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Conserved domains on  [gi|254574906|pdb|3H0L|X]
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Chain X, Glutamyl-tRNA(Gln) amidotransferase subunit C

Protein Classification

Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC( domain architecture ID 10002289)

Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC is part of a heterotrimeric complex that forms correctly charged Gln-tRNA(Gln) or Asn-tRNA(Asn) through the transamidation of misacylated Glu-tRNA(Gln) or Asp-tRNA(Asn)

CATH:  1.10.20.60
EC:  6.3.5.-
Gene Ontology:  GO:0006450|GO:0050567|GO:0005524
SCOP:  3001870

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GatC COG0721
Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit [Translation, ribosomal structure and ...
 1-93 1.71e-39

Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit [Translation, ribosomal structure and biogenesis]; Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


:

Pssm-ID: 440485 [Multi-domain]  Cd Length: 95  Bit Score: 125.98  E-value: 1.71e-39
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X       1 MVDREWVLKIAKLARLELKEEEIEVFQKQLSDILDFIDQLKELDTENVEPYIQEFEET-PMREDEPHPSLDREKALMNAP 79
Cdd:COG0721  2 SITKEEVEHIAKLARLELSEEELERLAGQLNDILDYVEQLNEVDTEGVEPTAHPLDLTnVLREDEVTESLDREEALANAP 81

                       90
               ....*....|....
3H0L_X      80 ERKDGFFVVPRVVE 93
Cdd:COG0721 82 ETEDGYFKVPKVIE 95
 
Name Accession Description Interval E-value
GatC COG0721
Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit [Translation, ribosomal structure and ...
 1-93 1.71e-39

Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit [Translation, ribosomal structure and biogenesis]; Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 440485 [Multi-domain]  Cd Length: 95  Bit Score: 125.98  E-value: 1.71e-39
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X       1 MVDREWVLKIAKLARLELKEEEIEVFQKQLSDILDFIDQLKELDTENVEPYIQEFEET-PMREDEPHPSLDREKALMNAP 79
Cdd:COG0721  2 SITKEEVEHIAKLARLELSEEELERLAGQLNDILDYVEQLNEVDTEGVEPTAHPLDLTnVLREDEVTESLDREEALANAP 81

                       90
               ....*....|....
3H0L_X      80 ERKDGFFVVPRVVE 93
Cdd:COG0721 82 ETEDGYFKVPKVIE 95
gatC PRK00034
Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC;
1-93 3.19e-32

Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC;


Pssm-ID: 178810 [Multi-domain]  Cd Length: 95  Bit Score: 107.59  E-value: 3.19e-32
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X        1 MVDREWVLKIAKLARLELKEEEIEVFQKQLSDILDFIDQLKELDTENVEP--YIQEFeETPMREDEPHPSLDREKALMNA 78
Cdd:PRK00034  2 AITREEVKHLAKLARLELSEEELEKFAGQLNKILDFVEQLNEVDTEGVEPttHPLDM-KNVLREDVVTESLPREEALKNA 80

                        90
                ....*....|....*
3H0L_X       79 PERKDGFFVVPRVVE 93
Cdd:PRK00034 81 PESEDGYFKVPKVIE 95
gatC TIGR00135
aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, C subunit; Archaea, organelles, and many ...
 2-93 2.63e-25

aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, C subunit; Archaea, organelles, and many bacteria charge Gln-tRNA by first misacylating it with Glu and then amidating Glu to Gln. This small protein is part of the amidotransferase heterotrimer and appears to be important to the stability of the amidase subunit encode by gatA, but its function may not be required in every organism that expresses gatA and gatB. The seed alignment for this model does not include any eukaryotic sequence and is not guaranteed to find eukaryotic examples, although it does find some. Saccharomyces cerevisiae, which expresses the amidotransferase for mitochondrial protein translation, seems to lack a gatC ortholog. This model has been revised to remove the candidate sequence from Methanococcus jannaschii, now part of a related model. [Protein synthesis, tRNA aminoacylation]


Pssm-ID: 129241 [Multi-domain]  Cd Length: 93  Bit Score: 90.05  E-value: 2.63e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X         2 VDREWVLKIAKLARLELKEEEIEVFQKQLSDILDFIDQLKELDTENVEPYIQEFE-ETPMREDEPHPSLDREKALMNAPE 80
Cdd:TIGR00135  1 ISDEEVKHLAKLARLELSEEEAESFAGDLDKILGFVEQLNEVDTENVEPMTHPLEiSNVLREDEPEEPLSRDDILKNAPE 80

                         90
                 ....*....|...
3H0L_X        81 RKDGFFVVPRVVE 93
Cdd:TIGR00135 81 KEDGFIKVPKIIE 93
Glu-tRNAGln pfam02686
Glu-tRNAGln amidotransferase C subunit; This is a family of Glu-tRNAGln amidotransferase C ...
 20-88 2.99e-25

Glu-tRNAGln amidotransferase C subunit; This is a family of Glu-tRNAGln amidotransferase C subunits. The Glu-tRNA Gln amidotransferase enzyme itself is an important translational fidelity mechanism replacing incorrectly charged Glu-tRNAGln with the correct Gln-tRANGln via transmidation of the misacylated Glu-tRNAGln. This activity supplements the lack of glutaminyl-tRNA synthetase activity in gram-positive eubacterteria, cyanobacteria, Archaea, and organelles.


