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Conserved domains on  [gi|400275803|gb|AFP79262|]
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transcription termination factor NusG [Mycoplasmoides gallisepticum WI01_2001.043-13-2P]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NusG_myco TIGR01956
NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the ...
 8-265 1.28e-119

NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the bacterial transcription termination/antitermination factor NusG. These sequences from Mycoplasma are notably diverged (long branches in a Neighbor-joining phylogenetic tree) from the bacterial species. And although NusA and ribosomal protein S10 (NusE) appear to be present, NusB may be absent in Mycoplasmas calling into question whether these species have a functional Nus system including this family as a member.


:

Pssm-ID: 273895 [Multi-domain]  Cd Length: 258  Bit Score: 342.33  E-value: 1.28e-119
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803    8 QWYIATTTNGNEDSVIKTLKAKVRALHFEDQILDCKVIKFRSVEETIFDSNNptHNIPATMRNSTYIKWVTVDNGVYKKY 87
Cdd:TIGR01956   1 QWYIATTINGNEDEVIENIKAKVRALGLENYISDFKILKEREIEEKVFEPKN--GQAPRSMKNTATTKWETLDETKYKKT 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803   88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGKGAKPIPISSS-EELDLLNGESFDQNYRIVITPNAIIEMD 166
Cdd:TIGR01956  79 KISEKNKYNGYIYIKMIMTEDAWFLIRNTENVTGLVGSSGKGAKPIPISADaDKLKMLKGISENTKKRVLVTNTAIVEME 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803  167 RNLFNERGELILDENAAKTIVHKKKSDSADKYGDMSTEKEQVDEAIELKVGHMVDINSGDYSGLSGQISRIIDN-DEYIV 245
Cdd:TIGR01956 159 ENKFDEKCQYILKHKQVKPEAIAQVSESGEIIDEIVEEFQLVDNLSKFRVGNFVKIVDGPFKGIVGKIKKIDQEkKKAIV 238

                         250       260
                  ....*....|....*....|
gi 400275803  246 DVQILGKLVSVKLNKKQLKL 265
Cdd:TIGR01956 239 EVEILGKSVDVDLNFKHLKL 258
 
Name Accession Description Interval E-value
NusG_myco TIGR01956
NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the ...
 8-265 1.28e-119

NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the bacterial transcription termination/antitermination factor NusG. These sequences from Mycoplasma are notably diverged (long branches in a Neighbor-joining phylogenetic tree) from the bacterial species. And although NusA and ribosomal protein S10 (NusE) appear to be present, NusB may be absent in Mycoplasmas calling into question whether these species have a functional Nus system including this family as a member.


Pssm-ID: 273895 [Multi-domain]  Cd Length: 258  Bit Score: 342.33  E-value: 1.28e-119
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803    8 QWYIATTTNGNEDSVIKTLKAKVRALHFEDQILDCKVIKFRSVEETIFDSNNptHNIPATMRNSTYIKWVTVDNGVYKKY 87
Cdd:TIGR01956   1 QWYIATTINGNEDEVIENIKAKVRALGLENYISDFKILKEREIEEKVFEPKN--GQAPRSMKNTATTKWETLDETKYKKT 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803   88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGKGAKPIPISSS-EELDLLNGESFDQNYRIVITPNAIIEMD 166
Cdd:TIGR01956  79 KISEKNKYNGYIYIKMIMTEDAWFLIRNTENVTGLVGSSGKGAKPIPISADaDKLKMLKGISENTKKRVLVTNTAIVEME 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803  167 RNLFNERGELILDENAAKTIVHKKKSDSADKYGDMSTEKEQVDEAIELKVGHMVDINSGDYSGLSGQISRIIDN-DEYIV 245
Cdd:TIGR01956 159 ENKFDEKCQYILKHKQVKPEAIAQVSESGEIIDEIVEEFQLVDNLSKFRVGNFVKIVDGPFKGIVGKIKKIDQEkKKAIV 238

