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Conserved domains on  [gi|32263325|gb|AAP78370|]
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conserved hypothetical protein [Helicobacter hepaticus ATCC 51449]

Protein Classification

NAD(P)H-dependent oxidoreductase( domain architecture ID 10114874)

NAD(P)H-dependent oxidoreductase, similar to nitroreductase NfsB, which catalyzes the reduction of flavin or nitrocompounds using NAD(P)H as electron donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 3-203 1.12e-64

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


:

Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 196.70  E-value: 1.12e-64
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   3 PFLESIYFRHSCKLFDENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPL-CWDQPQITSASEVVVFK 81
Cdd:cd02149   1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAaWFNQPQIKDASHVVVFL 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  82 SLQsdlvppseyakrntfrrkmdlngyetfcsrlgdylkargftdekiaHWSALQAYIMATYMVSYASYIKIDTCYIEGF 161
Cdd:cd02149  81 AKK----------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIEGF 114

                       170       180       190       200
                ....*....|....*....|....*....|....*....|..
gi 32263325 162 EKRKVEELYGLDRFKEQVSLIVCFGYRGKAQQARYRITLDEL 203
Cdd:cd02149 115 DPAKLDEILGLDEKGYKISVMVAFGYRSEEKLPKSRKPLEDV 156
 
Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 3-203 1.12e-64

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 196.70  E-value: 1.12e-64
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   3 PFLESIYFRHSCKLFDENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPL-CWDQPQITSASEVVVFK 81
Cdd:cd02149   1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAaWFNQPQIKDASHVVVFL 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  82 SLQsdlvppseyakrntfrrkmdlngyetfcsrlgdylkargftdekiaHWSALQAYIMATYMVSYASYIKIDTCYIEGF 161
Cdd:cd02149  81 AKK----------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIEGF 114

                       170       180       190       200
                ....*....|....*....|....*....|....*....|..
gi 32263325 162 EKRKVEELYGLDRFKEQVSLIVCFGYRGKAQQARYRITLDEL 203
Cdd:cd02149 115 DPAKLDEILGLDEKGYKISVMVAFGYRSEEKLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 4-204 3.83e-35

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 121.88  E-value: 3.83e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   4 FLESIYFRHSCKLFDeNKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWD--QPQITSASEVVVfk 81
Cdd:COG0778   1 LLELLLTRRSVRKFT-DKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALAEanQEWVADAPVLIV-- 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  82 slqsdlvppseyakrntfrrkmdlngyetFCSRlgdylkaRGFTDEKIAHWSALQAYIMATYMVSYASYIKIDTCYIEGF 161
Cdd:COG0778  78 -----------------------------VCAD-------PDRSEKVPERYALLDAGIAAQNLLLAARALGLGTCWIGGF 121

                       170       180       190       200
                ....*....|....*....|....*....|....*....|...
gi 32263325 162 EKRKVEELYGLDRfKEQVSLIVCFGYRGKAQQARYRITLDELV 204
Cdd:COG0778 122 DPEKVRELLGLPE-GEEPVALLALGYPAEELNPRPRKPLEEVV 163
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 8-187 1.17e-34

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 120.57  E-value: 1.17e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325     8 IYFRHSCKLFDeNKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWDQPQITSASEVVVFKslqsdl 87
Cdd:pfam00881   1 IRQRRSVRKFD-PEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLL------ 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325    88 vppSEYAKRNTFRRKMDLNGYETFCSRlGDYLKARGFTDEKIAHWSALQAYIMATYMVSYASYIKIDTCYIEGFEKRKVE 167
Cdd:pfam00881  74 ---RRDANLKLLLQDFLRGAPVLIVIT-ASLSTYLRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAAAVR 149

                         170       180
                  ....*....|....*....|
gi 32263325   168 ELYGLDRfKEQVSLIVCFGY 187
Cdd:pfam00881 150 ELLGLPD-DERLVGLIAVGY 168
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
11-189 1.96e-17

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 76.93  E-value: 1.96e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   11 RHSCKLFDENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAK----KALRP-LCWDQPQITSASEVVVF--KSL 83
Cdd:PRK11053  10 RYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKariaKAAAGnYAFNERKILDASHVVVFcaKTD 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   84 QSD-----LVP--------PSEYAK---RNTFRRKMDLNGYEtfcsrLGDylkargftdekIAHWSALQAYIMATYMVSY 147
Cdd:PRK11053  90 MDDaylelVLEqedadgrfATEEAKaaqDKGRRFFADMHRKE-----LKD-----------LQHWMEKQVYLALGNLLLG 153

