P-loop containing Nucleoside Triphosphate Hydrolases; Members of the P-loop NTPase domain superfamily are characterized by a conserved nucleotide phosphate-binding motif, also referred to as the Walker A motif (GxxxxGK[S/T], where x is any residue), and the Walker B motif (hhhh[D/E], where h is a hydrophobic residue). The Walker A and B motifs bind the beta-gamma phosphate moiety of the bound nucleotide (typically ATP or GTP) and the Mg2+ cation, respectively. The P-loop NTPases are involved in diverse cellular functions, and they can be divided into two major structural classes: the KG (kinase-GTPase) class which includes Ras-like GTPases and its circularly permutated YlqF-like; and the ASCE (additional strand catalytic E) class which includes ATPase Binding Cassette (ABC), DExD/H-like helicases, 4Fe-4S iron sulfur cluster binding proteins of NifH family, RecA-like F1-ATPases, and ATPases Associated with a wide variety of Activities (AAA). Also included are a diverse set of nucleotide/nucleoside kinase families.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRvvSQEEIVRAAKEANIHAFIESLPN 69
Cdd:cd03249   61 LLDGVDIRDLNLRWLRSQIGLVSQEPVLFDGTIAENIRYGKPDA--TDEEVEEAAKKANIHDFIMSLPD 127

ATP-binding cassette domain of a mitochondrial protein MTABC3 and related proteins; MTABC3 (also known as ABCB6) is a mitochondrial ATP-binding cassette protein involved in iron homeostasis and one of four ABC transporters expressed in the mitochondrial inner membrane, the other three being MDL1(ABC7), MDL2, and ATM1. In fact, the yeast MDL1 (multidrug resistance-like protein 1) and MDL2 (multidrug resistance-like protein 2) transporters are also included in this CD. MDL1 is an ATP-dependent permease that acts as a high-copy suppressor of ATM1 and is thought to have a role in resistance to oxidative stress. Interestingly, subfamily B is more closely related to the carboxyl-terminal component of subfamily C than the two halves of ABCC molecules are with one another.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRvvSQEEIVRAAKEANIHAFIESLPN 69
Cdd:cd03249   61 LLDGVDIRDLNLRWLRSQIGLVSQEPVLFDGTIAENIRYGKPDA--TDEEVEEAAKKANIHDFIMSLPD 127

Conjugate Transporter-2 (CT2) Family protein; [Transport and binding proteins, Other]

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073    1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSrvVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:TIGR00958 539 LLDGVPLVQYDHHYLHRQVALVGQEPVLFSGSVRENIAYGLTD--TPDEEIMAAAKAANAHDFIMEFPN 605

ATP-binding cassette domain of a mitochondrial protein MTABC3 and related proteins; MTABC3 (also known as ABCB6) is a mitochondrial ATP-binding cassette protein involved in iron homeostasis and one of four ABC transporters expressed in the mitochondrial inner membrane, the other three being MDL1(ABC7), MDL2, and ATM1. In fact, the yeast MDL1 (multidrug resistance-like protein 1) and MDL2 (multidrug resistance-like protein 2) transporters are also included in this CD. MDL1 is an ATP-dependent permease that acts as a high-copy suppressor of ATM1 and is thought to have a role in resistance to oxidative stress. Interestingly, subfamily B is more closely related to the carboxyl-terminal component of subfamily C than the two halves of ABCC molecules are with one another.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRvvSQEEIVRAAKEANIHAFIESLPN 69
Cdd:cd03249   61 LLDGVDIRDLNLRWLRSQIGLVSQEPVLFDGTIAENIRYGKPDA--TDEEVEEAAKKANIHDFIMSLPD 127

ABC-type bacteriocin/lantibiotic exporters, contain an N-terminal double-glycine peptidase domain [Defense mechanisms];

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSrvVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:COG2274  533 LIDGIDLRQIDPASLRRQIGVVLQDVFLFSGTIRENITLGDPD--ATDEEIIEAARLAGLHDFIEALPM 599

ATP-binding cassette domain of the bacterial lipid flippase and related proteins, subfamily C; MsbA is an essential ABC transporter, closely related to eukaryotic MDR proteins. ABC transporters are a large family of proteins involved in the transport of a wide variety of different compounds, like sugars, ions, peptides, and more complex organic molecules. The nucleotide binding domain shows the highest similarity between all members of the family. ABC transporters are a subset of nucleotide hydrolases that contain a signature motif, Q-loop, and H-loop/switch region, in addition to, the Walker A motif/P-loop and Walker B motif commonly found in a number of ATP- and GTP-binding and hydrolyzing proteins.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDnsRVVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:cd03251   60 LIDGHDVRDYTLASLRRQIGLVSQDVFLFNDTVAENIAYGR--PGATREEVEEAARAANAHEFIMELPE 126

