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Conserved domains on  [gi|197725201|pdb|3D87|B]
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Chain B, Interleukin-12 subunit p40

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IL12p40_C pfam10420
Cytokine interleukin-12p40 C-terminus; IL12p40_C is the largely beta stranded C-terminal, D3, ...
 104-194 1.70e-24

Cytokine interleukin-12p40 C-terminus; IL12p40_C is the largely beta stranded C-terminal, D3, domain of interleukin-12p40 or interleukin-12B. This interleukin is produced on stimulation by macrophage-engulfed micro-organizms and other stimuli, when it dimerizes with interleukin-12p35 to form a heterodimer which then binds to receptors on natural killer cells to activate them to destroy the micro-organizms. This domain contains two disulfide bridges, one of which serves to bind p40 to p35 and the other to hold the beta strands within the domain together. The cupped shape of the p35 binding interface matches the elbow-like bend between D2 and D3 in p40. The domain is often associated with family fn3, pfam00041.


:

Pssm-ID: 463086  Cd Length: 80  Bit Score: 94.30  E-value: 1.70e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3D87_B        104 KTFLRCEAKNYSGRFTCWWLTTIS---TDLTFsVKSSRGsSDPQGVTCGAATLSaervrgdnkeyeYSVECQEDSACPAA 180
Cdd:pfam10420   1 KTFLKCEAKNYSGNFTCSWRWDSRrngADVKF-IKASRG-SGQQGVTCSLDGGG------------YGLTCQDHSFCPYA 66

                          90
                  ....*....|....
3D87_B        181 EESLPIEVMVDAVH 194
Cdd:pfam10420  67 EESHPIEVTLEAIS 80
FN3 cd00063
Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein ...
 213-303 7.20e-04

Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.


:

Pssm-ID: 238020 [Multi-domain]  Cd Length: 93  Bit Score: 38.25  E-value: 7.20e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3D87_B      213 PDPPKNLQLKPLkNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVftDKTSATVicrKNA------SISV 286
Cdd:cd00063   1 PSPPTNLRVTDV-TSTSVTLSWTPPEDDGGPITGYVVEYREKGSGDWKEVEVTPG--SETSYTL---TGLkpgteyEFRV 74

                        90
                ....*....|....*..
3D87_B      287 RAQDRYYSSSWSEWASV 303
Cdd:cd00063  75 RAVNGGGESPPSESVTV 91
Ig_2 pfam13895
Immunoglobulin domain; This domain contains immunoglobulin-like domains.
 21-74 1.68e-03

Immunoglobulin domain; This domain contains immunoglobulin-like domains.


:

Pssm-ID: 464026 [Multi-domain]  Cd Length: 79  Bit Score: 36.60  E-value: 1.68e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
3D87_B         21 GEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTC----HKGGEV 74
Cdd:pfam13895  14 GEPVTLTCSAPGNPPPSYTWYKDGSAISSSPNFFTLSVSAEDSGTYTCvarnGRGGKV 71
 
Name Accession Description Interval E-value
IL12p40_C pfam10420
Cytokine interleukin-12p40 C-terminus; IL12p40_C is the largely beta stranded C-terminal, D3, ...
 104-194 1.70e-24

Cytokine interleukin-12p40 C-terminus; IL12p40_C is the largely beta stranded C-terminal, D3, domain of interleukin-12p40 or interleukin-12B. This interleukin is produced on stimulation by macrophage-engulfed micro-organizms and other stimuli, when it dimerizes with interleukin-12p35 to form a heterodimer which then binds to receptors on natural killer cells to activate them to destroy the micro-organizms. This domain contains two disulfide bridges, one of which serves to bind p40 to p35 and the other to hold the beta strands within the domain together. The cupped shape of the p35 binding interface matches the elbow-like bend between D2 and D3 in p40. The domain is often associated with family fn3, pfam00041.


Pssm-ID: 463086  Cd Length: 80  Bit Score: 94.30  E-value: 1.70e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3D87_B        104 KTFLRCEAKNYSGRFTCWWLTTIS---TDLTFsVKSSRGsSDPQGVTCGAATLSaervrgdnkeyeYSVECQEDSACPAA 180
Cdd:pfam10420   1 KTFLKCEAKNYSGNFTCSWRWDSRrngADVKF-IKASRG-SGQQGVTCSLDGGG------------YGLTCQDHSFCPYA 66

                          90
                  ....*....|....
3D87_B        181 EESLPIEVMVDAVH 194
Cdd:pfam10420  67 EESHPIEVTLEAIS 80
FN3 cd00063
Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein ...
 213-303 7.20e-04

Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.


