRvKDIFKTSKGKYVAPAPIENKLAEHDRVEMVCVI 426
PRK03584 PRK03584
acetoacetate--CoA ligase;
485-958 3.49e-15

acetoacetate--CoA ligase;


Pssm-ID: 235134 [Multi-domain]  Cd Length: 655  Bit Score: 82.15  E-value: 3.49e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  485 QTPEAIAVLF--EDQH---LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPA 559
Cdd:PRK03584   96 RRDDRPAIIFrgEDGPrreLSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGV 175

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  560 ----ERLAYIlddaAPVALLT------------QSAQVAQLNSTLPTV--------LLDTPAAAACPDTNP---VVQGLH 612
Cdd:PRK03584  176 qgvlDRFGQI----EPKVLIAvdgyryggkafdRRAKVAELRAALPSLehvvvvpyLGPAAAAAALPGALLwedFLAPAE 251

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  613 AAHLA----------YVIYTSGSTGRPKGVMVAHRNVInL----ATGLHTLLaldHPSrialnaSIVFDASVKNWIQ--- 675
Cdd:PRK03584  252 AAELEfepvpfdhplWILYSSGTTGLPKCIVHGHGGIL-LehlkELGLHCDL---GPG------DRFFWYTTCGWMMwnw 321

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  676 ----LLSGHTLVLV------PDALRadahqLWRYFARHAVDLFDCTPVQLQWLLDAGLgsdpayQPAQ---------VLI 736
Cdd:PRK03584  322 lvsgLLVGATLVLYdgspfyPDPNV-----LWDLAAEEGVTVFGTSAKYLDACEKAGL------VPGEthdlsalrtIGS 390

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  737 GG------------EAISPAVWsrLQSLSdtrfinvyGPTectvDATACVV--DRTQPL-------PTIGkplanTRLYI 795
Cdd:PRK03584  391 TGsplppegfdwvyEHVKADVW--LASIS--------GGT----DICSCFVggNPLLPVyrgeiqcRGLG-----MAVEA 451

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  796 LDAQDQPVpIGVTGELhiggagVAR--------GYLHRPDltAERFIPDPFSADPAarIYKTGDLARWLPDGNIDYLGRN 867
Cdd:PRK03584  452 WDEDGRPV-VGEVGEL------VCTkpfpsmplGFWNDPD--GSRYRDAYFDTFPG--VWRHGDWIEITEHGGVVIYGRS 520

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  868 DFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPA---ALRQQLAASLADYMIPSAF 944
Cdd:PRK03584  521 DATLNRGGVRIGTAEIYRQVEALPEVLDSLVIGQEWPDGDVRMPLFVVLAEGVTLDDAlraRIRTTIRTNLSPRHVPDKI 600

                         570
                  ....*....|....
gi 641744967  945 VTLDALPLTPNGKL 958
Cdd:PRK03584  601 IAVPDIPRTLSGKK 614
PRK07769 PRK07769
long-chain-fatty-acid--CoA ligase; Validated
 468-866 3.61e-15

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 181109 [Multi-domain]  Cd Length: 631  Bit Score: 82.08  E-value: 3.61e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  468 DFPREMLIQQRFEAQAEQTPEAIAVLFED---------QHLTYRELNRRANQLAHHLIALGvQPDDRVALCVERSLEMMV 538
Cdd:PRK07769   16 RFPPNTNLVRHVERWAKVRGDKLAYRFLDfsterdgvaRDLTWSQFGARNRAVGARLQQVT-KPGDRVAILAPQNLDYLI 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  539 GLLGILKAGAAYVPM-DPAYP--AERLAYILDDAAPVALLTQSAQ---VAQLNSTLPTVllDTP---AAAACPDT----- 604
Cdd:PRK07769   95 AFFGALYAGRIAVPLfDPAEPghVGRLHAVLDDCTPSAILTTTDSaegVRKFFRARPAK--ERPrviAVDAVPDEvgatw 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  605 NPVVqgLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSR----------IALnASIVFDASVKNWI 674
Cdd:PRK07769  173 VPPE--ANEDTIAYLQYTSGSTRIPAGVQITHLNLPTNVLQVIDALEGQEGDRgvswlpffhdMGL-ITVLLPALLGHYI 249

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  675 QLLSGHTLVLVP----DALRADAHQLWRYFARHAVDLFDCT-----PVQLQWLLD----AGL--GSDPAyQPAQVLIGGE 739
Cdd:PRK07769  250 TFMSPAAFVRRPgrwiRELARKPGGTGGTFSAAPNFAFEHAaarglPKDGEPPLDlsnvKGLlnGSEPV-SPASMRKFNE 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  740 AISPavwsrlQSLSDTRFINVYGPTECT-------VDATACV--VDRTQ-----------------PLPTIGKPLANTRL 793
Cdd:PRK07769  329 AFAP------YGLPPTAIKPSYGMAEATlfvsttpMDEEPTViyVDRDElnagrfvevpadapnavAQVSAGKVGVSEWA 402

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  794 YILDA-QDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERF-------IPDPFS--ADPAARIYKTGDLARWLpDGNIDY 863
Cdd:PRK07769  403 VIVDPeTASELPDGQIGEIWLHGNNIGTGYWGKPEETAATFqnilksrLSESHAegAPDDALWVRTGDYGVYF-DGELYI 481


                  ...
gi 641744967  864 LGR 866
Cdd:PRK07769  482 TGR 484
LCL_NRPS-like cd19540
LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; ...
 2163-2463 3.97e-15

LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380463 [Multi-domain]  Cd Length: 433  Bit Score: 80.93  E-value: 3.97e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2163 PLAPLQEGILFHHLLQEQGDTYLLRsmVAFTHRERLD--AFLSALQQVIDRHDILRTaVCWQDLSQPVQVVW--RQAILP 2238
Cdd:cd19540     3 PLSFAQQRLWFLNRLDGPSAAYNIP--LALRLTGALDvdALRAALADVVARHESLRT-VFPEDDGGPYQVVLpaAEARPD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2239 INHfEPTSPEDVLAQLQAHTeprTRRIDLSQAPLFRAD-IAHDPlqNEWLLALSFHHLISDHMTLALIVGEI------RL 2311
Cdd:cd19540    80 LTV-VDVTEDELAARLAEAA---RRGFDLTAELPLRARlFRLGP--DEHVLVLVVHHIAADGWSMAPLARDLatayaaRR 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2312 llqhqADALP--TPLP--YRNFiaqTL---------SVPNS---AHEAYFRDKLADVDEPTA-------PfgllNVQGSG 2368
Cdd:cd19540   154 -----AGRAPdwAPLPvqYADY---ALwqrellgdeDDPDSlaaRQLAYWRETLAGLPEELElptdrprP----AVASYR 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2369 GDIHEARLvlDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLR 2448
Cdd:cd19540   222 GGTVEFTI--DAELHARLAALAREHGATLFMVLHAALAVLLSRLGAGDDIPIGTPVAGR--GDEALDDLVGMFVNTLVLR 297

                         330
                  ....*....|....*.
gi 641744967 2449 ISLA-DRGAAEVVERT 2463
Cdd:cd19540   298 TDVSgDPTFAELLARV 313
PRK08180 PRK08180
feruloyl-CoA synthase; Reviewed
 1538-1956 6.41e-15

feruloyl-CoA synthase; Reviewed


Pssm-ID: 236175 [Multi-domain]  Cd Length: 614  Bit Score: 81.08  E-value: 6.41e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1538 AARTPDTTAVLFED-----QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGY 1612
Cdd:PRK08180   49 AQEAPDRVFLAERGadggwRRLTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEHALLALAAMYAGVPYAPVSPAY 128

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1613 PA-----ERLAYMLDDASPVALLTQANQR------ALLTGDVPRILL-------DTADFSHLSEDNPHvPGLDAHH---- 1670
Cdd:PRK08180  129 SLvsqdfGKLRHVLELLTPGLVFADDGAAfaralaAVVPADVEVVAVrgavpgrAATPFAALLATPPT-AAVDAAHaavg 207

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1671 ---LAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYrltpddRVLQKTPfsfDVSVWEFFWPLLYGARLVMARPDGHK 1747
Cdd:PRK08180  208 pdtIAKFLFTSGSTGLPKAVINTHRMLCANQQMLAQTF------PFLAEEP---PVLVDWLPWNHTFGGNHNLGIVLYNG 278

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1748 DAAYL------AQLIERT------GITTLHF-VPSMLQQFVQWADADcacdslrrvicsgealpAELQQRFFAR----FN 1810
Cdd:PRK08180  279 GTLYIddgkptPGGFDETlrnlreISPTVYFnVPKGWEMLVPALERD-----------------AALRRRFFSRlkllFY 341

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1811 A----------QLHNL--------------YGPTEAAIDVTFwaCQPDDHRSFVpIGRPIANTQLYIldtlgqpVPLGVA 1866
Cdd:PRK08180  342 AgaalsqdvwdRLDRVaeatcgerirmmtgLGMTETAPSATF--TTGPLSRAGN-IGLPAPGCEVKL-------VPVGGK 411

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1867 GELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWL----PDGSLEYLGR--NDFqvKL-RGFRIELG 1939
Cdd:PRK08180  412 LEVRVKGPNVTPGYWRAPELTAEAFDEEGY--------YRSGDAVRFVdpadPERGLMFDGRiaEDF--KLsSGTWVSVG 481

                         490
                  ....*....|....*....
gi 641744967 1940 EIEARL-MQC-PGVQEAVV 1956
Cdd:PRK08180  482 PLRARAvSAGaPLVQDVVI 500
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
 1525-2021 6.41e-15

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 80.79  E-value: 6.41e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1525 PHDALIHQLVEDQAARTPDTTAVL--FEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAG 1602
Cdd:PLN02330   25 PDKLTLPDFVLQDAELYADKVAFVeaVTGKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAG 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1603 AAYVPLDPGYPAERLAYMLDDASPVALLTQANQRALLTG-DVPRI---------------LLDTADFSHLSEDNPHVPGL 1666
Cdd:PLN02330  105 GVFSGANPTALESEIKKQAEAAGAKLIVTNDTNYGKVKGlGLPVIvlgeekiegavnwkeLLEAADRAGDTSDNEEILQT 184

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1667 DahhLAYVIYTSGSTGKPKGVMNSHRALCNRLVwmQNTYRLTPDD----RVLQKTPFSFDVSVWEFFWPLLY--GARLVM 1740
Cdd:PLN02330  185 D---LCALPFSSGTTGISKGVMLTHRNLVANLC--SSLFSVGPEMigqvVTLGLIPFFHIYGITGICCATLRnkGKVVVM 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1741 ARPDGHkdaAYLAQLIERTgITTLHFVPSMLQQFVQ---WADADCACDSLRRVICSGEALPAELQQRFFARF-NAQLHNL 1816
Cdd:PLN02330  260 SRFELR---TFLNALITQE-VSFAPIVPPIILNLVKnpiVEEFDLSKLKLQAIMTAAAPLAPELLTAFEAKFpGVQVQEA 335

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1817 YGPTEAAIdVTFWACQPDDHRSFV---PIGRPIANTQLYILD-TLGQPVPLGVAGELHIGGVGVARGYLNRPDLTAERFI 1892
Cdd:PLN02330  336 YGLTEHSC-ITLTHGDPEKGHGIAkknSVGFILPNLEVKFIDpDTGRSLPKNTPGELCVRSQCVMQGYYNNKEETDRTID 414

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1893 PDPFINqpgarlykTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYL 1972
Cdd:PLN02330  415 EDGWLH--------TGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACV 486

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967 1973 CPQPGVTPDPADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:PLN02330  487 VINPKAKESEEDILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLL 535
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
 2632-3031 7.14e-15

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 80.59  E-value: 7.14e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALI--- 2708
Cdd:cd05932     7 FTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFvgk 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2709 -----SQSAHLGimnGSLPVILLDDGETRPFDNEPDTpLDARKQGL--TPRH----LAYVIYTSGSTGKPKGVMVEHANM 2777
Cdd:cd05932    87 lddwkAMAPGVP---EGLISISLPPPSAANCQYQWDD-LIAQHPPLeeRPTRfpeqLATLIYTSGTTGQPKGVMLTFGSF 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2778 VNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFL-PLLNGARLILATQAQAADAQqlamlIERHAVSFMQATPSTWRM 2856
Cdd:cd05932   163 AWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGgSLYGGVLVAFAESLDTFVED-----VQRARPTLFFSVPRLWTK 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2857 -------------------------LVE---LRDFALPPGFKALCGGEALPENLATALLQKVTTLWNLYGPTETTIWSTL 2908
Cdd:cd05932   238 fqqgvqdkipqqklnlllkipvvnsLVKrkvLKGLGLDQCRLAGCGSAPVPPALLEWYRSLGLNILEAYGMTENFAYSHL 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2909 N-GLTTPTPYIGHPIANTQIYILDaqgrvvplgvAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLG 2987
Cdd:cd05932   318 NyPGRDKIGTVGNAGPGVEVRISE----------DGEILVRSPALMMGYYKDPEATAEAFTADGF--------LRTGDKG 379

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*
gi 641744967 2988 RWLPDGTLEYLGRNDFQVKV-RGFRIELGEIETRLArchgVHDAV 3031
Cdd:cd05932   380 ELDADGNLTITGRVKDIFKTsKGKYVAPAPIENKLA----EHDRV 420
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
 485-963 7.31e-15

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 80.65  E-value: 7.31e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  485 QTPEAIAvlFEDQH----LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAE 560
Cdd:cd17642    29 SVPGTIA--FTDAHtgvnYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNER 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  561 RLAYILDDAAPVALLTQSA---QVAQLNSTLPTV----LLDTP---AAAACPD---TNPVVQGLHAAH-----------L 616
Cdd:cd17642   107 ELDHSLNISKPTIVFCSKKglqKVLNVQKKLKIIktiiILDSKedyKGYQCLYtfiTQNLPPGFNEYDfkppsfdrdeqV 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  617 AYVIYTSGSTGRPKGVMVAHRNV---------------INLATGLHTLLALDHP-------------SRIALNASivFDA 668
Cdd:cd17642   187 ALIMNSSGSTGLPKGVQLTHKNIvarfshardpifgnqIIPDTAILTVIPFHHGfgmfttlgylicgFRVVLMYK--FEE 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  669 svKNWIQLLSGH---TLVLVPdalradahQLWRYFARHA-VDLFDCTPVQlqwlldaglgsdpayqpaQVLIGGEAISPA 744
Cdd:cd17642   265 --ELFLRSLQDYkvqSALLVP--------TLFAFFAKSTlVDKYDLSNLH------------------EIASGGAPLSKE 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  745 VWSRLQSLSDTRFINV-YGPTECTvdATACVVDRTQPLP-TIGKPLANTRLYILDAqDQPVPIGV--TGELHIGGAGVAR 820
Cdd:cd17642   317 VGEAVAKRFKLPGIRQgYGLTETT--SAILITPEGDDKPgAVGKVVPFFYAKVVDL-DTGKTLGPneRGELCVKGPMIMK 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  821 GYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIA 900
Cdd:cd17642   394 GYVNNPEATKALIDKDGW--------LHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAG 465

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967  901 REDspgdtrlvaylcarPDAELHPAAL----------RQQLAASLADYMIPS-----AFVTLDALPLTPNGKLDRKAL 963
Cdd:cd17642   466 IPD--------------EDAGELPAAVvvleagktmtEKEVMDYVASQVSTAkrlrgGVKFVDEVPKGLTGKIDRRKI 529
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
 2621-3099 7.41e-15

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 80.84  E-value: 7.41e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2621 DAIAVVFGEASLSYDELNRRANRLAHHLISFGvRPDER--VAICVERGLDMVVGLLGILKAGGAYVPLDPTypvERLRYM 2698
Cdd:PRK13388   16 DTIAVRYGDRTWTWREVLAEAAARAAALIALA-DPDRPlhVGVLLGNTPEMLFWLAAAALGGYVLVGLNTT---RRGAAL 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPV---ALISQSAHLGIMNG----SLPVILLDDGETRPFDNEPDTPLDARKqgLTPRHLAYVIYTSGSTGKPKGVM 2771
Cdd:PRK13388   92 AADIRRAdcqLLVTDAEHRPLLDGldlpGVRVLDVDTPAYAELVAAAGALTPHRE--VDAMDPFMLIFTSGTTGAPKAVR 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2772 VEHANMVNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLPLL-NGARLILATQAQAADAQQLamlIERHAVSFMQ-- 2848
Cdd:PRK13388  170 CSHGRLAFAGRALTERFGLTRDDVCYVSMPLFHSNAVMAGWAPAVaSGAAVALPAKFSASGFLDD---VRRYGATYFNyv 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2849 --------ATPStwrmLVELRDFALPPGFkalcGGEALPENLATALLQKVTTLWNLYGPTETTIWSTLNGlTTPTPYIGH 2920
Cdd:PRK13388  247 gkplayilATPE----RPDDADNPLRVAF----GNEASPRDIAEFSRRFGCQVEDGYGSSEGAVIVVREP-GTPPGSIGR 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2921 PIANTQIY-----------ILDAQGRVV-PLGVAGEI-HIAGAGVVRGYLGRPDLTAERFitdpfsgapeaR--MYKTGD 2985
Cdd:PRK13388  318 GAPGVAIYnpetltecavaRFDAHGALLnADEAIGELvNTAGAGFFEGYYNNPEATAERM-----------RhgMYWSGD 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2986 LGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDS-PGDKRLVAYLLAQPDTvLEPADLRQR 3064
Cdd:PRK13388  387 LAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDErVGDQVMAALVLRDGAT-FDPDAFAAF 465

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 641744967 3065 LS--EGVAEYMIPSAFVTLDAFPLTPNGKLDRKALPA 3099
Cdd:PRK13388  466 LAaqPDLGTKAWPRYVRIAADLPSTATNKVLKRELIA 502
PRK09029 PRK09029
O-succinylbenzoic acid--CoA ligase; Provisional
 2620-3097 9.93e-15

O-succinylbenzoic acid--CoA ligase; Provisional


Pssm-ID: 236363 [Multi-domain]  Cd Length: 458  Bit Score: 79.92  E-value: 9.93e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:PRK09029   17 PQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQPLLEELL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAkpvalisQSAHLGIMNGSLPVILLDDGETRPFDNEPDTPLDARKqgltprhLAYVIYTSGSTGKPKGVmVEHANmvN 2779
Cdd:PRK09029   97 PSL-------TLDFALVLEGENTFSALTSLHLQLVEGAHAVAWQPQR-------LATMTLTSGSTGLPKAA-VHTAQ--A 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2780 FLCSMRkepGI-------AQEDVLLgvtSLS-FDISILEIFLP-LLNGARLILatqaqAADAQQLAMLierHAVSFMQAT 2850
Cdd:PRK09029  160 HLASAE---GVlslmpftAQDSWLL---SLPlFHVSGQGIVWRwLYAGATLVV-----RDKQPLEQAL---AGCTHASLV 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2851 PST-WRMLVELrdfALPPGFKA-LCGGEALPENLATALLQKVTTLWNLYGPTETTiwSTL-----NGLttptPYIGHPIA 2923
Cdd:PRK09029  226 PTQlWRLLDNR---SEPLSLKAvLLGGAAIPVELTEQAEQQGIRCWCGYGLTEMA--STVcakraDGL----AGVGSPLP 296

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2924 NTQIYIldaqgrvvplgVAGEIHIAGAGVVRGYLGRPDLTAerfITDpfsgapEARMYKTGDLGRWLpDGTLEYLGRNDF 3003
Cdd:PRK09029  297 GREVKL-----------VDGEIWLRGASLALGYWRQGQLVP---LVN------DEGWFATRDRGEWQ-NGELTILGRLDN 355

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3004 QVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLlaQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDA 3083
Cdd:PRK09029  356 LFFSGGEGIQPEEIERVINQHPLVQQVFVVPVADAEFGQRPVAVV--ESDSEAAVVNLAEWLQDKLARFQQPVAYYLLPP 433

                         490
                  ....*....|....
gi 641744967 3084 FPLTPNGKLDRKAL 3097
Cdd:PRK09029  434 ELKNGGIKISRQAL 447
PRK07867 PRK07867
acyl-CoA synthetase; Validated
 2621-3099 1.02e-14

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 80.11  E-value: 1.02e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2621 DAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDER-VAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:PRK07867   18 DDRGLYFEDSFTSWREHIRGSAARAAALRARLDPTRPPhVGVLLDNTPEFSLLLGAAALSGIVPVGLNPTRRGAALARDI 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALISQSAHLGIMNGSLPVILLDDGETRPFDNEPDTPLDA--RKQGLTPRHLAYVIYTSGSTGKPKGVMVEHANM 2777
Cdd:PRK07867   98 AHADCQLVLTESAHAELLDGLDPGVRVINVDSPAWADELAAHRDAepPFRVADPDDLFMLIFTSGTSGDPKAVRCTHRKV 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2778 VNFLCSMRKEPGIAQEDVLLGVTSLSFDISILEIFLP-LLNGARLILATQAQAADAQQLamlIERH----------AVSF 2846
Cdd:PRK07867  178 ASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVaLAAGASIALRRKFSASGFLPD---VRRYgatyanyvgkPLSY 254

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2847 MQATPstwrmlvELRDFALPPgFKALCGGEALPENLATALLQKVTTLWNLYGPTETTIwSTLNGLTTPTPYIGHPIANTQ 2926
Cdd:PRK07867  255 VLATP-------ERPDDADNP-LRIVYGNEGAPGDIARFARRFGCVVVDGFGSTEGGV-AITRTPDTPPGALGPLPPGVA 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2927 IY-----------ILDAQGRVVPLGVAGE-IHIAGAGVVRGYLGRPDLTAERFitdpfsgapEARMYKTGDLGRWLPDGT 2994
Cdd:PRK07867  326 IVdpdtgtecppaEDADGRLLNADEAIGElVNTAGPGGFEGYYNDPEADAERM---------RGGVYWSGDLAYRDADGY 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2995 LEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSE--GVAEY 3072
Cdd:PRK07867  397 AYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKFDPDAFAEFLAAqpDLGPK 476

                         490       500
                  ....*....|....*....|....*..
gi 641744967 3073 MIPSAFVTLDAFPLTPNGKLDRKALPA 3099
Cdd:PRK07867  477 QWPSYVRVCAELPRTATFKVLKRQLSA 503
LCL_NRPS cd19538
LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; ...
 2163-2449 1.05e-14

LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380461 [Multi-domain]  Cd Length: 432  Bit Score: 79.62  E-value: 1.05e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2163 PLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTaVCWQDLSQPVQVVW--RQAILPIN 2240
Cdd:cd19538     3 PLSFAQRRLWFLHQLEGPSATYNIPLVIKLKGKLDVQALQQALYDVVERHESLRT-VFPEEDGVPYQLILeeDEATPKLE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2241 HFEpTSPEDVLAQLQAHTEprtRRIDLSQAPLFRADIaHDPLQNEWLLALSFHHLISDHMTLALIVGEI-----RLLLQH 2315
Cdd:cd19538    82 IKE-VDEEELESEINEAVR---YPFDLSEEPPFRATL-FELGENEHVLLLLLHHIAADGWSLAPLTRDLskayrARCKGE 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2316 QADALPTPLPYRNFIA------QTLSVPNSAHE---AYFRDKLADVDE----PTAPFGLLNVQGSGGDIheaRLVLDATL 2382
Cdd:cd19538   157 APELAPLPVQYADYALwqqellGDESDPDSLIArqlAYWKKQLAGLPDeielPTDYPRPAESSYEGGTL---TFEIDSEL 233

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 2383 ASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRI 2449
Cdd:cd19538   234 HQQLLQLAKDNNVTLFMVLQAGFAALLTRLGAGTDIPIGSPVAGR--NDDSLEDLVGFFVNTLVLRT 298
PRK07008 PRK07008
long-chain-fatty-acid--CoA ligase; Validated
 2633-3092 1.06e-14

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235908 [Multi-domain]  Cd Length: 539  Bit Score: 80.14  E-value: 1.06e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2633 SYDELNRRANRLAHHLISFGVRPDERVAICVERG---LDMVVGLLGilkAGGAYVPLDPTYPVERLRYMLDDAKPVALIS 2709
Cdd:PRK07008   41 TYRDCERRAKQLAQALAALGVEPGDRVGTLAWNGyrhLEAYYGVSG---SGAVCHTINPRLFPEQIAYIVNHAEDRYVLF 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2710 QSAHLGIMNGSLP-------VILLDDGETRPFDNEP----DTPLDARKQG-----LTPRHLAYVIYTSGSTGKPKGVMVE 2773
Cdd:PRK07008  118 DLTFLPLVDALAPqcpnvkgWVAMTDAAHLPAGSTPllcyETLVGAQDGDydwprFDENQASSLCYTSGTTGNPKGALYS 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2774 HANMV--NFLCSMRKEPGIAQEDVLLGVTSLsFDISILEI-FLPLLNGARLILATQAQAADAQQLamLIERHAVSFMQAT 2850
Cdd:PRK07008  198 HRSTVlhAYGAALPDAMGLSARDAVLPVVPM-FHVNAWGLpYSAPLTGAKLVLPGPDLDGKSLYE--LIEAERVTFSAGV 274

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2851 PSTWRML---VELRDFALPPGFKALCGGEALPENLATALLQ----KVTTLWnlyGPTETTIWSTLNGLTTPTPYI----- 2918
Cdd:PRK07008  275 PTVWLGLlnhMREAGLRFSTLRRTVIGGSACPPAMIRTFEDeygvEVIHAW---GMTEMSPLGTLCKLKWKHSQLpldeq 351

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2919 -------GHPIANTQIYILDAQGRVVPL-GVA-GEIHIAGAGVVRGYLGRPDltaerfitDPFSgapeARMYKTGDLGRW 2989
Cdd:PRK07008  352 rkllekqGRVIYGVDMKIVGDDGRELPWdGKAfGDLQVRGPWVIDRYFRGDA--------SPLV----DGWFPTGDVATI 419

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2990 LPDGTLEYLGRNDFQVKVRGFRIELGEIETrLARCH-GVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEG 3068
Cdd:PRK07008  420 DADGFMQITDRSKDVIKSGGEWISSIDIEN-VAVAHpAVAEAACIACAHPKWDERPLLVVVKRPGAEVTREELLAFYEGK 498

                         490       500
                  ....*....|....*....|....
gi 641744967 3069 VAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:PRK07008  499 VAKWWIPDDVVFVDAIPHTATGKL 522
LC_FACS_bac1 cd17641
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ...
 497-918 1.35e-14

bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341296 [Multi-domain]  Cd Length: 569  Bit Score: 80.16  E-value: 1.35e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  497 QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLT 576
Cdd:cd17641    10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  577 ----QSAQVAQLNSTLPTVL------------LDTPAAAACPDTNPVVQGLHAAH---------------LAYVIYTSGS 625
Cdd:cd17641    90 edeeQVDKLLEIADRIPSVRyviycdprgmrkYDDPRLISFEDVVALGRALDRRDpglyerevaagkgedVAVLCTTSGT 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  626 TGRPKGVMVAHRNVINLATGLhtlLALDHPSRIALNASIVFDAsvknWI--QLLS------------------------- 678
Cdd:cd17641   170 TGKPKLAMLSHGNFLGHCAAY---LAADPLGPGDEYVSVLPLP----WIgeQMYSvgqalvcgfivnfpeepetmmedlr 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  679 --GHTLVLVP-----------DALRADAHQLWRYFARHAVDL--------FDCTPVQLQWLLDAGLGSDPAYQPAQVLIG 737
Cdd:cd17641   243 eiGPTFVLLPprvwegiaadvRARMMDATPFKRFMFELGMKLglraldrgKRGRPVSLWLRLASWLADALLFRPLRDRLG 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  738 ----------GEAISPAVWSRLQSLS-DTRfiNVYGPTECtvdATACVVDRTQPLP--TIGKPLANTRlyildaqdqpVP 804
Cdd:cd17641   323 fsrlrsaatgGAALGPDTFRFFHAIGvPLK--QLYGQTEL---AGAYTVHRDGDVDpdTVGVPFPGTE----------VR 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  805 IGVTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGR-NDFQIKVRGFRIEAGEI 883
Cdd:cd17641   388 IDEVGEILVRSPGVFVGYYKNPEATAEDFDEDGW--------LHTGDAGYFKENGHLVVIDRaKDVGTTSDGTRFSPQFI 459