Pssm-ID: 460651 [Multi-domain]  Cd Length: 70  Bit Score: 89.11  E-value: 2.99e-25
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X        20 EEEIEVFQKQLSDILDFIDQLKELDTENVEPYIQEFEET-PMREDEPHPSLDREKALMNAPERKDGFFVV 88
Cdd:pfam02686  1 EEELEEFAKQLNDILDYVEQLNEVDTEGVEPTSHPLDLTnVLREDEVTESLDREEALANAPETEDGFFKV 70
 
Name Accession Description Interval E-value
GatC COG0721
Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit [Translation, ribosomal structure and ...
 1-93 1.71e-39

Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit [Translation, ribosomal structure and biogenesis]; Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 440485 [Multi-domain]  Cd Length: 95  Bit Score: 125.98  E-value: 1.71e-39
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X       1 MVDREWVLKIAKLARLELKEEEIEVFQKQLSDILDFIDQLKELDTENVEPYIQEFEET-PMREDEPHPSLDREKALMNAP 79
Cdd:COG0721  2 SITKEEVEHIAKLARLELSEEELERLAGQLNDILDYVEQLNEVDTEGVEPTAHPLDLTnVLREDEVTESLDREEALANAP 81

                       90
               ....*....|....
3H0L_X      80 ERKDGFFVVPRVVE 93
Cdd:COG0721 82 ETEDGYFKVPKVIE 95
gatC PRK00034
Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC;
1-93 3.19e-32

Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC;


Pssm-ID: 178810 [Multi-domain]  Cd Length: 95  Bit Score: 107.59  E-value: 3.19e-32
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X        1 MVDREWVLKIAKLARLELKEEEIEVFQKQLSDILDFIDQLKELDTENVEP--YIQEFeETPMREDEPHPSLDREKALMNA 78
Cdd:PRK00034  2 AITREEVKHLAKLARLELSEEELEKFAGQLNKILDFVEQLNEVDTEGVEPttHPLDM-KNVLREDVVTESLPREEALKNA 80

                        90
                ....*....|....*
3H0L_X       79 PERKDGFFVVPRVVE 93
Cdd:PRK00034 81 PESEDGYFKVPKVIE 95
gatC TIGR00135
aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, C subunit; Archaea, organelles, and many ...
 2-93 2.63e-25

aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, C subunit; Archaea, organelles, and many bacteria charge Gln-tRNA by first misacylating it with Glu and then amidating Glu to Gln. This small protein is part of the amidotransferase heterotrimer and appears to be important to the stability of the amidase subunit encode by gatA, but its function may not be required in every organism that expresses gatA and gatB. The seed alignment for this model does not include any eukaryotic sequence and is not guaranteed to find eukaryotic examples, although it does find some. Saccharomyces cerevisiae, which expresses the amidotransferase for mitochondrial protein translation, seems to lack a gatC ortholog. This model has been revised to remove the candidate sequence from Methanococcus jannaschii, now part of a related model. [Protein synthesis, tRNA aminoacylation]


Pssm-ID: 129241 [Multi-domain]  Cd Length: 93  Bit Score: 90.05  E-value: 2.63e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X         2 VDREWVLKIAKLARLELKEEEIEVFQKQLSDILDFIDQLKELDTENVEPYIQEFE-ETPMREDEPHPSLDREKALMNAPE 80
Cdd:TIGR00135  1 ISDEEVKHLAKLARLELSEEEAESFAGDLDKILGFVEQLNEVDTENVEPMTHPLEiSNVLREDEPEEPLSRDDILKNAPE 80

                         90
                 ....*....|...
3H0L_X        81 RKDGFFVVPRVVE 93
Cdd:TIGR00135 81 KEDGFIKVPKIIE 93
Glu-tRNAGln pfam02686
Glu-tRNAGln amidotransferase C subunit; This is a family of Glu-tRNAGln amidotransferase C ...
 20-88 2.99e-25

Glu-tRNAGln amidotransferase C subunit; This is a family of Glu-tRNAGln amidotransferase C subunits. The Glu-tRNA Gln amidotransferase enzyme itself is an important translational fidelity mechanism replacing incorrectly charged Glu-tRNAGln with the correct Gln-tRANGln via transmidation of the misacylated Glu-tRNAGln. This activity supplements the lack of glutaminyl-tRNA synthetase activity in gram-positive eubacterteria, cyanobacteria, Archaea, and organelles.


Pssm-ID: 460651 [Multi-domain]  Cd Length: 70  Bit Score: 89.11  E-value: 2.99e-25
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X        20 EEEIEVFQKQLSDILDFIDQLKELDTENVEPYIQEFEET-PMREDEPHPSLDREKALMNAPERKDGFFVV 88
Cdd:pfam02686  1 EEELEEFAKQLNDILDYVEQLNEVDTEGVEPTSHPLDLTnVLREDEVTESLDREEALANAPETEDGFFKV 70
PRK12821 PRK12821
aspartyl/glutamyl-tRNA amidotransferase subunit C-like protein; Provisional
 2-88 1.49e-11

aspartyl/glutamyl-tRNA amidotransferase subunit C-like protein; Provisional


Pssm-ID: 237216 [Multi-domain]  Cd Length: 477  Bit Score: 58.75  E-value: 1.49e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3H0L_X         2 VDREWVLKIAKLARLELKEEEIEVFQKQLSDILDFIDQLKELDTENVEP--YIQEFEETPMREDEPHPSLDREKALMNAP 79
Cdd:PRK12821 389 LNKDELKKLARLVMFDLDDAELEKLQVEFKDITSSFKQVEKIDTTNVKPmyAPFSNSPTPLRKDKDVVQKHQKILLKNCK 468


                 ....*....
3H0L_X        80 ERKDGFFVV 88
Cdd:PRK12821 469 ETLGGFVKV 477
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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