ATP-binding cassette domain of iron-sulfur clusters transporter, subfamily C; ATM1 is an ABC transporter that is expressed in the mitochondria. Although the specific function of ATM1 is unknown, its disruption results in the accumulation of excess mitochondrial iron, loss of mitochondrial cytochromes, oxidative damage to mitochondrial DNA, and decreased levels of cytosolic heme proteins. ABC transporters are a large family of proteins involved in the transport of a wide variety of different compounds, like sugars, ions, peptides, and more complex organic molecules. The nucleotide binding domain shows the highest similarity between all members of the family. ABC transporters are a subset of nucleotide hydrolases that contain a signature motif, Q-loop, and H-loop/switch region, in addition to, the Walker A motif/P-loop and Walker B motif commonly found in a number of ATP- and GTP-binding and hydrolyzing proteins.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSrvVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:cd03253   59 LIDGQDIREVTLDSLRRAIGVVPQDTVLFNDTIGYNIRYGRPD--ATDEEVIEAAKAAQIHDKIMRFPD 125

multidrug resistance protein (mdr1); Provisional

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073    1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSrvVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:PTZ00265 1280 LLDGVDICDYNLKDLRNLFSIVSQEPMLFNMSIYENIKFGKED--ATREDVKRACKFAAIDEFIESLPN 1346

ATP-binding cassette domain of bacteriocin exporters, subfamily C; Many non-lantibiotic bacteriocins of lactic acid bacteria are produced as precursors which have N-terminal leader peptides that share similarities in amino acid sequence and contain a conserved processing site of two glycine residues in positions -1 and -2. A dedicated ATP-binding cassette (ABC) transporter is responsible for the proteolytic cleavage of the leader peptides and subsequent translocation of the bacteriocins across the cytoplasmic membrane.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDnsRVVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:cd03245   62 LLDGTDIRQLDPADLRRNIGYVPQDVTLFYGTLRDNITLGA--PLADDERILRAAELAGVTDFVNKHPN 128

ABC-type protease/lipase transport system, ATPase and permease components [Intracellular trafficking, secretion, and vesicular transport];

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   2 LDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIA-YGDnsrvVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:COG4618  391 LDGADLSQWDREELGRHIGYLPQDVELFDGTIAENIArFGD----ADPEKVVAAAKLAGVHEMILRLPD 455

lipid A ABC transporter ATP-binding protein/permease MsbA;

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRvVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:PRK11176 401 LLDGHDLRDYTLASLRNQVALVSQNVHLFNDTIANNIAYARTEQ-YSREQIEEAARMAYAMDFINKMDN 468

cysteine/glutathione ABC transporter membrane/ATP-binding component; Reviewed

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSrvVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:PRK11174 407 KINGIELRELDPESWRKHLSWVGQNPQLPHGTLRDNVLLGNPD--ASDEQLQQALENAWVSEFLPLLPQ 473

ATP-binding cassette domain 2 of multidrug resistance-associated protein; The ABC subfamily C is also known as MRP (multidrug resistance-associated protein). Some of the MRP members have five additional transmembrane segments in their N-terminus, but the function of these additional membrane-spanning domains is not clear. The MRP was found in the multidrug-resistance lung cancer cell in which p-glycoprotein was not overexpressed. MRP exports glutathione by drug stimulation, as well as, certain substrates in conjugated forms with anions, such as glutathione, glucuronate, and sulfate.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIaygDNSRVVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:cd03244   62 LIDGVDISKIGLHDLRSRISIIPQDPVLFSGTIRSNL---DPFGEYSDEELWQALERVGLKEFVESLPG 127

ATP-binding cassette domain of the phosphate transport system; Phosphate uptake is of fundamental importance in the cell physiology of bacteria because phosphate is required as a nutrient. The Pst system of E. coli comprises four distinct subunits encoded by the pstS, pstA, pstB, and pstC genes. The PstS protein is a phosphate-binding protein located in the periplasmic space. PstA and PstC are hydrophobic and they form the transmembrane portion of the Pst system. PstB is the catalytic subunit, which couples the energy of ATP hydrolysis to the import of phosphate across cellular membranes through the Pst system, often referred as ABC-protein. PstB belongs to one of the largest superfamilies of proteins characterized by a highly conserved adenosine triphosphate (ATP) binding cassette (ABC), which is also a nucleotide binding domain (NBD).

