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Conserved domains on  [gi|62204389|gb|AAH92871|]
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Si:ch211-234f20.3 protein, partial [Danio rerio]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Stealth_CR2 pfam11380
Stealth protein CR2, conserved region 2; Stealth_CR2 is the second of several highly conserved ...
 327-434 9.20e-69

Stealth protein CR2, conserved region 2; Stealth_CR2 is the second of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR2 carries a well-conserved NDD sequence-motif. The domain is found in tandem with CR1, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.


:

Pssm-ID: 463267  Cd Length: 108  Bit Score: 221.19  E-value: 9.20e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389   327 RFEDNEELRYSLRSIEKHAPWVRHIFIVTNGQIPSWLNLDNPRVSVVTHQDIFQNQTHLPTFSSPAIETHIHRIPGLSQK 406
Cdd:pfam11380   1 RFRDRDELRYSLRSVEKYAPWVRKIYIVTDGQIPSWLNTSHPKIRIVDHKDIFPNESHLPTFNSHAIESNLHRIPGLSEH 80

                          90       100
                  ....*....|....*....|....*...
gi 62204389   407 FIYLNDDVMFGKDVWPDDFYSHSKGQKV 434
Cdd:pfam11380  81 FLYFNDDMFLGRPVKPEDFFTPDGLPKD 108
RRM1_GNPTAB cd21599
RNA recognition motif 1 (RRM1) found in N-acetylglucosamine-1-phosphotransferase subunits ...
 142-230 1.28e-30

RNA recognition motif 1 (RRM1) found in N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTAB) and similar proteins; GNPTAB, also termed GlcNAc-1-phosphotransferase subunits alpha/beta, or stealth protein GNPTAB, or UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta, catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment. The model corresponds to the RNA recognition motif 1 (RRM1) of GNPTAB. Its functional significance remains to be investigated.


:

Pssm-ID: 410178 [Multi-domain]  Cd Length: 90  Bit Score: 115.47  E-value: 1.28e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389 142 PVLVLDPALPANITVKELPTLSAAFSSAKELLQVAKPLHPSSSVTALLFHSHTEAEKAHADALK-DLLTHSISRGYLTTD 220
Cdd:cd21599   1 PMLVLDPALPENITLEDLREIYPAFKSATKIFNVTKPTNPSTNVTVVVFSSQTEVDNALKEKLViNGKNHSVSRGYLTSD 80

                        90
                ....*....|
gi 62204389 221 KEAPGLVRMH 230
Cdd:cd21599  81 WTVPHSVLLK 90
GlcNAc-1_reg super family cl39776
Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal ...
 224-310 8.54e-29

Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase), an alpha2beta2gamma2 hexamer, mediates the initial step in the addition of the mannose 6-phosphate targeting signal on newly synthesized lysosomal enzymes. GNPTAB encodes the alpha and beta subunits of GlcNAc-1-phosphotransferase, and mutations in this gene cause the lysosomal storage disorders mucolipidosis II and III alpha-beta The alpha-beta subunits contain three identifiable domains separated by so-called spacer regions. This domain is part of the first spacer region, Spacer-1. Studies indicate that GlcNAc-1 lacking spacer-1 exhibits enhanced phosphorylation of several non-lysosomal glycoproteins, while the phosphorylation of lysosomal acid hydrolases is not altered. In view of these effects on the maturation and function of GlcNAc-1, it is suggested to rename 'spacer-1' the 'regulatory-1' domain.


The actual alignment was detected with superfamily member pfam18440:

Pssm-ID: 408237  Cd Length: 88  Bit Score: 110.06  E-value: 8.54e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389   224 PGLVRMHTLAYLSGFPASLKETEQLRVKLPAVVTSKTKQLQLYSEASIALLHLNTAQDFTDLTQQAKKNLTLDGKELTVS 303
Cdd:pfam18440   2 PGLVLMQDLAFLSGFPRTFKETNQLKTKLPEKLRSKVKLLQLYSEASVALLDLNNPKDFDELLKQGKKNMTIDGKELTLT 81


                  ....*..
gi 62204389   304 PAFLFWD 310
Cdd:pfam18440  82 PAYLLWD 88
Notch pfam00066
LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch ...
 441-474 8.80e-13

LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch related proteins. The structure of the domain has been determined by NMR and was shown to contain three disulphide bonds and coordinate a calcium ion. Three repeats are also found in the PAPP-A peptidase.


