RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to GDP. The Rho family of small G proteins, which includes Cdc42Hs, activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. G proteins generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude. The RhoGAPs are one of the major classes of regulators of Rho G proteins.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  65 PVILLLLQSRLYdQGGLQAEGVFRITGENSEEEFVREQLNKGIIPD---GIDVHCLAGLIKAWFRELPRGVLDPLPSEQV 141
Cdd:cd00159    1 PLIIEKCIEYLE-KNGLNTEGIFRVSGSASKIEELKKKFDRGEDIDdleDYDVHDVASLLKLYLRELPEPLIPFELYDEF 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 142 MQCESDED-------FVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNMSQMADPLTALM----YA 210
Cdd:cd00159   80 IELAKIEDeeerieaLKELLKSLPPENRDLLKYLLKLLHKISQNSEVNKMTASNLAIVFAPTLLRPPDSDDELLedikKL 159

                 ....*..
gi 332190157 211 VQVMKLL 217
Cdd:cd00159  160 NEIVEFL 166

RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to GDP. The Rho family of small G proteins, which includes Cdc42Hs, activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. G proteins generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude. The RhoGAPs are one of the major classes of regulators of Rho G proteins.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  65 PVILLLLQSRLYdQGGLQAEGVFRITGENSEEEFVREQLNKGIIPD---GIDVHCLAGLIKAWFRELPRGVLDPLPSEQV 141
Cdd:cd00159    1 PLIIEKCIEYLE-KNGLNTEGIFRVSGSASKIEELKKKFDRGEDIDdleDYDVHDVASLLKLYLRELPEPLIPFELYDEF 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 142 MQCESDED-------FVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNMSQMADPLTALM----YA 210
Cdd:cd00159   80 IELAKIEDeeerieaLKELLKSLPPENRDLLKYLLKLLHKISQNSEVNKMTASNLAIVFAPTLLRPPDSDDELLedikKL 159

                 ....*..
gi 332190157 211 VQVMKLL 217
Cdd:cd00159  160 NEIVEFL 166

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157   80 GLQAEGVFRITGENSEEEFVREQLNKGIIPD----GIDVHCLAGLIKAWFRELPrgvlDPL-PSEQ------VMQCESDE 148
Cdd:pfam00620  15 GLDTEGIFRVSGSASRIKELREAFDRGPDVDldleEEDVHVVASLLKLFLRELP----EPLlTFELyeefieAAKLPDEE 90

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 332190157  149 DFV----KVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNM 197
Cdd:pfam00620  91 ERLealrELLRKLPPANRDTLRYLLAHLNRVAQNSDVNKMNAHNLAIVFGPTL 143

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 332190157   1 MDIGGPTNIRHVAHVTFDRFDGF-LGLPSEFEPDVPRKAPSA 41
Cdd:cd00132    1 MEISTPTDFKHISHVGWDGVGFDgANLPPDLQSLFQTAGISA 42

RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to GDP. The Rho family of small G proteins, which includes Cdc42Hs, activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. G proteins generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude. The RhoGAPs are one of the major classes of regulators of Rho G proteins.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  65 PVILLLLQSRLYdQGGLQAEGVFRITGENSEEEFVREQLNKGIIPD---GIDVHCLAGLIKAWFRELPRGVLDPLPSEQV 141
Cdd:cd00159    1 PLIIEKCIEYLE-KNGLNTEGIFRVSGSASKIEELKKKFDRGEDIDdleDYDVHDVASLLKLYLRELPEPLIPFELYDEF 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 142 MQCESDED-------FVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNMSQMADPLTALM----YA 210
Cdd:cd00159   80 IELAKIEDeeerieaLKELLKSLPPENRDLLKYLLKLLHKISQNSEVNKMTASNLAIVFAPTLLRPPDSDDELLedikKL 159