Pssm-ID: 238020 [Multi-domain]  Cd Length: 93  Bit Score: 38.25  E-value: 7.20e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3D87_B      213 PDPPKNLQLKPLkNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVftDKTSATVicrKNA------SISV 286
Cdd:cd00063   1 PSPPTNLRVTDV-TSTSVTLSWTPPEDDGGPITGYVVEYREKGSGDWKEVEVTPG--SETSYTL---TGLkpgteyEFRV 74

                        90
                ....*....|....*..
3D87_B      287 RAQDRYYSSSWSEWASV 303
Cdd:cd00063  75 RAVNGGGESPPSESVTV 91
Ig_2 pfam13895
Immunoglobulin domain; This domain contains immunoglobulin-like domains.
 21-74 1.68e-03

Immunoglobulin domain; This domain contains immunoglobulin-like domains.


Pssm-ID: 464026 [Multi-domain]  Cd Length: 79  Bit Score: 36.60  E-value: 1.68e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
3D87_B         21 GEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTC----HKGGEV 74
Cdd:pfam13895  14 GEPVTLTCSAPGNPPPSYTWYKDGSAISSSPNFFTLSVSAEDSGTYTCvarnGRGGKV 71
IgI_C2_MyBP-C-like cd20967
Domain C2 of human cardiac Myosin Binding Protein C and similar domains; a member of the I-set ...
 16-73 6.90e-03

Domain C2 of human cardiac Myosin Binding Protein C and similar domains; a member of the I-set of IgSF domains; The members here are composed of the immunoglobulin (Ig) Domain C2 of human cardiac Myosin Binding Protein C (MyBP-C) and similar domains. MyBP-C is a thick filament protein involved in the regulation of muscle contraction. Mutations in cardiac MyBP-C gene are the second most frequent cause of hypertrophic cardiomyopathy. MyBP-C binds to myosin with two binding sites, one at its C-terminus and another at its N-terminus. The N-terminal binding site, consisting of immunoglobulin (lg) domains C1 and C2 connected by a flexible linker, interacts with the S2 segment of myosin in a phosphorylation-regulated manner. The C1 and C2 Ig domains can bind to and activate or inhibit the thin filament. IgSF domains can be divided into 4 main classes based on their structures and sequences: the Variable (V), Constant 1 (C1), Constant 2 (C2), and Intermediate (I) sets. Unlike the V-set, one of the distinctive features of I-set domains is the lack of a C" strand. The structures of the Ig domains of MyBP-C lack this strand and thus belong to the I-set of Ig superfamily domains. I-set domains are found in several cell adhesion molecules (such as VCAM, ICAM, and MADCAM), and are also present in numerous other diverse protein families, including several tyrosine-protein kinase receptors.


Pssm-ID: 409559  Cd Length: 82  Bit Score: 34.91  E-value: 6.90e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
3D87_B       16 YPDAPGEMVVLTCDTPEEDG-ITWTLD----QSS-----EVLGSGKTLTIQVKEFGDAGQYTCHKGGE 73
Cdd:cd20967   7 VQVSKGHKIRLTVELADPDAeVKWYKDgqelQSSskvifESIGAKRTLTVQQASLADAGEYQCVAGGE 74
 
Name Accession Description Interval E-value
IL12p40_C pfam10420
Cytokine interleukin-12p40 C-terminus; IL12p40_C is the largely beta stranded C-terminal, D3, ...
 104-194 1.70e-24

Cytokine interleukin-12p40 C-terminus; IL12p40_C is the largely beta stranded C-terminal, D3, domain of interleukin-12p40 or interleukin-12B. This interleukin is produced on stimulation by macrophage-engulfed micro-organizms and other stimuli, when it dimerizes with interleukin-12p35 to form a heterodimer which then binds to receptors on natural killer cells to activate them to destroy the micro-organizms. This domain contains two disulfide bridges, one of which serves to bind p40 to p35 and the other to hold the beta strands within the domain together. The cupped shape of the p35 binding interface matches the elbow-like bend between D2 and D3 in p40. The domain is often associated with family fn3, pfam00041.


Pssm-ID: 463086  Cd Length: 80  Bit Score: 94.30  E-value: 1.70e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3D87_B        104 KTFLRCEAKNYSGRFTCWWLTTIS---TDLTFsVKSSRGsSDPQGVTCGAATLSaervrgdnkeyeYSVECQEDSACPAA 180
Cdd:pfam10420   1 KTFLKCEAKNYSGNFTCSWRWDSRrngADVKF-IKASRG-SGQQGVTCSLDGGG------------YGLTCQDHSFCPYA 66

                          90
                  ....*....|....
3D87_B        181 EESLPIEVMVDAVH 194
Cdd:pfam10420  67 EESHPIEVTLEAIS 80
FN3 cd00063
Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein ...
 213-303 7.20e-04

Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.


