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Conserved domains on  [gi|21626729|gb|AAM68307|]
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speck, isoform A [Drosophila melanogaster]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11418877)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 146-215 9.86e-06

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 43.11  E-value: 9.86e-06
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 21626729 146 LSVDTNYRGLGIAGRLTERAYEYMRENGIN--VYHVLCSSHYSARVMEKLGFHEVFRmqFADYKPQGEVVFK 215
Cdd:COG0456  19 LAVDPEYRGRGIGRALLEAALERARERGARrlRLEVREDNEAAIALYEKLGFEEVGE--RPNYYGDDALVME 88
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 146-215 9.86e-06

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 43.11  E-value: 9.86e-06
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 21626729 146 LSVDTNYRGLGIAGRLTERAYEYMRENGIN--VYHVLCSSHYSARVMEKLGFHEVFRmqFADYKPQGEVVFK 215
Cdd:COG0456  19 LAVDPEYRGRGIGRALLEAALERARERGARrlRLEVREDNEAAIALYEKLGFEEVGE--RPNYYGDDALVME 88
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 146-195 2.85e-05

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 42.12  E-value: 2.85e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 21626729   146 LSVDTNYRGLGIAGRLTERAYEYMRENGINVY--HVLCSSHYSARVMEKLGF 195
Cdd:pfam00583  65 LAVAPEYRGKGIGTALLQALLEWARERGCERIflEVAADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 146-179 4.85e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 34.56  E-value: 4.85e-03
                        10        20        30
                ....*....|....*....|....*....|....
gi 21626729 146 LSVDTNYRGLGIAGRLTERAYEYMRENGINVYHV 179
Cdd:cd04301  31 LAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 146-215 9.86e-06

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 43.11  E-value: 9.86e-06
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 21626729 146 LSVDTNYRGLGIAGRLTERAYEYMRENGIN--VYHVLCSSHYSARVMEKLGFHEVFRmqFADYKPQGEVVFK 215
Cdd:COG0456  19 LAVDPEYRGRGIGRALLEAALERARERGARrlRLEVREDNEAAIALYEKLGFEEVGE--RPNYYGDDALVME 88
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 93-214 1.56e-05

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 43.44  E-value: 1.56e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21626729  93 MRRPSPDDVPEkaadscehpkfkkILSLMDHVEEQFNIFDVY---PDEELI-------LDGKI-----LSVDTNYRGLGI 157
Cdd:COG1246   3 IRPATPDDVPA-------------ILELIRPYALEEEIGEFWvaeEDGEIVgcaalhpLDEDLaelrsLAVHPDYRGRGI 69

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 21626729 158 AGRLTERAYEYMRENGIN-VYhvLCSSHYSARVMEKLGFHEVFRMQFADYKPQ--GEVVF 214
Cdd:COG1246  70 GRRLLEALLAEARELGLKrLF--LLTTSAAIHFYEKLGFEEIDKEDLPYAKVWqrDSVVM 127
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
148-192 2.66e-05

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 41.68  E-value: 2.66e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*
gi 21626729 148 VDTNYRGLGIAGRLTERAYEYMRENGINVYhVLCSshYSARVMEK 192
Cdd:COG2388  40 VPPALRGQGIASALVEAALDDARERGLKVV-PLCP--FVAAYFER 81
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 146-195 2.85e-05

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 42.12  E-value: 2.85e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 21626729   146 LSVDTNYRGLGIAGRLTERAYEYMRENGINVY--HVLCSSHYSARVMEKLGF 195
Cdd:pfam00583  65 LAVAPEYRGKGIGTALLQALLEWARERGCERIflEVAADNLAAIALYEKLGF 116
Acetyltransf_CG pfam14542
GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both ...
 148-192 3.73e-05

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.


Pssm-ID: 434030 [Multi-domain]  Cd Length: 79  Bit Score: 40.97  E-value: 3.73e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 21626729   148 VDTNYRGLGIAGRLTERAYEYMRENGINVyHVLCSshYSARVMEK 192
Cdd:pfam14542  31 VPPALRGQGIASKLVKAALDDAREEGLKI-VPLCS--YVAAYLEK 72
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 146-205 7.99e-05

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 41.10  E-value: 7.99e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 21626729   146 LSVDTNYRGLGIAGRLTERAYEYMRENGINVYHVLCSSH-YSARVMEKLGFHEVFRMQFAD 205
Cdd:pfam13673  57 LFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSELTVNASpYAVPFYEKLGFRATGPEQEFN 117
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 146-197 1.18e-04

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 39.75  E-value: 1.18e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 21626729   146 LSVDTNYRGLGIAGRLTERAYEYMRENGINVYHVlcSSHYSARVM-EKLGFHE 197
Cdd:pfam13508  34 LAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLEL--ETTNRAAAFyEKLGFEE 84
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
146-206 2.39e-04

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 40.07  E-value: 2.39e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 21626729 146 LSVDTNYRGLGIAGRLTERAYEYMRENGINVYHVLCSSH----YsarvmEKLGFHEVFRMQFADY 206
Cdd:COG3153  73 LAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSllpfY-----ERFGFRPAGELGLTLG 132
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 134-201 2.77e-04

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 39.65  E-value: 2.77e-04
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21626729 134 YPDEELILDGkiLSVDTNYRGLGIAGRLTERAYEYMRENGINVYHVLCSSHY--SARVMEKLGFHEVFRM 201
Cdd:COG0454  54 LDDKVLELKR--LYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNpaAIRFYERLGFKEIERY 121
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
148-208 4.60e-04

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 39.59  E-value: 4.60e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 21626729 148 VDTNYRGLGIAGRLTERAYEYMRENGIN--VYHVLCSSHYSARVMEKLGFHEVFRMQFADYKP 208
Cdd:COG1247  88 VDPDARGRGIGRALLEALIERARARGYRrlVAVVLADNEASIALYEKLGFEEVGTLPEVGFKF 150
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
148-208 5.45e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 37.97  E-value: 5.45e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 21626729 148 VDTNYRGLGIAGRLTERAYEYMRENGIN--VYHVLCSSHYSARVMEKLGFHEVFRMQFADYKP 208
Cdd:COG3393  23 THPEYRGRGLASALVAALAREALARGARtpFLYVDADNPAARRLYERLGFRPVGEYATVLFRK 85
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 147-195 3.08e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 36.40  E-value: 3.08e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 21626729   147 SVDTNYRGLGIAGRLTERAYEYMRENGINVYhVLCSSHYsaRVMEKLGF 195
Cdd:pfam13527  77 ATYPEYRGRGVMSRLLRRSLEEMRERGVPLS-FLYPSSY--PIYRRFGY 122
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 146-179 4.85e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 34.56  E-value: 4.85e-03
                        10        20        30
                ....*....|....*....|....*....|....
gi 21626729 146 LSVDTNYRGLGIAGRLTERAYEYMRENGINVYHV 179
Cdd:cd04301  31 LAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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