                 ....*..
gi 332190157 211 VQVMKLL 217
Cdd:cd00159  160 NEIVEFL 166

RhoGAP_KIAA1688: GTPase-activator protein (GAP) domain for Rho-like GTPases found in KIAA1688-like proteins; KIAA1688 is a protein of unknown function that contains a RhoGAP domain and a myosin tail homology 4 (MyTH4) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  64 VPVILLLLQSRLYDQGGLQAEGVFRITGENSEEEFVREQLNKGIIPD-GI-DVHCLAGLIKAWFRELPrgvlDPL-PSEQ 140
Cdd:cd04389   21 LPWILTFLSEKVLALGGFQTEGIFRVPGDIDEVNELKLRVDQWDYPLsGLeDPHVPASLLKLWLRELE----EPLiPDAL 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 141 VMQC----ESDEDFVKVVRLLPQTEASLLNWAINLMADVIQFEHV--NKMNSRNLALVFAPNM--SQMADPLTALMYAVQ 212
Cdd:cd04389   97 YQQCisasEDPDKAVEIVQKLPIINRLVLCYLINFLQVFAQPENVahTKMDVSNLAMVFAPNIlrCTSDDPRVIFENTRK 176

                        170
                 ....*....|
gi 332190157 213 VMKLLKSLTE 222
Cdd:cd04389  177 EMSFLRTLIE 186

RhoGAP_FAM13A1a: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of FAM13A1, isoform a-like proteins. The function of FAM13A1a is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by up several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  45 VFGVSTESMQLSYDSRgNCVPVILLLLQSRLyDQGGLQAEGVFRITGENSEEEFVREQLNKGIIPD---GIDVHCLAGLI 121
Cdd:cd04393    2 VFGVPLQELQQAGQPE-NGVPAVVRHIVEYL-EQHGLEQEGLFRVNGNAETVEWLRQRLDSGEEVDlskEADVCSAASLL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 122 KAWFRELPRGVLDPLPSEQVMQCESD--------EDFVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVF 193
Cdd:cd04393   80 RLFLQELPEGLIPASLQIRLMQLYQDyngedefgRKLRDLLQQLPPVNYSLLKFLCHFLSNVASQHHENRMTAENLAAVF 159

                 ...
gi 332190157 194 APN 196
Cdd:cd04393  160 GPD 162

RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for Rac1 and Cdc42. Short isoforms (without the PH domain) of ARHGAP24, called RC-GAP72 and p73RhoGAP, and of ARHGAP22, called p68RacGAP, has been shown to be involved in angiogenesis and endothelial cell capillary formation. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  80 GLQAEGVFRITGENSEEEFVREQLNKGIIP--DG-IDVHCLAGLIKAWFRELPRGVL------DPLPSEQVM---QCESD 147
Cdd:cd04390   37 GLKEEGLFRLPGQANLVKQLQDAFDAGERPsfDSdTDVHTVASLLKLYLRELPEPVIpwaqyeDFLSCAQLLskdEEKGL 116

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 332190157 148 EDFVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNM--SQMADPLTALMYAVQVMKLL 217
Cdd:cd04390  117 GELMKQVSILPKVNYNLLSYICRFLDEVQSNSSVNKMSVQNLATVFGPNIlrPKVEDPATIMEGTPQIQQLM 188

RhoGAP_ARHGAP20: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP20-like proteins. ArhGAP20, also known as KIAA1391 and RA-RhoGAP, contains a RhoGAP, a RA, and a PH domain, and ANXL repeats. ArhGAP20 is activated by Rap1 and induces inactivation of Rho, which in turn leads to neurite outgrowth. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  67 ILLLLQsrlydQGGLQAEGVFRITGENSEEEFVREQLNKGIIPD--GIDVHCLAGLIKAWFRELPRGVLDPLPSEQ---V 141
Cdd:cd04402   22 MLSLLY-----QKGPSTEGIFRRSANAKACKELKEKLNSGVEVDlkAEPVLLLASVLKDFLRNIPGSLLSSDLYEEwmsA 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 142 MQCESDEDFVKVVR----LLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNMSQMADPLTALMyavQVMKLL 217
Cdd:cd04402   97 LDQENEEEKIAELQrlldKLPRPNVLLLKHLICVLHNISQNSETNKMDAFNLAVCIAPSLLWPPASSELQN---EDLKKV 173