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 32263325  148 ASYIKIDTCYIEGFEKRKVEELYGLdrfKEQ---VSLIVCFGYRG 189
Cdd:PRK11053 154 AAALGIDATPIEGFDAAILDAEFGL---REKgltSSVVVPLGYHS 195
 
Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 3-203 1.12e-64

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 196.70  E-value: 1.12e-64
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   3 PFLESIYFRHSCKLFDENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPL-CWDQPQITSASEVVVFK 81
Cdd:cd02149   1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAaWFNQPQIKDASHVVVFL 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  82 SLQsdlvppseyakrntfrrkmdlngyetfcsrlgdylkargftdekiaHWSALQAYIMATYMVSYASYIKIDTCYIEGF 161
Cdd:cd02149  81 AKK----------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIEGF 114

                       170       180       190       200
                ....*....|....*....|....*....|....*....|..
gi 32263325 162 EKRKVEELYGLDRFKEQVSLIVCFGYRGKAQQARYRITLDEL 203
Cdd:cd02149 115 DPAKLDEILGLDEKGYKISVMVAFGYRSEEKLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 4-204 3.83e-35

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 121.88  E-value: 3.83e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   4 FLESIYFRHSCKLFDeNKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWD--QPQITSASEVVVfk 81
Cdd:COG0778   1 LLELLLTRRSVRKFT-DKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALAEanQEWVADAPVLIV-- 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  82 slqsdlvppseyakrntfrrkmdlngyetFCSRlgdylkaRGFTDEKIAHWSALQAYIMATYMVSYASYIKIDTCYIEGF 161
Cdd:COG0778  78 -----------------------------VCAD-------PDRSEKVPERYALLDAGIAAQNLLLAARALGLGTCWIGGF 121

                       170       180       190       200
                ....*....|....*....|....*....|....*....|...
gi 32263325 162 EKRKVEELYGLDRfKEQVSLIVCFGYRGKAQQARYRITLDELV 204
Cdd:COG0778 122 DPEKVRELLGLPE-GEEPVALLALGYPAEELNPRPRKPLEEVV 163
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 8-187 1.17e-34

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 120.57  E-value: 1.17e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325     8 IYFRHSCKLFDeNKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWDQPQITSASEVVVFKslqsdl 87
Cdd:pfam00881   1 IRQRRSVRKFD-PEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLL------ 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325    88 vppSEYAKRNTFRRKMDLNGYETFCSRlGDYLKARGFTDEKIAHWSALQAYIMATYMVSYASYIKIDTCYIEGFEKRKVE 167
Cdd:pfam00881  74 ---RRDANLKLLLQDFLRGAPVLIVIT-ASLSTYLRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAAAVR 149

                         170       180
                  ....*....|....*....|
gi 32263325   168 ELYGLDRfKEQVSLIVCFGY 187
Cdd:pfam00881 150 ELLGLPD-DERLVGLIAVGY 168
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 5-203 3.14e-28

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 103.47  E-value: 3.14e-28
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   5 LESIYFRHSCKLFDENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWDQPQITSASEVVVFkslq 84
Cdd:cd02137   1 LEVIKSRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAYNQPQVTTASAVILV---- 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  85 sdlvppseyakrntfrrkmdlngyetfcsrlgdylkargFTDEKIAhWSALQAYIMATYMvsyasyiKIDTCYIEGFEKR 164
Cdd:cd02137  77 ---------------------------------------LGDLNAG-LAAMNLMLAAKAK-------GYDTCPMGGFDKE 109

                       170       180       190
                ....*....|....*....|....*....|....*....
gi 32263325 165 KVEELYGLDRfKEQVSLIVCFGYRGKAQQARYRITLDEL 203
Cdd:cd02137 110 KVAELLNLPD-RYVPVLLIAIGKAADKAPRSGRLPVDEV 147
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
11-189 1.96e-17

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 76.93  E-value: 1.96e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   11 RHSCKLFDENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAK----KALRP-LCWDQPQITSASEVVVF--KSL 83
Cdd:PRK11053  10 RYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKariaKAAAGnYAFNERKILDASHVVVFcaKTD 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   84 QSD-----LVP--------PSEYAK---RNTFRRKMDLNGYEtfcsrLGDylkargftdekIAHWSALQAYIMATYMVSY 147
Cdd:PRK11053  90 MDDaylelVLEqedadgrfATEEAKaaqDKGRRFFADMHRKE-----LKD-----------LQHWMEKQVYLALGNLLLG 153