:

Pssm-ID: 459658  Cd Length: 35  Bit Score: 62.93  E-value: 8.80e-13
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 62204389   441 PNC-AEGCPgSWIKDGYCDKACNNSACDWDGGDCQ 474
Cdd:pfam00066   2 PNCpYPYCW-DKFGNGVCDEECNNAECLWDGGDCS 35
Stealth_CR1 pfam17101
Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved ...
 78-99 5.93e-07

Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR1 carries a well-conserved IDVVYT sequence-motif. The domain is found in tandem with CR2, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.


:

Pssm-ID: 465346  Cd Length: 29  Bit Score: 46.31  E-value: 5.93e-07
                          10        20
                  ....*....|....*....|..
gi 62204389    78 MPIDVVYTWVNGTDTNLLKDLR 99
Cdd:pfam17101   1 FPIDVVYTWVNGSDPEWQAKKA 22
Notch pfam00066
LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch ...
 511-541 1.12e-06

LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch related proteins. The structure of the domain has been determined by NMR and was shown to contain three disulphide bonds and coordinate a calcium ion. Three repeats are also found in the PAPP-A peptidase.


:

Pssm-ID: 459658  Cd Length: 35  Bit Score: 45.60  E-value: 1.12e-06
                          10        20        30
                  ....*....|....*....|....*....|..
gi 62204389   511 CN-QGCANSWlADKFCDQACNVLACGFDVGDC 541
Cdd:pfam00066   4 CPyPYCWDKF-GNGVCDEECNNAECLWDGGDC 34
DMAP_binding super family cl05791
DMAP1-binding Domain; This domain binds DMAP1, a transcriptional co-repressor.
 699-739 3.23e-06

DMAP1-binding Domain; This domain binds DMAP1, a transcriptional co-repressor.


The actual alignment was detected with superfamily member pfam06464:

Pssm-ID: 368923 [Multi-domain]  Cd Length: 104  Bit Score: 46.26  E-value: 3.23e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 62204389   699 NETLLPDEVKIELKKLDEKLMSGDITIKGFNLTKAMLLEPY 739
Cdd:pfam06464   1 NPPSLPDDVRERLSELDLDLSEGDITEKGYEKKKLKLLRKF 41
 
Name Accession Description Interval E-value
Stealth_CR2 pfam11380
Stealth protein CR2, conserved region 2; Stealth_CR2 is the second of several highly conserved ...
 327-434 9.20e-69

Stealth protein CR2, conserved region 2; Stealth_CR2 is the second of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR2 carries a well-conserved NDD sequence-motif. The domain is found in tandem with CR1, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.


Pssm-ID: 463267  Cd Length: 108  Bit Score: 221.19  E-value: 9.20e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389   327 RFEDNEELRYSLRSIEKHAPWVRHIFIVTNGQIPSWLNLDNPRVSVVTHQDIFQNQTHLPTFSSPAIETHIHRIPGLSQK 406
Cdd:pfam11380   1 RFRDRDELRYSLRSVEKYAPWVRKIYIVTDGQIPSWLNTSHPKIRIVDHKDIFPNESHLPTFNSHAIESNLHRIPGLSEH 80

                          90       100
                  ....*....|....*....|....*...
gi 62204389   407 FIYLNDDVMFGKDVWPDDFYSHSKGQKV 434
Cdd:pfam11380  81 FLYFNDDMFLGRPVKPEDFFTPDGLPKD 108
RRM1_GNPTAB cd21599
RNA recognition motif 1 (RRM1) found in N-acetylglucosamine-1-phosphotransferase subunits ...
 142-230 1.28e-30

RNA recognition motif 1 (RRM1) found in N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTAB) and similar proteins; GNPTAB, also termed GlcNAc-1-phosphotransferase subunits alpha/beta, or stealth protein GNPTAB, or UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta, catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment. The model corresponds to the RNA recognition motif 1 (RRM1) of GNPTAB. Its functional significance remains to be investigated.