                 ....*
gi 332190157 218 KSLTE 222
Cdd:cd04402  174 TSLVQ 178

RhoGAP-ARHGAP11A: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP11A-like proteins. The mouse homolog of human ArhGAP11A has been detected as a gene exclusively expressed in immature ganglion cells, potentially playing a role in retinal development. The exact function of ArhGAP11A is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  45 VFGVSTESMQLSYDSRGNCVPVILLLLQSRLYDQggLQAEGVFRITGENSEEEFVREQLNKGIIPDG----IDVhclAGL 120
Cdd:cd04394    1 VFGVPLHSLPHSTVPEYGNVPKFLVDACTFLLDH--LSTEGLFRKSGSVVRQKELKAKLEGGEACLSsalpCDV---AGL 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 121 IKAWFRELPRGVLDPLPSEQVMQC-------ESDEDFVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVF 193
Cdd:cd04394   76 LKQFFRELPEPLLPYDLHEALLKAqelptdeERKSATLLLTCLLPDEHVNTLRYFFSFLYDVAQRCSENKMDSSNLAVIF 155

                 ....*....
gi 332190157 194 APNMSQMAD 202
Cdd:cd04394  156 APNLFQSEE 164

RhoGAP_ARAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in ARAPs. ARAPs (also known as centaurin deltas) contain, besides the RhoGAP domain, an Arf GAP, ankyrin repeat ras-associating, and PH domains. Since their ArfGAP activity is PIP3-dependent, ARAPs are considered integration points for phosphoinositide, Arf and Rho signaling. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  47 GVSTESMQLSydsrGNCVPVILLLLQSRLYdQGGLQAEGVFRITGENSE-----EEFVREQLNKGIIPDGIDVHCLAGLI 121
Cdd:cd04385    2 GPALEDQQLT----DNDIPVIVDKCIDFIT-QHGLMSEGIYRKNGKNSSvkkllEAFRKDARSVQLREGEYTVHDVADVL 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 122 KAWFRELPRGVLDPLP-------SEQVMQCESDEDFVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFA 194
Cdd:cd04385   77 KRFLRDLPDPLLTSELhaewieaAELENKDERIARYKELIRRLPPINRATLKVLIGHLYRVQKHSDENQMSVHNLALVFG 156

                        170       180
                 ....*....|....*....|.
gi 332190157 195 PN-MSQMADPLTALMYAVQVM 214
Cdd:cd04385  157 PTlFQTDEHSVGQTSHEVKVI 177

RhoGAP_myosin_IX: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in class IX myosins. Class IX myosins contain a characteristic head domain, a neck domain, a tail domain which contains a C6H2-zinc binding motif and a RhoGAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  46 FGVSTESMQlsydSRGNCVPVILLLLqSRLYDQGGLQAEGVFRITGENSEEEFVREQLNKGiiPDGID-----VHCLAGL 120
Cdd:cd04377    1 FGVSLSSLT----SEDRSVPLVLEKL-LEHIEMHGLYTEGIYRKSGSANKIKELRQGLDTD--PDSVNledypIHVITSV 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 121 IKAWFRELPrgvlDPLpseqvMQCESDEDFVKVVRL----------------LPQTEASLLNWAINLMADVIQFEHVNKM 184
Cdd:cd04377   74 LKQWLRELP----EPL-----MTFELYENFLRAMELeekqervralysvleqLPRANLNTLERLIFHLVRVALQEEVNRM 144

                        170       180
                 ....*....|....*....|....*.
gi 332190157 185 NSRNLALVFAPNM---SQMADPLTAL 207
Cdd:cd04377  145 SANALAIVFAPCIlrcPDTADPLQSL 170

RhoGAP_chimaerin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of chimaerins. Chimaerins are a family of phorbolester- and diacylglycerol-responsive GAPs specific for the Rho-like GTPase Rac. Chimaerins exist in two alternative splice forms that each contain a C-terminal GAP domain, and a central C1 domain which binds phorbol esters, inducing a conformational change that activates the protein; one splice form is lacking the N-terminal Src homology-2 (SH2) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  80 GLQAEGVFRITGENSEEEFVREQLNKgiipDG----------IDVHCLAGLIKAWFRELPRGVL--DPLPS-----EQVM 142
Cdd:cd04372   31 GLQSEGLYRVSGFAEEIEDVKMAFDR----DGekadisatvyPDINVITGALKLYFRDLPIPVItyDTYPKfidaaKISN 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 143 QCESDEDFVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNM-----SQMADPLTALMYAVQVMKLL 217
Cdd:cd04372  107 PDERLEAVHEALMLLPPAHYETLRYLMEHLKRVTLHEKDNKMNAENLGIVFGPTLmrppeDSALTTLNDMRYQILIVQLL 186