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 32263325  148 ASYIKIDTCYIEGFEKRKVEELYGLdrfKEQ---VSLIVCFGYRG 189
Cdd:PRK11053 154 AAALGIDATPIEGFDAAILDAEFGL---REKgltSSVVVPLGYHS 195
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 6-201 4.32e-17

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 75.43  E-value: 4.32e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   6 ESIYFRHSCKLFdENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWDQPQITSASEVVVFKS--- 82
Cdd:cd03370   3 EAIESRRSIRKY-TQEPVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAAYGQAQVTSAPAVIVIYSdme 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  83 --LQ--SDLVPP--SEYAKRNTFrrkmdlngyETFCSRLGDYlkargfTDEKIAHWSALQAYIMATYMVSYASYIKIDTC 156
Cdd:cd03370  82 daLAnlEETIHPglSEERRQREA---------AGLRGAFGKM------SVEQRGQWGLAQANIALGFLLLAAQSLGYDTS 146

                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*..
gi 32263325 157 YIEGFEKRKVEELYGLdrfKEQVSL--IVCFGYRGKAQQARYRITLD 201
Cdd:cd03370 147 PMLGFDPEKVKALLGL---PEHVTIaaLVALGKPAEEGYPHHRHSLE 190
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 11-187 5.93e-16

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 71.17  E-value: 5.93e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  11 RHSCKLFDeNKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPL-CWDQPQITSASEVVVfkslqsdlvp 89
Cdd:cd02062   4 RRSIRKFT-DKPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLAKLaAPNQKFIAGAPVVIV---------- 72

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  90 pseyakrntfrrkmdlngyetFCSRlgdylkargftDEKIAHWSALQAYIMATYMVSYASYIKIDTCYIEGFEKR--KVE 167
Cdd:cd02062  73 ---------------------VVAD-----------PDKSRPWALEDAGAAAQNLLLAAAALGLGSCWIGGFDFRedKVR 120

                       170       180
                ....*....|....*....|
gi 32263325 168 ELYGLDRfKEQVSLIVCFGY 187
Cdd:cd02062 121 ELLGIPE-NLRPVALIAIGY 139
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 4-62 1.57e-11

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 59.71  E-value: 1.57e-11
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 32263325   4 FLESIYFRHSCKLFDEnKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALR 62
Cdd:cd20609   2 FLELAKKRYSVRKFSD-KPVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEEALEKLA 59
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 4-79 4.56e-10

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 55.94  E-value: 4.56e-10
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 32263325   4 FLESIYFRHSCKLFdENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWDQPQITSASEVVV 79
Cdd:cd02139   1 VYEAIKKRRSIRKY-KPTPVEEEKLLRILEAARLAPSAKNRQPWRFIVVKDKELKEKLAEAANGQKFIAEAPVVIV 75
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 8-69 7.69e-08

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 49.89  E-value: 7.69e-08
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 32263325   8 IYFRHSCKLFDeNKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLrSDEAKKALRPLCWDQP 69
Cdd:cd02136   2 IKSRRSVRAFK-DKPVPKETIEKILEAARRAPSGKNTQPWRVYVV-TGKARERLKKAFFGAP 61
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 5-62 2.60e-07

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 48.10  E-value: 2.60e-07
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 32263325   5 LESIYFRHSCKLFDEnKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALR 62
Cdd:cd20608   1 FEAIKTRRSVRRFSD-KPVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSELA 57
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 13-80 3.18e-07

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 49.16  E-value: 3.18e-07
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 32263325  13 SCKLFDeNKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWDQPQITSASEVVVF 80
Cdd:cd02146  10 SVRKFT-DEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELAGNQPYVAQAPVFLVF 76
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 11-61 7.87e-07

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 47.14  E-value: 7.87e-07
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 32263325  11 RHSCKLFdENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKAL 61
Cdd:cd02151   6 RRSIRKY-TDEPIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKL 55
PRK05365 PRK05365
malonic semialdehyde reductase; Provisional
 20-64 3.90e-05

malonic semialdehyde reductase; Provisional


Pssm-ID: 180040 [Multi-domain]  Cd Length: 195  Bit Score: 42.88  E-value: 3.90e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 32263325   20 NKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPL 64
Cdd:PRK05365  24 DEPVSDEQLRELYDLVKWGPTSANCSPARFVFVRSAEAKERLRPA 68
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 11-62 5.29e-05