                         250       260
                  ....*....|....*....|
gi 400275803  246 DVQILGKLVSVKLNKKQLKL 265
Cdd:TIGR01956 239 EVEILGKSVDVDLNFKHLKL 258
NGN_Bact_1 cd09891
Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The ...
 8-145 4.29e-30

Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination in bacteria. NusG is essential in Escherichia coli and associates with RNA polymerase elongation and Rho-termination. Homologs of the NusG gene exist in all bacteria. The NusG N-terminal domain (NGN) is similar in all NusG homologs, but its C-terminal domain and the linker that separates these two domains are different. The domain organization of NusG suggests that the common properties of NusG and its homologs are due to their similar NGN domains.


Pssm-ID: 193580 [Multi-domain]  Cd Length: 107  Bit Score: 108.72  E-value: 4.29e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803   8 QWYIATTTNGNEDSVIKTLKAKVRALHFEDQILDCKVIKFRSVEetifdsnnpthnipatMRNstyikwvtvdngvyKKY 87
Cdd:cd09891    1 KWYVVHTYSGYENKVKENLEKRIESEGLEDYIGEVLVPTEEVVE----------------VKN--------------GKK 50

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 400275803  88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSsgkGAKPIPISSSEELDLLN 145
Cdd:cd09891   51 KVKERKLFPGYVLVEMDMNDDTWHLVRNTPGVTGFVGS---GGKPVPLSEEEVERILG 105
NGN smart00738
In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, ...
 8-145 4.76e-24

In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, this domain may confer affinity for Spt4p.Spt4p


Pssm-ID: 197850 [Multi-domain]  Cd Length: 106  Bit Score: 93.21  E-value: 4.76e-24
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803     8 QWYIATTTNGNEDSVIKTLKAKVRALHFEDQILDCKVIKfRSVEEtifdsnnpthnipatMRNStyikwvtvdngvykKY 87
Cdd:smart00738   1 NWYAVRTTSGQEKRVAENLERKAEALGLEDKIVSILVPT-EEVKE---------------IRRG--------------KK 50

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 400275803    88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSsgkGAKPIPISSSEELDLLN 145
Cdd:smart00738  51 KVVERKLFPGYIFVEADLEDEVWTAIRGTPGVRGFVGG---GGKPTPVPDDEIEKILK 105
NusG pfam02357
Transcription termination factor nusG;
8-139 3.90e-13

Transcription termination factor nusG;


Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 63.78  E-value: 3.90e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803    8 QWYIATTTNGNEDSVIKTLKAkvralhfedQILDCKVikfrsveetifdsnnPThnipatmrnstyIKWVTVDNGvykKY 87
Cdd:pfam02357   2 KWYVLQTYSGKEKKVKENLER---------QGIEVFL---------------PT------------EEVVEIRNG---KK 42

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 400275803   88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGkgaKPIPISSSE 139
Cdd:pfam02357  43 KVVERPLFPGYVFVRMDMTDETWHLVRSTPGVTGFVGGSG---KPTPIPDEE 91
NusG COG0250
Transcription termination/antitermination protein NusG [Transcription];
86-264 4.14e-13

Transcription termination/antitermination protein NusG [Transcription];


Pssm-ID: 440020 [Multi-domain]  Cd Length: 171  Bit Score: 65.62  E-value: 4.14e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803  86 KYKITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGkgaKPIPISSSeeldllngesfdqnyrivitpnaiiEM 165
Cdd:COG0250   47 KKKTVERPLFPGYVFVRMDLTDESWYLVRNTPGVTGFVGFGG---KPAPLPDE-------------------------EV 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803 166 DRnlfnergelILdenaaktivhkkksdsadkyGDMSTEKEQVDEAIELKVGHMVDINSGDYSGLSGQISRI-IDNDEYI 244
Cdd:COG0250   99 ER---------IL--------------------ARLEEGEEKPRPKVDFEVGDRVRITDGPFAGFEGTVEEVdPEKGRVK 149