                         490       500       510
                  ....*....|....*....|....*....|....*
gi 641744967  884 ESRLLRCPGVQDAVVIARedspGDTRLVAYLCARP 918
Cdd:cd17641   460 ENKLKFSPYIAEAVVLGA----GRPYLTAFICIDY 490
A_NRPS_MycA_like cd05908
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ...
 487-884 1.53e-14

The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341234 [Multi-domain]  Cd Length: 499  Bit Score: 79.45  E-value: 1.53e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  487 PEAIAVLFEDQ---HLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGaaYVPMdpaypaerla 563
Cdd:cd05908     1 PEGIIFILGDKkekFVSYRHLREEALGYLGALQELGIKPGQEVVFQITHNNKFLYLFWACLLGG--MIAV---------- 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  564 yilddaaPVALLTQSAQVAQLNSTLPTvlLDTPAAAacpdTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVInla 643
Cdd:cd05908    69 -------PVSIGSNEEHKLKLNKVWNT--LKNPYLI----TEEEVLCELADELAFIQFSSGSTGDPKGVMLTHENLV--- 132

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  644 tglHTLLALDHPSRIALNASIVFDASVKNWIQLLSGHTL--------VLVPDALRADAHQLWRYFA-RHAVDLFDCTPVQ 714
Cdd:cd05908   133 ---HNMFAILNSTEWKTKDRILSWMPLTHDMGLIAFHLApliagmnqYLMPTRLFIRRPILWLKKAsEHKATIVSSPNFG 209

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  715 LQWLLDAgLGSDPAYQ-----PAQVLIGGEAISPAVWSRLQS------LSDTRFINVYGPTECTVDAT------------ 771
Cdd:cd05908   210 YKYFLKT-LKPEKANDwdlssIRMILNGAEPIDYELCHEFLDhmskygLKRNAILPVYGLAEASVGASlpkaqspfktit 288

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  772 -----------ACVVDRTQP----LPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAERFIPD 836
Cdd:cd05908   289 lgrrhvthgepEPEVDKKDSecltFVEVGKPIDETDIRICDEDNKILPDGYIGHIQIRGKNVTPGYYNNPEATAKVFTDD 368

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 641744967  837 PFsadpaariYKTGDLArWLPDGNIDYLGRNDFQIKVRGFRIEAGEIE 884
Cdd:cd05908   369 GW--------LKTGDLG-FIRNGRLVITGREKDIIFVNGQNVYPHDIE 407
PRK00174 PRK00174
acetyl-CoA synthetase; Provisional
 490-963 3.51e-14

acetyl-CoA synthetase; Provisional


Pssm-ID: 234677 [Multi-domain]  Cd Length: 637  Bit Score: 78.64  E-value: 3.51e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  490 IAVLFE------DQHLTYRELNRRANQLAHHLIALGVQPDDRVALcverslemmvgllgilkagaaYVPMDP--AY---- 557
Cdd:PRK00174   84 VAIIWEgddpgdSRKITYRELHREVCRFANALKSLGVKKGDRVAI---------------------YMPMIPeaAVamla 142

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  558 ---------------PAERLAYILDDAAPVALLTQSAQV---------------AQLNSTLPTVL--------------- 592
Cdd:PRK00174  143 carigavhsvvfggfSAEALADRIIDAGAKLVITADEGVrggkpiplkanvdeaLANCPSVEKVIvvrrtggdvdwvegr 222

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  593 ------LDTPAAAACPdtnPVVqgLHAAHLAYVIYTSGSTGRPKGVMvaHrnvinlATGLHTLLAldhpsriALNASIVF 666
Cdd:PRK00174  223 dlwwheLVAGASDECE---PEP--MDAEDPLFILYTSGSTGKPKGVL--H------TTGGYLVYA-------AMTMKYVF 282

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  667 D----------ASVkNWIqllSGHT-LVLVPDALRA------------DAHQLWRYFARHAVDLFDCTPVQLQWLLdaGL 723
Cdd:PRK00174  283 DykdgdvywctADV-GWV---TGHSyIVYGPLANGAttlmfegvpnypDPGRFWEVIDKHKVTIFYTAPTAIRALM--KE 356

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  724 GSDPayqPAQV------LIG--GEAISPAVWsrlqslsdTRFINVYGPTECTVdatacvVD---RTQ-------PLP--T 783
Cdd:PRK00174  357 GDEH---PKKYdlsslrLLGsvGEPINPEAW--------EWYYKVVGGERCPI------VDtwwQTEtggimitPLPgaT 419

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  784 IGKPLANTR-LY-----ILDAQDQPVPIGVTGELHIGGA--GVARGYLHRPdltaERFIPDPFSADPAarIYKTGDLARW 855
Cdd:PRK00174  420 PLKPGSATRpLPgiqpaVVDEEGNPLEGGEGGNLVIKDPwpGMMRTIYGDH----ERFVKTYFSTFKG--MYFTGDGARR 493

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  856 LPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIARedsPGDTR---LVAY--LCA--RPDAELHpAALR 928
Cdd:PRK00174  494 DEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGR---PDDIKgqgIYAFvtLKGgeEPSDELR-KELR 569

                         570       580       590       600
                  ....*....|....*....|....*....|....*....|.
gi 641744967  929 QQLA------ASLADYMipsaFVtlDALPLTPNGKLDRKAL 963
Cdd:PRK00174  570 NWVRkeigpiAKPDVIQ----FA--PGLPKTRSGKIMRRIL 604
PLN02387 PLN02387
long-chain-fatty-acid-CoA ligase family protein
 494-900 3.94e-14

long-chain-fatty-acid-CoA ligase family protein


Pssm-ID: 215217 [Multi-domain]  Cd Length: 696  Bit Score: 78.62  E-value: 3.94e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  494 FEDQHL------TYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILD 567
Cdd:PLN02387   96 FEKLHLgeyewiTYGQVFERVCNFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLN 175

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  568 DAAPVALLTQSAQ---VAQLNSTLPTV-----------LLDTPAAAACP-------------DTNPVVQGL-HAAHLAYV 619
Cdd:PLN02387  176 ETEVTTVICDSKQlkkLIDISSQLETVkrviymddegvDSDSSLSGSSNwtvssfseveklgKENPVDPDLpSPNDIAVI 255

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  620 IYTSGSTGRPKGVMVAHRNVINLATGLHTL-------------LALDHPSRIAlnASIVFdASVKNWIQLLSGHTL---- 682
Cdd:PLN02387  256 MYTSGSTGLPKGVMMTHGNIVATVAGVMTVvpklgkndvylayLPLAHILELA--AESVM-AAVGAAIGYGSPLTLtdts 332

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  683 ------------VLVPDALRADAHQLWRYF--ARHAVD--------LFD------CTPVQLQWL----LDAGLGSDPAYQ 730
Cdd:PLN02387  333 nkikkgtkgdasALKPTLMTAVPAILDRVRdgVRKKVDakgglakkLFDiaykrrLAAIEGSWFgawgLEKLLWDALVFK 412

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  731 PAQVLIGGeaispavwsRLQSL--------SDT-RFINV---------YGPTECTVDATACVVDRTQpLPTIGKPLANTR 792
Cdd:PLN02387  413 KIRAVLGG---------RIRFMlsggaplsGDTqRFINIclgapigqgYGLTETCAGATFSEWDDTS-VGRVGPPLPCCY 482

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  793 LYILD-------AQDQPVPigvTGELHIGGAGVARGYLHRPDLTAERFIPDpfsaDPAARIYKTGDLARWLPDGNIDYLG 865
Cdd:PLN02387  483 VKLVSweeggylISDKPMP---RGEIVIGGPSVTLGYFKNQEKTDEVYKVD----ERGMRWFYTGDIGQFHPDGCLEIID 555

                         490       500       510
                  ....*....|....*....|....*....|....*.
gi 641744967  866 RNDFQIKVR-GFRIEAGEIESRLLRCPGVQDAVVIA 900
Cdd:PLN02387  556 RKKDIVKLQhGEYVSLGKVEAALSVSPYVDNIMVHA 591
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
1843-2021 6.84e-14

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 76.24  E-value: 6.84e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1843 GRPIANTQLYILDtlgqpvplgvaGELHIGGVGVARGYLNRPDltaerfiPDPFInQPGarLYKTGDLARwLPDGSLEYL 1922
Cdd:PRK07824  195 GVPLDGVRVRVED-----------GRIALGGPTLAKGYRNPVD-------PDPFA-EPG--WFRTDDLGA-LDDGVLTVL 252

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1923 GRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPADLRQQLGQHLAEYMVPGAF 2002
Cdd:PRK07824  253 GRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDGGPAPTLEALRAHVARTLDRTAAPREL 332

                         170
                  ....*....|....*....
gi 641744967 2003 VTLDAFPLTPNGKLDRKAL 2021
Cdd:PRK07824  333 HVVDELPRRGIGKVDRRAL 351
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
 500-963 1.08e-13

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 76.74  E-value: 1.08e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  500 TYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:cd05928    43 SFRELGSLSRKAANVLSgACGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSD 122

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 AQVAQLNS------TLPTVLLDTPAA------------AACPDTNPVVQGLHAAHLAYviYTSGSTGRPKgvMVAHRNV- 639
Cdd:cd05928   123 ELAPEVDSvasecpSLKTKLLVSEKSrdgwlnfkellnEASTEHHCVETGSQEPMAIY--FTSGTTGSPK--MAEHSHSs 198

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  640 --INLATGLHTLLALDhPSRIALNAS----IVFDAS--VKNWIQllsGHTlVLVPDALRADAHQLWRYFARHAVDLFDCT 711
Cdd:cd05928   199 lgLGLKVNGRYWLDLT-ASDIMWNTSdtgwIKSAWSslFEPWIQ---GAC-VFVHHLPRFDPLVILKTLSSYPITTFCGA 273

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  712 PVQLQWLLDAGLGSdpaYQ-PA--QVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVdatACVVDRTQPLP--TIGK 786
Cdd:cd05928   274 PTVYRMLVQQDLSS---YKfPSlqHCVTGGEPLNPEVLEKWKAQTGLDIYEGYGQTETGL---ICANFKGMKIKpgSMGK 347

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  787 PLANTRLYILDAQDQPVPIGVTGELHI-----GGAGVARGYLHRPDLTAERFIPDpfsadpaarIYKTGDLARWLPDGNI 861
Cdd:cd05928   348 ASPPYDVQIIDDNGNVLPPGTEGDIGIrvkpiRPFGLFSGYVDNPEKTAATIRGD---------FYLTGDRGIMDEDGYF 418

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  862 DYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAredSPGDTR---LVAYLCARPDAELH-----PAALRQQLAA 933
Cdd:cd05928   419 WFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVS---SPDPIRgevVKAFVVLAPQFLSHdpeqlTKELQQHVKS 495

                         490       500       510
                  ....*....|....*....|....*....|
gi 641744967  934 SLADYMIPSAFVTLDALPLTPNGKLDRKAL 963
Cdd:cd05928   496 VTAPYKYPRKVEFVQELPKTVTGKIQRNEL 525
C_PKS-NRPS cd20483
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 30-368 1.28e-13

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHXXXD motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380471 [Multi-domain]  Cd Length: 430  Bit Score: 76.14  E-value: 1.28e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEkgDTYLLNSLLAF--DSQTRLDAFLDVLQQVIARHDILRTAICwQGLHQPVQVVWRQAPLTVN 107
Cdd:cd20483     3 PMSTFQRRLWFLHNFLE--DKTFLNLLLVChiKGKPDVNLLQKALSELVRRHEVLRTAYF-EGDDFGEQQVLDDPSFHLI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  108 TLTTTSSDTVPAQL-RAATDPSNHRLNLSNAPLLSATTAHDPVcGEWLLSLSIHHLISDHITQALIIDEI----RLLLED 182
Cdd:cd20483    80 VIDLSEAADPEAALdQLVRNLRRQELDIEEGEVIRGWLVKLPD-EEFALVLASHHIAWDRGSSKSIFEQFtalyDALRAG 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  183 RPEALPKPLPYrNFI-------AQILSVPLSEHEQYFRNRLADIDTPTA--PFDLVD---VQGNGEDITEArlSLDSSLA 250
Cdd:cd20483   159 RDLATVPPPPV-QYIdftlwhnALLQSPLVQPLLDFWKEKLEGIPDASKllPFAKAErppVKDYERSTVEA--TLDKELL 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  251 DALRRQARHLGISSsvlFHVAWAQVLALTS---GRDDVVFGSVLSGRlqGNLGADRVMGMFINTLPLRVSLR-ERSVHDV 326
Cdd:cd20483   236 ARMKRICAQHAVTP---FMFLLAAFRAFLYrytEDEDLTIGMVDGDR--PHPDFDDLVGFFVNMLPIRCRMDcDMSFDDL 310

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*
gi 641744967  327 VQATSHELMMLLAHEQAPLALAQQCSQVPPPL---PLFSTLFNYR 368
Cdd:cd20483   311 LESTKTTCLEAYEHSAVPFDYIVDALDVPRSTshfPIGQIAVNYQ 355
FCS cd05921
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ...
 1555-1994 2.54e-13

Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.


Pssm-ID: 341245 [Multi-domain]  Cd Length: 561  Bit Score: 75.93  E-value: 2.54e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1555 TYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPA-----ERLAYMLDDASPVAL 1629
Cdd:cd05921    27 TYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVSPAYSLmsqdlAKLKHLFELLKPGLV 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1630 LTQAN---QRAL---LTGDVPRI----LLDTADFSHLSEDNPHVPGLDAHHL---------AYVIYTSGSTGKPKGVMNS 1690
Cdd:cd05921   107 FAQDAapfARALaaiFPLGTPLVvsrnAVAGRGAISFAELAATPPTAAVDAAfaavgpdtvAKFLFTSGSTGLPKAVINT 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1691 HRALCNRLVWMQNTY-RLTPDDRV-LQKTPFSFDVSVWEFFWPLLY-GARLVM--ARPDGHKDAAYLAQLIERTgiTTLH 1765
Cdd:cd05921   187 QRMLCANQAMLEQTYpFFGEEPPVlVDWLPWNHTFGGNHNFNLVLYnGGTLYIddGKPMPGGFEETLRNLREIS--PTVY 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1766 F-VPSMLQQFVQWADADCAC-----DSLRRVICSGEALPAELQQRFFA----------RFNAQlhnlYGPTEAAIDVTFw 1829
Cdd:cd05921   265 FnVPAGWEMLVAALEKDEALrrrffKRLKLMFYAGAGLSQDVWDRLQAlavatvgeriPMMAG----LGATETAPTATF- 339

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1830 aCQPDDHRSFVpIGRPIANTQLYIldtlgqpVPLGVAGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGD 1909
Cdd:cd05921   340 -THWPTERSGL-IGLPAPGTELKL-------VPSGGKYEVRVKGPNVTPGYWRQPELTAQAFDEEGF--------YCLGD 402

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1910 LARWL----PDGSLEYLGR--NDFqvKLR-GFRIELGEIEARLM-QCPGVQEAVVVAREDSPGDTRLV---AYLCPQ--P 1976
Cdd:cd05921   403 AAKLAdpddPAKGLVFDGRvaEDF--KLAsGTWVSVGPLRARAVaACAPLVHDAVVAGEDRAEVGALVfpdLLACRRlvG 480

                         490       500
                  ....*....|....*....|....
gi 641744967 1977 GVTPDPAD------LRQQLGQHLA 1994
Cdd:cd05921   481 LQEASDAEvlrhakVRAAFRDRLA 504
PRK08308 PRK08308
acyl-CoA synthetase; Validated
 2633-3099 2.91e-13

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 74.69  E-value: 2.91e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2633 SYDELNRRANRLAHHLIsFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQSA 2712
Cdd:PRK08308   10 SKSDFDLRLQRYEEMEQ-FQEAAGNRFAVCLKDPFDIITLVFFLKEKGASVLPIHPDTPKEAAIRMAKRAGCHGLLYGES 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2713 HLGImngslpvillddGETRPFDNEPDTPLDarkqgltprhlayviYTSGSTGKPKGVM-----VE---HANMVNFLCSM 2784
Cdd:PRK08308   89 DFTK------------LEAVNYLAEEPSLLQ---------------YSSGTTGEPKLIRrswteIDreiEAYNEALNCEQ 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2785 RKEPGIA--------------------QEDVLLGVTSLSFDISIL-EIFLPLLNGARLILatqaqaadaqqlamlierHA 2843
Cdd:PRK08308  142 DETPIVAcpvthsyglicgvlaaltrgSKPVIITNKNPKFALNILrNTPQHILYAVPLML------------------HI 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2844 VSfmqatpstwRMLVELRDFalppgFKALCGGEALPENLATALLQKVTTLWNLYGPTETTIWSTLNGLTTPTPyIGHPIA 2923
Cdd:PRK08308  204 LG---------RLLPGTFQF-----HAVMTSGTPLPEAWFYKLRERTTYMMQQYGCSEAGCVSICPDMKSHLD-LGNPLP 268

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2924 NTQIYILDAQGRvvplgvAGEIhiagagVVRgylgrpdlTAERFItdpfsgapearmyKTGDLGRWLPDGTLEYLGRNDF 3003
Cdd:PRK08308  269 HVSVSAGSDENA------PEEI------VVK--------MGDKEI-------------FTKDLGYKSERGTLHFMGRMDD 315

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3004 QVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAqpDTVLEPADLRQRLSEGVAEYMIPSAFVTLDA 3083
Cdd:PRK08308  316 VINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVIS--HEEIDPVQLREWCIQHLAPYQVPHEIESVTE 393

                         490
                  ....*....|....*.
gi 641744967 3084 FPLTPNGKLDRKALPA 3099
Cdd:PRK08308  394 IPKNANGKVSRKLLEL 409
hsFATP4_like cd05939
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ...
 1551-2021 5.77e-13

Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341262 [Multi-domain]  Cd Length: 474  Bit Score: 74.38  E-value: 5.77e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1551 DQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMlddaspvalL 1630
Cdd:cd05939     1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHC---------I 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1631 TQANQRALLTGDVPRILLDTadfshlSEDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNSHralcNRLVWMQ----NTYR 1706
Cdd:cd05939    72 TVSKAKALIFNLLDPLLTQS------STEPPSQDDVNFRDKLFYIYTSGTTGLPKAAVIVH----SRYYRIAagayYAFG 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1707 LTPDDRVLQKTPFSFD----VSVWEffwPLLYGARLVMARpdghKDAA--YLAQLIeRTGITTLHFVPSMLQQFVqwADA 1780
Cdd:cd05939   142 MRPEDVVYDCLPLYHSaggiMGVGQ---ALLHGSTVVIRK----KFSAsnFWDDCV-KYNCTIVQYIGEICRYLL--AQP 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1781 DCACDSLRRV-ICSGEALPAELQQRFFARFN-AQLHNLYGPTE-----AAIDVTFWACqpddhrSFVPIG----RPIA-- 1847
Cdd:cd05939   212 PSEEEQKHNVrLAVGNGLRPQIWEQFVRRFGiPQIGEFYGATEgnsslVNIDNHVGAC------GFNSRIlpsvYPIRli 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1848 ----NTQLYILDTLGQPVPL--GVAGELhiggVGVAR---------GYLNRPDlTAERFIPDPFinQPGARLYKTGDLAR 1912
Cdd:cd05939   286 kvdeDTGELIRDSDGLCIPCqpGEPGLL----VGKIIqndplrrfdGYVNEGA-TNKKIARDVF--KKGDSAFLSGDVLV 358

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1913 WLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVV--VAREDSPGDTRLVAYLCPQPGVtpDPADLRQQLG 1990
Cdd:cd05939   359 MDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVygVEVPGVEGRAGMAAIVDPERKV--DLDRFSAVLA 436

                         490       500       510
                  ....*....|....*....|....*....|.
gi 641744967 1991 QHLAEYMVPGAFVTLDAFPLTPNGKLDRKAL 2021
Cdd:cd05939   437 KSLPPYARPQFIRLLPEVDKTGTFKLQKTDL 467
Cyc_NRPS cd19535
Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 2196-2492 6.58e-13

Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Cyc (heterocyclization) domains catalyze two separate reactions in the creation of heterocyclized peptide products in nonribosomal peptide synthesis: amide bond formation followed by intramolecular cyclodehydration between a Cys, Ser, or Thr side chain and a carbonyl carbon on the peptide backbone to form a thiazoline, oxazoline, or methyloxazoline ring. Cyc-domains are homologous to standard NRPS Condensation (C) domains. C-domains typically have a conserved HHxxxD motif at the active site; Cyc-domains have an alternative, conserved DxxxxD active site motif, mutation of the aspartate residues in this motif can abolish or diminish condensation activity. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and Cyc-domains. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380458 [Multi-domain]  Cd Length: 423  Bit Score: 73.68  E-value: 6.58e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2196 ERLDAflsALQQVIDRHDILRTAVcwqdLSQPVQVVWRQ---AILPINHFEPTSPEDVLAQLQA------HteprtRRID 2266
Cdd:cd19535    40 DRLER---AWNKLIARHPMLRAVF----LDDGTQQILPEvpwYGITVHDLRGLSEEEAEAALEElrerlsH-----RVLD 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2267 LSQAPLFRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADALPtPLPY--RNFIAQTLSVPNSAHE-- 2342
Cdd:cd19535   108 VERGPLFDIRLSLLP-EGRTRLHLSIDLLVADALSLQILLRELAALYEDPGEPLP-PLELsfRDYLLAEQALRETAYEra 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2343 -AYFRDKLADVdePTAPfGL-LNVQGSggDIHEARLV-----LDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGR 2415
Cdd:cd19535   186 rAYWQERLPTL--PPAP-QLpLAKDPE--EIKEPRFTrrehrLSAEQWQRLKERARQHGVTPSMVLLTAYAEVLARWSGQ 260

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2416 DDVVFGSVLLGRLAGAEGADRIMGMFINTLPLRISL-ADRGAAEVVERTSHDLMTLLEHEQAP-----LALAQRCSGVAP 2489
Cdd:cd19535   261 PRFLLNLTLFNRLPLHPDVNDVVGDFTSLLLLEVDGsEGQSFLERARRLQQQLWEDLDHSSYSgvvvvRRLLRRRGGQPV 340


                  ...
gi 641744967 2490 PMP 2492
Cdd:cd19535   341 LAP 343
PRK05857 PRK05857
fatty acid--CoA ligase;
467-965 1.00e-12

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 73.89  E-value: 1.00e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  467 ADFPREMLiqQRFEAQAEQTPEAIAVLFED--QHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGIL 544
Cdd:PRK05857   10 PQLPSTVL--DRVFEQARQQPEAIALRRCDgtSALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  545 KAGAAYVPMDPAYPAERLAYILDDAAPVALLTQ------SAQVAQLNSTLPTVLLDTPAAA---AC------PDTNPvvq 609
Cdd:PRK05857   88 KLGAIAVMADGNLPIAAIERFCQITDPAAALVApgskmaSSAVPEALHSIPVIAVDIAAVTresEHsldaasLAGNA--- 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  610 GLHAAHLAYVIYTSGSTGRPKGVMVAHRnvinlatglhTLLALdhPSrialnasiVFDASVKNWIQLLSGHTLVLVPDAL 689
Cdd:PRK05857  165 DQGSEDPLAMIFTSGTTGEPKAVLLANR----------TFFAV--PD--------ILQKEGLNWVTWVVGETTYSPLPAT 224

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  690 RADAhqLWRyfarhavdLFDCtpvqlqwLLDAGL---GSDPAYQPAQVLIGGEA----ISPAVWSRLQS----------- 751
Cdd:PRK05857  225 HIGG--LWW--------ILTC-------LMHGGLcvtGGENTTSLLEILTTNAVattcLVPTLLSKLVSelksanatvps 287

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  752 ------------LSDTRFI--------NVYGPTE--CTvdaTACVVDRTQPLPTI-----GKPLANTRLYILDAQ--DQP 802
Cdd:PRK05857  288 lrlvgyggsraiAADVRFIeatgvrtaQVYGLSEtgCT---ALCLPTDDGSIVKIeagavGRPYPGVDVYLAATDgiGPT 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  803 VPIGVT----GELHIGGAGVARGYLHRPDLTAERFIPDPFSadpaariykTGDLARWLPDGNIDYLGRNDFQIKVRGFRI 878
Cdd:PRK05857  365 APGAGPsasfGTLWIKSPANMLGYWNNPERTAEVLIDGWVN---------TGDLLERREDGFFYIKGRSSEMIICGGVNI 435

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  879 EAGEIESRLLRCPGVQDAVVIAREDsPGDTRLVAyLCARPDAELHPAA---LRQQLAA-------SLADymiPSAFVTLD 948
Cdd:PRK05857  436 APDEVDRIAEGVSGVREAACYEIPD-EEFGALVG-LAVVASAELDESAaraLKHTIAArfrresePMAR---PSTIVIVT 510

                         570
                  ....*....|....*..
gi 641744967  949 ALPLTPNGKLDRKALPA 965
Cdd:PRK05857  511 DIPRTQSGKVMRASLAA 527
PRK03584 PRK03584
acetoacetate--CoA ligase;
1540-2016 1.58e-12

acetoacetate--CoA ligase;


Pssm-ID: 235134 [Multi-domain]  Cd Length: 655  Bit Score: 73.68  E-value: 1.58e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1540 RTPDTTAVLF--EDQH---LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAI--LKA---------GA 1603
Cdd:PRK03584   96 RRDDRPAIIFrgEDGPrreLSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATasLGAiwsscspdfGV 175

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1604 AYVpLD-------------PGYP--------AERLAYM---LDDASPVALLTQANQRALLTGDVPRILLDtaDFSHLSED 1659
Cdd:PRK03584  176 QGV-LDrfgqiepkvliavDGYRyggkafdrRAKVAELraaLPSLEHVVVVPYLGPAAAAAALPGALLWE--DFLAPAEA 252

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1660 NPHVPG-LDAHHLAYVIYTSGSTGKPKGVMNSHR-ALCNRLVWMQNTYRLTPDDRVLQKTPFS-----FDVSVweffwpL 1732
Cdd:PRK03584  253 AELEFEpVPFDHPLWILYSSGTTGLPKCIVHGHGgILLEHLKELGLHCDLGPGDRFFWYTTCGwmmwnWLVSG------L 326