Pssm-ID: 410178 [Multi-domain]  Cd Length: 90  Bit Score: 115.47  E-value: 1.28e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389 142 PVLVLDPALPANITVKELPTLSAAFSSAKELLQVAKPLHPSSSVTALLFHSHTEAEKAHADALK-DLLTHSISRGYLTTD 220
Cdd:cd21599   1 PMLVLDPALPENITLEDLREIYPAFKSATKIFNVTKPTNPSTNVTVVVFSSQTEVDNALKEKLViNGKNHSVSRGYLTSD 80

                        90
                ....*....|
gi 62204389 221 KEAPGLVRMH 230
Cdd:cd21599  81 WTVPHSVLLK 90
GlcNAc-1_reg pfam18440
Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal ...
 224-310 8.54e-29

Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase), an alpha2beta2gamma2 hexamer, mediates the initial step in the addition of the mannose 6-phosphate targeting signal on newly synthesized lysosomal enzymes. GNPTAB encodes the alpha and beta subunits of GlcNAc-1-phosphotransferase, and mutations in this gene cause the lysosomal storage disorders mucolipidosis II and III alpha-beta The alpha-beta subunits contain three identifiable domains separated by so-called spacer regions. This domain is part of the first spacer region, Spacer-1. Studies indicate that GlcNAc-1 lacking spacer-1 exhibits enhanced phosphorylation of several non-lysosomal glycoproteins, while the phosphorylation of lysosomal acid hydrolases is not altered. In view of these effects on the maturation and function of GlcNAc-1, it is suggested to rename 'spacer-1' the 'regulatory-1' domain.


Pssm-ID: 408237  Cd Length: 88  Bit Score: 110.06  E-value: 8.54e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389   224 PGLVRMHTLAYLSGFPASLKETEQLRVKLPAVVTSKTKQLQLYSEASIALLHLNTAQDFTDLTQQAKKNLTLDGKELTVS 303
Cdd:pfam18440   2 PGLVLMQDLAFLSGFPRTFKETNQLKTKLPEKLRSKVKLLQLYSEASVALLDLNNPKDFDELLKQGKKNMTIDGKELTLT 81


                  ....*..
gi 62204389   304 PAFLFWD 310
Cdd:pfam18440  82 PAYLLWD 88
RRM2_GNPTAB cd21600
RNA recognition motif 2 (RRM2) found in N-acetylglucosamine-1-phosphotransferase subunits ...
 231-310 7.58e-25

RNA recognition motif 2 (RRM2) found in N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTAB) and similar proteins; GNPTAB, also termed GlcNAc-1-phosphotransferase subunits alpha/beta, or stealth protein GNPTAB, or UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta, catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment. The model corresponds to the RNA recognition motif 2 (RRM2) of GNPTAB. Its functional significance remains to be investigated.


Pssm-ID: 410179  Cd Length: 77  Bit Score: 98.45  E-value: 7.58e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389 231 TLAYLSGFPASLkETEQLRVKLPAVVTSKTKQLQLYSEASIALLHLNTAQDFTDLTQQakKNLTLDGKELTVSPAFLFWD 310
Cdd:cd21600   1 DTIMMSGIPSTY-EEEQLLAKLPETIRSKISKVELYSEKGVAVLTLSDKKAFDELLKL--SNMTIDGKEITLSPAYLVWD 77
Notch pfam00066
LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch ...
 441-474 8.80e-13

LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch related proteins. The structure of the domain has been determined by NMR and was shown to contain three disulphide bonds and coordinate a calcium ion. Three repeats are also found in the PAPP-A peptidase.