                        170       180
                 ....*....|....*....|
gi 400275803 245 VDVQILGKLVSVKLNKKQLK 264
Cdd:COG0250  150 VLVSIFGRETPVELDFSQVE 169
 
Name Accession Description Interval E-value
NusG_myco TIGR01956
NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the ...
 8-265 1.28e-119

NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the bacterial transcription termination/antitermination factor NusG. These sequences from Mycoplasma are notably diverged (long branches in a Neighbor-joining phylogenetic tree) from the bacterial species. And although NusA and ribosomal protein S10 (NusE) appear to be present, NusB may be absent in Mycoplasmas calling into question whether these species have a functional Nus system including this family as a member.


Pssm-ID: 273895 [Multi-domain]  Cd Length: 258  Bit Score: 342.33  E-value: 1.28e-119
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803    8 QWYIATTTNGNEDSVIKTLKAKVRALHFEDQILDCKVIKFRSVEETIFDSNNptHNIPATMRNSTYIKWVTVDNGVYKKY 87
Cdd:TIGR01956   1 QWYIATTINGNEDEVIENIKAKVRALGLENYISDFKILKEREIEEKVFEPKN--GQAPRSMKNTATTKWETLDETKYKKT 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803   88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGKGAKPIPISSS-EELDLLNGESFDQNYRIVITPNAIIEMD 166
Cdd:TIGR01956  79 KISEKNKYNGYIYIKMIMTEDAWFLIRNTENVTGLVGSSGKGAKPIPISADaDKLKMLKGISENTKKRVLVTNTAIVEME 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803  167 RNLFNERGELILDENAAKTIVHKKKSDSADKYGDMSTEKEQVDEAIELKVGHMVDINSGDYSGLSGQISRIIDN-DEYIV 245
Cdd:TIGR01956 159 ENKFDEKCQYILKHKQVKPEAIAQVSESGEIIDEIVEEFQLVDNLSKFRVGNFVKIVDGPFKGIVGKIKKIDQEkKKAIV 238

                         250       260
                  ....*....|....*....|
gi 400275803  246 DVQILGKLVSVKLNKKQLKL 265
Cdd:TIGR01956 239 EVEILGKSVDVDLNFKHLKL 258
NGN_Bact_1 cd09891
Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The ...
 8-145 4.29e-30

Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination in bacteria. NusG is essential in Escherichia coli and associates with RNA polymerase elongation and Rho-termination. Homologs of the NusG gene exist in all bacteria. The NusG N-terminal domain (NGN) is similar in all NusG homologs, but its C-terminal domain and the linker that separates these two domains are different. The domain organization of NusG suggests that the common properties of NusG and its homologs are due to their similar NGN domains.


Pssm-ID: 193580 [Multi-domain]  Cd Length: 107  Bit Score: 108.72  E-value: 4.29e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803   8 QWYIATTTNGNEDSVIKTLKAKVRALHFEDQILDCKVIKFRSVEetifdsnnpthnipatMRNstyikwvtvdngvyKKY 87
Cdd:cd09891    1 KWYVVHTYSGYENKVKENLEKRIESEGLEDYIGEVLVPTEEVVE----------------VKN--------------GKK 50

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 400275803  88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSsgkGAKPIPISSSEELDLLN 145
Cdd:cd09891   51 KVKERKLFPGYVLVEMDMNDDTWHLVRNTPGVTGFVGS---GGKPVPLSEEEVERILG 105
NGN smart00738
In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, ...
 8-145 4.76e-24

In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, this domain may confer affinity for Spt4p.Spt4p