RhoGAP_ARHGAP19: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP19-like proteins. The function of ArhGAP19 is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  67 ILLLLQSRLYDQGGLQAEGVFRITGENSEEEFVREQLNKGIIPD----GIDVHCLAGLIKAWFRELPrgvlDPLPSE--- 139
Cdd:cd04392   10 IAQIYQLIEYLEKNLRVEGLFRKPGNSARQQELRDLLNSGTDLDlesgGFHAHDCATVLKGFLGELP----EPLLTHahy 85

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 332190157 140 ----QVMQ-CESDEDFVK---------------VVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNM 197
Cdd:cd04392   86 pahlQIADlCQFDEKGNKtsapdkerllealqlLLLLLPEENRNLLKLILDLLYQTAKHEDKNKMSADNLALLFTPHL 163

RhoGAP_srGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an N-terminal FCH domain, a central RhoGAP domain and a C-terminal SH3 domain; this SH3 domain interacts with the intracellular proline-rich-tail of the Roundabout receptor (Robo). This interaction with Robo then activates the rhoGAP domain which in turn inhibits Cdc42 activity. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  66 VILLLLQS--RLYDQGGLQAEGVFRITGENSEEEFVREQLNKGIIP-----DGIDVHCLAGLIKAWFRELPRGVLDPLPS 138
Cdd:cd04383   17 AIPLVVESciRFINLYGLQHQGIFRVSGSQVEVNDIKNAFERGEDPladdqNDHDINSVAGVLKLYFRGLENPLFPKERF 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 139 EQVMQC---ESDEDFV----KVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNMSQMADPLTALMYAV 211
Cdd:cd04383   97 EDLMSCvklENPTERVhqirEILSTLPRSVIIVMRYLFAFLNHLSQFSDENMMDPYNLAICFGPTLMPVPEGQDQVSCQA 176

                 ....*....
gi 332190157 212 QVMKLLKSL 220
Cdd:cd04383  177 HVNELIKTI 185

RhoGAP_p190: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an N-terminal GTPase domain and a C-terminal GAP domain. The central portion of p190 contains important regulatory phosphorylation sites. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  77 DQGGLQAEGVFRITGENSEEEFVREQL----NKGIIPDGIDVHCLAGLIKAWFRELPrgvlDPLPS-------EQVMQCE 145
Cdd:cd04373   27 EATGLETEGIYRVSGNKTHLDSLQKQFdqdhNLDLVSKDFTVNAVAGALKSFFSELP----DPLIPysmhlelVEAAKIN 102

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 332190157 146 SDE---DFVK-VVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNM 197
Cdd:cd04373  103 DREqrlHALKeLLKKFPPENFDVFKYVITHLNKVSQNSKVNLMTSENLSICFWPTL 158

RhoGAP_DLC1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of DLC1-like proteins. DLC1 shows in vitro GAP activity towards RhoA and CDC42. Beside its C-terminal GAP domain, DLC1 also contains a SAM (sterile alpha motif) and a START (StAR-related lipid transfer action) domain. DLC1 has tumor suppressor activity in cell culture. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  44 TVFGVS-TESMQLSYDSRGNCVPVILLLLQSRLYDQGGLqaegvFRITGENSEEEFVR---EQLNKGIIPDGIDVHCLAG 119
Cdd:cd04375    3 NVFGVPlLVNLQRTGQPLPRSIQQAMRWLRNNALDQVGL-----FRKSGVKSRIQKLRsmiESSTDNVNYDGQQAYDVAD 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 120 LIKAWFRELPRGVLDPLPSE------QVMQCESDEDFVK-VVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALV 192
Cdd:cd04375   78 MLKQYFRDLPEPLLTNKLSEtfiaifQYVPKEQRLEAVQcAILLLPDENREVLQTLLYFLSDVAANSQENQMTATNLAVC 157

                 ....*
gi 332190157 193 FAPNM 197
Cdd:cd04375  158 LAPSL 162

RhoGAP_myosin_IXA: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXA. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  46 FGVstESMQLSYDSRGncVPVILLLLQSRLyDQGGLQAEGVFRITGENSEEEFVREQLNKGIIP---DGIDVHCLAGLIK 122
Cdd:cd04406    1 FGV--ELSRLTSEDRS--VPLVVEKLINYI-EMHGLYTEGIYRKSGSTNKIKELRQGLDTDANSvnlDDYNIHVIASVFK 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 123 AWFRELPRGVLDPLPSEQVMQCESDEDFVKVVR-------LLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAP 195
Cdd:cd04406   76 QWLRDLPNPLMTFELYEEFLRAMGLQERRETVRgvysvidQLSRTHLNTLERLIFHLVRIALQEETNRMSANALAIVFAP 155

                        170
                 ....*....|....*
gi 332190157 196 NM---SQMADPLTAL 207
Cdd:cd04406  156 CIlrcPDTTDPLQSV 170

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).