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1733 LYGARLVMArpDG---HKDAAYLAQLIERTGITTL----HFVPSMLQQFVQWADaDCACDSLRRVICSGEALPAELQQRF 1805
Cdd:PRK03584  327 LVGATLVLY--DGspfYPDPNVLWDLAAEEGVTVFgtsaKYLDACEKAGLVPGE-THDLSALRTIGSTGSPLPPEGFDWV 403

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1806 FARFNAQLH--NLYGPTeaaiDVTfwACqpddhrsFVpIGRPIantqlyildtlgQPVplgVAGELHIGGVGVA------ 1877
Cdd:PRK03584  404 YEHVKADVWlaSISGGT----DIC--SC-------FV-GGNPL------------LPV---YRGEIQCRGLGMAveawde 454

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1878 RGylnRPdLTAER---FIPDPFINQP--------GARLYKT-----------GDLARWLPDGSLEYLGRNDFQVKLRGFR 1935
Cdd:PRK03584  455 DG---RP-VVGEVgelVCTKPFPSMPlgfwndpdGSRYRDAyfdtfpgvwrhGDWIEITEHGGVVIYGRSDATLNRGGVR 530

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1936 IELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVTPDPA---DLRQQLGQHLAEYMVPGAFVTLDAFPLTP 2012
Cdd:PRK03584  531 IGTAEIYRQVEALPEVLDSLVIGQEWPDGDVRMPLFVVLAEGVTLDDAlraRIRTTIRTNLSPRHVPDKIIAVPDIPRTL 610


                  ....
gi 641744967 2013 NGKL 2016
Cdd:PRK03584  611 SGKK 614
prpE PRK10524
propionyl-CoA synthetase; Provisional
 480-965 1.75e-12

propionyl-CoA synthetase; Provisional


Pssm-ID: 182517 [Multi-domain]  Cd Length: 629  Bit Score: 73.44  E-value: 1.75e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  480 EAQAEQtPEAIAVLFE---DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPA 556
Cdd:PRK10524   64 AKRPEQ-LALIAVSTEtdeERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGG 142

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  557 YPAERLAYILDDAAPVALLTQSA----------------QVAQLNSTLPTVLLDTPAAAACP-----------------D 603
Cdd:PRK10524  143 FASHSLAARIDDAKPVLIVSADAgsrggkvvpykplldeAIALAQHKPRHVLLVDRGLAPMArvagrdvdyatlraqhlG 222

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  604 TNPVVQGLHAAHLAYVIYTSGSTGRPKGVmvaHRNvinlaTGLHTllaldhpsrIALNASI-----------VFDASVKN 672
Cdd:PRK10524  223 ARVPVEWLESNEPSYILYTSGTTGKPKGV---QRD-----TGGYA---------VALATSMdtifggkagetFFCASDIG 285

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  673 WI---------QLLSGHTLVL---VPdaLRADAHQLWRYFARHAVDLFDCTPVQL--------QWLLDAGLGS------- 725
Cdd:PRK10524  286 WVvghsyivyaPLLAGMATIMyegLP--TRPDAGIWWRIVEKYKVNRMFSAPTAIrvlkkqdpALLRKHDLSSlralfla 363

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  726 -DPAYQPAQVLIGgEAISPAVwsrlqslsdtrfINVYGPTEC---TVDATACVVDRTQPLPTIGKPLANTRLYILDAQD- 800
Cdd:PRK10524  364 gEPLDEPTASWIS-EALGVPV------------IDNYWQTETgwpILAIARGVEDRPTRLGSPGVPMYGYNVKLLNEVTg 430

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  801 QPVPIGVTGELHIGGAgVARGYLH---RPDltaERFIPDPFSA-DPAarIYKTGDLARWLPDGNIDYLGRNDFQIKVRGF 876
Cdd:PRK10524  431 EPCGPNEKGVLVIEGP-LPPGCMQtvwGDD---DRFVKTYWSLfGRQ--VYSTFDWGIRDADGYYFILGRTDDVINVAGH 504

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  877 RIEAGEIESRLLRCPGVQDAVVIAREDS-----------PGDTRLVAYLCARPDAElhpAALRQQLAASLADYMIPSAFV 945
Cdd:PRK10524  505 RLGTREIEESISSHPAVAEVAVVGVKDAlkgqvavafvvPKDSDSLADREARLALE---KEIMALVDSQLGAVARPARVW 581

                         570       580
                  ....*....|....*....|
gi 641744967  946 TLDALPLTPNGKLDRKALPA 965
Cdd:PRK10524  582 FVSALPKTRSGKLLRRAIQA 601
PRK12476 PRK12476
putative fatty-acid--CoA ligase; Provisional
 2632-3096 2.12e-12

putative fatty-acid--CoA ligase; Provisional


Pssm-ID: 171527 [Multi-domain]  Cd Length: 612  Bit Score: 72.85  E-value: 2.12e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFgVRPDERVAICVERGLDMVVGLLGILKAGGAYVPL-DPTYP--VERLRYMLDDAKPVALI 2708
Cdd:PRK12476   69 LTWTQLGVRLRAVGARLQQV-AGPGDRVAILAPQGIDYVAGFFAAIKAGTIAVPLfAPELPghAERLDTALRDAEPTVVL 147

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2709 SQSAHLGIMNGSLpvillddgETRPFDNEP-----DTPLDARKQGLTPRHL-----AYVIYTSGSTGKPKGVMVEHANMV 2778
Cdd:PRK12476  148 TTTAAAEAVEGFL--------RNLPRLRRPrviaiDAIPDSAGESFVPVELdtddvSHLQYTSGSTRPPVGVEITHRAVG 219

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2779 NFLCSMRKEPGIAQEDVlLGVTSLSF--DISILEIFLPLLNGARLILATqaqaadaqqlamlierhAVSFMQaTPSTW-R 2855
Cdd:PRK12476  220 TNLVQMILSIDLLDRNT-HGVSWLPLyhDMGLSMIGFPAVYGGHSTLMS-----------------PTAFVR-RPQRWiK 280

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2856 MLVE----LRDFALPPGFK-ALCGGEALPENLATALLQKVTTL--------------------WNL--------YGPTET 2902
Cdd:PRK12476  281 ALSEgsrtGRVVTAAPNFAyEWAAQRGLPAEGDDIDLSNVVLIigsepvsidavttfnkafapYGLprtafkpsYGIAEA 360

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2903 TIW-STLN-----------------GLTTPTPY----------IGHPIANTQIYILDA-QGRVVPLGVAGEIHIAGAGVV 2953
Cdd:PRK12476  361 TLFvATIApdaepsvvyldreqlgaGRAVRVAAdapnavahvsCGQVARSQWAVIVDPdTGAELPDGEVGEIWLHGDNIG 440

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2954 RGYLGRPDLTAERFITDPFS----------GAPEARMYKTGDLGRWLpDGTLEYLGRNDFQVKVRGfrielgeietrlaR 3023
Cdd:PRK12476  441 RGYWGRPEETERTFGAKLQSrlaegshadgAADDGTWLRTGDLGVYL-DGELYITGRIADLIVIDG-------------R 506

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3024 CHGVHDAVVIAREDSP---------------GDKRLVAYLLAQPDTV-LEPADLRQRLSEGVA-EYMIPSA---FVTLDA 3083
Cdd:PRK12476  507 NHYPQDIEATVAEASPmvrrgyvtaftvpaeDNERLVIVAERAAGTSrADPAPAIDAIRAAVSrRHGLAVAdvrLVPAGA 586

                         570
                  ....*....|...
gi 641744967 3084 FPLTPNGKLDRKA 3096
Cdd:PRK12476  587 IPRTTSGKLARRA 599
PLN02654 PLN02654
acetate-CoA ligase
 490-963 2.40e-12

acetate-CoA ligase


Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 73.01  E-value: 2.40e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  490 IAVLFE------DQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLA 563
Cdd:PLN02654  106 IAIYWEgnepgfDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAESLA 185

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  564 YILDDAAPVALLTQSA-----QVAQLNSTLPTVLLDTP----AAAAC----------------------------PD--T 604
Cdd:PLN02654  186 QRIVDCKPKVVITCNAvkrgpKTINLKDIVDAALDESAkngvSVGICltyenqlamkredtkwqegrdvwwqdvvPNypT 265

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  605 NPVVQGLHAAHLAYVIYTSGSTGRPKGVMvaHRN---VINLATGLHtlLALD-HPSRI---ALNASIVFDASVKNWIQLL 677
Cdd:PLN02654  266 KCEVEWVDAEDPLFLLYTSGSTGKPKGVL--HTTggyMVYTATTFK--YAFDyKPTDVywcTADCGWITGHSYVTYGPML 341

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  678 SGHTLVLVPDALR-ADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQV-LIG--GEAISPAVWSrlqsls 753
Cdd:PLN02654  342 NGATVLVFEGAPNyPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLrVLGsvGEPINPSAWR------ 415

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  754 dtRFINVYGPTECTVDATACVVDR----TQPLP--------TIGKPLANTRLYILDAQDQPVPIGVTGELHIGGA--GVA 819
Cdd:PLN02654  416 --WFFNVVGDSRCPISDTWWQTETggfmITPLPgawpqkpgSATFPFFGVQPVIVDEKGKEIEGECSGYLCVKKSwpGAF 493

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  820 RGYLHrpdlTAERFIPDPFSadPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVI 899
Cdd:PLN02654  494 RTLYG----DHERYETTYFK--PFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVV 567

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  900 AREDSPGDTRLVAYLCARpDAELHPAALRQQLAASLADYMipSAFVTLDA------LPLTPNGKLDRKAL 963
Cdd:PLN02654  568 GIEHEVKGQGIYAFVTLV-EGVPYSEELRKSLILTVRNQI--GAFAAPDKihwapgLPKTRSGKIMRRIL 634
AcpP COG0236
Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the ...
 3112-3187 2.86e-12

Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the Pathway/BioSystem: Fatty acid biosynthesis


Pssm-ID: 440006 [Multi-domain]  Cd Length: 80  Bit Score: 64.49  E-value: 2.86e-12
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 3112 APQGDLEHALAQIWQTLLGV--ERVGRHDHFF-ELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILAAQ 3187
Cdd:COG0236     1 MPREELEERLAEIIAEVLGVdpEEITPDDSFFeDLGLDSLDAVELIAALEEEFGIELPDTELFEYPTVADLADYLEEKL 79
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
2755-3097 3.55e-12

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 70.84  E-value: 3.55e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2755 AYVIYTSGSTGKPKGVMVEHANMV--------------NFLCSMrkePG--IAQEDVLL-----GVTSLSFDISilEIFL 2813
Cdd:PRK07824   38 ALVVATSGTTGTPKGAMLTAAALTasadathdrlggpgQWLLAL---PAhhIAGLQVLVrsviaGSEPVELDVS--AGFD 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2814 PllngarlilatqaQAADAQQLAMLIERHAVSF--MQATpstwRMLVELRDFALPPGFKA-LCGGEALPENLATALLQKV 2890
Cdd:PRK07824  113 P-------------TALPRAVAELGGGRRYTSLvpMQLA----KALDDPAATAALAELDAvLVGGGPAPAPVLDAAAAAG 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2891 TTLWNLYGPTETTiwstlNGLTtptpYIGHPIANTQIYILDaqgrvvplgvaGEIHIAGAGVVRGYLGRPDltaerfiTD 2970
Cdd:PRK07824  176 INVVRTYGMSETS-----GGCV----YDGVPLDGVRVRVED-----------GRIALGGPTLAKGYRNPVD-------PD 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2971 PFSgapEARMYKTGDLGRwLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLA 3050
Cdd:PRK07824  229 PFA---EPGWFRTDDLGA-LDDGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVG 304

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*..
gi 641744967 3051 QPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK07824  305 DGGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRAL 351
LC_FACS_bac1 cd17641
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ...
 1552-1973 3.65e-12

bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341296 [Multi-domain]  Cd Length: 569  Bit Score: 72.07  E-value: 3.65e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1552 QHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLT 1631
Cdd:cd17641    10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1632 ----QANQRALLTGDVPRI------------------LLDTADFSHLSE--DNPHvPGL--------DAHHLAYVIYTSG 1679
Cdd:cd17641    90 edeeQVDKLLEIADRIPSVryviycdprgmrkyddprLISFEDVVALGRalDRRD-PGLyerevaagKGEDVAVLCTTSG 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1680 STGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTP--------FSFDVSVWE------------------------ 1727
Cdd:cd17641   169 TTGKPKLAMLSHGNFLGHCAAYLAADPLGPGDEYVSVLPlpwigeqmYSVGQALVCgfivnfpeepetmmedlreigptf 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1728 -FFWPLLYGARL--VMARpdgHKDAAYLAQLIERTGI--------TTLHFVPSMLQQFVQWADAD----------CACDS 1786
Cdd:cd17641   249 vLLPPRVWEGIAadVRAR---MMDATPFKRFMFELGMklglraldRGKRGRPVSLWLRLASWLADallfrplrdrLGFSR 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1787 LRRVICSGEALPAELQqRFFARFNAQLHNLYGPTEAAIDVTFwacQPDDHRSFVPIGRPIANTQLYILDTlgqpvplgva 1866
Cdd:cd17641   326 LRSAATGGAALGPDTF-RFFHAIGVPLKQLYGQTELAGAYTV---HRDGDVDPDTVGVPFPGTEVRIDEV---------- 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1867 GELHIGGVGVARGYLNRPDLTAERFIPDPFInqpgarlyKTGDLARWLPDGSLEYLGR-NDFQVKLRGFRIELGEIEARL 1945
Cdd:cd17641   392 GEILVRSPGVFVGYYKNPEATAEDFDEDGWL--------HTGDAGYFKENGHLVVIDRaKDVGTTSDGTRFSPQFIENKL 463

                         490       500
                  ....*....|....*....|....*...
gi 641744967 1946 MQCPGVQEAVVVARedspGDTRLVAYLC 1973
Cdd:cd17641   464 KFSPYIAEAVVLGA----GRPYLTAFIC 487
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
 712-964 4.58e-12

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 71.18  E-value: 4.58e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  712 PVQLQWLLDAglgsDPAY--QPAQVLIGGeaiSPAvWSRLQSLSDTRFINV---YGPTEctvdaTA---CVVDRTQPLP- 782
Cdd:PRK07445  215 PTQLQRLLQL----RPQWlaQFRTILLGG---APA-WPSLLEQARQLQLRLaptYGMTE-----TAsqiATLKPDDFLAg 281

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  783 --TIGKPLANTRLYILDAQdqpvpigvTGELHIGGAGVARGYLhrPDLTAerfipdpfsadpAARIYKTGDLARWLPDGN 860
Cdd:PRK07445  282 nnSSGQVLPHAQITIPANQ--------TGNITIQAQSLALGYY--PQILD------------SQGIFETDDLGYLDAQGY 339

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  861 IDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAylCARPDA-ELHPAALRQQLAASLADYM 939
Cdd:PRK07445  340 LHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTA--IYVPKDpSISLEELKTAIKDQLSPFK 417

                         250       260
                  ....*....|....*....|....*
gi 641744967  940 IPSAFVTLDALPLTPNGKLDRKALP 964
Cdd:PRK07445  418 QPKHWIPVPQLPRNPQGKINRQQLQ 442
AMP-binding_C pfam13193
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ...
 3016-3091 5.32e-12

AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.


Pssm-ID: 463804 [Multi-domain]  Cd Length: 76  Bit Score: 63.72  E-value: 5.32e-12
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967  3016 EIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGK 3091
Cdd:pfam13193    1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
Ac_CoA_lig_AcsA TIGR02188
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ...
 2593-3097 6.26e-12

acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.


Pssm-ID: 274022 [Multi-domain]  Cd Length: 626  Bit Score: 71.51  E-value: 6.26e-12
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2593 VDFNATDadipRHALIHelfeaqvactPDAIAVVF-GE-----ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERG 2666
Cdd:TIGR02188   58 VSYNCVD----RHLEAR----------PDKVAIIWeGDepgevRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMI 123

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2667 LDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQSAHL---------GIMNGSL---------------- 2721
Cdd:TIGR02188  124 PEAAIAMLACARIGAIHSVVFGGFSAEALADRINDAGAKLVITADEGLrggkviplkAIVDEALekcpvsvehvlvvrrt 203

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2722 --PVILLDDGETRPFDNE--------PDTPLDARkqgltprHLAYVIYTSGSTGKPKGVMveHAN---MVNFLCSMRKEP 2788
Cdd:TIGR02188  204 gnPVVPWVEGRDVWWHDLmakasaycEPEPMDSE-------DPLFILYTSGSTGKPKGVL--HTTggyLLYAAMTMKYVF 274

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2789 GIAQEDVL-----LG-VTSLSFdisilEIFLPLLNGA-RLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELr 2861
Cdd:TIGR02188  275 DIKDGDIFwctadVGwITGHSY-----IVYGPLANGAtTVMFEGVPTYPDPGRFWEIIEKHKVTIFYTAPTAIRALMRL- 348

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2862 dfalppgfkalcgGEALPENLATALLQKVTTL--------WNLY----GPTETTI----WSTLNG--LTTPTPYI----- 2918
Cdd:TIGR02188  349 -------------GDEWVKKHDLSSLRLLGSVgepinpeaWMWYykvvGKERCPIvdtwWQTETGgiMITPLPGAtptkp 415

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2919 ---GHPIANTQIYILDAQGRVVP-LGVAGEIHIAGA--GVVRGYLGRPdltaERFITDPFSGAPEarMYKTGDLGRWLPD 2992
Cdd:TIGR02188  416 gsaTLPFFGIEPAVVDEEGNPVEgPGEGGYLVIKQPwpGMLRTIYGDH----ERFVDTYFSPFPG--YYFTGDGARRDKD 489

                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  2993 GTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLL----AQPDTVLEpADLRQRLSEG 3068
Cdd:TIGR02188  490 GYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTlkdgYEPDDELR-KELRKHVRKE 568

                          570       580
                   ....*....|....*....|....*....
gi 641744967  3069 VAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:TIGR02188  569 IGPIAKPDKIRFVPGLPKTRSGKIMRRLL 597
PRK06814 PRK06814
acyl-[ACP]--phospholipid O-acyltransferase;
1667-2017 1.68e-11

acyl-[ACP]--phospholipid O-acyltransferase;


Pssm-ID: 235865 [Multi-domain]  Cd Length: 1140  Bit Score: 70.76  E-value: 1.68e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1667 DAHHLAYVIYTSGSTGKPKGVMNSHRA-LCNRlvwMQNTYR--LTPDDRVLQKTPF--SFDVSVwEFFWPLLYGARLVMA 1741
Cdd:PRK06814  791 DPDDPAVILFTSGSEGTPKGVVLSHRNlLANR---AQVAARidFSPEDKVFNALPVfhSFGLTG-GLVLPLLSGVKVFLY 866

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1742 RPDGHkdaaY--LAQLIERTGITTLHFVPSMLQQFVQWADA-DCAcdSLRRVICSGEALPAELQQRFFARFNAQLHNLYG 1818
Cdd:PRK06814  867 PSPLH----YriIPELIYDTNATILFGTDTFLNGYARYAHPyDFR--SLRYVFAGAEKVKEETRQTWMEKFGIRILEGYG 940

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1819 PTEAAIDVtfwACQPDDHRSFVPIGR--PIANTQLyildtlgQPVPlGV--AGELHIGGVGVARGYLnRPDltaerfipD 1894
Cdd:PRK06814  941 VTETAPVI---ALNTPMHNKAGTVGRllPGIEYRL-------EPVP-GIdeGGRLFVRGPNVMLGYL-RAE--------N 1000

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1895 PFINQPGAR-LYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIE---ARLMqcPGVQEAvVVAREDSPGDTRLVa 1970
Cdd:PRK06814 1001 PGVLEPPADgWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEelaAELW--PDALHA-AVSIPDARKGERII- 1076

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*..
gi 641744967 1971 YLCPQPGVTPDpADLRQQLGQHLAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:PRK06814 1077 LLTTASDATRA-AFLAHAKAAGASELMVPAEIITIDEIPLLGTGKID 1122
PRK09192 PRK09192
fatty acyl-AMP ligase;
2632-2779 2.20e-11

fatty acyl-AMP ligase;


Pssm-ID: 236403 [Multi-domain]  Cd Length: 579  Bit Score: 69.65  E-value: 2.20e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLD-PT-------YpVERLRYMLDDAK 2703
Cdd:PRK09192   50 LPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGLVPVPLPlPMgfggresY-IAQLRGMLASAQ 128

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2704 PVALISQSAHLGIMN---GSLP---VILLDDGETRPfdnEPDTPLdarkQGLTPRHLAYVIYTSGSTGKPKGVMVEH-AN 2776
Cdd:PRK09192  129 PAAIITPDELLPWVNeatHGNPllhVLSHAWFKALP---EADVAL----PRPTPDDIAYLQYSSGSTRFPRGVIITHrAL 201


                  ...
gi 641744967 2777 MVN 2779
Cdd:PRK09192  202 MAN 204
prpE PRK10524
propionyl-CoA synthetase; Provisional
 1529-2023 2.45e-11

propionyl-CoA synthetase; Provisional


Pssm-ID: 182517 [Multi-domain]  Cd Length: 629  Bit Score: 69.59  E-value: 2.45e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1529 LIHQLVEDQAARTPDTTAVLF------EDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAG 1602
Cdd:PRK10524   54 LCHNAVDRHLAKRPEQLALIAvstetdEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIG 133

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1603 AAYVPLDPGYPAERLAYMLDDASPV------------------ALLTQANQRA-------LLTG-------DVPRILLDT 1650
Cdd:PRK10524  134 AIHSVVFGGFASHSLAARIDDAKPVlivsadagsrggkvvpykPLLDEAIALAqhkprhvLLVDrglapmaRVAGRDVDY 213

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1651 ADFSHLSEDNpHVP--GLDAHHLAYVIYTSGSTGKPKGVmnsHR-------ALCNRlvwMQNTYRLTPDDrvlqkTPFSF 1721
Cdd:PRK10524  214 ATLRAQHLGA-RVPveWLESNEPSYILYTSGTTGKPKGV---QRdtggyavALATS---MDTIFGGKAGE-----TFFCA 281

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1722 -DVSvweffW----------PLLYGARLVM-----ARPdghkDAAYLAQLIERTGITTLHFVPS---MLQQFvqwaDADC 1782
Cdd:PRK10524  282 sDIG-----WvvghsyivyaPLLAGMATIMyeglpTRP----DAGIWWRIVEKYKVNRMFSAPTairVLKKQ----DPAL 348

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1783 A----CDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEaaidvTFW---ACQP--DD--HRSFVPiGRPIANTQL 1851
Cdd:PRK10524  349 LrkhdLSSLRALFLAGEPLDEPTASWISEALGVPVIDNYWQTE-----TGWpilAIARgvEDrpTRLGSP-GVPMYGYNV 422

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1852 YILDTL-GQPVPLGVAGELHIGGvGVARGYLN---RPDltaERFIPDPF--INQPgarLYKTGDLARWLPDGSLEYLGRN 1925
Cdd:PRK10524  423 KLLNEVtGEPCGPNEKGVLVIEG-PLPPGCMQtvwGDD---DRFVKTYWslFGRQ---VYSTFDWGIRDADGYYFILGRT 495

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1926 DFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCP-QPGVTPDP-------ADLRQQLGQHLAEYM 1997
Cdd:PRK10524  496 DDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPkDSDSLADRearlaleKEIMALVDSQLGAVA 575

                         570       580
                  ....*....|....*....|....*.
gi 641744967 1998 VPGAFVTLDAFPLTPNGKLDRKALPA 2023
Cdd:PRK10524  576 RPARVWFVSALPKTRSGKLLRRAIQA 601
ttLC_FACS_like cd05915
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
 494-963 2.81e-11

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.