Pssm-ID: 197850 [Multi-domain]  Cd Length: 106  Bit Score: 93.21  E-value: 4.76e-24
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803     8 QWYIATTTNGNEDSVIKTLKAKVRALHFEDQILDCKVIKfRSVEEtifdsnnpthnipatMRNStyikwvtvdngvykKY 87
Cdd:smart00738   1 NWYAVRTTSGQEKRVAENLERKAEALGLEDKIVSILVPT-EEVKE---------------IRRG--------------KK 50

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 400275803    88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSsgkGAKPIPISSSEELDLLN 145
Cdd:smart00738  51 KVVERKLFPGYIFVEADLEDEVWTAIRGTPGVRGFVGG---GGKPTPVPDDEIEKILK 105
NusG pfam02357
Transcription termination factor nusG;
8-139 3.90e-13

Transcription termination factor nusG;


Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 63.78  E-value: 3.90e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803    8 QWYIATTTNGNEDSVIKTLKAkvralhfedQILDCKVikfrsveetifdsnnPThnipatmrnstyIKWVTVDNGvykKY 87
Cdd:pfam02357   2 KWYVLQTYSGKEKKVKENLER---------QGIEVFL---------------PT------------EEVVEIRNG---KK 42

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 400275803   88 KITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGkgaKPIPISSSE 139
Cdd:pfam02357  43 KVVERPLFPGYVFVRMDMTDETWHLVRSTPGVTGFVGGSG---KPTPIPDEE 91
NusG COG0250
Transcription termination/antitermination protein NusG [Transcription];
86-264 4.14e-13

Transcription termination/antitermination protein NusG [Transcription];


Pssm-ID: 440020 [Multi-domain]  Cd Length: 171  Bit Score: 65.62  E-value: 4.14e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803  86 KYKITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGkgaKPIPISSSeeldllngesfdqnyrivitpnaiiEM 165
Cdd:COG0250   47 KKKTVERPLFPGYVFVRMDLTDESWYLVRNTPGVTGFVGFGG---KPAPLPDE-------------------------EV 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803 166 DRnlfnergelILdenaaktivhkkksdsadkyGDMSTEKEQVDEAIELKVGHMVDINSGDYSGLSGQISRI-IDNDEYI 244
Cdd:COG0250   99 ER---------IL--------------------ARLEEGEEKPRPKVDFEVGDRVRITDGPFAGFEGTVEEVdPEKGRVK 149

                        170       180
                 ....*....|....*....|
gi 400275803 245 VDVQILGKLVSVKLNKKQLK 264
Cdd:COG0250  150 VLVSIFGRETPVELDFSQVE 169
nusG TIGR00922
transcription termination/antitermination factor NusG; NusG proteins are transcription factors ...
 9-264 6.08e-13

transcription termination/antitermination factor NusG; NusG proteins are transcription factors which are aparrently universal in prokaryotes (archaea and eukaryotes have homologs that may have related functions). The essential components of these factors include an N-terminal RNP-like (ribonucleoprotein) domain and a C-terminal KOW motif (pfam00467) believed to be a nucleic acid binding domain. In E. coli, NusA has been shown to interact with RNA polymerase and termination factor Rho. This model covers a wide variety of bacterial species but excludes mycoplasmas which are covered by a separate model (TIGR01956).The function of all of these NusG proteins is likely to be the same at the level of interaction with RNA and other protein factors to affect termination; however different species may utilize NusG towards different processes and in combination with different suites of affector proteins.In E. coli, NusG promotes rho-dependent termination. It is an essential gene. In Streptomyces virginiae and related species, an additional N-terminal sequence is also present and is suggested to play a role in butyrolactone-mediated autoregulation. In Thermotoga maritima, NusG has a long insert, fails to substitute for E. coli NusG (with or without the long insert), is a large 0.7 % of total cellular protein, and has a general, sequence non-specific DNA and RNA binding activity that blocks ethidium staining, yet permits transcription.Archaeal proteins once termed NusG share the KOW domain but are actually a ribosomal protein corresponding to L24p in bacterial and L26e in eukaryotes (TIGR00405). [Transcription, Transcription factors]