Pssm-ID: 459658  Cd Length: 35  Bit Score: 62.93  E-value: 8.80e-13
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 62204389   441 PNC-AEGCPgSWIKDGYCDKACNNSACDWDGGDCQ 474
Cdd:pfam00066   2 PNCpYPYCW-DKFGNGVCDEECNNAECLWDGGDCS 35
NL smart00004
Domain found in Notch and Lin-12; The Notch protein is essential for the proper ...
 439-473 1.57e-09

Domain found in Notch and Lin-12; The Notch protein is essential for the proper differentiation of the Drosophila ectoderm. This protein contains 3 NL domains.


Pssm-ID: 197463  Cd Length: 38  Bit Score: 53.48  E-value: 1.57e-09
                           10        20        30
                   ....*....|....*....|....*....|....*...
gi 62204389    439 PVPNC-AEGCpgsW--IKDGYCDKACNNSACDWDGGDC 473
Cdd:smart00004   4 PWSRCeDAQC---WdkFGDGVCDEECNNAECLWDGGDC 38
Stealth_CR1 pfam17101
Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved ...
 78-99 5.93e-07

Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR1 carries a well-conserved IDVVYT sequence-motif. The domain is found in tandem with CR2, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.


Pssm-ID: 465346  Cd Length: 29  Bit Score: 46.31  E-value: 5.93e-07
                          10        20
                  ....*....|....*....|..
gi 62204389    78 MPIDVVYTWVNGTDTNLLKDLR 99
Cdd:pfam17101   1 FPIDVVYTWVNGSDPEWQAKKA 22
Notch pfam00066
LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch ...
 511-541 1.12e-06

LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch related proteins. The structure of the domain has been determined by NMR and was shown to contain three disulphide bonds and coordinate a calcium ion. Three repeats are also found in the PAPP-A peptidase.


Pssm-ID: 459658  Cd Length: 35  Bit Score: 45.60  E-value: 1.12e-06
                          10        20        30
                  ....*....|....*....|....*....|..
gi 62204389   511 CN-QGCANSWlADKFCDQACNVLACGFDVGDC 541
Cdd:pfam00066   4 CPyPYCWDKF-GNGVCDEECNNAECLWDGGDC 34
DMAP_binding pfam06464
DMAP1-binding Domain; This domain binds DMAP1, a transcriptional co-repressor.
 699-739 3.23e-06

DMAP1-binding Domain; This domain binds DMAP1, a transcriptional co-repressor.


Pssm-ID: 368923 [Multi-domain]  Cd Length: 104  Bit Score: 46.26  E-value: 3.23e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 62204389   699 NETLLPDEVKIELKKLDEKLMSGDITIKGFNLTKAMLLEPY 739
Cdd:pfam06464   1 NPPSLPDDVRERLSELDLDLSEGDITEKGYEKKKLKLLRKF 41
NL smart00004
Domain found in Notch and Lin-12; The Notch protein is essential for the proper ...
 521-541 9.36e-05

Domain found in Notch and Lin-12; The Notch protein is essential for the proper differentiation of the Drosophila ectoderm. This protein contains 3 NL domains.


Pssm-ID: 197463  Cd Length: 38  Bit Score: 40.00  E-value: 9.36e-05
                           10        20
                   ....*....|....*....|.
gi 62204389    521 ADKFCDQACNVLACGFDVGDC 541
Cdd:smart00004  18 GDGVCDEECNNAECLWDGGDC 38
 
Name Accession Description Interval E-value
Stealth_CR2 pfam11380
Stealth protein CR2, conserved region 2; Stealth_CR2 is the second of several highly conserved ...
 327-434 9.20e-69

Stealth protein CR2, conserved region 2; Stealth_CR2 is the second of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR2 carries a well-conserved NDD sequence-motif. The domain is found in tandem with CR1, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.