Pssm-ID: 213283 [Multi-domain]  Cd Length: 509  Bit Score: 69.00  E-value: 2.81e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  494 FEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVA 573
Cdd:cd05915    20 GEVHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKV 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  574 LLTQSA--QVAQLNSTLPTVLLDTPA------------AAACPDTNPVVQGLHAAHLAyVIYTSGSTGRPKGVMVAHRNV 639
Cdd:cd05915   100 LLFDPNllPLVEAIRGELKTVQHFVVmdekapegylayEEALGEEADPVRVPERAACG-MAYTTGTTGLPKGVVYSHRAL 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  640 INLATGLHTLLALDH-PSRIALNASIVFDASVKNWIQLLSGHTLVLVPDALRADAHQLWRYFARHAVDLFDCTPVQLQWL 718
Cdd:cd05915   179 VLHSLAASLVDGTALsEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGPRLDPASLVELFDGEGVTFTAGVPTVWLAL 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  719 LDAGLGSDPAYQ-PAQVLIGGEAiSPAVWSRLQSLSDTRFINVYGPTECTVDATACV-VDRTQPLPTIGKPLANTR---- 792
Cdd:cd05915   259 ADYLESTGHRLKtLRRLVVGGSA-APRSLIARFERMGVEVRQGYGLTETSPVVVQNFvKSHLESLSEEEKLTLKAKtglp 337

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  793 -----LYILDAQDQPVP-IGVTGE-LHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLG 865
Cdd:cd05915   338 iplvrLRVADEEGRPVPkDGKALGeVQLKGPWITGGYYGNEEATRSALTPDGF--------FRTGDIAVWDEEGYVEIKD 409

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  866 RNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARpDAELHPAALRQQLAASLADY-MIPSAF 944
Cdd:cd05915   410 RLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPR-GEKPTPEELNEHLLKAGFAKwQLPDAY 488

                         490
                  ....*....|....*....
gi 641744967  945 VTLDALPLTPNGKLDRKAL 963
Cdd:cd05915   489 VFAEEIPRTSAGKFLKRAL 507
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
 1544-2020 2.94e-11

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 69.25  E-value: 2.94e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1544 TTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDD 1623
Cdd:PRK07768   20 VTGEPDAPVRHTWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAVWAED 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1624 ASPVALLTQAnqRALLTGD-----VPriLLDTADFSHLS------EDNPHVPGLDAHHLAYVIYTSGSTGKPKGVMNSHR 1692
Cdd:PRK07768  100 TLRVIGMIGA--KAVVVGEpflaaAP--VLEEKGIRVLTvadllaADPIDPVETGEDDLALMQLTSGSTGSPKAVQITHG 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1693 ALCNRLVWMQNTYRLTPD-DRVLQKTPFSFDVSVWEFFW-PLLYGARLVMARP-DGHKDAAYLAQLIERTGITTL---HF 1766
Cdd:PRK07768  176 NLYANAEAMFVAAEFDVEtDVMVSWLPLFHDMGMVGFLTvPMYFGAELVKVTPmDFLRDPLLWAELISKYRGTMTaapNF 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1767 VPSMLQQFVQWADADCACD--SLRRVICSGEALPAELQQRFF---ARFN---AQLHNLYGPTEAAIDVTFWAC------- 1831
Cdd:PRK07768  256 AYALLARRLRRQAKPGAFDlsSLRFALNGAEPIDPADVEDLLdagARFGlrpEAILPAYGMAEATLAVSFSPCgaglvvd 335

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1832 ----------------QPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYlnrpdLTAERFIPdp 1895
Cdd:PRK07768  336 evdadllaalrravpaTKGNTRRLATLGPPLPGLEVRVVDEDGQVLPPRGVGVIELRGESVTPGY-----LTMDGFIP-- 408

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1896 fiNQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVA-RED--SPGDTRLVAYl 1972
Cdd:PRK07768  409 --AQDADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAAARVEGVRPGNAVAvRLDagHSREGFAVAV- 485

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1973 cpQPGVTPDPADLRQQLGQHLAEY-----MVPGAFVTLDA--FPLTPNGKLDRKA 2020
Cdd:PRK07768  486 --ESNAFEDPAEVRRIRHQVAHEVvaevgVRPRNVVVLGPgsIPKTPSGKLRRAN 538
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
 498-962 4.14e-11

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 68.48  E-value: 4.14e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  498 HLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAyVPMDPAyPAER--LA-YILDDAAPVAL 574
Cdd:PRK07768   29 RHTWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIAPTAQGLWMRGAS-LTMLHQ-PTPRtdLAvWAEDTLRVIGM 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  575 LTQSAQVA-----QLNSTLP----TVLLDTPAAAACPdTNPVVQGLHAahLAYVIYTSGSTGRPKGVMVAHRNVINLATG 645
Cdd:PRK07768  107 IGAKAVVVgepflAAAPVLEekgiRVLTVADLLAADP-IDPVETGEDD--LALMQLTSGSTGSPKAVQITHGNLYANAEA 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  646 LHTLLALDHPSRIALN---------------------ASIV------FDASVKNWIQLLSGH--TLVLVPD-ALRADAHQ 695
Cdd:PRK07768  184 MFVAAEFDVETDVMVSwlplfhdmgmvgfltvpmyfgAELVkvtpmdFLRDPLLWAELISKYrgTMTAAPNfAYALLARR 263

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  696 LWRYFARHAVDLfdctpVQLQWlldaglgsdpayqpaqVLIGGEAISPAVWSRLQS------LSDTRFINVYG------- 762
Cdd:PRK07768  264 LRRQAKPGAFDL-----SSLRF----------------ALNGAEPIDPADVEDLLDagarfgLRPEAILPAYGmaeatla 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  763 ---------PTECTVDATACVVDR---------TQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYlh 824
Cdd:PRK07768  323 vsfspcgagLVVDEVDADLLAALRravpatkgnTRRLATLGPPLPGLEVRVVDEDGQVLPPRGVGVIELRGESVTPGY-- 400

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  825 rpdLTAERFIPdpfsADPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDS 904
Cdd:PRK07768  401 ---LTMDGFIP----AQDADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAAARVEGVRPGNAVAVRLD 473

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967  905 PGDTR----LVAYLCARPDAElHPAALRQQLAASLADY--MIPSAFVTLDA--LPLTPNGKLDRKA 962
Cdd:PRK07768  474 AGHSRegfaVAVESNAFEDPA-EVRRIRHQVAHEVVAEvgVRPRNVVVLGPgsIPKTPSGKLRRAN 538
PRK08180 PRK08180
feruloyl-CoA synthase; Reviewed
 2632-3072 5.48e-11

feruloyl-CoA synthase; Reviewed


Pssm-ID: 236175 [Multi-domain]  Cd Length: 614  Bit Score: 68.37  E-value: 5.48e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPV-----ERLRYML------- 2699
Cdd:PRK08180   70 LTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEHALLALAAMYAGVPYAPVSPAYSLvsqdfGKLRHVLelltpgl 149

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 ---DDAKPVAlisqSAHLGIMNGSLPVIL----LDDGETRPFDNEPDTP----LDARKQGLTPRHLAYVIYTSGSTGKPK 2768
Cdd:PRK08180  150 vfaDDGAAFA----RALAAVVPADVEVVAvrgaVPGRAATPFAALLATPptaaVDAAHAAVGPDTIAKFLFTSGSTGLPK 225

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2769 GVMVEHANmvnfLCS---MRKE--PGIAQEDVLLgVTSL--------SFDISILeiflpLLNGARLILATQAQAADaqql 2835
Cdd:PRK08180  226 AVINTHRM----LCAnqqMLAQtfPFLAEEPPVL-VDWLpwnhtfggNHNLGIV-----LYNGGTLYIDDGKPTPG---- 291

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2836 amLIERH---------AVSFMqaTPSTWRMLVEL--RDFALPPGF----KALC-GGEALPENLATAlLQKVT-------- 2891
Cdd:PRK08180  292 --GFDETlrnlreispTVYFN--VPKGWEMLVPAleRDAALRRRFfsrlKLLFyAGAALSQDVWDR-LDRVAeatcgeri 366

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2892 TLWNLYGPTETTIWST-LNGLTTPTPYIGHPIANTQIyildaqgRVVPLGVAGEIHIAGAGVVRGYLGRPDLTAERFITD 2970
Cdd:PRK08180  367 RMMTGLGMTETAPSATfTTGPLSRAGNIGLPAPGCEV-------KLVPVGGKLEVRVKGPNVTPGYWRAPELTAEAFDEE 439

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2971 PFsgapearmYKTGDLGRWL----PDGTLEYLGR--NDFQ------VKVRGFRIELgeietrLARCHG-VHDAVVIA--R 3035
Cdd:PRK08180  440 GY--------YRSGDAVRFVdpadPERGLMFDGRiaEDFKlssgtwVSVGPLRARA------VSAGAPlVQDVVITGhdR 505

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 3036 ED-------SPGDKRLVAYLLAQPD--TVLEPADLRQRLSEGVAEY 3072
Cdd:PRK08180  506 DEigllvfpNLDACRRLAGLLADASlaEVLAHPAVRAAFRERLARL 551
PLN02387 PLN02387
long-chain-fatty-acid-CoA ligase family protein
 1549-1958 6.14e-11

long-chain-fatty-acid-CoA ligase family protein


Pssm-ID: 215217 [Multi-domain]  Cd Length: 696  Bit Score: 68.60  E-value: 6.14e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1549 FEDQHL------TYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLD 1622
Cdd:PLN02387   96 FEKLHLgeyewiTYGQVFERVCNFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLN 175

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1623 DASPVALLTQANQRALLTG-------------------DVPRILLDTAD-----FSH---LSEDNPHVPGL-DAHHLAYV 1674
Cdd:PLN02387  176 ETEVTTVICDSKQLKKLIDissqletvkrviymddegvDSDSSLSGSSNwtvssFSEvekLGKENPVDPDLpSPNDIAVI 255

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1675 IYTSGSTGKPKGVMNSHRALCNRLVW-MQNTYRLTPDDRVLQKTPFSfdvSVWEFFWPLL---------YGARLVMARPD 1744
Cdd:PLN02387  256 MYTSGSTGLPKGVMMTHGNIVATVAGvMTVVPKLGKNDVYLAYLPLA---HILELAAESVmaavgaaigYGSPLTLTDTS 332

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1745 -----GHK-DAAYLaqliertGITTLHFVPSMLQQ-----------------------------------FVQWADADCA 1783
Cdd:PLN02387  333 nkikkGTKgDASAL-------KPTLMTAVPAILDRvrdgvrkkvdakgglakklfdiaykrrlaaiegswFGAWGLEKLL 405

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1784 CDSL-------------RRVICSGEALPAElQQRFFAR-FNAQLHNLYGPTEAAIDVTFwaCQPDDhRSFVPIGRPIANT 1849
Cdd:PLN02387  406 WDALvfkkiravlggriRFMLSGGAPLSGD-TQRFINIcLGAPIGQGYGLTETCAGATF--SEWDD-TSVGRVGPPLPCC 481

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1850 QLYILD-------TLGQPVPlgvAGELHIGGVGVARGYLNRPDLTAERFIPDpfinQPGARLYKTGDLARWLPDGSLEYL 1922
Cdd:PLN02387  482 YVKLVSweeggylISDKPMP---RGEIVIGGPSVTLGYFKNQEKTDEVYKVD----ERGMRWFYTGDIGQFHPDGCLEII 554

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 641744967 1923 GRNDFQVKLR-GFRIELGEIEARLMQCPGVQEAVVVA 1958
Cdd:PLN02387  555 DRKKDIVKLQhGEYVSLGKVEAALSVSPYVDNIMVHA 591
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
 2620-3097 6.27e-11

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 68.36  E-value: 6.27e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVF-GE-----ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVE 2693
Cdd:cd05966    67 GDKVAIIWeGDepdqsRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAE 146

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2694 RLRYMLDDAKPVALISQSAhlGIMNGSL------------------PVILLDD-GETRPFDNEPDTPLDARKQGLTPRHL 2754
Cdd:cd05966   147 SLADRINDAQCKLVITADG--GYRGGKViplkeivdealekcpsveKVLVVKRtGGEVPMTEGRDLWWHDLMAKQSPECE 224

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2755 A---------YVIYTSGSTGKPKGVMVEHA-NMVNFLCSMRKEPGIAQEDVL-----LG-VTSLSFdisilEIFLPLLNG 2818
Cdd:cd05966   225 PewmdsedplFILYTSGSTGKPKGVVHTTGgYLLYAATTFKYVFDYHPDDIYwctadIGwITGHSY-----IVYGPLANG 299

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2819 AR-LILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELrdfalppgfkalcgGEALPENLATALLQKVTT----- 2892
Cdd:cd05966   300 ATtVMFEGTPTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMKF--------------GDEWVKKHDLSSLRVLGSvgepi 365

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2893 -------LWNLYG----PTETTIWSTLNG--LTTPTPYI--------GHPIANTQIYILDAQGRVVPLGVAGEIHIAGA- 2950
Cdd:cd05966   366 npeawmwYYEVIGkercPIVDTWWQTETGgiMITPLPGAtplkpgsaTRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPw 445

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2951 -GVVRGYLGRPdltaERFITDPFSgaPEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHD 3029
Cdd:cd05966   446 pGMARTIYGDH----ERYEDTYFS--KFPGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAE 519

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967 3030 AVVIAREDSPGDKRLVAYLL----AQPDTVLEpADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:cd05966   520 AAVVGRPHDIKGEAIYAFVTlkdgEEPSDELR-KELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIMRRIL 590
PRK05857 PRK05857
fatty acid--CoA ligase;
2600-3109 7.29e-11

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 67.73  E-value: 7.29e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2600 ADIPRHALIHELFEAQVacTPDAIAV--VFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGIL 2677
Cdd:PRK05857   10 PQLPSTVLDRVFEQARQ--QPEAIALrrCDGTSALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2678 KAGGAYVPLDPTYPVERLRYMLDDAKPVALISQ------SAHLGIMNGSLPVILLDDG-ETRPFDNEPDTPLDARKQGLT 2750
Cdd:PRK05857   88 KLGAIAVMADGNLPIAAIERFCQITDPAAALVApgskmaSSAVPEALHSIPVIAVDIAaVTRESEHSLDAASLAGNADQG 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2751 PRHLAYVIYTSGSTGKPKGVMVehANMVNF-LCSMRKEPGIAQEDVLLGVTSLS----FDISIL-EIFLPLLNGARLIla 2824
Cdd:PRK05857  168 SEDPLAMIFTSGTTGEPKAVLL--ANRTFFaVPDILQKEGLNWVTWVVGETTYSplpaTHIGGLwWILTCLMHGGLCV-- 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2825 tqAQAADAQQLAMLIERHAVSFMQATPSTWRMLV-ELR--DFALPP-GFKALCGGEALPENLATALLQKVTTLwNLYGPT 2900
Cdd:PRK05857  244 --TGGENTTSLLEILTTNAVATTCLVPTLLSKLVsELKsaNATVPSlRLVGYGGSRAIAADVRFIEATGVRTA-QVYGLS 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2901 ETTIwstlNGLTTPTP----------YIGHPIANTQIYILDAQG------RVVPLGVAGEIHIAGAGVVRGYLGRPDLTA 2964
Cdd:PRK05857  321 ETGC----TALCLPTDdgsivkieagAVGRPYPGVDVYLAATDGigptapGAGPSASFGTLWIKSPANMLGYWNNPERTA 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2965 ERFITDpfsgapearMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIE--------TRLARCHGVHD------- 3029
Cdd:PRK05857  397 EVLIDG---------WVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDriaegvsgVREAACYEIPDeefgalv 467

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3030 --AVVIARE-DSPGDKRLVAYLLAQpdtvlepadlRQRLSEGVAEymiPSAFVTLDAFPLTPNGKLDRKALPAP---DQS 3103
Cdd:PRK05857  468 glAVVASAElDESAARALKHTIAAR----------FRRESEPMAR---PSTIVIVTDIPRTQSGKVMRASLAAAataDKA 534


                  ....*.
gi 641744967 3104 AMATRG 3109
Cdd:PRK05857  535 RVVVRG 540
AcpP COG0236
Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the ...
 978-1050 1.15e-10

Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the Pathway/BioSystem: Fatty acid biosynthesis


Pssm-ID: 440006 [Multi-domain]  Cd Length: 80  Bit Score: 59.87  E-value: 1.15e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967  978 APQGGIETALAALWQELLGLD--RVGRHDQFFA-LGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAAVTA 1050
Cdd:COG0236     1 MPREELEERLAEIIAEVLGVDpeEITPDDSFFEdLGLDSLDAVELIAALEEEfGIELPDTELFEYPTVADLADYLEE 77
PTZ00237 PTZ00237
acetyl-CoA synthetase; Provisional
 613-963 1.53e-10

acetyl-CoA synthetase; Provisional


Pssm-ID: 240325 [Multi-domain]  Cd Length: 647  Bit Score: 67.07  E-value: 1.53e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  613 AAHLAYVIYTSGSTGRPKGVMVAH-RNVINLATGLHTLLALDHPSRIALNASIvfdasvkNWIQ--------LLSGHTLV 683
Cdd:PTZ00237  253 SSHPLYILYTSGTTGNSKAVVRSNgPHLVGLKYYWRSIIEKDIPTVVFSHSSI-------GWVSfhgflygsLSLGNTFV 325

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  684 LVPDALRADAH---QLWRYFARHAVDLFDCTPVQLQWLLDaglgSDP-------AYQPA---QVLIGGEAISPAVWSRLQ 750
Cdd:PTZ00237  326 MFEGGIIKNKHiedDLWNTIEKHKVTHTLTLPKTIRYLIK----TDPeatiirsKYDLSnlkEIWCGGEVIEESIPEYIE 401

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  751 SLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGgagvargyLHRPDLTA 830
Cdd:PTZ00237  402 NKLKIKSSRGYGQTEIGITYLYCYGHINIPYNATGVPSIFIKPSILSEDGKELNVNEIGEVAFK--------LPMPPSFA 473

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  831 ERFIPDP------FSADPAarIYKTGDLArwLPDGNiDYLG---RNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAR 901
Cdd:PTZ00237  474 TTFYKNDekfkqlFSKFPG--YYNSGDLG--FKDEN-GYYTivsRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGI 548

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967  902 EDSPGDTRLVAYLCARPDAELHPAALRQ---QLAASLADYMIPSAFVT----LDALPLTPNGKLDRKAL 963
Cdd:PTZ00237  549 YDPDCYNVPIGLLVLKQDQSNQSIDLNKlknEINNIITQDIESLAVLRkiiiVNQLPKTKTGKIPRQII 617
PP-binding pfam00550
Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached ...
 3119-3178 2.35e-10

Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. This family includes members not included in Prosite. The inclusion of these members is supported by sequence analysis and functional evidence. The related domain of Swiss:P19828 has the attachment serine replaced by an alanine.


Pssm-ID: 425746 [Multi-domain]  Cd Length: 62  Bit Score: 58.34  E-value: 2.35e-10
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967  3119 HALAQIWQTLLGV--ERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSA 3178
Cdd:pfam00550    1 ERLRELLAEVLGVpaEEIDPDTDLFDLGLDSLLAVELIARLEEEFGVEIPPSDLFEHPTLAE 62
PRK05620 PRK05620
long-chain fatty-acid--CoA ligase;
495-963 2.80e-10

long-chain fatty-acid--CoA ligase;


Pssm-ID: 180167 [Multi-domain]  Cd Length: 576  Bit Score: 65.96  E-value: 2.80e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  495 EDQHLTYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAP-- 571
Cdd:PRK05620   35 EQEQTTFAAIGARAAALAHALHdELGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDev 114

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  572 -VALLTQSAQVAQLNSTLPTV---------LLDTPAAAACPDTN-----------------PVVQGLHAAHLAYviyTSG 624
Cdd:PRK05620  115 iVADPRLAEQLGEILKECPCVravvfigpsDADSAAAHMPEGIKvysyealldgrstvydwPELDETTAAAICY---STG 191

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  625 STGRPKGVMVAHRNVINLATGLHTL--LALDHPSRIALNASIVfdaSVKNW----IQLLSGHTLVL-------------V 685
Cdd:PRK05620  192 TTGAPKGVVYSHRSLYLQSLSLRTTdsLAVTHGESFLCCVPIY---HVLSWgvplAAFMSGTPLVFpgpdlsaptlakiI 268

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  686 PDALRADAH-------QLWRYFARHAVDlfdctPVQLQwlldaglgsdpayqpaQVLIGGEAISPAvwsrLQSLSDTRF- 757
Cdd:PRK05620  269 ATAMPRVAHgvptlwiQLMVHYLKNPPE-----RMSLQ----------------EIYVGGSAVPPI----LIKAWEERYg 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  758 ---INVYGPTEctvdatacvvdrTQPLPTIGKPLA----NTRLYILDAQDQpVPIGV-----------------TGELHI 813
Cdd:PRK05620  324 vdvVHVWGMTE------------TSPVGTVARPPSgvsgEARWAYRVSQGR-FPASLeyrivndgqvmestdrnEGEIQV 390

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  814 GGAGVARGYLHRP----DLTAERF-------IPDPFSADPAARiykTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGE 882
Cdd:PRK05620  391 RGNWVTASYYHSPteegGGAASTFrgedvedANDRFTADGWLR---TGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQ 467

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  883 IESRLLRCPGVQDAVVIAREDSPGDTRLVAYLCARPDAELHPAA---LRQQLAASLADYMIPSAFVTLDALPLTPNGKLD 959
Cdd:PRK05620  468 LENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETaerLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFD 547


                  ....
gi 641744967  960 RKAL 963
Cdd:PRK05620  548 KKDL 551
C_PKS-NRPS cd20483
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 2163-2501 2.80e-10

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHXXXD motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380471 [Multi-domain]  Cd Length: 430  Bit Score: 65.36  E-value: 2.80e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2163 PLAPLQEGILFHHLLQEQGDTYLLRSMVAFTHRERLDAFLSALQQVIDRHDILRTAVCwQDLSQPVQVVWRQAILPINHF 2242
Cdd:cd20483     3 PMSTFQRRLWFLHNFLEDKTFLNLLLVCHIKGKPDVNLLQKALSELVRRHEVLRTAYF-EGDDFGEQQVLDDPSFHLIVI 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2243 EPTSPEDVLAQLQAHTEPRTRR-IDLSQAPLFRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEI----RLLLQHQA 2317
Cdd:cd20483    82 DLSEAADPEAALDQLVRNLRRQeLDIEEGEVIRGWLVKLP-DEEFALVLASHHIAWDRGSSKSIFEQFtalyDALRAGRD 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2318 DALPTPLPYrNFIAQTL-------SVPNSAHEAYFRDKLADVDEPTA--PFGLLN---VQGSGGDIHEARlvLDATLASA 2385
Cdd:cd20483   161 LATVPPPPV-QYIDFTLwhnallqSPLVQPLLDFWKEKLEGIPDASKllPFAKAErppVKDYERSTVEAT--LDKELLAR 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2386 IRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVLLGRlaGAEGADRIMGMFINTLPLRISLADRGAAE-VVERTS 2464
Cdd:cd20483   238 MKRICAQHAVTPFMFLLAAFRAFLYRYTEDEDLTIGMVDGDR--PHPDFDDLVGFFVNMLPIRCRMDCDMSFDdLLESTK 315

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 641744967 2465 HDLMTLLEHEQAPL-ALAQRCSgvAPP----MPLFSTLLNYR 2501
Cdd:cd20483   316 TTCLEAYEHSAVPFdYIVDALD--VPRstshFPIGQIAVNYQ 355
LCL_NRPS cd19538
LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; ...
 30-316 2.92e-10

LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380461 [Multi-domain]  Cd Length: 432  Bit Score: 65.36  E-value: 2.92e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEKGDTYllNSLLAFDSQTRLD--AFLDVLQQVIARHDILRTaiCWQGLH-QPVQVVWRQAPLTV 106
Cdd:cd19538     3 PLSFAQRRLWFLHQLEGPSATY--NIPLVIKLKGKLDvqALQQALYDVVERHESLRT--VFPEEDgVPYQLILEEDEATP 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  107 N-TLTTTSSDTVPAQLRAATdpsNHRLNLSNAPLLSATTaHDPVCGEWLLSLSIHHLISDHITQALIIDEIRLLLEDR-- 183
Cdd:cd19538    79 KlEIKEVDEEELESEINEAV---RYPFDLSEEPPFRATL-FELGENEHVLLLLLHHIAADGWSLAPLTRDLSKAYRARck 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  184 ---PEALPKPLPYRNFIA---QIL------SVPLSEHEQYFRNRLA----DIDTPTAPFDLVDVQGNGEDITearLSLDS 247
Cdd:cd19538   155 geaPELAPLPVQYADYALwqqELLgdesdpDSLIARQLAYWKKQLAglpdEIELPTDYPRPAESSYEGGTLT---FEIDS 231

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  248 SLADALRRQARHLGISSSVLFHVAWAQVLA-LTSGrDDVVFGSVLSGRLQGNLGAdrVMGMFINTLPLRV 316
Cdd:cd19538   232 ELHQQLLQLAKDNNVTLFMVLQAGFAALLTrLGAG-TDIPIGSPVAGRNDDSLED--LVGFFVNTLVLRT 298
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
 2632-3024 2.93e-10

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 65.70  E-value: 2.93e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISqs 2711
Cdd:cd17639     6 MSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIHSLNETECSAIFT-- 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2712 ahlgimngslpvillddgetrpfDNEPDTpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVNFLCSM--RKEPG 2789
Cdd:cd17639    84 -----------------------DGKPDD-------------LACIMYTSGSTGNPKGVMLTHGNLVAGIAGLgdRVPEL 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2790 IAQEDVLLGVTSLSfdiSILEI---FLPLLNGARL-----------ILATQAQAADAQQLAMLIE--------RHAVSFM 2847
Cdd:cd17639   128 LGPDDRYLAYLPLA---HIFELaaeNVCLYRGGTIgygsprtltdkSKRGCKGDLTEFKPTLMVGvpaiwdtiRKGVLAK 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2848 QATPSTWRMLV-----ELRDFALPPG----------FK------------ALCGGEALP----ENLATALLQKVTTlwnl 2896
Cdd:cd17639   205 LNPMGGLKRTLfwtayQSKLKALKEGpgtplldelvFKkvraalggrlryMLSGGAPLSadtqEFLNIVLCPVIQG---- 280

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2897 YGPTETTIWSTLNGLTTPTP-YIGHPIANTQIYILDaqgrVVPLGVA-------GEIHIAGAGVVRGYLGRPDLTAERFI 2968
Cdd:cd17639   281 YGLTETCAGGTVQDPGDLETgRVGPPLPCCEIKLVD----WEEGGYStdkppprGEILIRGPNVFKGYYKNPEKTKEAFD 356

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 2969 TDpfsgapeaRMYKTGDLGRWLPDGTLEYLGRNDFQVKVR-GFRIELGEIETRLARC 3024
Cdd:cd17639   357 GD--------GWFHTGDIGEFHPDGTLKIIDRKKDLVKLQnGEYIALEKLESIYRSN 405
C_PKS-NRPS_PksJ-like cd20484
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 57-411 3.38e-10

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs), similar to Bacillus subtilis PksJ; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily have the typical C-domain HHxxxD motif. PksJ is involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is important in secondary metabolism. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380472 [Multi-domain]  Cd Length: 430  Bit Score: 65.42  E-value: 3.38e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   57 LAFDSQTRLD--AFLDVLQQVIARHDILRTAICWQGlHQPVQVVWRQAPLTVNT--LTTTSSDTVPAQLRA-ATDPsnhr 131
Cdd:cd20484    28 LCFRFSSKLDveKFKQACQFVLEQHPILKSVIEEED-GVPFQKIEPSKPLSFQEedISSLKESEIIAYLREkAKEP---- 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  132 LNLSNAPLLSATTAHDPVcGEWLLSLSIHHLISDHITQALIIDEI----RLLLEDR-PEALPKPLPYRNFIA---QILSV 203
Cdd:cd20484   103 FVLENGPLMRVHLFSRSE-QEHFVLITIHHIIFDGSSSLTLIHSLldayQALLQGKqPTLASSPASYYDFVAweqDMLAG 181

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  204 PLSE-HEQYFRNRLA----DIDTPTAPFDLVDVQGNGEdiTEARLsLDSSLADALRRQARHLGISSSVLFHVAWAQVLAL 278
Cdd:cd20484   182 AEGEeHRAYWKQQLSgtlpILELPADRPRSSAPSFEGQ--TYTRR-LPSELSNQIKSFARSQSINLSTVFLGIFKLLLHR 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  279 TSGRDDVVFGSVLSGRLQGNLgaDRVMGMFINTLPLRVS-LRERSVHDVVQATSHELMMLLAHEQAPLALAQQCSQVPPP 357
Cdd:cd20484   259 YTGQEDIIVGMPTMGRPEERF--DSLIGYFINMLPIRSRiLGEETFSDFIRKLQLTVLDGLDHAAYPFPAMVRDLNIPRS 336

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  358 L---PLFSTLFNYRHSQKDASSQ-------------FWEGMRQlsgreRTNYPITLSVDDLGDGFNLTAK 411
Cdd:cd20484   337 QansPVFQVAFFYQNFLQSTSLQqflaeyqdvlsieFVEGIHQ-----EGEYELVLEVYEQEDRFTLNIK 401
C_PKS-NRPS_PksJ-like cd20484
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 2199-2448 5.52e-10

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs), similar to Bacillus subtilis PksJ; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily have the typical C-domain HHxxxD motif. PksJ is involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is important in secondary metabolism. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380472 [Multi-domain]  Cd Length: 430  Bit Score: 64.64  E-value: 5.52e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2199 DAFLSALQQVIDRHDILRTAVCWQDlSQPVQVVWRQAILPINHfEPTS---PEDVLAQLQAHT-EPrtrrIDLSQAPLFR 2274
Cdd:cd20484    39 EKFKQACQFVLEQHPILKSVIEEED-GVPFQKIEPSKPLSFQE-EDISslkESEIIAYLREKAkEP----FVLENGPLMR 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2275 ADIAHDPLQnEWLLALSFHHLISDHMTLALIVGEI----RLLLQHQADALPTPLP-YRNFIA---QTLSVPNS-AHEAYF 2345
Cdd:cd20484   113 VHLFSRSEQ-EHFVLITIHHIIFDGSSSLTLIHSLldayQALLQGKQPTLASSPAsYYDFVAweqDMLAGAEGeEHRAYW 191

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2346 RDKLADV--------DEPTApfgllNVQGSGGDIHEARLvlDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDD 2417
Cdd:cd20484   192 KQQLSGTlpilelpaDRPRS-----SAPSFEGQTYTRRL--PSELSNQIKSFARSQSINLSTVFLGIFKLLLHRYTGQED 264

                         250       260       270
                  ....*....|....*....|....*....|.
gi 641744967 2418 VVFGSVLLGRlaGAEGADRIMGMFINTLPLR 2448
Cdd:cd20484   265 IIVGMPTMGR--PEERFDSLIGYFINMLPIR 293
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
 1764-2022 6.29e-10

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 64.63  E-value: 6.29e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1764 LHFVPSMLQQF----VQWadadcacdsLRR----VICSGEALPAELQQrffarfnAQLHNL-----YGPTEAAIDVTfwA 1830
Cdd:PRK07445  211 LSLVPTQLQRLlqlrPQW---------LAQfrtiLLGGAPAWPSLLEQ-------ARQLQLrlaptYGMTETASQIA--T 272

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1831 CQPDD----HRSfvpIGRPIANTQLYILDtlgqpvplGVAGELHIGGVGVARGYlnrpdltaerfIPDPFINQpgaRLYK 1906
Cdd:PRK07445  273 LKPDDflagNNS---SGQVLPHAQITIPA--------NQTGNITIQAQSLALGY-----------YPQILDSQ---GIFE 327

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1907 TGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAYLCPQPGVtPDPADLR 1986
Cdd:PRK07445  328 TDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDPS-ISLEELK 406

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 641744967 1987 QQLGQHLAEYMVPGAFVTLDAFPLTPNGKLDRKALP 2022
Cdd:PRK07445  407 TAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQ 442
AACS cd05943
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ...
 2619-3091 7.07e-10

Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.