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 330376073   1 LLDGKEI--KRLNVQWLRAHLGIVSQEPILFDCSIAENIAYG 40
Cdd:cd03260   63 LLDGKDIydLDVDVLELRRRVGMVFQKPNPFPGSIYDNVAYG 104

multi drug resistance-associated protein (MRP); This model describes multi drug resistance-associated protein (MRP) in eukaryotes. The multidrug resistance-associated protein is an integral membrane protein that causes multidrug resistance when overexpressed in mammalian cells. It belongs to ABC transporter superfamily. The protein topology and function was experimentally demonstrated by epitope tagging and immunofluorescence. Insertion of tags in the critical regions associated with drug efflux, abrogated its function. The C-terminal domain seem to highly conserved. [Transport and binding proteins, Other]

                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073     1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIaygDNSRVVSQEEIVRAAKEANIHAFIESLPN 69
Cdd:TIGR00957 1344 IIDGLNIAKIGLHDLRFKITIIPQDPVLFSGSLRMNL---DPFSQYSDEEVWWALELAHLKTFVSALPD 1409

ATP-binding cassette domain 2 of the sulfonylurea receptor SUR; The SUR domain 2. The sulfonylurea receptor SUR is an ATP binding cassette (ABC) protein of the ABCC/MRP family. Unlike other ABC proteins, it has no intrinsic transport function, neither active nor passive, but associates with the potassium channel proteins Kir6.1 or Kir6.2 to form the ATP-sensitive potassium (K(ATP)) channel. Within the channel complex, SUR serves as a regulatory subunit that fine-tunes the gating of Kir6.x in response to alterations in cellular metabolism. It constitutes a major pharmaceutical target as it binds numerous drugs, K(ATP) channel openers and blockers, capable of up- or down-regulating channel activity.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIaygDNSRVVSQEEIVRAAKEANIHAFIESLP 68
Cdd:cd03288   79 VIDGIDISKLPLHTLRSRLSIILQDPILFSGSIRFNL---DPECKCTDDRLWEALEIAQLKNMVKSLP 143

ATP-binding cassette domain of the carbohydrate and solute transporters-like; This family is comprised of proteins involved in the transport of apparently unrelated solutes and proteins specific for di- and oligosaccharides and polyols. ABC transporters are a large family of proteins involved in the transport of a wide variety of different compounds, like sugars, ions, peptides and more complex organic molecules. The nucleotide-binding domain shows the highest similarity between all members of the family. ABC transporters are a subset of nucleotide hydrolases that contain a signature motif, Q-loop, and H-loop/switch region, in addition to, the Walker A motif/P-loop and Walker B motif commonly found in a number of ATP- and GTP-binding and hydrolyzing proteins.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQwlRAHLGIVSQEPILFD-CSIAENIAYGDNSRVVSQEEIVRAAKEA 58
Cdd:cd03259   58 LIDGRDVTGVPPE--RRNIGMVFQDYALFPhLTVAENIAFGLKLRGVPKAEIRARVREL 114

ABC-type Fe3+/spermidine/putrescine transport systems, ATPase component [Amino acid transport and metabolism];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 330376073   1 LLDGKEIKRLNVQwlRAHLGIVSQEPILF-DCSIAENIAYGDNSRVVSQEEIVRAAKEA 58
Cdd:COG3842   63 LLDGRDVTGLPPE--KRNVGMVFQDYALFpHLTVAENVAFGLRMRGVPKAEIRARVAEL 119

ATP-binding cassette domain 2 of NFT1, subfamily C; Domain 2 of NFT1 (New full-length MRP-type transporter 1). NFT1 belongs to the MRP (multidrug resistance-associated protein) family of ABC transporters. Some of the MRP members have five additional transmembrane segments in their N-terminus, but the function of these additional membrane-spanning domains is not clear. The MRP was found in the multidrug-resisting lung cancer cell in which p-glycoprotein was not overexpressed. MRP exports glutathione by drug stimulation, as well as, certain substrates in conjugated forms with anions such as glutathione, glucuronate, and sulfate.

                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENI 37
Cdd:cd03369   66 EIDGIDISTIPLEDLRSSLTIIPQDPTLFSGTIRSNL 102

cobalt transporter ATP-binding subunit; Provisional

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 330376073   4 GKEIKRLNVqwLRAHLGIVSQ--EPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEA 58
Cdd:PRK13634  74 GKKNKKLKP--LRKKVGIVFQfpEHQLFEETVEKDICFGPMNFGVSEEDAKQKAREM 128

phosphate ABC transporter ATP-binding protein; Provisional

                         10        20        30
                 ....*....|....*....|....*....|...
gi 330376073   8 KRLNVQWLRAHLGIVSQEPILFDCSIAENIAYG 40
Cdd:PRK14258  79 RRVNLNRLRRQVSMVHPKPNLFPMSVYDNVAYG 111

ABC-type glutathione transport system ATPase component, contains duplicated ATPase domain [Posttranslational modification, protein turnover, chaperones];

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 330376073   1 LLDGKEIKRLNVQWLRAHLGIVSQEPI--LFDCSIAENIAYGDNSRVVSQEEIVRAAKEA 58
Cdd:COG1123   67 LLDGRDLLELSEALRGRRIGMVFQDPMtqLNPVTVGDQIAEALENLGLSRAEARARVLEL 126