Pssm-ID: 463267  Cd Length: 108  Bit Score: 221.19  E-value: 9.20e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389   327 RFEDNEELRYSLRSIEKHAPWVRHIFIVTNGQIPSWLNLDNPRVSVVTHQDIFQNQTHLPTFSSPAIETHIHRIPGLSQK 406
Cdd:pfam11380   1 RFRDRDELRYSLRSVEKYAPWVRKIYIVTDGQIPSWLNTSHPKIRIVDHKDIFPNESHLPTFNSHAIESNLHRIPGLSEH 80

                          90       100
                  ....*....|....*....|....*...
gi 62204389   407 FIYLNDDVMFGKDVWPDDFYSHSKGQKV 434
Cdd:pfam11380  81 FLYFNDDMFLGRPVKPEDFFTPDGLPKD 108
RRM1_GNPTAB cd21599
RNA recognition motif 1 (RRM1) found in N-acetylglucosamine-1-phosphotransferase subunits ...
 142-230 1.28e-30

RNA recognition motif 1 (RRM1) found in N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTAB) and similar proteins; GNPTAB, also termed GlcNAc-1-phosphotransferase subunits alpha/beta, or stealth protein GNPTAB, or UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta, catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment. The model corresponds to the RNA recognition motif 1 (RRM1) of GNPTAB. Its functional significance remains to be investigated.


Pssm-ID: 410178 [Multi-domain]  Cd Length: 90  Bit Score: 115.47  E-value: 1.28e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389 142 PVLVLDPALPANITVKELPTLSAAFSSAKELLQVAKPLHPSSSVTALLFHSHTEAEKAHADALK-DLLTHSISRGYLTTD 220
Cdd:cd21599   1 PMLVLDPALPENITLEDLREIYPAFKSATKIFNVTKPTNPSTNVTVVVFSSQTEVDNALKEKLViNGKNHSVSRGYLTSD 80

                        90
                ....*....|
gi 62204389 221 KEAPGLVRMH 230
Cdd:cd21599  81 WTVPHSVLLK 90
GlcNAc-1_reg pfam18440
Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal ...
 224-310 8.54e-29

Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase), an alpha2beta2gamma2 hexamer, mediates the initial step in the addition of the mannose 6-phosphate targeting signal on newly synthesized lysosomal enzymes. GNPTAB encodes the alpha and beta subunits of GlcNAc-1-phosphotransferase, and mutations in this gene cause the lysosomal storage disorders mucolipidosis II and III alpha-beta The alpha-beta subunits contain three identifiable domains separated by so-called spacer regions. This domain is part of the first spacer region, Spacer-1. Studies indicate that GlcNAc-1 lacking spacer-1 exhibits enhanced phosphorylation of several non-lysosomal glycoproteins, while the phosphorylation of lysosomal acid hydrolases is not altered. In view of these effects on the maturation and function of GlcNAc-1, it is suggested to rename 'spacer-1' the 'regulatory-1' domain.


Pssm-ID: 408237  Cd Length: 88  Bit Score: 110.06  E-value: 8.54e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389   224 PGLVRMHTLAYLSGFPASLKETEQLRVKLPAVVTSKTKQLQLYSEASIALLHLNTAQDFTDLTQQAKKNLTLDGKELTVS 303
Cdd:pfam18440   2 PGLVLMQDLAFLSGFPRTFKETNQLKTKLPEKLRSKVKLLQLYSEASVALLDLNNPKDFDELLKQGKKNMTIDGKELTLT 81


                  ....*..
gi 62204389   304 PAFLFWD 310
Cdd:pfam18440  82 PAYLLWD 88
RRM2_GNPTAB cd21600
RNA recognition motif 2 (RRM2) found in N-acetylglucosamine-1-phosphotransferase subunits ...
 231-310 7.58e-25

RNA recognition motif 2 (RRM2) found in N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTAB) and similar proteins; GNPTAB, also termed GlcNAc-1-phosphotransferase subunits alpha/beta, or stealth protein GNPTAB, or UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta, catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment. The model corresponds to the RNA recognition motif 2 (RRM2) of GNPTAB. Its functional significance remains to be investigated.