Pssm-ID: 238020 [Multi-domain]  Cd Length: 93  Bit Score: 38.25  E-value: 7.20e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3D87_B      213 PDPPKNLQLKPLkNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVftDKTSATVicrKNA------SISV 286
Cdd:cd00063   1 PSPPTNLRVTDV-TSTSVTLSWTPPEDDGGPITGYVVEYREKGSGDWKEVEVTPG--SETSYTL---TGLkpgteyEFRV 74

                        90
                ....*....|....*..
3D87_B      287 RAQDRYYSSSWSEWASV 303
Cdd:cd00063  75 RAVNGGGESPPSESVTV 91
Ig_2 pfam13895
Immunoglobulin domain; This domain contains immunoglobulin-like domains.
 21-74 1.68e-03

Immunoglobulin domain; This domain contains immunoglobulin-like domains.


Pssm-ID: 464026 [Multi-domain]  Cd Length: 79  Bit Score: 36.60  E-value: 1.68e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
3D87_B         21 GEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTC----HKGGEV 74
Cdd:pfam13895  14 GEPVTLTCSAPGNPPPSYTWYKDGSAISSSPNFFTLSVSAEDSGTYTCvarnGRGGKV 71
Ig_3 pfam13927
Immunoglobulin domain; This family contains immunoglobulin-like domains.
 20-68 4.97e-03

Immunoglobulin domain; This family contains immunoglobulin-like domains.


Pssm-ID: 464046 [Multi-domain]  Cd Length: 78  Bit Score: 35.23  E-value: 4.97e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
3D87_B         20 PGEMVVLTC-----DTPEedgITWTLD---------QSSEVLGSGKTLTIQVKEFGDAGQYTC 68
Cdd:pfam13927  15 EGETVTLTCeatgsPPPT---ITWYKNgepissgstRSRSLSGSNSTLTISNVTRSDAGTYTC 74
IgI_C2_MyBP-C-like cd20967
Domain C2 of human cardiac Myosin Binding Protein C and similar domains; a member of the I-set ...
 16-73 6.90e-03

Domain C2 of human cardiac Myosin Binding Protein C and similar domains; a member of the I-set of IgSF domains; The members here are composed of the immunoglobulin (Ig) Domain C2 of human cardiac Myosin Binding Protein C (MyBP-C) and similar domains. MyBP-C is a thick filament protein involved in the regulation of muscle contraction. Mutations in cardiac MyBP-C gene are the second most frequent cause of hypertrophic cardiomyopathy. MyBP-C binds to myosin with two binding sites, one at its C-terminus and another at its N-terminus. The N-terminal binding site, consisting of immunoglobulin (lg) domains C1 and C2 connected by a flexible linker, interacts with the S2 segment of myosin in a phosphorylation-regulated manner. The C1 and C2 Ig domains can bind to and activate or inhibit the thin filament. IgSF domains can be divided into 4 main classes based on their structures and sequences: the Variable (V), Constant 1 (C1), Constant 2 (C2), and Intermediate (I) sets. Unlike the V-set, one of the distinctive features of I-set domains is the lack of a C" strand. The structures of the Ig domains of MyBP-C lack this strand and thus belong to the I-set of Ig superfamily domains. I-set domains are found in several cell adhesion molecules (such as VCAM, ICAM, and MADCAM), and are also present in numerous other diverse protein families, including several tyrosine-protein kinase receptors.


Pssm-ID: 409559  Cd Length: 82  Bit Score: 34.91  E-value: 6.90e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
3D87_B       16 YPDAPGEMVVLTCDTPEEDG-ITWTLD----QSS-----EVLGSGKTLTIQVKEFGDAGQYTCHKGGE 73
Cdd:cd20967   7 VQVSKGHKIRLTVELADPDAeVKWYKDgqelQSSskvifESIGAKRTLTVQQASLADAGEYQCVAGGE 74
Ig_Semaphorin_C cd04979
Immunoglobulin (Ig)-like domain at the C-terminus of semaphorins; The members here are ...
 19-74 8.54e-03

Immunoglobulin (Ig)-like domain at the C-terminus of semaphorins; The members here are composed of the immunoglobulin (Ig)-like domain in semaphorins. Semaphorins are transmembrane protein that have important roles in a variety of tissues. Functionally, semaphorins were initially characterized for their importance in the development of the nervous system and in axonal guidance. Later they have been found to be important for the formation and functioning of the cardiovascular, endocrine, gastrointestinal, hepatic, immune, musculoskeletal, renal, reproductive, and respiratory systems. Semaphorins function through binding to their receptors and transmembrane semaphorins also serves as receptors themselves. Although molecular mechanism of semaphorins is poorly understood, the Ig-like domains may be involved in ligand binding or dimerization.


Pssm-ID: 409368  Cd Length: 88  Bit Score: 34.74  E-value: 8.54e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
3D87_B       19 APGEMVVLTCDTPEEDG-ITWTLDQSSE--------VLGSGKTLTIQVKEFGDAGQYTCHKGGEV 74
Cdd:cd04979   9 KEGDTVILSCSVKSNNApVTWIHNGKKVpryrsprlVLKTERGLLIRSAQEADAGVYECHSGERV 73
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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