Pssm-ID: 341265 [Multi-domain]  Cd Length: 629  Bit Score: 64.98  E-value: 7.07e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVFGE----ASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAY----------- 2683
Cdd:cd05943    82 ADDPAAIYAAEdgerTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWsscspdfgvpg 161

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2684 -----VPLDPT--YPVERLRY----------------MLDDAKPVALIS--QSAHLGIMNGSLPVILLDDGETRPFDNEP 2738
Cdd:cd05943   162 vldrfGQIEPKvlFAVDAYTYngkrhdvrekvaelvkGLPSLLAVVVVPytVAAGQPDLSKIAKALTLEDFLATGAAGEL 241

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2739 D---TPLDarkqgltprHLAYVIYTSGSTGKPK-------GVMVEHANMVNFLCSMRkePGiaqeDVLLGVTSLS----- 2803
Cdd:cd05943   242 EfepLPFD---------HPLYILYSSGTTGLPKcivhgagGTLLQHLKEHILHCDLR--PG----DRLFYYTTCGwmmwn 306

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2804 FDISileiflPLLNGARLILATQAQAADAQQLAM-LIERHAVSFMQATPstwRMLVELRDFALPPgfkalcggeALPENL 2882
Cdd:cd05943   307 WLVS------GLAVGATIVLYDGSPFYPDTNALWdLADEEGITVFGTSA---KYLDALEKAGLKP---------AETHDL 368

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2883 ATalLQkvttlwnlygptetTIWSTLNGLTTPT-PYIGHPIAN----------TQIYILDAQG-RVVPLGvAGEIHIAGA 2950
Cdd:cd05943   369 SS--LR--------------TILSTGSPLKPESfDYVYDHIKPdvllasisggTDIISCFVGGnPLLPVY-RGEIQCRGL 431

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2951 GV-VRGYL--GRP--DLTAERFITDP-------FSGAPEARMYKT------------GDLGRWLPDGTLEYLGRNDFQVK 3006
Cdd:cd05943   432 GMaVEAFDeeGKPvwGEKGELVCTKPfpsmpvgFWNDPDGSRYRAayfakypgvwahGDWIEITPRGGVVILGRSDGTLN 511

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3007 VRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPA---DLRQRLSEGVAEYMIPSAFVTLDA 3083
Cdd:cd05943   512 PGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDDElrkRIRSTIRSALSPRHVPAKIIAVPD 591


                  ....*...
gi 641744967 3084 FPLTPNGK 3091
Cdd:cd05943   592 IPRTLSGK 599
Cyc_NRPS cd19535
Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 72-340 7.81e-10

Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Cyc (heterocyclization) domains catalyze two separate reactions in the creation of heterocyclized peptide products in nonribosomal peptide synthesis: amide bond formation followed by intramolecular cyclodehydration between a Cys, Ser, or Thr side chain and a carbonyl carbon on the peptide backbone to form a thiazoline, oxazoline, or methyloxazoline ring. Cyc-domains are homologous to standard NRPS Condensation (C) domains. C-domains typically have a conserved HHxxxD motif at the active site; Cyc-domains have an alternative, conserved DxxxxD active site motif, mutation of the aspartate residues in this motif can abolish or diminish condensation activity. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and Cyc-domains. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380458 [Multi-domain]  Cd Length: 423  Bit Score: 64.05  E-value: 7.81e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   72 LQQVIARHDILRTAIcwqgLHQPVQVVWRQAP---LTVNTLTTTSSDTVPAQLRAATDPSNHR-LNLSNAPLLSATTAHD 147
Cdd:cd19535    46 WNKLIARHPMLRAVF----LDDGTQQILPEVPwygITVHDLRGLSEEEAEAALEELRERLSHRvLDVERGPLFDIRLSLL 121

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  148 PVcGEWLLSLSIHHLISDHITQALIIDEIRLLLEDRPEALPK-PLPYRNFIAQILSVPLSEHEQ---YFRNRLADIdtPT 223
Cdd:cd19535   122 PE-GRTRLHLSIDLLVADALSLQILLRELAALYEDPGEPLPPlELSFRDYLLAEQALRETAYERaraYWQERLPTL--PP 198

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  224 APfDLvDVQGNGEDITEARLS-----LDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGN 298
Cdd:cd19535   199 AP-QL-PLAKDPEEIKEPRFTrrehrLSAEQWQRLKERARQHGVTPSMVLLTAYAEVLARWSGQPRFLLNLTLFNRLPLH 276

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|...
gi 641744967  299 LGADRVMGMFINTLPLRVSLRE-RSVHDVVQATSHELMMLLAH 340
Cdd:cd19535   277 PDVNDVVGDFTSLLLLEVDGSEgQSFLERARRLQQQLWEDLDH 319
PRK08162 PRK08162
acyl-CoA synthetase; Validated
 2620-3097 9.93e-10

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 64.20  E-value: 9.93e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2620 PDAIAVVFGEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML 2699
Cdd:PRK08162   32 PDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFML 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2700 DDAKPVALI-----SQSAH--LGIMNGSLP-VILLDDGETRPFDNEPDTPLDARKQGLTPrHLAYVI-----------YT 2760
Cdd:PRK08162  112 RHGEAKVLIvdtefAEVAReaLALLPGPKPlVIDVDDPEYPGGRFIGALDYEAFLASGDP-DFAWTLpadewdaialnYT 190

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2761 SGSTGKPKGVMVEH-----ANMVNFL-CSMRKEPgiaqedVLLGVtslsfdisileifLPLL--NG-------------- 2818
Cdd:PRK08162  191 SGTTGNPKGVVYHHrgaylNALSNILaWGMPKHP------VYLWT-------------LPMFhcNGwcfpwtvaaragtn 251

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2819 -------ARLILAtqaqaadaqqlamLIERHAVSFMQATPSTWRMLVELRDfALPPGF----KALCGGEALPEnlatALL 2887
Cdd:PRK08162  252 vclrkvdPKLIFD-------------LIREHGVTHYCGAPIVLSALINAPA-EWRAGIdhpvHAMVAGAAPPA----AVI 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2888 QKVTT----LWNLYGPTET----TI------WSTL-------------------NGLTTPTPYIGHPIAntqiyildAQG 2934
Cdd:PRK08162  314 AKMEEigfdLTHVYGLTETygpaTVcawqpeWDALplderaqlkarqgvryplqEGVTVLDPDTMQPVP--------ADG 385

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2935 RVVplgvaGEIHIAGAGVVRGYLGRPDLTAERFitdpfsgapEARMYKTGDLGRWLPDGtleYLgrndfQVKVR------ 3008
Cdd:PRK08162  386 ETI-----GEIMFRGNIVMKGYLKNPKATEEAF---------AGGWFHTGDLAVLHPDG---YI-----KIKDRskdiii 443

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3009 --GFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVAYLLAQPDTVLEPADLRQRLSEGVAEYMIPSAfVTLDAFPL 3086
Cdd:PRK08162  444 sgGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEEEIIAHCREHLAGFKVPKA-VVFGELPK 522

                         570
                  ....*....|.
gi 641744967 3087 TPNGKLDRKAL 3097
Cdd:PRK08162  523 TSTGKIQKFVL 533
PLN02387 PLN02387
long-chain-fatty-acid-CoA ligase family protein
 2632-2811 1.05e-09

long-chain-fatty-acid-CoA ligase family protein


Pssm-ID: 215217 [Multi-domain]  Cd Length: 696  Bit Score: 64.37  E-value: 1.05e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQS 2711
Cdd:PLN02387  107 ITYGQVFERVCNFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLNETEVTTVICDS 186

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2712 AHLG---IMNGSLP----VILLDDGETRPFDNEPD------------------TPLDARKQglTPRHLAYVIYTSGSTGK 2766
Cdd:PLN02387  187 KQLKkliDISSQLEtvkrVIYMDDEGVDSDSSLSGssnwtvssfseveklgkeNPVDPDLP--SPNDIAVIMYTSGSTGL 264

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 2767 PKGVMVEHANMVNFLCSMRKE-PGIAQEDVLLGVTSLSfdiSILEI 2811
Cdd:PLN02387  265 PKGVMMTHGNIVATVAGVMTVvPKLGKNDVYLAYLPLA---HILEL 307
PP-binding pfam00550
Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached ...
 986-1041 3.11e-09

Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. This family includes members not included in Prosite. The inclusion of these members is supported by sequence analysis and functional evidence. The related domain of Swiss:P19828 has the attachment serine replaced by an alanine.


Pssm-ID: 425746 [Multi-domain]  Cd Length: 62  Bit Score: 55.26  E-value: 3.11e-09
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967   986 ALAALWQELLGLD--RVGRHDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTL 1041
Cdd:pfam00550    2 RLRELLAEVLGVPaeEIDPDTDLFDLGLDSLLAVELIARLEEEfGVEIPPSDLFEHPTL 60
FATP_FACS cd05940
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ...
 2629-3032 3.32e-09

Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341263 [Multi-domain]  Cd Length: 449  Bit Score: 62.37  E-value: 3.32e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2629 EASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALI 2708
Cdd:cd05940     1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2709 sqsahlgimngslpvillddgetrpFDnepdtpldarkqgltprhLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEP 2788
Cdd:cd05940    81 -------------------------VD------------------AALYIYTSGTTGLPKAAIISHRRAWRGGAFFAGSG 117

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2789 GIAQEDVLLGVTSLSFDI-SILEIFLPLLNGARLILATQAQAADAQQLamlIERHAVSFMQATPSTWRMLV------ELR 2861
Cdd:cd05940   118 GALPSDVLYTCLPLYHSTaLIVGWSACLASGATLVIRKKFSASNFWDD---IRKYQATIFQYIGELCRYLLnqppkpTER 194

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2862 DFALppgfKALCGGEALP---ENLATALlqKVTTLWNLYGPTETTIwSTLN--------GLTTPTPYIGHPIA------N 2924
Cdd:cd05940   195 KHKV----RMIFGNGLRPdiwEEFKERF--GVPRIAEFYAATEGNS-GFINffgkpgaiGRNPSLLRKVAPLAlvkydlE 267

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2925 TQIYILDAQGRV--VPLGVAGEI--HIAGAGVVRGYLGrPDLTAERFITDPFSGAPeaRMYKTGDLGRWLPDGTLEYLGR 3000
Cdd:cd05940   268 SGEPIRDAEGRCikVPRGEPGLLisRINPLEPFDGYTD-PAATEKKILRDVFKKGD--AWFNTGDLMRLDGEGFWYFVDR 344

                         410       420       430
                  ....*....|....*....|....*....|....
gi 641744967 3001 --NDFQVKvrGFRIELGEIETRLARCHGVHDAVV 3032
Cdd:cd05940   345 lgDTFRWK--GENVSTTEVAAVLGAFPGVEEANV 376
PRK05851 PRK05851
long-chain-fatty acid--ACP ligase MbtM;
1562-2018 5.40e-09

long-chain-fatty acid--ACP ligase MbtM;


Pssm-ID: 180289 [Multi-domain]  Cd Length: 525  Bit Score: 61.71  E-value: 5.40e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1562 RANQLAHHLIDLGvkPDDRIAICVERSLDMVIGLLAILKAGAAyVPLDPG------------YPAERLA-----YMLDDA 1624
Cdd:PRK05851   40 RAENVAARLLDRD--RPGAVGLVGEPTVELVAAIQGAWLAGAA-VSILPGpvrgaddgrwadATLTRFAgigvrTVLSHG 116

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1625 SPVALLtQANQRALLTGDVPRIlldtadfSHLSEDNPhVPGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNT 1704
Cdd:PRK05851  117 SHLERL-RAVDSSVTVHDLATA-------AHTNRSAS-LTPPDSGGPAVLQGTAGSTGTPRTAILSPGAVLSNLRGLNAR 187

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1705 YRLTPD-DRVLQKTPFSFDVSVWEFFWPLLYGARLVMArPDGHKDAA---YLAQLIE-RTGITTL-HFVPSMLQQFVQWA 1778
Cdd:PRK05851  188 VGLDAAtDVGCSWLPLYHDMGLAFLLTAALAGAPLWLA-PTTAFSASpfrWLSWLSDsRATLTAApNFAYNLIGKYARRV 266

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1779 DaDCACDSLRRVICSGEALPAELQQRF---FARFN---AQLHNLYGPTEAAIDVTFWAC----QPDD--------HRSFV 1840
Cdd:PRK05851  267 S-DVDLGALRVALNGGEPVDCDGFERFataMAPFGfdaGAAAPSYGLAESTCAVTVPVPgiglRVDEvttddgsgARRHA 345

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1841 PIGRPIANTQLYILDTlGQPVPLGV--AGELHIGGVGVARGYLNRPDLTAERFIPdpfinqpgarlykTGDLArWLPDGS 1918
Cdd:PRK05851  346 VLGNPIPGMEVRISPG-DGAAGVAGreIGEIEIRGASMMSGYLGQAPIDPDDWFP-------------TGDLG-YLVDGG 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1919 LEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRlvaylcpqPGVT-------PDPADLRQQLGQ 1991
Cdd:PRK05851  411 LVVCGRAKELITVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGSAR--------PGLViaaefrgPDEAGARSEVVQ 482

                         490       500       510
                  ....*....|....*....|....*....|.
gi 641744967 1992 HLAEY--MVPG--AFVTLDAFPLTPNGKLDR 2018
Cdd:PRK05851  483 RVASEcgVVPSdvVFVAPGSLPRTSSGKLRR 513
AcpP COG0236
Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the ...
 2036-2110 9.36e-09

Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the Pathway/BioSystem: Fatty acid biosynthesis


Pssm-ID: 440006 [Multi-domain]  Cd Length: 80  Bit Score: 54.47  E-value: 9.36e-09
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967 2036 APQGEVETALAAVWQDLLGL--TRVGRHDHFFA-LGGHSLLIVSLIERLRRA-GLALDVRGVFSTPVLSDMAQAILAHQ 2110
Cdd:COG0236     1 MPREELEERLAEIIAEVLGVdpEEITPDDSFFEdLGLDSLDAVELIAALEEEfGIELPDTELFEYPTVADLADYLEEKL 79
PRK08043 PRK08043
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
1672-2017 1.18e-08

bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;


Pssm-ID: 181207 [Multi-domain]  Cd Length: 718  Bit Score: 60.88  E-value: 1.18e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1672 AYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPF--SFDVSVwEFFWPLLYGARLVMARPDGHkda 1749
Cdd:PRK08043  368 ALILFTSGSEGHPKGVVHSHKSLLANVEQIKTIADFTPNDRFMSALPLfhSFGLTV-GLFTPLLTGAEVFLYPSPLH--- 443

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1750 aY--LAQLIERTGITTLHFVPSMLQQFVQWADA-DCAcdSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEAA--- 1823
Cdd:PRK08043  444 -YriVPELVYDRNCTVLFGTSTFLGNYARFANPyDFA--RLRYVVAGAEKLQESTKQLWQDKFGLRILEGYGVTECApvv 520

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1824 -IDVTFwACQPDDhrsfvpIGRPIANTQLYILdtlgqPVPlGVA--GELHIGGVGVARGYL--NRPDL----TAErfipd 1894
Cdd:PRK08043  521 sINVPM-AAKPGT------VGRILPGMDARLL-----SVP-GIEqgGRLQLKGPNIMNGYLrvEKPGVlevpTAE----- 582

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1895 pfiNQPG---ARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGDTRLVAY 1971
Cdd:PRK08043  583 ---NARGemeRGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKQHATAIKSDASKGEALVLF 659

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|.
gi 641744967 1972 lcpqpgvTPDPADLRQQLGQH-----LAEYMVPGAFVTLDAFPLTPNGKLD 2017
Cdd:PRK08043  660 -------TTDSELTREKLQQYarehgVPELAVPRDIRYLKQLPLLGSGKPD 703
PRK06334 PRK06334
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
 1663-1977 1.23e-08

long chain fatty acid--[acyl-carrier-protein] ligase; Validated


Pssm-ID: 180533 [Multi-domain]  Cd Length: 539  Bit Score: 60.60  E-value: 1.23e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1663 VPGLDAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQNTYRLTPDDRVLQKTP----FSFDVSVwefFWPLLYGARL 1738
Cdd:PRK06334  177 VSDKDPEDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPpfhaYGFNSCT---LFPLLSGVPV 253

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1739 VMA-RPDGHKDaayLAQLIERTGITTLHFVPSMLQQFVQWADADCAC-DSLRRVICSGEALPAELQQRFFARF-NAQLHN 1815
Cdd:PRK06334  254 VFAyNPLYPKK---IVEMIDEAKVTFLGSTPVFFDYILKTAKKQESClPSLRFVVIGGDAFKDSLYQEALKTFpHIQLRQ 330

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1816 LYGPTEAAIDVTFWACQPDDHRSFVpiGRPIANTQLYIL-DTLGQPVPLGVAGELHIGGVGVARGYLNRpdltaerfipD 1894
Cdd:PRK06334  331 GYGTTECSPVITINTVNSPKHESCV--GMPIRGMDVLIVsEETKVPVSSGETGLVLTRGTSLFSGYLGE----------D 398

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1895 P---FINQPGARLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAvvvaredsPGDTRLVay 1971
Cdd:PRK06334  399 FgqgFVELGGETWYVTGDLGYVDRHGELFLKGRLSRFVKIGAEMVSLEALESILMEGFGQNAA--------DHAGPLV-- 468


                  ....*.
gi 641744967 1972 LCPQPG 1977
Cdd:PRK06334  469 VCGLPG 474
hsFATP2a_ACSVL_like cd05938
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ...
 494-640 1.33e-08

Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341261 [Multi-domain]  Cd Length: 537  Bit Score: 60.38  E-value: 1.33e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  494 FEDQHLTYRELNRRANQLAHHLIA-LGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPV 572
Cdd:cd05938     1 FEGETYTYRDVDRRSNQAARALLAhAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  573 ALLTQSAQVAQLNSTLPTVLLD-------------------TPAAAACPDTnPVVQGLHAA----HLAYVIYTSGSTGRP 629
Cdd:cd05938    81 VLVVAPELQEAVEEVLPALRADgvsvwylshtsntegvislLDKVDAASDE-PVPASLRAHvtikSPALYIYTSGTTGLP 159

                         170
                  ....*....|.
gi 641744967  630 KGVMVAHRNVI 640
Cdd:cd05938   160 KAARISHLRVL 170
PRK06334 PRK06334
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
 2748-3099 1.87e-08

long chain fatty acid--[acyl-carrier-protein] ligase; Validated


Pssm-ID: 180533 [Multi-domain]  Cd Length: 539  Bit Score: 60.21  E-value: 1.87e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2748 GLTPRHLAYVIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIAQEDVLLGVT----SLSFDISILeifLPLLNGARLIL 2823
Cdd:PRK06334  179 DKDPEDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLppfhAYGFNSCTL---FPLLSGVPVVF 255

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2824 ATQAQAADAQQLamLIERHAVSFMQATPSTWRMLVEL---RDFALPPGFKALCGGEALPENL--ATALLQKVTTLWNLYG 2898
Cdd:PRK06334  256 AYNPLYPKKIVE--MIDEAKVTFLGSTPVFFDYILKTakkQESCLPSLRFVVIGGDAFKDSLyqEALKTFPHIQLRQGYG 333

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2899 PTETTIWSTLNGLTTP--TPYIGHPIANTQIYILDAQGRV-VPLGVAGEIHIAGAGVVRGYLGrpdltaerfiTDPFSGA 2975
Cdd:PRK06334  334 TTECSPVITINTVNSPkhESCVGMPIRGMDVLIVSEETKVpVSSGETGLVLTRGTSLFSGYLG----------EDFGQGF 403

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2976 PE---ARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDA------VVIAredSPGDKRLVA 3046
Cdd:PRK06334  404 VElggETWYVTGDLGYVDRHGELFLKGRLSRFVKIGAEMVSLEALESILMEGFGQNAAdhagplVVCG---LPGEKVRLC 480

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|...
gi 641744967 3047 YLLAQPDTVLEPADLRQRLSegVAEYMIPSAFVTLDAFPLTPNGKLDRKALPA 3099
Cdd:PRK06334  481 LFTTFPTSISEVNDILKNSK--TSSILKISYHHQVESIPMLGTGKPDYCSLNA 531
PRK07868 PRK07868
acyl-CoA synthetase; Validated
 1532-2024 1.89e-08

acyl-CoA synthetase; Validated


Pssm-ID: 236121 [Multi-domain]  Cd Length: 994  Bit Score: 60.50  E-value: 1.89e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1532 QLVEDQAARTPDTTAVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPG 1611
Cdd:PRK07868  451 RIIAEQARDAPKGEFLLFDGRVHTYEAVNRRINNVVRGLIAVGVRQGDRVGVLMETRPSALVAIAALSRLGAVAVLMPPD 530

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1612 ---YPAERLA---YMLDDASPVALLTQANQRALLTG-------DVPrillDTADFSHLSEDNPHV----------PGLdA 1668
Cdd:PRK07868  531 tdlAAAVRLGgvtEIITDPTNLEAARQLPGRVLVLGggesrdlDLP----DDADVIDMEKIDPDAvelpgwyrpnPGL-A 605

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1669 HHLAYVIY-TSGSTGKPKGVMNSHRALcnRLVWMQNTYRLTPDDRVLQKTPFSFDVSvweffwpLLY--------GARLV 1739
Cdd:PRK07868  606 RDLAFIAFsTAGGELVAKQITNYRWAL--SAFGTASAAALDRRDTVYCLTPLHHESG-------LLVslggavvgGSRIA 676

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1740 MARpdgHKDAAYLAQLIERTGITTLHFVPSMLQQFVQwaDADCACD---SLRRVICSGeaLPAELQQRFFARFN-AQLHN 1815
Cdd:PRK07868  677 LSR---GLDPDRFVQEVRQYGVTVVSYTWAMLREVVD--DPAFVLHgnhPVRLFIGSG--MPTGLWERVVEAFApAHVVE 749

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1816 LYGPTEA-AIDVTFWACQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGGVGVARGYLNRP-DLTAErFIP 1893
Cdd:PRK07868  750 FFATTDGqAVLANVSGAKIGSKGRPLPGAGRVELAAYDPEHDLILEDDRGFVRRAEVNEVGVLLARARGPiDPTAS-VKR 828

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1894 DPFinQPGARLYKTGDLARWLPDGSLEYLGRNDFQVK-LRGFrIELGEIEARLMQCPGVQEAVVVArEDSPGDTRLVAYL 1972
Cdd:PRK07868  829 GVF--APADTWISTEYLFRRDDDGDYWLVDRRGSVIRtARGP-VYTEPVTDALGRIGGVDLAVTYG-VEVGGRQLAVAAV 904

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 1973 CPQPGVTPDPADLRQQLGQhLAEYMVPGAFVTLDAFPLT----PN-GKLDRKALPAP 2024
Cdd:PRK07868  905 TLRPGAAITAADLTEALAS-LPVGLGPDIVHVVPEIPLSatyrPTvSALRAAGIPKP 960
ACSBG_like cd05933
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ...
 2632-3043 1.99e-08

Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341256 [Multi-domain]  Cd Length: 596  Bit Score: 60.06  E-value: 1.99e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALI--- 2708
Cdd:cd05933     9 LTYKEYYEACRQAAKAFLKLGLERFHGVGILGFNSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSEANILVven 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2709 -SQSAHLGIMNGSLP----VILLDD--GETRP-------F----DNEPDTPLDARKQGLTPRHLAYVIYTSGSTGKPKGV 2770
Cdd:cd05933    89 qKQLQKILQIQDKLPhlkaIIQYKEplKEKEPnlyswdeFmelgRSIPDEQLDAIISSQKPNQCCTLIYTSGTTGMPKGV 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2771 MVEHANMV----NFLCSMRKEPGIAQEDVLLGVTSLSF-DISILEIFLPLLNGARLILATQAQAADAQQLAM-------- 2837
Cdd:cd05933   169 MLSHDNITwtakAASQHMDLRPATVGQESVVSYLPLSHiAAQILDIWLPIKVGGQVYFAQPDALKGTLVKTLrevrptaf 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2838 ---------LIERHAVSFMQATPSTWRMLVELRDFAL------------PPGFKAL-----------------C-----G 2874
Cdd:cd05933   249 mgvprvwekIQEKMKAVGAKSGTLKRKIASWAKGVGLetnlklmggespSPLFYRLakklvfkkvrkalgldrCqkfftG 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2875 GEALPENLATALLQKVTTLWNLYGPTETTIWSTLNGLTTPTPY-IGHPIANTQIYIL--DAQGrvvplgvAGEIHIAGAG 2951
Cdd:cd05933   329 AAPISRETLEFFLSLNIPIMELYGMSETSGPHTISNPQAYRLLsCGKALPGCKTKIHnpDADG-------IGEICFWGRH 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2952 VVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQVKVRGfrielGE------IETRL-ARC 3024
Cdd:cd05933   402 VFMGYLNMEDKTEEAIDEDGW--------LHSGDLGKLDEDGFLYITGRIKELIITAG-----GEnvppvpIEDAVkKEL 468

                         490
                  ....*....|....*....
gi 641744967 3025 HGVHDAVVIaredspGDKR 3043
Cdd:cd05933   469 PIISNAMLI------GDKR 481
LCL_NRPS-like cd19540
LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; ...
 30-316 2.22e-08

LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380463 [Multi-domain]  Cd Length: 433  Bit Score: 59.36  E-value: 2.22e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEKGDTYllNSLLAFdsqtRLDAFLDV------LQQVIARHDILRTaICWQGLHQPVQVV----W 99
Cdd:cd19540     3 PLSFAQQRLWFLNRLDGPSAAY--NIPLAL----RLTGALDVdalraaLADVVARHESLRT-VFPEDDGGPYQVVlpaaE 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  100 RQAPLTVntlTTTSSDTVPAQLRAATDpsnHRLNLSNAPLLSAT---TAHDpvcgEWLLSLSIHHLISDHitqaliiDEI 176
Cdd:cd19540    76 ARPDLTV---VDVTEDELAARLAEAAR---RGFDLTAELPLRARlfrLGPD----EHVLVLVVHHIAADG-------WSM 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  177 RLLLED------------RPEALPKPLPYRNFIA---QIL------SVPLSEHEQYFRNRLADIDTPTA-PFDLV--DVQ 232
Cdd:cd19540   139 APLARDlatayaarragrAPDWAPLPVQYADYALwqrELLgdeddpDSLAARQLAYWRETLAGLPEELElPTDRPrpAVA 218