Pssm-ID: 410179  Cd Length: 77  Bit Score: 98.45  E-value: 7.58e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62204389 231 TLAYLSGFPASLkETEQLRVKLPAVVTSKTKQLQLYSEASIALLHLNTAQDFTDLTQQakKNLTLDGKELTVSPAFLFWD 310
Cdd:cd21600   1 DTIMMSGIPSTY-EEEQLLAKLPETIRSKISKVELYSEKGVAVLTLSDKKAFDELLKL--SNMTIDGKEITLSPAYLVWD 77
Notch pfam00066
LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch ...
 441-474 8.80e-13

LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch related proteins. The structure of the domain has been determined by NMR and was shown to contain three disulphide bonds and coordinate a calcium ion. Three repeats are also found in the PAPP-A peptidase.


Pssm-ID: 459658  Cd Length: 35  Bit Score: 62.93  E-value: 8.80e-13
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 62204389   441 PNC-AEGCPgSWIKDGYCDKACNNSACDWDGGDCQ 474
Cdd:pfam00066   2 PNCpYPYCW-DKFGNGVCDEECNNAECLWDGGDCS 35
NL smart00004
Domain found in Notch and Lin-12; The Notch protein is essential for the proper ...
 439-473 1.57e-09

Domain found in Notch and Lin-12; The Notch protein is essential for the proper differentiation of the Drosophila ectoderm. This protein contains 3 NL domains.


Pssm-ID: 197463  Cd Length: 38  Bit Score: 53.48  E-value: 1.57e-09
                           10        20        30
                   ....*....|....*....|....*....|....*...
gi 62204389    439 PVPNC-AEGCpgsW--IKDGYCDKACNNSACDWDGGDC 473
Cdd:smart00004   4 PWSRCeDAQC---WdkFGDGVCDEECNNAECLWDGGDC 38
Stealth_CR1 pfam17101
Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved ...
 78-99 5.93e-07

Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR1 carries a well-conserved IDVVYT sequence-motif. The domain is found in tandem with CR2, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.


Pssm-ID: 465346  Cd Length: 29  Bit Score: 46.31  E-value: 5.93e-07
                          10        20
                  ....*....|....*....|..
gi 62204389    78 MPIDVVYTWVNGTDTNLLKDLR 99
Cdd:pfam17101   1 FPIDVVYTWVNGSDPEWQAKKA 22
Notch pfam00066
LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch ...
 511-541 1.12e-06

LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch related proteins. The structure of the domain has been determined by NMR and was shown to contain three disulphide bonds and coordinate a calcium ion. Three repeats are also found in the PAPP-A peptidase.


Pssm-ID: 459658  Cd Length: 35  Bit Score: 45.60  E-value: 1.12e-06
                          10        20        30
                  ....*....|....*....|....*....|..
gi 62204389   511 CN-QGCANSWlADKFCDQACNVLACGFDVGDC 541
Cdd:pfam00066   4 CPyPYCWDKF-GNGVCDEECNNAECLWDGGDC 34
DMAP_binding pfam06464
DMAP1-binding Domain; This domain binds DMAP1, a transcriptional co-repressor.
 699-739 3.23e-06

DMAP1-binding Domain; This domain binds DMAP1, a transcriptional co-repressor.


Pssm-ID: 368923 [Multi-domain]  Cd Length: 104  Bit Score: 46.26  E-value: 3.23e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 62204389   699 NETLLPDEVKIELKKLDEKLMSGDITIKGFNLTKAMLLEPY 739
Cdd:pfam06464   1 NPPSLPDDVRERLSELDLDLSEGDITEKGYEKKKLKLLRKF 41
NL smart00004
Domain found in Notch and Lin-12; The Notch protein is essential for the proper ...
 521-541 9.36e-05

Domain found in Notch and Lin-12; The Notch protein is essential for the proper differentiation of the Drosophila ectoderm. This protein contains 3 NL domains.


Pssm-ID: 197463  Cd Length: 38  Bit Score: 40.00  E-value: 9.36e-05
                           10        20
                   ....*....|....*....|.
gi 62204389    521 ADKFCDQACNVLACGFDVGDC 541
Cdd:smart00004  18 GDGVCDEECNNAECLWDGGDC 38
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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