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 42.15  E-value: 5.29e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|...
gi 32263325  11 RHSCKLFDeNKKIPKETFEEILEVGRLAPSSFGMEPTRLLV-LRSDEAKKALR 62
Cdd:cd02138   5 RWSPRAFS-PEPISEEDLLSLFEAARWAPSCFNEQPWRFVVaRRDTEAFEKLL 56
RutE-like cd02148
nitroreductase similar to Escherichia coli RutE; A subfamily of the nitroreductase family ...
 19-187 8.69e-05

nitroreductase similar to Escherichia coli RutE; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. RutE is involved in the utilization of uracil as the sole nitrogen source; it appears to have the same function as YdfG, which reduces malonic semialdehyde to 3-hydroxypropionic acid.


Pssm-ID: 380323 [Multi-domain]  Cd Length: 186  Bit Score: 41.47  E-value: 8.69e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  19 ENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWD--QPQITSASEVVVFkslqsdlvppseyakr 96
Cdd:cd02148  16 EDKPVSDEELRAIYDLAKWGPTAANCSPARIVFVRSAEAKERLVPHLSEgnREKTMAAPVTAIL---------------- 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325  97 ntfrrKMDLNGYETFcSRL---GDYLKARGFTDEKIAHWSALQ-AYIMATYMVSYASYIKIDTCYIEGFEKRKV-EELYG 171
Cdd:cd02148  80 -----AYDTEFYEHL-PRLfphGDARSWFFGSGPARAEETAFRnASLQAAYFILAARALGLDCGPMSGFDAAGVdAEFFA 153

                       170
                ....*....|....*.
gi 32263325 172 LDRFKeqVSLIVCFGY 187
Cdd:cd02148 154 GTRWK--SNLVVNLGY 167
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
1-80 1.37e-04

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 41.50  E-value: 1.37e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325    1 MNPFLESIYFRHSCKLFDEnKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLCWDQPQITSASEVVVF 80
Cdd:PRK10765   1 MTPTIELILSHRSIRHFTD-EPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELTGGQKYVAQAAEFWVF 79
Frm2-like cd02140
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 4-96 9.02e-04

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. Members of this family are also called NADH dehydrogenase, oxygen-insensitive NAD(P)H nitrogenase or dihydropteridine reductase.


Pssm-ID: 380317  Cd Length: 192  Bit Score: 38.83  E-value: 9.02e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32263325   4 FLESIYFRHSCKLFDENKKIPKETFEEIL-EVGRLAPSSFGMEPTRLLVLRSDEAKKAlrplcWDqpqitsasevvVFKS 82
Cdd:cd02140   1 FLEAAKARRTIYALGKNSPVSDEEIEEIVkEAVKHVPSSFNSQSSRAVILLGDEHKKL-----WD-----------IVKD 64

                        90
                ....*....|....
gi 32263325  83 LQSDLVPPSEYAKR 96
Cdd:cd02140  65 TLRPLVPAEAFEAT 78
nitroreductase cd20610
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 8-61 2.51e-03

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380331 [Multi-domain]  Cd Length: 167  Bit Score: 37.26  E-value: 2.51e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 32263325   8 IYFRHSCKLFdENKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKAL 61
Cdd:cd20610   1 IKKRRSIRKF-KPDPVPKEDIEKILEAANWAPSGMNRQNWEFVVVKGGEKIEKI 53
nitroreductase_FeS-like cd02143
nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family ...
 20-65 3.56e-03

nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family utilize FMN as a cofactor. This family may be involved in the reduction of flavin or nitroaromatic compounds via an obligatory two-electron transfer. Nitroreductase is homodimer. Each subunit contains one FMN molecule.


Pssm-ID: 380319 [Multi-domain]  Cd Length: 187  Bit Score: 37.07  E-value: 3.56e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*.
gi 32263325  20 NKKIPKETFEEILEVGRLAPSSFGMEPTRLLVLRSDEAKKALRPLC 65
Cdd:cd02143  13 DKPVPRETLEKLLDIARYAPTGHNSQPVHWLVVDDPEKVRRLAELV 58
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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