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  233 GNGEDITEarLSLDSSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSGRLQGNLgaDRVMGMFINTL 312
Cdd:cd19540   219 SYRGGTVE--FTIDAELHARLAALAREHGATLFMVLHAALAVLLSRLGAGDDIPIGTPVAGRGDEAL--DDLVGMFVNTL 294


                  ....
gi 641744967  313 PLRV 316
Cdd:cd19540   295 VLRT 298
PTZ00237 PTZ00237
acetyl-CoA synthetase; Provisional
 1554-2021 2.69e-08

acetyl-CoA synthetase; Provisional


Pssm-ID: 240325 [Multi-domain]  Cd Length: 647  Bit Score: 59.76  E-value: 2.69e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDASPVALLTQ- 1632
Cdd:PTZ00237   93 LTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSLIDRIETITPKLIITTn 172

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1633 --------------------------------------ANQRALLTGDVPRI--LLDTAD-FSHLSEDN-----PHVPgL 1666
Cdd:PTZ00237  173 ygilndeiitftpnlkeaielstfkpsnvitlfrnditSESDLKKIETIPTIpnTLSWYDeIKKIKENNqspfyEYVP-V 251

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1667 DAHHLAYVIYTSGSTGKPKGVM--NSHRALCNRLVWmqnTYRLTPDD--RVLQKTPFSFdVSVWEFFWPLL-YGARLVMA 1741
Cdd:PTZ00237  252 ESSHPLYILYTSGTTGNSKAVVrsNGPHLVGLKYYW---RSIIEKDIptVVFSHSSIGW-VSFHGFLYGSLsLGNTFVMF 327

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1742 RPDGHKDAAY---LAQLIERTGITTLHFVPSMLQQFVQwADADCA-------CDSLRRVICSGEALPAELQQRFFARFNA 1811
Cdd:PTZ00237  328 EGGIIKNKHIeddLWNTIEKHKVTHTLTLPKTIRYLIK-TDPEATiirskydLSNLKEIWCGGEVIEESIPEYIENKLKI 406

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1812 QLHNLYGPTEaaIDVTFWACQPDDHRSFVPIGRPIANTQLYILDTLGQPVPLGVAGELHIGgvgvargyLNRPDLTAERF 1891
Cdd:PTZ00237  407 KSSRGYGQTE--IGITYLYCYGHINIPYNATGVPSIFIKPSILSEDGKELNVNEIGEVAFK--------LPMPPSFATTF 476

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1892 IPDP------FINQPGarLYKTGDLARWLPDGSLEYLGRNDFQVKLRGFRIELGEIEARLMQCPGVQEAVVVAREDSPGD 1965
Cdd:PTZ00237  477 YKNDekfkqlFSKFPG--YYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDCY 554

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967 1966 TRLVAYLC---PQPGVTPDPADLRQQLGQHLAEYMVPGAFVT----LDAFPLTPNGKLDRKAL 2021
Cdd:PTZ00237  555 NVPIGLLVlkqDQSNQSIDLNKLKNEINNIITQDIESLAVLRkiiiVNQLPKTKTGKIPRQII 617
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
 2919-3098 2.87e-08

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 59.24  E-value: 2.87e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2919 GHPIANTQIYILDAQgrvvplgvAGEIHIAGAGVVRGYLgrPDLTAERfitdpfsgapeaRMYKTGDLGRWLPDGTLEYL 2998
Cdd:PRK07445  286 GQVLPHAQITIPANQ--------TGNITIQAQSLALGYY--PQILDSQ------------GIFETDDLGYLDAQGYLHIL 343

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2999 GRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKRLVA-YLLAQPDTVLEpaDLRQRLSEGVAEYMIPSA 3077
Cdd:PRK07445  344 GRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAiYVPKDPSISLE--ELKTAIKDQLSPFKQPKH 421

                         170       180
                  ....*....|....*....|.
gi 641744967 3078 FVTLDAFPLTPNGKLDRKALP 3098
Cdd:PRK07445  422 WIPVPQLPRNPQGKINRQQLQ 442
PRK05851 PRK05851
long-chain-fatty acid--ACP ligase MbtM;
503-960 3.13e-08

long-chain-fatty acid--ACP ligase MbtM;


Pssm-ID: 180289 [Multi-domain]  Cd Length: 525  Bit Score: 59.39  E-value: 3.13e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  503 ELNRRANQLAHHLIALGvqPDDRVALCVERSLEMMVGLLGILKAGAAYV----PMDPAYPAERLAYILDDAAPVA---LL 575
Cdd:PRK05851   36 EVHGRAENVAARLLDRD--RPGAVGLVGEPTVELVAAIQGAWLAGAAVSilpgPVRGADDGRWADATLTRFAGIGvrtVL 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  576 TQSAQVAQLNSTLPTVLLDTPAAAACPDTNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHP 655
Cdd:PRK05851  114 SHGSHLERLRAVDSSVTVHDLATAAHTNRSASLTPPDSGGPAVLQGTAGSTGTPRTAILSPGAVLSNLRGLNARVGLDAA 193

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  656 SRIA--------------------------LNASIVFDASVKNWIQLL--SGHTLVLVPD---------ALRADAHQLWR 698
Cdd:PRK05851  194 TDVGcswlplyhdmglaflltaalagaplwLAPTTAFSASPFRWLSWLsdSRATLTAAPNfaynligkyARRVSDVDLGA 273

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  699 Y-FARHAVDLFDCTPVQLQWLLDAGLGSDPAyqpaqvliggeAISPAvwsrlqslsdtrfinvYGPTE--CTVDATAC-- 773
Cdd:PRK05851  274 LrVALNGGEPVDCDGFERFATAMAPFGFDAG-----------AAAPS----------------YGLAEstCAVTVPVPgi 326

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  774 --VVDR--------TQPLPTIGKPLANTRLYIlDAQDQPVPIGV--TGELHIGGAGVARGYLHRPDLTAERFIPdpfsad 841
Cdd:PRK05851  327 glRVDEvttddgsgARRHAVLGNPIPGMEVRI-SPGDGAAGVAGreIGEIEIRGASMMSGYLGQAPIDPDDWFP------ 399

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  842 paariykTGDLArWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTR---LVAYLCARP 918
Cdd:PRK05851  400 -------TGDLG-YLVDGGLVVCGRAKELITVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGSARpglVIAAEFRGP 471

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 641744967  919 DAELHPAALRQQLAASLAdyMIPS--AFVTLDALPLTPNGKLDR 960
Cdd:PRK05851  472 DEAGARSEVVQRVASECG--VVPSdvVFVAPGSLPRTSSGKLRR 513
C_NRPS-like cd19537
Condensation family domain with an atypical active site motif; Condensation (C) domains of ...
 2192-2449 3.55e-08

Condensation family domain with an atypical active site motif; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily typically have a non-canonical conserved SHXXXDX(14)Y motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380460 [Multi-domain]  Cd Length: 395  Bit Score: 58.74  E-value: 3.55e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2192 FTHRERLDAFLSALQQVIDRHDILRT-------AVCWQDLSQPVQVVWRQAIlpinhfeptspeDVLAQLQahteprtRR 2264
Cdd:cd19537    32 LSGDVDRDRLASAWNTVLARHRILRSryvprdgGLRRSYSSSPPRVQRVDTL------------DVWKEIN-------RP 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2265 IDLSQAPLFRADIAHDplqnewLLALSFHHLISDHMTLALIVGEIRLLLQHqadaLPTPLPYRNFIAQTL--SVPNSAHE 2342
Cdd:cd19537    93 FDLEREDPIRVFISPD------TLLVVMSHIICDLTTLQLLLREVSAAYNG----KLLPPVRREYLDSTAwsRPASPEDL 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2343 AYFRDKLADVDEPTAPfGLLNVQGSGGDIHEARLvlDATLASAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGS 2422
Cdd:cd19537   163 DFWSEYLSGLPLLNLP-RRTSSKSYRGTSRVFQL--PGSLYRSLLQFSTSSGITLHQLALAAVALALQDLSDRTDIVLGA 239

                         250       260
                  ....*....|....*....|....*..
gi 641744967 2423 VLLGRlaGAEGADRIMGMFINTLPLRI 2449
Cdd:cd19537   240 PYLNR--TSEEDMETVGLFLEPLPIRI 264
PTZ00216 PTZ00216
acyl-CoA synthetase; Provisional
 430-649 3.67e-08

acyl-CoA synthetase; Provisional


Pssm-ID: 240316 [Multi-domain]  Cd Length: 700  Bit Score: 59.22  E-value: 3.67e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  430 ENLVTSLEKT----PQQPALSQPILPKSERQQVlvdfnaTDAD-FPREMliqqrfeaqaeqtpeAIAVLFEDQHLTYREL 504
Cdd:PTZ00216   69 PNFLQRLERIckerGDRRALAYRPVERVEKEVV------KDADgKERTM---------------EVTHFNETRYITYAEL 127

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  505 NRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQSAQVAQL 584
Cdd:PTZ00216  128 WERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAIVCNGKNVPNL 207

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  585 ----------------NSTLPT---------------VLLDTPAAAACPDTNPvvqgLHAAHLAYVIYTSGSTGRPKGVM 633
Cdd:PTZ00216  208 lrlmksggmpnttiiyLDSLPAsvdtegcrlvawtdvVAKGHSAGSHHPLNIP----ENNDDLALIMYTSGTTGDPKGVM 283

                         250
                  ....*....|....*.
gi 641744967  634 VAHRnviNLATGLHTL 649
Cdd:PTZ00216  284 HTHG---SLTAGILAL 296
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
 2624-3096 3.86e-08

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 59.24  E-value: 3.86e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2624 AVVFGEAS----LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPL-DPTYPVERLRYM 2698
Cdd:PRK07768   18 GMVTGEPDapvrHTWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIAPTAQGLWMRGASLTMLhQPTPRTDLAVWA 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2699 LDDAKPVALISQSAhlgimngslpVILLDdgetrPFD----------------NEPDTPLDARKQGLTPRHLAYVIYTSG 2762
Cdd:PRK07768   98 EDTLRVIGMIGAKA----------VVVGE-----PFLaaapvleekgirvltvADLLAADPIDPVETGEDDLALMQLTSG 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2763 STGKPKGVMVEHANMVNFLCSMRKEPGIAQE-DVLLGVTSLSFDISILEIF-LPLLNGARLILATQAQAADAQQLAM-LI 2839
Cdd:PRK07768  163 STGSPKAVQITHGNLYANAEAMFVAAEFDVEtDVMVSWLPLFHDMGMVGFLtVPMYFGAELVKVTPMDFLRDPLLWAeLI 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2840 ERHAVSFMQATPSTWRMLVE-LRDFALPPGFK------ALCGGE----ALPENLATALLQ---KVTTLWNLYGPTETTIW 2905
Cdd:PRK07768  243 SKYRGTMTAAPNFAYALLARrLRRQAKPGAFDlsslrfALNGAEpidpADVEDLLDAGARfglRPEAILPAYGMAEATLA 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2906 STL----NGLTTPTP-----------------------YIGHPIANTQIYILDAQGRVVPLGVAGEIHIAGAGVVRGYLg 2958
Cdd:PRK07768  323 VSFspcgAGLVVDEVdadllaalrravpatkgntrrlaTLGPPLPGLEVRVVDEDGQVLPPRGVGVIELRGESVTPGYL- 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2959 rpdltaerfITDPFSGAPEAR-MYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIARED 3037
Cdd:PRK07768  402 ---------TMDGFIPAQDADgWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAAARVEGVRPGNAVAVRL 472

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 3038 SPGDKRLVAYLLAQPDTVLEPADLRqRLSEGVAEY------MIPSAFVTLDA--FPLTPNGKLDRKA 3096
Cdd:PRK07768  473 DAGHSREGFAVAVESNAFEDPAEVR-RIRHQVAHEvvaevgVRPRNVVVLGPgsIPKTPSGKLRRAN 538
PRK06814 PRK06814
acyl-[ACP]--phospholipid O-acyltransferase;
617-959 4.43e-08

acyl-[ACP]--phospholipid O-acyltransferase;


Pssm-ID: 235865 [Multi-domain]  Cd Length: 1140  Bit Score: 59.21  E-value: 4.43e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  617 AYVIYTSGSTGRPKGVMVAHRNVinLATGLHTLLALD-HPSRIALNASIVFDA---SVKNWIQLLSGHTLVLVPDALR-- 690
Cdd:PRK06814  796 AVILFTSGSEGTPKGVVLSHRNL--LANRAQVAARIDfSPEDKVFNALPVFHSfglTGGLVLPLLSGVKVFLYPSPLHyr 873

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  691 --------ADAHQLWRY------FAR--HAVDLfdctpvqlqwlldaglgsdpaYQPAQVLIGGEAISPA---VWSRLQS 751
Cdd:PRK06814  874 iipeliydTNATILFGTdtflngYARyaHPYDF---------------------RSLRYVFAGAEKVKEEtrqTWMEKFG 932

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  752 LsdtRFINVYGPTEctvdaTACVVDRTQPL----PTIGK--PLANTRLyildaqdQPVP-IGVTGELHIGGAGVARGYLH 824
Cdd:PRK06814  933 I---RILEGYGVTE-----TAPVIALNTPMhnkaGTVGRllPGIEYRL-------EPVPgIDEGGRLFVRGPNVMLGYLR 997

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  825 rpdltAERfipdPFSADPAARI-YKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIE---SRLLrcPGVQDAVViA 900
Cdd:PRK06814  998 -----AEN----PGVLEPPADGwYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEelaAELW--PDALHAAV-S 1065

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 641744967  901 REDSPGDTRLVaYLCARPDAElHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLD 959
Cdd:PRK06814 1066 IPDARKGERII-LLTTASDAT-RAAFLAHAKAAGASELMVPAEIITIDEIPLLGTGKID 1122
E_NRPS cd19534
Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 2190-2463 8.01e-08

Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Epimerization (E) domains of nonribosomal peptide synthetases (NRPS) flip the chirality of the end amino acid of a peptide being manufactured by the NRPS. E-domains are homologous to the Condensation (C) domains. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Specialized tailoring NRPS domains such as E-domains greatly increase the range of possible peptide products created by the NRPS machinery. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the E-domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380457 [Multi-domain]  Cd Length: 428  Bit Score: 57.65  E-value: 8.01e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2190 VAFTHRERLD--AFLSALQQVIDRHDILRtAVCWQDlsqpvQVVWRQAILPINHFEPT------SPEDVLAQLQAHTEPR 2261
Cdd:cd19534    26 VLLRVPQGLDpdALRQALRALVEHHDALR-MRFRRE-----DGGWQQRIRGDVEELFRlevvdlSSLAQAAAIEALAAEA 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2262 TRRIDLSQAPLFRADIAHDPlQNEWLLALSFHHLISDHMTLALIVGEIRLLLQHQADALPTPLP----YRNFI---AQTL 2334
Cdd:cd19534   100 QSSLDLEEGPLLAAALFDGT-DGGDRLLLVIHHLVVDGVSWRILLEDLEAAYEQALAGEPIPLPsktsFQTWAellAEYA 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2335 SVPNSAHEAYFRDKLadvdEPTAPFGL-LNVQGSGGDIHEARLVLDATLASAIRQQA-RHLGVSPGVLFHVAWAQVLAQT 2412
Cdd:cd19534   179 QSPALLEELAYWREL----PAADYWGLpKDPEQTYGDARTVSFTLDEEETEALLQEAnAAYRTEINDLLLAALALAFQDW 254

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 641744967 2413 SGRDDVVfgsVLL---GRLAGAEGAD--RIMGMFINTLPLRISLADRGA-AEVVERT 2463
Cdd:cd19534   255 TGRAPPA---IFLeghGREEIDPGLDlsRTVGWFTSMYPVVLDLEASEDlGDTLKRV 308
C_NRPS-like cd19537
Condensation family domain with an atypical active site motif; Condensation (C) domains of ...
 30-330 9.86e-08

Condensation family domain with an atypical active site motif; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily typically have a non-canonical conserved SHXXXDX(14)Y motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380460 [Multi-domain]  Cd Length: 395  Bit Score: 57.20  E-value: 9.86e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   30 PLAPLQEGILFHYQLQEKGDTYLLNSLLAFDSQTRLDAFLDVLQQVIARHDILRTAICwQGLHQPVQVVWRQAPlTVNTL 109
Cdd:cd19537     3 ALSPIEREWWHKYQLSTGTSSFNVSFACRLSGDVDRDRLASAWNTVLARHRILRSRYV-PRDGGLRRSYSSSPP-RVQRV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  110 TTTSsdtvpaqLRAAtdpSNHRLNLSNAPLLSATTAHDpvcgewLLSLSIHHLISDHITQALIIDEI------RLLLEDR 183
Cdd:cd19537    81 DTLD-------VWKE---INRPFDLEREDPIRVFISPD------TLLVVMSHIICDLTTLQLLLREVsaayngKLLPPVR 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  184 PEALPKPLPYRnfiaqilsVPLSEHEQYFRNRLADIDTPTAPFDLVDVQGNGEDITearLSLDSSLADALRRQARHLGIS 263
Cdd:cd19537   145 REYLDSTAWSR--------PASPEDLDFWSEYLSGLPLLNLPRRTSSKSYRGTSRV---FQLPGSLYRSLLQFSTSSGIT 213

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 641744967  264 ssvLFHVAWAQV-LAL--TSGRDDVVFGSVLSGRlqGNLGADRVMGMFINTLPLRVSL---RERSVHDVVQAT 330
Cdd:cd19537   214 ---LHQLALAAVaLALqdLSDRTDIVLGAPYLNR--TSEEDMETVGLFLEPLPIRIRFpssSDASAADFLRAV 281
PLN02614 PLN02614
long-chain acyl-CoA synthetase
 619-882 1.30e-07

long-chain acyl-CoA synthetase


Pssm-ID: 166255 [Multi-domain]  Cd Length: 666  Bit Score: 57.72  E-value: 1.30e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  619 VIYTSGSTGRPKGVMVAHRNVINLATGLHTLLALDHPSRIALNASI-------VFD-----------ASVKNW---IQLL 677
Cdd:PLN02614  228 IMYTSGTTGDPKGVMISNESIVTLIAGVIRLLKSANAALTVKDVYLsylplahIFDrvieecfiqhgAAIGFWrgdVKLL 307

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  678 SGHTLVLVPDALRADAHQLWRYFARHAVDL----------FDC-------------TPVQLQWLLDA--------GLGSD 726
Cdd:PLN02614  308 IEDLGELKPTIFCAVPRVLDRVYSGLQKKLsdggflkkfvFDSafsykfgnmkkgqSHVEASPLCDKlvfnkvkqGLGGN 387

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  727 payqpAQVLIGGEA-ISPAVWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANtrlyiLDAQDQPVP- 804
Cdd:PLN02614  388 -----VRIILSGAApLASHVESFLRVVACCHVLQGYGLTESCAGTFVSLPDELDMLGTVGPPVPN-----VDIRLESVPe 457

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  805 -----IGVT--GELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPDGNIDYLGRndfqiKVRGFR 877
Cdd:PLN02614  458 meydaLASTprGEICIRGKTLFSGYYKREDLTKEVLIDGWL---------HTGDVGEWQPNGSMKIIDR-----KKNIFK 523


                  ....*
gi 641744967  878 IEAGE 882
Cdd:PLN02614  524 LSQGE 528
PRK05851 PRK05851
long-chain-fatty acid--ACP ligase MbtM;
2918-3094 1.48e-07

long-chain-fatty acid--ACP ligase MbtM;


Pssm-ID: 180289 [Multi-domain]  Cd Length: 525  Bit Score: 57.08  E-value: 1.48e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 IGHPIANTQIYIL--DAQGRVVPLGVaGEIHIAGAGVVRGYLGRPDLTAERFitdpfsgapearmYKTGDLGrWLPDGTL 2995
Cdd:PRK05851  347 LGNPIPGMEVRISpgDGAAGVAGREI-GEIEIRGASMMSGYLGQAPIDPDDW-------------FPTGDLG-YLVDGGL 411

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2996 EYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVIAREDSPGDKR---LVAYLLAQPDTVLEPADLRQRLSE--GVa 3070
Cdd:PRK05851  412 VVCGRAKELITVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGSARpglVIAAEFRGPDEAGARSEVVQRVASecGV- 490

                         170       180
                  ....*....|....*....|....*.
gi 641744967 3071 eymIPS--AFVTLDAFPLTPNGKLDR 3094
Cdd:PRK05851  491 ---VPSdvVFVAPGSLPRTSSGKLRR 513
PLN02614 PLN02614
long-chain acyl-CoA synthetase
 1674-1943 2.20e-07

long-chain acyl-CoA synthetase


Pssm-ID: 166255 [Multi-domain]  Cd Length: 666  Bit Score: 56.95  E-value: 2.20e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1674 VIYTSGSTGKPKGVMNSHRALCN------RLVWMQNTyRLTPDDRVLQKTPFS--FDVSVWEFFwpLLYGARLVMARPDG 1745
Cdd:PLN02614  228 IMYTSGTTGDPKGVMISNESIVTliagviRLLKSANA-ALTVKDVYLSYLPLAhiFDRVIEECF--IQHGAAIGFWRGDV 304

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1746 H---KDAAYLAQLIERTGITTLHFVPSMLQQ------FVQWADADCA--------------------CDSLR-------- 1788
Cdd:PLN02614  305 KlliEDLGELKPTIFCAVPRVLDRVYSGLQKklsdggFLKKFVFDSAfsykfgnmkkgqshveasplCDKLVfnkvkqgl 384

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1789 ----RVICSGEAlPAELQQRFFARFNAQLHNL--YGPTEAAIDvTFwACQPDDHRSFVPIGRPIANTQLYI-------LD 1855
Cdd:PLN02614  385 ggnvRIILSGAA-PLASHVESFLRVVACCHVLqgYGLTESCAG-TF-VSLPDELDMLGTVGPPVPNVDIRLesvpemeYD 461

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1856 TLGQPVplgvAGELHIGGVGVARGYLNRPDLTAERFIpDPFINqpgarlykTGDLARWLPDGSLEYLGRndfqvKLRGFR 1935
Cdd:PLN02614  462 ALASTP----RGEICIRGKTLFSGYYKREDLTKEVLI-DGWLH--------TGDVGEWQPNGSMKIIDR-----KKNIFK 523


                  ....*...
gi 641744967 1936 IELGEIEA 1943
Cdd:PLN02614  524 LSQGEYVA 531
AFD_CAR-like cd17632
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ...
 2630-3092 2.30e-07

adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.


Pssm-ID: 341287 [Multi-domain]  Cd Length: 588  Bit Score: 56.69  E-value: 2.30e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2630 ASLSYDELNRRANRLAH-HLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALI 2708
Cdd:cd17632    66 ETITYAELWERVGAVAAaHDPEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRLLA 145

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2709 SQSAHLgimngSLPVILLDDGETRP----FDNEPD-------------------------TPLDARKQGLTPRH------ 2753
Cdd:cd17632   146 VSAEHL-----DLAVEAVLEGGTPPrlvvFDHRPEvdahraalesarerlaavgipvttlTLIAVRGRDLPPAPlfrpep 220

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2754 ----LAYVIYTSGSTGKPKGVMVEHANMVNF-LCSMRKEPGIAQEDVLLGVTSLSF-----------------------D 2805
Cdd:cd17632   221 dddpLALLIYTSGSTGTPKGAMYTERLVATFwLKVSSIQDIRPPASITLNFMPMSHiagrislygtlarggtayfaaasD 300

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2806 ISILEIFLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQATPSTWRMLVELRDFALPPGFK-ALCGGEALPENLAT 2884
Cdd:cd17632   301 MSTLFDDLALVRPTELFLVPRVCDMLFQRYQAELDRRSVAGADAETLAERVKAELRERVLGGRLLaAVCGSAPLSAEMKA 380

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2885 ---ALLQkvTTLWNLYGPTETTIwSTLNGLTTPTPYIGHPIANtqiyildaqgrvVP-LGV--------AGEIHIAGAGV 2952
Cdd:cd17632   381 fmeSLLD--LDLHDGYGSTEAGA-VILDGVIVRPPVLDYKLVD------------VPeLGYfrtdrphpRGELLVKTDTL 445

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2953 VRGYLGRPDLTAERFITDPFsgapearmYKTGD-LGRWLPDgTLEYLGRNDFQVKV-RGFRIELGEIETRLARCHGVHDA 3030
Cdd:cd17632   446 FPGYYKRPEVTAEVFDEDGF--------YRTGDvMAELGPD-RLVYVDRRNNVLKLsQGEFVTVARLEAVFAASPLVRQI 516

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 3031 VVIAREDSpgdkrlvAYLLA------QPDTVLEPADLRQRLSE---------GVAEYMIPSAFVtLDAFPLTP-NGKL 3092
Cdd:cd17632   517 FVYGNSER-------AYLLAvvvptqDALAGEDTARLRAALAEslqriareaGLQSYEIPRDFL-IETEPFTIaNGLL 586
PLN02479 PLN02479
acetate-CoA ligase
 2759-3099 3.06e-07

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 56.01  E-value: 3.06e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2759 YTSGSTGKPKGVMVEH--ANMVNFLCSMrkepgiaqedvLLGVTSLSFDISILEIF----------LPLLNGARLILATQ 2826
Cdd:PLN02479  202 YTSGTTASPKGVVLHHrgAYLMALSNAL-----------IWGMNEGAVYLWTLPMFhcngwcftwtLAALCGTNICLRQV 270

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2827 AQAADAQQlamlIERHAVSFMQATPSTWRMLV----ELRDFALPPGFKALCGGEALPENLATALLQK---VTTLWNL--- 2896
Cdd:PLN02479  271 TAKAIYSA----IANYGVTHFCAAPVVLNTIVnapkSETILPLPRVVHVMTAGAAPPPSVLFAMSEKgfrVTHTYGLset 346

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2897 YGPTETTIWST-----------------------LNGLTTPTPYIGHPIAntqiyildAQGRVVplgvaGEIHIAGAGVV 2953
Cdd:PLN02479  347 YGPSTVCAWKPewdslppeeqarlnarqgvryigLEGLDVVDTKTMKPVP--------ADGKTM-----GEIVMRGNMVM 413

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2954 RGYLGRPDLTAERFitdpfsgapEARMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHGVHDAVVI 3033
Cdd:PLN02479  414 KGYLKNPKANEEAF---------ANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVV 484

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967 3034 AREDSPGDKRLVAYLLAQP--DTVLEPA---DLRQRLSEGVAEYMIPSAfVTLDAFPLTPNGKLDRKALPA 3099
Cdd:PLN02479  485 ARPDERWGESPCAFVTLKPgvDKSDEAAlaeDIMKFCRERLPAYWVPKS-VVFGPLPKTATGKIQKHVLRA 554
Dip2 cd05905
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of ...
 495-721 1.14e-06

Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of the adenylate forming enzyme family, including insect luciferase, acetyl CoA ligases and the adenylation domain of nonribosomal peptide synthetases (NRPS). However, its function may have diverged from other members of the superfamily. In mouse embryo, Dip2 homolog A plays an important role in the development of both vertebrate and invertebrate nervous systems. Dip2A appears to regulate cell growth and the arrangement of cells in organs. Biochemically, Dip2A functions as a receptor of FSTL1, an extracellular glycoprotein, and may play a role as a cardiovascular protective agent.