                           10        20        30
                   ....*....|....*....|....*....|....*.
gi 332190157     3 IGGPTNIRHVAHVTFD-RFDGFLGLPSEFEPDVPRK 37
Cdd:smart00285   1 ISTPTNFKHIAHVGFDgQTGGFTGLPTEWKSLLKTS 36

RhoGAP_SYD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in SYD-1_like proteins. Syd-1, first identified and best studied in C.elegans, has been shown to play an important role in neuronal development by specifying axonal properties. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  46 FGVSTESMQLSYDSRGNcVPVILLLLQSRLyDQGGLQAEGVFRITGENSEEEFVREQ---------LNKGIIPDgidVHC 116
Cdd:cd04379    1 FGVPLSRLVEREGESRD-VPIVLQKCVQEI-ERRGLDVIGLYRLCGSAAKKKELRDAfernsaaveLSEELYPD---INV 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 117 LAGLIKAWFRELPRGVLDPLPSEQVMQC----------ESDEDFVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNS 186
Cdd:cd04379   76 ITGVLKDYLRELPEPLITPQLYEMVLEAlavalpndvqTNTHLTLSIIDCLPLSAKATLLLLLDHLSLVLSNSERNKMTP 155

                 ....*....
gi 332190157 187 RNLALVFAP 195
Cdd:cd04379  156 QNLAVCFGP 164

RhoGAP_fSAC7_BAG7: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal SAC7 and BAG7-like proteins. Both proteins are GTPase activating proteins of Rho1, but differ functionally in vivo: SAC7, but not BAG7, is involved in the control of Rho1-mediated activation of the PKC-MPK1 pathway. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  41 ASATVFGVSTESMQLSYDSrgncVPVILLllQSRLY-DQGGLQAEGVFRITGENSEeefVRE-QL--------NKGIIPD 110
Cdd:cd04396   13 ASVAISIVDEDGEQYVYGY----IPVVVA--KCGVYlKENATEVEGIFRVAGSSKR---IRElQLifstppdyGKSFDWD 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 111 GIDVHCLAGLIKAW----------------FREL----PRGVLDPLPSEQVMQCESDEDFVK----VVRLLPQTEASLLN 166
Cdd:cd04396   84 GYTVHDAASVLRRYlnnlpeplvpldlyeeFRNPlrkrPRILQYMKGRINEPLNTDIDQAIKeyrdLITRLPNLNRQLLL 163

                        170       180       190
                 ....*....|....*....|....*....|.
gi 332190157 167 WAINLMADVIQFEHVNKMNSRNLALVFAPNM 197
Cdd:cd04396  164 YLLDLLAVFARNSDKNLMTASNLAAIFQPGI 194

RhoGAP_fLRG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal LRG1-like proteins. Yeast Lrg1p is required for efficient cell fusion, and mother-daughter cell separation, possibly through acting as a RhoGAP specifically regulating 1,3-beta-glucan synthesis. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157  78 QGGLQAEGVFRITGENSEEEFVREQLNKG--IIPD--GIDVHCLAGLIKAWFRELPrgvlDPLPSEQ------VMQCESD 147
Cdd:cd04397   40 QMDMSVEGVFRKNGNIRRLKELTEEIDKNptEVPDlsKENPVQLAALLKKFLRELP----DPLLTFKlyrlwiSSQKIED 115

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 332190157 148 EDFVK-----VVRLLPQ-----TEA--SLLNWainlmadVIQFEHV-----NKMNSRNLALVFAPNM--SQMADPLTA-- 206
Cdd:cd04397  116 EEERKrvlhlVYCLLPKyhrdtMEVlfSFLKW-------VSSFSHIdeetgSKMDIHNLATVITPNIlySKTDNPNTGde 188

                        170
                 ....*....|....*..
gi 332190157 207 LMYAVQVMKLLKSLTEK 223
Cdd:cd04397  189 YFLAIEAVNYLIENNEE 205