Pssm-ID: 273341 [Multi-domain]  Cd Length: 172  Bit Score: 65.40  E-value: 6.08e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803    9 WYIATTTNGNEDSVIKTLKAKVRALHFEDQILDCKVikfrsveetifdsnnPTHNIpatmrnstyikwVTVDNGvykKYK 88
Cdd:TIGR00922   1 WYVVQTYSGYEKKVKQNLEELIELLGMGDYIFEVIV---------------PTEEV------------VEIKKG---KKK 50

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803   89 ITDTNKYPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGkgaKPIPISSSEELDllngesfdqnyrivitpnaiiemdrN 168
Cdd:TIGR00922  51 VVERKIFPGYVLVKMDLTDVSWHLVKNTPGVTGFVGSGG---KPKALSEDEEVK-------------------------N 102

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 400275803  169 LFNERgelildENAAKTIVHKkksdsadkygdmstekeqvdeaIELKVGHMVDINSGDYSGLSGQISRIIDNDEYI-VDV 247
Cdd:TIGR00922 103 ILNAL------EEGKDKPKPK----------------------IDFEVGEQVRVNDGPFANFTGTVEEVDYEKSKLkVSV 154

                         250
                  ....*....|....*..
gi 400275803  248 QILGKLVSVKLNKKQLK 264
Cdd:TIGR00922 155 SIFGRETPVELEFSQVE 171
NGN cd08000
N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization ...
 95-144 4.83e-04

N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization Substance G (NusG) and its eukaryotic homolog Spt5 are involved in transcription elongation and termination. NusG contains an NGN domain at its N-terminus and Kyrpides Ouzounis and Woese (KOW) repeats at its C-terminus in bacteria and archaea. The eukaryotic ortholog, Spt5, is a large protein composed of an acidic N-terminus, an NGN domain, and multiple KOW motifs at its C-terminus. Spt5 forms a Spt4-Spt5 complex that is an essential RNA Polymerase II elongation factor. NusG was originally discovered as an N-dependent antitermination enhancing activity in Escherichia coli and has a variety of functions, such as being involved in RNA polymerase elongation and Rho-termination in bacteria. Orthologs of the NusG gene exist in all bacteria, but its functions and requirements are different. The diverse activities suggest that, after diverging from a common ancestor, NusG proteins became specialized in different bacteria.


Pssm-ID: 193574 [Multi-domain]  Cd Length: 99  Bit Score: 38.45  E-value: 4.83e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 400275803  95 YPGYIYIKMEMNEAAWFAVRNTVNITGIVGSSGkgaKPIPISSSEELDLL 144
Cdd:cd08000   53 FPGYVFVETDLSPELYELIREVPGVIGILGNGE---EPSPVSDEEIEMIL 99
NGN_SP_RfaH cd09892
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), RfaH; ...
 96-136 2.20e-03

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), RfaH; RfaH is an operon-specific virulence regulator, thought to have arisen from an early duplication of N-Utilization Substance G (NusG). Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. In contrast, RfaH is a non-essential protein that controls expression of operons containing an ops (operon polarity suppressor) element in their transcribed DNA. RfaH and NusG are different in their response to Rho-dependent terminators and regulatory targets. The NusG N-terminal (NGN) domain is quite similar in all NusG orthologs, but its C-terminal domains and the linker that separate these two domains are different. The domain organization of NusG and its homologs suggest that the common properties of NusG and RfaH are due to their similar NGN domains.


Pssm-ID: 193581 [Multi-domain]  Cd Length: 96  Bit Score: 36.38  E-value: 2.20e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 400275803  96 PGYIYIKMEMNEAAWFAVRNTVNITGIVGSsgkGAKPIPIS 136
Cdd:cd09892   50 PGYLFVRLDPEVQNWRPIRSTRGVSRLVRF---GGEPAPVP 87
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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