Pssm-ID: 341231 [Multi-domain]  Cd Length: 571  Bit Score: 54.28  E-value: 1.14e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  495 EDQHLTYRELNRRANQLAHHLI-ALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDD----- 568
Cdd:cd05905    11 EATTLTWGKLLSRAEKIAAVLQkKVGLKPGDRVALMYPDPLDFVAAFYGCLYAGVVPIPIEPPDISQQLGFLLGTckvrv 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  569 ---------AAPVALLTQSAQVAQ-LNSTLP--TVLLDTPAAAACPDTNPVVQGLHAAH-LAYVIYTSGSTGRPKGVMVA 635
Cdd:cd05905    91 altveaclkGLPKKLLKSKTAAEIaKKKGWPkiLDFVKIPKSKRSKLKKWGPHPPTRDGdTAYIEYSFSSDGSLSGVAVS 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  636 HRNVINLATGLHTLLALDHPSRIALNASIVFDASVKNWIQL--LSGHTLVLVPDALRADAHQLW-----RYFARhavDLF 708
Cdd:cd05905   171 HSSLLAHCRALKEACELYESRPLVTVLDFKSGLGLWHGCLLsvYSGHHTILIPPELMKTNPLLWlqtlsQYKVR---DAY 247

                         250
                  ....*....|...
gi 641744967  709 dCTPVQLQWLLDA 721
Cdd:cd05905   248 -VKLRTLHWCLKD 259
PTZ00342 PTZ00342
acyl-CoA synthetase; Provisional
 1667-1937 1.32e-06

acyl-CoA synthetase; Provisional


Pssm-ID: 240370 [Multi-domain]  Cd Length: 746  Bit Score: 54.34  E-value: 1.32e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1667 DAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWMQN-----TYRLtpddrvlqKTPFSF-DVS-VWE---FFWPLLYGA 1736
Cdd:PTZ00342  302 DPDFITSIVYTSGTSGKPKGVMLSNKNLYNTVVPLCKhsifkKYNP--------KTHLSYlPIShIYErviAYLSFMLGG 373

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1737 RLVMARpdghKDAAYLAQLIERTGITTLHFVP----------------------SMLQQ------------FVQWADA-- 1780
Cdd:PTZ00342  374 TINIWS----KDINYFSKDIYNSKGNILAGVPkvfnriytnimteinnlpplkrFLVKKilslrksnnnggFSKFLEGit 449

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1781 -------DCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTEA--AIDVtfwacQPDDHRSFVPIGRPIANTQL 1851
Cdd:PTZ00342  450 hisskikDKVNPNLEVILNGGGKLSPKIAEELSVLLNVNYYQGYGLTETtgPIFV-----QHADDNNTESIGGPISPNTK 524

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1852 YIL---------DTLGQpvplgvaGELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlYKTGDLARWLPDGSLEYL 1922
Cdd:PTZ00342  525 YKVrtwetykatDTLPK-------GELLIKSDSIFSGYFLEKEQTKNAFTEDGY--------FKTGDIVQINKNGSLTFL 589

                         330
                  ....*....|....*.
gi 641744967 1923 GRNDFQVKL-RGFRIE 1937
Cdd:PTZ00342  590 DRSKGLVKLsQGEYIE 605
Dip2 cd05905
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of ...
 1550-1733 1.40e-06

Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of the adenylate forming enzyme family, including insect luciferase, acetyl CoA ligases and the adenylation domain of nonribosomal peptide synthetases (NRPS). However, its function may have diverged from other members of the superfamily. In mouse embryo, Dip2 homolog A plays an important role in the development of both vertebrate and invertebrate nervous systems. Dip2A appears to regulate cell growth and the arrangement of cells in organs. Biochemically, Dip2A functions as a receptor of FSTL1, an extracellular glycoprotein, and may play a role as a cardiovascular protective agent.


Pssm-ID: 341231 [Multi-domain]  Cd Length: 571  Bit Score: 53.89  E-value: 1.40e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1550 EDQHLTYDALNRRANQLAHHLID-LGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYML-----DD 1623
Cdd:cd05905    11 EATTLTWGKLLSRAEKIAAVLQKkVGLKPGDRVALMYPDPLDFVAAFYGCLYAGVVPIPIEPPDISQQLGFLLgtckvRV 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1624 ASPVALLTQANQRALLTGD----------VPRILldtaDFSHLSEDNPHV-------PGLDAHHLAYVIYTSGSTGKPKG 1686
Cdd:cd05905    91 ALTVEACLKGLPKKLLKSKtaaeiakkkgWPKIL----DFVKIPKSKRSKlkkwgphPPTRDGDTAYIEYSFSSDGSLSG 166

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 641744967 1687 VMNSHRALCNRLVWMQNTYRLTPDDRVLQKTPFSFDVSVWefFWPLL 1733
Cdd:cd05905   167 VAVSHSSLLAHCRALKEACELYESRPLVTVLDFKSGLGLW--HGCLL 211
PTZ00216 PTZ00216
acyl-CoA synthetase; Provisional
 1531-1694 1.88e-06

acyl-CoA synthetase; Provisional


Pssm-ID: 240316 [Multi-domain]  Cd Length: 700  Bit Score: 53.83  E-value: 1.88e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1531 HQLVEDQ--AARTPDTTaVLFEDQHLTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAG--AAYV 1606
Cdd:PTZ00216   98 KEVVKDAdgKERTMEVT-HFNETRYITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSmvAATV 176

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1607 PLDPGYPAerLAYMLDDASPVALLTQA-NQRALLT----GDVPR---ILLDT--ADF------------------SHLSE 1658
Cdd:PTZ00216  177 YANLGEDA--LAYALRETECKAIVCNGkNVPNLLRlmksGGMPNttiIYLDSlpASVdtegcrlvawtdvvakghSAGSH 254

                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 641744967 1659 DNPHVPGlDAHHLAYVIYTSGSTGKPKGVMNSHRAL 1694
Cdd:PTZ00216  255 HPLNIPE-NNDDLALIMYTSGTTGDPKGVMHTHGSL 289
PLN02736 PLN02736
long-chain acyl-CoA synthetase
 616-919 1.91e-06

long-chain acyl-CoA synthetase


Pssm-ID: 178337 [Multi-domain]  Cd Length: 651  Bit Score: 53.56  E-value: 1.91e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  616 LAYVIYTSGSTGRPKGVMVAHRNVINLATGlHTLLALDHPSRIAL------------NASIVFDASVKnwIQLLSGHTLV 683
Cdd:PLN02736  223 VATICYTSGTTGTPKGVVLTHGNLIANVAG-SSLSTKFYPSDVHIsylplahiyervNQIVMLHYGVA--VGFYQGDNLK 299

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  684 LVPD--ALR----ADAHQLW-------------------RYFA------RHAvdLFDCTPVQLQW------LLDAGLGSD 726
Cdd:PLN02736  300 LMDDlaALRptifCSVPRLYnriydgitnavkesgglkeRLFNaaynakKQA--LENGKNPSPMWdrlvfnKIKAKLGGR 377

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  727 PAYqpaqVLIGGEAISPAVWSRLQSLSDTRFINVYGPTEctvdaTACVVDRTQP----LPTIGKPLANTRLYILD----- 797
Cdd:PLN02736  378 VRF----MSSGASPLSPDVMEFLRICFGGRVLEGYGMTE-----TSCVISGMDEgdnlSGHVGSPNPACEVKLVDvpemn 448

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  798 --AQDQPVPigvTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGR--NDFQIkV 873
Cdd:PLN02736  449 ytSEDQPYP---RGEICVRGPIIFKGYYKDEVQTREVIDEDGW--------LHTGDIGLWLPGGRLKIIDRkkNIFKL-A 516

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*....
gi 641744967  874 RGFRIEAGEIESRLLRCPGVQDAVVIaredspGDT---RLVAYLCARPD 919
Cdd:PLN02736  517 QGEYIAPEKIENVYAKCKFVAQCFVY------GDSlnsSLVAVVVVDPE 559
PRK09294 PRK09294
phthiocerol/phthiodiolone dimycocerosyl transferase;
1096-1326 2.85e-06

phthiocerol/phthiodiolone dimycocerosyl transferase;


Pssm-ID: 181765 [Multi-domain]  Cd Length: 416  Bit Score: 52.79  E-value: 2.85e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1096 ALHLTGRLDRPALTTALNGLVARHESLRTRFtsidgqpaqQIDPDTLGFSLSSHDLRKLDEAARTTRVAELAEqearARF 1175
Cdd:PRK09294   27 TAHLRGVLDIDALSDAFDALLRAHPVLAAHL---------EQDSDGGWELVADDLLHPGIVVVDGDAARPLPE----LQL 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1176 DLTQGpLIRGQLLQLDDNTHVLLLTqHHIISDGWSIGILARELAALYQAALEGSEAnlPPLPVQYADYAvwqrqwlqGET 1255
Cdd:PRK09294   94 DQGVS-LLALDVVPDDGGARVTLYI-HHSIADAHHSASLLDELWSRYTDVVTTGDP--GPIRPQPAPQS--------LEA 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1256 LNDLRDYWRDQLQGAPALL------EIPTDRPRPSVQRYAGDQVPFH----LDAGQLRRLHALNRQQGTTL-----FMTL 1320
Cdd:PRK09294  162 VLAQRGIRRQALSGAERFMpamyayELPPTPTAAVLAKPGLPQAVPVtrcrLSKAQTSSLAAFGRRHRLTVnalvsAAIL 241


                  ....*.
gi 641744967 1321 LAAWSV 1326
Cdd:PRK09294  242 LAEWQL 247
X-Domain_NRPS cd19546
X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to ...
 2165-2535 3.00e-06

X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to the non-ribosomal peptide synthetase (NRPS); The X-domain is a catalytically inactive member of the Condensation (C) domain family of non-ribosomal peptide synthetase (NRPS). It has been shown to recruit oxygenases to the NRPS to perform side-chain crosslinking in the production of glycopeptide antibiotics. C-domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as this X-domain, the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, and dual E/C (epimerization and condensation) domains. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity; members of this X-domain subfamily lack the second H of this motif.


Pssm-ID: 380468 [Multi-domain]  Cd Length: 440  Bit Score: 52.87  E-value: 3.00e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2165 APLQEGILFHHLL-QEQGDTYLLRSMVAFTHRERLD--AFLSALQQVIDRHDILRT--AVCWQDLSQpvQVVWRQAILPI 2239
Cdd:cd19546     5 VPATAGQLRTWLLaRLDEETRGRHLSVALRLRGRLDrdALEAALGDVAARHEILRTtfPGDGGDVHQ--RILDADAARPE 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2240 NHFEPTSPEDVLAQLQAHTEprtRRIDLSQAPLFRADIAhdPLQN-EWLLALSFHHLISDHMTLALIVGEIRLLLQHQAD 2318
Cdd:cd19546    83 LPVVPATEEELPALLADRAA---HLFDLTRETPWRCTLF--ALSDtEHVLLLVVHRIAADDESLDVLVRDLAAAYGARRE 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2319 A-----LPTPLPYRNFIAQTLSVPNSAHE---------AYFRDKLADVDEPTA-PFGLLNVQGSGGDIHEARLVLDATLA 2383
Cdd:cd19546   158 GraperAPLPLQFADYALWERELLAGEDDrdsligdqiAYWRDALAGAPDELElPTDRPRPVLPSRRAGAVPLRLDAEVH 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2384 SAIRQQARHLGVSPGVLFHVAWAQVLAQTSGRDDVVFGSVlLGRLAGAEGADRIMGMFINTLPLRISL-ADRGAAEVVER 2462
Cdd:cd19546   238 ARLMEAAESAGATMFTVVQAALAMLLTRLGAGTDVTVGTV-LPRDDEEGDLEGMVGPFARPLALRTDLsGDPTFRELLGR 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2463 TSHDLMTLLEHEQAPLALAQRCSGVAPPM---PLFSTLLNYRHTQASSTDNT----LSDIRVLTSEERTNYPLTLAVDDR 2535
Cdd:cd19546   317 VREAVREARRHQDVPFERLAELLALPPSAdrhPVFQVALDVRDDDNDPWDAPelpgLRTSPVPLGTEAMELDLSLALTER 396
PKS_PP smart00823
Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the ...
 3111-3185 5.12e-06

Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the prosthetic group of acyl carrier proteins (ACP) in some multienzyme complexes where it serves as a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups.


Pssm-ID: 214834 [Multi-domain]  Cd Length: 86  Bit Score: 46.86  E-value: 5.12e-06
                            10        20        30        40        50        60        70
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967   3111 EAPQGDLEHALAQIWQTLLGV---ERVGRHDHFFELGGHSLLAVQLNARIRAEFLTDIPIVAIFQHPQLSALAEVILA 3185
Cdd:smart00823    7 AERRRLLLDLVREQVAAVLGHaaaEAIDPDRPFRDLGLDSLMAVELRNRLEAATGLRLPATLVFDHPTPAALAEHLAA 84
PTZ00342 PTZ00342
acyl-CoA synthetase; Provisional
 604-888 5.25e-06

acyl-CoA synthetase; Provisional


Pssm-ID: 240370 [Multi-domain]  Cd Length: 746  Bit Score: 52.41  E-value: 5.25e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  604 TNPVVQGLHAAHLAYVIYTSGSTGRPKGVMVAHRNVINLATGL--HTLLALDHPS-------------RIALNASIVFDA 668
Cdd:PTZ00342  294 TNYKIQNEDPDFITSIVYTSGTSGKPKGVMLSNKNLYNTVVPLckHSIFKKYNPKthlsylpishiyeRVIAYLSFMLGG 373

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  669 SVKNW---IQLLS-------GHTLVLVPDALR----------ADAHQLWRYFARHAVDL---FDCTPvqLQWLLDAGLG- 724
Cdd:PTZ00342  374 TINIWskdINYFSkdiynskGNILAGVPKVFNriytnimteiNNLPPLKRFLVKKILSLrksNNNGG--FSKFLEGITHi 451

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  725 ----SDPAYQPAQVLI-GGEAISPAVWSRLQSLSDTRFINVYGPTECTvdaTACVVDRTQPLPT--IGKPLANTRLY--- 794
Cdd:PTZ00342  452 sskiKDKVNPNLEVILnGGGKLSPKIAEELSVLLNVNYYQGYGLTETT---GPIFVQHADDNNTesIGGPISPNTKYkvr 528

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  795 ---ILDAQDQPvPigvTGELHIGGAGVARGYLHRPDLTAERFIPDPFsadpaariYKTGDLARWLPDGNIDYLGRNDFQI 871
Cdd:PTZ00342  529 tweTYKATDTL-P---KGELLIKSDSIFSGYFLEKEQTKNAFTEDGY--------FKTGDIVQINKNGSLTFLDRSKGLV 596

                         330
                  ....*....|....*...
gi 641744967  872 KvrgfrIEAGE-IESRLL 888
Cdd:PTZ00342  597 K-----LSQGEyIETDML 609
E_NRPS cd19534
Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 66-257 6.49e-06

Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Epimerization (E) domains of nonribosomal peptide synthetases (NRPS) flip the chirality of the end amino acid of a peptide being manufactured by the NRPS. E-domains are homologous to the Condensation (C) domains. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Specialized tailoring NRPS domains such as E-domains greatly increase the range of possible peptide products created by the NRPS machinery. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the E-domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380457 [Multi-domain]  Cd Length: 428  Bit Score: 51.48  E-value: 6.49e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   66 DAFLDVLQQVIARHDILRtAICWQGLHQPVQV----VWRQAPLTVNTLtttSSDTVPAQLRAATDPSNHRLNLSNAPLLS 141
Cdd:cd19534    37 DALRQALRALVEHHDALR-MRFRREDGGWQQRirgdVEELFRLEVVDL---SSLAQAAAIEALAAEAQSSLDLEEGPLLA 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  142 A-----TTAHDpvcgewLLSLSIHHLISDHITQALIIDEIRLLLEDRPEALPKPLP----YRNFI---AQILSVPLSEHE 209
Cdd:cd19534   113 AalfdgTDGGD------RLLLVIHHLVVDGVSWRILLEDLEAAYEQALAGEPIPLPsktsFQTWAellAEYAQSPALLEE 186

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 641744967  210 QYFRNRLADIDTPTAPFDLVDVQGngeDITEARLSLDSSLADALRRQA 257
Cdd:cd19534   187 LAYWRELPAADYWGLPKDPEQTYG---DARTVSFTLDEEETEALLQEA 231
X-Domain_NRPS cd19546
X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to ...
 32-318 6.58e-06

X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to the non-ribosomal peptide synthetase (NRPS); The X-domain is a catalytically inactive member of the Condensation (C) domain family of non-ribosomal peptide synthetase (NRPS). It has been shown to recruit oxygenases to the NRPS to perform side-chain crosslinking in the production of glycopeptide antibiotics. C-domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as this X-domain, the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, and dual E/C (epimerization and condensation) domains. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity; members of this X-domain subfamily lack the second H of this motif.


Pssm-ID: 380468 [Multi-domain]  Cd Length: 440  Bit Score: 51.71  E-value: 6.58e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   32 APLQEGILFHYQL-QEKGDTYLLNSLLAFDSQTRLD--AFLDVLQQVIARHDILRTAICWQG--LHQPVQVVWRQAPLTv 106
Cdd:cd19546     5 VPATAGQLRTWLLaRLDEETRGRHLSVALRLRGRLDrdALEAALGDVAARHEILRTTFPGDGgdVHQRILDADAARPEL- 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  107 nTLTTTSSDTVPAQLRAATDpsnHRLNLS-----NAPLLSATTAhdpvcgEWLLSLSIHHLISDHITQALIIDEIRLLLE 181
Cdd:cd19546    84 -PVVPATEEELPALLADRAA---HLFDLTretpwRCTLFALSDT------EHVLLLVVHRIAADDESLDVLVRDLAAAYG 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  182 DR-----PEALPKPLPYRNFI---------AQILSVPLSEHEQYFRNRLADI-DTPTAPFDLVDVQGNGEDITEARLSLD 246
Cdd:cd19546   154 ARregraPERAPLPLQFADYAlwerellagEDDRDSLIGDQIAYWRDALAGApDELELPTDRPRPVLPSRRAGAVPLRLD 233

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967  247 SSLADALRRQARHLGISSSVLFHVAWAQVLALTSGRDDVVFGSVLSgRLQGNLGADRVMGMFINTLPLRVSL 318
Cdd:cd19546   234 AEVHARLMEAAESAGATMFTVVQAALAMLLTRLGAGTDVTVGTVLP-RDDEEGDLEGMVGPFARPLALRTDL 304
PaaK cd05913
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ...
 1677-1951 8.07e-06

Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.


Pssm-ID: 341239 [Multi-domain]  Cd Length: 425  Bit Score: 51.09  E-value: 8.07e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1677 TSGSTGKPKGVMNSHRALCN------RLVWMQNtyrLTPDDRVLQKTPFSFdvsvWEFFWPLLYGAR----LVMARPDGH 1746
Cdd:cd05913    86 SSGTTGKPTVVGYTKNDLDVwaelvaRCLDAAG---VTPGDRVQNAYGYGL----FTGGLGFHYGAErlgaLVIPAGGGN 158

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1747 KDAayLAQLIERTGITTLHFVPSMLQQFVQWAD---ADCACDSLRRVICSGEALPAELQQRFFARFNAQLHNLYGPTE-- 1821
Cdd:cd05913   159 TER--QLQLIKDFGPTVLCCTPSYALYLAEEAEeegIDPRELSLKVGIFGAEPWTEEMRKRIERRLGIKAYDIYGLTEii 236

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1822 ---AAID------VTFWacqpDDHrsFVPIgrpiantqlYILDTLGQPVPLGVAGELHIggvgvargylnrPDLTAERFi 1892
Cdd:cd05913   237 gpgVAFEceekdgLHIW----EDH--FIPE---------IIDPETGEPVPPGEVGELVF------------TTLTKEAM- 288

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 1893 pdPFINqpgarlYKTGDLARWLPDGSLE---------YLGRNDFQVKLRGFRIELGEIEARLMQCPGV 1951
Cdd:cd05913   289 --PLIR------YRTRDITRLLPGPCPCgrthrridrITGRSDDMLIIRGVNVFPSQIEDVLLKIPGL 348
PRK06814 PRK06814
acyl-[ACP]--phospholipid O-acyltransferase;
2738-3093 8.10e-06

acyl-[ACP]--phospholipid O-acyltransferase;


Pssm-ID: 235865 [Multi-domain]  Cd Length: 1140  Bit Score: 51.89  E-value: 8.10e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2738 PDTPLDARKqgltPRHLAYVIYTSGSTGKPKGVMVEHANMvnfLCSMRKepgiAQEDVLLGVTSLSFDIsiLEIF----- 2812
Cdd:PRK06814  783 PLVYFCNRD----PDDPAVILFTSGSEGTPKGVVLSHRNL---LANRAQ----VAARIDFSPEDKVFNA--LPVFhsfgl 849

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2813 -----LPLLNGARLilatqaqaadaqqlamlierhavsFMQATPSTWRMLVEL-----------RDFAL--------PPG 2868
Cdd:PRK06814  850 tgglvLPLLSGVKV------------------------FLYPSPLHYRIIPELiydtnatilfgTDTFLngyaryahPYD 905

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2869 FKAL----CGGEALPENLATALLQKV-TTLWNLYGPTETtiwSTLNGLTTPTpyigHPIANTQiyildaqGRVVPL---- 2939
Cdd:PRK06814  906 FRSLryvfAGAEKVKEETRQTWMEKFgIRILEGYGVTET---APVIALNTPM----HNKAGTV-------GRLLPGieyr 971

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2940 -----GV--AGEIHIAGAGVVRGYLgRPDltAERFITDPFSGapearMYKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRI 3012
Cdd:PRK06814  972 lepvpGIdeGGRLFVRGPNVMLGYL-RAE--NPGVLEPPADG-----WYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMI 1043

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 3013 ELGEIETRLARCHGVHDAVVIAREDSPGDKRLVayLLAQPDTVLEPADLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKL 3092
Cdd:PRK06814 1044 SLAAVEELAAELWPDALHAAVSIPDARKGERII--LLTTASDATRAAFLAHAKAAGASELMVPAEIITIDEIPLLGTGKI 1121


                  .
gi 641744967 3093 D 3093
Cdd:PRK06814 1122 D 1122
PLN02430 PLN02430
long-chain-fatty-acid-CoA ligase
 1652-1985 8.65e-06

long-chain-fatty-acid-CoA ligase


Pssm-ID: 178049 [Multi-domain]  Cd Length: 660  Bit Score: 51.74  E-value: 8.65e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1652 DFSHLSEDNPHVPGL-DAHHLAYVIYTSGSTGKPKGVMNSHRALCNRLVWM-----QNTYRLTPDDRVLQKTPFS--FDV 1723
Cdd:PLN02430  202 DFLHMGKENPSETNPpKPLDICTIMYTSGTSGDPKGVVLTHEAVATFVRGVdlfmeQFEDKMTHDDVYLSFLPLAhiLDR 281

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1724 SVWEFFWPllYGARLVMArpdgHKDAAYLAQLIERTGITTLHFVPSM-------LQQFVQ-------------------W 1777
Cdd:PLN02430  282 MIEEYFFR--KGASVGYY----HGDLNALRDDLMELKPTLLAGVPRVferihegIQKALQelnprrrlifnalykyklaW 355

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1778 ADADCACDS--------------------LRRVICSGEALPAELQQrfFARFN--AQLHNLYGPTEAAIDVTFwaCQPDD 1835
Cdd:PLN02430  356 MNRGYSHKKaspmadflafrkvkaklggrLRLLISGGAPLSTEIEE--FLRVTscAFVVQGYGLTETLGPTTL--GFPDE 431

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1836 HRSFVPIGRPIANTQLYI-------LDTLGQPVplgvAGELHIGGVGVARGYLNRPDLTAErFIPDPFinqpgarlYKTG 1908
Cdd:PLN02430  432 MCMLGTVGAPAVYNELRLeevpemgYDPLGEPP----RGEICVRGKCLFSGYYKNPELTEE-VMKDGW--------FHTG 498

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1909 DLARWLPDGSLEYLGRNDFQVKL-RGFRIELGEIEARLMQCPGVQEAVVVaredspGDT---RLVAYLCPQPGVTPDPAD 1984
Cdd:PLN02430  499 DIGEILPNGVLKIIDRKKNLIKLsQGEYVALEYLENVYGQNPIVEDIWVY------GDSfksMLVAVVVPNEENTNKWAK 572


                  .
gi 641744967 1985 L 1985
Cdd:PLN02430  573 D 573
PksD COG3321
Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites ...
 672-1204 1.02e-05

Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442550 [Multi-domain]  Cd Length: 1386  Bit Score: 51.80  E-value: 1.02e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  672 NWIQLLSGHTLVLVPDALRADAHQLWryfARHAVDLFDCTPVQLQWLLDAGLGSDPAYQPAQVLIGGEAISPAVWSRLQS 751
Cdd:COG3321   848 DWSALYPGRGRRRVPLPTYPFQREDA---AAALLAAALAAALAAAAALGALLLAALAAALAAALLALAAAAAAALALAAA 924

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  752 LSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANTRLYILDAQDQPVPIGVTGELHIGGAGVARGYLHRPDLTAE 831
Cdd:COG3321   925 ALAALLALVALAAAAAALLALAAAAAAAAAALAAAEAGALLLLAAAAAAAAAAAAAAAAAAAAAAAAAAAALAAAAALAL 1004

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  832 RFIPDPFSADPAARIYKTGDLARWLPDGNIDYLGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDAVVIAREDSPGDTRLV 911
Cdd:COG3321  1005 LAAAALLLAAAAAAAALLALAALLAAAAAALAAAAAAAAAAAALAALAAAAAAAAALALALAALLLLAALAELALAAAAL 1084

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  912 AYLCARPDAELHPAALRQQLAASLADYMIPSAFVTLDALPLTPNGKLDRKALPAPDQTAFATRDYEAPQGGIETALAALW 991
Cdd:COG3321  1085 ALAAALAAAALALALAALAAALLLLALLAALALAAAAAALLALAALLAAAAAAAALAAAAAAAAALALAAAAAALAAALA 1164

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  992 QELLGLDRVGRHDQFFALGGHSLLAVQLLNRMNKAGMDVALATLFAHPTLCDLAAAVTATAHDAPFTLPVADRTQPLPLS 1071
Cdd:COG3321  1165 AALLAAAALLLALALALAAALAAALAGLAALLLAALLAALLAALLALALAALAAAAAALLAAAAAAAALALLALAAAAAA 1244

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1072 FSQQRLWFLAQLDPAASQAYHLPAALHLTGRLDRPALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLGFSLSSHDL 1151
Cdd:COG3321  1245 VAALAAAAAALLAALAALALLAAAAGLAALAAAAAAAAAALALAAAAAAAAAALAALLAAAAAAAAAAAAAAAAAALAAA 1324

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|...
gi 641744967 1152 RKLDEAARTTRVAELAEQEARARFDLTQGPLIRGQLLQLDDNTHVLLLTQHHI 1204
Cdd:COG3321  1325 LLAAALAALAAAVAAALALAAAAAAAAAAAAAAAAAAALAAAAGAAAAAAALA 1377
LC_FACS_bac1 cd17641
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ...
 2633-3055 1.37e-05

bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341296 [Multi-domain]  Cd Length: 569  Bit Score: 50.88  E-value: 1.37e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2633 SYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQS- 2711
Cdd:cd17641    13 TWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIAEDe 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2712 -------AHLGIMNGSLPVILLDD------------------GETRPFDNEPDTPLDARKQGLTPRHLAYVIYTSGSTGK 2766
Cdd:cd17641    93 eqvdkllEIADRIPSVRYVIYCDPrgmrkyddprlisfedvvALGRALDRRDPGLYEREVAAGKGEDVAVLCTTSGTTGK 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2767 PKGVMVEHANMvnflcsmrkepgIAQEDVLLGVTSLSFDISILEiFLPLLNGARLILATQAQaadaqqlamLIERHAVSF 2846
Cdd:cd17641   173 PKLAMLSHGNF------------LGHCAAYLAADPLGPGDEYVS-VLPLPWIGEQMYSVGQA---------LVCGFIVNF 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2847 ------------------MQATPSTW---------RM----------------------------------LVELRDFAL 2865
Cdd:cd17641   231 peepetmmedlreigptfVLLPPRVWegiaadvraRMmdatpfkrfmfelgmklglraldrgkrgrpvslwLRLASWLAD 310

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2866 PPGFKAL-------------CGGEALPENLATALLQKVTTLWNLYGPTETTIWSTLN-GLTTPTPYIGHPIANTQIYILD 2931
Cdd:cd17641   311 ALLFRPLrdrlgfsrlrsaaTGGAALGPDTFRFFHAIGVPLKQLYGQTELAGAYTVHrDGDVDPDTVGVPFPGTEVRIDE 390

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2932 AqgrvvplgvaGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGR-NDFQVKVRGF 3010
Cdd:cd17641   391 V----------GEILVRSPGVFVGYYKNPEATAEDFDEDGW--------LHTGDAGYFKENGHLVVIDRaKDVGTTSDGT 452

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*
gi 641744967 3011 RIELGEIETRLARCHGVHDAVVIARedspGDKRLVAYLLAQPDTV 3055
Cdd:cd17641   453 RFSPQFIENKLKFSPYIAEAVVLGA----GRPYLTAFICIDYAIV 493
PaaK COG1541
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ...
 710-960 1.51e-05

Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];


Pssm-ID: 441150 [Multi-domain]  Cd Length: 423  Bit Score: 50.53  E-value: 1.51e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  710 CTPVQLQWLLDAG--LGSDPA-YQPAQVLIGGEAISPAVWSRLQSLSDTRFINVYGPTECTVD-ATACvvdRTQP----- 780
Cdd:COG1541   181 GTPSYLLYLAEVAeeEGIDPRdLSLKKGIFGGEPWSEEMRKEIEERWGIKAYDIYGLTEVGPGvAYEC---EAQDglhiw 257

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  781 ----LPTIGKPlaNTrlyildaqDQPVPIGVTGELHIGGagvargylhrpdLTAERFipdpfsadPAARiYKTGDLARWL 856
Cdd:COG1541   258 edhfLVEIIDP--ET--------GEPVPEGEEGELVVTT------------LTKEAM--------PLIR-YRTGDLTRLL 306

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  857 PDGN--------IDY-LGRNDFQIKVRGFRIEAGEIESRLLRCPGVQDA--VVIAREDspGDTRLVAYLCARPDAELhpA 925
Cdd:COG1541   307 PEPCpcgrthprIGRiLGRADDMLIIRGVNVFPSQIEEVLLRIPEVGPEyqIVVDREG--GLDELTVRVELAPGASL--E 382

                         250       260       270
                  ....*....|....*....|....*....|....*...
gi 641744967  926 ALRQQLAASLADYMIPSA---FVTLDALPLTPnGKLDR 960
Cdd:COG1541   383 ALAEAIAAALKAVLGLRAeveLVEPGSLPRSE-GKAKR 419
PP-binding pfam00550
Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached ...
 2044-2101 1.76e-05

Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. This family includes members not included in Prosite. The inclusion of these members is supported by sequence analysis and functional evidence. The related domain of Swiss:P19828 has the attachment serine replaced by an alanine.


Pssm-ID: 425746 [Multi-domain]  Cd Length: 62  Bit Score: 44.86  E-value: 1.76e-05
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 641744967  2044 ALAAVWQDLLGLT--RVGRHDHFFALGGHSLLIVSLIERLRRA-GLALDVRGVFSTPVLSD 2101
Cdd:pfam00550    2 RLRELLAEVLGVPaeEIDPDTDLFDLGLDSLLAVELIARLEEEfGVEIPPSDLFEHPTLAE 62
PRK05691 PRK05691
peptide synthase; Validated
 54-331 2.21e-05

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 50.55  E-value: 2.21e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967   54 NSLLAFDSQTRLDAFL--DVLQQVIARHDILRTAIC-----WQGLHQPV---QVVWRqapLTVNTLTTTSSDTVPAQlra 123
Cdd:PRK05691 2813 NQALLLEPRQALDPALleQALQALVEHHDALRLRFSqadgrWQAEYRAVtaqELLWQ---VTVADFAECAALFADAQ--- 2886

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  124 atdpsnHRLNLSNAPLLSATTAHDPVCGEWLLsLSIHHLISDHITQALIIDEI----RLLLEDRPEALP-KPLPYRNFIA 198
Cdd:PRK05691 2887 ------RSLDLQQGPLLRALLVDGPQGQQRLL-LAIHHLVVDGVSWRVLLEDLqalyRQLSAGAEPALPaKTSAFRDWAA 2959

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  199 QIL----SVPLSEHEQYFRNRLADIDTpTAPFDLVDVQGNGEDITEARLSLDSSLADALRRQ---ARHLGISSSVLfhVA 271
Cdd:PRK05691 2960 RLQayagSESLREELGWWQAQLGGPRA-ELPCDRPQGGNLNRHAQTVSVRLDAERTRQLLQQapaAYRTQVNDLLL--TA 3036

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 641744967  272 WAQVLALTSGRDDVVFGSVLSGR--LQGNLGADRVMGMFINTLPLRvsLRERSVHDVVQATS 331
Cdd:PRK05691 3037 LARVLCRWSGQPSVLVQLEGHGReaLFDDIDLTRSVGWFTSAYPLR--LTPAPGDDAARGES 3096
Dip2 cd05905
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of ...
 2631-2823 5.33e-05

Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of the adenylate forming enzyme family, including insect luciferase, acetyl CoA ligases and the adenylation domain of nonribosomal peptide synthetases (NRPS). However, its function may have diverged from other members of the superfamily. In mouse embryo, Dip2 homolog A plays an important role in the development of both vertebrate and invertebrate nervous systems. Dip2A appears to regulate cell growth and the arrangement of cells in organs. Biochemically, Dip2A functions as a receptor of FSTL1, an extracellular glycoprotein, and may play a role as a cardiovascular protective agent.


Pssm-ID: 341231 [Multi-domain]  Cd Length: 571  Bit Score: 48.88  E-value: 5.33e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2631 SLSYDELNRRANRLAHHLI-SFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYML-----DDAKP 2704
Cdd:cd05905    14 TLTWGKLLSRAEKIAAVLQkKVGLKPGDRVALMYPDPLDFVAAFYGCLYAGVVPIPIEPPDISQQLGFLLgtckvRVALT 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2705 VALISqSAHLGIMNGSLPVILLDDGETRP----FDNEP-DTPLDARKQGLTPR----HLAYVIYTSGSTGKPKGVMVEHA 2775
Cdd:cd05905    94 VEACL-KGLPKKLLKSKTAAEIAKKKGWPkildFVKIPkSKRSKLKKWGPHPPtrdgDTAYIEYSFSSDGSLSGVAVSHS 172

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 641744967 2776 NMVNFlCSMRKEPGIAQE-DVLLGVTSLSFDIS-ILEIFLPLLNGARLIL 2823
Cdd:cd05905   173 SLLAH-CRALKEACELYEsRPLVTVLDFKSGLGlWHGCLLSVYSGHHTIL 221
PKS_PP smart00823
Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the ...
 972-1050 7.47e-05

Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the prosthetic group of acyl carrier proteins (ACP) in some multienzyme complexes where it serves as a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups.


Pssm-ID: 214834 [Multi-domain]  Cd Length: 86  Bit Score: 43.78  E-value: 7.47e-05
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967    972 ATRDYEAPQGGIETALAALWQELLGL---DRVGRHDQFFALGGHSLLAVQLLNRMNKA-GMDVALATLFAHPTLCDLAAA 1047
Cdd:smart00823    2 AALPPAERRRLLLDLVREQVAAVLGHaaaEAIDPDRPFRDLGLDSLMAVELRNRLEAAtGLRLPATLVFDHPTPAALAEH 81


                    ...
gi 641744967   1048 VTA 1050
Cdd:smart00823   82 LAA 84
PLN02861 PLN02861
long-chain-fatty-acid-CoA ligase
 1554-2005 1.30e-04

long-chain-fatty-acid-CoA ligase


Pssm-ID: 178452 [Multi-domain]  Cd Length: 660  Bit Score: 47.91  E-value: 1.30e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1554 LTYDALNRRANQLAHHLIDLGVKPDDRIAICVERSLDMVIGLLAILKAGAAYVPLDPGYPAERLAYMLDDAS-PVALLTQ 1632
Cdd:PLN02861   78 LTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVEFIINHAEvSIAFVQE 157

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1633 ANQRALLT---------------GDVPRILLDTAD-----------FSHLSEDNPHVPGLDAHHLAYVIYTSGSTGKPKG 1686
Cdd:PLN02861  158 SKISSILSclpkcssnlktivsfGDVSSEQKEEAEelgvscfsweeFSLMGSLDCELPPKQKTDICTIMYTSGTTGEPKG 237

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1687 VMNSHRALCNRLVWMQNTYRLTpdDRVLQKTPFSFDvsvwefFWPLLYGARLVMARPDGHK---------DAAYLAQLIE 1757
Cdd:PLN02861  238 VILTNRAIIAEVLSTDHLLKVT--DRVATEEDSYFS------YLPLAHVYDQVIETYCISKgasigfwqgDIRYLMEDVQ 309

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1758 RTGITTLHFVPSM---------------------------------LQQFVQWADADCACDSLR------------RVIC 1792
Cdd:PLN02861  310 ALKPTIFCGVPRVydriytgimqkissggmlrkklfdfaynyklgnLRKGLKQEEASPRLDRLVfdkikeglggrvRLLL 389

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1793 SGEA-LPAELQQrfFARFN--AQLHNLYGPTEaaidvtfwACQPddhrSFVPIGRPIAntqlyILDTLGQPVPLGVA--- 1866
Cdd:PLN02861  390 SGAApLPRHVEE--FLRVTscSVLSQGYGLTE--------SCGG----CFTSIANVFS-----MVGTVGVPMTTIEArle 450

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1867 ---------------GELHIGGVGVARGYLNRPDLTAERFIPDPFinqpgarlyKTGDLARWLPDGSLEYLGRNDFQVKL 1931
Cdd:PLN02861  451 svpemgydalsdvprGEICLRGNTLFSGYHKRQDLTEEVLIDGWF---------HTGDIGEWQPNGAMKIIDRKKNIFKL 521

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 641744967 1932 -RGFRIELGEIEARLMQCPGVQEAVVVAredSPGDTRLVAYLCPQpgvtpdpadlRQQLGQHLAEYMVPGAFVTL 2005
Cdd:PLN02861  522 sQGEYVAVENLENTYSRCPLIASIWVYG---NSFESFLVAVVVPD----------RQALEDWAANNNKTGDFKSL 583
PLN02861 PLN02861
long-chain-fatty-acid-CoA ligase
 499-963 2.29e-04

long-chain-fatty-acid-CoA ligase


Pssm-ID: 178452 [Multi-domain]  Cd Length: 660  Bit Score: 46.76  E-value: 2.29e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  499 LTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDPAYPAERLAYILDDAAPVALLTQS 578
Cdd:PLN02861   78 LTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVEFIINHAEVSIAFVQE 157

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  579 AQVAQLNSTLP--TVLLDTPAA----------------AACPDTNPVVQ---------GLHAAHLAYVIYTSGSTGRPKG 631
Cdd:PLN02861  158 SKISSILSCLPkcSSNLKTIVSfgdvsseqkeeaeelgVSCFSWEEFSLmgsldcelpPKQKTDICTIMYTSGTTGEPKG 237

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  632 VMVAHRNVI-NLATGLHTLLALDhpsRIA---------LNASIVFD-----------ASVKNW----------IQLLSGH 680
Cdd:PLN02861  238 VILTNRAIIaEVLSTDHLLKVTD---RVAteedsyfsyLPLAHVYDqvietyciskgASIGFWqgdirylmedVQALKPT 314

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  681 TLVLVP---DALRADAHQLWRYFARHAVDLFDCTPVQLQWLLDAGLGSDPAyQP-----------------AQVLIGGEA 740
Cdd:PLN02861  315 IFCGVPrvyDRIYTGIMQKISSGGMLRKKLFDFAYNYKLGNLRKGLKQEEA-SPrldrlvfdkikeglggrVRLLLSGAA 393

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  741 ISPA-VWSRLQSLSDTRFINVYGPTECTVDATACVVDRTQPLPTIGKPLANtrlyiLDAQDQPVP-IGVT-------GEL 811
Cdd:PLN02861  394 PLPRhVEEFLRVTSCSVLSQGYGLTESCGGCFTSIANVFSMVGTVGVPMTT-----IEARLESVPeMGYDalsdvprGEI 468

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  812 HIGGAGVARGYLHRPDLTAERFIPDPFsadpaariyKTGDLARWLPDGNIDYLGRndfqiKVRGFRIEAGE------IES 885
Cdd:PLN02861  469 CLRGNTLFSGYHKRQDLTEEVLIDGWF---------HTGDIGEWQPNGAMKIIDR-----KKNIFKLSQGEyvavenLEN 534

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967  886 RLLRCPGVQD-------------AVVI----ARED------SPGDtrlVAYLCARPDA------ELHPAALRQQLAAS-- 934
Cdd:PLN02861  535 TYSRCPLIASiwvygnsfesflvAVVVpdrqALEDwaannnKTGD---FKSLCKNLKArkyildELNSTGKKLQLRGFem 611

                         570       580       590
                  ....*....|....*....|....*....|
gi 641744967  935 -LADYMIPSAFvTLDALPLTPNGKLDRKAL 963
Cdd:PLN02861  612 lKAIHLEPNPF-DIERDLITPTFKLKRPQL 640
PksD COG3321
Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites ...
 1026-1563 3.50e-04

Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442550 [Multi-domain]  Cd Length: 1386  Bit Score: 46.79  E-value: 3.50e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1026 AGMDVALATLFAHPTLCDLAAAVTATAHDAPFTLPVADRTQPLPLSFSQQRLWFLAQLDPAASQAYHLPAALHLTGRLDR 1105
Cdd:COG3321   843 AGVPVDWSALYPGRGRRRVPLPTYPFQREDAAAALLAAALAAALAAAAALGALLLAALAAALAAALLALAAAAAAALALA 922

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1106 PALTTALNGLVARHESLRTRFTSIDGQPAQQIDPDTLGFSLSSHDLRKLDEAARTTRVAELAEQEARARFDLTQGPLIRG 1185
Cdd:COG3321   923 AAALAALLALVALAAAAAALLALAAAAAAAAAALAAAEAGALLLLAAAAAAAAAAAAAAAAAAAAAAAAAAAALAAAAAL 1002

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1186 QLLQLDDNTHVLLLTQHHIISDGWSIGILARELAALYQAALEGSEANLPPLPVQYADYAVWQRQWLQGETLNDLRDYWRD 1265
Cdd:COG3321  1003 ALLAAAALLLAAAAAAAALLALAALLAAAAAALAAAAAAAAAAAALAALAAAAAAAAALALALAALLLLAALAELALAAA 1082

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1266 QLQGAPALLEIPTDRPRPSVQRYAGDQVPFHLDAGQLRRLHALNRQQGTTLFMTLLAAWSVVLSRLSGQDDIVIGTPVAN 1345
Cdd:COG3321  1083 ALALAAALAAAALALALAALAAALLLLALLAALALAAAAAALLALAALLAAAAAAAALAAAAAAAAALALAAAAAALAAA 1162

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1346 RPRQELEGMVGFFVNTLALRTEPGRCHAVADLLDQVRERALDAYAHQALPFEQVVEILQPARSLSYSPIFQVMLSLNNTP 1425
Cdd:COG3321  1163 LAAALLAAAALLLALALALAAALAAALAGLAALLLAALLAALLAALLALALAALAAAAAALLAAAAAAAALALLALAAAA 1242

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 1426 AQALTLPDLTLSAVERPQhSTHFDLSLSLIETENGLNGGLVYATDLFDRETILRVVGYVENILMAMADDVTQPVATLPIL 1505
Cdd:COG3321  1243 AAVAALAAAAAALLAALA-ALALLAAAAGLAALAAAAAAAAAALALAAAAAAAAAALAALLAAAAAAAAAAAAAAAAAAL 1321

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 641744967 1506 PDSERRQVMLDFNATEADFPHDALIHQLVEDQAARTPDTTAVLFEDQHLTYDALNRRA 1563
Cdd:COG3321  1322 AAALLAAALAALAAAVAAALALAAAAAAAAAAAAAAAAAAALAAAAGAAAAAAALALA 1379
PRK05620 PRK05620
long-chain fatty-acid--CoA ligase;
2621-3097 5.01e-04

long-chain fatty-acid--CoA ligase;


Pssm-ID: 180167 [Multi-domain]  Cd Length: 576  Bit Score: 45.93  E-value: 5.01e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2621 DAIAVVFGEAS---LSYDELNRRANRLAHHLI-SFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLR 2696
Cdd:PRK05620   25 DTTVTTWGGAEqeqTTFAAIGARAAALAHALHdELGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIV 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2697 YMLDDAKPVALISQSA---HLGIMNGSLP-----VILLDDGETRPFDNEP--------DTPLDARKQ-----GLTPRHLA 2755
Cdd:PRK05620  105 HIINHAEDEVIVADPRlaeQLGEILKECPcvravVFIGPSDADSAAAHMPegikvysyEALLDGRSTvydwpELDETTAA 184

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2756 YVIYTSGSTGKPKGVMVEHANMvnFLCSMRkepgiaqedvLLGVTSLSfdISILEIFL-------------PL---LNGA 2819
Cdd:PRK05620  185 AICYSTGTTGAPKGVVYSHRSL--YLQSLS----------LRTTDSLA--VTHGESFLccvpiyhvlswgvPLaafMSGT 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2820 RLILATQAQAADAQQLamLIERHAVSFMQATPSTWRMLVeLRDFALPPGFKAL----CGGEALPENLATALLQK----VT 2891
Cdd:PRK05620  251 PLVFPGPDLSAPTLAK--IIATAMPRVAHGVPTLWIQLM-VHYLKNPPERMSLqeiyVGGSAVPPILIKAWEERygvdVV 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2892 TLWnlyGPTETTIWSTLNglTTPTPYIGHPIANTQIyildAQGRvVPLGV-----------------AGEIHIAGAGVVR 2954
Cdd:PRK05620  328 HVW---GMTETSPVGTVA--RPPSGVSGEARWAYRV----SQGR-FPASLeyrivndgqvmestdrnEGEIQVRGNWVTA 397

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2955 GYL----GRPDLTAERFITDPFSGAPEARM----YKTGDLGRWLPDGTLEYLGRNDFQVKVRGFRIELGEIETRLARCHG 3026
Cdd:PRK05620  398 SYYhsptEEGGGAASTFRGEDVEDANDRFTadgwLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPE 477

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 641744967 3027 VHDAVVIAREDSPGDKRLVAYLLAQPDTvlEPA-----DLRQRLSEGVAEYMIPSAFVTLDAFPLTPNGKLDRKAL 3097
Cdd:PRK05620  478 VVECAVIGYPDDKWGERPLAVTVLAPGI--EPTretaeRLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDL 551
hsFATP4_like cd05939
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ...
 2632-2796 5.28e-04

Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341262 [Multi-domain]  Cd Length: 474  Bit Score: 45.49  E-value: 5.28e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2632 LSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLGILKAGGAYVPLDPTYPVERLRYMLDDAKPVALISQs 2711
Cdd:cd05939     4 WTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALIFN- 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2712 ahlgimngslpviLLDDGETRPFDNEPDTPLdarkQGLTPRhLAYvIYTSGSTGKPKGVMVEHANMVNFLCSMRKEPGIA 2791
Cdd:cd05939    83 -------------LLDPLLTQSSTEPPSQDD----VNFRDK-LFY-IYTSGTTGLPKAAVIVHSRYYRIAAGAYYAFGMR 143


                  ....*
gi 641744967 2792 QEDVL 2796
Cdd:cd05939   144 PEDVV 148
PRK03584 PRK03584
acetoacetate--CoA ligase;
2619-2774 7.44e-04

acetoacetate--CoA ligase;


Pssm-ID: 235134 [Multi-domain]  Cd Length: 655  Bit Score: 45.17  E-value: 7.44e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2619 TPDAIAVVF-----GEASLSYDELNRRANRLAHHLISFGVRPDERVAICVERGLDMVVGLLG------------------ 2675
Cdd:PRK03584   97 RDDRPAIIFrgedgPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLAtaslgaiwsscspdfgvq 176

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2676 ------------ILKAGGAY----VPLDPTYPVERLRYMLDDAKPVALISQSAHLGIMNGSLPVILLDD----GETRPFD 2735
Cdd:PRK03584  177 gvldrfgqiepkVLIAVDGYryggKAFDRRAKVAELRAALPSLEHVVVVPYLGPAAAAAALPGALLWEDflapAEAAELE 256

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 641744967 2736 NEPdTPLDarkqgltprHLAYVIYTSGSTGKPK-------GVMVEH 2774
Cdd:PRK03584  257 FEP-VPFD---------HPLWILYSSGTTGLPKcivhghgGILLEH 292
PRK07868 PRK07868
acyl-CoA synthetase; Validated
 482-555 1.01e-03

acyl-CoA synthetase; Validated


Pssm-ID: 236121 [Multi-domain]  Cd Length: 994  Bit Score: 45.09  E-value: 1.01e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 641744967  482 QAEQTPEAIAVLFEDQHLTYRELNRRANQLAHHLIALGVQPDDRVALCVERSLEMMVGLLGILKAGAAYVPMDP 555
Cdd:PRK07868  456 QARDAPKGEFLLFDGRVHTYEAVNRRINNVVRGLIAVGVRQGDRVGVLMETRPSALVAIAALSRLGAVAVLMPP 529
PLN02736 PLN02736
long-chain acyl-CoA synthetase
 2751-2778 1.32e-03

long-chain acyl-CoA synthetase


Pssm-ID: 178337 [Multi-domain]  Cd Length: 651  Bit Score: 44.32  E-value: 1.32e-03
                          10        20
                  ....*....|....*....|....*...
gi 641744967 2751 PRHLAYVIYTSGSTGKPKGVMVEHANMV 2778
Cdd:PLN02736  220 PEDVATICYTSGTTGTPKGVVLTHGNLI 247
Anticodon_Ia_Cys cd07963
Anticodon-binding domain of cysteinyl tRNA synthetases; This domain is found in cysteinyl tRNA ...
 486-519 1.51e-03

Anticodon-binding domain of cysteinyl tRNA synthetases; This domain is found in cysteinyl tRNA synthetases (CysRS), which belong to the class Ia aminoacyl tRNA synthetases. It lies C-terminal to the catalytic core domain, and recognizes and specifically binds to the tRNA anticodon. CysRS catalyzes the transfer of cysteine to the 3'-end of its tRNA.


Pssm-ID: 153417 [Multi-domain]  Cd Length: 156  Bit Score: 41.78  E-value: 1.51e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 641744967  486 TPEAIAVLFEdqhLTyRELNR-------RANQLAHHLIALG 519
Cdd:cd07963    48 TPEALAVLFE---LA-REINRlkkedieKAAALAALLKALG 84
PLN02861 PLN02861
long-chain-fatty-acid-CoA ligase
 2943-3024 2.38e-03

long-chain-fatty-acid-CoA ligase


Pssm-ID: 178452 [Multi-domain]  Cd Length: 660  Bit Score: 43.68  E-value: 2.38e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2943 GEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmyKTGDLGRWLPDGTLEYLGRndfqvKVRGFRIELGE------ 3016
Cdd:PLN02861  466 GEICLRGNTLFSGYHKRQDLTEEVLIDGWF---------HTGDIGEWQPNGAMKIIDR-----KKNIFKLSQGEyvaven 531


                  ....*...
gi 641744967 3017 IETRLARC 3024
Cdd:PLN02861  532 LENTYSRC 539
PTZ00342 PTZ00342
acyl-CoA synthetase; Provisional
 2751-3021 2.50e-03

acyl-CoA synthetase; Provisional


Pssm-ID: 240370 [Multi-domain]  Cd Length: 746  Bit Score: 43.55  E-value: 2.50e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2751 PRHLAYVIYTSGSTGKPKGVMVEHANMVNFL-----CSMRK-----------------EPGIAQEDVLLGVT--SLSFDI 2806
Cdd:PTZ00342  303 PDFITSIVYTSGTSGKPKGVMLSNKNLYNTVvplckHSIFKkynpkthlsylpishiyERVIAYLSFMLGGTinIWSKDI 382

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2807 SILEifLPLLNGARLILATQAQAADAQQLAMLIERHAVSFMQatpstwRMLVElRDFALPPGFKA--------------- 2871
Cdd:PTZ00342  383 NYFS--KDIYNSKGNILAGVPKVFNRIYTNIMTEINNLPPLK------RFLVK-KILSLRKSNNNggfskflegithiss 453

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2872 -------------LCGGEALPENLATALlqkvTTLWNL-----YGPTETT--IWSTlNGLTTPTPYIGHPIANTQIY--- 2928
Cdd:PTZ00342  454 kikdkvnpnleviLNGGGKLSPKIAEEL----SVLLNVnyyqgYGLTETTgpIFVQ-HADDNNTESIGGPISPNTKYkvr 528

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2929 ---ILDAQGRvVPlgvAGEIHIAGAGVVRGYLGRPDLTAERFITDPFsgapearmYKTGDLGRWLPDGTLEYLGRNDFQV 3005
Cdd:PTZ00342  529 tweTYKATDT-LP---KGELLIKSDSIFSGYFLEKEQTKNAFTEDGY--------FKTGDIVQINKNGSLTFLDRSKGLV 596

                         330
                  ....*....|....*..
gi 641744967 3006 KvrgfrIELGE-IETRL 3021
Cdd:PTZ00342  597 K-----LSQGEyIETDM 608
PLN02614 PLN02614
long-chain acyl-CoA synthetase
 2918-3016 2.72e-03

long-chain acyl-CoA synthetase


Pssm-ID: 166255 [Multi-domain]  Cd Length: 666  Bit Score: 43.47  E-value: 2.72e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 641744967 2918 IGHPIANTQIYI-------LDAQGRVVplgvAGEIHIAGAGVVRGYLGRPDLTAERFITDpfsgapearMYKTGDLGRWL 2990
Cdd:PLN02614  441 VGPPVPNVDIRLesvpemeYDALASTP----RGEICIRGKTLFSGYYKREDLTKEVLIDG---------WLHTGDVGEWQ 507

                          90       100
                  ....*....|....*....|....*.
gi 641744967 2991 PDGTLEYLGRndfqvKVRGFRIELGE 3016
Cdd:PLN02614  508 PNGSMKIIDR-----KKNIFKLSQGE 528
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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